Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0001656

Physical Location & Seq

Genomic Coordinates Forward (+)

23173069 .. 23177663

Gene Structure 4595 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1380 bp

ATGGCGGCTGGTGATGGTGGTGGTCAGGTGGTGAAAGTCAAGAGGGCGAAACTGGAGGCATGCATGACGTGCCCTCTTTGTAATAAGCTGTTGAAGGAGGCTACAACTATTTCACTCTGCCTCCACACGTTTTGCAGGACGTGCATTTACGAGAAGTTATCAGATGAGGAGGGGGACTGCTGTCCCGTCTGTGAAATTGATCTCGGAATTTTACCTGTCGAAAAACTCAGGCCAGACCATAATTTGCACGATATTATAGCCAAGATATTTCCTGTTAAAGGAAAGAAAGTTGATGCACCTGAAGCTAACAAGCCTGAAGCTAATGATCCTGATGTTACGCCATCTTCGTTGCCAGTGAAAAGGAAGGAGAGATCATTATCTTCATTGGTAGTCAGTGCCCCCAAAGTTCCAGTGCAGTCAGGTATGACTGGAAAGAGATCAAGAGCTTTGTCAAGAAAGGGTGCTGCTTTACGAGGATGCAGTTTTTCTGCTGAAGAACCTGCGAAGAAGGATGATTCTACAGAAGACCATCCAGTGAGCTCTAACTCAACTGGTAATCTTGTTGAATTCGCACGAAATAAGAGCCAGGATTTGCTTGAGCATTCATATGAGCGAAATCCTAACAAAGATATGAACAATGACGTTGAAATAACAGAAGGGAAAGCTGACCTGTGGACTCCCTTAAACTGTCTTGTAGAGGCTGCTAACCGAACCAAGTCCTCGAAGCCAAATTCACAAGGGTTGCATGCCACGTCAGATCCAATCAATTCCCTTGATAATGAACTTTATATGTCTCAAAATAAGGCAGAAGGAGAATCGCCAAATGCTCTGGACTGCATCAGGAATGCATATACGCCCAAAACCAAAAAAAAGGAGGCCAACCAGAATGCAAGAGGAAAAAATAATAGTAATGGAGGAGGGGCATCAGGACCGGTGAAACGTAAAAGGGTGCGTGCAGCTAATCAGAAGAGGGTAGCAGCATCTGGGGAAGCAAGTGCCTCAACTCAACCTATGCTAGATGTATGTGGGGTTAAGTATAGAAATGTTCCCGTTTGGTTCTCCTTGGTAGCATCTGAAGACTGGAAAGGAGAAATTTCATTGCCACAGATATCAGCATGTTGCTTGAAAATAAAGGATGGTAAAATGCCTGTATCAATAATCCAGAAGTACCTTGCGATGAAACTTGAACTTAGCAGTGAAAATGAGGTGGAGATACTGTGTCGGGGTCAGCCAGTGCTTCCAACATGGCATCTAGAAACCCTAGTTGAGATGTGGTGCCGAACAGCACCAACAACGAAAAGGGTACCGGCAACAGTTGGCTCCTCGGCTAAAGATTTTGTAATGGTCCTCAATTATTGTCGTAGGGTTCCAAATCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050896 GO:0051865 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_2

Protein Analysis

460

Amino Acids

50.11

Weight (kDa)

8.81

Isoelectric Point (pI)

50.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_2	PF13923	24 - 64	3.3e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	24 - 64	1.4e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001656)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06770 AT1G06770 AT1G06770 AT2G30580 AT2G30580 AT2G30580
fragaria_vesca	FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22400 FvH4_6g02710 FvH4_6g02710
malus_domestica	MD04G1225500.v1.1 MD05G1204700.v1.1 MD10G1191800.v1.1 MD12G1242500.v1.1
prunus_persica	Prupe.6G344100_v2.0.a1 Prupe.6G344100_v2.0.a1 Prupe.8G231300_v2.0.a1
pyrus_communis	pycom04g19880 pycom05g19090 pycom10g16540 pycom12g22160
rosa_chinensis	RchiOBHm_Chr3g0450721 RchiOBHm_Chr6g0289351
rosa_laevigata	RLG00000012310 RLG00000025707
rosa_multiflora	Rmu_sc0000078.1_g000029 Rmu_sc0041561.1_g000001
rosa_roxburghii	Rroxscaffold_6G00428390 Rroxscaffold_7G00179330
rosa_rugosa	Rorug02G0629900 Rorug02G0630000 Rorug06G0202900
rosa_samantha	Rh3AG030800 Rh3BG031300 Rh3CG030100 Rh3DG031000 Rh6AG313800 Rh6BG321300 Rh6CG327600 Rh6DG313200
rosa_wichuraiana	Rw3G002380 Rw6G027130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	508
Acc65I	GGTACC	1 cut(s)	1303
AccB1I	GGYRCC	2 cut(s)	1275, 1303
AciI	CCGC	1 cut(s)	5
AclWI	GGATC	2 cut(s)	320, 752
AcsI	RAATTY	4 cut(s)	207, 566, 730, 1092
AcuI	CTGAAG	4 cut(s)	321, 336, 513, 1095
AfaI	GTAC	2 cut(s)	1169, 1305
AfiI	CCNNNNNNNGG	1 cut(s)	278
AflIII	ACRYGT	1 cut(s)	126
AgeI	ACCGGT	1 cut(s)	931
AgsI	TTSAA	5 cut(s)	94, 566, 647, 1126, 1187
AjiI	CACGTC	3 cut(s)	69, 141, 753
AjnI	CCWGG	1 cut(s)	585
AluBI	AGCT	7 cut(s)	88, 305, 320, 446, 540, 665, 959
AluI	AGCT	7 cut(s)	88, 305, 320, 446, 540, 665, 959
Alw21I	GWGCWC	1 cut(s)	542
Alw26I	GTCTC	1 cut(s)	798
AlwI	GGATC	2 cut(s)	320, 752
AlwNI	CAGNNNCTG	1 cut(s)	983
AoxI	GGCC	2 cut(s)	230, 876
ApeKI	GCWGC	4 cut(s)	464, 701, 956, 977
ApoI	RAATTY	4 cut(s)	207, 566, 730, 1092
AsiGI	ACCGGT	1 cut(s)	931
Asp718I	GGTACC	1 cut(s)	1303
AspS9I	GGNCC	2 cut(s)	929, 1345
AsuHPI	GGTGA	3 cut(s)	23, 43, 946
AvaII	GGWCC	2 cut(s)	929, 1345
BaeGI	GKGCMC	2 cut(s)	74, 400
BanI	GGYRCC	2 cut(s)	1275, 1303
BanII	GRGCYC	1 cut(s)	542
BbsI	GAAGAC	2 cut(s)	531, 1083
Bbv12I	GWGCWC	1 cut(s)	542
BbvI	GCAGC	4 cut(s)	451, 688, 968, 989
BccI	CCATC	4 cut(s)	8, 349, 537, 1130
BciT130I	CCWGG	1 cut(s)	587
BcoDI	GTCTC	1 cut(s)	798
BfaI	CTAG	3 cut(s)	1016, 1253, 1262
BfmI	CTRYAG	1 cut(s)	519
BfuAI	ACCTGC	1 cut(s)	508
BisI	GCNGC	5 cut(s)	6, 465, 702, 957, 978
BlsI	GCNGC	5 cut(s)	7, 466, 703, 958, 979
Bme1390I	CCNGG	1 cut(s)	587
Bme18I	GGWCC	2 cut(s)	929, 1345
BmgBI	CACGTC	3 cut(s)	69, 141, 753
BmgT120I	GGNCC	2 cut(s)	929, 1345
BmiI	GGNNCC	4 cut(s)	1277, 1305, 1321, 1368
BmrFI	CCNGG	1 cut(s)	587
BmsI	GCATC	7 cut(s)	283, 467, 846, 932, 989, 1079, 1258
BoxI	GACNNNNGTC	1 cut(s)	180
BpiI	GAAGAC	2 cut(s)	531, 1083
BpmI	CTGGAG	1 cut(s)	74
BpuEI	CTTGAG	1 cut(s)	617
BsaBI	GATNNNNATC	1 cut(s)	376
BsaJI	CCNNGG	2 cut(s)	1062, 1323
BsaWI	WCCGGW	1 cut(s)	931
BsaXI	ACNNNNNCTCC	4 cut(s)	105, 135, 1202, 1232
Bsc4I	CCNNNNNNNGG	1 cut(s)	278
Bse118I	RCCGGY	2 cut(s)	931, 1306
Bse1I	ACTGG	8 cut(s)	57, 353, 410, 433, 533, 556, 1085, 1232
Bse3DI	GCAATG	1 cut(s)	1097
Bse8I	GATNNNNATC	1 cut(s)	376
BseBI	CCWGG	1 cut(s)	587
BseDI	CCNNGG	2 cut(s)	1062, 1323
BseGI	GGATG	4 cut(s)	482, 517, 529, 1141
BseJI	GATNNNNATC	1 cut(s)	376
BseLI	CCNNNNNNNGG	1 cut(s)	278
BseMI	GCAATG	1 cut(s)	1097
BseMII	CTCAG	1 cut(s)	241
BseNI	ACTGG	8 cut(s)	57, 353, 410, 433, 533, 556, 1085, 1232
BseRI	GAGGAG	3 cut(s)	182, 930, 1312
BseSI	GKGCMC	2 cut(s)	74, 400
BseXI	GCAGC	4 cut(s)	451, 688, 968, 989
BsgI	GTGCAG	2 cut(s)	434, 975
BshFI	GGCC	2 cut(s)	232, 878
BshNI	GGYRCC	2 cut(s)	1275, 1303
BshTI	ACCGGT	1 cut(s)	931
BsiHKAI	GWGCWC	1 cut(s)	542
BsiSI	CCGG	2 cut(s)	932, 1307
BslFI	GGGAC	2 cut(s)	168, 188
BslI	CCNNNNNNNGG	1 cut(s)	278
BsmAI	GTCTC	1 cut(s)	798
BsmFI	GGGAC	2 cut(s)	168, 188
BsmI	GAATGC	3 cut(s)	601, 850, 892
BsnI	GGCC	2 cut(s)	232, 878
Bsp1286I	GDGCHC	3 cut(s)	74, 400, 542
Bsp143I	GATC	5 cut(s)	199, 325, 371, 437, 757
BspACI	CCGC	1 cut(s)	5
BspANI	GGCC	2 cut(s)	232, 878
BspCNI	CTCAG	1 cut(s)	240
BspLI	GGNNCC	4 cut(s)	1277, 1305, 1321, 1368
BspMI	ACCTGC	1 cut(s)	508
BspPI	GGATC	2 cut(s)	320, 752
BspT107I	GGYRCC	2 cut(s)	1275, 1303
BsrDI	GCAATG	1 cut(s)	1097
BsrFI	RCCGGY	2 cut(s)	931, 1306
BsrI	ACTGG	8 cut(s)	57, 353, 410, 433, 533, 556, 1085, 1232
BssAI	RCCGGY	2 cut(s)	931, 1306
BssECI	CCNNGG	2 cut(s)	1062, 1323
BssMI	GATC	5 cut(s)	199, 325, 371, 437, 757
BssT1I	CCWWGG	1 cut(s)	1062
Bst2UI	CCWGG	1 cut(s)	587
Bst4CI	ACNGT	3 cut(s)	689, 1218, 1315
Bst6I	CTCTTC	1 cut(s)	962
BstAPI	GCANNNNNTGC	2 cut(s)	69, 141
BstC8I	GCNNGC	3 cut(s)	61, 747, 954
BstDEI	CTNAG	2 cut(s)	227, 1190
BstENI	CCTNNNNNAGG	1 cut(s)	276
BstF5I	GGATG	4 cut(s)	482, 517, 529, 1141
BstKTI	GATC	5 cut(s)	202, 328, 374, 440, 760
BstMAI	GTCTC	1 cut(s)	798
BstMBI	GATC	5 cut(s)	199, 325, 371, 437, 757
BstMWI	GCNNNNNNNGC	3 cut(s)	69, 141, 302
BstNI	CCWGG	1 cut(s)	587
BstNSI	RCATGY	3 cut(s)	63, 749, 1119
BstPAI	GACNNNNGTC	1 cut(s)	180
BstSCI	CCNGG	1 cut(s)	585
BstSFI	CTRYAG	1 cut(s)	519
BstSLI	GKGCMC	2 cut(s)	74, 400
BstV1I	GCAGC	4 cut(s)	451, 688, 968, 989
BstV2I	GAAGAC	2 cut(s)	531, 1083
BstX2I	RGATCY	1 cut(s)	757
BstYI	RGATCY	1 cut(s)	757
BsuRI	GGCC	2 cut(s)	232, 878
BtgZI	GCGATG	1 cut(s)	1190
BtrI	CACGTC	3 cut(s)	69, 141, 753
BtsCI	GGATG	4 cut(s)	482, 517, 529, 1141
BtsI	GCAGTG	1 cut(s)	1201
BtsIMutI	CAGTG	6 cut(s)	360, 400, 417, 540, 1201, 1239
BveI	ACCTGC	1 cut(s)	508
Cac8I	GCNNGC	3 cut(s)	61, 747, 954
CaiI	CAGNNNCTG	1 cut(s)	983
Cfr10I	RCCGGY	2 cut(s)	931, 1306
Cfr13I	GGNCC	2 cut(s)	929, 1345
Csp6I	GTAC	2 cut(s)	1168, 1304
CspAI	ACCGGT	1 cut(s)	931
CspCI	CAANNNNNGTGG	2 cut(s)	113, 148
CviAII	CATG	5 cut(s)	60, 64, 746, 1116, 1245
CviQI	GTAC	2 cut(s)	1168, 1304
DdeI	CTNAG	2 cut(s)	227, 1190
DpnI	GATC	5 cut(s)	201, 327, 373, 439, 759
DpnII	GATC	5 cut(s)	199, 325, 371, 437, 757
Eam1104I	CTCTTC	1 cut(s)	962
EarI	CTCTTC	1 cut(s)	962
Ecl136II	GAGCTC	1 cut(s)	540
Eco130I	CCWWGG	1 cut(s)	1062
Eco24I	GRGCYC	1 cut(s)	542
Eco32I	GATATC	1 cut(s)	1110
Eco47I	GGWCC	2 cut(s)	929, 1345
Eco53kI	GAGCTC	1 cut(s)	540
Eco57I	CTGAAG	4 cut(s)	321, 336, 513, 1095
EcoICRI	GAGCTC	1 cut(s)	540
EcoNI	CCTNNNNNAGG	1 cut(s)	276
EcoRI	GAATTC	1 cut(s)	566
EcoRII	CCWGG	1 cut(s)	585
EcoRV	GATATC	1 cut(s)	1110
EcoT14I	CCWWGG	1 cut(s)	1062
EcoT22I	ATGCAT	2 cut(s)	65, 850
EcoT38I	GRGCYC	1 cut(s)	542
ErhI	CCWWGG	1 cut(s)	1062
FaeI	CATG	5 cut(s)	63, 67, 749, 1119, 1248
FaqI	GGGAC	2 cut(s)	168, 188
FatI	CATG	5 cut(s)	59, 63, 745, 1115, 1244
FauNDI	CATATG	1 cut(s)	607
Fnu4HI	GCNGC	5 cut(s)	6, 465, 702, 957, 978
FokI	GGATG	4 cut(s)	489, 516, 524, 1148
FriOI	GRGCYC	1 cut(s)	542
Fsp4HI	GCNGC	5 cut(s)	6, 465, 702, 957, 978
FspBI	CTAG	3 cut(s)	1016, 1253, 1262
GluI	GCNGC	5 cut(s)	6, 465, 702, 957, 978
GsuI	CTGGAG	1 cut(s)	74
HaeIII	GGCC	2 cut(s)	232, 878
HapII	CCGG	2 cut(s)	932, 1307
Hin1II	CATG	5 cut(s)	63, 67, 749, 1119, 1248
HinfI	GANTC	3 cut(s)	515, 676, 815
HpaII	CCGG	2 cut(s)	932, 1307
HphI	GGTGA	3 cut(s)	23, 43, 946
Hpy166II	GTNNAC	1 cut(s)	675
Hpy188I	TCNGA	5 cut(s)	163, 206, 757, 966, 1075
Hpy188III	TCNNGA	9 cut(s)	40, 329, 441, 453, 830, 841, 927, 1162, 1253
Hpy8I	GTNNAC	1 cut(s)	675
HpyAV	CCTTC	5 cut(s)	88, 358, 502, 650, 803
HpyCH4III	ACNGT	3 cut(s)	689, 1218, 1315
HpyCH4IV	ACGT	6 cut(s)	68, 128, 140, 642, 752, 940
HpyF10VI	GCNNNNNNNGC	3 cut(s)	69, 141, 302
HpyF3I	CTNAG	2 cut(s)	227, 1190
HpySE526I	ACGT	6 cut(s)	68, 128, 140, 642, 752, 940
Hsp92II	CATG	5 cut(s)	63, 67, 749, 1119, 1248
KpnI	GGTACC	1 cut(s)	1307
Kzo9I	GATC	5 cut(s)	199, 325, 371, 437, 757
LmnI	GCTCC	1 cut(s)	1325
Lsp1109I	GCAGC	4 cut(s)	451, 688, 968, 989
LweI	GCATC	7 cut(s)	283, 467, 846, 932, 989, 1079, 1258
MaeI	CTAG	3 cut(s)	1016, 1253, 1262
MaeII	ACGT	6 cut(s)	68, 128, 140, 642, 752, 940
MaeIII	GTNAC	1 cut(s)	334
MalI	GATC	5 cut(s)	201, 327, 373, 439, 759
MboI	GATC	5 cut(s)	199, 325, 371, 437, 757
MboII	GAAGA	7 cut(s)	336, 372, 506, 517, 536, 979, 1088
MflI	RGATCY	1 cut(s)	757
MhlI	GDGCHC	3 cut(s)	74, 400, 542
MluCI	AATT	8 cut(s)	195, 207, 241, 566, 730, 766, 1092, 1351
MlyI	GAGTC	1 cut(s)	670
MmeI	TCCRAC	1 cut(s)	1265
Mph1103I	ATGCAT	2 cut(s)	65, 850
MseI	TTAA	3 cut(s)	276, 683, 1032
MslI	CAYNNNNRTG	1 cut(s)	606
MspI	CCGG	2 cut(s)	932, 1307
MspR9I	CCNGG	1 cut(s)	587
Mva1269I	GAATGC	3 cut(s)	601, 850, 892
MvaI	CCWGG	1 cut(s)	587
MwoI	GCNNNNNNNGC	3 cut(s)	69, 141, 302
NdeI	CATATG	1 cut(s)	607
NdeII	GATC	5 cut(s)	199, 325, 371, 437, 757
NlaIII	CATG	5 cut(s)	63, 67, 749, 1119, 1248
NlaIV	GGNNCC	4 cut(s)	1277, 1305, 1321, 1368
NmeAIII	GCCGAG	1 cut(s)	1304
NsiI	ATGCAT	2 cut(s)	65, 850
NspI	RCATGY	3 cut(s)	63, 749, 1119
PaeI	GCATGC	2 cut(s)	63, 749
PcsI	WCGNNNNNNNCGW	1 cut(s)	344
PctI	GAATGC	3 cut(s)	601, 850, 892
PfeI	GAWTC	2 cut(s)	515, 815
PinAI	ACCGGT	1 cut(s)	931
PkrI	GCNGC	5 cut(s)	7, 466, 703, 958, 979
PleI	GAGTC	1 cut(s)	670
PpsI	GAGTC	1 cut(s)	670
PshAI	GACNNNNGTC	1 cut(s)	180
Psp124BI	GAGCTC	1 cut(s)	542
Psp6I	CCWGG	1 cut(s)	585
PspGI	CCWGG	1 cut(s)	585
PspN4I	GGNNCC	4 cut(s)	1277, 1305, 1321, 1368
PspPI	GGNCC	2 cut(s)	929, 1345
PstNI	CAGNNNCTG	1 cut(s)	983
PsuI	RGATCY	1 cut(s)	757
RsaI	GTAC	2 cut(s)	1169, 1305
RsaNI	GTAC	2 cut(s)	1168, 1304
RseI	CAYNNNNRTG	1 cut(s)	606
SacI	GAGCTC	1 cut(s)	542
SaqAI	TTAA	3 cut(s)	276, 683, 1032
SatI	GCNGC	5 cut(s)	6, 465, 702, 957, 978
Sau3AI	GATC	5 cut(s)	199, 325, 371, 437, 757
Sau96I	GGNCC	2 cut(s)	929, 1345
SchI	GAGTC	1 cut(s)	670
ScrFI	CCNGG	1 cut(s)	587
SduI	GDGCHC	3 cut(s)	74, 400, 542
SfaNI	GCATC	7 cut(s)	283, 467, 846, 932, 989, 1079, 1258
SfcI	CTRYAG	1 cut(s)	519
SinI	GGWCC	2 cut(s)	929, 1345
SmiMI	CAYNNNNRTG	1 cut(s)	606
SmlI	CTYRAG	1 cut(s)	596
SmoI	CTYRAG	1 cut(s)	596
SphI	GCATGC	2 cut(s)	63, 749
Sse9I	AATT	8 cut(s)	195, 207, 241, 566, 730, 766, 1092, 1351
SsiI	CCGC	1 cut(s)	5
SspMI	CTAG	3 cut(s)	1016, 1253, 1262
SstI	GAGCTC	1 cut(s)	542
StyD4I	CCNGG	1 cut(s)	585
StyI	CCWWGG	1 cut(s)	1062
TaaI	ACNGT	3 cut(s)	689, 1218, 1315
TaiI	ACGT	6 cut(s)	71, 131, 143, 645, 755, 943
TaqI	TCGA	2 cut(s)	219, 722
TasI	AATT	8 cut(s)	195, 207, 241, 566, 730, 766, 1092, 1351
TauI	GCSGC	1 cut(s)	8
TfiI	GAWTC	2 cut(s)	515, 815
Tru1I	TTAA	3 cut(s)	276, 683, 1032
Tru9I	TTAA	3 cut(s)	276, 683, 1032
TscAI	CASTG	6 cut(s)	360, 400, 417, 540, 1201, 1239
TseI	GCWGC	4 cut(s)	464, 701, 956, 977
TspDTI	ATGAA	6 cut(s)	372, 594, 647, 795, 1086, 1193
TspRI	CASTG	6 cut(s)	360, 400, 417, 540, 1201, 1239
VpaK11BI	GGWCC	2 cut(s)	929, 1345
XagI	CCTNNNNNAGG	1 cut(s)	276
XapI	RAATTY	4 cut(s)	207, 566, 730, 1092
XbaI	TCTAGA	1 cut(s)	1252
XceI	RCATGY	3 cut(s)	63, 749, 1119
XspI	CTAG	3 cut(s)	1016, 1253, 1262
Zsp2I	ATGCAT	2 cut(s)	65, 850

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.