pycom10g07060

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003043
Physical Location & Seq
Reverse (-)
8260962 .. 8261971
1010 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g07060.1

Sequence Viewer

Length: 348 bp
ATGGATTCACAGACCCAGAACACCTCGTTGCAGAGACTCCAAAATGTGGAGAATAGAATCGTTAAGGTTTTGGAGCTAGCAGGAGGCGTCATGGACGAGCTTGCAAACCCTAGCGGTCCCAGAAAGGAGTTCATCAACAACCATTGCCGCGAGTTCATGCAATTGATCAAGGATATTCAAGTGGCACTACGGGATGAAATTAAAAGTGCATGCGATTACCGTCCATTTGAGAAGTGTGATTACAGTTCAAGAATAGCTAATGAGATCTGTTGCAAGAAGCTTGAGTATGTCATGTCACAATTGGATCGAATGAAAGAAACAATTGATGAGTATCATAATGCAGTTTGA

Protein Analysis

116

Amino Acids

13.4

Weight (kDa)

5.41

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med11 PF10280 8 - 109 3.6e-21 Mediator complex protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 46
AccII CGCG 1 cut(s) 150
AciI CCGC 2 cut(s) 114, 148
AclWI GGATC 1 cut(s) 312
AcyI GRCGYC 1 cut(s) 87
AfiI CCNNNNNNNGG 1 cut(s) 46
AgsI TTSAA 2 cut(s) 179, 249
AluBI AGCT 4 cut(s) 76, 100, 257, 280
AluI AGCT 4 cut(s) 76, 100, 257, 280
Alw26I GTCTC 1 cut(s) 28
AlwI GGATC 1 cut(s) 312
AspS9I GGNCC 1 cut(s) 116
AsuNHI GCTAGC 1 cut(s) 76
AvaII GGWCC 1 cut(s) 116
BarI GAAGNNNNNNTAC 2 cut(s) 224, 256
BclI TGATCA 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 28
BfaI CTAG 2 cut(s) 77, 111
BglII AGATCT 1 cut(s) 264
BisI GCNGC 1 cut(s) 148
BlsI GCNGC 1 cut(s) 149
Bme18I GGWCC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 118
BmtI GCTAGC 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 302
BsaBI GATNNNNATC 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 87
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse3DI GCAATG 1 cut(s) 142
Bse8I GATNNNNATC 1 cut(s) 330
BseGI GGATG 1 cut(s) 199
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 102
Bsp143I GATC 3 cut(s) 165, 264, 304
BspACI CCGC 2 cut(s) 114, 148
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 118
BspOI GCTAGC 1 cut(s) 80
BspPI GGATC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 142
BssMI GATC 3 cut(s) 165, 264, 304
BssNI GRCGYC 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 221, 245
BstACI GRCGYC 1 cut(s) 87
BstC8I GCNNGC 3 cut(s) 78, 102, 211
BstF5I GGATG 1 cut(s) 199
BstFNI CGCG 1 cut(s) 150
BstKTI GATC 3 cut(s) 168, 267, 307
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 3 cut(s) 165, 264, 304
BstNSI RCATGY 1 cut(s) 213
BstUI CGCG 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 264
BstYI RGATCY 1 cut(s) 264
BtsCI GGATG 1 cut(s) 199
Cac8I GCNNGC 3 cut(s) 78, 102, 211
Cfr13I GGNCC 1 cut(s) 116
CseI GACGC 1 cut(s) 76
CviAII CATG 4 cut(s) 91, 157, 210, 292
CviJI RGCY 4 cut(s) 76, 100, 257, 280
CviKI_1 RGCY 4 cut(s) 76, 100, 257, 280
DpnI GATC 3 cut(s) 167, 266, 306
DpnII GATC 3 cut(s) 165, 264, 304
Eco47I GGWCC 1 cut(s) 116
FaeI CATG 4 cut(s) 94, 160, 213, 295
FaiI YATR 6 cut(s) 92, 158, 211, 288, 293, 336
FaqI GGGAC 1 cut(s) 102
FatI CATG 4 cut(s) 90, 156, 209, 291
FbaI TGATCA 1 cut(s) 165
Fnu4HI GCNGC 1 cut(s) 148
FokI GGATG 1 cut(s) 206
Fsp4HI GCNGC 1 cut(s) 148
FspBI CTAG 2 cut(s) 77, 111
GluI GCNGC 1 cut(s) 148
HgaI GACGC 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 4 cut(s) 94, 160, 213, 295
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 3 cut(s) 5, 36, 57
Hpy188III TCNNGA 1 cut(s) 249
HpyCH4III ACNGT 2 cut(s) 221, 245
HpyCH4V TGCA 6 cut(s) 31, 104, 160, 209, 273, 341
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 4 cut(s) 94, 160, 213, 295
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 3 cut(s) 165, 264, 304
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 3 cut(s) 29, 66, 133
MaeI CTAG 2 cut(s) 77, 111
MaeIII GTNAC 1 cut(s) 294
MalI GATC 3 cut(s) 167, 266, 306
MboI GATC 3 cut(s) 165, 264, 304
MfeI CAATTG 3 cut(s) 161, 299, 321
MflI RGATCY 1 cut(s) 264
MluCI AATT 4 cut(s) 161, 198, 299, 321
MlyI GAGTC 1 cut(s) 30
MnlI CCTC 2 cut(s) 34, 77
MseI TTAA 2 cut(s) 63, 201
MunI CAATTG 3 cut(s) 161, 299, 321
MvnI CGCG 1 cut(s) 150
NdeII GATC 3 cut(s) 165, 264, 304
NheI GCTAGC 1 cut(s) 76
NlaIII CATG 4 cut(s) 94, 160, 213, 295
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 294
NspI RCATGY 1 cut(s) 213
PaeI GCATGC 1 cut(s) 213
PfeI GAWTC 2 cut(s) 5, 57
PflMI CCANNNNNTGG 1 cut(s) 46
PkrI GCNGC 1 cut(s) 149
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 116
PsuI RGATCY 1 cut(s) 264
SaqAI TTAA 2 cut(s) 63, 201
SatI GCNGC 1 cut(s) 148
Sau3AI GATC 3 cut(s) 165, 264, 304
Sau96I GGNCC 1 cut(s) 116
SchI GAGTC 1 cut(s) 30
SetI ASST 6 cut(s) 26, 69, 78, 102, 259, 282
SinI GGWCC 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SphI GCATGC 1 cut(s) 213
Sse9I AATT 4 cut(s) 161, 198, 299, 321
SsiI CCGC 2 cut(s) 114, 148
SspMI CTAG 2 cut(s) 77, 111
TaaI ACNGT 2 cut(s) 221, 245
TaqI TCGA 1 cut(s) 307
TasI AATT 4 cut(s) 161, 198, 299, 321
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 2 cut(s) 5, 57
Tru1I TTAA 2 cut(s) 63, 201
Tru9I TTAA 2 cut(s) 63, 201
TseFI GTSAC 1 cut(s) 294
Tsp45I GTSAC 1 cut(s) 294
TspDTI ATGAA 4 cut(s) 121, 145, 210, 326
Van91I CCANNNNNTGG 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 116
XceI RCATGY 1 cut(s) 213
XspI CTAG 2 cut(s) 77, 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.