pycom111g00360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0005343
Physical Location & Seq
Forward (+)
262931 .. 263497
567 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00360.1

Sequence Viewer

Length: 567 bp
ATGGCTGAGACCAAGGACCAAGGCGAACTCACATTTCCCGCGAAGCGCAAACCGGATCTCAGCTGCGGTGACCAGCCCGACTGCCCCAACAAAACCCAGAAGCTTGAAGTTCCCCATCACAGTCCGTTGGCCGCCGAACCTCAAACTCAAAACCCAGAAAATTTCCACAACAATTCCTCCGCTGGCGAAGAGAAAATCTGTCAGTTTGAAGCCAGTGCTGACCTCGAAGACGAAGACGAGGAGGAAGATTACGAAGCAGAAAACGACTGTGAAAAGTCTAACGGGAAGGCGGAGGTGAATCCCAAGGGGAAGGGCATTATGAGAGATGACAAGGGCAAAGGGAAACTGGTAGAAGAAGATGACGACGACAGCGACGATGATTCGAGCGACGGCGGAAGCGAATTCGAAGACGGAGACAGTGATTTGTCGGATGATCCTCTTGCGGAGGTCGATTTGGATAACATTCTTCCTTCGAGGACTCGAAGACGGCAGGTGCAGCCAGGGGTTTACATTGCTAATGACATTGGAAACGAAGAAGAAGATGACAGTGATGACAGCGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

20.72

Weight (kDa)

4.05

Isoelectric Point (pI)

52.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 481
Acc36I ACCTGC 1 cut(s) 481
AccII CGCG 1 cut(s) 41
AciI CCGC 7 cut(s) 39, 66, 132, 180, 290, 393, 443
AclWI GGATC 2 cut(s) 63, 428
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 2 cut(s) 160, 401
AgsI TTSAA 2 cut(s) 107, 209
AjnI CCWGG 1 cut(s) 499
AluBI AGCT 2 cut(s) 63, 103
AluI AGCT 2 cut(s) 63, 103
Alw26I GTCTC 2 cut(s) 2, 408
AlwI GGATC 2 cut(s) 63, 428
AoxI GGCC 1 cut(s) 129
ApeKI GCWGC 2 cut(s) 63, 496
ApoI RAATTY 2 cut(s) 160, 401
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 2 cut(s) 80, 307
AsuII TTCGAA 1 cut(s) 405
AvaII GGWCC 1 cut(s) 16
BbsI GAAGAC 4 cut(s) 234, 240, 414, 490
BbvI GCAGC 2 cut(s) 50, 508
BccI CCATC 1 cut(s) 123
BceAI ACGGC 2 cut(s) 406, 503
BciT130I CCWGG 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 2, 408
BfuAI ACCTGC 1 cut(s) 481
BisI GCNGC 3 cut(s) 64, 132, 497
BlsI GCNGC 3 cut(s) 65, 133, 498
Bme1390I CCNGG 1 cut(s) 501
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmrFI CCNGG 1 cut(s) 501
BpiI GAAGAC 4 cut(s) 234, 240, 414, 490
Bpu14I TTCGAA 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 4 cut(s) 12, 19, 303, 500
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bse1I ACTGG 2 cut(s) 213, 351
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 1 cut(s) 501
BseDI CCNNGG 4 cut(s) 12, 19, 303, 500
BseGI GGATG 1 cut(s) 436
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 1 cut(s) 73
BseNI ACTGG 2 cut(s) 213, 351
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 2 cut(s) 50, 508
BsgI GTGCAG 1 cut(s) 515
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 1 cut(s) 131
BsiSI CCGG 1 cut(s) 53
BsmAI GTCTC 2 cut(s) 2, 408
BsnI GGCC 1 cut(s) 131
Bso31I GGTCTC 1 cut(s) 2
Bsp119I TTCGAA 1 cut(s) 405
Bsp143I GATC 2 cut(s) 55, 433
BspACI CCGC 7 cut(s) 39, 66, 132, 180, 290, 393, 443
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 1 cut(s) 72
BspFNI CGCG 1 cut(s) 41
BspMI ACCTGC 1 cut(s) 481
BspPI GGATC 2 cut(s) 63, 428
BspT104I TTCGAA 1 cut(s) 405
BspTNI GGTCTC 1 cut(s) 2
BsrDI GCAATG 1 cut(s) 510
BsrI ACTGG 2 cut(s) 213, 351
BssECI CCNNGG 4 cut(s) 12, 19, 303, 500
BssMI GATC 2 cut(s) 55, 433
BssT1I CCWWGG 3 cut(s) 12, 19, 303
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 4 cut(s) 122, 269, 419, 548
Bst6I CTCTTC 1 cut(s) 183
BstBI TTCGAA 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 2 cut(s) 6, 59
BstEII GGTNACC 1 cut(s) 68
BstF5I GGATG 1 cut(s) 436
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 2 cut(s) 58, 436
BstMAI GTCTC 2 cut(s) 2, 408
BstMBI GATC 2 cut(s) 55, 433
BstMWI GCNNNNNNNGC 1 cut(s) 496
BstNI CCWGG 1 cut(s) 501
BstPI GGTNACC 1 cut(s) 68
BstSCI CCNGG 1 cut(s) 499
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 2 cut(s) 50, 508
BstV2I GAAGAC 4 cut(s) 234, 240, 414, 490
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BsuRI GGCC 1 cut(s) 131
BtsCI GGATG 1 cut(s) 436
BtsIMutI CAGTG 3 cut(s) 220, 424, 553
BveI ACCTGC 1 cut(s) 481
Cac8I GCNNGC 1 cut(s) 184
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 16
CviJI RGCY 7 cut(s) 5, 63, 76, 103, 131, 212, 499
CviKI_1 RGCY 7 cut(s) 5, 63, 76, 103, 131, 212, 499
DdeI CTNAG 2 cut(s) 6, 59
DpnI GATC 2 cut(s) 57, 435
DpnII GATC 2 cut(s) 55, 433
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
EciI GGCGGA 2 cut(s) 305, 408
Eco130I CCWWGG 3 cut(s) 12, 19, 303
Eco31I GGTCTC 1 cut(s) 2
Eco47I GGWCC 1 cut(s) 16
Eco91I GGTNACC 1 cut(s) 68
EcoO65I GGTNACC 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 499
EcoT14I CCWWGG 3 cut(s) 12, 19, 303
ErhI CCWWGG 3 cut(s) 12, 19, 303
FaiI YATR 1 cut(s) 320
FauI CCCGC 1 cut(s) 46
Fnu4HI GCNGC 3 cut(s) 64, 132, 497
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 3 cut(s) 64, 132, 497
GlaI GCGC 1 cut(s) 47
GluI GCNGC 3 cut(s) 64, 132, 497
HaeIII GGCC 1 cut(s) 131
HapII CCGG 1 cut(s) 53
HhaI GCGC 1 cut(s) 48
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 3 cut(s) 298, 380, 478
HpaII CCGG 1 cut(s) 53
HphI GGTGA 2 cut(s) 80, 307
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 1 cut(s) 430
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 3 cut(s) 368, 377, 392
HpyAV CCTTC 3 cut(s) 280, 304, 480
HpyCH4III ACNGT 4 cut(s) 122, 269, 419, 548
HpyCH4V TGCA 1 cut(s) 496
HpyF10VI GCNNNNNNNGC 1 cut(s) 496
HpyF3I CTNAG 2 cut(s) 6, 59
HspAI GCGC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 55, 433
Lsp1109I GCAGC 2 cut(s) 50, 508
MaeIII GTNAC 1 cut(s) 68
MalI GATC 2 cut(s) 57, 435
MboI GATC 2 cut(s) 55, 433
MflI RGATCY 1 cut(s) 55
MluCI AATT 3 cut(s) 160, 172, 401
MlyI GAGTC 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 408
MnlI CCTC 9 cut(s) 150, 187, 232, 233, 235, 286, 439, 447, 468
MspA1I CMGCKG 2 cut(s) 63, 182
MspI CCGG 1 cut(s) 53
MspR9I CCNGG 1 cut(s) 501
MvaI CCWGG 1 cut(s) 501
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 496
NdeII GATC 2 cut(s) 55, 433
NmuCI GTSAC 1 cut(s) 68
NspV TTCGAA 1 cut(s) 405
PaqCI CACCTGC 1 cut(s) 481
PcsI WCGNNNNNNNCGW 3 cut(s) 369, 372, 396
PfeI GAWTC 2 cut(s) 298, 380
PkrI GCNGC 3 cut(s) 65, 133, 498
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 499
PspEI GGTNACC 1 cut(s) 68
PspGI CCWGG 1 cut(s) 499
PspPI GGNCC 1 cut(s) 16
PsuI RGATCY 1 cut(s) 55
PvuII CAGCTG 1 cut(s) 63
SatI GCNGC 3 cut(s) 64, 132, 497
Sau3AI GATC 2 cut(s) 55, 433
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 1 cut(s) 501
SetI ASST 7 cut(s) 65, 105, 142, 225, 297, 450, 495
SfuI TTCGAA 1 cut(s) 405
SinI GGWCC 1 cut(s) 16
Sse9I AATT 3 cut(s) 160, 172, 401
SsiI CCGC 7 cut(s) 39, 66, 132, 180, 290, 393, 443
StyD4I CCNGG 1 cut(s) 499
StyI CCWWGG 3 cut(s) 12, 19, 303
TaaI ACNGT 4 cut(s) 122, 269, 419, 548
TaqI TCGA 6 cut(s) 225, 383, 405, 450, 473, 481
TasI AATT 3 cut(s) 160, 172, 401
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 2 cut(s) 298, 380
TscAI CASTG 3 cut(s) 220, 424, 553
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 2 cut(s) 63, 496
Tsp45I GTSAC 1 cut(s) 68
TspGWI ACGGA 2 cut(s) 114, 426
TspRI CASTG 3 cut(s) 220, 424, 553
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 2 cut(s) 160, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.