pycom11g26950

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003757
Physical Location & Seq
Forward (+)
29389341 .. 29389847
507 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 507 bp
ATGTCACGCACAGGAATTATTGCCGTCATCGTCCTCCTATCAACGTTCTCCACGTCACCAACTCTCTCCGCCGCTGCCAACGGCGCCGAATCGCGGTCTGTGTACGACGCAATCGAAGACTTTAACTTGCCGATGGGTCTGCTCCCGAAGGGCGTCGCCGGATACGAGCTCGACGGCAACGGAAAATTCCGCGCGTATCTGAACGGCTCCTGCCGCTTCTCGCTGGAAGGGTCGTACCAGCTCAATTACAAGTCCACTATTACCGGCACGATCTCCCAGAACAAGCTCTCCTCGCTGACCGGGGTGAGCGTTAAGGTCCTGTTCCTGTGGCTCAACATCGTCGAGGTCACCCGGAGGGGCGACAACCTCGAGTTCTCGGTGGGGATCGCGTCGGCGGCGTTTTCGATCGACAATTTCTATGAGTGCCCGCAGTGCGGCTGCGGGTTGGATTGCGACAATGGCGGACAAGTAAGGAAGATTAAAATGCCTCTTGTTTCTTCGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

169

Amino Acids

17.98

Weight (kDa)

6.24

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 34 - 139 1.9e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AccII CGCG 4 cut(s) 94, 192, 194, 389
AclI AACGTT 1 cut(s) 44
AclWI GGATC 1 cut(s) 392
AcsI RAATTY 1 cut(s) 185
AcyI GRCGYC 2 cut(s) 84, 153
AfaI GTAC 2 cut(s) 104, 236
AfiI CCNNNNNNNGG 2 cut(s) 93, 434
AjiI CACGTC 1 cut(s) 54
AloI GAACNNNNNNTCC 2 cut(s) 272, 304
AluBI AGCT 3 cut(s) 169, 241, 286
AluI AGCT 3 cut(s) 169, 241, 286
Alw21I GWGCWC 1 cut(s) 171
AlwI GGATC 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 368
ApeKI GCWGC 2 cut(s) 74, 438
ApoI RAATTY 1 cut(s) 185
AspLEI GCGC 2 cut(s) 86, 194
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 2 cut(s) 301, 352
AsuHPI GGTGA 3 cut(s) 48, 316, 340
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 428
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 61, 425
BccI CCATC 1 cut(s) 127
BceAI ACGGC 4 cut(s) 8, 97, 190, 220
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciVI GTATCC 1 cut(s) 155
BcnI CCSGG 2 cut(s) 301, 352
BfoI RGCGCY 1 cut(s) 87
BfuI GTATCC 1 cut(s) 155
BisI GCNGC 6 cut(s) 72, 75, 214, 396, 436, 439
BlsI GCNGC 6 cut(s) 73, 76, 215, 397, 437, 440
Bme1390I CCNGG 2 cut(s) 301, 352
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 368
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 2 cut(s) 85, 208
BmrFI CCNGG 2 cut(s) 301, 352
BpiI GAAGAC 1 cut(s) 123
BpuMI CCSGG 2 cut(s) 301, 352
BsaHI GRCGYC 2 cut(s) 84, 153
BsaJI CCNNGG 1 cut(s) 300
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 434
Bse118I RCCGGY 1 cut(s) 263
BseDI CCNNGG 1 cut(s) 300
BseLI CCNNNNNNNGG 2 cut(s) 93, 434
BseRI GAGGAG 1 cut(s) 280
BseSI GKGCMC 1 cut(s) 428
BseXI GCAGC 2 cut(s) 61, 425
Bsh1236I CGCG 4 cut(s) 94, 192, 194, 389
Bsh1285I CGRYCG 1 cut(s) 408
BshNI GGYRCC 1 cut(s) 83
BsiEI CGRYCG 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 4 cut(s) 159, 264, 300, 352
BslI CCNNNNNNNGG 2 cut(s) 93, 434
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 171, 428
Bsp143I GATC 3 cut(s) 270, 384, 405
BspFNI CGCG 4 cut(s) 94, 192, 194, 389
BspLI GGNNCC 2 cut(s) 85, 208
BspPI GGATC 1 cut(s) 392
BspT107I GGYRCC 1 cut(s) 83
BsrFI RCCGGY 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 3 cut(s) 270, 384, 405
BssNI GRCGYC 2 cut(s) 84, 153
BstACI GRCGYC 2 cut(s) 84, 153
BstC8I GCNNGC 1 cut(s) 428
BstEII GGTNACC 1 cut(s) 346
BstFNI CGCG 4 cut(s) 94, 192, 194, 389
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 2 cut(s) 86, 194
BstKTI GATC 3 cut(s) 273, 387, 408
BstMBI GATC 3 cut(s) 270, 384, 405
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 6 cut(s) 83, 213, 292, 395, 432, 459
BstPI GGTNACC 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 299, 350
BstSLI GKGCMC 1 cut(s) 428
BstUI CGCG 4 cut(s) 94, 192, 194, 389
BstV1I GCAGC 2 cut(s) 61, 425
BstV2I GAAGAC 1 cut(s) 123
BsuI GTATCC 1 cut(s) 155
BtrI CACGTC 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 437
BtsIMutI CAGTG 1 cut(s) 437
Cac8I GCNNGC 1 cut(s) 428
CfoI GCGC 2 cut(s) 86, 194
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 3 cut(s) 116, 142, 378
Csp6I GTAC 2 cut(s) 103, 235
CviJI RGCY 6 cut(s) 169, 207, 241, 286, 331, 438
CviKI_1 RGCY 6 cut(s) 169, 207, 241, 286, 331, 438
CviQI GTAC 2 cut(s) 103, 235
DinI GGCGCC 1 cut(s) 85
DpnI GATC 3 cut(s) 272, 386, 407
DpnII GATC 3 cut(s) 270, 384, 405
EciI GGCGGA 2 cut(s) 58, 477
Ecl136II GAGCTC 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 169
Eco88I CYCGRG 1 cut(s) 368
Eco91I GGTNACC 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 316
EcoO65I GGTNACC 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 171
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
FaiI YATR 1 cut(s) 420
FauI CCCGC 2 cut(s) 434, 435
Fnu4HI GCNGC 6 cut(s) 72, 75, 214, 396, 436, 439
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 6 cut(s) 72, 75, 214, 396, 436, 439
GlaI GCGC 2 cut(s) 85, 193
GluI GCNGC 6 cut(s) 72, 75, 214, 396, 436, 439
HaeII RGCGCY 1 cut(s) 87
HapII CCGG 4 cut(s) 159, 264, 300, 352
HgaI GACGC 3 cut(s) 116, 142, 378
HhaI GCGC 2 cut(s) 86, 194
Hin1I GRCGYC 2 cut(s) 84, 153
Hin6I GCGC 2 cut(s) 84, 192
HinP1I GCGC 2 cut(s) 84, 192
HinfI GANTC 1 cut(s) 89
HpaII CCGG 4 cut(s) 159, 264, 300, 352
HphI GGTGA 3 cut(s) 48, 316, 340
Hpy166II GTNNAC 2 cut(s) 103, 255
Hpy188I TCNGA 1 cut(s) 201
Hpy188III TCNNGA 1 cut(s) 145
Hpy8I GTNNAC 2 cut(s) 103, 255
Hpy99I CGWCG 5 cut(s) 110, 158, 176, 344, 394
HpyAV CCTTC 2 cut(s) 142, 221
HpyCH4IV ACGT 2 cut(s) 44, 53
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 213, 292, 395, 432, 459
HpySE526I ACGT 2 cut(s) 44, 53
Hsp92I GRCGYC 2 cut(s) 84, 153
HspAI GCGC 2 cut(s) 84, 192
KasI GGCGCC 1 cut(s) 83
Kzo9I GATC 3 cut(s) 270, 384, 405
LmnI GCTCC 2 cut(s) 147, 212
Lsp1109I GCAGC 2 cut(s) 61, 425
MaeII ACGT 2 cut(s) 44, 53
MaeIII GTNAC 3 cut(s) 3, 54, 346
MalI GATC 3 cut(s) 272, 386, 407
MboI GATC 3 cut(s) 270, 384, 405
MboII GAAGA 3 cut(s) 128, 487, 489
MhlI GDGCHC 2 cut(s) 171, 428
MluCI AATT 4 cut(s) 15, 185, 244, 412
Mly113I GGCGCC 1 cut(s) 84
MmeI TCCRAC 1 cut(s) 426
MnlI CCTC 6 cut(s) 44, 301, 337, 348, 377, 498
MseI TTAA 3 cut(s) 123, 312, 480
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 4 cut(s) 159, 264, 300, 352
MspR9I CCNGG 2 cut(s) 301, 352
MvnI CGCG 4 cut(s) 94, 192, 194, 389
MwoI GCNNNNNNNGC 6 cut(s) 83, 213, 292, 395, 432, 459
NarI GGCGCC 1 cut(s) 84
NciI CCSGG 2 cut(s) 301, 352
NdeII GATC 3 cut(s) 270, 384, 405
NlaIV GGNNCC 2 cut(s) 85, 208
NmuCI GTSAC 3 cut(s) 3, 54, 346
PaeR7I CTCGAG 1 cut(s) 368
PcsI WCGNNNNNNNCGW 3 cut(s) 50, 111, 162
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 6 cut(s) 73, 76, 215, 397, 437, 440
Ple19I CGATCG 1 cut(s) 408
PluTI GGCGCC 1 cut(s) 87
PpuMI RGGWCCY 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 171
Psp1406I AACGTT 1 cut(s) 44
Psp5II RGGWCCY 1 cut(s) 316
PspEI GGTNACC 1 cut(s) 346
PspN4I GGNNCC 2 cut(s) 85, 208
PspPI GGNCC 1 cut(s) 316
PspPPI RGGWCCY 1 cut(s) 316
PspXI VCTCGAGB 1 cut(s) 368
PvuI CGATCG 1 cut(s) 408
RsaI GTAC 2 cut(s) 104, 236
RsaNI GTAC 2 cut(s) 103, 235
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 3 cut(s) 123, 312, 480
SatI GCNGC 6 cut(s) 72, 75, 214, 396, 436, 439
Sau3AI GATC 3 cut(s) 270, 384, 405
Sau96I GGNCC 1 cut(s) 316
ScrFI CCNGG 2 cut(s) 301, 352
SduI GDGCHC 2 cut(s) 171, 428
SetI ASST 8 cut(s) 47, 56, 171, 243, 288, 318, 348, 369
SfoI GGCGCC 1 cut(s) 85
Sfr274I CTCGAG 1 cut(s) 368
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 4 cut(s) 15, 185, 244, 412
SspDI GGCGCC 1 cut(s) 83
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 2 cut(s) 299, 350
TaiI ACGT 2 cut(s) 47, 56
TaqI TCGA 7 cut(s) 114, 171, 342, 369, 404, 408, 500
TasI AATT 4 cut(s) 15, 185, 244, 412
TauI GCSGC 4 cut(s) 74, 216, 398, 438
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 3 cut(s) 123, 312, 480
Tru9I TTAA 3 cut(s) 123, 312, 480
TscAI CASTG 1 cut(s) 437
TseFI GTSAC 3 cut(s) 3, 54, 346
TseI GCWGC 2 cut(s) 74, 438
Tsp45I GTSAC 3 cut(s) 3, 54, 346
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 1 cut(s) 185
XhoI CTCGAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.