Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007187

Physical Location & Seq

Genomic Coordinates Forward (+)

22846942 .. 22850775

Gene Structure 3834 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2991 bp

ATGGAAAGCAGCGGCGGCGACGATTGGCGAAAAGGAAGATGGAGAGACAGTGACGGCGATGAAAGAGGGAGTGGAGGAGACAATGAGACAGGAAGAGGCGGTTGGGGAAGTTATGGCGGTTCCAGGGGTGGTGGTGGTAGAGGGTCCCAGTACCACCGTCAACAACCACCGCAGGAGCAGCGTGATAACAAGTGGGGTCGTCCGGGGAGACCTTCTCCATGGAGCGGTAGGCCCAACAGAGCGTGGGTGTCCACAGCGCCCTCCCCTGCATCTCCAGCCCTAGCGAACTCTCCTACTTCCCCTCCTCCTCCTGATACTTTTGTTCCTCGATTGCGATCTCTGGAAATTTCGAAACAGACGCCTCCTTCATCTTCTTCGGACAATGCAGACAAGAATCTCTCAGCAAAACGGCCAGACAACGGAGGCACAAAAGCTATCCAAACTGTTATGCTTCGTTCTAATCATTTTAATGTCTCATTCAATCCTGAGAGTATCATAATGCACTACGGTGTTGATGTCAAATCAGAGACCCCTGCTCCTAATCATATGAAAATTTCCAAGTCTGAGCTCTCTGCAATCAGGAAGAAGTTATCCTCTGATAAACCTACGGACTTTCCCTTGTCAAGTACTGCATACGATGGTGAAAAGAACATCTTCAGTGCAGTGCGGTTGCCCACTGGATCATTTAAGGTAGAGGTTCCTGATGAAGAAGACACGCGGTGTACTACATACATATTTACTATAAAGATAGTAAACGAGCTCAAGCTTTGCAAGTTGAAGGAGTACTTGAGTGGCCACTTATTGTCTATCCCTCGTGATATATTACACGGTATGGATTTGGTGATGAAAGAGAATCCAAGTAGGCGCTTGATTGCTGTTGGGCGAAACTATTACCCTGTGGATTCTAGTCTCAACGACGACCTTGGACATGGTATTTCTGCATCTAGAGGGTTTCAAGATTCTGTAAGGCTCACTTCCCAGGGTCCTTCCCTGTGTCTTGATTACTCAGTCCTGGCATTTTATAAGCGCTTGCCAGTTATAGATTTTCTCCAAGAGCAGATTACGGGTTTTACTTTAAATAATTTCGAGAGGTTTAGGAGAGAGGTTGAGCGTGTGTTGAGAGGACTGAAAGTAACTGTGATGCACCGTAAAACCAAGCAAAAGTACATCATCAAGGGGCTGACTGATAAGAATGCAGGAGATATTACATTTTATGATGTAGACATAGACGGCAAGTTTCCACCAAGGAAAGTTAGACTTGTCGATTATTTCAGGGAAAGATACAAGGAAATCCAGTATACAGACATTCCTTGCTTGGATTTGGGGAAAAATGGTAGAAGGAATGATACACCTATGGAGTTCTGTGTGTTAGCTGAGGGGCAAAAGTATTCAAAGGAGCGCTTGGACAGAGAAGCAGCCATGATGCTGAGGAGTAGGTCATTGGCTCAACCACGTGATAGGCAGAGGAAGATATGTGACATTATACAGTCTGACGATGGACCCTGTGGTGGTGGCATAATCGAAAATTTTGGAATTGAAGTTAACAAGAATATGACACCAGTAACAGGGCGTGTCATTGGCCCACCAGAATTGAAGTTGGGTGCTTCTGATGGCAAGGTGAATAAGGTAACAGTTGACAGAGAGAAATGCAACTGGAATCTGATTGGAAAGTCTGTAATGGAAGGAAAACCAATCAATCGCTGGGCTTTACTTGACTTCACCCGTCATGATCGATTTGTACTGAACCACGGCTTCATCCCAAAGCTCATCAATCGGTGCAATGAGCTAGGAATCAGAATGGAAGAGCCTCTTTCATACCAAGGCACTACAATGAGACCATTCTCTAGTGTTCAGATGCTTCGCCAGGTACTTGAAAGTGTTAATGAAGAGGTACACAAGAAAGGAAAAGGCAACTTGCAATTAATTATTTGTGTAATGCCTAGAAGAGATCCTGGTTACAAGTATCTGAAGTGGATCTCCGAGACTCAAATTGGTATAGTGACGCAGTGCTGCTTGTCAAATATGGCCAACAAAGCAAATGAAAAGTATCTTGGAAACCTTGCACTTAAGATTAATGCAAAGCTTGGAGGTAGTAATGTAGAACTAATTGATCGACTCCCCCTCTTTGGGGATGCAGGCCATGTTATGTTTGTGGGGGCTGATGTCAATCATCCTGCTGCGCAAAACAAAAAAAGTCCATCAATTGCAGCTGTTGTTGCCACTGTAAACTGGCCTGCTTTGAATCGTTATGCTGCACGAGTTCGACCCCAGCAACACCGTAGCGAAAGGATGTTGAGTTTTGGAGACATGTGCCTGGAGCTTGTTGAAACTTATGAGCGATTGAATAAAGTCAAGCCAGATAAAATTGTTGTCTTCCGTGATGGAGTCAGCGAGGGCCAGTTTGATATGGTTCTAAATGAAGAGTTACTAGATATGAAGAGGGCTTTTGGATGTAAAAAATACTATCCTACCGTCACACTTATTGTTGCTCAAAAGCGGCACCATACTCGTTTGTTTCTAGAGAATGTGAAGGATGGGAGTCCAACATGCAATGTGTTACCGGGGACTGTTGTGGATACAACAATTGTGCACCCGTTTGAGTTTGACTTTTATCTTTGCAGTCATTATGGAGCCCTCGGAACAAGCAAACCCACACACTACTATGTTCTTTGGGATGAGCATAGGTTTACCTCTGACCAATTGCAGAAGCTCATTTATGACTTGTGCTTCACCTTAGCAAGGTGTACTAAACCTGTAGCTTTGGTACCACCGGTATACTATGCTGACCTGGTTGCATATAGAGGGCGGTTATACCATGAGAGTATCGAGCGACAGTCTCGAGATTCAGTATCTTCTTCCTCCTCCTATTCTTCATCATCATCAGCAGCAGCAGCAGCAGCATTGGCATCGTCACCACACTCAGTGGCTTCTCTAGACGGAAAGTTTTATAAATTGCATGCTGACCTGGAAAACATTATGTTTTTCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0016032 GO:0019048 GO:0035197 GO:0035821 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0061980 GO:0097159 GO:0098542 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

997

Amino Acids

111.91

Weight (kDa)

9.18

Isoelectric Point (pI)

45.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	148 - 282	1.6e-20	N-terminal domain of argonaute
ArgoL1	PF08699	293 - 342	1.1e-11	Argonaute linker 1 domain
PAZ	PF02170	361 - 470	1.5e-20	PAZ domain
ArgoL2	PF16488	505 - 532	1.6e-06	Argonaute linker 2 domain
Piwi	PF02171	641 - 936	1.8e-88	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31280 AT1G31290 AT1G31290 AT1G31290
fragaria_vesca	FvH4_3g11980
malus_domestica	MD05G1256100.v1.1 MD10G1236300.v1.1 MD10G1236400.v1.1
prunus_persica	Prupe.4G108000_v2.0.a1 Prupe.4G108100_v2.0.a1
pyrus_communis	pycom05g23500 pycom05g23510 pycom05g23520 pycom10g19770 pycom10g19780
rosa_chinensis	RchiOBHm_Chr5g0019581
rosa_laevigata	RLG00000032457
rosa_roxburghii	Rroxscaffold_1G00058500
rosa_rugosa	Rorug05G0052100 Rorug05G0052200
rosa_samantha	Rh5AG142700 Rh5BG141200 Rh5CG153300 Rh5DG141900
rosa_wichuraiana	Rw5G012630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1023, 2949
Acc16I	TGCGCA	1 cut(s)	2181
Acc65I	GGTACC	1 cut(s)	2764
AccB1I	GGYRCC	2 cut(s)	2499, 2764
AccBSI	CCGCTC	1 cut(s)	225
AccI	GTMKAC	3 cut(s)	1221, 1298, 2775
AccII	CGCG	1 cut(s)	718
AclWI	GGATC	3 cut(s)	688, 1943, 1982
AcoI	YGGCCR	3 cut(s)	410, 793, 2025
AcsI	RAATTY	3 cut(s)	345, 552, 1525
AcuI	CTGAAG	2 cut(s)	640, 1988
AcvI	CACGTG	1 cut(s)	1454
AcyI	GRCGYC	1 cut(s)	359
AdeI	CACNNNGTG	2 cut(s)	720, 2923
AfeI	AGCGCT	2 cut(s)	1028, 1400
AfiI	CCNNNNNNNGG	8 cut(s)	224, 419, 1508, 1565, 2126, 2127, 2128, 2739
AflII	CTTAAG	1 cut(s)	2066
AflIII	ACRYGT	1 cut(s)	2307
AgeI	ACCGGT	1 cut(s)	2770
AjnI	CCWGG	8 cut(s)	122, 978, 1011, 1863, 1951, 2313, 2787, 2964
AjuI	GAANNNNNNNTTGG	4 cut(s)	85, 117, 2034, 2066
AleI	CACNNNNGTG	1 cut(s)	507
Alw21I	GWGCWC	3 cut(s)	570, 762, 2592
Alw44I	GTGCAC	1 cut(s)	2588
AlwI	GGATC	3 cut(s)	688, 1943, 1982
Ama87I	CYCGRG	1 cut(s)	2838
Aor51HI	AGCGCT	2 cut(s)	1028, 1400
AoxI	GGCC	8 cut(s)	230, 410, 793, 1579, 2025, 2137, 2231, 2395
ApaLI	GTGCAC	1 cut(s)	2588
ApoI	RAATTY	3 cut(s)	345, 552, 1525
AseI	ATTAAT	2 cut(s)	1922, 2073
AsiGI	ACCGGT	1 cut(s)	2770
Asp700I	GAANNNNTTC	1 cut(s)	653
Asp718I	GGTACC	1 cut(s)	2764
AspLEI	GCGC	5 cut(s)	259, 867, 1029, 1401, 2182
AspS9I	GGNCC	6 cut(s)	144, 231, 983, 1499, 1580, 2395
AsuC2I	CCSGG	2 cut(s)	204, 2562
AsuHPI	GGTGA	6 cut(s)	653, 853, 1630, 1711, 2722, 2904
AsuII	TTCGAA	1 cut(s)	350
AvaI	CYCGRG	1 cut(s)	2838
AvaII	GGWCC	3 cut(s)	144, 983, 1499
BaeGI	GKGCMC	1 cut(s)	2592
BaeI	ACNNNNGTAYC	2 cut(s)	306, 339
BalI	TGGCCA	2 cut(s)	795, 2027
BanI	GGYRCC	2 cut(s)	2499, 2764
BanII	GRGCYC	3 cut(s)	570, 762, 2635
BarI	GAAGNNNNNNTAC	2 cut(s)	1330, 1362
BauI	CACGAG	2 cut(s)	813, 2256
BbrPI	CACGTG	1 cut(s)	1454
BbsI	GAAGAC	2 cut(s)	717, 2365
Bbv12I	GWGCWC	3 cut(s)	570, 762, 2592
BbvCI	CCTCAGC	2 cut(s)	1374, 1427
BccI	CCATC	7 cut(s)	33, 632, 1490, 1604, 2206, 2375, 2528
BceAI	ACGGC	4 cut(s)	70, 425, 1246, 1765
BcgI	CGANNNNNNTGC	4 cut(s)	2489, 2523, 2889, 2923
BciT130I	CCWGG	8 cut(s)	124, 980, 1013, 1865, 1953, 2315, 2789, 2966
BciVI	GTATCC	1 cut(s)	2569
BcnI	CCSGG	2 cut(s)	204, 2562
BfaI	CTAG	9 cut(s)	281, 906, 945, 1787, 1845, 1941, 2429, 2519, 2933
BfmI	CTRYAG	1 cut(s)	2754
BfoI	RGCGCY	4 cut(s)	260, 868, 1030, 1402
BfrI	CTTAAG	1 cut(s)	2066
BfuI	GTATCC	1 cut(s)	2569
BmcAI	AGTACT	2 cut(s)	628, 785
Bme18I	GGWCC	3 cut(s)	144, 983, 1499
BmeT110I	CYCGRG	1 cut(s)	2838
BmgT120I	GGNCC	6 cut(s)	144, 231, 983, 1499, 1580, 2395
BmiI	GGNNCC	9 cut(s)	121, 145, 146, 699, 984, 1501, 2501, 2632, 2766
BmrI	ACTGGG	1 cut(s)	142
BmsI	GCATC	7 cut(s)	278, 950, 1131, 1413, 1845, 2122, 2915
BmuI	ACTGGG	1 cut(s)	142
BpiI	GAAGAC	2 cut(s)	717, 2365
BplI	GAGNNNNNCTC	2 cut(s)	199, 231
BpmI	CTGGAG	2 cut(s)	258, 2336
Bpu10I	CCTNAGC	3 cut(s)	1374, 1427, 2734
Bpu14I	TTCGAA	1 cut(s)	350
BpuEI	CTTGAG	2 cut(s)	746, 808
BpuMI	CCSGG	2 cut(s)	204, 2562
Bsa29I	ATCGAT	1 cut(s)	1732
BsaAI	YACGTR	1 cut(s)	1454
BsaBI	GATNNNNATC	2 cut(s)	334, 2166
BsaHI	GRCGYC	1 cut(s)	359
BsaI	GGTCTC	3 cut(s)	202, 521, 1828
BsaWI	WCCGGW	1 cut(s)	2770
BsaXI	ACNNNNNCTCC	6 cut(s)	34, 64, 286, 316, 520, 550
Bsc4I	CCNNNNNNNGG	8 cut(s)	224, 419, 1508, 1565, 2126, 2127, 2128, 2739
Bse118I	RCCGGY	1 cut(s)	2770
Bse1I	ACTGG	8 cut(s)	148, 682, 1034, 1294, 1559, 1658, 2234, 2398
Bse3DI	GCAATG	2 cut(s)	1786, 2557
Bse8I	GATNNNNATC	2 cut(s)	334, 2166
BseBI	CCWGG	8 cut(s)	124, 980, 1013, 1865, 1953, 2315, 2789, 2966
BseCI	ATCGAT	1 cut(s)	1732
BseGI	GGATG	7 cut(s)	1755, 2137, 2170, 2295, 2456, 2539, 2680
BseJI	GATNNNNATC	2 cut(s)	334, 2166
BseLI	CCNNNNNNNGG	8 cut(s)	224, 419, 1508, 1565, 2126, 2127, 2128, 2739
BseMI	GCAATG	2 cut(s)	1786, 2557
BseMII	CTCAG	7 cut(s)	414, 477, 555, 1020, 1365, 1418, 2934
BseNI	ACTGG	8 cut(s)	148, 682, 1034, 1294, 1559, 1658, 2234, 2398
BseRI	GAGGAG	5 cut(s)	90, 294, 297, 1444, 2851
BseSI	GKGCMC	1 cut(s)	2592
BseYI	CCCAGC	2 cut(s)	1701, 2268
BsgI	GTGCAG	2 cut(s)	681, 2238
Bsh1236I	CGCG	1 cut(s)	718
BshFI	GGCC	8 cut(s)	232, 412, 795, 1581, 2027, 2139, 2233, 2397
BshNI	GGYRCC	2 cut(s)	2499, 2764
BshTI	ACCGGT	1 cut(s)	2770
BshVI	ATCGAT	1 cut(s)	1732
BsiHKAI	GWGCWC	3 cut(s)	570, 762, 2592
BsiHKCI	CYCGRG	1 cut(s)	2838
BsiSI	CCGG	3 cut(s)	203, 2561, 2771
BslFI	GGGAC	2 cut(s)	130, 2578
BslI	CCNNNNNNNGG	8 cut(s)	224, 419, 1508, 1565, 2126, 2127, 2128, 2739
BsmFI	GGGAC	2 cut(s)	130, 2578
BsmI	GAATGC	1 cut(s)	1198
BsnI	GGCC	8 cut(s)	232, 412, 795, 1581, 2027, 2139, 2233, 2397
Bso31I	GGTCTC	3 cut(s)	202, 521, 1828
BsoBI	CYCGRG	1 cut(s)	2838
Bsp119I	TTCGAA	1 cut(s)	350
Bsp1286I	GDGCHC	4 cut(s)	570, 762, 2592, 2635
Bsp143I	GATC	6 cut(s)	335, 680, 1729, 1948, 1974, 2110
Bsp19I	CCATGG	1 cut(s)	218
BspANI	GGCC	8 cut(s)	232, 412, 795, 1581, 2027, 2139, 2233, 2397
BspCNI	CTCAG	7 cut(s)	413, 478, 556, 1019, 1366, 1419, 2933
BspDI	ATCGAT	1 cut(s)	1732
BspFNI	CGCG	1 cut(s)	718
BspHI	TCATGA	1 cut(s)	1726
BspLI	GGNNCC	9 cut(s)	121, 145, 146, 699, 984, 1501, 2501, 2632, 2766
BspPI	GGATC	3 cut(s)	688, 1943, 1982
BspQI	GCTCTTC	1 cut(s)	1797
BspT104I	TTCGAA	1 cut(s)	350
BspT107I	GGYRCC	2 cut(s)	2499, 2764
BspTI	CTTAAG	1 cut(s)	2066
BspTNI	GGTCTC	3 cut(s)	202, 521, 1828
BsrBI	CCGCTC	1 cut(s)	225
BsrDI	GCAATG	2 cut(s)	1786, 2557
BsrFI	RCCGGY	1 cut(s)	2770
BsrI	ACTGG	8 cut(s)	148, 682, 1034, 1294, 1559, 1658, 2234, 2398
BssAI	RCCGGY	1 cut(s)	2770
BssMI	GATC	6 cut(s)	335, 680, 1729, 1948, 1974, 2110
BssNAI	GTATAC	2 cut(s)	1299, 2776
BssNI	GRCGYC	1 cut(s)	359
BssSI	CACGAG	2 cut(s)	813, 2256
BssT1I	CCWWGG	4 cut(s)	218, 922, 1244, 1819
Bst1107I	GTATAC	2 cut(s)	1299, 2776
Bst2BI	CACGAG	2 cut(s)	813, 2256
Bst2UI	CCWGG	8 cut(s)	124, 980, 1013, 1865, 1953, 2315, 2789, 2966
Bst6I	CTCTTC	6 cut(s)	88, 1797, 1881, 1939, 2415, 2432
BstACI	GRCGYC	1 cut(s)	359
BstAFI	CTTAAG	1 cut(s)	2066
BstBAI	YACGTR	1 cut(s)	1454
BstBI	TTCGAA	1 cut(s)	350
BstC8I	GCNNGC	4 cut(s)	1031, 2137, 2235, 2958
BstDEI	CTNAG	8 cut(s)	400, 486, 564, 1006, 1374, 1427, 2734, 2920
BstDSI	CCRYGG	2 cut(s)	218, 1747
BstENI	CCTNNNNNAGG	1 cut(s)	2737
BstF5I	GGATG	7 cut(s)	1755, 2137, 2170, 2295, 2456, 2539, 2680
BstFNI	CGCG	1 cut(s)	718
BstH2I	RGCGCY	4 cut(s)	260, 868, 1030, 1402
BstHHI	GCGC	5 cut(s)	259, 867, 1029, 1401, 2182
BstKTI	GATC	6 cut(s)	338, 683, 1732, 1951, 1977, 2113
BstMBI	GATC	6 cut(s)	335, 680, 1729, 1948, 1974, 2110
BstMWI	GCNNNNNNNGC	9 cut(s)	15, 178, 275, 2033, 2216, 2891, 2894, 2897, 2903
BstNI	CCWGG	8 cut(s)	124, 980, 1013, 1865, 1953, 2315, 2789, 2966
BstNSI	RCATGY	3 cut(s)	2311, 2550, 2960
BstSFI	CTRYAG	1 cut(s)	2754
BstSLI	GKGCMC	1 cut(s)	2592
BstUI	CGCG	1 cut(s)	718
BstV2I	GAAGAC	2 cut(s)	717, 2365
BstX2I	RGATCY	2 cut(s)	1948, 1974
BstYI	RGATCY	2 cut(s)	1948, 1974
BstZ17I	GTATAC	2 cut(s)	1299, 2776
Bsu15I	ATCGAT	1 cut(s)	1732
BsuI	GTATCC	1 cut(s)	2569
BsuRI	GGCC	8 cut(s)	232, 412, 795, 1581, 2027, 2139, 2233, 2397
BsuTUI	ATCGAT	1 cut(s)	1732
BtgI	CCRYGG	2 cut(s)	218, 1747
BtgZI	GCGATG	1 cut(s)	72
BtsCI	GGATG	7 cut(s)	1755, 2137, 2170, 2295, 2456, 2539, 2680
BtsI	GCAGTG	2 cut(s)	669, 2012
BtsIMutI	CAGTG	7 cut(s)	55, 664, 669, 675, 2012, 2220, 2928
Cac8I	GCNNGC	4 cut(s)	1031, 2137, 2235, 2958
CciI	TCATGA	1 cut(s)	1726
CfoI	GCGC	5 cut(s)	259, 867, 1029, 1401, 2182
Cfr10I	RCCGGY	1 cut(s)	2770
Cfr13I	GGNCC	6 cut(s)	144, 231, 983, 1499, 1580, 2395
ClaI	ATCGAT	1 cut(s)	1732
CseI	GACGC	2 cut(s)	367, 2011
CsiI	ACCWGGT	1 cut(s)	2787
CspAI	ACCGGT	1 cut(s)	2770
CspCI	CAANNNNNGTGG	2 cut(s)	1572, 1607
CviAII	CATG	9 cut(s)	219, 929, 1420, 1727, 2141, 2308, 2547, 2816, 2957
DdeI	CTNAG	8 cut(s)	400, 486, 564, 1006, 1374, 1427, 2734, 2920
DpnI	GATC	6 cut(s)	337, 682, 1731, 1950, 1976, 2112
DpnII	GATC	6 cut(s)	335, 680, 1729, 1948, 1974, 2110
DraI	TTTAAA	1 cut(s)	1077
DraIII	CACNNNGTG	2 cut(s)	720, 2923
EaeI	YGGCCR	3 cut(s)	410, 793, 2025
Eam1104I	CTCTTC	6 cut(s)	88, 1797, 1881, 1939, 2415, 2432
EarI	CTCTTC	6 cut(s)	88, 1797, 1881, 1939, 2415, 2432
Ecl136II	GAGCTC	2 cut(s)	568, 760
Eco130I	CCWWGG	4 cut(s)	218, 922, 1244, 1819
Eco24I	GRGCYC	3 cut(s)	570, 762, 2635
Eco31I	GGTCTC	3 cut(s)	202, 521, 1828
Eco47I	GGWCC	3 cut(s)	144, 983, 1499
Eco47III	AGCGCT	2 cut(s)	1028, 1400
Eco53kI	GAGCTC	2 cut(s)	568, 760
Eco57I	CTGAAG	2 cut(s)	640, 1988
Eco72I	CACGTG	1 cut(s)	1454
Eco88I	CYCGRG	1 cut(s)	2838
EcoICRI	GAGCTC	2 cut(s)	568, 760
EcoNI	CCTNNNNNAGG	1 cut(s)	2737
EcoO109I	RGGNCCY	2 cut(s)	144, 983
EcoRII	CCWGG	8 cut(s)	122, 978, 1011, 1863, 1951, 2313, 2787, 2964
EcoT14I	CCWWGG	4 cut(s)	218, 922, 1244, 1819
EcoT38I	GRGCYC	3 cut(s)	570, 762, 2635
ErhI	CCWWGG	4 cut(s)	218, 922, 1244, 1819
FaeI	CATG	9 cut(s)	222, 932, 1423, 1730, 2144, 2311, 2550, 2819, 2960
FaqI	GGGAC	2 cut(s)	130, 2578
FatI	CATG	9 cut(s)	218, 928, 1419, 1726, 2140, 2307, 2546, 2815, 2956
FauNDI	CATATG	2 cut(s)	546, 2987
FblI	GTMKAC	3 cut(s)	1221, 1298, 2775
FokI	GGATG	7 cut(s)	1742, 2144, 2157, 2302, 2463, 2546, 2687
FriOI	GRGCYC	3 cut(s)	570, 762, 2635
FspBI	CTAG	9 cut(s)	281, 906, 945, 1787, 1845, 1941, 2429, 2519, 2933
FspI	TGCGCA	1 cut(s)	2181
GlaI	GCGC	5 cut(s)	258, 866, 1028, 1400, 2181
GsaI	CCCAGC	2 cut(s)	1705, 2272
GsuI	CTGGAG	2 cut(s)	258, 2336
HaeII	RGCGCY	4 cut(s)	260, 868, 1030, 1402
HaeIII	GGCC	8 cut(s)	232, 412, 795, 1581, 2027, 2139, 2233, 2397
HapII	CCGG	3 cut(s)	203, 2561, 2771
HgaI	GACGC	2 cut(s)	367, 2011
HhaI	GCGC	5 cut(s)	259, 867, 1029, 1401, 2182
Hin1I	GRCGYC	1 cut(s)	359
Hin1II	CATG	9 cut(s)	222, 932, 1423, 1730, 2144, 2311, 2550, 2819, 2960
Hin6I	GCGC	5 cut(s)	257, 865, 1027, 1399, 2180
HinP1I	GCGC	5 cut(s)	257, 865, 1027, 1399, 2180
HincII	GTYRAC	3 cut(s)	161, 1543, 1636
HindII	GTYRAC	3 cut(s)	161, 1543, 1636
HindIII	AAGCTT	2 cut(s)	764, 2081
HpaI	GTTAAC	1 cut(s)	1543
HpaII	CCGG	3 cut(s)	203, 2561, 2771
HphI	GGTGA	6 cut(s)	653, 853, 1630, 1711, 2722, 2904
Hpy99I	CGWCG	2 cut(s)	23, 920
HpyAV	CCTTC	7 cut(s)	222, 375, 772, 996, 1332, 1676, 2524
HpyCH4IV	ACGT	1 cut(s)	1453
HpyF10VI	GCNNNNNNNGC	9 cut(s)	15, 178, 275, 2033, 2216, 2891, 2894, 2897, 2903
HpyF3I	CTNAG	8 cut(s)	400, 486, 564, 1006, 1374, 1427, 2734, 2920
HpySE526I	ACGT	1 cut(s)	1453
Hsp92I	GRCGYC	1 cut(s)	359
Hsp92II	CATG	9 cut(s)	222, 932, 1423, 1730, 2144, 2311, 2550, 2819, 2960
HspAI	GCGC	5 cut(s)	257, 865, 1027, 1399, 2180
KflI	GGGWCCC	1 cut(s)	144
KpnI	GGTACC	1 cut(s)	2768
KspAI	GTTAAC	1 cut(s)	1543
Kzo9I	GATC	6 cut(s)	335, 680, 1729, 1948, 1974, 2110
LguI	GCTCTTC	1 cut(s)	1797
LmnI	GCTCC	6 cut(s)	175, 222, 541, 1396, 2317, 2630
LweI	GCATC	7 cut(s)	278, 950, 1131, 1413, 1845, 2122, 2915
MabI	ACCWGGT	1 cut(s)	2787
MaeI	CTAG	9 cut(s)	281, 906, 945, 1787, 1845, 1941, 2429, 2519, 2933
MaeII	ACGT	1 cut(s)	1453
MalI	GATC	6 cut(s)	337, 682, 1731, 1950, 1976, 2112
MbiI	CCGCTC	1 cut(s)	225
MboI	GATC	6 cut(s)	335, 680, 1729, 1948, 1974, 2110
MfeI	CAATTG	3 cut(s)	2202, 2583, 2699
MflI	RGATCY	2 cut(s)	1948, 1974
MhlI	GDGCHC	4 cut(s)	570, 762, 2592, 2635
MlsI	TGGCCA	2 cut(s)	795, 2027
MluNI	TGGCCA	2 cut(s)	795, 2027
MlyI	GAGTC	4 cut(s)	1978, 2109, 2394, 2548
MmeI	TCCRAC	1 cut(s)	2567
Mox20I	TGGCCA	2 cut(s)	795, 2027
MroXI	GAANNNNTTC	1 cut(s)	653
MscI	TGGCCA	2 cut(s)	795, 2027
MseI	TTAA	8 cut(s)	468, 687, 1076, 1542, 1881, 1922, 2067, 2073
MslI	CAYNNNNRTG	4 cut(s)	468, 507, 1829, 2661
Msp20I	TGGCCA	2 cut(s)	795, 2027
MspA1I	CMGCKG	2 cut(s)	12, 2210
MspCI	CTTAAG	1 cut(s)	2066
MspI	CCGG	3 cut(s)	203, 2561, 2771
MunI	CAATTG	3 cut(s)	2202, 2583, 2699
Mva1269I	GAATGC	1 cut(s)	1198
MvaI	CCWGG	8 cut(s)	124, 980, 1013, 1865, 1953, 2315, 2789, 2966
MvnI	CGCG	1 cut(s)	718
MwoI	GCNNNNNNNGC	9 cut(s)	15, 178, 275, 2033, 2216, 2891, 2894, 2897, 2903
NciI	CCSGG	2 cut(s)	204, 2562
NcoI	CCATGG	1 cut(s)	218
NdeI	CATATG	2 cut(s)	546, 2987
NdeII	GATC	6 cut(s)	335, 680, 1729, 1948, 1974, 2110
NlaIII	CATG	9 cut(s)	222, 932, 1423, 1730, 2144, 2311, 2550, 2819, 2960
NlaIV	GGNNCC	9 cut(s)	121, 145, 146, 699, 984, 1501, 2501, 2632, 2766
NmuCI	GTSAC	5 cut(s)	50, 1475, 1999, 2473, 2910
NsbI	TGCGCA	1 cut(s)	2181
NspI	RCATGY	3 cut(s)	2311, 2550, 2960
NspV	TTCGAA	1 cut(s)	350
OliI	CACNNNNGTG	1 cut(s)	507
PaeI	GCATGC	1 cut(s)	2960
PaeR7I	CTCGAG	1 cut(s)	2838
PagI	TCATGA	1 cut(s)	1726
PasI	CCCWGGG	1 cut(s)	979
PciI	ACATGT	1 cut(s)	2307
PciSI	GCTCTTC	1 cut(s)	1797
PctI	GAATGC	1 cut(s)	1198
PdmI	GAANNNNTTC	1 cut(s)	653
PfeI	GAWTC	8 cut(s)	394, 853, 902, 959, 1657, 1791, 2242, 2843
PinAI	ACCGGT	1 cut(s)	2770
PleI	GAGTC	4 cut(s)	1978, 2109, 2393, 2547
PmaCI	CACGTG	1 cut(s)	1454
PmlI	CACGTG	1 cut(s)	1454
PpsI	GAGTC	4 cut(s)	1978, 2109, 2393, 2547
Ppu21I	YACGTR	1 cut(s)	1454
PpuMI	RGGWCCY	2 cut(s)	144, 983
PscI	ACATGT	1 cut(s)	2307
PshBI	ATTAAT	2 cut(s)	1922, 2073
PsiI	TTATAA	2 cut(s)	1023, 2949
Psp124BI	GAGCTC	2 cut(s)	570, 762
Psp5II	RGGWCCY	2 cut(s)	144, 983
Psp6I	CCWGG	8 cut(s)	122, 978, 1011, 1863, 1951, 2313, 2787, 2964
PspCI	CACGTG	1 cut(s)	1454
PspFI	CCCAGC	2 cut(s)	1701, 2268
PspGI	CCWGG	8 cut(s)	122, 978, 1011, 1863, 1951, 2313, 2787, 2964
PspN4I	GGNNCC	9 cut(s)	121, 145, 146, 699, 984, 1501, 2501, 2632, 2766
PspPI	GGNCC	6 cut(s)	144, 231, 983, 1499, 1580, 2395
PspPPI	RGGWCCY	2 cut(s)	144, 983
PsuI	RGATCY	2 cut(s)	1948, 1974
PvuII	CAGCTG	1 cut(s)	2210
RseI	CAYNNNNRTG	4 cut(s)	468, 507, 1829, 2661
SacI	GAGCTC	2 cut(s)	570, 762
SapI	GCTCTTC	1 cut(s)	1797
SaqAI	TTAA	8 cut(s)	468, 687, 1076, 1542, 1881, 1922, 2067, 2073
Sau3AI	GATC	6 cut(s)	335, 680, 1729, 1948, 1974, 2110
Sau96I	GGNCC	6 cut(s)	144, 231, 983, 1499, 1580, 2395
ScaI	AGTACT	2 cut(s)	628, 785
SchI	GAGTC	4 cut(s)	1978, 2109, 2394, 2548
SduI	GDGCHC	4 cut(s)	570, 762, 2592, 2635
SexAI	ACCWGGT	1 cut(s)	2787
SfaNI	GCATC	7 cut(s)	278, 950, 1131, 1413, 1845, 2122, 2915
SfcI	CTRYAG	1 cut(s)	2754
Sfr274I	CTCGAG	1 cut(s)	2838
SfuI	TTCGAA	1 cut(s)	350
SinI	GGWCC	3 cut(s)	144, 983, 1499
SlaI	CTCGAG	1 cut(s)	2838
SmiMI	CAYNNNNRTG	4 cut(s)	468, 507, 1829, 2661
SmlI	CTYRAG	4 cut(s)	761, 787, 2066, 2838
SmoI	CTYRAG	4 cut(s)	761, 787, 2066, 2838
SphI	GCATGC	1 cut(s)	2960
SspMI	CTAG	9 cut(s)	281, 906, 945, 1787, 1845, 1941, 2429, 2519, 2933
SstI	GAGCTC	2 cut(s)	570, 762
StyI	CCWWGG	4 cut(s)	218, 922, 1244, 1819
TaiI	ACGT	1 cut(s)	1456
TatI	WGTACW	6 cut(s)	626, 722, 783, 1164, 1738, 2744
TauI	GCSGC	3 cut(s)	15, 18, 2500
TfiI	GAWTC	8 cut(s)	394, 853, 902, 959, 1657, 1791, 2242, 2843
Tru1I	TTAA	8 cut(s)	468, 687, 1076, 1542, 1881, 1922, 2067, 2073
Tru9I	TTAA	8 cut(s)	468, 687, 1076, 1542, 1881, 1922, 2067, 2073
TscAI	CASTG	7 cut(s)	55, 664, 669, 682, 2012, 2227, 2928
TseFI	GTSAC	5 cut(s)	50, 1475, 1999, 2473, 2910
Tsp45I	GTSAC	5 cut(s)	50, 1475, 1999, 2473, 2910
TspGWI	ACGGA	4 cut(s)	435, 623, 2366, 2952
TspRI	CASTG	7 cut(s)	55, 664, 669, 682, 2012, 2227, 2928
Vha464I	CTTAAG	1 cut(s)	2066
VneI	GTGCAC	1 cut(s)	2588
VpaK11BI	GGWCC	3 cut(s)	144, 983, 1499
VspI	ATTAAT	2 cut(s)	1922, 2073
XagI	CCTNNNNNAGG	1 cut(s)	2737
XapI	RAATTY	3 cut(s)	345, 552, 1525
XbaI	TCTAGA	3 cut(s)	944, 2518, 2932
XceI	RCATGY	3 cut(s)	2311, 2550, 2960
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1698
XhoI	CTCGAG	1 cut(s)	2838
XmiI	GTMKAC	3 cut(s)	1221, 1298, 2775
XmnI	GAANNNNTTC	1 cut(s)	653
XspI	CTAG	9 cut(s)	281, 906, 945, 1787, 1845, 1941, 2429, 2519, 2933
ZrmI	AGTACT	2 cut(s)	628, 785

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.