pycom10g16480

Histone H1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003009
Physical Location & Seq
Reverse (-)
19692411 .. 19693183
773 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGATCAAATTAATTGAGCACAAAAGCACACCCAAACCAAGAATGAACACCTCATTTTCCAAACCCAGCCATCTACACCTCTCCCACCCAGAGCATCCACAACCGTCCACAACGGTTCATCCAAAGGCCCACATTCAAATTTCCGAAAGGCAGCGATCCTCGTGCTGGTCCTGTCGACCAATCAGCGCCCAGCTTTCGGATATCGTCCCTCAGGCACTTGACTATTTAAAGAGTCCAATTTCCGTCATCTTCTCACACAACGAAATTACCTGTGAATTCACACACACGTACATTGCCGGTCGATCCAGATCTCCCTCCCCTCTTTCAAATTCCAGTCCTCTTCCCTTTCCCCTTTATTTCCAAACCCTAGCCCTAAAATTCGAATTGTTCATGGCGACCGAAGAAGCCGCCGTGGTCGTCGTGACCGAGACGATCGAGAATCCTCCTCCCGAGCCGGCCACCACCGAGACGGCGGCCGACAAACCGGTGGCCAAGACCGGCAAGGCCAAGGAGCCCAAGGCGAAGAAGGCGCCCGCTCCGAGGAAGCCCAGAAGCGCTCCAGCTCATCCTCCGTACGAAGAGATGGTTAAGGACGCGATTGTGACGTTGAAGGAGAGGACTGGTTCGAGCCAGTACGCGATCGATCTTTACTAA

Protein Analysis

218

Amino Acids

24.07

Weight (kDa)

9.07

Isoelectric Point (pI)

63.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 189 - 215 3.3e-08 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 529
AccBSI CCGCTC 1 cut(s) 536
AccI GTMKAC 1 cut(s) 175
AccII CGCG 2 cut(s) 596, 638
AciI CCGC 3 cut(s) 408, 473, 534
AclWI GGATC 2 cut(s) 150, 297
AcoI YGGCCR 3 cut(s) 456, 474, 489
AcsI RAATTY 4 cut(s) 138, 275, 328, 377
AcyI GRCGYC 1 cut(s) 530
AfaI GTAC 3 cut(s) 290, 575, 635
AfeI AGCGCT 1 cut(s) 556
AfiI CCNNNNNNNGG 3 cut(s) 165, 196, 540
AflIII ACRYGT 1 cut(s) 285
AgeI ACCGGT 1 cut(s) 484
AgsI TTSAA 3 cut(s) 137, 327, 610
AluBI AGCT 2 cut(s) 193, 563
AluI AGCT 2 cut(s) 193, 563
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 2 cut(s) 422, 461
AlwI GGATC 2 cut(s) 150, 297
Ama87I CYCGRG 1 cut(s) 449
Aor51HI AGCGCT 1 cut(s) 556
AoxI GGCC 5 cut(s) 126, 456, 474, 489, 504
ApeKI GCWGC 1 cut(s) 151
ApoI RAATTY 4 cut(s) 138, 275, 328, 377
AseI ATTAAT 1 cut(s) 11
AsiGI ACCGGT 1 cut(s) 484
AspLEI GCGC 3 cut(s) 188, 532, 557
AspS9I GGNCC 2 cut(s) 127, 168
AsuII TTCGAA 1 cut(s) 381
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 1 cut(s) 168
AxyI CCTNAGG 1 cut(s) 210
BalI TGGCCA 1 cut(s) 491
BanI GGYRCC 1 cut(s) 529
BanII GRGCYC 1 cut(s) 516
BauI CACGAG 1 cut(s) 160
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 1 cut(s) 163
BccI CCATC 2 cut(s) 78, 577
BceAI ACGGC 2 cut(s) 395, 486
BcgI CGANNNNNNTGC 2 cut(s) 144, 178
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 2 cut(s) 422, 461
BfaI CTAG 1 cut(s) 368
BfoI RGCGCY 3 cut(s) 189, 533, 558
BglII AGATCT 1 cut(s) 308
BisI GCNGC 3 cut(s) 152, 408, 474
BlsI GCNGC 3 cut(s) 153, 409, 475
Bme18I GGWCC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 2 cut(s) 127, 168
BmiI GGNNCC 2 cut(s) 513, 531
BmsI GCATC 1 cut(s) 103
BpmI CTGGAG 1 cut(s) 543
Bpu14I TTCGAA 1 cut(s) 381
Bsa29I ATCGAT 1 cut(s) 642
BsaAI YACGTR 1 cut(s) 288
BsaBI GATNNNNATC 1 cut(s) 307
BsaHI GRCGYC 1 cut(s) 530
BsaJI CCNNGG 4 cut(s) 411, 507, 516, 539
BsaWI WCCGGW 1 cut(s) 484
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 196, 540
Bse118I RCCGGY 4 cut(s) 296, 454, 484, 497
Bse1I ACTGG 3 cut(s) 333, 625, 631
Bse21I CCTNAGG 1 cut(s) 210
Bse3DI GCAATG 1 cut(s) 291
Bse8I GATNNNNATC 1 cut(s) 307
BseCI ATCGAT 1 cut(s) 642
BseDI CCNNGG 4 cut(s) 411, 507, 516, 539
BseGI GGATG 3 cut(s) 94, 118, 565
BseJI GATNNNNATC 1 cut(s) 307
BseLI CCNNNNNNNGG 3 cut(s) 165, 196, 540
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 3 cut(s) 333, 625, 631
BseRI GAGGAG 1 cut(s) 435
BseX3I CGGCCG 1 cut(s) 474
BseXI GCAGC 1 cut(s) 163
BseYI CCCAGC 2 cut(s) 65, 189
Bsh1236I CGCG 2 cut(s) 596, 638
Bsh1285I CGRYCG 5 cut(s) 301, 399, 435, 477, 642
BshFI GGCC 5 cut(s) 128, 458, 476, 491, 506
BshNI GGYRCC 1 cut(s) 529
BshTI ACCGGT 1 cut(s) 484
BshVI ATCGAT 1 cut(s) 642
BsiEI CGRYCG 5 cut(s) 301, 399, 435, 477, 642
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 4 cut(s) 297, 455, 485, 498
BsiWI CGTACG 1 cut(s) 573
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 3 cut(s) 165, 196, 540
BsmAI GTCTC 2 cut(s) 422, 461
BsmBI CGTCTC 2 cut(s) 422, 461
BsmFI GGGAC 1 cut(s) 191
BsnI GGCC 5 cut(s) 128, 458, 476, 491, 506
BsoBI CYCGRG 1 cut(s) 449
Bsp119I TTCGAA 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 21, 516
Bsp143I GATC 7 cut(s) 3, 155, 302, 308, 432, 639, 643
BspACI CCGC 3 cut(s) 408, 473, 534
BspANI GGCC 5 cut(s) 128, 458, 476, 491, 506
BspCNI CTCAG 1 cut(s) 223
BspDI ATCGAT 1 cut(s) 642
BspFNI CGCG 2 cut(s) 596, 638
BspLI GGNNCC 2 cut(s) 513, 531
BspPI GGATC 2 cut(s) 150, 297
BspT104I TTCGAA 1 cut(s) 381
BspT107I GGYRCC 1 cut(s) 529
BsrBI CCGCTC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 4 cut(s) 296, 454, 484, 497
BsrI ACTGG 3 cut(s) 333, 625, 631
BssAI RCCGGY 4 cut(s) 296, 454, 484, 497
BssECI CCNNGG 4 cut(s) 411, 507, 516, 539
BssMI GATC 7 cut(s) 3, 155, 302, 308, 432, 639, 643
BssNI GRCGYC 1 cut(s) 530
BssSI CACGAG 1 cut(s) 160
BssT1I CCWWGG 2 cut(s) 507, 516
Bst2BI CACGAG 1 cut(s) 160
Bst4CI ACNGT 2 cut(s) 105, 115
Bst6I CTCTTC 2 cut(s) 345, 573
BstACI GRCGYC 1 cut(s) 530
BstBAI YACGTR 1 cut(s) 288
BstBI TTCGAA 1 cut(s) 381
BstC8I GCNNGC 2 cut(s) 456, 534
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 3 cut(s) 94, 118, 565
BstFNI CGCG 2 cut(s) 596, 638
BstH2I RGCGCY 3 cut(s) 189, 533, 558
BstHHI GCGC 3 cut(s) 188, 532, 557
BstKTI GATC 7 cut(s) 6, 158, 305, 311, 435, 642, 646
BstMAI GTCTC 2 cut(s) 422, 461
BstMBI GATC 7 cut(s) 3, 155, 302, 308, 432, 639, 643
BstMCI CGRYCG 5 cut(s) 301, 399, 435, 477, 642
BstMWI GCNNNNNNNGC 1 cut(s) 527
BstUI CGCG 2 cut(s) 596, 638
BstV1I GCAGC 1 cut(s) 163
BstX2I RGATCY 1 cut(s) 308
BstYI RGATCY 1 cut(s) 308
BstZI CGGCCG 1 cut(s) 474
Bsu15I ATCGAT 1 cut(s) 642
Bsu36I CCTNAGG 1 cut(s) 210
BsuRI GGCC 5 cut(s) 128, 458, 476, 491, 506
BsuTUI ATCGAT 1 cut(s) 642
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 3 cut(s) 94, 118, 565
Cac8I GCNNGC 2 cut(s) 456, 534
CfoI GCGC 3 cut(s) 188, 532, 557
Cfr10I RCCGGY 4 cut(s) 296, 454, 484, 497
Cfr13I GGNCC 2 cut(s) 127, 168
ClaI ATCGAT 1 cut(s) 642
CseI GACGC 1 cut(s) 602
Csp6I GTAC 3 cut(s) 289, 574, 634
CspAI ACCGGT 1 cut(s) 484
CviAII CATG 1 cut(s) 391
CviQI GTAC 3 cut(s) 289, 574, 634
DdeI CTNAG 1 cut(s) 210
DinI GGCGCC 1 cut(s) 531
DpnI GATC 7 cut(s) 5, 157, 304, 310, 434, 641, 645
DpnII GATC 7 cut(s) 3, 155, 302, 308, 432, 639, 643
DraI TTTAAA 1 cut(s) 228
EaeI YGGCCR 3 cut(s) 456, 474, 489
EagI CGGCCG 1 cut(s) 474
Eam1104I CTCTTC 2 cut(s) 345, 573
EarI CTCTTC 2 cut(s) 345, 573
EclXI CGGCCG 1 cut(s) 474
Eco130I CCWWGG 2 cut(s) 507, 516
Eco24I GRGCYC 1 cut(s) 516
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 1 cut(s) 168
Eco47III AGCGCT 1 cut(s) 556
Eco52I CGGCCG 1 cut(s) 474
Eco81I CCTNAGG 1 cut(s) 210
Eco88I CYCGRG 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 275
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 2 cut(s) 507, 516
EcoT38I GRGCYC 1 cut(s) 516
EgeI GGCGCC 1 cut(s) 531
EheI GGCGCC 1 cut(s) 531
ErhI CCWWGG 2 cut(s) 507, 516
Esp3I CGTCTC 2 cut(s) 422, 461
FaeI CATG 1 cut(s) 394
FaiI YATR 1 cut(s) 392
FaqI GGGAC 1 cut(s) 191
FatI CATG 1 cut(s) 390
FauI CCCGC 1 cut(s) 541
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 175
Fnu4HI GCNGC 3 cut(s) 152, 408, 474
FokI GGATG 3 cut(s) 81, 105, 552
FriOI GRGCYC 1 cut(s) 516
Fsp4HI GCNGC 3 cut(s) 152, 408, 474
FspBI CTAG 1 cut(s) 368
GlaI GCGC 3 cut(s) 187, 531, 556
GluI GCNGC 3 cut(s) 152, 408, 474
GsaI CCCAGC 2 cut(s) 69, 193
GsuI CTGGAG 1 cut(s) 543
HaeII RGCGCY 3 cut(s) 189, 533, 558
HaeIII GGCC 5 cut(s) 128, 458, 476, 491, 506
HapII CCGG 4 cut(s) 297, 455, 485, 498
HgaI GACGC 1 cut(s) 602
HhaI GCGC 3 cut(s) 188, 532, 557
Hin1I GRCGYC 1 cut(s) 530
Hin1II CATG 1 cut(s) 394
Hin6I GCGC 3 cut(s) 186, 530, 555
HinP1I GCGC 3 cut(s) 186, 530, 555
HincII GTYRAC 1 cut(s) 176
HindII GTYRAC 1 cut(s) 176
HinfI GANTC 2 cut(s) 232, 439
HpaII CCGG 4 cut(s) 297, 455, 485, 498
Hpy166II GTNNAC 2 cut(s) 108, 176
Hpy188I TCNGA 3 cut(s) 145, 199, 540
Hpy188III TCNNGA 4 cut(s) 306, 421, 436, 449
Hpy8I GTNNAC 2 cut(s) 108, 176
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 2 cut(s) 520, 604
HpyCH4III ACNGT 2 cut(s) 105, 115
HpyCH4IV ACGT 2 cut(s) 287, 605
HpyF10VI GCNNNNNNNGC 1 cut(s) 527
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 2 cut(s) 287, 605
Hsp92I GRCGYC 1 cut(s) 530
Hsp92II CATG 1 cut(s) 394
HspAI GCGC 3 cut(s) 186, 530, 555
KasI GGCGCC 1 cut(s) 529
KroI GCCGGC 1 cut(s) 454
KroNI GCCGGC 1 cut(s) 456
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 7 cut(s) 3, 155, 302, 308, 432, 639, 643
LmnI GCTCC 3 cut(s) 511, 541, 562
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 1 cut(s) 103
MaeI CTAG 1 cut(s) 368
MaeII ACGT 2 cut(s) 287, 605
MaeIII GTNAC 2 cut(s) 421, 601
MalI GATC 7 cut(s) 5, 157, 304, 310, 434, 641, 645
MbiI CCGCTC 1 cut(s) 536
MboI GATC 7 cut(s) 3, 155, 302, 308, 432, 639, 643
MboII GAAGA 5 cut(s) 241, 332, 413, 535, 590
MflI RGATCY 1 cut(s) 308
MhlI GDGCHC 2 cut(s) 21, 516
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 9 cut(s) 8, 12, 138, 237, 264, 275, 328, 377, 383
MluNI TGGCCA 1 cut(s) 491
Mly113I GGCGCC 1 cut(s) 530
MlyI GAGTC 1 cut(s) 241
Mox20I TGGCCA 1 cut(s) 491
MroNI GCCGGC 1 cut(s) 454
MscI TGGCCA 1 cut(s) 491
MseI TTAA 3 cut(s) 11, 227, 588
Msp20I TGGCCA 1 cut(s) 491
MspI CCGG 4 cut(s) 297, 455, 485, 498
MvnI CGCG 2 cut(s) 596, 638
MwoI GCNNNNNNNGC 1 cut(s) 527
NaeI GCCGGC 1 cut(s) 456
NarI GGCGCC 1 cut(s) 530
NdeII GATC 7 cut(s) 3, 155, 302, 308, 432, 639, 643
NgoMIV GCCGGC 1 cut(s) 454
NlaIII CATG 1 cut(s) 394
NlaIV GGNNCC 2 cut(s) 513, 531
NmuCI GTSAC 2 cut(s) 421, 601
NspV TTCGAA 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PdiI GCCGGC 1 cut(s) 456
PfeI GAWTC 1 cut(s) 439
Pfl23II CGTACG 1 cut(s) 573
PinAI ACCGGT 1 cut(s) 484
PkrI GCNGC 3 cut(s) 153, 409, 475
Ple19I CGATCG 2 cut(s) 435, 642
PleI GAGTC 1 cut(s) 240
PluTI GGCGCC 1 cut(s) 533
PpsI GAGTC 1 cut(s) 240
Ppu21I YACGTR 1 cut(s) 288
PshBI ATTAAT 1 cut(s) 11
PspFI CCCAGC 2 cut(s) 65, 189
PspLI CGTACG 1 cut(s) 573
PspN4I GGNNCC 2 cut(s) 513, 531
PspPI GGNCC 2 cut(s) 127, 168
PsuI RGATCY 1 cut(s) 308
PvuI CGATCG 2 cut(s) 435, 642
RsaI GTAC 3 cut(s) 290, 575, 635
RsaNI GTAC 3 cut(s) 289, 574, 634
SalI GTCGAC 1 cut(s) 174
SaqAI TTAA 3 cut(s) 11, 227, 588
SatI GCNGC 3 cut(s) 152, 408, 474
Sau3AI GATC 7 cut(s) 3, 155, 302, 308, 432, 639, 643
Sau96I GGNCC 2 cut(s) 127, 168
SchI GAGTC 1 cut(s) 241
SduI GDGCHC 2 cut(s) 21, 516
SetI ASST 7 cut(s) 53, 81, 195, 272, 290, 565, 608
SfaNI GCATC 1 cut(s) 103
SfoI GGCGCC 1 cut(s) 531
SfuI TTCGAA 1 cut(s) 381
SinI GGWCC 1 cut(s) 168
Sse9I AATT 9 cut(s) 8, 12, 138, 237, 264, 275, 328, 377, 383
SsiI CCGC 3 cut(s) 408, 473, 534
SspDI GGCGCC 1 cut(s) 529
SspMI CTAG 1 cut(s) 368
StyI CCWWGG 2 cut(s) 507, 516
TaaI ACNGT 2 cut(s) 105, 115
TaiI ACGT 2 cut(s) 290, 608
TaqI TCGA 6 cut(s) 175, 301, 381, 435, 626, 642
TaqII GACCGA 2 cut(s) 413, 440
TasI AATT 9 cut(s) 8, 12, 138, 237, 264, 275, 328, 377, 383
TauI GCSGC 2 cut(s) 410, 476
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 3 cut(s) 11, 227, 588
Tru9I TTAA 3 cut(s) 11, 227, 588
TseFI GTSAC 2 cut(s) 421, 601
TseI GCWGC 1 cut(s) 151
Tsp45I GTSAC 2 cut(s) 421, 601
TspDTI ATGAA 3 cut(s) 59, 107, 379
TspGWI ACGGA 2 cut(s) 232, 561
VpaK11BI GGWCC 1 cut(s) 168
VspI ATTAAT 1 cut(s) 11
XapI RAATTY 4 cut(s) 138, 275, 328, 377
XmiI GTMKAC 1 cut(s) 175
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.