Rmu_sc0002535.1_g000017

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002535.1
OG0005343
Physical Location & Seq
Forward (+)
83496 .. 86053
2558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002535.1_g000017.1.cds

Sequence Viewer

Length: 450 bp
atgaacctgtcatacttcggctgcgaagaccaagccgactacctcaacaaatcccacaagcttcaagtctctggcaacaattctcaaggaaaaactcaagaaccagaaaatttgaagagcaattttgaagctgaattacaaggcaatgcagatcgcgaagcagaagacgaggaggacgaagaggaggaatacgacaatgaagaagaagaagaagaaggtgaattagactcatccgtggtggatcacaaggggaaggggattatgagagatgataagggcaaagagatattgatagaaaatgaagatgacgaggaggatagcgacgacggtggaagcgaattcgaagacgacgacaaaaatttgttcgacgatcctcttgcggagatcgatttggacaacattcttccttccaagactcgaagtcgacaggttcaacctgatgtaaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.95

Weight (kDa)

4.05

Isoelectric Point (pI)

68.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 424
AccII CGCG 1 cut(s) 156
AciI CCGC 1 cut(s) 380
AclWI GGATC 2 cut(s) 249, 365
AcsI RAATTY 3 cut(s) 109, 338, 358
AgsI TTSAA 4 cut(s) 65, 115, 128, 434
AhdI GACNNNNNGTC 1 cut(s) 420
AluBI AGCT 2 cut(s) 61, 131
AluI AGCT 2 cut(s) 61, 131
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 2 cut(s) 249, 365
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 3 cut(s) 109, 338, 358
AsuHPI GGTGA 1 cut(s) 230
AsuII TTCGAA 1 cut(s) 342
BarI GAAGNNNNNNTAC 2 cut(s) 120, 152
BbsI GAAGAC 3 cut(s) 33, 171, 351
BbvI GCAGC 1 cut(s) 8
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BcoDI GTCTC 1 cut(s) 73
BisI GCNGC 1 cut(s) 22
BlsI GCNGC 1 cut(s) 23
BmeRI GACNNNNNGTC 1 cut(s) 420
BpiI GAAGAC 3 cut(s) 33, 171, 351
Bpu14I TTCGAA 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 69, 81
Bsa29I ATCGAT 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 234
Bse3DI GCAATG 1 cut(s) 151
BseCI ATCGAT 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 1 cut(s) 230
BseMI GCAATG 1 cut(s) 151
BseRI GAGGAG 3 cut(s) 185, 197, 326
BseXI GCAGC 1 cut(s) 8
Bsh1236I CGCG 1 cut(s) 156
BshVI ATCGAT 1 cut(s) 387
BsmAI GTCTC 1 cut(s) 73
Bsp119I TTCGAA 1 cut(s) 342
Bsp143I GATC 4 cut(s) 151, 241, 370, 384
Bsp68I TCGCGA 1 cut(s) 156
BspACI CCGC 1 cut(s) 380
BspDI ATCGAT 1 cut(s) 387
BspFNI CGCG 1 cut(s) 156
BspPI GGATC 2 cut(s) 249, 365
BspQI GCTCTTC 1 cut(s) 110
BspT104I TTCGAA 1 cut(s) 342
BsrDI GCAATG 1 cut(s) 151
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 4 cut(s) 151, 241, 370, 384
Bst4CI ACNGT 1 cut(s) 329
Bst6I CTCTTC 2 cut(s) 110, 174
BstBI TTCGAA 1 cut(s) 342
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 1 cut(s) 230
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 4 cut(s) 154, 244, 373, 387
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 4 cut(s) 151, 241, 370, 384
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 8
BstV2I GAAGAC 3 cut(s) 33, 171, 351
Bsu15I ATCGAT 1 cut(s) 387
BsuTUI ATCGAT 1 cut(s) 387
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 1 cut(s) 230
BtuMI TCGCGA 1 cut(s) 156
ClaI ATCGAT 1 cut(s) 387
CviJI RGCY 4 cut(s) 21, 35, 61, 131
CviKI_1 RGCY 4 cut(s) 21, 35, 61, 131
DpnI GATC 4 cut(s) 153, 243, 372, 386
DpnII GATC 4 cut(s) 151, 241, 370, 384
DriI GACNNNNNGTC 1 cut(s) 420
Eam1104I CTCTTC 2 cut(s) 110, 174
Eam1105I GACNNNNNGTC 1 cut(s) 420
EarI CTCTTC 2 cut(s) 110, 174
EcoRI GAATTC 1 cut(s) 338
FaiI YATR 2 cut(s) 13, 263
FblI GTMKAC 1 cut(s) 424
Fnu4HI GCNGC 1 cut(s) 22
FokI GGATG 1 cut(s) 217
Fsp4HI GCNGC 1 cut(s) 22
GluI GCNGC 1 cut(s) 22
HincII GTYRAC 1 cut(s) 425
HindII GTYRAC 1 cut(s) 425
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 2 cut(s) 227, 415
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 2 cut(s) 425, 445
Hpy188III TCNNGA 2 cut(s) 98, 155
Hpy8I GTNNAC 2 cut(s) 425, 445
Hpy99I CGWCG 4 cut(s) 326, 329, 353, 371
HpyAV CCTTC 3 cut(s) 209, 247, 417
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4V TGCA 1 cut(s) 149
Kzo9I GATC 4 cut(s) 151, 241, 370, 384
LguI GCTCTTC 1 cut(s) 110
LpnPI CCDG 4 cut(s) 20, 57, 117, 413
Lsp1109I GCAGC 1 cut(s) 8
MalI GATC 4 cut(s) 153, 243, 372, 386
MboI GATC 4 cut(s) 151, 241, 370, 384
MluCI AATT 7 cut(s) 79, 109, 121, 134, 221, 338, 358
MlyI GAGTC 2 cut(s) 221, 409
MnlI CCTC 8 cut(s) 53, 163, 166, 175, 178, 304, 307, 384
MvnI CGCG 1 cut(s) 156
NdeII GATC 4 cut(s) 151, 241, 370, 384
NruI TCGCGA 1 cut(s) 156
NspV TTCGAA 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 110
PcsI WCGNNNNNNNCGW 3 cut(s) 174, 333, 348
PkrI GCNGC 1 cut(s) 23
PleI GAGTC 2 cut(s) 221, 409
PpsI GAGTC 2 cut(s) 221, 409
PsrI GAACNNNNNNTAC 1 cut(s) 28
RruI TCGCGA 1 cut(s) 156
SalI GTCGAC 1 cut(s) 423
SapI GCTCTTC 1 cut(s) 110
SatI GCNGC 1 cut(s) 22
Sau3AI GATC 4 cut(s) 151, 241, 370, 384
SchI GAGTC 2 cut(s) 221, 409
SetI ASST 7 cut(s) 9, 45, 63, 133, 220, 432, 439
SfuI TTCGAA 1 cut(s) 342
SmlI CTYRAG 2 cut(s) 84, 96
SmoI CTYRAG 2 cut(s) 84, 96
Sse9I AATT 7 cut(s) 79, 109, 121, 134, 221, 338, 358
SsiI CCGC 1 cut(s) 380
TaaI ACNGT 1 cut(s) 329
TaqI TCGA 5 cut(s) 342, 366, 387, 418, 424
TasI AATT 7 cut(s) 79, 109, 121, 134, 221, 338, 358
TseI GCWGC 1 cut(s) 21
TspDTI ATGAA 3 cut(s) 17, 213, 315
TspGWI ACGGA 1 cut(s) 223
XapI RAATTY 3 cut(s) 109, 338, 358
XmiI GTMKAC 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.