RLG00000022403

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005343
Physical Location & Seq
Reverse (-)
83128928 .. 83129536
609 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022403

Sequence Viewer

Length: 609 bp
ATGGCCGAAACGAAGGACCAAGAAGAAGCCACATTGCACGCGAAGCGCAAACCCGATCCCAGCTGCGAAGACCAAGCCGACTACCCCAACAAATCCCACAAGCTTCAAGTCTCCGGCAACAATTCTCAAGGAAAAACTCAAGAACCACAAAATTTGAAGAGCACTTCCGAAGCTGAATTACAAGGCAATGCAGATCCCGAAGCAGAAGAGGAGGAGGACGAAGAGGAGGAATACGGCGATGATGAAGAAGAAGAAGAAGACGGTCAAGAGTCTAATGGGAAATCCGTGGTGGATCGCAAGGGGAAGGGGATTATGAGAGATGAGAAGGGCAAAGGCATACTGATAGAAGAAGAAGAAGAAGATGATGATGAAGATGGCGAGGAGGATAGCGACGAAGACTCCTCCAGCGACGACGGTGGAAGCGAATTCGAAGACGGCGACAGCGATTTATCGGACGATCCTCTTGCGGAGGTCGATTTGGACAACATTCTTCCTTCCAGGACTCGGAGACGGCAGGTTCAGCCTGGGGTTTACATCCGCAATGATAACAACGCAAACCACAACAACAACGAAGACGATGACAGTGATGATAGCGATGGTTTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

22.44

Weight (kDa)

4.05

Isoelectric Point (pI)

77.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 505
AccII CGCG 1 cut(s) 41
AciI CCGC 2 cut(s) 467, 538
AclWI GGATC 4 cut(s) 50, 188, 300, 452
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 151, 425
AfiI CCNNNNNNNGG 1 cut(s) 504
AgsI TTSAA 2 cut(s) 107, 157
AjnI CCWGG 2 cut(s) 497, 523
AluBI AGCT 3 cut(s) 63, 103, 173
AluI AGCT 3 cut(s) 63, 103, 173
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 2 cut(s) 115, 502
AlwI GGATC 4 cut(s) 50, 188, 300, 452
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 63
ApoI RAATTY 2 cut(s) 151, 425
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 429
AvaII GGWCC 1 cut(s) 16
BarI GAAGNNNNNNTAC 2 cut(s) 162, 194
BbsI GAAGAC 5 cut(s) 75, 264, 402, 438, 579
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 50
BccI CCATC 2 cut(s) 368, 590
BceAI ACGGC 3 cut(s) 250, 451, 527
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciT130I CCWGG 2 cut(s) 499, 525
BcoDI GTCTC 2 cut(s) 115, 502
BfuAI ACCTGC 1 cut(s) 505
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme1390I CCNGG 2 cut(s) 499, 525
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmrFI CCNGG 2 cut(s) 499, 525
BpiI GAAGAC 5 cut(s) 75, 264, 402, 438, 579
BpmI CTGGAG 1 cut(s) 388
Bpu14I TTCGAA 1 cut(s) 429
BpuEI CTTGAG 2 cut(s) 111, 123
BsaJI CCNNGG 2 cut(s) 285, 524
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 1 cut(s) 504
Bse3DI GCAATG 3 cut(s) 32, 193, 547
BseBI CCWGG 2 cut(s) 499, 525
BseDI CCNNGG 2 cut(s) 285, 524
BseGI GGATG 1 cut(s) 534
BseLI CCNNNNNNNGG 1 cut(s) 504
BseMI GCAATG 3 cut(s) 32, 193, 547
BseRI GAGGAG 5 cut(s) 224, 227, 239, 391, 395
BseXI GCAGC 1 cut(s) 50
BseYI CCCAGC 1 cut(s) 59
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 504
BsmAI GTCTC 2 cut(s) 115, 502
BsmBI CGTCTC 1 cut(s) 502
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 4 cut(s) 55, 193, 292, 457
BspACI CCGC 2 cut(s) 467, 538
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 41
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 4 cut(s) 50, 188, 300, 452
BspQI GCTCTTC 1 cut(s) 152
BspT104I TTCGAA 1 cut(s) 429
BsrDI GCAATG 3 cut(s) 32, 193, 547
BssECI CCNNGG 2 cut(s) 285, 524
BssMI GATC 4 cut(s) 55, 193, 292, 457
Bst2UI CCWGG 2 cut(s) 499, 525
Bst4CI ACNGT 3 cut(s) 263, 416, 584
Bst6I CTCTTC 3 cut(s) 152, 201, 216
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 39
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 534
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 4 cut(s) 58, 196, 295, 460
BstMAI GTCTC 2 cut(s) 115, 502
BstMBI GATC 4 cut(s) 55, 193, 292, 457
BstMWI GCNNNNNNNGC 2 cut(s) 43, 520
BstNI CCWGG 2 cut(s) 499, 525
BstSCI CCNGG 2 cut(s) 497, 523
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 1 cut(s) 50
BstV2I GAAGAC 5 cut(s) 75, 264, 402, 438, 579
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 285
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 534
BtsIMutI CAGTG 1 cut(s) 589
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 1 cut(s) 39
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 16
CspCI CAANNNNNGTGG 2 cut(s) 135, 170
CviJI RGCY 7 cut(s) 5, 29, 63, 77, 103, 173, 523
CviKI_1 RGCY 7 cut(s) 5, 29, 63, 77, 103, 173, 523
DpnI GATC 4 cut(s) 57, 195, 294, 459
DpnII GATC 4 cut(s) 55, 193, 292, 457
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 152, 201, 216
EarI CTCTTC 3 cut(s) 152, 201, 216
Eco47I GGWCC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 497, 523
Esp3I CGTCTC 1 cut(s) 502
FaiI YATR 2 cut(s) 314, 338
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 1 cut(s) 64
GlaI GCGC 1 cut(s) 47
GluI GCNGC 1 cut(s) 64
GsaI CCCAGC 1 cut(s) 63
GsuI CTGGAG 1 cut(s) 388
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 114
HhaI GCGC 1 cut(s) 48
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 3 cut(s) 269, 398, 502
HpaII CCGG 1 cut(s) 114
Hpy166II GTNNAC 1 cut(s) 532
Hpy188I TCNGA 3 cut(s) 169, 454, 507
Hpy188III TCNNGA 3 cut(s) 140, 197, 266
Hpy8I GTNNAC 1 cut(s) 532
Hpy99I CGWCG 3 cut(s) 395, 413, 416
HpyAV CCTTC 4 cut(s) 7, 298, 319, 504
HpyCH4III ACNGT 3 cut(s) 263, 416, 584
HpyCH4V TGCA 2 cut(s) 37, 191
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 520
HspAI GCGC 1 cut(s) 46
Kzo9I GATC 4 cut(s) 55, 193, 292, 457
LguI GCTCTTC 1 cut(s) 152
LpnPI CCDG 8 cut(s) 73, 127, 418, 484, 500, 510, 511, 537
Lsp1109I GCAGC 1 cut(s) 50
MalI GATC 4 cut(s) 57, 195, 294, 459
MboI GATC 4 cut(s) 55, 193, 292, 457
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 4 cut(s) 121, 151, 176, 425
MlyI GAGTC 3 cut(s) 278, 392, 496
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 2 cut(s) 499, 525
MvaI CCWGG 2 cut(s) 499, 525
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 43, 520
NdeII GATC 4 cut(s) 55, 193, 292, 457
NspV TTCGAA 1 cut(s) 429
PciSI GCTCTTC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 3 cut(s) 420, 435, 441
PfoI TCCNGGA 1 cut(s) 497
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 3 cut(s) 277, 392, 496
PpsI GAGTC 3 cut(s) 277, 392, 496
Psp6I CCWGG 2 cut(s) 497, 523
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 2 cut(s) 497, 523
PspPI GGNCC 1 cut(s) 16
PsuI RGATCY 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 63
SapI GCTCTTC 1 cut(s) 152
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 4 cut(s) 55, 193, 292, 457
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 3 cut(s) 278, 392, 496
ScrFI CCNGG 2 cut(s) 499, 525
SduI GDGCHC 1 cut(s) 164
SetI ASST 5 cut(s) 65, 105, 175, 474, 519
SfuI TTCGAA 1 cut(s) 429
SinI GGWCC 1 cut(s) 16
SmlI CTYRAG 2 cut(s) 126, 138
SmoI CTYRAG 2 cut(s) 126, 138
Sse9I AATT 4 cut(s) 121, 151, 176, 425
SsiI CCGC 2 cut(s) 467, 538
StyD4I CCNGG 2 cut(s) 497, 523
TaaI ACNGT 3 cut(s) 263, 416, 584
TaqI TCGA 2 cut(s) 429, 474
TasI AATT 4 cut(s) 121, 151, 176, 425
TscAI CASTG 1 cut(s) 589
TseI GCWGC 1 cut(s) 63
TspDTI ATGAA 2 cut(s) 258, 384
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 1 cut(s) 589
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 2 cut(s) 151, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.