pycom10g14480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006631
Physical Location & Seq
Forward (+)
17875683 .. 17877974
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1725 bp
ATGAAGAAACCCACTCTCAGCAATCCCTGGGGCTGCGCAGCCGCTGGGAGTAGCCGTCGCCATCTTTCTTCTCCGATCAAAAAATTCTCCAAGATTAGGTTCTTCCCCCAGAACCTCTTCAACCCCTACAATTTGATCAAGTTCGTCCAATCTGACTTCTTCCTTAATGAGCAGCTTCAGGTCCACAAGGCCTCAATTCCCGTGGCAGCGACCCTCACGGCTCAGAACTACGGCGGAGGTGAAGTGGCAGCAATCGAGAAGCTTGCGTCGGACGCGGTCGACGACTTCGTCGACTGGCAGGTGGTCGGCGCCGGAGAAGATGACCTGGTGATGTCGGGCGGCGAGCCGATGCCGAGGGTTGTCTTCAACGCTGCGCCGAGCTTCAAGGAGGCCAAAGAGGCCACCGCTGAATTGAAAGACGCCCTCGATAAGGTGTATCTTTCTTTGCCCAAATCTACTGATTTGGGGGAGCCATCTGCTGCTGCTGGTGCGGCTGGGCTGCCACTGATAAAAAACCCTAAACCTGAGGAGATGGGATCCCTACTTCCTACTAGGACTTCAGTGCCGAAACATGTTCTTCGAGCATCTGAAATGTTGAGTAGAAGTTCTGAAGCTCAGAATGTTGTTGCTTCAATTGCAAGTGACCCAAATGCCTGGAATGCTATGATGGAAAATTCTGCGGTCAAGCAATTTATGGCCAACAAGAATTGTAATCTTAACACAGAAGAGTATGCTGCAGATACTTATCCAGCTTCACCCAAGAAGCTTGAAGATGAATCAGAGAAATCTAACAGCTTGGAAGATATGCTCGGTGGTTTTGTGCAGAAAATCAAGCTTACTGTGGATAAGTGGGTCAATGATTTGTCGTCATTCATCCAGAACATTTTCGAATTCCCCTGCTCGCTGATCCTCTACTTCAACAACTCCGTCTTCTTCGTCTCTGCTGTTGACGTCGACAAGTTTCTCCAGTATGGCAATTCGTTGAAAGCTAGCAACTATAGATCTGCCAGCACACCGATGACTGCAATCTCCTCGGAAAATTGGCCGAAGACCACAAAGGGCAACTACAAGCTCACTGATGCCGTTCTTCCTGAATCGAAAAACTGGAAAGAAGAAGGCATAGTGATTCCAGTTAAAGATCAAGGTCACTGTGGATCTTGCTGGACATTCAGCACCACTGGAGCTCTCGAGGCTGCGTATGTGCAGGAATTCGGAAAGCAAATCTCCCTATCGGAGCAGCAGCTTGTGGATTGCGCTGGCGCTTTCAACAATAACGGCTGCAGTGGTGGGTTGCCATCTCAAGCATTTAAGTACATCAAGTACAATGGCGGTCTTGACACCGACGCTGCTTATCCGTATGTTGGAGTCGACGGTGTTTACAAATTTGGCACCATCTCATTGGGTGTTTTAGCTTCCTCTTCATGCTCTCCTTGTCAAGCTCACTTCTCTCCTCACTCCGGCCACCGTGGTCAAAATCGGCGAAGAACTTCAGTGACCGCTGTCAAACGACTGGCCGATCGTGAAGCTCGACGCTTTGCACTACCTCCAAGCTCGACTCCAGCTCTCTGTCCGTCTTCGTCCTCCTCTGCGAGCCTTCTCCTTCTACCGATCAGCTTCAGCAGCACGGACACCCCTTCCTCCTCCGTTTTCTCTCGAACTCGGCCTGGGTTTTCACCCACAAACCTAGCTTGCAGAGCACCATATATATATAAAAAAATAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

575

Amino Acids

62.22

Weight (kDa)

8.32

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C1 PF00112 363 - 476 1.1e-34 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 289
AasI GACNNNNNNGTC 1 cut(s) 287
AatII GACGTC 1 cut(s) 954
Acc16I TGCGCA 1 cut(s) 37
Acc36I ACCTGC 1 cut(s) 289
AccB1I GGYRCC 2 cut(s) 308, 1388
AccI GTMKAC 4 cut(s) 279, 291, 954, 1368
AccII CGCG 1 cut(s) 275
AciI CCGC 9 cut(s) 42, 234, 275, 339, 405, 491, 680, 1329, 1497
AclWI GGATC 4 cut(s) 531, 544, 901, 1162
AcoI YGGCCR 4 cut(s) 696, 1043, 1459, 1512
AcsI RAATTY 5 cut(s) 83, 673, 890, 1208, 1382
AcuI CTGAAG 5 cut(s) 161, 543, 630, 1473, 1600
AcyI GRCGYC 3 cut(s) 309, 420, 951
AfaI GTAC 2 cut(s) 1313, 1322
AfiI CCNNNNNNNGG 4 cut(s) 96, 430, 1361, 1457
AflIII ACRYGT 1 cut(s) 571
AgsI TTSAA 9 cut(s) 121, 367, 385, 415, 633, 770, 919, 985, 1267
AjnI CCWGG 4 cut(s) 26, 324, 653, 1663
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
Alw21I GWGCWC 2 cut(s) 1186, 1699
Alw26I GTCTC 1 cut(s) 943
AlwI GGATC 4 cut(s) 531, 544, 901, 1162
AlwNI CAGNNNCTG 1 cut(s) 44
Ama87I CYCGRG 1 cut(s) 1187
AoxI GGCC 8 cut(s) 189, 390, 399, 696, 1043, 1459, 1512, 1661
ApoI RAATTY 5 cut(s) 83, 673, 890, 1208, 1382
Asp700I GAANNNNTTC 3 cut(s) 116, 884, 1486
AspLEI GCGC 5 cut(s) 38, 311, 376, 1256, 1262
AspS9I GGNCC 1 cut(s) 181
AsuHPI GGTGA 4 cut(s) 251, 340, 747, 1665
AsuII TTCGAA 1 cut(s) 888
AsuNHI GCTAGC 1 cut(s) 989
AvaI CYCGRG 1 cut(s) 1187
AvaII GGWCC 1 cut(s) 181
AxyI CCTNAGG 1 cut(s) 525
BalI TGGCCA 1 cut(s) 698
BamHI GGATCC 1 cut(s) 536
BanI GGYRCC 2 cut(s) 308, 1388
BanII GRGCYC 1 cut(s) 1186
BbsI GAAGAC 4 cut(s) 355, 922, 1055, 1566
Bbv12I GWGCWC 2 cut(s) 1186, 1699
BccI CCATC 6 cut(s) 69, 481, 526, 661, 1303, 1400
BceAI ACGGC 5 cut(s) 39, 234, 247, 1067, 1291
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 1014, 1048
BciT130I CCWGG 4 cut(s) 28, 326, 655, 1665
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 943
BfaI CTAG 3 cut(s) 552, 990, 1685
BfmI CTRYAG 3 cut(s) 735, 997, 1279
BfoI RGCGCY 2 cut(s) 312, 1263
BfuAI ACCTGC 1 cut(s) 289
BglI GCCNNNNNGGC 1 cut(s) 398
BglII AGATCT 1 cut(s) 1001
Bme1390I CCNGG 4 cut(s) 28, 326, 655, 1665
Bme18I GGWCC 1 cut(s) 181
BmeT110I CYCGRG 1 cut(s) 1187
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 4 cut(s) 310, 471, 538, 1390
BmrFI CCNGG 4 cut(s) 28, 326, 655, 1665
BmsI GCATC 3 cut(s) 339, 593, 1069
BmtI GCTAGC 1 cut(s) 993
BoxI GACNNNNGTC 1 cut(s) 1499
BpiI GAAGAC 4 cut(s) 355, 922, 1055, 1566
BpmI CTGGAG 3 cut(s) 950, 1200, 1542
Bpu14I TTCGAA 1 cut(s) 888
BpuEI CTTGAG 1 cut(s) 1284
BsaBI GATNNNNATC 1 cut(s) 744
BsaHI GRCGYC 3 cut(s) 309, 420, 951
BsaJI CCNNGG 7 cut(s) 26, 27, 201, 353, 1032, 1465, 1664
BsaXI ACNNNNNCTCC 4 cut(s) 40, 70, 228, 258
Bsc4I CCNNNNNNNGG 4 cut(s) 96, 430, 1361, 1457
Bse1I ACTGG 6 cut(s) 299, 967, 1109, 1130, 1183, 1515
Bse21I CCTNAGG 1 cut(s) 525
Bse8I GATNNNNATC 1 cut(s) 744
BseBI CCWGG 4 cut(s) 28, 326, 655, 1665
BseDI CCNNGG 7 cut(s) 26, 27, 201, 353, 1032, 1465, 1664
BseGI GGATG 1 cut(s) 873
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 4 cut(s) 96, 430, 1361, 1457
BseMII CTCAG 4 cut(s) 31, 236, 516, 629
BseNI ACTGG 6 cut(s) 299, 967, 1109, 1130, 1183, 1515
BseRI GAGGAG 5 cut(s) 542, 1021, 1440, 1573, 1630
BseYI CCCAGC 2 cut(s) 44, 494
BsgI GTGCAG 2 cut(s) 842, 1223
Bsh1236I CGCG 1 cut(s) 275
Bsh1285I CGRYCG 2 cut(s) 279, 1519
BshFI GGCC 8 cut(s) 191, 392, 401, 698, 1045, 1461, 1514, 1663
BshNI GGYRCC 2 cut(s) 308, 1388
BsiEI CGRYCG 2 cut(s) 279, 1519
BsiHKAI GWGCWC 2 cut(s) 1186, 1699
BsiHKCI CYCGRG 1 cut(s) 1187
BsiSI CCGG 2 cut(s) 312, 1458
BslI CCNNNNNNNGG 4 cut(s) 96, 430, 1361, 1457
BsmAI GTCTC 1 cut(s) 943
BsmBI CGTCTC 1 cut(s) 943
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 8 cut(s) 191, 392, 401, 698, 1045, 1461, 1514, 1663
BsoBI CYCGRG 1 cut(s) 1187
Bsp119I TTCGAA 1 cut(s) 888
Bsp1286I GDGCHC 2 cut(s) 1186, 1699
Bsp143I GATC 9 cut(s) 75, 135, 536, 906, 1001, 1138, 1154, 1516, 1608
BspACI CCGC 9 cut(s) 42, 234, 275, 339, 405, 491, 680, 1329, 1497
BspANI GGCC 8 cut(s) 191, 392, 401, 698, 1045, 1461, 1514, 1663
BspCNI CTCAG 4 cut(s) 30, 235, 517, 628
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 4 cut(s) 310, 471, 538, 1390
BspMAI CTGCAG 2 cut(s) 739, 1283
BspMI ACCTGC 1 cut(s) 289
BspOI GCTAGC 1 cut(s) 993
BspPI GGATC 4 cut(s) 531, 544, 901, 1162
BspT104I TTCGAA 1 cut(s) 888
BspT107I GGYRCC 2 cut(s) 308, 1388
BsrI ACTGG 6 cut(s) 299, 967, 1109, 1130, 1183, 1515
BssECI CCNNGG 7 cut(s) 26, 27, 201, 353, 1032, 1465, 1664
BssMI GATC 9 cut(s) 75, 135, 536, 906, 1001, 1138, 1154, 1516, 1608
BssNI GRCGYC 3 cut(s) 309, 420, 951
Bst2UI CCWGG 4 cut(s) 28, 326, 655, 1665
Bst4CI ACNGT 4 cut(s) 841, 1151, 1373, 1466
Bst6I CTCTTC 3 cut(s) 122, 720, 1423
BstACI GRCGYC 3 cut(s) 309, 420, 951
BstBI TTCGAA 1 cut(s) 888
BstC8I GCNNGC 8 cut(s) 264, 344, 902, 991, 1009, 1258, 1591, 1690
BstDEI CTNAG 4 cut(s) 17, 222, 525, 615
BstDSI CCRYGG 2 cut(s) 201, 1465
BstENI CCTNNNNNAGG 1 cut(s) 428
BstF5I GGATG 1 cut(s) 873
BstFNI CGCG 1 cut(s) 275
BstH2I RGCGCY 2 cut(s) 312, 1263
BstHHI GCGC 5 cut(s) 38, 311, 376, 1256, 1262
BstKTI GATC 9 cut(s) 78, 138, 539, 909, 1004, 1141, 1157, 1519, 1611
BstMAI GTCTC 1 cut(s) 943
BstMBI GATC 9 cut(s) 75, 135, 536, 906, 1001, 1138, 1154, 1516, 1608
BstMCI CGRYCG 2 cut(s) 279, 1519
BstMWI GCNNNNNNNGC 9 cut(s) 272, 398, 488, 491, 635, 659, 1190, 1620, 1694
BstNI CCWGG 4 cut(s) 28, 326, 655, 1665
BstNSI RCATGY 1 cut(s) 575
BstPAI GACNNNNGTC 1 cut(s) 1499
BstSCI CCNGG 4 cut(s) 26, 324, 653, 1663
BstSFI CTRYAG 3 cut(s) 735, 997, 1279
BstUI CGCG 1 cut(s) 275
BstV2I GAAGAC 4 cut(s) 355, 922, 1055, 1566
BstX2I RGATCY 3 cut(s) 536, 1001, 1154
BstXI CCANNNNNNTGG 2 cut(s) 654, 1399
BstYI RGATCY 3 cut(s) 536, 1001, 1154
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 8 cut(s) 191, 392, 401, 698, 1045, 1461, 1514, 1663
BtgI CCRYGG 2 cut(s) 201, 1465
BtsCI GGATG 1 cut(s) 873
BtsI GCAGTG 1 cut(s) 1288
BtsIMutI CAGTG 7 cut(s) 503, 567, 1074, 1147, 1176, 1288, 1497
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 8 cut(s) 264, 344, 902, 991, 1009, 1258, 1591, 1690
CaiI CAGNNNCTG 1 cut(s) 44
CfoI GCGC 5 cut(s) 38, 311, 376, 1256, 1262
Cfr13I GGNCC 1 cut(s) 181
CseI GACGC 5 cut(s) 255, 281, 428, 1352, 1539
CsiI ACCWGGT 1 cut(s) 324
Csp6I GTAC 2 cut(s) 1312, 1321
CspCI CAANNNNNGTGG 2 cut(s) 183, 218
CviAII CATG 2 cut(s) 572, 1422
CviQI GTAC 2 cut(s) 1312, 1321
DdeI CTNAG 4 cut(s) 17, 222, 525, 615
DinI GGCGCC 1 cut(s) 310
DpnI GATC 9 cut(s) 77, 137, 538, 908, 1003, 1140, 1156, 1518, 1610
DpnII GATC 9 cut(s) 75, 135, 536, 906, 1001, 1138, 1154, 1516, 1608
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 4 cut(s) 696, 1043, 1459, 1512
Eam1104I CTCTTC 3 cut(s) 122, 720, 1423
EarI CTCTTC 3 cut(s) 122, 720, 1423
EciI GGCGGA 1 cut(s) 249
Ecl136II GAGCTC 1 cut(s) 1184
Eco147I AGGCCT 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 1186
Eco47I GGWCC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 1184
Eco57I CTGAAG 5 cut(s) 161, 543, 630, 1473, 1600
Eco81I CCTNAGG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 1187
EcoICRI GAGCTC 1 cut(s) 1184
EcoNI CCTNNNNNAGG 1 cut(s) 428
EcoRI GAATTC 2 cut(s) 890, 1208
EcoRII CCWGG 4 cut(s) 26, 324, 653, 1663
EcoT38I GRGCYC 1 cut(s) 1186
EgeI GGCGCC 1 cut(s) 310
EheI GGCGCC 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 943
FaeI CATG 2 cut(s) 575, 1425
FatI CATG 2 cut(s) 571, 1421
FbaI TGATCA 1 cut(s) 135
FblI GTMKAC 4 cut(s) 279, 291, 954, 1368
FokI GGATG 1 cut(s) 860
FriOI GRGCYC 1 cut(s) 1186
FspBI CTAG 3 cut(s) 552, 990, 1685
FspI TGCGCA 1 cut(s) 37
GlaI GCGC 5 cut(s) 37, 310, 375, 1255, 1261
GsaI CCCAGC 2 cut(s) 48, 498
GsuI CTGGAG 3 cut(s) 950, 1200, 1542
HaeII RGCGCY 2 cut(s) 312, 1263
HaeIII GGCC 8 cut(s) 191, 392, 401, 698, 1045, 1461, 1514, 1663
HapII CCGG 2 cut(s) 312, 1458
HgaI GACGC 5 cut(s) 255, 281, 428, 1352, 1539
HhaI GCGC 5 cut(s) 38, 311, 376, 1256, 1262
Hin1I GRCGYC 3 cut(s) 309, 420, 951
Hin1II CATG 2 cut(s) 575, 1425
Hin6I GCGC 5 cut(s) 36, 309, 374, 1254, 1260
HinP1I GCGC 5 cut(s) 36, 309, 374, 1254, 1260
HincII GTYRAC 5 cut(s) 280, 292, 949, 955, 1369
HindII GTYRAC 5 cut(s) 280, 292, 949, 955, 1369
HindIII AAGCTT 3 cut(s) 260, 764, 833
HinfI GANTC 5 cut(s) 776, 1094, 1126, 1365, 1555
HpaII CCGG 2 cut(s) 312, 1458
HphI GGTGA 4 cut(s) 251, 340, 747, 1665
Hpy166II GTNNAC 7 cut(s) 184, 280, 292, 949, 955, 1369, 1378
Hpy188III TCNNGA 7 cut(s) 256, 877, 1091, 1187, 1334, 1520, 1653
Hpy8I GTNNAC 7 cut(s) 184, 280, 292, 949, 955, 1369, 1378
Hpy99I CGWCG 8 cut(s) 60, 271, 284, 293, 956, 1346, 1373, 1533
HpyAV CCTTC 4 cut(s) 1109, 1604, 1610, 1644
HpyCH4III ACNGT 4 cut(s) 841, 1151, 1373, 1466
HpyCH4IV ACGT 1 cut(s) 951
HpyCH4V TGCA 8 cut(s) 638, 737, 823, 1025, 1204, 1281, 1538, 1692
HpyF10VI GCNNNNNNNGC 9 cut(s) 272, 398, 488, 491, 635, 659, 1190, 1620, 1694
HpyF3I CTNAG 4 cut(s) 17, 222, 525, 615
HpySE526I ACGT 1 cut(s) 951
Hsp92I GRCGYC 3 cut(s) 309, 420, 951
Hsp92II CATG 2 cut(s) 575, 1425
HspAI GCGC 5 cut(s) 36, 309, 374, 1254, 1260
KasI GGCGCC 1 cut(s) 308
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 9 cut(s) 75, 135, 536, 906, 1001, 1138, 1154, 1516, 1608
LmnI GCTCC 3 cut(s) 469, 1181, 1234
LweI GCATC 3 cut(s) 339, 593, 1069
MabI ACCWGGT 1 cut(s) 324
MaeI CTAG 3 cut(s) 552, 990, 1685
MaeII ACGT 1 cut(s) 951
MaeIII GTNAC 3 cut(s) 641, 1145, 1492
MalI GATC 9 cut(s) 77, 137, 538, 908, 1003, 1140, 1156, 1518, 1610
MboI GATC 9 cut(s) 75, 135, 536, 906, 1001, 1138, 1154, 1516, 1608
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 3 cut(s) 536, 1001, 1154
MhlI GDGCHC 2 cut(s) 1186, 1699
MlsI TGGCCA 1 cut(s) 698
MluNI TGGCCA 1 cut(s) 698
Mly113I GGCGCC 1 cut(s) 309
MlyI GAGTC 2 cut(s) 1374, 1549
MmeI TCCRAC 2 cut(s) 249, 1342
Mox20I TGGCCA 1 cut(s) 698
MroXI GAANNNNTTC 3 cut(s) 116, 884, 1486
MscI TGGCCA 1 cut(s) 698
MseI TTAA 4 cut(s) 165, 717, 1134, 1308
MslI CAYNNNNRTG 1 cut(s) 1016
Msp20I TGGCCA 1 cut(s) 698
MspA1I CMGCKG 3 cut(s) 44, 407, 1499
MspI CCGG 2 cut(s) 312, 1458
MspR9I CCNGG 4 cut(s) 28, 326, 655, 1665
MunI CAATTG 1 cut(s) 633
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 4 cut(s) 28, 326, 655, 1665
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 9 cut(s) 272, 398, 488, 491, 635, 659, 1190, 1620, 1694
NarI GGCGCC 1 cut(s) 309
NdeII GATC 9 cut(s) 75, 135, 536, 906, 1001, 1138, 1154, 1516, 1608
NheI GCTAGC 1 cut(s) 989
NlaIII CATG 2 cut(s) 575, 1425
NlaIV GGNNCC 4 cut(s) 310, 471, 538, 1390
NmeAIII GCCGAG 3 cut(s) 378, 402, 1639
NmuCI GTSAC 3 cut(s) 641, 1145, 1492
NsbI TGCGCA 1 cut(s) 37
NspI RCATGY 1 cut(s) 575
NspV TTCGAA 1 cut(s) 888
PaeR7I CTCGAG 1 cut(s) 1187
PaqCI CACCTGC 1 cut(s) 289
PasI CCCWGGG 1 cut(s) 27
PceI AGGCCT 1 cut(s) 191
PciI ACATGT 1 cut(s) 571
PcsI WCGNNNNNNNCGW 4 cut(s) 279, 285, 288, 1525
PctI GAATGC 1 cut(s) 664
PdmI GAANNNNTTC 3 cut(s) 116, 884, 1486
PfeI GAWTC 3 cut(s) 776, 1094, 1126
PflFI GACNNNGTC 2 cut(s) 275, 287
Ple19I CGATCG 1 cut(s) 1519
PleI GAGTC 2 cut(s) 1373, 1549
PluTI GGCGCC 1 cut(s) 312
PpsI GAGTC 2 cut(s) 1373, 1549
PscI ACATGT 1 cut(s) 571
PshAI GACNNNNGTC 1 cut(s) 1499
Psp124BI GAGCTC 1 cut(s) 1186
Psp6I CCWGG 4 cut(s) 26, 324, 653, 1663
PspFI CCCAGC 2 cut(s) 44, 494
PspGI CCWGG 4 cut(s) 26, 324, 653, 1663
PspN4I GGNNCC 4 cut(s) 310, 471, 538, 1390
PspPI GGNCC 1 cut(s) 181
PstI CTGCAG 2 cut(s) 739, 1283
PstNI CAGNNNCTG 1 cut(s) 44
PsuI RGATCY 3 cut(s) 536, 1001, 1154
PsyI GACNNNGTC 2 cut(s) 275, 287
PvuI CGATCG 1 cut(s) 1519
RsaI GTAC 2 cut(s) 1313, 1322
RsaNI GTAC 2 cut(s) 1312, 1321
RseI CAYNNNNRTG 1 cut(s) 1016
SacI GAGCTC 1 cut(s) 1186
SalI GTCGAC 4 cut(s) 278, 290, 953, 1367
SaqAI TTAA 4 cut(s) 165, 717, 1134, 1308
Sau3AI GATC 9 cut(s) 75, 135, 536, 906, 1001, 1138, 1154, 1516, 1608
Sau96I GGNCC 1 cut(s) 181
SchI GAGTC 2 cut(s) 1374, 1549
ScrFI CCNGG 4 cut(s) 28, 326, 655, 1665
SduI GDGCHC 2 cut(s) 1186, 1699
SexAI ACCWGGT 1 cut(s) 324
SfaNI GCATC 3 cut(s) 339, 593, 1069
SfcI CTRYAG 3 cut(s) 735, 997, 1279
SfiI GGCCNNNNNGGCC 1 cut(s) 398
SfoI GGCGCC 1 cut(s) 310
Sfr274I CTCGAG 1 cut(s) 1187
SfuI TTCGAA 1 cut(s) 888
SinI GGWCC 1 cut(s) 181
SlaI CTCGAG 1 cut(s) 1187
SmiMI CAYNNNNRTG 1 cut(s) 1016
SmlI CTYRAG 2 cut(s) 1187, 1299
SmoI CTYRAG 2 cut(s) 1187, 1299
SseBI AGGCCT 1 cut(s) 191
SsiI CCGC 9 cut(s) 42, 234, 275, 339, 405, 491, 680, 1329, 1497
SspDI GGCGCC 1 cut(s) 308
SspMI CTAG 3 cut(s) 552, 990, 1685
SstI GAGCTC 1 cut(s) 1186
StuI AGGCCT 1 cut(s) 191
StyD4I CCNGG 4 cut(s) 26, 324, 653, 1663
TaaI ACNGT 4 cut(s) 841, 1151, 1373, 1466
TaiI ACGT 1 cut(s) 954
TatI WGTACW 2 cut(s) 1311, 1320
TauI GCSGC 3 cut(s) 44, 342, 494
TfiI GAWTC 3 cut(s) 776, 1094, 1126
Tru1I TTAA 4 cut(s) 165, 717, 1134, 1308
Tru9I TTAA 4 cut(s) 165, 717, 1134, 1308
TscAI CASTG 7 cut(s) 510, 567, 1081, 1154, 1183, 1288, 1497
TseFI GTSAC 3 cut(s) 641, 1145, 1492
Tsp45I GTSAC 3 cut(s) 641, 1145, 1492
TspDTI ATGAA 4 cut(s) 17, 789, 862, 1410
TspGWI ACGGA 5 cut(s) 916, 1344, 1560, 1633, 1640
TspRI CASTG 7 cut(s) 510, 567, 1081, 1154, 1183, 1288, 1497
Tth111I GACNNNGTC 2 cut(s) 275, 287
VpaK11BI GGWCC 1 cut(s) 181
XagI CCTNNNNNAGG 1 cut(s) 428
XapI RAATTY 5 cut(s) 83, 673, 890, 1208, 1382
XceI RCATGY 1 cut(s) 575
XhoI CTCGAG 1 cut(s) 1187
XmiI GTMKAC 4 cut(s) 279, 291, 954, 1368
XmnI GAANNNNTTC 3 cut(s) 116, 884, 1486
XspI CTAG 3 cut(s) 552, 990, 1685
ZraI GACGTC 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.