RLG00000022971

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005518
Physical Location & Seq
Reverse (-)
16059352 .. 16064701
5350 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022971

Sequence Viewer

Length: 1872 bp
ATGATGAATAAGAAGCTATTGACAATAATTACAGTTAGCCACATATGGTGGTGGGCTAGTTTTGTGGGCGCAGACCCGCAGATCAATCTTCTGAACGAGGGTTGCAGCCAGTACAACGCAACCAGCTTGTCTGACTTCTATACCAATCTCAACGCCACTTTCTTGGACCTCAAAGCACAACTACTCAACGGCAGCAAACACTTTGCAACGGCACAACAGGCCAAGGGCTCCGATCCCGTCTACGCCATGACGCAGTGTAGGAACTACCTCTCCTCCACCGACTGCCTGTCCTGCTTTGCCGCCGCCGTGTCCCAAATCCGCAACTGCTCTGCCTCCAATGGCGCTCGCGTCGTCTACGACGGCTGCTTCCTCCGGTATGAGAGCAGTGGTTTTTTTGACCAGACTACGGCTACCCTTCCTGGTAATGTAGGACTCTGTGGGAATCAAACTGGATCACCTGCTACAACCTTCACTGCAGCTGCTGAAGAACTGCTGATGGATCTCCAAGTTGCTACGCCCAAGATTAATGGTTTCTTTGCAGCATCTAAGGAGGTAGTTAGCGGTAGTGGAAACACAACAGTCTATGGTGTGGCACAATGTGCCGAGACAACCACCGAAACTGGTTGCCAAGATTGTTTGAAGGTGGCATACACCAATGTTCAGAGTTGCCTTCCCGATACAGATGGTAGGGCAGTTGATGCCGGTTGTTTCTTGAGGTACTCTGCTACTTCTTTCTTCCCAGATAACCAGACTACTAATATCACGCCTTTCTTAGAAAAGGGAGGTAATTCAAGAAAGAAGGCCATCATCATTGGAGTAGTGGTTGGAGGAGACATATTGGGAGCAACCGAGTTGCAAGGTCCAGTGAATTATAAGTATAAAGATTTGAAGTCGGCAACAGCAAATTTTAGTGATGCAAATAAACTAGGAGAAGGAGGATTCGGGGACGTCTACAAGGCAACTCTAAACAATGGGAAAACAGTTGCAGTAAAGAAACTAGCTATACTCCAGTCAGACAGGGCAATGACAAGTTTCGAAAATGAAGTCAAGCTTATAAGTAATGTACATCACCGGAATCTAATTCGCCTTCTTGGATGTTGTAGCAAAGGACCAGAATTACTGCTGATATATGAATACATGGCAAACAACAGTCTGGACAAATTCTTATTTGGTCAGAAAAGTGGCTCTCTCAACTGGAAGCAACGGAATGATATAATCCTCGGTACAGCTAGGGGTCTAGCCTATCTACACGAGGAATTCCATGTATGTATCATACATAGAGATATAAAACCCAGCAACATTCTTTTGGATGATGGTTTTCAGCCAAGAATTGCAGATTTTGGGTTGGCAAGGCTTTTACCCGAGGATCAAACTCATCTTAGTACGCGATTTGCAGGAACATTAGGATACACAGCACCTGAATATGCAATCCATGGTCAATTGTCAGAAAAGGTGGATACCTATAGCTATGGAGTAGTAGTCCTTGAAATTGTAAGCGGCCAAAAGAGCAGTGAAATCAAATCTGATACTATGGGTGAATTTCTCCTCGAAAAGGCCTGGAAACTCTATGAGAACGGCCAGCACTTGGAGTTGGTCGATGAAACATTGGATCCAAATGATTATGAAGCAGAAGACGTGAAAAAGATTGTTGAGATTGCCCTAATGTGCACTCAATCATCACCTGCTCTAAGGCCAACAATGTCTGAAGTTGTTGTTTTACTCAAAAGCACAGGCTCGTGGGTAAATAGGCCGCTTACTAGGCCTGTGTTTGTTGATTCTGACCATAAAAGAGTACGGGTGGACACATCAACTTCTACTGGTTCCTCATCATCCAATGCTACCGCATCCATCTCTCAACTTTCAGGTCGCTAA

Protein Analysis

624

Amino Acids

67.82

Weight (kDa)

5.84

Isoelectric Point (pI)

28.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 35 - 128 3.8e-17 Salt stress response/antifungal
Stress-antifung PF01657 146 - 242 1.4e-13 Salt stress response/antifungal
Pkinase PF00069 304 - 571 3.6e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 307 - 572 1.5e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 873, 1055
AarI CACCTGC 2 cut(s) 466, 1690
AatII GACGTC 1 cut(s) 951
Acc36I ACCTGC 2 cut(s) 466, 1690
AccB7I CCANNNNNTGG 1 cut(s) 1585
AccI GTMKAC 3 cut(s) 240, 354, 951
AccII CGCG 2 cut(s) 348, 1387
AciI CCGC 8 cut(s) 77, 300, 303, 319, 561, 1497, 1751, 1842
AclWI GGATC 6 cut(s) 227, 460, 507, 1374, 1604, 1617
AcoI YGGCCR 2 cut(s) 1498, 1576
AcsI RAATTY 4 cut(s) 904, 1160, 1256, 1538
AcuI CTGAAG 2 cut(s) 504, 1725
AcyI GRCGYC 1 cut(s) 948
AdeI CACNNNGTG 1 cut(s) 599
AfaI GTAC 6 cut(s) 113, 719, 1065, 1225, 1384, 1794
AfiI CCNNNNNNNGG 3 cut(s) 406, 1552, 1585
AgsI TTSAA 4 cut(s) 640, 792, 889, 1487
AhdI GACNNNNNGTC 1 cut(s) 286
AjiI CACGTC 1 cut(s) 1636
AjnI CCWGG 2 cut(s) 418, 1556
AloI GAACNNNNNNTCC 2 cut(s) 254, 286
AluBI AGCT 7 cut(s) 16, 126, 479, 1001, 1051, 1229, 1467
AluI AGCT 7 cut(s) 16, 126, 479, 1001, 1051, 1229, 1467
Alw21I GWGCWC 1 cut(s) 1670
Alw26I GTCTC 2 cut(s) 599, 825
Alw44I GTGCAC 1 cut(s) 1666
AlwI GGATC 6 cut(s) 227, 460, 507, 1374, 1604, 1617
AlwNI CAGNNNCTG 2 cut(s) 482, 1418
Ama87I CYCGRG 1 cut(s) 1361
AoxI GGCC 8 cut(s) 219, 801, 1498, 1554, 1576, 1691, 1748, 1760
ApaLI GTGCAC 1 cut(s) 1666
ApeKI GCWGC 6 cut(s) 105, 192, 363, 476, 479, 539
ApoI RAATTY 4 cut(s) 904, 1160, 1256, 1538
ArsI GACNNNNNNTTYG 2 cut(s) 1029, 1061
AseI ATTAAT 1 cut(s) 525
AspLEI GCGC 2 cut(s) 71, 344
AspS9I GGNCC 3 cut(s) 166, 860, 1109
AsuHPI GGTGA 4 cut(s) 447, 1061, 1547, 1671
AsuII TTCGAA 1 cut(s) 1035
AvaI CYCGRG 1 cut(s) 1361
AvaII GGWCC 3 cut(s) 166, 860, 1109
BaeGI GKGCMC 1 cut(s) 1670
BamHI GGATCC 1 cut(s) 1609
BanII GRGCYC 1 cut(s) 230
BarI GAAGNNNNNNTAC 2 cut(s) 632, 664
BauI CACGAG 2 cut(s) 1250, 1735
BbsI GAAGAC 1 cut(s) 1638
Bbv12I GWGCWC 1 cut(s) 1670
BbvI GCAGC 6 cut(s) 117, 204, 350, 466, 488, 551
BccI CCATC 5 cut(s) 490, 677, 812, 1307, 1856
BceAI ACGGC 6 cut(s) 205, 225, 290, 376, 423, 1591
BciT130I CCWGG 2 cut(s) 420, 1558
BciVI GTATCC 2 cut(s) 1400, 1450
BcoDI GTCTC 2 cut(s) 599, 825
BfaI CTAG 6 cut(s) 57, 926, 998, 1230, 1238, 1758
BfmI CTRYAG 2 cut(s) 474, 1462
BfoI RGCGCY 1 cut(s) 345
BfuAI ACCTGC 2 cut(s) 466, 1690
BfuI GTATCC 2 cut(s) 1400, 1450
Bme1390I CCNGG 2 cut(s) 420, 1558
Bme18I GGWCC 3 cut(s) 166, 860, 1109
BmeRI GACNNNNNGTC 1 cut(s) 286
BmeT110I CYCGRG 1 cut(s) 1361
BmgBI CACGTC 1 cut(s) 1636
BmgT120I GGNCC 3 cut(s) 166, 860, 1109
BmiI GGNNCC 3 cut(s) 229, 1611, 1822
BmrFI CCNGG 2 cut(s) 420, 1558
BmsI GCATC 4 cut(s) 551, 688, 904, 1853
BpiI GAAGAC 1 cut(s) 1638
BpmI CTGGAG 1 cut(s) 992
Bpu14I TTCGAA 1 cut(s) 1035
BpuEI CTTGAG 1 cut(s) 733
BsaHI GRCGYC 1 cut(s) 948
BsaJI CCNNGG 4 cut(s) 222, 1219, 1362, 1432
BsaWI WCCGGW 2 cut(s) 372, 1071
BsaXI ACNNNNNCTCC 8 cut(s) 254, 257, 284, 287, 807, 837, 1464, 1494
Bsc4I CCNNNNNNNGG 3 cut(s) 406, 1552, 1585
Bse118I RCCGGY 1 cut(s) 701
Bse1I ACTGG 7 cut(s) 109, 454, 625, 863, 1009, 1199, 1822
Bse3DI GCAATG 1 cut(s) 1029
BseBI CCWGG 2 cut(s) 420, 1558
BseDI CCNNGG 4 cut(s) 222, 1219, 1362, 1432
BseGI GGATG 4 cut(s) 1100, 1315, 1829, 1844
BseLI CCNNNNNNNGG 3 cut(s) 406, 1552, 1585
BseMI GCAATG 1 cut(s) 1029
BseNI ACTGG 7 cut(s) 109, 454, 625, 863, 1009, 1199, 1822
BseRI GAGGAG 3 cut(s) 262, 843, 1535
BseSI GKGCMC 1 cut(s) 1670
BseXI GCAGC 6 cut(s) 117, 204, 350, 466, 488, 551
BseYI CCCAGC 1 cut(s) 1292
Bsh1236I CGCG 2 cut(s) 348, 1387
BshFI GGCC 8 cut(s) 221, 803, 1500, 1556, 1578, 1693, 1750, 1762
BsiHKAI GWGCWC 1 cut(s) 1670
BsiHKCI CYCGRG 1 cut(s) 1361
BsiSI CCGG 3 cut(s) 373, 702, 1072
BslFI GGGAC 2 cut(s) 295, 959
BslI CCNNNNNNNGG 3 cut(s) 406, 1552, 1585
BsmAI GTCTC 2 cut(s) 599, 825
BsmFI GGGAC 2 cut(s) 295, 959
BsnI GGCC 8 cut(s) 221, 803, 1500, 1556, 1578, 1693, 1750, 1762
BsoBI CYCGRG 1 cut(s) 1361
Bsp119I TTCGAA 1 cut(s) 1035
Bsp1286I GDGCHC 2 cut(s) 230, 1670
Bsp1407I TGTACA 1 cut(s) 1063
Bsp143I GATC 6 cut(s) 81, 232, 452, 499, 1366, 1609
Bsp19I CCATGG 1 cut(s) 1432
BspACI CCGC 8 cut(s) 77, 300, 303, 319, 561, 1497, 1751, 1842
BspANI GGCC 8 cut(s) 221, 803, 1500, 1556, 1578, 1693, 1750, 1762
BspFNI CGCG 2 cut(s) 348, 1387
BspLI GGNNCC 3 cut(s) 229, 1611, 1822
BspMAI CTGCAG 1 cut(s) 478
BspMI ACCTGC 2 cut(s) 466, 1690
BspPI GGATC 6 cut(s) 227, 460, 507, 1374, 1604, 1617
BspT104I TTCGAA 1 cut(s) 1035
BsrDI GCAATG 1 cut(s) 1029
BsrFI RCCGGY 1 cut(s) 701
BsrGI TGTACA 1 cut(s) 1063
BsrI ACTGG 7 cut(s) 109, 454, 625, 863, 1009, 1199, 1822
BssAI RCCGGY 1 cut(s) 701
BssECI CCNNGG 4 cut(s) 222, 1219, 1362, 1432
BssMI GATC 6 cut(s) 81, 232, 452, 499, 1366, 1609
BssNI GRCGYC 1 cut(s) 948
BssSI CACGAG 2 cut(s) 1250, 1735
BssT1I CCWWGG 2 cut(s) 222, 1432
Bst2BI CACGAG 2 cut(s) 1250, 1735
Bst2UI CCWGG 2 cut(s) 420, 1558
Bst4CI ACNGT 4 cut(s) 34, 580, 982, 1151
BstACI GRCGYC 1 cut(s) 948
BstAPI GCANNNNNTGC 2 cut(s) 599, 698
BstAUI TGTACA 1 cut(s) 1063
BstBI TTCGAA 1 cut(s) 1035
BstC8I GCNNGC 2 cut(s) 346, 1580
BstDEI CTNAG 4 cut(s) 546, 772, 1379, 1688
BstDSI CCRYGG 1 cut(s) 1432
BstENI CCTNNNNNAGG 1 cut(s) 1550
BstF5I GGATG 4 cut(s) 1100, 1315, 1829, 1844
BstFNI CGCG 2 cut(s) 348, 1387
BstH2I RGCGCY 1 cut(s) 345
BstHHI GCGC 2 cut(s) 71, 344
BstKTI GATC 6 cut(s) 84, 235, 455, 502, 1369, 1612
BstMAI GTCTC 2 cut(s) 599, 825
BstMBI GATC 6 cut(s) 81, 232, 452, 499, 1366, 1609
BstMWI GCNNNNNNNGC 6 cut(s) 218, 291, 599, 698, 1506, 1759
BstNI CCWGG 2 cut(s) 420, 1558
BstSCI CCNGG 2 cut(s) 418, 1556
BstSFI CTRYAG 2 cut(s) 474, 1462
BstSLI GKGCMC 1 cut(s) 1670
BstUI CGCG 2 cut(s) 348, 1387
BstV1I GCAGC 6 cut(s) 117, 204, 350, 466, 488, 551
BstV2I GAAGAC 1 cut(s) 1638
BstX2I RGATCY 2 cut(s) 499, 1609
BstXI CCANNNNNNTGG 1 cut(s) 163
BstYI RGATCY 2 cut(s) 499, 1609
BsuI GTATCC 2 cut(s) 1400, 1450
BsuRI GGCC 8 cut(s) 221, 803, 1500, 1556, 1578, 1693, 1750, 1762
BtgI CCRYGG 1 cut(s) 1432
BtrI CACGTC 1 cut(s) 1636
BtsCI GGATG 4 cut(s) 1100, 1315, 1829, 1844
BtsI GCAGTG 4 cut(s) 260, 391, 471, 1516
BtsIMutI CAGTG 5 cut(s) 260, 391, 471, 870, 1516
BveI ACCTGC 2 cut(s) 466, 1690
Cac8I GCNNGC 2 cut(s) 346, 1580
CaiI CAGNNNCTG 2 cut(s) 482, 1418
CfoI GCGC 2 cut(s) 71, 344
Cfr10I RCCGGY 1 cut(s) 701
Cfr13I GGNCC 3 cut(s) 166, 860, 1109
CseI GACGC 2 cut(s) 259, 337
Csp6I GTAC 6 cut(s) 112, 718, 1064, 1224, 1383, 1793
CviAII CATG 4 cut(s) 247, 1138, 1262, 1433
CviQI GTAC 6 cut(s) 112, 718, 1064, 1224, 1383, 1793
DdeI CTNAG 4 cut(s) 546, 772, 1379, 1688
DpnI GATC 6 cut(s) 83, 234, 454, 501, 1368, 1611
DpnII GATC 6 cut(s) 81, 232, 452, 499, 1366, 1609
DraIII CACNNNGTG 1 cut(s) 599
DriI GACNNNNNGTC 1 cut(s) 286
EaeI YGGCCR 2 cut(s) 1498, 1576
Eam1105I GACNNNNNGTC 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 222, 1432
Eco147I AGGCCT 2 cut(s) 1556, 1762
Eco24I GRGCYC 1 cut(s) 230
Eco47I GGWCC 3 cut(s) 166, 860, 1109
Eco57I CTGAAG 2 cut(s) 504, 1725
Eco88I CYCGRG 1 cut(s) 1361
EcoNI CCTNNNNNAGG 1 cut(s) 1550
EcoRI GAATTC 1 cut(s) 1256
EcoRII CCWGG 2 cut(s) 418, 1556
EcoT14I CCWWGG 2 cut(s) 222, 1432
EcoT38I GRGCYC 1 cut(s) 230
ErhI CCWWGG 2 cut(s) 222, 1432
FaeI CATG 4 cut(s) 250, 1141, 1265, 1436
FalI AAGNNNNNCTT 2 cut(s) 1035, 1067
FaqI GGGAC 2 cut(s) 295, 959
FatI CATG 4 cut(s) 246, 1137, 1261, 1432
FauI CCCGC 1 cut(s) 84
FauNDI CATATG 1 cut(s) 44
FblI GTMKAC 3 cut(s) 240, 354, 951
FokI GGATG 4 cut(s) 1107, 1322, 1816, 1831
FriOI GRGCYC 1 cut(s) 230
FspBI CTAG 6 cut(s) 57, 926, 998, 1230, 1238, 1758
GlaI GCGC 2 cut(s) 70, 343
GsaI CCCAGC 1 cut(s) 1296
GsuI CTGGAG 1 cut(s) 992
HaeII RGCGCY 1 cut(s) 345
HaeIII GGCC 8 cut(s) 221, 803, 1500, 1556, 1578, 1693, 1750, 1762
HapII CCGG 3 cut(s) 373, 702, 1072
HgaI GACGC 2 cut(s) 259, 337
HhaI GCGC 2 cut(s) 71, 344
Hin1I GRCGYC 1 cut(s) 948
Hin1II CATG 4 cut(s) 250, 1141, 1265, 1436
Hin6I GCGC 2 cut(s) 69, 342
HinP1I GCGC 2 cut(s) 69, 342
HindIII AAGCTT 1 cut(s) 1049
HinfI GANTC 5 cut(s) 432, 442, 939, 1075, 1775
HpaII CCGG 3 cut(s) 373, 702, 1072
HphI GGTGA 4 cut(s) 447, 1061, 1547, 1671
Hpy166II GTNNAC 5 cut(s) 241, 355, 952, 1668, 1801
Hpy188III TCNNGA 4 cut(s) 674, 712, 792, 1154
Hpy8I GTNNAC 5 cut(s) 241, 355, 952, 1668, 1801
Hpy99I CGWCG 2 cut(s) 353, 362
HpyAV CCTTC 7 cut(s) 425, 478, 634, 680, 793, 926, 1097
HpyCH4III ACNGT 4 cut(s) 34, 580, 982, 1151
HpyCH4IV ACGT 2 cut(s) 948, 1635
HpyF10VI GCNNNNNNNGC 6 cut(s) 218, 291, 599, 698, 1506, 1759
HpyF3I CTNAG 4 cut(s) 546, 772, 1379, 1688
HpySE526I ACGT 2 cut(s) 948, 1635
Hsp92I GRCGYC 1 cut(s) 948
Hsp92II CATG 4 cut(s) 250, 1141, 1265, 1436
HspAI GCGC 2 cut(s) 69, 342
Kzo9I GATC 6 cut(s) 81, 232, 452, 499, 1366, 1609
LmnI GCTCC 2 cut(s) 233, 842
Lsp1109I GCAGC 6 cut(s) 117, 204, 350, 466, 488, 551
LweI GCATC 4 cut(s) 551, 688, 904, 1853
MaeI CTAG 6 cut(s) 57, 926, 998, 1230, 1238, 1758
MaeII ACGT 2 cut(s) 948, 1635
MalI GATC 6 cut(s) 83, 234, 454, 501, 1368, 1611
MboI GATC 6 cut(s) 81, 232, 452, 499, 1366, 1609
MboII GAAGA 4 cut(s) 80, 497, 727, 1643
MfeI CAATTG 1 cut(s) 1439
MflI RGATCY 2 cut(s) 499, 1609
MhlI GDGCHC 2 cut(s) 230, 1670
MlyI GAGTC 1 cut(s) 426
MmeI TCCRAC 1 cut(s) 805
MseI TTAA 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 3 cut(s) 373, 702, 1072
MspR9I CCNGG 2 cut(s) 420, 1558
MunI CAATTG 1 cut(s) 1439
MvaI CCWGG 2 cut(s) 420, 1558
MvnI CGCG 2 cut(s) 348, 1387
MwoI GCNNNNNNNGC 6 cut(s) 218, 291, 599, 698, 1506, 1759
NcoI CCATGG 1 cut(s) 1432
NdeI CATATG 1 cut(s) 44
NdeII GATC 6 cut(s) 81, 232, 452, 499, 1366, 1609
NlaIII CATG 4 cut(s) 250, 1141, 1265, 1436
NlaIV GGNNCC 3 cut(s) 229, 1611, 1822
NmeAIII GCCGAG 1 cut(s) 628
NspV TTCGAA 1 cut(s) 1035
PaqCI CACCTGC 2 cut(s) 466, 1690
PceI AGGCCT 2 cut(s) 1556, 1762
PfeI GAWTC 4 cut(s) 442, 939, 1075, 1775
PflMI CCANNNNNTGG 1 cut(s) 1585
PleI GAGTC 1 cut(s) 426
PpsI GAGTC 1 cut(s) 426
PshBI ATTAAT 1 cut(s) 525
PsiI TTATAA 2 cut(s) 873, 1055
Psp6I CCWGG 2 cut(s) 418, 1556
PspFI CCCAGC 1 cut(s) 1292
PspGI CCWGG 2 cut(s) 418, 1556
PspN4I GGNNCC 3 cut(s) 229, 1611, 1822
PspPI GGNCC 3 cut(s) 166, 860, 1109
PstI CTGCAG 1 cut(s) 478
PstNI CAGNNNCTG 2 cut(s) 482, 1418
PsuI RGATCY 2 cut(s) 499, 1609
PvuII CAGCTG 1 cut(s) 479
RsaI GTAC 6 cut(s) 113, 719, 1065, 1225, 1384, 1794
RsaNI GTAC 6 cut(s) 112, 718, 1064, 1224, 1383, 1793
SaqAI TTAA 1 cut(s) 525
Sau3AI GATC 6 cut(s) 81, 232, 452, 499, 1366, 1609
Sau96I GGNCC 3 cut(s) 166, 860, 1109
SchI GAGTC 1 cut(s) 426
ScrFI CCNGG 2 cut(s) 420, 1558
SduI GDGCHC 2 cut(s) 230, 1670
SfaNI GCATC 4 cut(s) 551, 688, 904, 1853
SfcI CTRYAG 2 cut(s) 474, 1462
SfuI TTCGAA 1 cut(s) 1035
SinI GGWCC 3 cut(s) 166, 860, 1109
SmlI CTYRAG 1 cut(s) 712
SmoI CTYRAG 1 cut(s) 712
SseBI AGGCCT 2 cut(s) 1556, 1762
SsiI CCGC 8 cut(s) 77, 300, 303, 319, 561, 1497, 1751, 1842
SspMI CTAG 6 cut(s) 57, 926, 998, 1230, 1238, 1758
StuI AGGCCT 2 cut(s) 1556, 1762
StyD4I CCNGG 2 cut(s) 418, 1556
StyI CCWWGG 2 cut(s) 222, 1432
TaaI ACNGT 4 cut(s) 34, 580, 982, 1151
TaiI ACGT 2 cut(s) 951, 1638
TaqI TCGA 3 cut(s) 1035, 1548, 1596
TatI WGTACW 2 cut(s) 111, 1063
TauI GCSGC 4 cut(s) 302, 305, 1500, 1753
TfiI GAWTC 4 cut(s) 442, 939, 1075, 1775
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TscAI CASTG 5 cut(s) 260, 391, 478, 870, 1516
TseI GCWGC 6 cut(s) 105, 192, 363, 476, 479, 539
TspDTI ATGAA 5 cut(s) 20, 1056, 1146, 1614, 1638
TspGWI ACGGA 1 cut(s) 1219
TspRI CASTG 5 cut(s) 260, 391, 478, 870, 1516
Van91I CCANNNNNTGG 1 cut(s) 1585
VneI GTGCAC 1 cut(s) 1666
VpaK11BI GGWCC 3 cut(s) 166, 860, 1109
VspI ATTAAT 1 cut(s) 525
XagI CCTNNNNNAGG 1 cut(s) 1550
XapI RAATTY 4 cut(s) 904, 1160, 1256, 1538
XmiI GTMKAC 3 cut(s) 240, 354, 951
XspI CTAG 6 cut(s) 57, 926, 998, 1230, 1238, 1758
ZraI GACGTC 1 cut(s) 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.