Rroxscaffold_6G00394460

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005518
Physical Location & Seq
Reverse (-)
15130109 .. 15136935
6827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394460.1

Sequence Viewer

Length: 1356 bp
ATGATGAATAAGAAGATATTGGATACACTGTCAATAATTACGATCAGCCAGATATGGTGGTGGGCTTGTGTGAGTGCAGACCCGCAGATCAATCTTCTGAACGAGGGTTGCAGCCAGTACAACGCAACCAGTCTGTCCAACTTCTATACCAATCTCAACGCCACTTTCTTGGACCTCAAAGAGCAGCTACTCAACGGCAGCAAACACTTTGCAACGGCGCAACAGGCCAAGGGCTCCGACCCCGTCTACGCCATGACGCAGTGTAGGAACTACCTCTCCTCCGCCGACTGTCTATCTTGCTTTGCCGCCGCCGTCTCCCAAATCCGCAACTGCTCTGCCTCCAATGGCGCACGCGTCGTCTACGACGGCTGCTTCCTCCGGTATGAGAGCAGTGGTTTTTTTGACCAGACTGTAGCTACCCTTCCTGGTAATGTAGGACTCTGTGGGAATCAAACTGGATCGCCTGCTACAACCTTTACTGCAGCTGCTGAAGAACTTCTAATGGATCTTCAAGTTGCTACGCCCAAGATTAATGGCTTCTTTGCAGCATCTAAGGAGGTAGTTAGCGGTAGTGGAAACACAACAGTCTATGGGTGTGGCACAATGTGCCGCCGAGACAACTACCGTAACAGACTTTCAAGGAAGCCCAAGACGGTTCGTAGAGGGGACATATTGGGAGCAACCGAGTTGCAAGGTCCAGTGAATTATAAGTATAAAGATCTGAAGTCGGCAACAGCAAATTTTAGTGATGCAAATAAGCTAGGGAGAAGGAGGATTCGGGGACGTCTACAAGTACTTTCGTTTTACAATTACATCCTTGCACAGGCAACTCTAAACAATGGGAAAACAGTTGCAGTAAAGAAACTAGCTATACTCCAATCAGACAGGGCAATGACAAGTTTCGAAAATGAAGTCAAGCTTATAAGTTCTTTCTCTTCTTCTTCTTCTTTTTTCCATGCAAATAGAGGATACACAGCACCTGAATATGCAATCCATGGTCAATTGTCAGAAAAGGTGGATACCTATAGCTATGGAGTAGCCTGGAAACTCTATGAGAACGGCCAGCACTTGGGGTTGGTCGATGAAACATTGGATCTCAACGATTATGAAGCAGAAGATGTGAAAAAGATAGTTGAGATTGCTCTAATGTGCACTCAATCATCACCTGCTCTAAGGCCAACAATGTCTGAAATTGTTGTTTTACTCAAAAGCACAGGCTCTTGGGTAAATAGGCCGCTTACTAGGCCTGTGTTTGTTGATTCTGACCATAAAAGAGTACGGGTGGACACGTCAACTTCTACTGGTTCCTCATCATCCAATGCTACCGCATCCATCTCTCAACTTTCAGGTCGCTAA

Protein Analysis

451

Amino Acids

49.43

Weight (kDa)

8.91

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 37 - 130 1.4e-17 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 708, 923
AarI CACCTGC 1 cut(s) 1174
AatII GACGTC 1 cut(s) 787
Acc36I ACCTGC 1 cut(s) 1174
AccB7I CCANNNNNTGG 1 cut(s) 1069
AccI GTMKAC 3 cut(s) 246, 360, 787
AccII CGCG 1 cut(s) 354
AciI CCGC 9 cut(s) 83, 282, 306, 309, 325, 567, 610, 1235, 1326
AclWI GGATC 3 cut(s) 466, 513, 1101
AcoI YGGCCR 1 cut(s) 1060
AcsI RAATTY 1 cut(s) 739
AcuI CTGAAG 2 cut(s) 510, 743
AcyI GRCGYC 1 cut(s) 784
AdeI CACNNNGTG 1 cut(s) 606
AfaI GTAC 3 cut(s) 119, 795, 1278
AfiI CCNNNNNNNGG 2 cut(s) 823, 1069
AflIII ACRYGT 2 cut(s) 352, 1287
AgsI TTSAA 2 cut(s) 512, 639
AjiI CACGTC 1 cut(s) 1290
AjnI CCWGG 2 cut(s) 424, 1040
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 7 cut(s) 187, 416, 485, 760, 869, 919, 1029
AluI AGCT 7 cut(s) 187, 416, 485, 760, 869, 919, 1029
Alw21I GWGCWC 1 cut(s) 1154
Alw26I GTCTC 2 cut(s) 319, 609
Alw44I GTGCAC 1 cut(s) 1150
AlwI GGATC 3 cut(s) 466, 513, 1101
AlwNI CAGNNNCTG 2 cut(s) 488, 980
AoxI GGCC 5 cut(s) 225, 1060, 1175, 1232, 1244
ApaLI GTGCAC 1 cut(s) 1150
ApeKI GCWGC 7 cut(s) 111, 184, 198, 369, 482, 485, 545
ApoI RAATTY 1 cut(s) 739
ArsI GACNNNNNNTTYG 2 cut(s) 897, 929
AseI ATTAAT 1 cut(s) 531
Asp700I GAANNNNTTC 1 cut(s) 495
AspLEI GCGC 2 cut(s) 220, 350
AspS9I GGNCC 2 cut(s) 172, 695
AsuHPI GGTGA 1 cut(s) 1155
AsuII TTCGAA 1 cut(s) 903
AvaII GGWCC 2 cut(s) 172, 695
BaeGI GKGCMC 1 cut(s) 1154
BanII GRGCYC 1 cut(s) 236
BarI GAAGNNNNNNTAC 2 cut(s) 405, 437
Bbv12I GWGCWC 1 cut(s) 1154
BbvI GCAGC 7 cut(s) 123, 196, 210, 356, 472, 494, 557
BccI CCATC 1 cut(s) 1340
BceAI ACGGC 5 cut(s) 211, 231, 296, 382, 1075
BciT130I CCWGG 2 cut(s) 426, 1042
BciVI GTATCC 3 cut(s) 16, 962, 1012
BcoDI GTCTC 2 cut(s) 319, 609
BfaI CTAG 3 cut(s) 761, 866, 1242
BfmI CTRYAG 3 cut(s) 411, 480, 1024
BfuAI ACCTGC 1 cut(s) 1174
BfuI GTATCC 3 cut(s) 16, 962, 1012
BglII AGATCT 1 cut(s) 718
BmcAI AGTACT 1 cut(s) 795
Bme1390I CCNGG 2 cut(s) 426, 1042
Bme18I GGWCC 2 cut(s) 172, 695
BmgBI CACGTC 1 cut(s) 1290
BmgT120I GGNCC 2 cut(s) 172, 695
BmiI GGNNCC 2 cut(s) 235, 1306
BmrFI CCNGG 2 cut(s) 426, 1042
BmsI GCATC 3 cut(s) 557, 739, 1337
Bpu14I TTCGAA 1 cut(s) 903
BsaHI GRCGYC 1 cut(s) 784
BsaJI CCNNGG 2 cut(s) 228, 994
BsaWI WCCGGW 1 cut(s) 378
BsaXI ACNNNNNCTCC 4 cut(s) 260, 263, 290, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 823, 1069
Bse1I ACTGG 5 cut(s) 115, 129, 460, 698, 1306
Bse3DI GCAATG 1 cut(s) 897
BseBI CCWGG 2 cut(s) 426, 1042
BseDI CCNNGG 2 cut(s) 228, 994
BseGI GGATG 3 cut(s) 813, 1313, 1328
BseLI CCNNNNNNNGG 2 cut(s) 823, 1069
BseMI GCAATG 1 cut(s) 897
BseNI ACTGG 5 cut(s) 115, 129, 460, 698, 1306
BseRI GAGGAG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 1154
BseXI GCAGC 7 cut(s) 123, 196, 210, 356, 472, 494, 557
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 1 cut(s) 354
BshFI GGCC 5 cut(s) 227, 1062, 1177, 1234, 1246
BsiHKAI GWGCWC 1 cut(s) 1154
BsiSI CCGG 1 cut(s) 379
BslFI GGGAC 2 cut(s) 680, 795
BslI CCNNNNNNNGG 2 cut(s) 823, 1069
BsmAI GTCTC 2 cut(s) 319, 609
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 2 cut(s) 680, 795
BsnI GGCC 5 cut(s) 227, 1062, 1177, 1234, 1246
Bsp119I TTCGAA 1 cut(s) 903
Bsp1286I GDGCHC 2 cut(s) 236, 1154
Bsp143I GATC 6 cut(s) 42, 87, 458, 505, 718, 1093
Bsp19I CCATGG 1 cut(s) 994
BspACI CCGC 9 cut(s) 83, 282, 306, 309, 325, 567, 610, 1235, 1326
BspANI GGCC 5 cut(s) 227, 1062, 1177, 1234, 1246
BspFNI CGCG 1 cut(s) 354
BspLI GGNNCC 2 cut(s) 235, 1306
BspMAI CTGCAG 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 1174
BspPI GGATC 3 cut(s) 466, 513, 1101
BspT104I TTCGAA 1 cut(s) 903
BsrDI GCAATG 1 cut(s) 897
BsrI ACTGG 5 cut(s) 115, 129, 460, 698, 1306
BssECI CCNNGG 2 cut(s) 228, 994
BssMI GATC 6 cut(s) 42, 87, 458, 505, 718, 1093
BssNI GRCGYC 1 cut(s) 784
BssT1I CCWWGG 2 cut(s) 228, 994
Bst2UI CCWGG 2 cut(s) 426, 1042
Bst4CI ACNGT 7 cut(s) 30, 290, 412, 586, 626, 655, 850
Bst6I CTCTTC 1 cut(s) 940
BstACI GRCGYC 1 cut(s) 784
BstAPI GCANNNNNTGC 1 cut(s) 606
BstBI TTCGAA 1 cut(s) 903
BstC8I GCNNGC 3 cut(s) 352, 465, 1064
BstDEI CTNAG 2 cut(s) 552, 1172
BstDSI CCRYGG 1 cut(s) 994
BstENI CCTNNNNNAGG 1 cut(s) 821
BstF5I GGATG 3 cut(s) 813, 1313, 1328
BstFNI CGCG 1 cut(s) 354
BstHHI GCGC 2 cut(s) 220, 350
BstKTI GATC 6 cut(s) 45, 90, 461, 508, 721, 1096
BstMAI GTCTC 2 cut(s) 319, 609
BstMBI GATC 6 cut(s) 42, 87, 458, 505, 718, 1093
BstMWI GCNNNNNNNGC 3 cut(s) 224, 606, 1243
BstNI CCWGG 2 cut(s) 426, 1042
BstSCI CCNGG 2 cut(s) 424, 1040
BstSFI CTRYAG 3 cut(s) 411, 480, 1024
BstSLI GKGCMC 1 cut(s) 1154
BstUI CGCG 1 cut(s) 354
BstV1I GCAGC 7 cut(s) 123, 196, 210, 356, 472, 494, 557
BstX2I RGATCY 3 cut(s) 505, 718, 1093
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 3 cut(s) 505, 718, 1093
BsuI GTATCC 3 cut(s) 16, 962, 1012
BsuRI GGCC 5 cut(s) 227, 1062, 1177, 1234, 1246
BtgI CCRYGG 1 cut(s) 994
BtrI CACGTC 1 cut(s) 1290
BtsCI GGATG 3 cut(s) 813, 1313, 1328
BtsI GCAGTG 2 cut(s) 266, 397
BtsIMutI CAGTG 4 cut(s) 26, 266, 397, 705
BveI ACCTGC 1 cut(s) 1174
Cac8I GCNNGC 3 cut(s) 352, 465, 1064
CaiI CAGNNNCTG 2 cut(s) 488, 980
CfoI GCGC 2 cut(s) 220, 350
Cfr13I GGNCC 2 cut(s) 172, 695
CseI GACGC 2 cut(s) 265, 343
Csp6I GTAC 3 cut(s) 118, 794, 1277
CviAII CATG 3 cut(s) 253, 956, 995
CviQI GTAC 3 cut(s) 118, 794, 1277
DdeI CTNAG 2 cut(s) 552, 1172
DpnI GATC 6 cut(s) 44, 89, 460, 507, 720, 1095
DpnII GATC 6 cut(s) 42, 87, 458, 505, 718, 1093
DraIII CACNNNGTG 1 cut(s) 606
EaeI YGGCCR 1 cut(s) 1060
Eam1104I CTCTTC 1 cut(s) 940
EarI CTCTTC 1 cut(s) 940
EciI GGCGGA 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 228, 994
Eco147I AGGCCT 1 cut(s) 1246
Eco24I GRGCYC 1 cut(s) 236
Eco47I GGWCC 2 cut(s) 172, 695
Eco57I CTGAAG 2 cut(s) 510, 743
EcoNI CCTNNNNNAGG 1 cut(s) 821
EcoRII CCWGG 2 cut(s) 424, 1040
EcoT14I CCWWGG 2 cut(s) 228, 994
EcoT38I GRGCYC 1 cut(s) 236
ErhI CCWWGG 2 cut(s) 228, 994
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 3 cut(s) 256, 959, 998
FalI AAGNNNNNCTT 2 cut(s) 903, 935
FaqI GGGAC 2 cut(s) 680, 795
FatI CATG 3 cut(s) 252, 955, 994
FauI CCCGC 1 cut(s) 90
FblI GTMKAC 3 cut(s) 246, 360, 787
FokI GGATG 3 cut(s) 800, 1300, 1315
FriOI GRGCYC 1 cut(s) 236
FspBI CTAG 3 cut(s) 761, 866, 1242
GlaI GCGC 2 cut(s) 219, 349
HaeIII GGCC 5 cut(s) 227, 1062, 1177, 1234, 1246
HapII CCGG 1 cut(s) 379
HgaI GACGC 2 cut(s) 265, 343
HhaI GCGC 2 cut(s) 220, 350
Hin1I GRCGYC 1 cut(s) 784
Hin1II CATG 3 cut(s) 256, 959, 998
Hin6I GCGC 2 cut(s) 218, 348
HinP1I GCGC 2 cut(s) 218, 348
HincII GTYRAC 1 cut(s) 1293
HindII GTYRAC 1 cut(s) 1293
HindIII AAGCTT 1 cut(s) 917
HinfI GANTC 4 cut(s) 438, 448, 775, 1259
HpaII CCGG 1 cut(s) 379
HphI GGTGA 1 cut(s) 1155
Hpy166II GTNNAC 6 cut(s) 247, 361, 788, 1152, 1285, 1293
Hpy188I TCNGA 7 cut(s) 99, 238, 723, 883, 1009, 1189, 1264
Hpy8I GTNNAC 6 cut(s) 247, 361, 788, 1152, 1285, 1293
Hpy99I CGWCG 2 cut(s) 359, 368
HpyAV CCTTC 2 cut(s) 431, 762
HpyCH4III ACNGT 7 cut(s) 30, 290, 412, 586, 626, 655, 850
HpyCH4IV ACGT 2 cut(s) 784, 1289
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 606, 1243
HpyF3I CTNAG 2 cut(s) 552, 1172
HpySE526I ACGT 2 cut(s) 784, 1289
Hsp92I GRCGYC 1 cut(s) 784
Hsp92II CATG 3 cut(s) 256, 959, 998
HspAI GCGC 2 cut(s) 218, 348
Kzo9I GATC 6 cut(s) 42, 87, 458, 505, 718, 1093
LmnI GCTCC 2 cut(s) 239, 677
Lsp1109I GCAGC 7 cut(s) 123, 196, 210, 356, 472, 494, 557
LweI GCATC 3 cut(s) 557, 739, 1337
MaeI CTAG 3 cut(s) 761, 866, 1242
MaeII ACGT 2 cut(s) 784, 1289
MaeIII GTNAC 1 cut(s) 626
MalI GATC 6 cut(s) 44, 89, 460, 507, 720, 1095
MboI GATC 6 cut(s) 42, 87, 458, 505, 718, 1093
MboII GAAGA 9 cut(s) 25, 86, 500, 503, 927, 930, 933, 936, 1127
MfeI CAATTG 1 cut(s) 1001
MflI RGATCY 3 cut(s) 505, 718, 1093
MhlI GDGCHC 2 cut(s) 236, 1154
MluCI AATT 6 cut(s) 36, 703, 739, 808, 1001, 1191
MluI ACGCGT 1 cut(s) 352
MlyI GAGTC 1 cut(s) 432
MmeI TCCRAC 2 cut(s) 162, 261
MroXI GAANNNNTTC 1 cut(s) 495
MseI TTAA 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 2 cut(s) 426, 1042
MunI CAATTG 1 cut(s) 1001
MvaI CCWGG 2 cut(s) 426, 1042
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 3 cut(s) 224, 606, 1243
NcoI CCATGG 1 cut(s) 994
NdeII GATC 6 cut(s) 42, 87, 458, 505, 718, 1093
NlaIII CATG 3 cut(s) 256, 959, 998
NlaIV GGNNCC 2 cut(s) 235, 1306
NmeAIII GCCGAG 1 cut(s) 638
NspV TTCGAA 1 cut(s) 903
PaqCI CACCTGC 1 cut(s) 1174
PceI AGGCCT 1 cut(s) 1246
PdmI GAANNNNTTC 1 cut(s) 495
PfeI GAWTC 3 cut(s) 448, 775, 1259
PflFI GACNNNGTC 1 cut(s) 242
PflMI CCANNNNNTGG 1 cut(s) 1069
PleI GAGTC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 432
PshBI ATTAAT 1 cut(s) 531
PsiI TTATAA 2 cut(s) 708, 923
Psp6I CCWGG 2 cut(s) 424, 1040
PspGI CCWGG 2 cut(s) 424, 1040
PspN4I GGNNCC 2 cut(s) 235, 1306
PspPI GGNCC 2 cut(s) 172, 695
PstI CTGCAG 1 cut(s) 484
PstNI CAGNNNCTG 2 cut(s) 488, 980
PsuI RGATCY 3 cut(s) 505, 718, 1093
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 3 cut(s) 119, 795, 1278
RsaNI GTAC 3 cut(s) 118, 794, 1277
SaqAI TTAA 1 cut(s) 531
Sau3AI GATC 6 cut(s) 42, 87, 458, 505, 718, 1093
Sau96I GGNCC 2 cut(s) 172, 695
ScaI AGTACT 1 cut(s) 795
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 2 cut(s) 426, 1042
SduI GDGCHC 2 cut(s) 236, 1154
SfaNI GCATC 3 cut(s) 557, 739, 1337
SfcI CTRYAG 3 cut(s) 411, 480, 1024
SfuI TTCGAA 1 cut(s) 903
SinI GGWCC 2 cut(s) 172, 695
Sse9I AATT 6 cut(s) 36, 703, 739, 808, 1001, 1191
SseBI AGGCCT 1 cut(s) 1246
SsiI CCGC 9 cut(s) 83, 282, 306, 309, 325, 567, 610, 1235, 1326
SspMI CTAG 3 cut(s) 761, 866, 1242
StuI AGGCCT 1 cut(s) 1246
StyD4I CCNGG 2 cut(s) 424, 1040
StyI CCWWGG 2 cut(s) 228, 994
TaaI ACNGT 7 cut(s) 30, 290, 412, 586, 626, 655, 850
TaiI ACGT 2 cut(s) 787, 1292
TaqI TCGA 2 cut(s) 903, 1080
TasI AATT 6 cut(s) 36, 703, 739, 808, 1001, 1191
TatI WGTACW 2 cut(s) 117, 793
TauI GCSGC 4 cut(s) 308, 311, 612, 1237
TfiI GAWTC 3 cut(s) 448, 775, 1259
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 4 cut(s) 33, 266, 397, 705
TseI GCWGC 7 cut(s) 111, 184, 198, 369, 482, 485, 545
TspDTI ATGAA 4 cut(s) 20, 924, 1098, 1122
TspRI CASTG 4 cut(s) 33, 266, 397, 705
Tth111I GACNNNGTC 1 cut(s) 242
Van91I CCANNNNNTGG 1 cut(s) 1069
VneI GTGCAC 1 cut(s) 1150
VpaK11BI GGWCC 2 cut(s) 172, 695
VspI ATTAAT 1 cut(s) 531
XagI CCTNNNNNAGG 1 cut(s) 821
XapI RAATTY 1 cut(s) 739
XmiI GTMKAC 3 cut(s) 246, 360, 787
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 3 cut(s) 761, 866, 1242
ZraI GACGTC 1 cut(s) 785
ZrmI AGTACT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.