Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0005518

Physical Location & Seq

Genomic Coordinates Forward (+)

33437846 .. 33451253

Gene Structure 13408 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G025250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGGATCTTCAAGTTGCTACGCCCAAGATTAATGGCTTCTTTGCAGCATCTAAGGAGGTAGTTAGCGGTAGTGGAAACACAACAGTCTATGGTGTGGCACAATGTGCTGAGACAACTACCGTAACTGGTTGCCAAGATTGTTTGAAGGTGGCATACACCAATGTACAGAGTTGCCTTCCCGATACAGATGGTAGGGCAGTTGATGCCGGTTGTTTCTTGAGGTACTCTGCTACTTCTTTCTTCCCAGATAACCAGACTACTAATATCAAGCCTTTCTTAGAAAAAGGAGGCAATTCAAGAAAGAAGGCCATCATCATTGGAGTAGTGGTTGGAGGTGTAGGTCTTATTTTACTCATTGTTGGAGTTTTGATCTATTTCAGACTTTCAAGGAAGCCCAAGACGGTTCGTAGAGGAGACATATTGGGAGCAACCGAGTTGCAAGGTCCAGTGAATTATAAGTATAAAGATCTGAAGTCGGCAACAGCAAATTTTAGTGATGCAAATAAGCTAGGAGAAGGAGGATTCGGGGACGTCTACAAGGCAACTCTAAACAATGGGAAAACAGTTGCAGTAAAGAAACTAGCTATACTCCAATCAGACAGGGCAATGACAAGTTTCGAAAATGAAGTCAAGCTTATAAGTAATGTACATCACCGGAATCTAATTCGCCTTCTTGGATGTTGTAGCAAAGGACCAGAATTACTTCTGATATATGAATACATGGCAAACAACAGTCTGGACAAATTCTTATTTGGTGAGAAAAGAGGCTTTCTAAACTGGAAGCAAAGGAATGATATAATCGTTGGTACAGCTAGGGGCCTAGCCTATCTACATGAGGAATTCCATGTATGTATCATACATAGAGATATAAAAACCAGCAACATTCTCTTGGATGATGGTTTTCAGCCAAGAATTGCAGATTTTGGGTTGGCAAGGCTTTTACCTGAGGATCAAACTCATCTTAGTACCCGATTTGCAGGAACATTAGGATACACAGCACCTGAATATGCAATCCATGGTCAATTGTCAGAAAAGGTGGATACCTATAGCTATGGAGTAGTAGTCCTTGAAATTGTAAGCGGCCAAAAGAGCAGTGAAATCAAATCTGATACTATGGGTGAATTTCTCCTAGAAAAGGCCTGGAAACTCTATGAGAACGGCCAGCACTTGGAGTTGGTCGATGAAACATTGGATCCCAACGATTATGAAGCAGAAGATGTGAAAAAGATAGTTGAGATTGCTCTAATGTGCACTCAATCATCACCTGCTCTAAGGCCAACAATGTCTGAAATTGTTGTTTTACTCAAAAGCACAGGCTCTTGGGTAAATAGGCCGCTTACTAGGCCTGTGTTTGTTGATTCTGACCATAAAAGAGTACGGGTGGACACGTCAACTTCTACTGCTTCCTCATCATCCAATGCTACCGCATCCATCTCTCAACTTTCAGGTCGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

484

Amino Acids

53.09

Weight (kDa)

7.97

Isoelectric Point (pI)

28.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	12 - 77	4.4e-10	Salt stress response/antifungal
Pkinase	PF00069	165 - 432	9.2e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	168 - 433	3e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005518)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g27612 FvH4_1g27613 FvH4_2g29620 FvH4_2g29620 FvH4_2g29620 FvH4_2g29620
malus_domestica	MD09G1281900.v1.1
prunus_persica	Prupe.3G012100_v2.0.a1
pyrus_communis	pycom09g18970 pycom09g18990
rosa_chinensis	RchiOBHm_Chr3g0487811 RchiOBHm_Chr3g0487871 RchiOBHm_Chr3g0487881
rosa_laevigata	RLG00000022971 RLG00000028062
rosa_multiflora	Rmu_sc0004448.1_g000005 Rmu_sc0008363.1_g000023
rosa_roxburghii	Rroxscaffold_3G00220850 Rroxscaffold_3G00252340 Rroxscaffold_5G00352940 Rroxscaffold_6G00394450 Rroxscaffold_6G00394460
rosa_rugosa	Rorug03G0236900 Rorug03G0237000
rosa_samantha	Rh3AG284800 Rh3BG321600 Rh3CG320300 Rh3DG318800
rosa_wichuraiana	Rw3G025250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	456, 638
AarI	CACCTGC	1 cut(s)	1273
AatII	GACGTC	1 cut(s)	534
Acc36I	ACCTGC	1 cut(s)	1273
AccB7I	CCANNNNNTGG	1 cut(s)	1168
AccI	GTMKAC	1 cut(s)	534
AciI	CCGC	4 cut(s)	66, 1080, 1334, 1425
AclWI	GGATC	4 cut(s)	12, 957, 1187, 1200
AcoI	YGGCCR	2 cut(s)	1081, 1159
AcsI	RAATTY	4 cut(s)	487, 743, 839, 1121
AcuI	CTGAAG	1 cut(s)	491
AcyI	GRCGYC	1 cut(s)	531
AdeI	CACNNNGTG	1 cut(s)	104
AfaI	GTAC	6 cut(s)	165, 224, 648, 808, 967, 1377
AfiI	CCNNNNNNNGG	2 cut(s)	1135, 1168
AflIII	ACRYGT	1 cut(s)	1386
AgsI	TTSAA	5 cut(s)	11, 145, 297, 387, 1070
AjiI	CACGTC	1 cut(s)	1389
AjnI	CCWGG	1 cut(s)	1139
AluBI	AGCT	5 cut(s)	508, 584, 634, 812, 1050
AluI	AGCT	5 cut(s)	508, 584, 634, 812, 1050
Alw21I	GWGCWC	1 cut(s)	1253
Alw26I	GTCTC	2 cut(s)	104, 408
Alw44I	GTGCAC	1 cut(s)	1249
AlwI	GGATC	4 cut(s)	12, 957, 1187, 1200
AlwNI	CAGNNNCTG	1 cut(s)	1001
AoxI	GGCC	8 cut(s)	306, 817, 1081, 1137, 1159, 1274, 1331, 1343
ApaLI	GTGCAC	1 cut(s)	1249
ApeKI	GCWGC	1 cut(s)	44
ApoI	RAATTY	4 cut(s)	487, 743, 839, 1121
ArsI	GACNNNNNNTTYG	2 cut(s)	612, 644
AseI	ATTAAT	1 cut(s)	30
Asp700I	GAANNNNTTC	1 cut(s)	702
AspS9I	GGNCC	3 cut(s)	443, 692, 817
AsuHPI	GGTGA	4 cut(s)	644, 767, 1130, 1254
AsuII	TTCGAA	1 cut(s)	618
AvaII	GGWCC	2 cut(s)	443, 692
AxyI	CCTNAGG	1 cut(s)	945
BaeGI	GKGCMC	1 cut(s)	1253
BamHI	GGATCC	1 cut(s)	1192
BarI	GAAGNNNNNNTAC	2 cut(s)	137, 169
Bbv12I	GWGCWC	1 cut(s)	1253
BbvI	GCAGC	1 cut(s)	56
BccI	CCATC	4 cut(s)	182, 317, 890, 1439
BceAI	ACGGC	1 cut(s)	1174
BciT130I	CCWGG	1 cut(s)	1141
BciVI	GTATCC	2 cut(s)	983, 1033
BcoDI	GTCTC	2 cut(s)	104, 408
BfaI	CTAG	6 cut(s)	509, 581, 813, 821, 1130, 1341
BfmI	CTRYAG	1 cut(s)	1045
BfuAI	ACCTGC	1 cut(s)	1273
BfuI	GTATCC	2 cut(s)	983, 1033
BglII	AGATCT	1 cut(s)	466
BisI	GCNGC	3 cut(s)	45, 1081, 1334
BlsI	GCNGC	3 cut(s)	46, 1082, 1335
Bme1390I	CCNGG	1 cut(s)	1141
Bme18I	GGWCC	2 cut(s)	443, 692
BmgBI	CACGTC	1 cut(s)	1389
BmgT120I	GGNCC	3 cut(s)	443, 692, 817
BmiI	GGNNCC	2 cut(s)	818, 1194
BmrFI	CCNGG	1 cut(s)	1141
BmsI	GCATC	4 cut(s)	56, 193, 487, 1436
Bpu14I	TTCGAA	1 cut(s)	618
BpuEI	CTTGAG	1 cut(s)	238
BsaHI	GRCGYC	1 cut(s)	531
BsaJI	CCNNGG	1 cut(s)	1015
BsaWI	WCCGGW	1 cut(s)	654
BsaXI	ACNNNNNCTCC	4 cut(s)	312, 342, 1047, 1077
Bsc4I	CCNNNNNNNGG	2 cut(s)	1135, 1168
Bse118I	RCCGGY	1 cut(s)	206
Bse1I	ACTGG	3 cut(s)	130, 446, 782
Bse21I	CCTNAGG	1 cut(s)	945
Bse3DI	GCAATG	1 cut(s)	612
BseBI	CCWGG	1 cut(s)	1141
BseDI	CCNNGG	1 cut(s)	1015
BseGI	GGATG	4 cut(s)	683, 898, 1412, 1427
BseLI	CCNNNNNNNGG	2 cut(s)	1135, 1168
BseMI	GCAATG	1 cut(s)	612
BseMII	CTCAG	2 cut(s)	99, 936
BseNI	ACTGG	3 cut(s)	130, 446, 782
BseRI	GAGGAG	1 cut(s)	426
BseSI	GKGCMC	1 cut(s)	1253
BseXI	GCAGC	1 cut(s)	56
BshFI	GGCC	8 cut(s)	308, 819, 1083, 1139, 1161, 1276, 1333, 1345
BsiHKAI	GWGCWC	1 cut(s)	1253
BsiSI	CCGG	2 cut(s)	207, 655
BslFI	GGGAC	1 cut(s)	542
BslI	CCNNNNNNNGG	2 cut(s)	1135, 1168
BsmAI	GTCTC	2 cut(s)	104, 408
BsmFI	GGGAC	1 cut(s)	542
BsnI	GGCC	8 cut(s)	308, 819, 1083, 1139, 1161, 1276, 1333, 1345
Bsp119I	TTCGAA	1 cut(s)	618
Bsp1286I	GDGCHC	1 cut(s)	1253
Bsp1407I	TGTACA	2 cut(s)	163, 646
Bsp143I	GATC	5 cut(s)	4, 369, 466, 949, 1192
Bsp19I	CCATGG	1 cut(s)	1015
BspACI	CCGC	4 cut(s)	66, 1080, 1334, 1425
BspANI	GGCC	8 cut(s)	308, 819, 1083, 1139, 1161, 1276, 1333, 1345
BspCNI	CTCAG	2 cut(s)	100, 937
BspLI	GGNNCC	2 cut(s)	818, 1194
BspMI	ACCTGC	1 cut(s)	1273
BspPI	GGATC	4 cut(s)	12, 957, 1187, 1200
BspT104I	TTCGAA	1 cut(s)	618
BsrDI	GCAATG	1 cut(s)	612
BsrFI	RCCGGY	1 cut(s)	206
BsrGI	TGTACA	2 cut(s)	163, 646
BsrI	ACTGG	3 cut(s)	130, 446, 782
BssAI	RCCGGY	1 cut(s)	206
BssECI	CCNNGG	1 cut(s)	1015
BssMI	GATC	5 cut(s)	4, 369, 466, 949, 1192
BssNI	GRCGYC	1 cut(s)	531
BssT1I	CCWWGG	1 cut(s)	1015
Bst2UI	CCWGG	1 cut(s)	1141
Bst4CI	ACNGT	5 cut(s)	85, 121, 403, 565, 734
BstACI	GRCGYC	1 cut(s)	531
BstAPI	GCANNNNNTGC	2 cut(s)	104, 203
BstAUI	TGTACA	2 cut(s)	163, 646
BstBI	TTCGAA	1 cut(s)	618
BstC8I	GCNNGC	1 cut(s)	1163
BstDEI	CTNAG	6 cut(s)	51, 108, 277, 945, 962, 1271
BstDSI	CCRYGG	1 cut(s)	1015
BstENI	CCTNNNNNAGG	1 cut(s)	1133
BstF5I	GGATG	4 cut(s)	683, 898, 1412, 1427
BstKTI	GATC	5 cut(s)	7, 372, 469, 952, 1195
BstMAI	GTCTC	2 cut(s)	104, 408
BstMBI	GATC	5 cut(s)	4, 369, 466, 949, 1192
BstMWI	GCNNNNNNNGC	4 cut(s)	104, 203, 1089, 1342
BstNI	CCWGG	1 cut(s)	1141
BstSCI	CCNGG	1 cut(s)	1139
BstSFI	CTRYAG	1 cut(s)	1045
BstSLI	GKGCMC	1 cut(s)	1253
BstV1I	GCAGC	1 cut(s)	56
BstX2I	RGATCY	3 cut(s)	4, 466, 1192
BstYI	RGATCY	3 cut(s)	4, 466, 1192
Bsu36I	CCTNAGG	1 cut(s)	945
BsuI	GTATCC	2 cut(s)	983, 1033
BsuRI	GGCC	8 cut(s)	308, 819, 1083, 1139, 1161, 1276, 1333, 1345
BtgI	CCRYGG	1 cut(s)	1015
BtrI	CACGTC	1 cut(s)	1389
BtsCI	GGATG	4 cut(s)	683, 898, 1412, 1427
BtsI	GCAGTG	1 cut(s)	1099
BtsIMutI	CAGTG	2 cut(s)	453, 1099
BveI	ACCTGC	1 cut(s)	1273
Cac8I	GCNNGC	1 cut(s)	1163
CaiI	CAGNNNCTG	1 cut(s)	1001
Cfr10I	RCCGGY	1 cut(s)	206
Cfr13I	GGNCC	3 cut(s)	443, 692, 817
Csp6I	GTAC	6 cut(s)	164, 223, 647, 807, 966, 1376
CviAII	CATG	4 cut(s)	721, 833, 845, 1016
CviQI	GTAC	6 cut(s)	164, 223, 647, 807, 966, 1376
DdeI	CTNAG	6 cut(s)	51, 108, 277, 945, 962, 1271
DpnI	GATC	5 cut(s)	6, 371, 468, 951, 1194
DpnII	GATC	5 cut(s)	4, 369, 466, 949, 1192
DraIII	CACNNNGTG	1 cut(s)	104
EaeI	YGGCCR	2 cut(s)	1081, 1159
Eco130I	CCWWGG	1 cut(s)	1015
Eco147I	AGGCCT	2 cut(s)	1139, 1345
Eco47I	GGWCC	2 cut(s)	443, 692
Eco57I	CTGAAG	1 cut(s)	491
Eco81I	CCTNAGG	1 cut(s)	945
EcoNI	CCTNNNNNAGG	1 cut(s)	1133
EcoO109I	RGGNCCY	1 cut(s)	817
EcoRI	GAATTC	1 cut(s)	839
EcoRII	CCWGG	1 cut(s)	1139
EcoT14I	CCWWGG	1 cut(s)	1015
ErhI	CCWWGG	1 cut(s)	1015
FaeI	CATG	4 cut(s)	724, 836, 848, 1019
FalI	AAGNNNNNCTT	4 cut(s)	260, 292, 618, 650
FaqI	GGGAC	1 cut(s)	542
FatI	CATG	4 cut(s)	720, 832, 844, 1015
FblI	GTMKAC	1 cut(s)	534
Fnu4HI	GCNGC	3 cut(s)	45, 1081, 1334
FokI	GGATG	4 cut(s)	690, 905, 1399, 1414
Fsp4HI	GCNGC	3 cut(s)	45, 1081, 1334
FspBI	CTAG	6 cut(s)	509, 581, 813, 821, 1130, 1341
GluI	GCNGC	3 cut(s)	45, 1081, 1334
HaeIII	GGCC	8 cut(s)	308, 819, 1083, 1139, 1161, 1276, 1333, 1345
HapII	CCGG	2 cut(s)	207, 655
Hin1I	GRCGYC	1 cut(s)	531
Hin1II	CATG	4 cut(s)	724, 836, 848, 1019
HincII	GTYRAC	1 cut(s)	1392
HindII	GTYRAC	1 cut(s)	1392
HindIII	AAGCTT	1 cut(s)	632
HinfI	GANTC	3 cut(s)	522, 658, 1358
HpaII	CCGG	2 cut(s)	207, 655
HphI	GGTGA	4 cut(s)	644, 767, 1130, 1254
Hpy166II	GTNNAC	4 cut(s)	535, 1251, 1384, 1392
Hpy188I	TCNGA	8 cut(s)	380, 471, 598, 708, 1030, 1108, 1288, 1363
Hpy188III	TCNNGA	4 cut(s)	179, 217, 297, 737
Hpy8I	GTNNAC	4 cut(s)	535, 1251, 1384, 1392
HpyAV	CCTTC	5 cut(s)	139, 185, 298, 509, 680
HpyCH4III	ACNGT	5 cut(s)	85, 121, 403, 565, 734
HpyCH4IV	ACGT	2 cut(s)	531, 1388
HpyCH4V	TGCA	8 cut(s)	44, 439, 500, 569, 917, 977, 1010, 1251
HpyF10VI	GCNNNNNNNGC	4 cut(s)	104, 203, 1089, 1342
HpyF3I	CTNAG	6 cut(s)	51, 108, 277, 945, 962, 1271
HpySE526I	ACGT	2 cut(s)	531, 1388
Hsp92I	GRCGYC	1 cut(s)	531
Hsp92II	CATG	4 cut(s)	724, 836, 848, 1019
Kzo9I	GATC	5 cut(s)	4, 369, 466, 949, 1192
LmnI	GCTCC	1 cut(s)	425
Lsp1109I	GCAGC	1 cut(s)	56
LweI	GCATC	4 cut(s)	56, 193, 487, 1436
MaeI	CTAG	6 cut(s)	509, 581, 813, 821, 1130, 1341
MaeII	ACGT	2 cut(s)	531, 1388
MaeIII	GTNAC	1 cut(s)	121
MalI	GATC	5 cut(s)	6, 371, 468, 951, 1194
MboI	GATC	5 cut(s)	4, 369, 466, 949, 1192
MboII	GAAGA	2 cut(s)	232, 1226
MfeI	CAATTG	1 cut(s)	1022
MflI	RGATCY	3 cut(s)	4, 466, 1192
MhlI	GDGCHC	1 cut(s)	1253
MmeI	TCCRAC	2 cut(s)	310, 340
MroXI	GAANNNNTTC	1 cut(s)	702
MseI	TTAA	1 cut(s)	30
MspI	CCGG	2 cut(s)	207, 655
MspR9I	CCNGG	1 cut(s)	1141
MunI	CAATTG	1 cut(s)	1022
MvaI	CCWGG	1 cut(s)	1141
MwoI	GCNNNNNNNGC	4 cut(s)	104, 203, 1089, 1342
NcoI	CCATGG	1 cut(s)	1015
NdeII	GATC	5 cut(s)	4, 369, 466, 949, 1192
NlaIII	CATG	4 cut(s)	724, 836, 848, 1019
NlaIV	GGNNCC	2 cut(s)	818, 1194
NspV	TTCGAA	1 cut(s)	618
PaqCI	CACCTGC	1 cut(s)	1273
PceI	AGGCCT	2 cut(s)	1139, 1345
PdmI	GAANNNNTTC	1 cut(s)	702
PfeI	GAWTC	3 cut(s)	522, 658, 1358
PflMI	CCANNNNNTGG	1 cut(s)	1168
PkrI	GCNGC	3 cut(s)	46, 1082, 1335
PshBI	ATTAAT	1 cut(s)	30
PsiI	TTATAA	2 cut(s)	456, 638
Psp6I	CCWGG	1 cut(s)	1139
PspGI	CCWGG	1 cut(s)	1139
PspN4I	GGNNCC	2 cut(s)	818, 1194
PspPI	GGNCC	3 cut(s)	443, 692, 817
PstNI	CAGNNNCTG	1 cut(s)	1001
PsuI	RGATCY	3 cut(s)	4, 466, 1192
RsaI	GTAC	6 cut(s)	165, 224, 648, 808, 967, 1377
RsaNI	GTAC	6 cut(s)	164, 223, 647, 807, 966, 1376
SaqAI	TTAA	1 cut(s)	30
SatI	GCNGC	3 cut(s)	45, 1081, 1334
Sau3AI	GATC	5 cut(s)	4, 369, 466, 949, 1192
Sau96I	GGNCC	3 cut(s)	443, 692, 817
ScrFI	CCNGG	1 cut(s)	1141
SduI	GDGCHC	1 cut(s)	1253
SfaNI	GCATC	4 cut(s)	56, 193, 487, 1436
SfcI	CTRYAG	1 cut(s)	1045
SfuI	TTCGAA	1 cut(s)	618
SinI	GGWCC	2 cut(s)	443, 692
SmlI	CTYRAG	1 cut(s)	217
SmoI	CTYRAG	1 cut(s)	217
SseBI	AGGCCT	2 cut(s)	1139, 1345
SsiI	CCGC	4 cut(s)	66, 1080, 1334, 1425
SspMI	CTAG	6 cut(s)	509, 581, 813, 821, 1130, 1341
StuI	AGGCCT	2 cut(s)	1139, 1345
StyD4I	CCNGG	1 cut(s)	1139
StyI	CCWWGG	1 cut(s)	1015
TaaI	ACNGT	5 cut(s)	85, 121, 403, 565, 734
TaiI	ACGT	2 cut(s)	534, 1391
TaqI	TCGA	2 cut(s)	618, 1179
TatI	WGTACW	2 cut(s)	163, 646
TauI	GCSGC	2 cut(s)	1083, 1336
TfiI	GAWTC	3 cut(s)	522, 658, 1358
Tru1I	TTAA	1 cut(s)	30
Tru9I	TTAA	1 cut(s)	30
TscAI	CASTG	2 cut(s)	453, 1099
TseI	GCWGC	1 cut(s)	44
TspDTI	ATGAA	4 cut(s)	639, 729, 1197, 1221
TspRI	CASTG	2 cut(s)	453, 1099
Van91I	CCANNNNNTGG	1 cut(s)	1168
VneI	GTGCAC	1 cut(s)	1249
VpaK11BI	GGWCC	2 cut(s)	443, 692
VspI	ATTAAT	1 cut(s)	30
XagI	CCTNNNNNAGG	1 cut(s)	1133
XapI	RAATTY	4 cut(s)	487, 743, 839, 1121
XmiI	GTMKAC	1 cut(s)	534
XmnI	GAANNNNTTC	1 cut(s)	702
XspI	CTAG	6 cut(s)	509, 581, 813, 821, 1130, 1341
ZraI	GACGTC	1 cut(s)	532

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.