pycom10g20940

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002321
Physical Location & Seq
Forward (+)
23691748 .. 23700016
8269 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2328 bp
ATGTTTTGGCGTATGGCGGGGTTGTCCACGGCGTCTGCGGTGGAGACAATTTTGGACAAGGATCATTTCACGTTGGAAGCGCTACTTGATGAAGATGAAATTATTCAAGAATGCAAAGCTCTTAATGGCCGCCTAATCAATTTTTTGCGAGAAAGAGCCCAGGTTGAGCAACTCATCCGATACATTGTTGAAGAAGCTCCCGAGGATGCTGAAAATGGACGAACTTTCAAATTTCCTTTTATTGCATGTGAGATTTTTACTTGTGAAGTTGATATCATACTCAAAACATTGGTAGAGGATGAGGAGTTGATGAACTTGTTGTTCTCCTTTTTGGAACCAAATAATTCTCACAGCACACTCTTGGCTGGGTACTTTAGCAAGGTTGTTGTTTGTCTGCTGTTGCGCAAGGCAGTTCCTTTCCTGCATTATATTCAGGGTCATCAAGATATTGTCAAGAAGCTAGTTGACCTGATTGGAATTACATCCATTATGGAGGTTTTGATACGTCTGGTTGGTGCTGATGAACACATGTATTCTAACTATACGGATGCAATGCACTGGATACAAGATACAGATGTGCTAGAGATGATTGTGGACAAGTTCAGCTCTTCGGATTCTCCTGAAGTGCATGCTAATGCAGCGGAGACACTTTGTGCTATAACACGGTTTGCTCCGCCTGGACTTTCTTCTAAAATCTCAAGCCCAAGCTTTATAGGAAGATTGTTTCGGCATGCTTTGGAGGATTCACGGCCAAAATCTGTTCTTGTTAACTCATTGTCGGTATGCATCTCTTTGTTAGACCCTAGGAGGCTAACCTTGGGAACGTATCATATGTATGGACGTCAGATGACTCATGGATCCACAGTTACCGCTAATCCTGAGACTGTTGTGGGCATGCTGGAGAGCCTAGGTGATTTGCTGAAGCTGTTGGATGTTTCGTCGGTGGATAATACGCTGTTAACTACATATGGCAAGTTACAGCCGCCTCTTGGAAAACATCGATTAAAGGTTGTGGAGTTCATTTCAGTATTACTTACGGTTGGTAGTGAAGCTGCTGAGAAGGAATTGATTCGTCTTGGAGCTGTACAAATAATTTTAGACTTGTTTTTTAAGTACCCATATAACAACTTTGTACATCACCACATAGAGAACATAATTGTGTCGTGTTTGGAAAGCAAGAATGTTCCTCTTGTTGAACATCTTCTTCGTGAGTGTGACCTTGTTGGAAAAGTACTTCAAGCAGAAAAGAATTTTACATTGGTAGCTGACTCGGACAAGCCAACTGTTCCTGCAGAGGGTAGATCCCCACCCAGAATTGGGAGCATTGGACATTTAACACGTATATCCAACAAACTCATTCAGCTAGGAAATAACAATAGCGAGATTCAGACGCTTCTGCAGGAAAACAGTGAGTGGGCTGAGTGGCAGGCAAGTGTCCTGTCAAAACGTAACGCAGTCGAGAACGTCTATCAATGGGCCTGTGGGCGGCCAACTGCATTACATGATCGAAATAGGGATAGTGATGATGATGACTATCAAGATAGAGATTACGATGTTGCAGCCCTTGCAAATAACTTAAGCCAGGCTTTCCGATATGGAATTAACAACAATGATGTGTTAGATGAGGGCCATGGCTCACTTGAACGAGATGATGAGGAAGTTTACTTTGATGATGAATCTGCTGAAGTCATCATATCTTCATTGCGTTTGGGAGATGACCAGGAAAGTGGTTCTCTGTTTACAAATTCCAACTGGTTTGCTTTCGAGGATGACAGGGCTGCCACTGATCACTCAACTGGCTCTCTTGCTTCTCCGTCACCTATTACGGCGGAGTCTAATGTTGTCAATGGTGGCGGGGAAGATGATGTCATTGTTGGTGAAGATGATGACTTAGTTGACACCGCAACATCTTCTCCGGAACCAGGAATAAAACCAGAAGATTCGGAAGTTGCCATCCCAAACAATTCGTTAGAATTGGGGCCCGTTGAAACTGAAAAACCACCTGAGTGGGTTGAATGGAGGGAGACTTCAGACTCTGGGGAACCCTCTGATGCCCTATCAAATGGTGAGCTTCAAACGAAATCTGCGGATGCAGCTAAATGTTCGTCATCTTCTGAGCCATTGATGAAAGATGATAAAGTTGTTACCGAGCCTCAAGCTGAATCAACAGTTGAGGGAACTTCCAAGCCGGCTGAGCTGTCAGAAACAGGCAATGAGAAGACAAGTTCTGAGTCAACCACTTCTGATGCTGCAGCTGCCGACGTGGGAACAAAAGGCAGTCAAGAAGCAGCTCCGGGTGATAAAGAGAAAGATAAGGAAGGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

776

Amino Acids

85.4

Weight (kDa)

4.52

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAPS PF04499 131 - 489 1e-61 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 844
Acc16I TGCGCA 1 cut(s) 404
AccIII TCCGGA 1 cut(s) 1915
AclWI GGATC 4 cut(s) 69, 852, 865, 1296
AcoI YGGCCR 3 cut(s) 127, 749, 1487
AcsI RAATTY 3 cut(s) 230, 1249, 1744
AcuI CTGAAG 4 cut(s) 642, 941, 1704, 2013
AcyI GRCGYC 2 cut(s) 32, 841
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 5 cut(s) 371, 1086, 1115, 1134, 1233
AfeI AGCGCT 1 cut(s) 81
AfiI CCNNNNNNNGG 6 cut(s) 989, 1295, 1316, 1317, 1485, 1922
AflII CTTAAG 1 cut(s) 1576
AflIII ACRYGT 2 cut(s) 528, 1337
AgsI TTSAA 9 cut(s) 107, 191, 229, 1196, 1238, 1643, 1988, 2015, 2075
AjiI CACGTC 1 cut(s) 2263
AjnI CCWGG 5 cut(s) 159, 676, 1581, 1719, 1921
AjuI GAANNNNNNNTTGG 2 cut(s) 1241, 1273
AleI CACNNNNGTG 1 cut(s) 2005
Alw26I GTCTC 4 cut(s) 38, 638, 875, 2018
AlwI GGATC 4 cut(s) 69, 852, 865, 1296
AlwNI CAGNNNCTG 1 cut(s) 2036
Ama87I CYCGRG 1 cut(s) 200
Aor13HI TCCGGA 1 cut(s) 1915
Aor51HI AGCGCT 1 cut(s) 81
AoxI GGCC 6 cut(s) 127, 749, 1476, 1487, 1627, 1979
ApaI GGGCCC 1 cut(s) 1983
ApeKI GCWGC 9 cut(s) 638, 1052, 1559, 1778, 2093, 2249, 2252, 2255, 2288
ApoI RAATTY 3 cut(s) 230, 1249, 1744
Asp700I GAANNNNTTC 3 cut(s) 102, 1068, 1200
AspA2I CCTAGG 2 cut(s) 803, 907
AspLEI GCGC 2 cut(s) 82, 405
AspS9I GGNCC 4 cut(s) 1476, 1627, 1979, 1980
AsuC2I CCSGG 1 cut(s) 2295
AsuHPI GGTGA 6 cut(s) 923, 1130, 1809, 1889, 2078, 2309
AvaI CYCGRG 1 cut(s) 200
AvrII CCTAGG 2 cut(s) 803, 907
BaeGI GKGCMC 1 cut(s) 1983
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BamHI GGATCC 1 cut(s) 857
BanII GRGCYC 2 cut(s) 160, 1983
BbsI GAAGAC 1 cut(s) 2225
BbvI GCAGC 9 cut(s) 650, 1039, 1571, 1765, 2105, 2236, 2242, 2264, 2300
BccI CCATC 1 cut(s) 1961
BceAI ACGGC 3 cut(s) 45, 764, 1842
BciT130I CCWGG 5 cut(s) 161, 678, 1583, 1721, 1923
BciVI GTATCC 1 cut(s) 555
BclI TGATCA 1 cut(s) 1786
BcnI CCSGG 1 cut(s) 2295
BcoDI GTCTC 4 cut(s) 38, 638, 875, 2018
BfaI CTAG 6 cut(s) 461, 581, 804, 908, 1364, 2326
BfmI CTRYAG 3 cut(s) 1290, 1397, 2250
BfoI RGCGCY 1 cut(s) 83
BfrI CTTAAG 1 cut(s) 1576
BfuI GTATCC 1 cut(s) 555
BlnI CCTAGG 2 cut(s) 803, 907
BlpI GCTNAGC 1 cut(s) 2193
BmcAI AGTACT 1 cut(s) 1233
Bme1390I CCNGG 6 cut(s) 161, 678, 1583, 1721, 1923, 2295
BmeT110I CYCGRG 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 2263
BmgT120I GGNCC 4 cut(s) 1476, 1627, 1979, 1980
BmiI GGNNCC 6 cut(s) 336, 859, 1920, 1980, 1981, 2043
BmrFI CCNGG 6 cut(s) 161, 678, 1583, 1721, 1923, 2295
BmsI GCATC 6 cut(s) 196, 538, 795, 2041, 2080, 2236
BpiI GAAGAC 1 cut(s) 2225
BpmI CTGGAG 1 cut(s) 920
Bpu1102I GCTNAGC 1 cut(s) 2193
BpuEI CTTGAG 2 cut(s) 682, 2139
BpuMI CCSGG 1 cut(s) 2295
Bsa29I ATCGAT 1 cut(s) 1000
BsaAI YACGTR 1 cut(s) 1340
BsaBI GATNNNNATC 1 cut(s) 1533
BsaHI GRCGYC 2 cut(s) 32, 841
BsaJI CCNNGG 7 cut(s) 27, 159, 201, 803, 816, 907, 1630
BsaWI WCCGGW 1 cut(s) 1915
BsaXI ACNNNNNCTCC 2 cut(s) 1897, 1927
Bsc4I CCNNNNNNNGG 6 cut(s) 989, 1295, 1316, 1317, 1485, 1922
Bse118I RCCGGY 1 cut(s) 2188
Bse1I ACTGG 3 cut(s) 563, 1757, 1801
Bse3DI GCAATG 3 cut(s) 558, 1700, 2218
Bse8I GATNNNNATC 1 cut(s) 1533
BseAI TCCGGA 1 cut(s) 1915
BseBI CCWGG 5 cut(s) 161, 678, 1583, 1721, 1923
BseCI ATCGAT 1 cut(s) 1000
BseDI CCNNGG 7 cut(s) 27, 159, 201, 803, 816, 907, 1630
BseGI GGATG 9 cut(s) 174, 211, 304, 482, 553, 937, 1774, 1953, 2095
BseJI GATNNNNATC 1 cut(s) 1533
BseLI CCNNNNNNNGG 6 cut(s) 989, 1295, 1316, 1317, 1485, 1922
BseMI GCAATG 3 cut(s) 558, 1700, 2218
BseMII CTCAG 7 cut(s) 870, 1047, 1410, 1995, 2106, 2184, 2220
BseNI ACTGG 3 cut(s) 563, 1757, 1801
BseRI GAGGAG 1 cut(s) 317
BseSI GKGCMC 1 cut(s) 1983
BseXI GCAGC 9 cut(s) 650, 1039, 1571, 1765, 2105, 2236, 2242, 2264, 2300
BseYI CCCAGC 1 cut(s) 365
BshFI GGCC 6 cut(s) 129, 751, 1478, 1489, 1629, 1981
BshVI ATCGAT 1 cut(s) 1000
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 3 cut(s) 1916, 2189, 2294
BslI CCNNNNNNNGG 6 cut(s) 989, 1295, 1316, 1317, 1485, 1922
BsmAI GTCTC 4 cut(s) 38, 638, 875, 2018
BsmI GAATGC 1 cut(s) 116
BsnI GGCC 6 cut(s) 129, 751, 1478, 1489, 1629, 1981
BsoBI CYCGRG 1 cut(s) 200
Bsp120I GGGCCC 1 cut(s) 1979
Bsp1286I GDGCHC 2 cut(s) 160, 1983
Bsp13I TCCGGA 1 cut(s) 1915
Bsp1407I TGTACA 2 cut(s) 1084, 1132
Bsp143I GATC 5 cut(s) 61, 857, 1301, 1504, 1786
Bsp1720I GCTNAGC 1 cut(s) 2193
Bsp19I CCATGG 1 cut(s) 1630
BspANI GGCC 6 cut(s) 129, 751, 1478, 1489, 1629, 1981
BspCNI CTCAG 7 cut(s) 871, 1048, 1411, 1996, 2107, 2185, 2221
BspDI ATCGAT 1 cut(s) 1000
BspEI TCCGGA 1 cut(s) 1915
BspLI GGNNCC 6 cut(s) 336, 859, 1920, 1980, 1981, 2043
BspMAI CTGCAG 3 cut(s) 1294, 1401, 2254
BspPI GGATC 4 cut(s) 69, 852, 865, 1296
BspQI GCTCTTC 1 cut(s) 613
BspTI CTTAAG 1 cut(s) 1576
BsrDI GCAATG 3 cut(s) 558, 1700, 2218
BsrFI RCCGGY 1 cut(s) 2188
BsrGI TGTACA 2 cut(s) 1084, 1132
BsrI ACTGG 3 cut(s) 563, 1757, 1801
BssAI RCCGGY 1 cut(s) 2188
BssECI CCNNGG 7 cut(s) 27, 159, 201, 803, 816, 907, 1630
BssMI GATC 5 cut(s) 61, 857, 1301, 1504, 1786
BssNI GRCGYC 2 cut(s) 32, 841
BssT1I CCWWGG 4 cut(s) 803, 816, 907, 1630
Bst2UI CCWGG 5 cut(s) 161, 678, 1583, 1721, 1923
Bst4CI ACNGT 7 cut(s) 666, 865, 886, 1039, 1285, 1409, 2170
Bst6I CTCTTC 1 cut(s) 613
BstACI GRCGYC 2 cut(s) 32, 841
BstAFI CTTAAG 1 cut(s) 1576
BstAPI GCANNNNNTGC 1 cut(s) 1565
BstAUI TGTACA 2 cut(s) 1084, 1132
BstBAI YACGTR 1 cut(s) 1340
BstC8I GCNNGC 5 cut(s) 630, 732, 896, 1428, 2190
BstDEI CTNAG 8 cut(s) 879, 1056, 1419, 1891, 2004, 2115, 2193, 2229
BstDSI CCRYGG 2 cut(s) 27, 1630
BstF5I GGATG 9 cut(s) 174, 211, 304, 482, 553, 937, 1774, 1953, 2095
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 2 cut(s) 82, 405
BstKTI GATC 5 cut(s) 64, 860, 1304, 1507, 1789
BstMAI GTCTC 4 cut(s) 38, 638, 875, 2018
BstMBI GATC 5 cut(s) 61, 857, 1301, 1504, 1786
BstMWI GCNNNNNNNGC 5 cut(s) 638, 1565, 2093, 2194, 2255
BstNI CCWGG 5 cut(s) 161, 678, 1583, 1721, 1923
BstNSI RCATGY 5 cut(s) 249, 532, 632, 734, 898
BstSCI CCNGG 6 cut(s) 159, 676, 1581, 1719, 1921, 2293
BstSFI CTRYAG 3 cut(s) 1290, 1397, 2250
BstSLI GKGCMC 1 cut(s) 1983
BstV1I GCAGC 9 cut(s) 650, 1039, 1571, 1765, 2105, 2236, 2242, 2264, 2300
BstV2I GAAGAC 1 cut(s) 2225
BstX2I RGATCY 2 cut(s) 857, 1301
BstXI CCANNNNNNTGG 2 cut(s) 1727, 2007
BstYI RGATCY 2 cut(s) 857, 1301
Bsu15I ATCGAT 1 cut(s) 1000
BsuI GTATCC 1 cut(s) 555
BsuRI GGCC 6 cut(s) 129, 751, 1478, 1489, 1629, 1981
BsuTUI ATCGAT 1 cut(s) 1000
BtgI CCRYGG 2 cut(s) 27, 1630
BtrI CACGTC 1 cut(s) 2263
BtsCI GGATG 9 cut(s) 174, 211, 304, 482, 553, 937, 1774, 1953, 2095
BtsIMutI CAGTG 3 cut(s) 556, 1414, 1782
Cac8I GCNNGC 5 cut(s) 630, 732, 896, 1428, 2190
CaiI CAGNNNCTG 1 cut(s) 2036
CfoI GCGC 2 cut(s) 82, 405
Cfr10I RCCGGY 1 cut(s) 2188
Cfr13I GGNCC 4 cut(s) 1476, 1627, 1979, 1980
ClaI ATCGAT 1 cut(s) 1000
CseI GACGC 2 cut(s) 21, 1399
Csp6I GTAC 5 cut(s) 370, 1085, 1114, 1133, 1232
CviAII CATG 8 cut(s) 246, 529, 629, 731, 854, 895, 1502, 1631
CviQI GTAC 5 cut(s) 370, 1085, 1114, 1133, 1232
DdeI CTNAG 8 cut(s) 879, 1056, 1419, 1891, 2004, 2115, 2193, 2229
DpnI GATC 5 cut(s) 63, 859, 1303, 1506, 1788
DpnII GATC 5 cut(s) 61, 857, 1301, 1504, 1786
DraIII CACNNNGTG 1 cut(s) 653
EaeI YGGCCR 3 cut(s) 127, 749, 1487
Eam1104I CTCTTC 1 cut(s) 613
EarI CTCTTC 1 cut(s) 613
EciI GGCGGA 2 cut(s) 663, 1844
Eco130I CCWWGG 4 cut(s) 803, 816, 907, 1630
Eco24I GRGCYC 2 cut(s) 160, 1983
Eco32I GATATC 1 cut(s) 274
Eco47III AGCGCT 1 cut(s) 81
Eco57I CTGAAG 4 cut(s) 642, 941, 1704, 2013
Eco88I CYCGRG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 1979
EcoRII CCWGG 5 cut(s) 159, 676, 1581, 1719, 1921
EcoRV GATATC 1 cut(s) 274
EcoT14I CCWWGG 4 cut(s) 803, 816, 907, 1630
EcoT22I ATGCAT 1 cut(s) 788
EcoT38I GRGCYC 2 cut(s) 160, 1983
ErhI CCWWGG 4 cut(s) 803, 816, 907, 1630
FaeI CATG 8 cut(s) 249, 532, 632, 734, 857, 898, 1505, 1634
FalI AAGNNNNNCTT 4 cut(s) 69, 101, 1570, 1602
FatI CATG 8 cut(s) 245, 528, 628, 730, 853, 894, 1501, 1630
FauI CCCGC 2 cut(s) 10, 1847
FauNDI CATATG 2 cut(s) 831, 967
FbaI TGATCA 1 cut(s) 1786
FokI GGATG 9 cut(s) 161, 218, 311, 469, 560, 944, 1781, 1940, 2102
FriOI GRGCYC 2 cut(s) 160, 1983
FspBI CTAG 6 cut(s) 461, 581, 804, 908, 1364, 2326
FspI TGCGCA 1 cut(s) 404
GlaI GCGC 2 cut(s) 81, 404
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 920
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 6 cut(s) 129, 751, 1478, 1489, 1629, 1981
HapII CCGG 3 cut(s) 1916, 2189, 2294
HgaI GACGC 2 cut(s) 21, 1399
HhaI GCGC 2 cut(s) 82, 405
Hin1I GRCGYC 2 cut(s) 32, 841
Hin1II CATG 8 cut(s) 249, 532, 632, 734, 857, 898, 1505, 1634
Hin6I GCGC 2 cut(s) 80, 403
HinP1I GCGC 2 cut(s) 80, 403
HincII GTYRAC 5 cut(s) 466, 769, 960, 1897, 2235
HindII GTYRAC 5 cut(s) 466, 769, 960, 1897, 2235
HindIII AAGCTT 1 cut(s) 706
HpaI GTTAAC 2 cut(s) 769, 960
HpaII CCGG 3 cut(s) 1916, 2189, 2294
HphI GGTGA 6 cut(s) 923, 1130, 1809, 1889, 2078, 2309
Hpy166II GTNNAC 9 cut(s) 27, 466, 595, 769, 960, 1663, 1740, 1897, 2235
Hpy8I GTNNAC 9 cut(s) 27, 466, 595, 769, 960, 1663, 1740, 1897, 2235
Hpy99I CGWCG 2 cut(s) 943, 2264
HpyAV CCTTC 2 cut(s) 1054, 2312
HpyCH4III ACNGT 7 cut(s) 666, 865, 886, 1039, 1285, 1409, 2170
HpyCH4IV ACGT 8 cut(s) 71, 505, 824, 841, 1339, 1447, 1464, 2262
HpyF10VI GCNNNNNNNGC 5 cut(s) 638, 1565, 2093, 2194, 2255
HpyF3I CTNAG 8 cut(s) 879, 1056, 1419, 1891, 2004, 2115, 2193, 2229
HpySE526I ACGT 8 cut(s) 71, 505, 824, 841, 1339, 1447, 1464, 2262
Hsp92I GRCGYC 2 cut(s) 32, 841
Hsp92II CATG 8 cut(s) 249, 532, 632, 734, 857, 898, 1505, 1634
HspAI GCGC 2 cut(s) 80, 403
Kpn2I TCCGGA 1 cut(s) 1915
KroI GCCGGC 1 cut(s) 2188
KroNI GCCGGC 1 cut(s) 2190
Ksp22I TGATCA 1 cut(s) 1786
KspAI GTTAAC 2 cut(s) 769, 960
Kzo9I GATC 5 cut(s) 61, 857, 1301, 1504, 1786
LguI GCTCTTC 1 cut(s) 613
LmnI GCTCC 5 cut(s) 202, 676, 1079, 1320, 2296
Lsp1109I GCAGC 9 cut(s) 650, 1039, 1571, 1765, 2105, 2236, 2242, 2264, 2300
LweI GCATC 6 cut(s) 196, 538, 795, 2041, 2080, 2236
MaeI CTAG 6 cut(s) 461, 581, 804, 908, 1364, 2326
MaeII ACGT 8 cut(s) 71, 505, 824, 841, 1339, 1447, 1464, 2262
MaeIII GTNAC 6 cut(s) 865, 975, 1214, 1448, 1815, 2143
MalI GATC 5 cut(s) 63, 859, 1303, 1506, 1788
MboI GATC 5 cut(s) 61, 857, 1301, 1504, 1786
MflI RGATCY 2 cut(s) 857, 1301
MhlI GDGCHC 2 cut(s) 160, 1983
MlyI GAGTC 5 cut(s) 844, 1262, 1841, 2027, 2240
MmeI TCCRAC 5 cut(s) 54, 909, 1204, 1371, 1773
Mph1103I ATGCAT 1 cut(s) 788
MroI TCCGGA 1 cut(s) 1915
MroNI GCCGGC 1 cut(s) 2188
MroXI GAANNNNTTC 3 cut(s) 102, 1068, 1200
MseI TTAA 8 cut(s) 123, 768, 959, 1004, 1110, 1334, 1577, 1602
MslI CAYNNNNRTG 4 cut(s) 633, 834, 1157, 2005
MspA1I CMGCKG 2 cut(s) 641, 2255
MspCI CTTAAG 1 cut(s) 1576
MspI CCGG 3 cut(s) 1916, 2189, 2294
MspR9I CCNGG 6 cut(s) 161, 678, 1583, 1721, 1923, 2295
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 5 cut(s) 161, 678, 1583, 1721, 1923
MwoI GCNNNNNNNGC 5 cut(s) 638, 1565, 2093, 2194, 2255
NaeI GCCGGC 1 cut(s) 2190
NciI CCSGG 1 cut(s) 2295
NcoI CCATGG 1 cut(s) 1630
NdeI CATATG 2 cut(s) 831, 967
NdeII GATC 5 cut(s) 61, 857, 1301, 1504, 1786
NgoMIV GCCGGC 1 cut(s) 2188
NlaIII CATG 8 cut(s) 249, 532, 632, 734, 857, 898, 1505, 1634
NlaIV GGNNCC 6 cut(s) 336, 859, 1920, 1980, 1981, 2043
NmuCI GTSAC 2 cut(s) 1214, 1815
NsbI TGCGCA 1 cut(s) 404
NsiI ATGCAT 1 cut(s) 788
NspI RCATGY 5 cut(s) 249, 532, 632, 734, 898
OliI CACNNNNGTG 1 cut(s) 2005
PaeI GCATGC 3 cut(s) 632, 734, 898
PciI ACATGT 1 cut(s) 528
PciSI GCTCTTC 1 cut(s) 613
PctI GAATGC 1 cut(s) 116
PdiI GCCGGC 1 cut(s) 2190
PdmI GAANNNNTTC 3 cut(s) 102, 1068, 1200
PfeI GAWTC 7 cut(s) 614, 743, 1069, 1384, 1676, 1940, 2162
PleI GAGTC 5 cut(s) 844, 1262, 1840, 2027, 2239
PpsI GAGTC 5 cut(s) 844, 1262, 1840, 2027, 2239
Ppu21I YACGTR 1 cut(s) 1340
PscI ACATGT 1 cut(s) 528
Psp6I CCWGG 5 cut(s) 159, 676, 1581, 1719, 1921
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 5 cut(s) 159, 676, 1581, 1719, 1921
PspN4I GGNNCC 6 cut(s) 336, 859, 1920, 1980, 1981, 2043
PspOMI GGGCCC 1 cut(s) 1979
PspPI GGNCC 4 cut(s) 1476, 1627, 1979, 1980
PstI CTGCAG 3 cut(s) 1294, 1401, 2254
PstNI CAGNNNCTG 1 cut(s) 2036
PsuI RGATCY 2 cut(s) 857, 1301
PvuII CAGCTG 1 cut(s) 2255
RsaI GTAC 5 cut(s) 371, 1086, 1115, 1134, 1233
RsaNI GTAC 5 cut(s) 370, 1085, 1114, 1133, 1232
RseI CAYNNNNRTG 4 cut(s) 633, 834, 1157, 2005
SapI GCTCTTC 1 cut(s) 613
SaqAI TTAA 8 cut(s) 123, 768, 959, 1004, 1110, 1334, 1577, 1602
Sau3AI GATC 5 cut(s) 61, 857, 1301, 1504, 1786
Sau96I GGNCC 4 cut(s) 1476, 1627, 1979, 1980
ScaI AGTACT 1 cut(s) 1233
SchI GAGTC 5 cut(s) 844, 1262, 1841, 2027, 2240
ScrFI CCNGG 6 cut(s) 161, 678, 1583, 1721, 1923, 2295
SduI GDGCHC 2 cut(s) 160, 1983
SfaNI GCATC 6 cut(s) 196, 538, 795, 2041, 2080, 2236
SfcI CTRYAG 3 cut(s) 1290, 1397, 2250
SmiMI CAYNNNNRTG 4 cut(s) 633, 834, 1157, 2005
SmlI CTYRAG 3 cut(s) 697, 1576, 2154
SmoI CTYRAG 3 cut(s) 697, 1576, 2154
SphI GCATGC 3 cut(s) 632, 734, 898
SspMI CTAG 6 cut(s) 461, 581, 804, 908, 1364, 2326
StyD4I CCNGG 6 cut(s) 159, 676, 1581, 1719, 1921, 2293
StyI CCWWGG 4 cut(s) 803, 816, 907, 1630
TaaI ACNGT 7 cut(s) 666, 865, 886, 1039, 1285, 1409, 2170
TaiI ACGT 8 cut(s) 74, 508, 827, 844, 1342, 1450, 1467, 2265
TaqI TCGA 4 cut(s) 1000, 1458, 1507, 1764
TatI WGTACW 3 cut(s) 1084, 1132, 1231
TauI GCSGC 3 cut(s) 132, 985, 1489
TfiI GAWTC 7 cut(s) 614, 743, 1069, 1384, 1676, 1940, 2162
Tru1I TTAA 8 cut(s) 123, 768, 959, 1004, 1110, 1334, 1577, 1602
Tru9I TTAA 8 cut(s) 123, 768, 959, 1004, 1110, 1334, 1577, 1602
TscAI CASTG 3 cut(s) 563, 1414, 1789
TseFI GTSAC 2 cut(s) 1214, 1815
TseI GCWGC 9 cut(s) 638, 1052, 1559, 1778, 2093, 2249, 2252, 2255, 2288
Tsp45I GTSAC 2 cut(s) 1214, 1815
TspDTI ATGAA 8 cut(s) 105, 111, 326, 537, 1009, 1689, 1689, 2141
TspGWI ACGGA 2 cut(s) 560, 1803
TspRI CASTG 3 cut(s) 563, 1414, 1789
Vha464I CTTAAG 1 cut(s) 1576
XapI RAATTY 3 cut(s) 230, 1249, 1744
XceI RCATGY 5 cut(s) 249, 532, 632, 734, 898
XmaJI CCTAGG 2 cut(s) 803, 907
XmnI GAANNNNTTC 3 cut(s) 102, 1068, 1200
XspI CTAG 6 cut(s) 461, 581, 804, 908, 1364, 2326
ZraI GACGTC 1 cut(s) 842
ZrmI AGTACT 1 cut(s) 1233
Zsp2I ATGCAT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.