Rroxscaffold_2G00076570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005343
Physical Location & Seq
Forward (+)
356878 .. 359029
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076570.1

Sequence Viewer

Length: 615 bp
ATGGCCGAAACTAAGGACCAAGAAGAAGCCACATTGCACGCGAAGCGCAAACCGGATCCCAGCTGCGAAGACCAAGCCGACTACCCCAACAAATCCCCCAAGCTTCAAGTCTCCGGCAACAATTCTCAAGGAAAAACTCAAGAACCACAAAATTTGAAGAGAAATTCCGAAGCTGAATTACAAGGCAATGCAGATCCCGAAGCAGAAGTCGAGGAGTACGAAGAGGAGGAATACGGCGATGATGATGATGATGATGAAGAAGAAGAAGAAGAAGAAGACGGTCAAGAGTCTAATGGGAAATCCGTGGTGGATCGCAAGGGGAAGGGGATTATGAGAGATGATAAGGGCAAAGGGATACTGATAGAAGAAGAAGATGAAGATGGCGAGGAGGATAGCGACGACGACTCCTCCACCGACGACGGTGGAAGCGAATTCGAAGACGGCGACAGCGATTTATCGGACGATCCTCTTGCGGAGGTCGATTTGGACAACATTCTTCCTTCCAGGACTCGGAGACGGCAGGTTCAGCCTGGGGTTTACATCCGCAATGACAACAACGCAAACCACAACAACAACGAAGACGATGACAGTGATGATAGCGATGGTTTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

22.72

Weight (kDa)

4.05

Isoelectric Point (pI)

73.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 511
AccII CGCG 1 cut(s) 41
AciI CCGC 2 cut(s) 473, 544
AclWI GGATC 5 cut(s) 50, 63, 188, 318, 458
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 151, 163, 431
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 1 cut(s) 510
AgsI TTSAA 2 cut(s) 107, 157
AjnI CCWGG 2 cut(s) 503, 529
AluBI AGCT 3 cut(s) 63, 103, 173
AluI AGCT 3 cut(s) 63, 103, 173
Alw26I GTCTC 2 cut(s) 115, 508
AlwI GGATC 5 cut(s) 50, 63, 188, 318, 458
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 63
ApoI RAATTY 3 cut(s) 151, 163, 431
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 435
AvaII GGWCC 1 cut(s) 16
BamHI GGATCC 1 cut(s) 55
BarI GAAGNNNNNNTAC 2 cut(s) 162, 194
BbsI GAAGAC 4 cut(s) 75, 282, 444, 585
BbvI GCAGC 1 cut(s) 50
BccI CCATC 2 cut(s) 374, 596
BceAI ACGGC 3 cut(s) 250, 457, 533
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 2 cut(s) 505, 531
BciVI GTATCC 1 cut(s) 348
BcoDI GTCTC 2 cut(s) 115, 508
BfuAI ACCTGC 1 cut(s) 511
BfuI GTATCC 1 cut(s) 348
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme1390I CCNGG 2 cut(s) 505, 531
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 1 cut(s) 57
BmrFI CCNGG 2 cut(s) 505, 531
BpiI GAAGAC 4 cut(s) 75, 282, 444, 585
Bpu14I TTCGAA 1 cut(s) 435
BpuEI CTTGAG 2 cut(s) 111, 123
BsaJI CCNNGG 2 cut(s) 303, 530
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 4 cut(s) 389, 392, 419, 422
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse3DI GCAATG 3 cut(s) 32, 193, 553
BseBI CCWGG 2 cut(s) 505, 531
BseDI CCNNGG 2 cut(s) 303, 530
BseGI GGATG 1 cut(s) 540
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMI GCAATG 3 cut(s) 32, 193, 553
BseRI GAGGAG 4 cut(s) 227, 239, 397, 401
BseXI GCAGC 1 cut(s) 50
BseYI CCCAGC 1 cut(s) 59
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 2 cut(s) 53, 114
BslI CCNNNNNNNGG 1 cut(s) 510
BsmAI GTCTC 2 cut(s) 115, 508
BsmBI CGTCTC 1 cut(s) 508
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 435
Bsp143I GATC 4 cut(s) 55, 193, 310, 463
BspACI CCGC 2 cut(s) 473, 544
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 41
BspLI GGNNCC 1 cut(s) 57
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 5 cut(s) 50, 63, 188, 318, 458
BspT104I TTCGAA 1 cut(s) 435
BsrDI GCAATG 3 cut(s) 32, 193, 553
BssECI CCNNGG 2 cut(s) 303, 530
BssMI GATC 4 cut(s) 55, 193, 310, 463
Bst2UI CCWGG 2 cut(s) 505, 531
Bst4CI ACNGT 3 cut(s) 281, 422, 590
Bst6I CTCTTC 2 cut(s) 152, 216
BstBI TTCGAA 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 1 cut(s) 12
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 1 cut(s) 540
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 4 cut(s) 58, 196, 313, 466
BstMAI GTCTC 2 cut(s) 115, 508
BstMBI GATC 4 cut(s) 55, 193, 310, 463
BstMWI GCNNNNNNNGC 2 cut(s) 43, 526
BstNI CCWGG 2 cut(s) 505, 531
BstSCI CCNGG 2 cut(s) 503, 529
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 1 cut(s) 50
BstV2I GAAGAC 4 cut(s) 75, 282, 444, 585
BstX2I RGATCY 2 cut(s) 55, 193
BstYI RGATCY 2 cut(s) 55, 193
BsuI GTATCC 1 cut(s) 348
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 540
BtsIMutI CAGTG 1 cut(s) 595
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 1 cut(s) 39
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 217
CspCI CAANNNNNGTGG 2 cut(s) 135, 170
CviJI RGCY 7 cut(s) 5, 29, 63, 77, 103, 173, 529
CviKI_1 RGCY 7 cut(s) 5, 29, 63, 77, 103, 173, 529
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 1 cut(s) 12
DpnI GATC 4 cut(s) 57, 195, 312, 465
DpnII GATC 4 cut(s) 55, 193, 310, 463
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 152, 216
EarI CTCTTC 2 cut(s) 152, 216
Eco47I GGWCC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 503, 529
Esp3I CGTCTC 1 cut(s) 508
FaiI YATR 1 cut(s) 332
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 1 cut(s) 527
Fsp4HI GCNGC 1 cut(s) 64
GlaI GCGC 1 cut(s) 47
GluI GCNGC 1 cut(s) 64
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 53, 114
HhaI GCGC 1 cut(s) 48
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 3 cut(s) 287, 404, 508
HpaII CCGG 2 cut(s) 53, 114
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 3 cut(s) 169, 460, 513
Hpy188III TCNNGA 3 cut(s) 140, 197, 284
Hpy8I GTNNAC 1 cut(s) 538
Hpy99I CGWCG 4 cut(s) 401, 404, 419, 422
HpyAV CCTTC 2 cut(s) 316, 510
HpyCH4III ACNGT 3 cut(s) 281, 422, 590
HpyCH4V TGCA 2 cut(s) 37, 191
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 526
HpyF3I CTNAG 1 cut(s) 12
HspAI GCGC 1 cut(s) 46
Kzo9I GATC 4 cut(s) 55, 193, 310, 463
LpnPI CCDG 8 cut(s) 66, 73, 127, 490, 506, 516, 517, 543
Lsp1109I GCAGC 1 cut(s) 50
MalI GATC 4 cut(s) 57, 195, 312, 465
MboI GATC 4 cut(s) 55, 193, 310, 463
MflI RGATCY 2 cut(s) 55, 193
MluCI AATT 5 cut(s) 121, 151, 163, 176, 431
MlyI GAGTC 3 cut(s) 296, 398, 502
MnlI CCTC 8 cut(s) 205, 217, 220, 379, 382, 418, 469, 477
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 2 cut(s) 53, 114
MspR9I CCNGG 2 cut(s) 505, 531
MvaI CCWGG 2 cut(s) 505, 531
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 43, 526
NdeII GATC 4 cut(s) 55, 193, 310, 463
NlaIV GGNNCC 1 cut(s) 57
NspV TTCGAA 1 cut(s) 435
PcsI WCGNNNNNNNCGW 4 cut(s) 216, 426, 441, 447
PfoI TCCNGGA 1 cut(s) 503
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 3 cut(s) 295, 398, 502
PpsI GAGTC 3 cut(s) 295, 398, 502
Psp6I CCWGG 2 cut(s) 503, 529
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 2 cut(s) 503, 529
PspN4I GGNNCC 1 cut(s) 57
PspPI GGNCC 1 cut(s) 16
PsuI RGATCY 2 cut(s) 55, 193
PvuII CAGCTG 1 cut(s) 63
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 4 cut(s) 55, 193, 310, 463
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 3 cut(s) 296, 398, 502
ScrFI CCNGG 2 cut(s) 505, 531
SetI ASST 5 cut(s) 65, 105, 175, 480, 525
SfuI TTCGAA 1 cut(s) 435
SinI GGWCC 1 cut(s) 16
SmlI CTYRAG 2 cut(s) 126, 138
SmoI CTYRAG 2 cut(s) 126, 138
Sse9I AATT 5 cut(s) 121, 151, 163, 176, 431
SsiI CCGC 2 cut(s) 473, 544
StyD4I CCNGG 2 cut(s) 503, 529
TaaI ACNGT 3 cut(s) 281, 422, 590
TaqI TCGA 3 cut(s) 210, 435, 480
TasI AATT 5 cut(s) 121, 151, 163, 176, 431
TscAI CASTG 1 cut(s) 595
TseI GCWGC 1 cut(s) 63
TspDTI ATGAA 2 cut(s) 270, 390
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 1 cut(s) 595
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 3 cut(s) 151, 163, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.