pycom11g20390

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006631
Physical Location & Seq
Reverse (-)
23315740 .. 23317491
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 939 bp
ATGGGAGGTGGCGGAGTCGTGAGGTCGGTAGCAAAGGTCGCCGGGATCGGCGGCGTTGTCCACGGTGGCATGCTCCGCGGCGCGCCGGGCGTTTCTTCGCCGACTGGCCATTCGGTCCGCAAGGCCTCAATTCCCGTGGCGGCGACCCTCACGGCTCAGAATAACGGCGGAGCTGAAGTGGCGGCGATCGAGAAGCTTGCGTGGGACGCTGGCGACGAATTTGTCGACTGGCAGGTGGTCGGCGCCGGAGAAGATGATCTGGTGATGTCGGGTGGCGAGCCGATGCCGAGGGTTGTCTTCGACGCTGCGCCGAGCTTCAAGGAGGCCAAAGAGGCCACCGCTGAATTGAAAGACGCCCTCGATAAGGTATATCTTTCTTCACCCAAATCTACTGATTTTGGGGAAGTGTCTGCTGCTGATAATGTGGCTGGACTGTCTCTGATCACACACCCTGAACCTGAGGAGATGGAATCCCTACTTCTTACTAGGACTTCGGTGCCAAAACATGCTCTTCATGCATTTGAAATGCTGAGTCGAAGTTCTGAAGCTCAGAATGTTGTTGCTTCAATTGCAAGTGATCCGAATATCTGGAATGCCATGATGGAAAATTCCGCGGTCAAGCAATTTATGGCCAACAAGAACTATAATCGTTACATGGAAGAATATGCAGCAGATACCTATCCAGTTTCACACAAGAAGCTTGAAGACGAATCAGAGAAATCTGACTCCTTGGAAGATATGCTCGGTGGTTTTGTGCAGAAAATCAAGCTGACAGTGGAGAAGTGGGTCAGTGATTTGTCGTCATTCATCCAGAACATTTTCGAACCCCCTGCAGCTGAGACTAAAGCGGATGGAGGGAATACAAAGTCAGTTGTAGCATCTGCATTCATGGGACTAGCAGTTATGGTTGTTATGGTGGTCTTGCTGAAGCGTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

33.08

Weight (kDa)

4.91

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 223
AasI GACNNNNNNGTC 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 223
AccB1I GGYRCC 2 cut(s) 242, 496
AccI GTMKAC 1 cut(s) 225
AccII CGCG 3 cut(s) 78, 83, 614
AclWI GGATC 2 cut(s) 53, 572
AcoI YGGCCR 2 cut(s) 106, 630
AcsI RAATTY 2 cut(s) 218, 607
AcuI CTGAAG 2 cut(s) 195, 564
AcyI GRCGYC 2 cut(s) 243, 354
AfiI CCNNNNNNNGG 1 cut(s) 364
AgsI TTSAA 5 cut(s) 319, 349, 524, 567, 704
AluBI AGCT 7 cut(s) 173, 196, 315, 548, 700, 769, 836
AluI AGCT 7 cut(s) 173, 196, 315, 548, 700, 769, 836
Alw26I GTCTC 2 cut(s) 441, 833
AlwI GGATC 2 cut(s) 53, 572
AoxI GGCC 5 cut(s) 106, 123, 324, 333, 630
ApeKI GCWGC 4 cut(s) 305, 413, 668, 833
ApoI RAATTY 2 cut(s) 218, 607
ArsI GACNNNNNNTTYG 2 cut(s) 94, 126
AscI GGCGCGCC 1 cut(s) 81
Asp700I GAANNNNTTC 1 cut(s) 818
AspLEI GCGC 4 cut(s) 83, 85, 245, 310
AspS9I GGNCC 1 cut(s) 115
AsuC2I CCSGG 2 cut(s) 43, 87
AsuHPI GGTGA 2 cut(s) 274, 372
AsuII TTCGAA 1 cut(s) 822
AvaII GGWCC 1 cut(s) 115
AxyI CCTNAGG 1 cut(s) 459
BalI TGGCCA 2 cut(s) 108, 632
BanI GGYRCC 2 cut(s) 242, 496
BbsI GAAGAC 2 cut(s) 289, 711
BbvI GCAGC 4 cut(s) 292, 400, 680, 845
BccI CCATC 3 cut(s) 460, 595, 845
BceAI ACGGC 2 cut(s) 168, 181
BcgI CGANNNNNNTGC 4 cut(s) 179, 213, 812, 846
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 2 cut(s) 43, 87
BcoDI GTCTC 2 cut(s) 441, 833
BfaI CTAG 3 cut(s) 486, 896, 937
BfmI CTRYAG 1 cut(s) 831
BfoI RGCGCY 1 cut(s) 246
BfuAI ACCTGC 1 cut(s) 223
BglI GCCNNNNNGGC 1 cut(s) 332
BisI GCNGC 8 cut(s) 52, 79, 141, 183, 306, 414, 669, 834
BlsI GCNGC 8 cut(s) 53, 80, 142, 184, 307, 415, 670, 835
Bme1390I CCNGG 2 cut(s) 43, 87
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 2 cut(s) 244, 498
BmrFI CCNGG 2 cut(s) 43, 87
BmsI GCATC 2 cut(s) 273, 887
BpiI GAAGAC 2 cut(s) 289, 711
Bpu14I TTCGAA 1 cut(s) 822
BpuMI CCSGG 2 cut(s) 43, 87
BsaBI GATNNNNATC 1 cut(s) 678
BsaHI GRCGYC 2 cut(s) 243, 354
BsaJI CCNNGG 6 cut(s) 61, 76, 135, 287, 612, 729
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 1 cut(s) 364
Bse1I ACTGG 3 cut(s) 109, 233, 683
Bse21I CCTNAGG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 678
BseDI CCNNGG 6 cut(s) 61, 76, 135, 287, 612, 729
BseGI GGATG 2 cut(s) 807, 856
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 1 cut(s) 364
BseMII CTCAG 5 cut(s) 170, 450, 521, 563, 828
BseNI ACTGG 3 cut(s) 109, 233, 683
BsePI GCGCGC 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 4 cut(s) 292, 400, 680, 845
BsgI GTGCAG 1 cut(s) 776
Bsh1236I CGCG 3 cut(s) 78, 83, 614
Bsh1285I CGRYCG 1 cut(s) 189
BshFI GGCC 5 cut(s) 108, 125, 326, 335, 632
BshNI GGYRCC 2 cut(s) 242, 496
BsiEI CGRYCG 1 cut(s) 189
BsiSI CCGG 3 cut(s) 42, 86, 246
BslFI GGGAC 2 cut(s) 218, 906
BslI CCNNNNNNNGG 1 cut(s) 364
BsmAI GTCTC 2 cut(s) 441, 833
BsmFI GGGAC 2 cut(s) 218, 906
BsmI GAATGC 2 cut(s) 598, 884
BsnI GGCC 5 cut(s) 108, 125, 326, 335, 632
Bsp119I TTCGAA 1 cut(s) 822
Bsp143I GATC 5 cut(s) 45, 186, 256, 441, 577
BspANI GGCC 5 cut(s) 108, 125, 326, 335, 632
BspCNI CTCAG 5 cut(s) 169, 451, 522, 562, 829
BspFNI CGCG 3 cut(s) 78, 83, 614
BspLI GGNNCC 2 cut(s) 244, 498
BspMAI CTGCAG 1 cut(s) 835
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 2 cut(s) 53, 572
BspQI GCTCTTC 1 cut(s) 516
BspT104I TTCGAA 1 cut(s) 822
BspT107I GGYRCC 2 cut(s) 242, 496
BsrI ACTGG 3 cut(s) 109, 233, 683
BssECI CCNNGG 6 cut(s) 61, 76, 135, 287, 612, 729
BssHII GCGCGC 1 cut(s) 81
BssMI GATC 5 cut(s) 45, 186, 256, 441, 577
BssNI GRCGYC 2 cut(s) 243, 354
BssT1I CCWWGG 1 cut(s) 729
Bst4CI ACNGT 3 cut(s) 65, 435, 775
Bst6I CTCTTC 1 cut(s) 516
BstACI GRCGYC 2 cut(s) 243, 354
BstBI TTCGAA 1 cut(s) 822
BstC8I GCNNGC 6 cut(s) 71, 83, 198, 211, 278, 933
BstDEI CTNAG 5 cut(s) 156, 459, 530, 549, 837
BstDSI CCRYGG 4 cut(s) 61, 76, 135, 612
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 2 cut(s) 807, 856
BstFNI CGCG 3 cut(s) 78, 83, 614
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 4 cut(s) 83, 85, 245, 310
BstKTI GATC 5 cut(s) 48, 189, 259, 444, 580
BstMAI GTCTC 2 cut(s) 441, 833
BstMBI GATC 5 cut(s) 45, 186, 256, 441, 577
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 8 cut(s) 38, 75, 87, 179, 206, 332, 515, 569
BstNSI RCATGY 2 cut(s) 73, 509
BstSCI CCNGG 2 cut(s) 41, 85
BstSFI CTRYAG 1 cut(s) 831
BstUI CGCG 3 cut(s) 78, 83, 614
BstV1I GCAGC 4 cut(s) 292, 400, 680, 845
BstV2I GAAGAC 2 cut(s) 289, 711
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 5 cut(s) 108, 125, 326, 335, 632
BtgI CCRYGG 4 cut(s) 61, 76, 135, 612
BtsCI GGATG 2 cut(s) 807, 856
BtsIMutI CAGTG 2 cut(s) 780, 796
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 6 cut(s) 71, 83, 198, 211, 278, 933
CfoI GCGC 4 cut(s) 83, 85, 245, 310
Cfr13I GGNCC 1 cut(s) 115
Cfr42I CCGCGG 2 cut(s) 79, 615
CpoI CGGWCCG 1 cut(s) 115
CseI GACGC 3 cut(s) 215, 311, 362
CspCI CAANNNNNGTGG 2 cut(s) 117, 152
CspI CGGWCCG 1 cut(s) 115
CviAII CATG 6 cut(s) 70, 506, 515, 598, 655, 889
DdeI CTNAG 5 cut(s) 156, 459, 530, 549, 837
DinI GGCGCC 1 cut(s) 244
DpnI GATC 5 cut(s) 47, 188, 258, 443, 579
DpnII GATC 5 cut(s) 45, 186, 256, 441, 577
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 2 cut(s) 106, 630
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
EciI GGCGGA 2 cut(s) 27, 183
Eco130I CCWWGG 1 cut(s) 729
Eco147I AGGCCT 1 cut(s) 125
Eco47I GGWCC 1 cut(s) 115
Eco57I CTGAAG 2 cut(s) 195, 564
Eco81I CCTNAGG 1 cut(s) 459
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoT14I CCWWGG 1 cut(s) 729
EcoT22I ATGCAT 1 cut(s) 520
EgeI GGCGCC 1 cut(s) 244
EheI GGCGCC 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 729
FaeI CATG 6 cut(s) 73, 509, 518, 601, 658, 892
FaqI GGGAC 2 cut(s) 218, 906
FatI CATG 6 cut(s) 69, 505, 514, 597, 654, 888
FbaI TGATCA 1 cut(s) 441
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 8 cut(s) 52, 79, 141, 183, 306, 414, 669, 834
FokI GGATG 2 cut(s) 794, 863
Fsp4HI GCNGC 8 cut(s) 52, 79, 141, 183, 306, 414, 669, 834
FspBI CTAG 3 cut(s) 486, 896, 937
GlaI GCGC 4 cut(s) 82, 84, 244, 309
GluI GCNGC 8 cut(s) 52, 79, 141, 183, 306, 414, 669, 834
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 5 cut(s) 108, 125, 326, 335, 632
HapII CCGG 3 cut(s) 42, 86, 246
HgaI GACGC 3 cut(s) 215, 311, 362
HhaI GCGC 4 cut(s) 83, 85, 245, 310
Hin1I GRCGYC 2 cut(s) 243, 354
Hin1II CATG 6 cut(s) 73, 509, 518, 601, 658, 892
Hin6I GCGC 4 cut(s) 81, 83, 243, 308
HinP1I GCGC 4 cut(s) 81, 83, 243, 308
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 2 cut(s) 194, 698
HinfI GANTC 5 cut(s) 15, 470, 532, 710, 725
HpaII CCGG 3 cut(s) 42, 86, 246
HphI GGTGA 2 cut(s) 274, 372
Hpy166II GTNNAC 2 cut(s) 61, 226
Hpy188I TCNGA 7 cut(s) 159, 441, 544, 552, 582, 715, 724
Hpy188III TCNNGA 4 cut(s) 19, 190, 589, 811
Hpy8I GTNNAC 2 cut(s) 61, 226
Hpy99I CGWCG 2 cut(s) 218, 305
HpyCH4III ACNGT 3 cut(s) 65, 435, 775
HpyCH4V TGCA 6 cut(s) 518, 572, 668, 757, 833, 884
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 75, 87, 179, 206, 332, 515, 569
HpyF3I CTNAG 5 cut(s) 156, 459, 530, 549, 837
Hsp92I GRCGYC 2 cut(s) 243, 354
Hsp92II CATG 6 cut(s) 73, 509, 518, 601, 658, 892
HspAI GCGC 4 cut(s) 81, 83, 243, 308
KasI GGCGCC 1 cut(s) 242
Ksp22I TGATCA 1 cut(s) 441
KspI CCGCGG 2 cut(s) 79, 615
Kzo9I GATC 5 cut(s) 45, 186, 256, 441, 577
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 2 cut(s) 78, 170
Lsp1109I GCAGC 4 cut(s) 292, 400, 680, 845
LweI GCATC 2 cut(s) 273, 887
MaeI CTAG 3 cut(s) 486, 896, 937
MaeIII GTNAC 1 cut(s) 650
MalI GATC 5 cut(s) 47, 188, 258, 443, 579
MboI GATC 5 cut(s) 45, 186, 256, 441, 577
MboII GAAGA 8 cut(s) 87, 263, 289, 369, 503, 671, 716, 746
MfeI CAATTG 1 cut(s) 567
MlsI TGGCCA 2 cut(s) 108, 632
MluCI AATT 6 cut(s) 129, 218, 344, 567, 607, 623
MluNI TGGCCA 2 cut(s) 108, 632
Mly113I GGCGCC 1 cut(s) 243
MlyI GAGTC 3 cut(s) 24, 541, 719
MnlI CCTC 9 cut(s) 15, 136, 158, 282, 316, 325, 368, 454, 848
Mox20I TGGCCA 2 cut(s) 108, 632
Mph1103I ATGCAT 1 cut(s) 520
MroXI GAANNNNTTC 1 cut(s) 818
MscI TGGCCA 2 cut(s) 108, 632
Msp20I TGGCCA 2 cut(s) 108, 632
MspA1I CMGCKG 4 cut(s) 78, 341, 614, 836
MspI CCGG 3 cut(s) 42, 86, 246
MspR9I CCNGG 2 cut(s) 43, 87
MunI CAATTG 1 cut(s) 567
Mva1269I GAATGC 2 cut(s) 598, 884
MvnI CGCG 3 cut(s) 78, 83, 614
MwoI GCNNNNNNNGC 8 cut(s) 38, 75, 87, 179, 206, 332, 515, 569
NarI GGCGCC 1 cut(s) 243
NciI CCSGG 2 cut(s) 43, 87
NdeII GATC 5 cut(s) 45, 186, 256, 441, 577
NlaIII CATG 6 cut(s) 73, 509, 518, 601, 658, 892
NlaIV GGNNCC 2 cut(s) 244, 498
NmeAIII GCCGAG 2 cut(s) 312, 336
NsiI ATGCAT 1 cut(s) 520
NspI RCATGY 2 cut(s) 73, 509
NspV TTCGAA 1 cut(s) 822
PaeI GCATGC 1 cut(s) 73
PalAI GGCGCGCC 1 cut(s) 81
PaqCI CACCTGC 1 cut(s) 223
PauI GCGCGC 1 cut(s) 81
PceI AGGCCT 1 cut(s) 125
PciSI GCTCTTC 1 cut(s) 516
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 222
PctI GAATGC 2 cut(s) 598, 884
PdmI GAANNNNTTC 1 cut(s) 818
PfeI GAWTC 2 cut(s) 470, 710
PkrI GCNGC 8 cut(s) 53, 80, 142, 184, 307, 415, 670, 835
Ple19I CGATCG 1 cut(s) 189
PleI GAGTC 3 cut(s) 23, 540, 719
PluTI GGCGCC 1 cut(s) 246
PpsI GAGTC 3 cut(s) 23, 540, 719
PspN4I GGNNCC 2 cut(s) 244, 498
PspPI GGNCC 1 cut(s) 115
PstI CTGCAG 1 cut(s) 835
PteI GCGCGC 1 cut(s) 81
PvuI CGATCG 1 cut(s) 189
PvuII CAGCTG 1 cut(s) 836
Rsr2I CGGWCCG 1 cut(s) 115
RsrII CGGWCCG 1 cut(s) 115
SacII CCGCGG 2 cut(s) 79, 615
SalI GTCGAC 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 516
SatI GCNGC 8 cut(s) 52, 79, 141, 183, 306, 414, 669, 834
Sau3AI GATC 5 cut(s) 45, 186, 256, 441, 577
Sau96I GGNCC 1 cut(s) 115
SchI GAGTC 3 cut(s) 24, 541, 719
ScrFI CCNGG 2 cut(s) 43, 87
SfaNI GCATC 2 cut(s) 273, 887
SfcI CTRYAG 1 cut(s) 831
SfiI GGCCNNNNNGGCC 1 cut(s) 332
SfoI GGCGCC 1 cut(s) 244
Sfr303I CCGCGG 2 cut(s) 79, 615
SfuI TTCGAA 1 cut(s) 822
SgrBI CCGCGG 2 cut(s) 79, 615
SgsI GGCGCGCC 1 cut(s) 81
SinI GGWCC 1 cut(s) 115
SphI GCATGC 1 cut(s) 73
Sse9I AATT 6 cut(s) 129, 218, 344, 567, 607, 623
SseBI AGGCCT 1 cut(s) 125
SspDI GGCGCC 1 cut(s) 242
SspMI CTAG 3 cut(s) 486, 896, 937
StuI AGGCCT 1 cut(s) 125
StyD4I CCNGG 2 cut(s) 41, 85
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 3 cut(s) 65, 435, 775
TaqI TCGA 6 cut(s) 189, 225, 300, 360, 535, 822
TaqII GACCGA 1 cut(s) 103
TasI AATT 6 cut(s) 129, 218, 344, 567, 607, 623
TauI GCSGC 4 cut(s) 54, 81, 143, 185
TfiI GAWTC 2 cut(s) 470, 710
TscAI CASTG 2 cut(s) 780, 796
TseI GCWGC 4 cut(s) 305, 413, 668, 833
TspDTI ATGAA 3 cut(s) 503, 796, 877
TspRI CASTG 2 cut(s) 780, 796
VpaK11BI GGWCC 1 cut(s) 115
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 2 cut(s) 218, 607
XceI RCATGY 2 cut(s) 73, 509
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 818
XspI CTAG 3 cut(s) 486, 896, 937
Zsp2I ATGCAT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.