pycom10g05050

39S ribosomal protein L46

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002375
Physical Location & Seq
Forward (+)
5321426 .. 5323736
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g05050.5

Sequence Viewer

Length: 684 bp
ATGCAGCGGACGATCACAATATTGCGTGGCCCTCTGGGCAGAGGCCGAGGGTTTTCCTCCACCTCATTGTCTTCCTCCGACAAAATCGTTGCGTCCGTGCTCTTCGAGAGGCTGCCGGTTGTTATTCCCAAAATGGACCCCGTCGTTTATGCGTATCAGGAATTCGCGTTCCGGTGGCGACAGCAATATCGGCGCATATATCCCGATGAGCTTTTAGACAAGTCTAATGCTAGGGGAAAAGGTGACTACCAGATAGATTATGAACCTGCTCCGCGGATCACTGAAGCTGACAAAAAGAATGATAAAAAGTCATTGCAGAGAGCCCTTGACAGAAGACTATATCTTCTTCTCTACGGAACCACTGATCGATCTGCTGGAAAATCAGTCTGGCATTTTCCAGAAAAAGTTTACCAGTCTGAAGAGACACTGCGGAAGTGCGCAGAATCTGCATTAAAATCAGTTTTGGGAGATCTGTCACACACGTACTTTGTTGGAAATGCTCCCATGGGCCATATGGAACAGACAGCAGAGAATATTAAGCAATTCTTTTTCAAGTCTCAAGTGATTGCAACGAACAAGTTGAACATTGGGAAGTGCGAGGATTTTGTCTGGGTGACCAAGGATGAATTGATGGAGTACTTTCCTGAGCAAGCTGATTTCCTTAGCAAGATGATCATTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

228

Amino Acids

26.23

Weight (kDa)

9.05

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 439
Acc36I ACCTGC 1 cut(s) 274
AccII CGCG 2 cut(s) 167, 274
AciI CCGC 4 cut(s) 7, 272, 274, 430
AclWI GGATC 1 cut(s) 284
AcsI RAATTY 1 cut(s) 161
AcuI CTGAAG 2 cut(s) 303, 438
AfaI GTAC 2 cut(s) 485, 638
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 2 cut(s) 553, 583
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 4 cut(s) 211, 287, 653, 681
AluI AGCT 4 cut(s) 211, 287, 653, 681
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 416, 561
AlwI GGATC 1 cut(s) 284
AlwNI CAGNNNCTG 1 cut(s) 446
AoxI GGCC 3 cut(s) 28, 43, 508
ApeKI GCWGC 2 cut(s) 4, 112
ApoI RAATTY 1 cut(s) 161
AspLEI GCGC 2 cut(s) 195, 440
AspS9I GGNCC 3 cut(s) 29, 136, 508
AsuHPI GGTGA 2 cut(s) 254, 625
AvaII GGWCC 1 cut(s) 136
BanII GRGCYC 1 cut(s) 325
BbsI GAAGAC 2 cut(s) 63, 340
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 2 cut(s) 16, 99
BccI CCATC 1 cut(s) 625
BclI TGATCA 1 cut(s) 672
BcoDI GTCTC 2 cut(s) 416, 561
BfaI CTAG 1 cut(s) 231
BfuAI ACCTGC 1 cut(s) 274
BglI GCCNNNNNGGC 1 cut(s) 36
BglII AGATCT 1 cut(s) 469
BisI GCNGC 2 cut(s) 5, 113
BlsI GCNGC 2 cut(s) 6, 114
BmcAI AGTACT 1 cut(s) 638
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 3 cut(s) 29, 136, 508
BmiI GGNNCC 2 cut(s) 138, 358
BpiI GAAGAC 2 cut(s) 63, 340
Bpu10I CCTNAGC 2 cut(s) 645, 662
BpuEI CTTGAG 1 cut(s) 543
Bsa29I ATCGAT 1 cut(s) 367
BsaAI YACGTR 1 cut(s) 483
BsaJI CCNNGG 4 cut(s) 46, 272, 504, 618
BsaWI WCCGGW 1 cut(s) 171
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 1 cut(s) 412
Bse3DI GCAATG 1 cut(s) 311
BseCI ATCGAT 1 cut(s) 367
BseDI CCNNGG 4 cut(s) 46, 272, 504, 618
BseGI GGATG 1 cut(s) 628
BseMI GCAATG 1 cut(s) 311
BseMII CTCAG 1 cut(s) 636
BseNI ACTGG 1 cut(s) 412
BseXI GCAGC 2 cut(s) 16, 99
Bsh1236I CGCG 2 cut(s) 167, 274
BshFI GGCC 3 cut(s) 30, 45, 510
BshVI ATCGAT 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 2 cut(s) 116, 172
BsmAI GTCTC 2 cut(s) 416, 561
BsnI GGCC 3 cut(s) 30, 45, 510
Bsp1286I GDGCHC 2 cut(s) 102, 325
Bsp143I GATC 6 cut(s) 12, 276, 364, 368, 469, 672
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 4 cut(s) 7, 272, 274, 430
BspANI GGCC 3 cut(s) 30, 45, 510
BspCNI CTCAG 1 cut(s) 637
BspDI ATCGAT 1 cut(s) 367
BspFNI CGCG 2 cut(s) 167, 274
BspLI GGNNCC 2 cut(s) 138, 358
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 1 cut(s) 284
BspQI GCTCTTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 311
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 1 cut(s) 412
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 4 cut(s) 46, 272, 504, 618
BssMI GATC 6 cut(s) 12, 276, 364, 368, 469, 672
BssT1I CCWWGG 2 cut(s) 504, 618
Bst6I CTCTTC 2 cut(s) 107, 414
BstAPI GCANNNNNTGC 1 cut(s) 446
BstBAI YACGTR 1 cut(s) 483
BstC8I GCNNGC 1 cut(s) 651
BstDEI CTNAG 2 cut(s) 645, 662
BstDSI CCRYGG 2 cut(s) 272, 504
BstEII GGTNACC 1 cut(s) 613
BstF5I GGATG 1 cut(s) 628
BstFNI CGCG 2 cut(s) 167, 274
BstHHI GCGC 2 cut(s) 195, 440
BstKTI GATC 6 cut(s) 15, 279, 367, 371, 472, 675
BstMAI GTCTC 2 cut(s) 416, 561
BstMBI GATC 6 cut(s) 12, 276, 364, 368, 469, 672
BstMWI GCNNNNNNNGC 3 cut(s) 36, 190, 446
BstPI GGTNACC 1 cut(s) 613
BstUI CGCG 2 cut(s) 167, 274
BstV1I GCAGC 2 cut(s) 16, 99
BstV2I GAAGAC 2 cut(s) 63, 340
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
Bsu15I ATCGAT 1 cut(s) 367
BsuRI GGCC 3 cut(s) 30, 45, 510
BsuTUI ATCGAT 1 cut(s) 367
BtgI CCRYGG 2 cut(s) 272, 504
BtsCI GGATG 1 cut(s) 628
BtsI GCAGTG 1 cut(s) 425
BtsIMutI CAGTG 3 cut(s) 279, 360, 425
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 651
CaiI CAGNNNCTG 1 cut(s) 446
CfoI GCGC 2 cut(s) 195, 440
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 3 cut(s) 29, 136, 508
Cfr42I CCGCGG 1 cut(s) 275
ClaI ATCGAT 1 cut(s) 367
CseI GACGC 1 cut(s) 81
Csp6I GTAC 2 cut(s) 484, 637
CviAII CATG 1 cut(s) 505
CviJI RGCY 9 cut(s) 30, 45, 112, 211, 287, 323, 510, 653, 681
CviKI_1 RGCY 9 cut(s) 30, 45, 112, 211, 287, 323, 510, 653, 681
CviQI GTAC 2 cut(s) 484, 637
DdeI CTNAG 2 cut(s) 645, 662
DpnI GATC 6 cut(s) 14, 278, 366, 370, 471, 674
DpnII GATC 6 cut(s) 12, 276, 364, 368, 469, 672
Eam1104I CTCTTC 2 cut(s) 107, 414
EarI CTCTTC 2 cut(s) 107, 414
Eco130I CCWWGG 2 cut(s) 504, 618
Eco24I GRGCYC 1 cut(s) 325
Eco47I GGWCC 1 cut(s) 136
Eco57I CTGAAG 2 cut(s) 303, 438
Eco91I GGTNACC 1 cut(s) 613
EcoO65I GGTNACC 1 cut(s) 613
EcoRI GAATTC 1 cut(s) 161
EcoT14I CCWWGG 2 cut(s) 504, 618
EcoT38I GRGCYC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 504, 618
FaeI CATG 1 cut(s) 508
FaiI YATR 8 cut(s) 150, 197, 199, 261, 340, 506, 513, 515
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FatI CATG 1 cut(s) 504
FauNDI CATATG 1 cut(s) 513
FbaI TGATCA 1 cut(s) 672
Fnu4HI GCNGC 2 cut(s) 5, 113
FokI GGATG 1 cut(s) 635
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 2 cut(s) 5, 113
FspBI CTAG 1 cut(s) 231
FspI TGCGCA 1 cut(s) 439
GlaI GCGC 2 cut(s) 194, 439
GluI GCNGC 2 cut(s) 5, 113
HaeIII GGCC 3 cut(s) 30, 45, 510
HapII CCGG 2 cut(s) 116, 172
HgaI GACGC 1 cut(s) 81
HhaI GCGC 2 cut(s) 195, 440
Hin1II CATG 1 cut(s) 508
Hin6I GCGC 2 cut(s) 193, 438
HinP1I GCGC 2 cut(s) 193, 438
HinfI GANTC 1 cut(s) 443
HpaII CCGG 2 cut(s) 116, 172
HphI GGTGA 2 cut(s) 254, 625
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 2 cut(s) 79, 418
Hpy188III TCNNGA 5 cut(s) 106, 158, 203, 398, 644
Hpy8I GTNNAC 1 cut(s) 409
Hpy99I CGWCG 1 cut(s) 146
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 4 cut(s) 4, 316, 449, 569
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 190, 446
HpyF3I CTNAG 2 cut(s) 645, 662
HpySE526I ACGT 1 cut(s) 482
Hsp92II CATG 1 cut(s) 508
HspAI GCGC 2 cut(s) 193, 438
Ksp22I TGATCA 1 cut(s) 672
KspI CCGCGG 1 cut(s) 275
Kzo9I GATC 6 cut(s) 12, 276, 364, 368, 469, 672
LguI GCTCTTC 1 cut(s) 107
LmnI GCTCC 2 cut(s) 274, 505
Lsp1109I GCAGC 2 cut(s) 16, 99
MaeI CTAG 1 cut(s) 231
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 3 cut(s) 242, 474, 613
MalI GATC 6 cut(s) 14, 278, 366, 370, 471, 674
MboI GATC 6 cut(s) 12, 276, 364, 368, 469, 672
MboII GAAGA 6 cut(s) 63, 94, 335, 338, 345, 431
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 2 cut(s) 102, 325
MluCI AATT 3 cut(s) 161, 542, 626
MmeI TCCRAC 2 cut(s) 102, 472
MnlI CCTC 8 cut(s) 35, 41, 42, 67, 73, 85, 102, 592
MseI TTAA 2 cut(s) 452, 537
MspA1I CMGCKG 2 cut(s) 7, 274
MspI CCGG 2 cut(s) 116, 172
MvnI CGCG 2 cut(s) 167, 274
MwoI GCNNNNNNNGC 3 cut(s) 36, 190, 446
NcoI CCATGG 1 cut(s) 504
NdeI CATATG 1 cut(s) 513
NdeII GATC 6 cut(s) 12, 276, 364, 368, 469, 672
NlaIII CATG 1 cut(s) 508
NlaIV GGNNCC 2 cut(s) 138, 358
NmeAIII GCCGAG 1 cut(s) 71
NmuCI GTSAC 3 cut(s) 242, 474, 613
NsbI TGCGCA 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 1 cut(s) 443
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 6, 114
Ppu21I YACGTR 1 cut(s) 483
PspEI GGTNACC 1 cut(s) 613
PspN4I GGNNCC 2 cut(s) 138, 358
PspPI GGNCC 3 cut(s) 29, 136, 508
PstNI CAGNNNCTG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 469
PsyI GACNNNGTC 1 cut(s) 140
RsaI GTAC 2 cut(s) 485, 638
RsaNI GTAC 2 cut(s) 484, 637
SacII CCGCGG 1 cut(s) 275
SapI GCTCTTC 1 cut(s) 107
SaqAI TTAA 2 cut(s) 452, 537
SatI GCNGC 2 cut(s) 5, 113
Sau3AI GATC 6 cut(s) 12, 276, 364, 368, 469, 672
Sau96I GGNCC 3 cut(s) 29, 136, 508
ScaI AGTACT 1 cut(s) 638
SduI GDGCHC 2 cut(s) 102, 325
SetI ASST 8 cut(s) 65, 213, 244, 268, 289, 485, 655, 683
Sfr303I CCGCGG 1 cut(s) 275
SgrBI CCGCGG 1 cut(s) 275
SinI GGWCC 1 cut(s) 136
SmlI CTYRAG 1 cut(s) 558
SmoI CTYRAG 1 cut(s) 558
Sse9I AATT 3 cut(s) 161, 542, 626
SsiI CCGC 4 cut(s) 7, 272, 274, 430
SspI AATATT 2 cut(s) 21, 535
SspMI CTAG 1 cut(s) 231
StyI CCWWGG 2 cut(s) 504, 618
TaiI ACGT 1 cut(s) 485
TaqI TCGA 2 cut(s) 105, 367
TasI AATT 3 cut(s) 161, 542, 626
TatI WGTACW 1 cut(s) 636
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 2 cut(s) 452, 537
Tru9I TTAA 2 cut(s) 452, 537
TscAI CASTG 3 cut(s) 286, 367, 432
TseFI GTSAC 3 cut(s) 242, 474, 613
TseI GCWGC 2 cut(s) 4, 112
Tsp45I GTSAC 3 cut(s) 242, 474, 613
TspDTI ATGAA 2 cut(s) 276, 639
TspGWI ACGGA 2 cut(s) 85, 369
TspRI CASTG 3 cut(s) 286, 367, 432
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 136
XapI RAATTY 1 cut(s) 161
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
XspI CTAG 1 cut(s) 231
ZrmI AGTACT 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.