pycom10g25270

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003577
Physical Location & Seq
Reverse (-)
26948096 .. 26950200
2105 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 711 bp
ATGAATTTTGTACAAAAAGCGACAGTTTTCTTTGTTTTGGCTCTTTTTGCACTGCTGTTTGTGGAGCCTGTTTCGGGTAGAAGGAAACGGTCTAGTACTGAGAAACGGATGAGGACTCAGGACCTTGTCACCAACTTGCCCGGCCAGCCTGCTGCAGACTTCAAGCACTACGCAGGCTATGTGACCGTGAACGAAACAAATGGGAGGGCTCTTTTTTACTGGTTTTATGAGGCTGCGTCCAACCCAGATGGAAAACCTCTGGTGTTGTGGCTTAATGGAGGTCCTGGGTGCTCTTCTGTGGGATATGGAGCAACCCAAGAGATTGGTCCATTCATAGTGGACACTGATGGACATGGAATTAAATCTAATAACTACTCATGGAATAAAGAGGCCAACATGTTATTCTTGGAATCTCCAGTTGGGGTTGGCTTTTCATACTCTAATACAACTAGTGATTATGATAATCTCGGAGATGGCTTTACAGCGAATGATGCGTATAACTTCTTGCATAAGTGGTATCTCAAGTTCCCATCATACAGAAGAAGGACCTTCTACATTTCTGGAGAGAGTTATGCAGGAAAGTATGTTCCGGAACTCGCTGAGCTCATATATGACAAGAACAATAATCCTTCCCTCCATATTGATCGATCAAGGGTATCCTGTTGGGTAATCCTGAAACATCTGATGCTGATGACTGGAGAGGTCTGGTAG

Protein Analysis

237

Amino Acids

26.74

Weight (kDa)

7.74

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 47 - 210 1.4e-72 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 589
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 2 cut(s) 12, 97
AfiI CCNNNNNNNGG 1 cut(s) 74
AflIII ACRYGT 1 cut(s) 396
AgsI TTSAA 1 cut(s) 163
AhlI ACTAGT 1 cut(s) 449
AjnI CCWGG 1 cut(s) 283
AjuI GAANNNNNNNTTGG 4 cut(s) 386, 402, 418, 434
AloI GAACNNNNNNTCC 2 cut(s) 570, 602
AluBI AGCT 1 cut(s) 604
AluI AGCT 1 cut(s) 604
Alw21I GWGCWC 2 cut(s) 293, 606
Aor13HI TCCGGA 1 cut(s) 589
AoxI GGCC 2 cut(s) 142, 390
ApeKI GCWGC 2 cut(s) 152, 233
ApoI RAATTY 1 cut(s) 4
AspS9I GGNCC 4 cut(s) 121, 281, 326, 546
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 1 cut(s) 121
AvaII GGWCC 4 cut(s) 121, 281, 326, 546
BanII GRGCYC 2 cut(s) 211, 606
Bbv12I GWGCWC 2 cut(s) 293, 606
BbvI GCAGC 2 cut(s) 139, 220
BccI CCATC 4 cut(s) 242, 341, 467, 538
BciT130I CCWGG 1 cut(s) 285
BciVI GTATCC 1 cut(s) 667
BcnI CCSGG 1 cut(s) 141
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 2 cut(s) 93, 450
BfmI CTRYAG 1 cut(s) 153
BfuI GTATCC 1 cut(s) 667
BisI GCNGC 2 cut(s) 153, 234
BlpI GCTNAGC 1 cut(s) 600
BlsI GCNGC 2 cut(s) 154, 235
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 2 cut(s) 141, 285
Bme18I GGWCC 4 cut(s) 121, 281, 326, 546
BmgT120I GGNCC 4 cut(s) 121, 281, 326, 546
BmiI GGNNCC 1 cut(s) 66
BmrFI CCNGG 2 cut(s) 141, 285
BmsI GCATC 2 cut(s) 481, 675
BpmI CTGGAG 2 cut(s) 399, 582
Bpu1102I GCTNAGC 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 506
BpuMI CCSGG 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 646
BsaJI CCNNGG 1 cut(s) 284
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 1 cut(s) 74
Bse1I ACTGG 3 cut(s) 224, 416, 700
BseAI TCCGGA 1 cut(s) 589
BseBI CCWGG 1 cut(s) 285
BseCI ATCGAT 1 cut(s) 646
BseDI CCNNGG 1 cut(s) 284
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 74
BseMII CTCAG 3 cut(s) 90, 131, 591
BseNI ACTGG 3 cut(s) 224, 416, 700
BseXI GCAGC 2 cut(s) 139, 220
BshFI GGCC 2 cut(s) 144, 392
BshVI ATCGAT 1 cut(s) 646
BsiHKAI GWGCWC 2 cut(s) 293, 606
BsiSI CCGG 2 cut(s) 141, 590
BslI CCNNNNNNNGG 1 cut(s) 74
BsnI GGCC 2 cut(s) 144, 392
Bsp1286I GDGCHC 3 cut(s) 211, 293, 606
Bsp13I TCCGGA 1 cut(s) 589
Bsp1407I TGTACA 1 cut(s) 10
Bsp143I GATC 2 cut(s) 643, 647
Bsp1720I GCTNAGC 1 cut(s) 600
BspANI GGCC 2 cut(s) 144, 392
BspCNI CTCAG 3 cut(s) 91, 130, 592
BspDI ATCGAT 1 cut(s) 646
BspEI TCCGGA 1 cut(s) 589
BspLI GGNNCC 1 cut(s) 66
BspMAI CTGCAG 1 cut(s) 157
BspQI GCTCTTC 1 cut(s) 298
BsrGI TGTACA 1 cut(s) 10
BsrI ACTGG 3 cut(s) 224, 416, 700
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 2 cut(s) 643, 647
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 3 cut(s) 25, 90, 187
Bst6I CTCTTC 1 cut(s) 298
BstAUI TGTACA 1 cut(s) 10
BstC8I GCNNGC 3 cut(s) 146, 150, 175
BstDEI CTNAG 3 cut(s) 99, 117, 600
BstF5I GGATG 1 cut(s) 114
BstKTI GATC 2 cut(s) 646, 650
BstMBI GATC 2 cut(s) 643, 647
BstMWI GCNNNNNNNGC 3 cut(s) 47, 145, 491
BstNI CCWGG 1 cut(s) 285
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 2 cut(s) 139, 283
BstSFI CTRYAG 1 cut(s) 153
BstV1I GCAGC 2 cut(s) 139, 220
BstXI CCANNNNNNTGG 1 cut(s) 323
Bsu15I ATCGAT 1 cut(s) 646
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 2 cut(s) 144, 392
BsuTUI ATCGAT 1 cut(s) 646
BtsCI GGATG 1 cut(s) 114
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 2 cut(s) 50, 342
Cac8I GCNNGC 3 cut(s) 146, 150, 175
Cfr13I GGNCC 4 cut(s) 121, 281, 326, 546
ClaI ATCGAT 1 cut(s) 646
CseI GACGC 1 cut(s) 225
Csp6I GTAC 2 cut(s) 11, 96
CviAII CATG 3 cut(s) 353, 378, 397
CviQI GTAC 2 cut(s) 11, 96
DdeI CTNAG 3 cut(s) 99, 117, 600
DpnI GATC 2 cut(s) 645, 649
DpnII GATC 2 cut(s) 643, 647
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
Ecl136II GAGCTC 1 cut(s) 604
Eco24I GRGCYC 2 cut(s) 211, 606
Eco47I GGWCC 4 cut(s) 121, 281, 326, 546
Eco53kI GAGCTC 1 cut(s) 604
EcoICRI GAGCTC 1 cut(s) 604
EcoO109I RGGNCCY 3 cut(s) 121, 281, 546
EcoRII CCWGG 1 cut(s) 283
EcoT38I GRGCYC 2 cut(s) 211, 606
FaeI CATG 3 cut(s) 356, 381, 400
FatI CATG 3 cut(s) 352, 377, 396
Fnu4HI GCNGC 2 cut(s) 153, 234
FokI GGATG 1 cut(s) 121
FriOI GRGCYC 2 cut(s) 211, 606
Fsp4HI GCNGC 2 cut(s) 153, 234
FspBI CTAG 2 cut(s) 93, 450
GluI GCNGC 2 cut(s) 153, 234
GsuI CTGGAG 2 cut(s) 399, 582
HaeIII GGCC 2 cut(s) 144, 392
HapII CCGG 2 cut(s) 141, 590
HgaI GACGC 1 cut(s) 225
Hin1II CATG 3 cut(s) 356, 381, 400
HinfI GANTC 2 cut(s) 115, 410
HpaII CCGG 2 cut(s) 141, 590
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 2 cut(s) 190, 340
Hpy188I TCNGA 2 cut(s) 470, 684
Hpy188III TCNNGA 4 cut(s) 119, 561, 590, 673
Hpy8I GTNNAC 2 cut(s) 190, 340
HpyAV CCTTC 4 cut(s) 75, 537, 559, 639
HpyCH4III ACNGT 3 cut(s) 25, 90, 187
HpyCH4V TGCA 4 cut(s) 50, 155, 508, 575
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 145, 491
HpyF3I CTNAG 3 cut(s) 99, 117, 600
Hsp92II CATG 3 cut(s) 356, 381, 400
Kpn2I TCCGGA 1 cut(s) 589
Kzo9I GATC 2 cut(s) 643, 647
LguI GCTCTTC 1 cut(s) 298
LmnI GCTCC 2 cut(s) 64, 308
Lsp1109I GCAGC 2 cut(s) 139, 220
LweI GCATC 2 cut(s) 481, 675
MaeI CTAG 2 cut(s) 93, 450
MaeIII GTNAC 2 cut(s) 127, 181
MalI GATC 2 cut(s) 645, 649
MboI GATC 2 cut(s) 643, 647
MboII GAAGA 2 cut(s) 285, 552
MhlI GDGCHC 3 cut(s) 211, 293, 606
MluCI AATT 2 cut(s) 4, 357
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 8 cut(s) 105, 198, 223, 267, 272, 382, 644, 694
MroI TCCGGA 1 cut(s) 589
MseI TTAA 2 cut(s) 273, 360
MspI CCGG 2 cut(s) 141, 590
MspR9I CCNGG 2 cut(s) 141, 285
MvaI CCWGG 1 cut(s) 285
MwoI GCNNNNNNNGC 3 cut(s) 47, 145, 491
NciI CCSGG 1 cut(s) 141
NdeII GATC 2 cut(s) 643, 647
NlaIII CATG 3 cut(s) 356, 381, 400
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 2 cut(s) 127, 181
NspI RCATGY 1 cut(s) 400
PciI ACATGT 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 298
PfeI GAWTC 1 cut(s) 410
PflFI GACNNNGTC 1 cut(s) 125
PkrI GCNGC 2 cut(s) 154, 235
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PpuMI RGGWCCY 3 cut(s) 121, 281, 546
PscI ACATGT 1 cut(s) 396
Psp124BI GAGCTC 1 cut(s) 606
Psp5II RGGWCCY 3 cut(s) 121, 281, 546
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 4 cut(s) 121, 281, 326, 546
PspPPI RGGWCCY 3 cut(s) 121, 281, 546
PsrI GAACNNNNNNTAC 2 cut(s) 509, 541
PstI CTGCAG 1 cut(s) 157
PsyI GACNNNGTC 1 cut(s) 125
RsaI GTAC 2 cut(s) 12, 97
RsaNI GTAC 2 cut(s) 11, 96
SacI GAGCTC 1 cut(s) 606
SapI GCTCTTC 1 cut(s) 298
SaqAI TTAA 2 cut(s) 273, 360
SatI GCNGC 2 cut(s) 153, 234
Sau3AI GATC 2 cut(s) 643, 647
Sau96I GGNCC 4 cut(s) 121, 281, 326, 546
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 141, 285
SduI GDGCHC 3 cut(s) 211, 293, 606
SetI ASST 6 cut(s) 126, 259, 283, 551, 606, 705
SfaNI GCATC 2 cut(s) 481, 675
SfcI CTRYAG 1 cut(s) 153
SinI GGWCC 4 cut(s) 121, 281, 326, 546
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
SpeI ACTAGT 1 cut(s) 449
Sse9I AATT 2 cut(s) 4, 357
SspMI CTAG 2 cut(s) 93, 450
SstI GAGCTC 1 cut(s) 606
StyD4I CCNGG 2 cut(s) 139, 283
TaaI ACNGT 3 cut(s) 25, 90, 187
TaqI TCGA 1 cut(s) 646
TasI AATT 2 cut(s) 4, 357
TatI WGTACW 2 cut(s) 10, 95
TfiI GAWTC 1 cut(s) 410
Tru1I TTAA 2 cut(s) 273, 360
Tru9I TTAA 2 cut(s) 273, 360
TscAI CASTG 2 cut(s) 57, 349
TseFI GTSAC 2 cut(s) 127, 181
TseI GCWGC 2 cut(s) 152, 233
Tsp45I GTSAC 2 cut(s) 127, 181
TspDTI ATGAA 3 cut(s) 17, 322, 423
TspGWI ACGGA 1 cut(s) 121
TspRI CASTG 2 cut(s) 57, 349
Tth111I GACNNNGTC 1 cut(s) 125
VpaK11BI GGWCC 4 cut(s) 121, 281, 326, 546
XapI RAATTY 1 cut(s) 4
XceI RCATGY 1 cut(s) 400
XspI CTAG 2 cut(s) 93, 450
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.