pycom11g09880

RNase P subunit p30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002965
Physical Location & Seq
Forward (+)
8270914 .. 8274471
3558 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g09880.1

Sequence Viewer

Length: 1977 bp
ATGGCCTTCTTCGACCTCAGCATACCCTATGCGGACCCCTCCCCACCGGACCGATCATCAGCAACACGTGCCAAGCTGGTGACCAAGGCCCTGGAGCTCGGCTATTCCGGAATCGCTTACAACCGCACGATCAAGGGCGTCATGTCCGATCACGACCGTTGCACCATCCCCCTCCTCAAACTTCCTTCCCTCCTCAAGCACTCCCCTCTCCTCCACTCCTCCGTCAACTTCCACCGCGACCTCCTCGGTGTCCCCCGATCCTCCCCTTTCCGCCAGTACACCCGCCTCACCGTCTGCGCCGAGACCCCAGCCCAGTCGCAGGCCCTTAATTCCGGTAACCCGGTTCTGAAGACTTACGATTTGGTCGCCGTCAAGCCCTTGAATCAGTCGGCTTTCGACCTCGCCTGTGAGAAATTGGAGGTAGATATAATTGCGATTGATTTCTCGGAGAAGTTGCCGTTTCGATTGAAGATGCCCATGGTTAAAGCTGCAATGGAGCGCGGAGTGTATTTTGAGATCACATATGCGAATCTCATTGCCGATGTTCAAGCCAGAAAGCAAATAATAACCAATGCTAAGTTACTGGTAGATTGGACTCGTGGAAGGAATCTAATAATTTGCAGTGCTGCCCTGACTGCAAATGAATTTAGGGGGCCATATGATGTTGCAAACTTAATGTCATTACTAGGGCTCTCTATGGAGCGAGCTAAAGCGTCTATATCAAGAAATTGTAGGACTCTAGTAGCTAATGCTGTGAGAAAAAAACATTTTTTCAAGGAAACCATAAGAGTTGAAGTTCTGTCATCGGATCGAGAAACTGACAAGAACAAACCTTGGTCTGGTGACTTGTTTCAATGGATCGATCCCATCTCTAGTGGTGAAGGTGATTTGGTATTAGCTGATTTGGAAAAGTCTTTCAGTACCTCTAGTAACAAAGTATCCAAAACTGCCAAAGCCATTGATTTTGCTTCAGTCATTGACAGCATGCCGTCACTTGGGTTTCAAGTCAAGGATGTGATATGTGGCAATGATGTGGTTGTATCTCAATCAATGGCTACTTGTAAAAGCACTGTTTCTGCTTCTGAGGCAGTTGAGCAACCAGCTGTGACTGCTGGAGTGCCAGAGCAACCTGATAGAGCTTCTTTTCCTCAATCAGGTCAATCTTCTTTGTGTGATAGTCTTGTGGACCAACAAATGTTTGACTGTGAAAATCCTCAGAAATTATATTCATCTTGTGATACTGCGAACGCTTGTATTGGTGCTGTGGAAATTAAATATCCTACATTAACTTCTGAAGCAAAACCAAACAATGCAGATCGTACAGATGGGAATATTGATTTGATTTTGAACGAAATTCATGATTTGCAATCACAGAAGTGCGTGACTAATCGTGAATTGGATGTTGTGCCACCTAATGATAATTTAACATTTCAAGCGTTAGACGTTGGGTTAAATGCTGCTTGTACTGCTAATTCTGAAGTTGAAGTTTCCACAAATTACCAGGATTCGGATATTCCTGCTCCTCACAGTGAAGAGGCTTTGTGTGTGAATGGTTCTGATACACAAATTGATGTGATGGATGAAATTTTTAAAATGCTACCTGTGAAGAGTAAAGAAGAAAAATCCCTGTATTTGAATTCATCATCAACTTCAGAAAAGGAACAATTCAGGGAATCTGGAGATGATACAGTTGTACTTGCAAATCAGGTTCCTGTTCTGCAGTCTTATGATGGTATGGCGGCGCCTGCAATTGATGATTATTTAGTTGCAAGGCATGGGTCAAAGGAAGTGACTATGGAAGAGAACGAAAATGGGAAAGCTGATACTGAAATTAACTATCCAGACTCAGTTCAATCTATATCAGGGAAATCCAGACCAAAAAGGAAGACTCCTCATCAAGTGAAGTTTCTTCCTCTTAAGCGATTGTTGCATACTGTACCCCTCAAGAAGGCGCAGAAAAGTAAGAAGAAAAATTAA

Protein Analysis

659

Amino Acids

72.36

Weight (kDa)

5.74

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_P_p30 PF01876 5 - 242 4.1e-50 RNase P subunit p30
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1741
AccII CGCG 2 cut(s) 237, 501
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 7 cut(s) 32, 124, 235, 271, 283, 501, 1739
AclWI GGATC 4 cut(s) 252, 816, 857, 866
AcsI RAATTY 4 cut(s) 644, 1353, 1584, 1636
AcuI CTGAAG 5 cut(s) 368, 954, 1314, 1497, 1635
AcvI CACGTG 1 cut(s) 68
AcyI GRCGYC 2 cut(s) 138, 1742
AfaI GTAC 6 cut(s) 278, 922, 1321, 1465, 1695, 1938
AfiI CCNNNNNNNGG 6 cut(s) 319, 839, 995, 1154, 1507, 1948
AflII CTTAAG 1 cut(s) 1916
AflIII ACRYGT 1 cut(s) 65
AjnI CCWGG 2 cut(s) 90, 1500
AleI CACNNNNGTG 1 cut(s) 1375
AluBI AGCT 9 cut(s) 76, 97, 488, 707, 746, 899, 1103, 1139, 1820
AluI AGCT 9 cut(s) 76, 97, 488, 707, 746, 899, 1103, 1139, 1820
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 4 cut(s) 252, 816, 857, 866
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 4 cut(s) 3, 87, 321, 653
ApeKI GCWGC 3 cut(s) 488, 626, 1457
ApoI RAATTY 4 cut(s) 644, 1353, 1584, 1636
ArsI GACNNNNNNTTYG 4 cut(s) 343, 375, 662, 694
AspLEI GCGC 4 cut(s) 299, 501, 1744, 1954
AspS9I GGNCC 6 cut(s) 34, 49, 88, 322, 653, 1186
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 5 cut(s) 91, 280, 854, 890, 896
AvaII GGWCC 3 cut(s) 34, 49, 1186
BaeI ACNNNNGTAYC 2 cut(s) 1677, 1710
BanI GGYRCC 1 cut(s) 1741
BanII GRGCYC 2 cut(s) 99, 693
BauI CACGAG 1 cut(s) 597
BbrPI CACGTG 1 cut(s) 68
BbsI GAAGAC 2 cut(s) 356, 1892
Bbv12I GWGCWC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 17
BbvI GCAGC 3 cut(s) 475, 613, 1444
BccI CCATC 5 cut(s) 173, 875, 1319, 1570, 1724
BceAI ACGGC 3 cut(s) 353, 442, 973
BciT130I CCWGG 2 cut(s) 92, 1502
BciVI GTATCC 1 cut(s) 949
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 4 cut(s) 686, 740, 873, 927
BfmI CTRYAG 1 cut(s) 1718
BfoI RGCGCY 1 cut(s) 1745
BfrI CTTAAG 1 cut(s) 1916
BfuI GTATCC 1 cut(s) 949
BisI GCNGC 4 cut(s) 489, 627, 1458, 1740
BlsI GCNGC 4 cut(s) 490, 628, 1459, 1741
Bme1390I CCNGG 3 cut(s) 92, 341, 1502
Bme18I GGWCC 3 cut(s) 34, 49, 1186
BmgT120I GGNCC 6 cut(s) 34, 49, 88, 322, 653, 1186
BmiI GGNNCC 4 cut(s) 36, 654, 1710, 1743
BmrFI CCNGG 3 cut(s) 92, 341, 1502
BmrI ACTGGG 1 cut(s) 307
BmsI GCATC 1 cut(s) 462
BmuI ACTGGG 1 cut(s) 307
BpiI GAAGAC 2 cut(s) 356, 1892
BpmI CTGGAG 3 cut(s) 113, 1134, 1698
Bpu10I CCTNAGC 1 cut(s) 17
BpuEI CTTGAG 2 cut(s) 179, 1928
BpuMI CCSGG 1 cut(s) 341
Bsa29I ATCGAT 1 cut(s) 861
BsaAI YACGTR 1 cut(s) 68
BsaHI GRCGYC 2 cut(s) 138, 1742
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 5 cut(s) 84, 90, 244, 477, 833
BsaWI WCCGGW 3 cut(s) 46, 107, 332
Bsc4I CCNNNNNNNGG 6 cut(s) 319, 839, 995, 1154, 1507, 1948
Bse1I ACTGG 3 cut(s) 274, 313, 588
Bse3DI GCAATG 3 cut(s) 498, 534, 1033
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 2 cut(s) 92, 1502
BseCI ATCGAT 1 cut(s) 861
BseDI CCNNGG 5 cut(s) 84, 90, 244, 477, 833
BseGI GGATG 4 cut(s) 165, 1018, 1405, 1585
BseLI CCNNNNNNNGG 6 cut(s) 319, 839, 995, 1154, 1507, 1948
BseMI GCAATG 3 cut(s) 498, 534, 1033
BseMII CTCAG 4 cut(s) 31, 1074, 1229, 1860
BseNI ACTGG 3 cut(s) 274, 313, 588
BseRI GAGGAG 7 cut(s) 164, 182, 200, 208, 233, 1512, 1881
BseXI GCAGC 3 cut(s) 475, 613, 1444
BseYI CCCAGC 1 cut(s) 307
Bsh1236I CGCG 2 cut(s) 237, 501
Bsh1285I CGRYCG 1 cut(s) 157
BshFI GGCC 4 cut(s) 5, 89, 323, 655
BshNI GGYRCC 1 cut(s) 1741
BshVI ATCGAT 1 cut(s) 861
BsiEI CGRYCG 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 4 cut(s) 47, 108, 333, 341
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 6 cut(s) 319, 839, 995, 1154, 1507, 1948
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 4 cut(s) 5, 89, 323, 655
Bso31I GGTCTC 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 99, 693
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 9 cut(s) 53, 129, 148, 257, 516, 808, 858, 862, 1315
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 7 cut(s) 32, 124, 235, 271, 283, 501, 1739
BspANI GGCC 4 cut(s) 5, 89, 323, 655
BspCNI CTCAG 4 cut(s) 30, 1075, 1228, 1859
BspDI ATCGAT 1 cut(s) 861
BspEI TCCGGA 1 cut(s) 107
BspFNI CGCG 2 cut(s) 237, 501
BspHI TCATGA 1 cut(s) 1357
BspLI GGNNCC 4 cut(s) 36, 654, 1710, 1743
BspMAI CTGCAG 1 cut(s) 1722
BspPI GGATC 4 cut(s) 252, 816, 857, 866
BspT107I GGYRCC 1 cut(s) 1741
BspTI CTTAAG 1 cut(s) 1916
BspTNI GGTCTC 1 cut(s) 296
BsrDI GCAATG 3 cut(s) 498, 534, 1033
BsrI ACTGG 3 cut(s) 274, 313, 588
BssECI CCNNGG 5 cut(s) 84, 90, 244, 477, 833
BssMI GATC 9 cut(s) 53, 129, 148, 257, 516, 808, 858, 862, 1315
BssNI GRCGYC 2 cut(s) 138, 1742
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 3 cut(s) 84, 477, 833
Bst2BI CACGAG 1 cut(s) 597
Bst2UI CCWGG 2 cut(s) 92, 1502
Bst4CI ACNGT 7 cut(s) 158, 292, 1072, 1205, 1529, 1690, 1936
Bst6I CTCTTC 3 cut(s) 1527, 1601, 1794
BstACI GRCGYC 2 cut(s) 138, 1742
BstAFI CTTAAG 1 cut(s) 1916
BstAPI GCANNNNNTGC 1 cut(s) 68
BstBAI YACGTR 1 cut(s) 68
BstC8I GCNNGC 4 cut(s) 321, 705, 986, 1746
BstDEI CTNAG 5 cut(s) 17, 576, 1083, 1215, 1846
BstDSI CCRYGG 1 cut(s) 477
BstEII GGTNACC 2 cut(s) 79, 335
BstENI CCTNNNNNAGG 2 cut(s) 1152, 1946
BstF5I GGATG 4 cut(s) 165, 1018, 1405, 1585
BstFNI CGCG 2 cut(s) 237, 501
BstH2I RGCGCY 1 cut(s) 1745
BstHHI GCGC 4 cut(s) 299, 501, 1744, 1954
BstKTI GATC 9 cut(s) 56, 132, 151, 260, 519, 811, 861, 865, 1318
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 9 cut(s) 53, 129, 148, 257, 516, 808, 858, 862, 1315
BstMCI CGRYCG 1 cut(s) 157
BstMWI GCNNNNNNNGC 7 cut(s) 68, 635, 1085, 1109, 1466, 1745, 1927
BstNI CCWGG 2 cut(s) 92, 1502
BstNSI RCATGY 1 cut(s) 988
BstPI GGTNACC 2 cut(s) 79, 335
BstSCI CCNGG 3 cut(s) 90, 339, 1500
BstSFI CTRYAG 1 cut(s) 1718
BstUI CGCG 2 cut(s) 237, 501
BstV1I GCAGC 3 cut(s) 475, 613, 1444
BstV2I GAAGAC 2 cut(s) 356, 1892
BstXI CCANNNNNNTGG 1 cut(s) 91
Bsu15I ATCGAT 1 cut(s) 861
BsuI GTATCC 1 cut(s) 949
BsuRI GGCC 4 cut(s) 5, 89, 323, 655
BsuTUI ATCGAT 1 cut(s) 861
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 4 cut(s) 165, 1018, 1405, 1585
BtsI GCAGTG 1 cut(s) 628
BtsIMutI CAGTG 3 cut(s) 628, 1068, 1534
Cac8I GCNNGC 4 cut(s) 321, 705, 986, 1746
CciI TCATGA 1 cut(s) 1357
CfoI GCGC 4 cut(s) 299, 501, 1744, 1954
Cfr13I GGNCC 6 cut(s) 34, 49, 88, 322, 653, 1186
ClaI ATCGAT 1 cut(s) 861
CpoI CGGWCCG 1 cut(s) 49
CseI GACGC 2 cut(s) 127, 702
Csp6I GTAC 6 cut(s) 277, 921, 1320, 1464, 1694, 1937
CspI CGGWCCG 1 cut(s) 49
CviAII CATG 5 cut(s) 142, 478, 985, 1358, 1775
CviQI GTAC 6 cut(s) 277, 921, 1320, 1464, 1694, 1937
DdeI CTNAG 5 cut(s) 17, 576, 1083, 1215, 1846
DinI GGCGCC 1 cut(s) 1743
DpnI GATC 9 cut(s) 55, 131, 150, 259, 518, 810, 860, 864, 1317
DpnII GATC 9 cut(s) 53, 129, 148, 257, 516, 808, 858, 862, 1315
DraI TTTAAA 1 cut(s) 1591
Eam1104I CTCTTC 3 cut(s) 1527, 1601, 1794
EarI CTCTTC 3 cut(s) 1527, 1601, 1794
EciI GGCGGA 1 cut(s) 260
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 3 cut(s) 84, 477, 833
Eco24I GRGCYC 2 cut(s) 99, 693
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 3 cut(s) 34, 49, 1186
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 5 cut(s) 368, 954, 1314, 1497, 1635
Eco72I CACGTG 1 cut(s) 68
Eco91I GGTNACC 2 cut(s) 79, 335
EcoICRI GAGCTC 1 cut(s) 97
EcoNI CCTNNNNNAGG 2 cut(s) 1152, 1946
EcoO109I RGGNCCY 2 cut(s) 88, 322
EcoO65I GGTNACC 2 cut(s) 79, 335
EcoRI GAATTC 1 cut(s) 1636
EcoRII CCWGG 2 cut(s) 90, 1500
EcoT14I CCWWGG 3 cut(s) 84, 477, 833
EcoT38I GRGCYC 2 cut(s) 99, 693
EgeI GGCGCC 1 cut(s) 1743
EheI GGCGCC 1 cut(s) 1743
ErhI CCWWGG 3 cut(s) 84, 477, 833
FaeI CATG 5 cut(s) 145, 481, 988, 1361, 1778
FaqI GGGAC 1 cut(s) 236
FatI CATG 5 cut(s) 141, 477, 984, 1357, 1774
FauI CCCGC 1 cut(s) 290
FauNDI CATATG 2 cut(s) 523, 658
Fnu4HI GCNGC 4 cut(s) 489, 627, 1458, 1740
FokI GGATG 4 cut(s) 152, 1025, 1412, 1592
FriOI GRGCYC 2 cut(s) 99, 693
Fsp4HI GCNGC 4 cut(s) 489, 627, 1458, 1740
FspBI CTAG 4 cut(s) 686, 740, 873, 927
GlaI GCGC 4 cut(s) 298, 500, 1743, 1953
GluI GCNGC 4 cut(s) 489, 627, 1458, 1740
GsaI CCCAGC 1 cut(s) 311
GsuI CTGGAG 3 cut(s) 113, 1134, 1698
HaeII RGCGCY 1 cut(s) 1745
HaeIII GGCC 4 cut(s) 5, 89, 323, 655
HapII CCGG 4 cut(s) 47, 108, 333, 341
HgaI GACGC 2 cut(s) 127, 702
HhaI GCGC 4 cut(s) 299, 501, 1744, 1954
Hin1I GRCGYC 2 cut(s) 138, 1742
Hin1II CATG 5 cut(s) 145, 481, 988, 1361, 1778
Hin6I GCGC 4 cut(s) 297, 499, 1742, 1952
HinP1I GCGC 4 cut(s) 297, 499, 1742, 1952
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HpaII CCGG 4 cut(s) 47, 108, 333, 341
HphI GGTGA 5 cut(s) 91, 280, 854, 890, 896
Hpy166II GTNNAC 3 cut(s) 226, 279, 1186
Hpy8I GTNNAC 3 cut(s) 226, 279, 1186
HpyAV CCTTC 5 cut(s) 16, 195, 597, 875, 1942
HpyCH4III ACNGT 7 cut(s) 158, 292, 1072, 1205, 1529, 1690, 1936
HpyCH4IV ACGT 2 cut(s) 67, 1443
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 635, 1085, 1109, 1466, 1745, 1927
HpyF3I CTNAG 5 cut(s) 17, 576, 1083, 1215, 1846
HpySE526I ACGT 2 cut(s) 67, 1443
Hsp92I GRCGYC 2 cut(s) 138, 1742
Hsp92II CATG 5 cut(s) 145, 481, 988, 1361, 1778
HspAI GCGC 4 cut(s) 297, 499, 1742, 1952
KasI GGCGCC 1 cut(s) 1741
Kpn2I TCCGGA 1 cut(s) 107
Kzo9I GATC 9 cut(s) 53, 129, 148, 257, 516, 808, 858, 862, 1315
LmnI GCTCC 4 cut(s) 94, 496, 700, 1525
Lsp1109I GCAGC 3 cut(s) 475, 613, 1444
LweI GCATC 1 cut(s) 462
MaeI CTAG 4 cut(s) 686, 740, 873, 927
MaeII ACGT 2 cut(s) 67, 1443
MaeIII GTNAC 9 cut(s) 79, 335, 579, 842, 929, 990, 1105, 1381, 1789
MalI GATC 9 cut(s) 55, 131, 150, 259, 518, 810, 860, 864, 1317
MboI GATC 9 cut(s) 53, 129, 148, 257, 516, 808, 858, 862, 1315
MboII GAAGA 9 cut(s) 361, 481, 1155, 1544, 1618, 1628, 1811, 1897, 1901
MfeI CAATTG 1 cut(s) 1749
MhlI GDGCHC 2 cut(s) 99, 693
Mly113I GGCGCC 1 cut(s) 1742
MlyI GAGTC 4 cut(s) 589, 730, 1838, 1882
MroI TCCGGA 1 cut(s) 107
MslI CAYNNNNRTG 1 cut(s) 1375
MspA1I CMGCKG 1 cut(s) 1103
MspCI CTTAAG 1 cut(s) 1916
MspI CCGG 4 cut(s) 47, 108, 333, 341
MspR9I CCNGG 3 cut(s) 92, 341, 1502
MunI CAATTG 1 cut(s) 1749
MvaI CCWGG 2 cut(s) 92, 1502
MvnI CGCG 2 cut(s) 237, 501
MwoI GCNNNNNNNGC 7 cut(s) 68, 635, 1085, 1109, 1466, 1745, 1927
NarI GGCGCC 1 cut(s) 1742
NciI CCSGG 1 cut(s) 341
NcoI CCATGG 1 cut(s) 477
NdeI CATATG 2 cut(s) 523, 658
NdeII GATC 9 cut(s) 53, 129, 148, 257, 516, 808, 858, 862, 1315
NlaIII CATG 5 cut(s) 145, 481, 988, 1361, 1778
NlaIV GGNNCC 4 cut(s) 36, 654, 1710, 1743
NmeAIII GCCGAG 2 cut(s) 78, 325
NmuCI GTSAC 6 cut(s) 79, 842, 990, 1105, 1381, 1789
NspI RCATGY 1 cut(s) 988
OliI CACNNNNGTG 1 cut(s) 1375
PaeI GCATGC 1 cut(s) 988
PagI TCATGA 1 cut(s) 1357
PfeI GAWTC 6 cut(s) 111, 382, 529, 607, 1505, 1673
PkrI GCNGC 4 cut(s) 490, 628, 1459, 1741
PleI GAGTC 4 cut(s) 589, 730, 1838, 1882
PluTI GGCGCC 1 cut(s) 1745
PmaCI CACGTG 1 cut(s) 68
PmlI CACGTG 1 cut(s) 68
PpsI GAGTC 4 cut(s) 589, 730, 1838, 1882
Ppu21I YACGTR 1 cut(s) 68
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 2 cut(s) 90, 1500
PspCI CACGTG 1 cut(s) 68
PspEI GGTNACC 2 cut(s) 79, 335
PspFI CCCAGC 1 cut(s) 307
PspGI CCWGG 2 cut(s) 90, 1500
PspN4I GGNNCC 4 cut(s) 36, 654, 1710, 1743
PspPI GGNCC 6 cut(s) 34, 49, 88, 322, 653, 1186
PstI CTGCAG 1 cut(s) 1722
PvuII CAGCTG 1 cut(s) 1103
RsaI GTAC 6 cut(s) 278, 922, 1321, 1465, 1695, 1938
RsaNI GTAC 6 cut(s) 277, 921, 1320, 1464, 1694, 1937
RseI CAYNNNNRTG 1 cut(s) 1375
Rsr2I CGGWCCG 1 cut(s) 49
RsrII CGGWCCG 1 cut(s) 49
SacI GAGCTC 1 cut(s) 99
SatI GCNGC 4 cut(s) 489, 627, 1458, 1740
Sau3AI GATC 9 cut(s) 53, 129, 148, 257, 516, 808, 858, 862, 1315
Sau96I GGNCC 6 cut(s) 34, 49, 88, 322, 653, 1186
SchI GAGTC 4 cut(s) 589, 730, 1838, 1882
ScrFI CCNGG 3 cut(s) 92, 341, 1502
SduI GDGCHC 2 cut(s) 99, 693
SfaNI GCATC 1 cut(s) 462
SfcI CTRYAG 1 cut(s) 1718
SfoI GGCGCC 1 cut(s) 1743
SinI GGWCC 3 cut(s) 34, 49, 1186
SmiMI CAYNNNNRTG 1 cut(s) 1375
SmlI CTYRAG 3 cut(s) 194, 1916, 1943
SmoI CTYRAG 3 cut(s) 194, 1916, 1943
SphI GCATGC 1 cut(s) 988
SsiI CCGC 7 cut(s) 32, 124, 235, 271, 283, 501, 1739
SspDI GGCGCC 1 cut(s) 1741
SspI AATATT 1 cut(s) 1333
SspMI CTAG 4 cut(s) 686, 740, 873, 927
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 3 cut(s) 90, 339, 1500
StyI CCWWGG 3 cut(s) 84, 477, 833
TaaI ACNGT 7 cut(s) 158, 292, 1072, 1205, 1529, 1690, 1936
TaiI ACGT 2 cut(s) 70, 1446
TaqI TCGA 5 cut(s) 12, 396, 463, 811, 861
TaqII GACCGA 1 cut(s) 66
TatI WGTACW 3 cut(s) 276, 1463, 1693
TauI GCSGC 1 cut(s) 1742
TfiI GAWTC 6 cut(s) 111, 382, 529, 607, 1505, 1673
TscAI CASTG 3 cut(s) 628, 1075, 1534
TseFI GTSAC 6 cut(s) 79, 842, 990, 1105, 1381, 1789
TseI GCWGC 3 cut(s) 488, 626, 1457
Tsp45I GTSAC 6 cut(s) 79, 842, 990, 1105, 1381, 1789
TspDTI ATGAA 5 cut(s) 657, 1218, 1346, 1596, 1629
TspGWI ACGGA 1 cut(s) 211
TspRI CASTG 3 cut(s) 628, 1075, 1534
Vha464I CTTAAG 1 cut(s) 1916
VpaK11BI GGWCC 3 cut(s) 34, 49, 1186
XagI CCTNNNNNAGG 2 cut(s) 1152, 1946
XapI RAATTY 4 cut(s) 644, 1353, 1584, 1636
XceI RCATGY 1 cut(s) 988
XspI CTAG 4 cut(s) 686, 740, 873, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.