pycom12g17640

Transmembrane protein 87B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003014
Physical Location & Seq
Reverse (-)
20036399 .. 20039182
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1704 bp
ATGAGGCCTAAGACGCCTTTGGACAGCGAAAAGACGGGCGGCGTCGTTTTCGTTTGGTTTGTCTTGTTTCTTACCCACTTCACCATCTCAAACCGCGCTGAGATTCTCGAGGGGGAAGAAGGTGAGAGAAAAATGGAACAGAGAAGGTTCTGGTGTGAAAAGGGCTCGCTCGTAGTGTTAATTTTGGGGGTTTTTCTCACATGCAACGAGTGCATCATAAGGTCTGATGCTTCAATCCACGAGTACAGAAATGAAGCTTTCAATCCTCAGTCAAACGCCTTCTTCTTCCATGGCGGCAGTGAAGGCCTCTACGCTTCCAAGGTCCACGATTCTACCGATAGTTCTTCAGCAGACAATCGCCACCTCAAGGGCAAGTCCTTCATCAAGTTTGAGAGTGTCACTTTTGTGAGGACGAAAGAGTCTGCGAATAAGATGAGTGAAATGCAGCAGGACACTGGATTGGTGGAAGCTATCATTCTTGAAGTGAAGGACAGGGCAAGGATTGGAGCTTCTTTCGCCCAATCGGATCAAATATGCTGCACCAGGAATCTTTCGAATTCTGGATACTGCACGGTGGGAGAGGTTGTTATCCACAAAAATCAGGACAACCCGGAGTGGCCTGTGCGCATCAAAACATTCTTTAATGGAAAAGATGAAGAGGCGAAGATGGAAATTAAGACGGTTGATATCAACAGTTCCGGAATGTACTACCTTTATTTCATGTACTGTGATCCGCAACTCAAGGGTACGCTGATTAAAGGAACGACCATCTGGAGAAACCCAGATGGTTATTTGCCTGGGAAGATGACTCCTTTGATGACATTCTATGGCTTTATGTCTTTAGCTTACCTTGTGCTTGGACTTGCCTGGTTTCTGAGGTTTGTTCAGTTTTGGAAGGATATTATACAATTGCACTACCATATCACAGCGGTGATTGCTCTTGGAATGTGCGAAATGGCTGTGTGGTACTTTGAGTATGCCAATTTTAATTCAACTGGGATCAGACCTATGGGCATTACATTGTGGGCTGTAACTTTTAGTGCTGTCAAGAAGACTCTTTCCCGCCTTCTTCTTTTGGTCGTTTCCATGGGTTTTGGTGTAGTGAAGCCTACACTTGGTGGTATAACTCCGAAAGTATTTCTTCTTGGTCTGGTATATTTCGTGGCATCCGAAGCACTTGAGCTTGTTGAACATTTGGGGAACATCAATGATTTTTCAGGAAAAACAAAGTTATTTTTGGTTCTACCTGTTGCCTTCTTAGATTCGTGGTTTATTCTATGGATTTTCTCATCGTTATCAAAAACTTTAGAGAAACTTCAGATCAGGAGAAACTTGGCTAAATTGGAGCTATACCGAAAATTTACCAACTCACTTGCAATATTTGTGCTTCTTTCGGTTGCTTGGATTGGGTTTGAGCTATACTTCAATGCAACGGATCCCCTGAGTGAGTTTTGGCAAATTGCTTGGATTATTCCAGCATTCTGGAGTATTCTTGCCTATGCTCTCTTGGCAATAATATGCATCCTTTGGGCCCCTTCACGTAACCCAACTAGATATGCATACATGGAGGAAGCAGGAGATGATTTTGATGAGGAAGGCATCTCGTTAACCAGTGGAGCGTTGAAGGTCAGTGGAGATGTGGCAATCAAGCGAGAATACGAGAATGCGCATGCAGATGATATGGAGGAGGATAAGCGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0008150 GO:0016192 GO:0016197 GO:0016482 GO:0042147 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lung_7-TM_R

Protein Analysis

568

Amino Acids

64.54

Weight (kDa)

5.91

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 271 - 516 5.5e-93 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 418
Acc16I TGCGCA 2 cut(s) 626, 1668
AccII CGCG 1 cut(s) 96
AccIII TCCGGA 1 cut(s) 698
AciI CCGC 6 cut(s) 39, 94, 294, 734, 929, 1063
AclWI GGATC 5 cut(s) 534, 725, 1007, 1430, 1443
AcsI RAATTY 2 cut(s) 556, 1358
AcuI CTGAAG 2 cut(s) 330, 1301
AcyI GRCGYC 2 cut(s) 14, 42
AdeI CACNNNGTG 1 cut(s) 1118
AfaI GTAC 5 cut(s) 245, 707, 725, 748, 968
AfiI CCNNNNNNNGG 2 cut(s) 367, 1115
AgsI TTSAA 7 cut(s) 234, 262, 482, 993, 1190, 1426, 1624
AjnI CCWGG 3 cut(s) 542, 796, 866
AleI CACNNNNGTG 2 cut(s) 404, 929
AluBI AGCT 7 cut(s) 257, 470, 509, 845, 1183, 1348, 1417
AluI AGCT 7 cut(s) 257, 470, 509, 845, 1183, 1348, 1417
AlwI GGATC 5 cut(s) 534, 725, 1007, 1430, 1443
Ama87I CYCGRG 1 cut(s) 107
Aor13HI TCCGGA 1 cut(s) 698
AoxI GGCC 4 cut(s) 5, 304, 617, 1530
ApaI GGGCCC 1 cut(s) 1534
ApeKI GCWGC 2 cut(s) 445, 537
ApoI RAATTY 2 cut(s) 556, 1358
AspLEI GCGC 3 cut(s) 98, 627, 1669
AspS9I GGNCC 3 cut(s) 322, 1530, 1531
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 3 cut(s) 73, 134, 943
AsuII TTCGAA 1 cut(s) 554
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 1534
BamHI GGATCC 1 cut(s) 1435
BanII GRGCYC 2 cut(s) 167, 1534
BauI CACGAG 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 1058
BbvI GCAGC 2 cut(s) 457, 524
BccI CCATC 4 cut(s) 92, 661, 776, 779
BciT130I CCWGG 3 cut(s) 544, 798, 868
BciVI GTATCC 1 cut(s) 557
BcnI CCSGG 1 cut(s) 611
BfaI CTAG 1 cut(s) 1551
BfuI GTATCC 1 cut(s) 557
BisI GCNGC 4 cut(s) 40, 295, 446, 538
BlsI GCNGC 4 cut(s) 41, 296, 447, 539
Bme1390I CCNGG 4 cut(s) 544, 611, 798, 868
Bme18I GGWCC 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 3 cut(s) 322, 1530, 1531
BmiI GGNNCC 3 cut(s) 1437, 1532, 1533
BmrFI CCNGG 4 cut(s) 544, 611, 798, 868
BmrI ACTGGG 1 cut(s) 1005
BmsI GCATC 6 cut(s) 217, 222, 636, 1175, 1530, 1608
BmuI ACTGGG 1 cut(s) 1005
BpiI GAAGAC 1 cut(s) 1058
BpmI CTGGAG 2 cut(s) 793, 1504
Bpu14I TTCGAA 1 cut(s) 554
BpuEI CTTGAG 3 cut(s) 350, 725, 1199
BpuMI CCSGG 1 cut(s) 611
BsaAI YACGTR 1 cut(s) 1541
BsaHI GRCGYC 2 cut(s) 14, 42
BsaJI CCNNGG 4 cut(s) 289, 318, 797, 1086
BsaWI WCCGGW 1 cut(s) 698
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 367, 1115
Bse1I ACTGG 3 cut(s) 460, 1000, 1611
BseAI TCCGGA 1 cut(s) 698
BseBI CCWGG 3 cut(s) 544, 798, 868
BseDI CCNNGG 4 cut(s) 289, 318, 797, 1086
BseGI GGATG 2 cut(s) 1166, 1521
BseLI CCNNNNNNNGG 2 cut(s) 367, 1115
BseMII CTCAG 4 cut(s) 90, 281, 866, 1433
BseNI ACTGG 3 cut(s) 460, 1000, 1611
BseRI GAGGAG 1 cut(s) 1700
BseSI GKGCMC 1 cut(s) 1534
BseXI GCAGC 2 cut(s) 457, 524
BsgI GTGCAG 2 cut(s) 523, 553
Bsh1236I CGCG 1 cut(s) 96
BshFI GGCC 4 cut(s) 7, 306, 619, 1532
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 2 cut(s) 611, 699
BslI CCNNNNNNNGG 2 cut(s) 367, 1115
BsmI GAATGC 2 cut(s) 1478, 1669
BsnI GGCC 4 cut(s) 7, 306, 619, 1532
BsoBI CYCGRG 1 cut(s) 107
Bsp119I TTCGAA 1 cut(s) 554
Bsp120I GGGCCC 1 cut(s) 1530
Bsp1286I GDGCHC 2 cut(s) 167, 1534
Bsp13I TCCGGA 1 cut(s) 698
Bsp143I GATC 5 cut(s) 526, 730, 999, 1320, 1435
Bsp19I CCATGG 2 cut(s) 289, 1086
BspACI CCGC 6 cut(s) 39, 94, 294, 734, 929, 1063
BspANI GGCC 4 cut(s) 7, 306, 619, 1532
BspCNI CTCAG 4 cut(s) 91, 280, 867, 1434
BspEI TCCGGA 1 cut(s) 698
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 3 cut(s) 1437, 1532, 1533
BspPI GGATC 5 cut(s) 534, 725, 1007, 1430, 1443
BspT104I TTCGAA 1 cut(s) 554
BsrI ACTGG 3 cut(s) 460, 1000, 1611
BssECI CCNNGG 4 cut(s) 289, 318, 797, 1086
BssMI GATC 5 cut(s) 526, 730, 999, 1320, 1435
BssNI GRCGYC 2 cut(s) 14, 42
BssSI CACGAG 1 cut(s) 239
BssT1I CCWWGG 3 cut(s) 289, 318, 1086
Bst2BI CACGAG 1 cut(s) 239
Bst2UI CCWGG 3 cut(s) 544, 798, 868
Bst4CI ACNGT 4 cut(s) 574, 682, 695, 728
Bst6I CTCTTC 1 cut(s) 651
BstACI GRCGYC 2 cut(s) 14, 42
BstAPI GCANNNNNTGC 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 1541
BstBI TTCGAA 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 167, 1671
BstDEI CTNAG 6 cut(s) 9, 99, 267, 875, 1258, 1442
BstDSI CCRYGG 2 cut(s) 289, 1086
BstF5I GGATG 2 cut(s) 1166, 1521
BstFNI CGCG 1 cut(s) 96
BstHHI GCGC 3 cut(s) 98, 627, 1669
BstKTI GATC 5 cut(s) 529, 733, 1002, 1323, 1438
BstMBI GATC 5 cut(s) 526, 730, 999, 1320, 1435
BstMWI GCNNNNNNNGC 7 cut(s) 13, 210, 303, 515, 935, 1172, 1508
BstNI CCWGG 3 cut(s) 544, 798, 868
BstNSI RCATGY 2 cut(s) 204, 1673
BstSCI CCNGG 4 cut(s) 542, 609, 796, 866
BstSLI GKGCMC 1 cut(s) 1534
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 457, 524
BstV2I GAAGAC 1 cut(s) 1058
BstX2I RGATCY 1 cut(s) 1435
BstXI CCANNNNNNTGG 1 cut(s) 1482
BstYI RGATCY 1 cut(s) 1435
BsuI GTATCC 1 cut(s) 557
BsuRI GGCC 4 cut(s) 7, 306, 619, 1532
BtgI CCRYGG 2 cut(s) 289, 1086
BtsCI GGATG 2 cut(s) 1166, 1521
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 4 cut(s) 304, 453, 1618, 1636
Cac8I GCNNGC 2 cut(s) 167, 1671
CfoI GCGC 3 cut(s) 98, 627, 1669
Cfr13I GGNCC 3 cut(s) 322, 1530, 1531
CseI GACGC 2 cut(s) 22, 31
Csp6I GTAC 5 cut(s) 244, 706, 724, 747, 967
CviAII CATG 6 cut(s) 201, 290, 721, 1087, 1564, 1670
CviQI GTAC 5 cut(s) 244, 706, 724, 747, 967
DdeI CTNAG 6 cut(s) 9, 99, 267, 875, 1258, 1442
DpnI GATC 5 cut(s) 528, 732, 1001, 1322, 1437
DpnII GATC 5 cut(s) 526, 730, 999, 1320, 1435
DraIII CACNNNGTG 1 cut(s) 1118
DrdI GACNNNNNNGTC 1 cut(s) 418
DseDI GACNNNNNNGTC 1 cut(s) 418
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
Eco130I CCWWGG 3 cut(s) 289, 318, 1086
Eco147I AGGCCT 2 cut(s) 7, 306
Eco24I GRGCYC 2 cut(s) 167, 1534
Eco32I GATATC 1 cut(s) 688
Eco47I GGWCC 1 cut(s) 322
Eco57I CTGAAG 2 cut(s) 330, 1301
Eco88I CYCGRG 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 1531
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 3 cut(s) 542, 796, 866
EcoRV GATATC 1 cut(s) 688
EcoT14I CCWWGG 3 cut(s) 289, 318, 1086
EcoT22I ATGCAT 2 cut(s) 1523, 1561
EcoT38I GRGCYC 2 cut(s) 167, 1534
ErhI CCWWGG 3 cut(s) 289, 318, 1086
FaeI CATG 6 cut(s) 204, 293, 724, 1090, 1567, 1673
FalI AAGNNNNNCTT 2 cut(s) 1125, 1157
FatI CATG 6 cut(s) 200, 289, 720, 1086, 1563, 1669
FauI CCCGC 1 cut(s) 1070
Fnu4HI GCNGC 4 cut(s) 40, 295, 446, 538
FokI GGATG 2 cut(s) 1153, 1508
FriOI GRGCYC 2 cut(s) 167, 1534
Fsp4HI GCNGC 4 cut(s) 40, 295, 446, 538
FspAI RTGCGCAY 2 cut(s) 626, 1668
FspBI CTAG 1 cut(s) 1551
FspI TGCGCA 2 cut(s) 626, 1668
GlaI GCGC 3 cut(s) 97, 626, 1668
GluI GCNGC 4 cut(s) 40, 295, 446, 538
GsuI CTGGAG 2 cut(s) 793, 1504
HaeIII GGCC 4 cut(s) 7, 306, 619, 1532
HapII CCGG 2 cut(s) 611, 699
HgaI GACGC 2 cut(s) 22, 31
HhaI GCGC 3 cut(s) 98, 627, 1669
Hin1I GRCGYC 2 cut(s) 14, 42
Hin1II CATG 6 cut(s) 204, 293, 724, 1090, 1567, 1673
Hin6I GCGC 3 cut(s) 96, 625, 1667
HinP1I GCGC 3 cut(s) 96, 625, 1667
HincII GTYRAC 1 cut(s) 1608
HindII GTYRAC 1 cut(s) 1608
HindIII AAGCTT 1 cut(s) 255
HinfI GANTC 7 cut(s) 103, 329, 419, 547, 808, 1054, 1262
HpaI GTTAAC 1 cut(s) 1608
HpaII CCGG 2 cut(s) 611, 699
HphI GGTGA 3 cut(s) 73, 134, 943
Hpy166II GTNNAC 2 cut(s) 325, 1608
Hpy188I TCNGA 7 cut(s) 226, 526, 876, 1004, 1131, 1171, 1320
Hpy8I GTNNAC 2 cut(s) 325, 1608
Hpy99I CGWCG 1 cut(s) 47
HpyCH4III ACNGT 4 cut(s) 574, 682, 695, 728
HpyCH4IV ACGT 1 cut(s) 1540
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 210, 303, 515, 935, 1172, 1508
HpyF3I CTNAG 6 cut(s) 9, 99, 267, 875, 1258, 1442
HpySE526I ACGT 1 cut(s) 1540
Hsp92I GRCGYC 2 cut(s) 14, 42
Hsp92II CATG 6 cut(s) 204, 293, 724, 1090, 1567, 1673
HspAI GCGC 3 cut(s) 96, 625, 1667
Kpn2I TCCGGA 1 cut(s) 698
KspAI GTTAAC 1 cut(s) 1608
Kzo9I GATC 5 cut(s) 526, 730, 999, 1320, 1435
LmnI GCTCC 3 cut(s) 506, 1345, 1616
Lsp1109I GCAGC 2 cut(s) 457, 524
LweI GCATC 6 cut(s) 217, 222, 636, 1175, 1530, 1608
MaeI CTAG 1 cut(s) 1551
MaeII ACGT 1 cut(s) 1540
MaeIII GTNAC 3 cut(s) 397, 1030, 1541
MalI GATC 5 cut(s) 528, 732, 1001, 1322, 1437
MboI GATC 5 cut(s) 526, 730, 999, 1320, 1435
MfeI CAATTG 1 cut(s) 908
MflI RGATCY 1 cut(s) 1435
MhlI GDGCHC 2 cut(s) 167, 1534
MluCI AATT 9 cut(s) 180, 556, 672, 908, 982, 988, 1340, 1358, 1458
MlyI GAGTC 3 cut(s) 428, 802, 1048
Mph1103I ATGCAT 2 cut(s) 1523, 1561
MroI TCCGGA 1 cut(s) 698
MseI TTAA 6 cut(s) 179, 642, 675, 756, 987, 1607
MslI CAYNNNNRTG 3 cut(s) 404, 929, 1674
MspA1I CMGCKG 1 cut(s) 929
MspI CCGG 2 cut(s) 611, 699
MspR9I CCNGG 4 cut(s) 544, 611, 798, 868
MunI CAATTG 1 cut(s) 908
Mva1269I GAATGC 2 cut(s) 1478, 1669
MvaI CCWGG 3 cut(s) 544, 798, 868
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 7 cut(s) 13, 210, 303, 515, 935, 1172, 1508
NciI CCSGG 1 cut(s) 611
NcoI CCATGG 2 cut(s) 289, 1086
NdeII GATC 5 cut(s) 526, 730, 999, 1320, 1435
NlaIII CATG 6 cut(s) 204, 293, 724, 1090, 1567, 1673
NlaIV GGNNCC 3 cut(s) 1437, 1532, 1533
NmuCI GTSAC 1 cut(s) 397
NsbI TGCGCA 2 cut(s) 626, 1668
NsiI ATGCAT 2 cut(s) 1523, 1561
NspI RCATGY 2 cut(s) 204, 1673
NspV TTCGAA 1 cut(s) 554
OliI CACNNNNGTG 2 cut(s) 404, 929
PaeI GCATGC 1 cut(s) 1673
PaeR7I CTCGAG 1 cut(s) 107
PceI AGGCCT 2 cut(s) 7, 306
PcsI WCGNNNNNNNCGW 2 cut(s) 333, 1167
PctI GAATGC 2 cut(s) 1478, 1669
PfeI GAWTC 4 cut(s) 103, 329, 547, 1262
PkrI GCNGC 4 cut(s) 41, 296, 447, 539
PleI GAGTC 3 cut(s) 427, 802, 1048
PpsI GAGTC 3 cut(s) 427, 802, 1048
Ppu21I YACGTR 1 cut(s) 1541
Psp6I CCWGG 3 cut(s) 542, 796, 866
PspGI CCWGG 3 cut(s) 542, 796, 866
PspN4I GGNNCC 3 cut(s) 1437, 1532, 1533
PspOMI GGGCCC 1 cut(s) 1530
PspPI GGNCC 3 cut(s) 322, 1530, 1531
PsuI RGATCY 1 cut(s) 1435
RsaI GTAC 5 cut(s) 245, 707, 725, 748, 968
RsaNI GTAC 5 cut(s) 244, 706, 724, 747, 967
RseI CAYNNNNRTG 3 cut(s) 404, 929, 1674
SaqAI TTAA 6 cut(s) 179, 642, 675, 756, 987, 1607
SatI GCNGC 4 cut(s) 40, 295, 446, 538
Sau3AI GATC 5 cut(s) 526, 730, 999, 1320, 1435
Sau96I GGNCC 3 cut(s) 322, 1530, 1531
SchI GAGTC 3 cut(s) 428, 802, 1048
ScrFI CCNGG 4 cut(s) 544, 611, 798, 868
SduI GDGCHC 2 cut(s) 167, 1534
SfaNI GCATC 6 cut(s) 217, 222, 636, 1175, 1530, 1608
Sfr274I CTCGAG 1 cut(s) 107
SfuI TTCGAA 1 cut(s) 554
SinI GGWCC 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 107
SmiMI CAYNNNNRTG 3 cut(s) 404, 929, 1674
SmlI CTYRAG 4 cut(s) 107, 365, 740, 1178
SmoI CTYRAG 4 cut(s) 107, 365, 740, 1178
SphI GCATGC 1 cut(s) 1673
Sse9I AATT 9 cut(s) 180, 556, 672, 908, 982, 988, 1340, 1358, 1458
SseBI AGGCCT 2 cut(s) 7, 306
SsiI CCGC 6 cut(s) 39, 94, 294, 734, 929, 1063
SspI AATATT 1 cut(s) 1380
SspMI CTAG 1 cut(s) 1551
StuI AGGCCT 2 cut(s) 7, 306
StyD4I CCNGG 4 cut(s) 542, 609, 796, 866
StyI CCWWGG 3 cut(s) 289, 318, 1086
TaaI ACNGT 4 cut(s) 574, 682, 695, 728
TaiI ACGT 1 cut(s) 1543
TaqI TCGA 2 cut(s) 108, 554
TasI AATT 9 cut(s) 180, 556, 672, 908, 982, 988, 1340, 1358, 1458
TatI WGTACW 3 cut(s) 243, 705, 723
TauI GCSGC 2 cut(s) 42, 297
TfiI GAWTC 4 cut(s) 103, 329, 547, 1262
Tru1I TTAA 6 cut(s) 179, 642, 675, 756, 987, 1607
Tru9I TTAA 6 cut(s) 179, 642, 675, 756, 987, 1607
TscAI CASTG 4 cut(s) 304, 460, 1618, 1636
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 2 cut(s) 445, 537
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 4 cut(s) 267, 370, 669, 709
TspGWI ACGGA 1 cut(s) 1448
TspRI CASTG 4 cut(s) 304, 460, 1618, 1636
VpaK11BI GGWCC 1 cut(s) 322
XapI RAATTY 2 cut(s) 556, 1358
XceI RCATGY 2 cut(s) 204, 1673
XhoI CTCGAG 1 cut(s) 107
XspI CTAG 1 cut(s) 1551
Zsp2I ATGCAT 2 cut(s) 1523, 1561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.