RchiOBHm_Chr2g0176321

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005343
Physical Location & Seq
Reverse (-)
88195497 .. 88197433
1937 bp
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UTR
Exon/CDS
Intron
PRQ54334

Sequence Viewer

Length: 594 bp
ATGGCCGAAACTAAGGACCAAGAAGAAGCCACATTGCGCGCGAAGCGCAAACCCGATCCCAGCTGCGAAGACCAAGCCGACTACCCCAACAAATCCGTCAAGCTTCAAGTCTCCGGCAACAATTCTCAAGGAAAAACTCAAGAACCGCAAAATTTGAAGAGCAATTCCGAAGCTGAATTACAAGGCAATGCAGATCCCGAAGCAGAAGACGAGGAGGAATGCGGCGACGATGATGAAGAAGACGGTGAAGAGTCTAATGGGAAATCCGTGGTGGATCGCAAGGGGAAGGGGATTGTGAGAGATGATAAGGGCAAAGGGATACTGATAGAAGAAGAAGAAGAAGATGATGATGAAGATGGCGAGGAGGATAGCGACGACGACTCCTCCACCGACGACGGCGGAAGCGAATTCGAAGACGGCGACAGCGATTTATCGGACGATCCTCTTGCGGAGGTCGATTTGGACAACATTCTTCCTTCCAGGACTCGGAGACGGCAGGTTCAGCCTGGGGTTTACATCCGCAATGATAACAACGCAAACCACAACCACAACAACGATGACGATGACAGTGATGATAGCGATGGTTTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.66

Weight (kDa)

4.05

Isoelectric Point (pI)

68.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 487
AccII CGCG 2 cut(s) 39, 41
AciI CCGC 5 cut(s) 146, 222, 399, 449, 520
AclWI GGATC 4 cut(s) 50, 188, 282, 434
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 151, 407
AfiI CCNNNNNNNGG 1 cut(s) 486
AgsI TTSAA 2 cut(s) 107, 157
AjnI CCWGG 2 cut(s) 479, 505
AluBI AGCT 3 cut(s) 63, 103, 173
AluI AGCT 3 cut(s) 63, 103, 173
Alw26I GTCTC 2 cut(s) 115, 484
AlwI GGATC 4 cut(s) 50, 188, 282, 434
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 63
ApoI RAATTY 2 cut(s) 151, 407
AspLEI GCGC 3 cut(s) 39, 41, 48
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 257
AsuII TTCGAA 1 cut(s) 411
AvaII GGWCC 1 cut(s) 16
BarI GAAGNNNNNNTAC 2 cut(s) 162, 194
BbsI GAAGAC 4 cut(s) 75, 213, 246, 420
BbvI GCAGC 1 cut(s) 50
BccI CCATC 2 cut(s) 350, 575
BceAI ACGGC 3 cut(s) 412, 433, 509
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 2 cut(s) 481, 507
BciVI GTATCC 1 cut(s) 312
BcoDI GTCTC 2 cut(s) 115, 484
BfuAI ACCTGC 1 cut(s) 487
BfuI GTATCC 1 cut(s) 312
BisI GCNGC 2 cut(s) 64, 223
BlsI GCNGC 2 cut(s) 65, 224
Bme1390I CCNGG 2 cut(s) 481, 507
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmrFI CCNGG 2 cut(s) 481, 507
BpiI GAAGAC 4 cut(s) 75, 213, 246, 420
Bpu14I TTCGAA 1 cut(s) 411
BpuEI CTTGAG 2 cut(s) 111, 123
BsaJI CCNNGG 2 cut(s) 267, 506
BsaXI ACNNNNNCTCC 4 cut(s) 365, 368, 395, 398
Bsc4I CCNNNNNNNGG 1 cut(s) 486
Bse3DI GCAATG 3 cut(s) 32, 193, 529
BseBI CCWGG 2 cut(s) 481, 507
BseDI CCNNGG 2 cut(s) 267, 506
BseGI GGATG 1 cut(s) 516
BseLI CCNNNNNNNGG 1 cut(s) 486
BseMI GCAATG 3 cut(s) 32, 193, 529
BsePI GCGCGC 1 cut(s) 37
BseRI GAGGAG 3 cut(s) 227, 373, 377
BseXI GCAGC 1 cut(s) 50
BseYI CCCAGC 1 cut(s) 59
Bsh1236I CGCG 2 cut(s) 39, 41
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 486
BsmAI GTCTC 2 cut(s) 115, 484
BsmBI CGTCTC 1 cut(s) 484
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 411
Bsp143I GATC 4 cut(s) 55, 193, 274, 439
BspACI CCGC 5 cut(s) 146, 222, 399, 449, 520
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 2 cut(s) 39, 41
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 4 cut(s) 50, 188, 282, 434
BspQI GCTCTTC 1 cut(s) 152
BspT104I TTCGAA 1 cut(s) 411
BsrDI GCAATG 3 cut(s) 32, 193, 529
BssECI CCNNGG 2 cut(s) 267, 506
BssHII GCGCGC 1 cut(s) 37
BssMI GATC 4 cut(s) 55, 193, 274, 439
Bst2UI CCWGG 2 cut(s) 481, 507
Bst4CI ACNGT 2 cut(s) 245, 569
Bst6I CTCTTC 2 cut(s) 152, 243
BstBI TTCGAA 1 cut(s) 411
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 1 cut(s) 12
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 516
BstFNI CGCG 2 cut(s) 39, 41
BstHHI GCGC 3 cut(s) 39, 41, 48
BstKTI GATC 4 cut(s) 58, 196, 277, 442
BstMAI GTCTC 2 cut(s) 115, 484
BstMBI GATC 4 cut(s) 55, 193, 274, 439
BstMWI GCNNNNNNNGC 3 cut(s) 43, 45, 502
BstNI CCWGG 2 cut(s) 481, 507
BstSCI CCNGG 2 cut(s) 479, 505
BstUI CGCG 2 cut(s) 39, 41
BstV1I GCAGC 1 cut(s) 50
BstV2I GAAGAC 4 cut(s) 75, 213, 246, 420
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuI GTATCC 1 cut(s) 312
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 1 cut(s) 516
BtsIMutI CAGTG 1 cut(s) 574
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 1 cut(s) 39
CfoI GCGC 3 cut(s) 39, 41, 48
Cfr13I GGNCC 1 cut(s) 16
CviJI RGCY 7 cut(s) 5, 29, 63, 77, 103, 173, 505
CviKI_1 RGCY 7 cut(s) 5, 29, 63, 77, 103, 173, 505
DdeI CTNAG 1 cut(s) 12
DpnI GATC 4 cut(s) 57, 195, 276, 441
DpnII GATC 4 cut(s) 55, 193, 274, 439
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 152, 243
EarI CTCTTC 2 cut(s) 152, 243
EciI GGCGGA 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 2 cut(s) 479, 505
Esp3I CGTCTC 1 cut(s) 484
Fnu4HI GCNGC 2 cut(s) 64, 223
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 2 cut(s) 64, 223
GlaI GCGC 3 cut(s) 38, 40, 47
GluI GCNGC 2 cut(s) 64, 223
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 114
HhaI GCGC 3 cut(s) 39, 41, 48
Hin6I GCGC 3 cut(s) 37, 39, 46
HinP1I GCGC 3 cut(s) 37, 39, 46
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 3 cut(s) 251, 380, 484
HpaII CCGG 1 cut(s) 114
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 3 cut(s) 169, 436, 489
Hpy188III TCNNGA 2 cut(s) 140, 197
Hpy8I GTNNAC 1 cut(s) 514
Hpy99I CGWCG 5 cut(s) 230, 377, 380, 395, 398
HpyAV CCTTC 2 cut(s) 280, 486
HpyCH4III ACNGT 2 cut(s) 245, 569
HpyCH4V TGCA 1 cut(s) 191
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 45, 502
HpyF3I CTNAG 1 cut(s) 12
HspAI GCGC 3 cut(s) 37, 39, 46
Kzo9I GATC 4 cut(s) 55, 193, 274, 439
LguI GCTCTTC 1 cut(s) 152
LpnPI CCDG 7 cut(s) 73, 127, 466, 482, 492, 493, 519
Lsp1109I GCAGC 1 cut(s) 50
MalI GATC 4 cut(s) 57, 195, 276, 441
MboI GATC 4 cut(s) 55, 193, 274, 439
MflI RGATCY 1 cut(s) 193
MluCI AATT 5 cut(s) 121, 151, 163, 176, 407
MlyI GAGTC 3 cut(s) 260, 374, 478
MnlI CCTC 7 cut(s) 205, 208, 355, 358, 394, 445, 453
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 2 cut(s) 481, 507
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 2 cut(s) 481, 507
MvnI CGCG 2 cut(s) 39, 41
MwoI GCNNNNNNNGC 3 cut(s) 43, 45, 502
NdeII GATC 4 cut(s) 55, 193, 274, 439
NspV TTCGAA 1 cut(s) 411
PauI GCGCGC 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 3 cut(s) 402, 417, 423
PctI GAATGC 1 cut(s) 224
PfoI TCCNGGA 1 cut(s) 479
PkrI GCNGC 2 cut(s) 65, 224
PleI GAGTC 3 cut(s) 259, 374, 478
PpsI GAGTC 3 cut(s) 259, 374, 478
Psp6I CCWGG 2 cut(s) 479, 505
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 2 cut(s) 479, 505
PspPI GGNCC 1 cut(s) 16
PsuI RGATCY 1 cut(s) 193
PteI GCGCGC 1 cut(s) 37
PvuII CAGCTG 1 cut(s) 63
SapI GCTCTTC 1 cut(s) 152
SatI GCNGC 2 cut(s) 64, 223
Sau3AI GATC 4 cut(s) 55, 193, 274, 439
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 3 cut(s) 260, 374, 478
ScrFI CCNGG 2 cut(s) 481, 507
SetI ASST 5 cut(s) 65, 105, 175, 456, 501
SfuI TTCGAA 1 cut(s) 411
SinI GGWCC 1 cut(s) 16
SmlI CTYRAG 2 cut(s) 126, 138
SmoI CTYRAG 2 cut(s) 126, 138
Sse9I AATT 5 cut(s) 121, 151, 163, 176, 407
SsiI CCGC 5 cut(s) 146, 222, 399, 449, 520
StyD4I CCNGG 2 cut(s) 479, 505
TaaI ACNGT 2 cut(s) 245, 569
TaqI TCGA 2 cut(s) 411, 456
TasI AATT 5 cut(s) 121, 151, 163, 176, 407
TauI GCSGC 1 cut(s) 225
TscAI CASTG 1 cut(s) 574
TseI GCWGC 1 cut(s) 63
TspDTI ATGAA 2 cut(s) 249, 366
TspGWI ACGGA 2 cut(s) 85, 256
TspRI CASTG 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 2 cut(s) 151, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.