Rroxscaffold_6G00394450

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005518
Physical Location & Seq
Reverse (-)
15071631 .. 15079614
7984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394450.1

Sequence Viewer

Length: 1956 bp
ATGATGAATAAGAAGATATTGACAACACTGTCAATAATTACGATCAGCCAGATATGGTGGTGGGCTTGTGTGAGTGCAGACCCGCAGATCAATCTTCTGAACGAGGGTTGCAGCCAGTACAACGCAACCAGCCTGTCCAACTTCTATACCAATCTCAACGCCACTTTCTTGGACCTCAAAGCGCAGCTACTCAACGGCAGCAAACACTTTGCAACGGCGCAACAGGCCAAGGGCTCCGACCCCGTCTACGCCATGACGCAGTGTAGGAACTACCTCTCCTCCGCCGACTGTCTATCTTGCTTTGCCGCCGCCGTCTCCCAAATCCGCAACTGCTCTGCCTCCAATGGCGCACGCGTCGTCTACGACGGCTGCTTCCTCCGGTATGAGAGCAGTGGTTTTTTTGACCAGACTGTAGCTACCCTTCCTGGTAATGTAGGACTCTGTGGGAATCAAACTGGATCGCCTGCTACAACCTTTACTGCAGCTGCTGAAGAACTTCTAATGGATCTTCAAGTTGCTACGCCCAAGATTAATGGCTTCTTTGCAGCATCTAAGGAGGTAGTTAGCGGTAGTGGAAACACAACAGTCTATGCTGTGGCACAATGTGCCGAGACAACTACCGTAACTGGTTGCCAAGATTGTTTGAAGGTGGCATACACCAATGTACAGAGTTGCCTTCCCGATACAGACGGTAGAGCAGTTGATGCCGGTTGTTTCTTGAGATACTCTGCTACTTCTTTCTTCCCAGATAACCAGACTACTAATATCACGCCTTTCTTAGAGAATGGAGGTAATTCAAGAAAGAAGGCCATCATCATTGGAGTAGTGGTTGGAGGTGTAGGTCTTATTTTACTCATTGTTGGAATTTTCATCTATTTCAGACTTTCAAGGAAGCCCAAGACGGTTCGTAGAGGGGACATATTGGGAGCAACCGAGTTGCAAGGTCCCGTGAATTATAAGTATAAAGATCTGAAGTCGGCAACAGCAAATTTTAGTGATGCAAATAAGCTAGGAGAAGGAGGATTCGGGGACGTCTACAAGGCAACTCTAAACAATGGGAAAACAGTTGCAGTAAAGAAACTAGCTATACTCCAATCAGACAGGGCAATGACAAGTTTCGAAAATGAAGTCAAGCTTATAAGTAATGTACATCACCGGAATCTAATTCGCCTTCTTGGATGCTGTAGCAAAGGACCAGAATTACTTCTGATATACGAATACATGGCAAACAAGAGTCTGGACAAATTCTTATTTGGTCAGAAAAGAGGCTCTCTCAACTGGAAGCAACGGAATGATATAATCCTCGGTACAGCCAGGGGTCTAGCCTATCTACATGAGGAATTCCATGTATGTATCATACATAGAGATATAAAAACCAGCAACATTCTCTTGGATGATGGTTTTCAGCCAAGAATTGCAGATTTTGGGTTGGCAAGGCTTTTACCCGAGGATCAAACTCATCTTAGTACCCGATTTGCAGGAACATTAGGATACACAGCACCTGAATATGCAATCCATGGTCAATTGTCAGAAAAGGTGGATACCTATAGCTATGGAGTAGTAGTCCTTGAAATTGTAAGCGGCCAAAAGAGCAGTGAAATCAAATCTGACACTATGGGTGAATTTCTCCTAGAAAAGGCCTGGAAACTCTATGAGAACGGCCAGCACTTGGAGTTGGTCGATGATACATTGGATCCCAACGATTATGAAGCAGAAGATGTGAAAAAGATAGTTGAGATTGCTCTAATGTGCACTCAATCATCACCTGCTCTAAGGCCAACAATGTCTGAAATTGTTGTTTTACTCAAAAACACAGGCTCTTGGGTAAATAGGCCGCTTACTAGGCCTGTGTTTGTTGATTCTGACCATAAAAGAGTACGGGTGGACACGTCAACTTCTACTGGTTCCTCATCATCCAATGCTACCGCATCCATCTCTCAACTTTCAGGTCGCTAA

Protein Analysis

651

Amino Acids

71.07

Weight (kDa)

7.45

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 37 - 130 3.4e-17 Salt stress response/antifungal
Stress-antifung PF01657 148 - 244 6.7e-13 Salt stress response/antifungal
Pkinase PF00069 332 - 599 1.2e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 335 - 600 4.4e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 957, 1139
AarI CACCTGC 1 cut(s) 1774
AasI GACNNNNNNGTC 1 cut(s) 28
AatII GACGTC 1 cut(s) 1035
Acc36I ACCTGC 1 cut(s) 1774
AccB7I CCANNNNNTGG 1 cut(s) 1669
AccI GTMKAC 3 cut(s) 246, 360, 1035
AccII CGCG 1 cut(s) 354
AciI CCGC 9 cut(s) 83, 282, 306, 309, 325, 567, 1581, 1835, 1926
AclWI GGATC 5 cut(s) 466, 513, 1458, 1688, 1701
AcoI YGGCCR 2 cut(s) 1582, 1660
AcsI RAATTY 5 cut(s) 864, 988, 1244, 1340, 1622
AcuI CTGAAG 2 cut(s) 510, 992
AcyI GRCGYC 1 cut(s) 1032
AdeI CACNNNGTG 1 cut(s) 605
AfaI GTAC 6 cut(s) 119, 666, 1149, 1309, 1468, 1878
AfiI CCNNNNNNNGG 2 cut(s) 1636, 1669
AflIII ACRYGT 2 cut(s) 352, 1887
AgsI TTSAA 5 cut(s) 512, 646, 798, 888, 1571
AjiI CACGTC 1 cut(s) 1890
AjnI CCWGG 3 cut(s) 424, 1313, 1640
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 7 cut(s) 187, 416, 485, 1009, 1085, 1135, 1551
AluI AGCT 7 cut(s) 187, 416, 485, 1009, 1085, 1135, 1551
Alw21I GWGCWC 1 cut(s) 1754
Alw26I GTCTC 2 cut(s) 319, 605
Alw44I GTGCAC 1 cut(s) 1750
AlwI GGATC 5 cut(s) 466, 513, 1458, 1688, 1701
AlwNI CAGNNNCTG 2 cut(s) 488, 1502
Ama87I CYCGRG 1 cut(s) 1445
AoxI GGCC 8 cut(s) 225, 807, 1582, 1638, 1660, 1775, 1832, 1844
ApaLI GTGCAC 1 cut(s) 1750
ApeKI GCWGC 7 cut(s) 111, 184, 198, 369, 482, 485, 545
ApoI RAATTY 5 cut(s) 864, 988, 1244, 1340, 1622
ArsI GACNNNNNNTTYG 2 cut(s) 1113, 1145
AseI ATTAAT 1 cut(s) 531
Asp700I GAANNNNTTC 2 cut(s) 495, 1203
AspLEI GCGC 3 cut(s) 184, 220, 350
AspS9I GGNCC 3 cut(s) 172, 944, 1193
AsuHPI GGTGA 3 cut(s) 1145, 1631, 1755
AsuII TTCGAA 1 cut(s) 1119
AvaI CYCGRG 1 cut(s) 1445
AvaII GGWCC 3 cut(s) 172, 944, 1193
BaeGI GKGCMC 1 cut(s) 1754
BamHI GGATCC 1 cut(s) 1693
BanII GRGCYC 1 cut(s) 236
BarI GAAGNNNNNNTAC 4 cut(s) 405, 437, 638, 670
Bbv12I GWGCWC 1 cut(s) 1754
BbvI GCAGC 7 cut(s) 123, 196, 210, 356, 472, 494, 557
BccI CCATC 3 cut(s) 818, 1391, 1940
BceAI ACGGC 5 cut(s) 211, 231, 296, 382, 1675
BciT130I CCWGG 3 cut(s) 426, 1315, 1642
BciVI GTATCC 2 cut(s) 1484, 1534
BcoDI GTCTC 2 cut(s) 319, 605
BfaI CTAG 5 cut(s) 1010, 1082, 1322, 1631, 1842
BfmI CTRYAG 4 cut(s) 411, 480, 1183, 1546
BfuAI ACCTGC 1 cut(s) 1774
BfuI GTATCC 2 cut(s) 1484, 1534
BglII AGATCT 1 cut(s) 967
Bme1390I CCNGG 3 cut(s) 426, 1315, 1642
Bme18I GGWCC 3 cut(s) 172, 944, 1193
BmeT110I CYCGRG 1 cut(s) 1445
BmgBI CACGTC 1 cut(s) 1890
BmgT120I GGNCC 3 cut(s) 172, 944, 1193
BmiI GGNNCC 4 cut(s) 235, 946, 1695, 1906
BmrFI CCNGG 3 cut(s) 426, 1315, 1642
BmsI GCATC 5 cut(s) 557, 694, 988, 1169, 1937
BplI GAGNNNNNCTC 2 cut(s) 1257, 1289
Bpu14I TTCGAA 1 cut(s) 1119
BpuEI CTTGAG 1 cut(s) 739
BsaHI GRCGYC 1 cut(s) 1032
BsaJI CCNNGG 5 cut(s) 228, 1303, 1314, 1446, 1516
BsaWI WCCGGW 2 cut(s) 378, 1155
BsaXI ACNNNNNCTCC 8 cut(s) 260, 263, 290, 293, 813, 843, 1548, 1578
Bsc4I CCNNNNNNNGG 2 cut(s) 1636, 1669
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 5 cut(s) 115, 460, 631, 1283, 1906
Bse3DI GCAATG 1 cut(s) 1113
BseBI CCWGG 3 cut(s) 426, 1315, 1642
BseDI CCNNGG 5 cut(s) 228, 1303, 1314, 1446, 1516
BseGI GGATG 4 cut(s) 1184, 1399, 1913, 1928
BseLI CCNNNNNNNGG 2 cut(s) 1636, 1669
BseMI GCAATG 1 cut(s) 1113
BseNI ACTGG 5 cut(s) 115, 460, 631, 1283, 1906
BseRI GAGGAG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 1754
BseXI GCAGC 7 cut(s) 123, 196, 210, 356, 472, 494, 557
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 1 cut(s) 354
BshFI GGCC 8 cut(s) 227, 809, 1584, 1640, 1662, 1777, 1834, 1846
BsiHKAI GWGCWC 1 cut(s) 1754
BsiHKCI CYCGRG 1 cut(s) 1445
BsiSI CCGG 3 cut(s) 379, 708, 1156
BslFI GGGAC 3 cut(s) 929, 930, 1043
BslI CCNNNNNNNGG 2 cut(s) 1636, 1669
BsmAI GTCTC 2 cut(s) 319, 605
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 3 cut(s) 929, 930, 1043
BsnI GGCC 8 cut(s) 227, 809, 1584, 1640, 1662, 1777, 1834, 1846
BsoBI CYCGRG 1 cut(s) 1445
Bsp119I TTCGAA 1 cut(s) 1119
Bsp1286I GDGCHC 2 cut(s) 236, 1754
Bsp1407I TGTACA 2 cut(s) 664, 1147
Bsp143I GATC 7 cut(s) 42, 87, 458, 505, 967, 1450, 1693
Bsp19I CCATGG 1 cut(s) 1516
BspACI CCGC 9 cut(s) 83, 282, 306, 309, 325, 567, 1581, 1835, 1926
BspANI GGCC 8 cut(s) 227, 809, 1584, 1640, 1662, 1777, 1834, 1846
BspFNI CGCG 1 cut(s) 354
BspLI GGNNCC 4 cut(s) 235, 946, 1695, 1906
BspMAI CTGCAG 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 1774
BspPI GGATC 5 cut(s) 466, 513, 1458, 1688, 1701
BspT104I TTCGAA 1 cut(s) 1119
BsrDI GCAATG 1 cut(s) 1113
BsrFI RCCGGY 1 cut(s) 707
BsrGI TGTACA 2 cut(s) 664, 1147
BsrI ACTGG 5 cut(s) 115, 460, 631, 1283, 1906
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 5 cut(s) 228, 1303, 1314, 1446, 1516
BssMI GATC 7 cut(s) 42, 87, 458, 505, 967, 1450, 1693
BssNI GRCGYC 1 cut(s) 1032
BssT1I CCWWGG 2 cut(s) 228, 1516
Bst2UI CCWGG 3 cut(s) 426, 1315, 1642
Bst4CI ACNGT 8 cut(s) 30, 290, 412, 586, 622, 692, 904, 1066
BstACI GRCGYC 1 cut(s) 1032
BstAPI GCANNNNNTGC 2 cut(s) 605, 704
BstAUI TGTACA 2 cut(s) 664, 1147
BstBI TTCGAA 1 cut(s) 1119
BstC8I GCNNGC 3 cut(s) 352, 465, 1664
BstDEI CTNAG 4 cut(s) 552, 778, 1463, 1772
BstDSI CCRYGG 1 cut(s) 1516
BstENI CCTNNNNNAGG 1 cut(s) 1634
BstF5I GGATG 4 cut(s) 1184, 1399, 1913, 1928
BstFNI CGCG 1 cut(s) 354
BstHHI GCGC 3 cut(s) 184, 220, 350
BstKTI GATC 7 cut(s) 45, 90, 461, 508, 970, 1453, 1696
BstMAI GTCTC 2 cut(s) 319, 605
BstMBI GATC 7 cut(s) 42, 87, 458, 505, 967, 1450, 1693
BstMWI GCNNNNNNNGC 5 cut(s) 224, 605, 704, 1590, 1843
BstNI CCWGG 3 cut(s) 426, 1315, 1642
BstSCI CCNGG 3 cut(s) 424, 1313, 1640
BstSFI CTRYAG 4 cut(s) 411, 480, 1183, 1546
BstSLI GKGCMC 1 cut(s) 1754
BstUI CGCG 1 cut(s) 354
BstV1I GCAGC 7 cut(s) 123, 196, 210, 356, 472, 494, 557
BstX2I RGATCY 3 cut(s) 505, 967, 1693
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 3 cut(s) 505, 967, 1693
BsuI GTATCC 2 cut(s) 1484, 1534
BsuRI GGCC 8 cut(s) 227, 809, 1584, 1640, 1662, 1777, 1834, 1846
BtgI CCRYGG 1 cut(s) 1516
BtrI CACGTC 1 cut(s) 1890
BtsCI GGATG 4 cut(s) 1184, 1399, 1913, 1928
BtsI GCAGTG 3 cut(s) 266, 397, 1600
BtsIMutI CAGTG 4 cut(s) 26, 266, 397, 1600
BveI ACCTGC 1 cut(s) 1774
Cac8I GCNNGC 3 cut(s) 352, 465, 1664
CaiI CAGNNNCTG 2 cut(s) 488, 1502
CfoI GCGC 3 cut(s) 184, 220, 350
Cfr10I RCCGGY 1 cut(s) 707
Cfr13I GGNCC 3 cut(s) 172, 944, 1193
CseI GACGC 2 cut(s) 265, 343
Csp6I GTAC 6 cut(s) 118, 665, 1148, 1308, 1467, 1877
CviAII CATG 5 cut(s) 253, 1222, 1334, 1346, 1517
CviQI GTAC 6 cut(s) 118, 665, 1148, 1308, 1467, 1877
DdeI CTNAG 4 cut(s) 552, 778, 1463, 1772
DpnI GATC 7 cut(s) 44, 89, 460, 507, 969, 1452, 1695
DpnII GATC 7 cut(s) 42, 87, 458, 505, 967, 1450, 1693
DraIII CACNNNGTG 1 cut(s) 605
DrdI GACNNNNNNGTC 1 cut(s) 28
DseDI GACNNNNNNGTC 1 cut(s) 28
EaeI YGGCCR 2 cut(s) 1582, 1660
EciI GGCGGA 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 228, 1516
Eco147I AGGCCT 2 cut(s) 1640, 1846
Eco24I GRGCYC 1 cut(s) 236
Eco47I GGWCC 3 cut(s) 172, 944, 1193
Eco57I CTGAAG 2 cut(s) 510, 992
Eco88I CYCGRG 1 cut(s) 1445
EcoNI CCTNNNNNAGG 1 cut(s) 1634
EcoO109I RGGNCCY 1 cut(s) 944
EcoRI GAATTC 1 cut(s) 1340
EcoRII CCWGG 3 cut(s) 424, 1313, 1640
EcoT14I CCWWGG 2 cut(s) 228, 1516
EcoT38I GRGCYC 1 cut(s) 236
ErhI CCWWGG 2 cut(s) 228, 1516
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 5 cut(s) 256, 1225, 1337, 1349, 1520
FalI AAGNNNNNCTT 2 cut(s) 1119, 1151
FaqI GGGAC 3 cut(s) 929, 930, 1043
FatI CATG 5 cut(s) 252, 1221, 1333, 1345, 1516
FauI CCCGC 1 cut(s) 90
FblI GTMKAC 3 cut(s) 246, 360, 1035
FokI GGATG 4 cut(s) 1191, 1406, 1900, 1915
FriOI GRGCYC 1 cut(s) 236
FspBI CTAG 5 cut(s) 1010, 1082, 1322, 1631, 1842
GlaI GCGC 3 cut(s) 183, 219, 349
HaeIII GGCC 8 cut(s) 227, 809, 1584, 1640, 1662, 1777, 1834, 1846
HapII CCGG 3 cut(s) 379, 708, 1156
HgaI GACGC 2 cut(s) 265, 343
HhaI GCGC 3 cut(s) 184, 220, 350
Hin1I GRCGYC 1 cut(s) 1032
Hin1II CATG 5 cut(s) 256, 1225, 1337, 1349, 1520
Hin6I GCGC 3 cut(s) 182, 218, 348
HinP1I GCGC 3 cut(s) 182, 218, 348
HincII GTYRAC 1 cut(s) 1893
HindII GTYRAC 1 cut(s) 1893
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 6 cut(s) 438, 448, 1023, 1159, 1234, 1859
HpaII CCGG 3 cut(s) 379, 708, 1156
HphI GGTGA 3 cut(s) 1145, 1631, 1755
Hpy166II GTNNAC 6 cut(s) 247, 361, 1036, 1752, 1885, 1893
Hpy188III TCNNGA 4 cut(s) 680, 718, 798, 1238
Hpy8I GTNNAC 6 cut(s) 247, 361, 1036, 1752, 1885, 1893
Hpy99I CGWCG 2 cut(s) 359, 368
HpyAV CCTTC 6 cut(s) 431, 640, 686, 799, 1010, 1181
HpyCH4III ACNGT 8 cut(s) 30, 290, 412, 586, 622, 692, 904, 1066
HpyCH4IV ACGT 2 cut(s) 1032, 1889
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 605, 704, 1590, 1843
HpyF3I CTNAG 4 cut(s) 552, 778, 1463, 1772
HpySE526I ACGT 2 cut(s) 1032, 1889
Hsp92I GRCGYC 1 cut(s) 1032
Hsp92II CATG 5 cut(s) 256, 1225, 1337, 1349, 1520
HspAI GCGC 3 cut(s) 182, 218, 348
Kzo9I GATC 7 cut(s) 42, 87, 458, 505, 967, 1450, 1693
LmnI GCTCC 2 cut(s) 239, 926
Lsp1109I GCAGC 7 cut(s) 123, 196, 210, 356, 472, 494, 557
LweI GCATC 5 cut(s) 557, 694, 988, 1169, 1937
MaeI CTAG 5 cut(s) 1010, 1082, 1322, 1631, 1842
MaeII ACGT 2 cut(s) 1032, 1889
MaeIII GTNAC 1 cut(s) 622
MalI GATC 7 cut(s) 44, 89, 460, 507, 969, 1452, 1695
MboI GATC 7 cut(s) 42, 87, 458, 505, 967, 1450, 1693
MboII GAAGA 6 cut(s) 25, 86, 500, 503, 733, 1727
MfeI CAATTG 1 cut(s) 1523
MflI RGATCY 3 cut(s) 505, 967, 1693
MhlI GDGCHC 2 cut(s) 236, 1754
MluI ACGCGT 1 cut(s) 352
MlyI GAGTC 2 cut(s) 432, 1243
MmeI TCCRAC 4 cut(s) 162, 261, 811, 841
MroXI GAANNNNTTC 2 cut(s) 495, 1203
MseI TTAA 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 3 cut(s) 379, 708, 1156
MspR9I CCNGG 3 cut(s) 426, 1315, 1642
MunI CAATTG 1 cut(s) 1523
MvaI CCWGG 3 cut(s) 426, 1315, 1642
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 5 cut(s) 224, 605, 704, 1590, 1843
NcoI CCATGG 1 cut(s) 1516
NdeII GATC 7 cut(s) 42, 87, 458, 505, 967, 1450, 1693
NlaIII CATG 5 cut(s) 256, 1225, 1337, 1349, 1520
NlaIV GGNNCC 4 cut(s) 235, 946, 1695, 1906
NmeAIII GCCGAG 1 cut(s) 634
NspV TTCGAA 1 cut(s) 1119
PaqCI CACCTGC 1 cut(s) 1774
PceI AGGCCT 2 cut(s) 1640, 1846
PdmI GAANNNNTTC 2 cut(s) 495, 1203
PfeI GAWTC 4 cut(s) 448, 1023, 1159, 1859
PflFI GACNNNGTC 1 cut(s) 242
PflMI CCANNNNNTGG 1 cut(s) 1669
PleI GAGTC 2 cut(s) 432, 1242
PpsI GAGTC 2 cut(s) 432, 1242
PpuMI RGGWCCY 1 cut(s) 944
PshBI ATTAAT 1 cut(s) 531
PsiI TTATAA 2 cut(s) 957, 1139
Psp5II RGGWCCY 1 cut(s) 944
Psp6I CCWGG 3 cut(s) 424, 1313, 1640
PspGI CCWGG 3 cut(s) 424, 1313, 1640
PspN4I GGNNCC 4 cut(s) 235, 946, 1695, 1906
PspPI GGNCC 3 cut(s) 172, 944, 1193
PspPPI RGGWCCY 1 cut(s) 944
PstI CTGCAG 1 cut(s) 484
PstNI CAGNNNCTG 2 cut(s) 488, 1502
PsuI RGATCY 3 cut(s) 505, 967, 1693
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 6 cut(s) 119, 666, 1149, 1309, 1468, 1878
RsaNI GTAC 6 cut(s) 118, 665, 1148, 1308, 1467, 1877
SaqAI TTAA 1 cut(s) 531
Sau3AI GATC 7 cut(s) 42, 87, 458, 505, 967, 1450, 1693
Sau96I GGNCC 3 cut(s) 172, 944, 1193
SchI GAGTC 2 cut(s) 432, 1243
ScrFI CCNGG 3 cut(s) 426, 1315, 1642
SduI GDGCHC 2 cut(s) 236, 1754
SfaNI GCATC 5 cut(s) 557, 694, 988, 1169, 1937
SfcI CTRYAG 4 cut(s) 411, 480, 1183, 1546
SfuI TTCGAA 1 cut(s) 1119
SinI GGWCC 3 cut(s) 172, 944, 1193
SmlI CTYRAG 1 cut(s) 718
SmoI CTYRAG 1 cut(s) 718
SseBI AGGCCT 2 cut(s) 1640, 1846
SsiI CCGC 9 cut(s) 83, 282, 306, 309, 325, 567, 1581, 1835, 1926
SspMI CTAG 5 cut(s) 1010, 1082, 1322, 1631, 1842
StuI AGGCCT 2 cut(s) 1640, 1846
StyD4I CCNGG 3 cut(s) 424, 1313, 1640
StyI CCWWGG 2 cut(s) 228, 1516
TaaI ACNGT 8 cut(s) 30, 290, 412, 586, 622, 692, 904, 1066
TaiI ACGT 2 cut(s) 1035, 1892
TaqI TCGA 2 cut(s) 1119, 1680
TatI WGTACW 3 cut(s) 117, 664, 1147
TauI GCSGC 4 cut(s) 308, 311, 1584, 1837
TfiI GAWTC 4 cut(s) 448, 1023, 1159, 1859
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 4 cut(s) 33, 266, 397, 1600
TseI GCWGC 7 cut(s) 111, 184, 198, 369, 482, 485, 545
TspDTI ATGAA 4 cut(s) 20, 859, 1140, 1722
TspGWI ACGGA 1 cut(s) 1303
TspRI CASTG 4 cut(s) 33, 266, 397, 1600
Tth111I GACNNNGTC 1 cut(s) 242
Van91I CCANNNNNTGG 1 cut(s) 1669
VneI GTGCAC 1 cut(s) 1750
VpaK11BI GGWCC 3 cut(s) 172, 944, 1193
VspI ATTAAT 1 cut(s) 531
XagI CCTNNNNNAGG 1 cut(s) 1634
XapI RAATTY 5 cut(s) 864, 988, 1244, 1340, 1622
XmiI GTMKAC 3 cut(s) 246, 360, 1035
XmnI GAANNNNTTC 2 cut(s) 495, 1203
XspI CTAG 5 cut(s) 1010, 1082, 1322, 1631, 1842
ZraI GACGTC 1 cut(s) 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.