Rorug02G0267200

39S ribosomal protein L46

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002375
Physical Location & Seq
Forward (+)
28178903 .. 28187025
8123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0267200.1

Sequence Viewer

Length: 1329 bp
ATGGAAGGAGGAAGTGACTTAGATGCCCAAATAGAGAAATTGCTGAATGTGGAGAAACAAATGAGGCTCTCTGGTGATGTGGCGGGAACCAAGAAGGCTGTCACTGACATTCTTGAGCTTTGCTTTAAAGCAGGTGCTTGGAAGACCCTCAATGACCAGATTCTGGTCTTGTCCAAACGCCGTGGTCAGCTCAAGCAGGCTGTAACTGCATTGGTCCAGCAAGCAATGCAATATATTGATCAGACACCTGATCTTGAAACTCGTATAGAGCTGATTAAAACACTAAATAATGTTTCTGCCGGGAAGATATATGTTGAAATTGAGAGAGCACGGTTAATCAAAAGACTTGCAAAGATCAAGGAAGAGCAAGGGCTTATTGCTGAAGCTGCTGATTTGATGCAAGAAACTGCGGTGGAAACATTTGGTGCTATGGCAAAAACTGAGAAGATTGCATTCATTCTTGAACAAGTTCGTTTGTGCGAAGACCGTCAGGATTATGTTCGTGCTCAAATACTGTCGAGGAAGATTAGTCCAAGGGTGTTTGATGTTGATACTTCAAAAGAAAAGAAAAAACCCAAGGAAGGTGATAATGTTGTTGAAGAAGCTCCTGCTGATGTACCATCGCTCTTGGAGTTGAAGCGGATCTACTATGAATTAATGATACGGTATTATTCTCATAACAATGATTACCTTGAGATTTGCCGTTGCTATAAGGCGATATACGAGATCCCTTCTGTTAAAGAGGATCCAGCTCAGTGGATACCGATATTGAGGAAAATCTGTTGGTACTTGGTTCTGTCACCATACGATCCAATGCAGTCAAGCCTTCTTAATTCCACCCTGGAGGATAAGAATCTTTCAGAGGTTCCAAATTTTAGGTTACTTTTGAAACAAGTGGTAACTATGGAGGTCATACAATGGACCACACTCTGGAATAAGTTTAAGGATGAGTTTGAGAATGAGAAGAACATGCTTGGAGGCTCTTTGGGTGACAAAGCAGCTGAAGATCTAAAGCAGAGGATAATTGAACATAATATTCTAGTTGTTTCAAAGTATTACTCGAGGGTTACCTTGAAGAGACTTGCAGAGCTGCTCTGTCTAAGTATCCAGGATGCTGAGAAGCATCTTTCAGAAATGGTTGTGTCCAAGGCGCTGGTGGCCAAGATTGACAGGCCAATAGGCATAATTTGTTTCCAAACTGCAAAAGATAGTAACAACGTTCTTAATTCGTGGGCTACAAACTTGGAGAAGTTGCTTGATCTTGTTGAGAAGAGCTGCCACCAAATACACAAAGAAACAATGGTTCACAAGGCAGCTCTGAAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

442

Amino Acids

50.6

Weight (kDa)

7.01

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSMD12-CSN4_N PF22241 11 - 188 4.3e-65 PSMD12/CSN4, N-terminal
PSMD12-CSN4_N PF22241 211 - 249 1.5e-09 PSMD12/CSN4, N-terminal
PCI PF01399 333 - 399 9e-17 PCI domain
RPN5_C PF18098 405 - 437 7.4e-11 26S proteasome regulatory subunit RPN5 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 122
Acc36I ACCTGC 1 cut(s) 122
AccB7I CCANNNNNTGG 2 cut(s) 163, 930
AciI CCGC 3 cut(s) 83, 410, 640
AclI AACGTT 1 cut(s) 1218
AclWI GGATC 5 cut(s) 650, 721, 740, 753, 803
AcoI YGGCCR 1 cut(s) 1158
AcsI RAATTY 1 cut(s) 871
AcuI CTGAAG 2 cut(s) 402, 1023
AfaI GTAC 2 cut(s) 618, 788
AfiI CCNNNNNNNGG 3 cut(s) 163, 581, 930
AjnI CCWGG 2 cut(s) 840, 1107
Alw21I GWGCWC 2 cut(s) 331, 508
Alw26I GTCTC 1 cut(s) 1072
AlwI GGATC 5 cut(s) 650, 721, 740, 753, 803
AlwNI CAGNNNCTG 1 cut(s) 163
Ama87I CYCGRG 1 cut(s) 1060
AoxI GGCC 2 cut(s) 1158, 1172
ApeKI GCWGC 5 cut(s) 386, 998, 1090, 1275, 1313
ApoI RAATTY 1 cut(s) 871
AseI ATTAAT 1 cut(s) 656
Asp700I GAANNNNTTC 1 cut(s) 468
AspLEI GCGC 1 cut(s) 1153
AspS9I GGNCC 2 cut(s) 214, 921
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 4 cut(s) 86, 596, 792, 1001
AvaI CYCGRG 1 cut(s) 1060
AvaII GGWCC 2 cut(s) 214, 921
BalI TGGCCA 1 cut(s) 1160
BamHI GGATCC 1 cut(s) 745
BarI GAAGNNNNNNTAC 2 cut(s) 629, 661
BbsI GAAGAC 2 cut(s) 149, 489
Bbv12I GWGCWC 2 cut(s) 331, 508
BbvI GCAGC 5 cut(s) 373, 1010, 1077, 1262, 1325
BccI CCATC 1 cut(s) 628
BceAI ACGGC 2 cut(s) 165, 687
BciT130I CCWGG 2 cut(s) 842, 1109
BciVI GTATCC 2 cut(s) 753, 1115
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 1072
BfaI CTAG 1 cut(s) 1040
BfoI RGCGCY 1 cut(s) 1154
BfuAI ACCTGC 1 cut(s) 122
BfuI GTATCC 2 cut(s) 753, 1115
BglII AGATCT 1 cut(s) 1006
BisI GCNGC 5 cut(s) 387, 999, 1091, 1276, 1314
BlsI GCNGC 5 cut(s) 388, 1000, 1092, 1277, 1315
Bme1390I CCNGG 3 cut(s) 301, 842, 1109
Bme18I GGWCC 2 cut(s) 214, 921
BmeT110I CYCGRG 1 cut(s) 1060
BmgT120I GGNCC 2 cut(s) 214, 921
BmiI GGNNCC 3 cut(s) 88, 747, 867
BmrFI CCNGG 3 cut(s) 301, 842, 1109
BmsI GCATC 4 cut(s) 13, 387, 1102, 1132
BpiI GAAGAC 2 cut(s) 149, 489
BpmI CTGGAG 1 cut(s) 863
BpuEI CTTGAG 3 cut(s) 134, 176, 713
BpuMI CCSGG 1 cut(s) 301
BsaBI GATNNNNATC 1 cut(s) 852
BsaJI CCNNGG 5 cut(s) 181, 533, 576, 840, 1146
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 581, 930
Bse3DI GCAATG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 852
BseBI CCWGG 2 cut(s) 842, 1109
BseDI CCNNGG 5 cut(s) 181, 533, 576, 840, 1146
BseGI GGATG 2 cut(s) 952, 1117
BseJI GATNNNNATC 1 cut(s) 852
BseLI CCNNNNNNNGG 3 cut(s) 163, 581, 930
BseMI GCAATG 1 cut(s) 231
BseMII CTCAG 3 cut(s) 432, 767, 1107
BseXI GCAGC 5 cut(s) 373, 1010, 1077, 1262, 1325
BshFI GGCC 2 cut(s) 1160, 1174
BsiHKAI GWGCWC 2 cut(s) 331, 508
BsiHKCI CYCGRG 1 cut(s) 1060
BsiSI CCGG 1 cut(s) 300
BslI CCNNNNNNNGG 3 cut(s) 163, 581, 930
BsmAI GTCTC 1 cut(s) 1072
BsmI GAATGC 1 cut(s) 452
BsnI GGCC 2 cut(s) 1160, 1174
BsoBI CYCGRG 1 cut(s) 1060
Bsp1286I GDGCHC 2 cut(s) 331, 508
Bsp143I GATC 9 cut(s) 238, 250, 354, 642, 726, 745, 808, 1006, 1258
BspACI CCGC 3 cut(s) 83, 410, 640
BspANI GGCC 2 cut(s) 1160, 1174
BspCNI CTCAG 3 cut(s) 433, 766, 1108
BspLI GGNNCC 3 cut(s) 88, 747, 867
BspMI ACCTGC 1 cut(s) 122
BspPI GGATC 5 cut(s) 650, 721, 740, 753, 803
BspQI GCTCTTC 2 cut(s) 357, 1265
BsrDI GCAATG 1 cut(s) 231
BssECI CCNNGG 5 cut(s) 181, 533, 576, 840, 1146
BssMI GATC 9 cut(s) 238, 250, 354, 642, 726, 745, 808, 1006, 1258
BssT1I CCWWGG 3 cut(s) 533, 576, 1146
Bst2UI CCWGG 2 cut(s) 842, 1109
Bst4CI ACNGT 4 cut(s) 333, 488, 516, 666
Bst6I CTCTTC 3 cut(s) 357, 1070, 1265
BstAPI GCANNNNNTGC 1 cut(s) 226
BstC8I GCNNGC 2 cut(s) 198, 222
BstDEI CTNAG 5 cut(s) 19, 441, 753, 1100, 1116
BstDSI CCRYGG 1 cut(s) 181
BstEII GGTNACC 1 cut(s) 1066
BstF5I GGATG 2 cut(s) 952, 1117
BstH2I RGCGCY 1 cut(s) 1154
BstHHI GCGC 1 cut(s) 1153
BstKTI GATC 9 cut(s) 241, 253, 357, 645, 729, 748, 811, 1009, 1261
BstMAI GTCTC 1 cut(s) 1072
BstMBI GATC 9 cut(s) 238, 250, 354, 642, 726, 745, 808, 1006, 1258
BstMWI GCNNNNNNNGC 4 cut(s) 206, 226, 386, 1157
BstNI CCWGG 2 cut(s) 842, 1109
BstNSI RCATGY 1 cut(s) 973
BstPI GGTNACC 1 cut(s) 1066
BstSCI CCNGG 3 cut(s) 299, 840, 1107
BstV1I GCAGC 5 cut(s) 373, 1010, 1077, 1262, 1325
BstV2I GAAGAC 2 cut(s) 149, 489
BstX2I RGATCY 4 cut(s) 642, 726, 745, 1006
BstXI CCANNNNNNTGG 2 cut(s) 756, 1153
BstYI RGATCY 4 cut(s) 642, 726, 745, 1006
BsuI GTATCC 2 cut(s) 753, 1115
BsuRI GGCC 2 cut(s) 1160, 1174
BtgI CCRYGG 1 cut(s) 181
BtgZI GCGATG 1 cut(s) 606
BtsCI GGATG 2 cut(s) 952, 1117
BtsIMutI CAGTG 2 cut(s) 102, 761
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 2 cut(s) 198, 222
CaiI CAGNNNCTG 1 cut(s) 163
CfoI GCGC 1 cut(s) 1153
Cfr13I GGNCC 2 cut(s) 214, 921
Csp6I GTAC 2 cut(s) 617, 787
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviAII CATG 1 cut(s) 970
CviQI GTAC 2 cut(s) 617, 787
DdeI CTNAG 5 cut(s) 19, 441, 753, 1100, 1116
DpnI GATC 9 cut(s) 240, 252, 356, 644, 728, 747, 810, 1008, 1260
DpnII GATC 9 cut(s) 238, 250, 354, 642, 726, 745, 808, 1006, 1258
DraI TTTAAA 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 1158
Eam1104I CTCTTC 3 cut(s) 357, 1070, 1265
EarI CTCTTC 3 cut(s) 357, 1070, 1265
Eco130I CCWWGG 3 cut(s) 533, 576, 1146
Eco47I GGWCC 2 cut(s) 214, 921
Eco57I CTGAAG 2 cut(s) 402, 1023
Eco88I CYCGRG 1 cut(s) 1060
Eco91I GGTNACC 1 cut(s) 1066
EcoO65I GGTNACC 1 cut(s) 1066
EcoRII CCWGG 2 cut(s) 840, 1107
EcoT14I CCWWGG 3 cut(s) 533, 576, 1146
ErhI CCWWGG 3 cut(s) 533, 576, 1146
FaeI CATG 1 cut(s) 973
FatI CATG 1 cut(s) 969
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 5 cut(s) 387, 999, 1091, 1276, 1314
FokI GGATG 2 cut(s) 959, 1124
Fsp4HI GCNGC 5 cut(s) 387, 999, 1091, 1276, 1314
FspBI CTAG 1 cut(s) 1040
GlaI GCGC 1 cut(s) 1152
GluI GCNGC 5 cut(s) 387, 999, 1091, 1276, 1314
GsuI CTGGAG 1 cut(s) 863
HaeII RGCGCY 1 cut(s) 1154
HaeIII GGCC 2 cut(s) 1160, 1174
HapII CCGG 1 cut(s) 300
HhaI GCGC 1 cut(s) 1153
Hin1II CATG 1 cut(s) 973
Hin6I GCGC 1 cut(s) 1151
HinP1I GCGC 1 cut(s) 1151
HinfI GANTC 2 cut(s) 160, 853
HpaII CCGG 1 cut(s) 300
HphI GGTGA 4 cut(s) 86, 596, 792, 1001
Hpy166II GTNNAC 1 cut(s) 1306
Hpy188I TCNGA 4 cut(s) 243, 862, 1132, 1320
Hpy188III TCNNGA 5 cut(s) 113, 254, 461, 491, 931
Hpy8I GTNNAC 1 cut(s) 1306
HpyAV CCTTC 5 cut(s) 88, 575, 741, 836, 1315
HpyCH4III ACNGT 4 cut(s) 333, 488, 516, 666
HpyCH4IV ACGT 1 cut(s) 1218
HpyCH4V TGCA 8 cut(s) 209, 229, 350, 400, 452, 817, 1085, 1202
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 226, 386, 1157
HpyF3I CTNAG 5 cut(s) 19, 441, 753, 1100, 1116
HpySE526I ACGT 1 cut(s) 1218
Hsp92II CATG 1 cut(s) 973
HspAI GCGC 1 cut(s) 1151
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 9 cut(s) 238, 250, 354, 642, 726, 745, 808, 1006, 1258
LguI GCTCTTC 2 cut(s) 357, 1265
LmnI GCTCC 1 cut(s) 610
Lsp1109I GCAGC 5 cut(s) 373, 1010, 1077, 1262, 1325
LweI GCATC 4 cut(s) 13, 387, 1102, 1132
MaeI CTAG 1 cut(s) 1040
MaeII ACGT 1 cut(s) 1218
MaeIII GTNAC 9 cut(s) 14, 100, 202, 798, 879, 898, 989, 1066, 1211
MalI GATC 9 cut(s) 240, 252, 356, 644, 728, 747, 810, 1008, 1260
MboI GATC 9 cut(s) 238, 250, 354, 642, 726, 745, 808, 1006, 1258
MflI RGATCY 4 cut(s) 642, 726, 745, 1006
MhlI GDGCHC 2 cut(s) 331, 508
MlsI TGGCCA 1 cut(s) 1160
MluCI AATT 8 cut(s) 38, 318, 653, 832, 871, 1023, 1185, 1225
MluNI TGGCCA 1 cut(s) 1160
Mox20I TGGCCA 1 cut(s) 1160
MroXI GAANNNNTTC 1 cut(s) 468
MscI TGGCCA 1 cut(s) 1160
MseI TTAA 8 cut(s) 126, 276, 335, 656, 738, 831, 942, 1224
MslI CAYNNNNRTG 1 cut(s) 681
Msp20I TGGCCA 1 cut(s) 1160
MspA1I CMGCKG 1 cut(s) 1001
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 3 cut(s) 301, 842, 1109
Mva1269I GAATGC 1 cut(s) 452
MvaI CCWGG 2 cut(s) 842, 1109
MwoI GCNNNNNNNGC 4 cut(s) 206, 226, 386, 1157
NciI CCSGG 1 cut(s) 301
NdeII GATC 9 cut(s) 238, 250, 354, 642, 726, 745, 808, 1006, 1258
NlaIII CATG 1 cut(s) 973
NlaIV GGNNCC 3 cut(s) 88, 747, 867
NmuCI GTSAC 4 cut(s) 14, 100, 798, 989
NspI RCATGY 1 cut(s) 973
PaeR7I CTCGAG 1 cut(s) 1060
PaqCI CACCTGC 1 cut(s) 122
PciSI GCTCTTC 2 cut(s) 357, 1265
PctI GAATGC 1 cut(s) 452
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 2 cut(s) 160, 853
PflMI CCANNNNNTGG 2 cut(s) 163, 930
PfoI TCCNGGA 1 cut(s) 1107
PkrI GCNGC 5 cut(s) 388, 1000, 1092, 1277, 1315
PshBI ATTAAT 1 cut(s) 656
Psp1406I AACGTT 1 cut(s) 1218
Psp6I CCWGG 2 cut(s) 840, 1107
PspEI GGTNACC 1 cut(s) 1066
PspGI CCWGG 2 cut(s) 840, 1107
PspN4I GGNNCC 3 cut(s) 88, 747, 867
PspPI GGNCC 2 cut(s) 214, 921
PspXI VCTCGAGB 1 cut(s) 1060
PstNI CAGNNNCTG 1 cut(s) 163
PsuI RGATCY 4 cut(s) 642, 726, 745, 1006
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 2 cut(s) 618, 788
RsaNI GTAC 2 cut(s) 617, 787
RseI CAYNNNNRTG 1 cut(s) 681
SapI GCTCTTC 2 cut(s) 357, 1265
SaqAI TTAA 8 cut(s) 126, 276, 335, 656, 738, 831, 942, 1224
SatI GCNGC 5 cut(s) 387, 999, 1091, 1276, 1314
Sau3AI GATC 9 cut(s) 238, 250, 354, 642, 726, 745, 808, 1006, 1258
Sau96I GGNCC 2 cut(s) 214, 921
ScrFI CCNGG 3 cut(s) 301, 842, 1109
SduI GDGCHC 2 cut(s) 331, 508
SfaNI GCATC 4 cut(s) 13, 387, 1102, 1132
Sfr274I CTCGAG 1 cut(s) 1060
SinI GGWCC 2 cut(s) 214, 921
SlaI CTCGAG 1 cut(s) 1060
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 4 cut(s) 113, 191, 692, 1060
SmoI CTYRAG 4 cut(s) 113, 191, 692, 1060
Sse9I AATT 8 cut(s) 38, 318, 653, 832, 871, 1023, 1185, 1225
SsiI CCGC 3 cut(s) 83, 410, 640
SspI AATATT 1 cut(s) 1036
SspMI CTAG 1 cut(s) 1040
StyD4I CCNGG 3 cut(s) 299, 840, 1107
StyI CCWWGG 3 cut(s) 533, 576, 1146
TaaI ACNGT 4 cut(s) 333, 488, 516, 666
TaiI ACGT 1 cut(s) 1221
TaqI TCGA 2 cut(s) 518, 1061
TasI AATT 8 cut(s) 38, 318, 653, 832, 871, 1023, 1185, 1225
TfiI GAWTC 2 cut(s) 160, 853
Tru1I TTAA 8 cut(s) 126, 276, 335, 656, 738, 831, 942, 1224
Tru9I TTAA 8 cut(s) 126, 276, 335, 656, 738, 831, 942, 1224
TscAI CASTG 2 cut(s) 109, 761
TseFI GTSAC 4 cut(s) 14, 100, 798, 989
TseI GCWGC 5 cut(s) 386, 998, 1090, 1275, 1313
Tsp45I GTSAC 4 cut(s) 14, 100, 798, 989
TspDTI ATGAA 2 cut(s) 445, 666
TspRI CASTG 2 cut(s) 109, 761
Van91I CCANNNNNTGG 2 cut(s) 163, 930
VpaK11BI GGWCC 2 cut(s) 214, 921
VspI ATTAAT 1 cut(s) 656
XapI RAATTY 1 cut(s) 871
XceI RCATGY 1 cut(s) 973
XcmI CCANNNNNNNNNTGG 1 cut(s) 1153
XhoI CTCGAG 1 cut(s) 1060
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 1 cut(s) 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.