pycom13g09690

Ankyrin repeats (3 copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002201
Physical Location & Seq
Forward (+)
6493329 .. 6495594
2266 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1806 bp
ATGCCACCATCTTACTTCCCTTTAAGGTGGGAAAGCACCGGAGACCAATGGTGGTATGCATCACCAATCGATTGGGCAGCAGCCAATGGCTTATATGATTTGGTCAGTGAGCTTCTTCACCTTGATACCAACCTCCTCATCAAGCTGACCTCATTGCGCCGCATCCACCGCCTTGAAACTGTCTGGGACGACGAGGCTCAGTTCGATGATGTTGCCAAATGCCGGTCACAAGTTGCCAAGAGGCTCCTCCACGAGTGCCAAATGCAGAGGGGACACAACTCTCTCATCAGAGCCGGCTACGGTGGATGGCTTCTCTACACTGCGGCCTCGGCTGGCGATATAGGGTTTGTCAGGGAGTTGCTGGGGAGAGACCCTCTTCTTGTTTTTGGAGAAGGAGAGTATGGAGTCACTGACATCTTCTATGCTGCTGCCAGGAGCAAGAACTCTGAGGTTTTCAGGCTGCTGCTTGATTTCTCAGTGTCTCCAAGGTTTAGCCTCAGTAATGGAGAACTTGAAGAGAGGTTGGGTGATGTTACATCGGATTTCAAGTGGGAAATGATGAACCGGGCGGTTCATGCCGCTGCTAGAGGTGGCAATTTGGAGATTTTGAGGGACCTGCTTGGGGATTGTGAGGATGTTTTGGCTTATAGAGATGCTCAGGGATCTACCATCTTGCATACAGCCTCTGGCAGAGGGCAGGTTGAGGTGGTTGAGTATATAATAGCATTCTTCGATACCATCACAATAGTAGATAATCAAGGGAACACAGCTCTACACGTGGCCGCTTATAGGGGTCACTTGGCTGTGGTGGATGTCCTAATTCGTGCATCTCCCTCGTTAACCACGTCATCAAACAATTATGGAGACACCTTTCTGCATTTGGCAGTGTCTGGTTTCAGAAGCCCTGGTTTCCGGCCACTGGACAAACAGATTGAGCTCATTAAGCAATTAGTATGTGGTGATATTGCGAACTTGCAGGACGTTGTTAATGTTAAGAACAATGATGGACGAACTGCTCTTCACATGGCTGTAATTGAGAATGTTCAATCTAATTTGGTGGAACTCCTCATGACTGTTCCATCAATTGATTTGAACATCCGTGACTCTGGCGGCATGACCCCTCTAGATATTCTTAAACGAAGGCCAAAGTCAGCTTCGTCCGAACTTCTGATCAAGGAATTGATTTCAGCTGGAGGGATCTCCAATCGTCAGGACCGTAAAGCAAGGAGCGCCCTTGTTTCCCATTTGAGAATGCAGGGTATTGGAGGCAGTCCGGGAACCTCTTTCAGAATTCCTGATGCAGAGATATTCTTATACACGGGCATTGACAATGCTTCTGATGCCAATTGCGATCAGTCAGGCGTGCAATTCAACACATTCTCAGGTGAGCTAAGACAGTTTGGCTCACCCAACACAGTAAACAATAAGAAGTCAAGTTCTGTAACTTATGCTGCAAGGCGCCTTAAGTTTCTTCTTCAATGGCGGAGGAGGAAAGAAAAAAAGGAATCTAGCATATACTTAAGAGATAATGATTCTGTGGAGTCATTTAGCACCTGCGTAGATTTGGACGACAATCCAATCCCTCTTCGGCAGATGTATGCCAAATCTTCCTCCCTCCCAAACAACAAAAGGACAGTTTCTGTAAGGAGTTTTCTTCCGAGCCCATACACTAAAATGAAATTTACTGCTGGTTTATCACACGGTGTGATTCAGGCAAGGCCTCATTTGGCTTTTCCAGCTGAGTCACTGTCTTCCCCTACTTTCATGGACAAAGAGAAGGGTGTTGACATTTTGGGATCGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5

Protein Analysis

602

Amino Acids

66.72

Weight (kDa)

6.52

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 192 - 275 2.2e-14 Ankyrin repeats (3 copies)
Ank_2 PF12796 249 - 297 5.4e-07 Ankyrin repeats (3 copies)
Ank_5 PF13857 251 - 295 2.3e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 310 - 377 7.6e-06 Ankyrin repeats (3 copies)
Ank_5 PF13857 329 - 377 2.9e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1562
Acc36I ACCTGC 3 cut(s) 624, 688, 1562
AccB1I GGYRCC 1 cut(s) 1458
AciI CCGC 8 cut(s) 160, 169, 323, 569, 579, 783, 1110, 1483
AclWI GGATC 3 cut(s) 670, 1205, 1804
AcoI YGGCCR 2 cut(s) 780, 914
AcsI RAATTY 2 cut(s) 1290, 1679
AcvI CACGTG 1 cut(s) 778
AcyI GRCGYC 1 cut(s) 1459
AdeI CACNNNGTG 2 cut(s) 1703, 1705
AfiI CCNNNNNNNGG 4 cut(s) 222, 622, 789, 919
AflII CTTAAG 2 cut(s) 1463, 1519
AflIII ACRYGT 1 cut(s) 775
AgsI TTSAA 7 cut(s) 176, 515, 547, 1046, 1093, 1372, 1478
AjiI CACGTC 1 cut(s) 846
AjnI CCWGG 2 cut(s) 431, 904
AjuI GAANNNNNNNTTGG 2 cut(s) 1138, 1170
AluBI AGCT 8 cut(s) 112, 145, 770, 937, 1154, 1190, 1390, 1739
AluI AGCT 8 cut(s) 112, 145, 770, 937, 1154, 1190, 1390, 1739
Alw21I GWGCWC 1 cut(s) 939
Alw26I GTCTC 4 cut(s) 36, 363, 486, 858
AlwI GGATC 3 cut(s) 670, 1205, 1804
AlwNI CAGNNNCTG 3 cut(s) 686, 890, 1640
AoxI GGCC 5 cut(s) 324, 780, 914, 1142, 1718
ApeKI GCWGC 8 cut(s) 77, 80, 425, 428, 460, 463, 581, 1451
ApoI RAATTY 2 cut(s) 1290, 1679
AspLEI GCGC 3 cut(s) 159, 1232, 1461
AspS9I GGNCC 2 cut(s) 613, 1213
AsuC2I CCSGG 2 cut(s) 566, 1275
AsuHPI GGTGA 6 cut(s) 54, 110, 539, 971, 1397, 1398
AvaII GGWCC 2 cut(s) 613, 1213
BanI GGYRCC 1 cut(s) 1458
BanII GRGCYC 2 cut(s) 939, 1664
BauI CACGAG 1 cut(s) 251
BbrPI CACGTG 1 cut(s) 778
BbsI GAAGAC 1 cut(s) 1743
Bbv12I GWGCWC 1 cut(s) 939
BbvI GCAGC 8 cut(s) 89, 92, 412, 415, 447, 450, 568, 1438
BccI CCATC 6 cut(s) 16, 300, 677, 746, 998, 1087
BcgI CGANNNNNNTGC 4 cut(s) 194, 228, 816, 850
BciT130I CCWGG 2 cut(s) 433, 906
BclI TGATCA 1 cut(s) 1170
BcnI CCSGG 2 cut(s) 566, 1275
BcoDI GTCTC 4 cut(s) 36, 363, 486, 858
BfaI CTAG 4 cut(s) 585, 1124, 1509, 1804
BfoI RGCGCY 2 cut(s) 1233, 1462
BfrI CTTAAG 2 cut(s) 1463, 1519
BfuAI ACCTGC 3 cut(s) 624, 688, 1562
Bme1390I CCNGG 4 cut(s) 433, 566, 906, 1275
Bme18I GGWCC 2 cut(s) 613, 1213
BmgBI CACGTC 1 cut(s) 846
BmgT120I GGNCC 2 cut(s) 613, 1213
BmiI GGNNCC 4 cut(s) 245, 614, 1279, 1460
BmrFI CCNGG 4 cut(s) 433, 566, 906, 1275
BmsI GCATC 6 cut(s) 68, 171, 643, 836, 1288, 1330
BpiI GAAGAC 1 cut(s) 1743
BplI GAGNNNNNCTC 2 cut(s) 358, 390
BpmI CTGGAG 1 cut(s) 1212
Bpu10I CCTNAGC 1 cut(s) 657
BpuMI CCSGG 2 cut(s) 566, 1275
Bsa29I ATCGAT 1 cut(s) 69
BsaAI YACGTR 1 cut(s) 778
BsaHI GRCGYC 1 cut(s) 1459
BsaI GGTCTC 2 cut(s) 36, 363
BsaJI CCNNGG 3 cut(s) 327, 485, 904
BsaWI WCCGGW 1 cut(s) 38
Bsc4I CCNNNNNNNGG 4 cut(s) 222, 622, 789, 919
Bse118I RCCGGY 2 cut(s) 222, 293
Bse1I ACTGG 1 cut(s) 924
Bse3DI GCAATG 1 cut(s) 152
BseBI CCWGG 2 cut(s) 433, 906
BseCI ATCGAT 1 cut(s) 69
BseDI CCNNGG 3 cut(s) 327, 485, 904
BseGI GGATG 5 cut(s) 162, 311, 640, 817, 1095
BseLI CCNNNNNNNGG 4 cut(s) 222, 622, 789, 919
BseMI GCAATG 1 cut(s) 152
BseMII CTCAG 7 cut(s) 212, 438, 489, 511, 671, 1395, 1731
BseNI ACTGG 1 cut(s) 924
BseRI GAGGAG 4 cut(s) 125, 236, 1055, 1501
BseXI GCAGC 8 cut(s) 89, 92, 412, 415, 447, 450, 568, 1438
BseYI CCCAGC 1 cut(s) 361
BshFI GGCC 5 cut(s) 326, 782, 916, 1144, 1720
BshNI GGYRCC 1 cut(s) 1458
BshVI ATCGAT 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 939
BsiSI CCGG 6 cut(s) 39, 223, 294, 565, 913, 1274
BslFI GGGAC 3 cut(s) 200, 285, 626
BslI CCNNNNNNNGG 4 cut(s) 222, 622, 789, 919
BsmAI GTCTC 4 cut(s) 36, 363, 486, 858
BsmFI GGGAC 3 cut(s) 200, 285, 626
BsmI GAATGC 2 cut(s) 725, 1257
BsnI GGCC 5 cut(s) 326, 782, 916, 1144, 1720
Bso31I GGTCTC 2 cut(s) 36, 363
Bsp1286I GDGCHC 2 cut(s) 939, 1664
Bsp143I GATC 5 cut(s) 662, 1170, 1197, 1351, 1796
BspACI CCGC 8 cut(s) 160, 169, 323, 569, 579, 783, 1110, 1483
BspANI GGCC 5 cut(s) 326, 782, 916, 1144, 1720
BspCNI CTCAG 7 cut(s) 211, 439, 488, 510, 670, 1394, 1732
BspDI ATCGAT 1 cut(s) 69
BspHI TCATGA 1 cut(s) 1068
BspLI GGNNCC 4 cut(s) 245, 614, 1279, 1460
BspMI ACCTGC 3 cut(s) 624, 688, 1562
BspPI GGATC 3 cut(s) 670, 1205, 1804
BspQI GCTCTTC 1 cut(s) 1023
BspT107I GGYRCC 1 cut(s) 1458
BspTI CTTAAG 2 cut(s) 1463, 1519
BspTNI GGTCTC 2 cut(s) 36, 363
BsrDI GCAATG 1 cut(s) 152
BsrFI RCCGGY 2 cut(s) 222, 293
BsrI ACTGG 1 cut(s) 924
BssAI RCCGGY 2 cut(s) 222, 293
BssECI CCNNGG 3 cut(s) 327, 485, 904
BssMI GATC 5 cut(s) 662, 1170, 1197, 1351, 1796
BssNI GRCGYC 1 cut(s) 1459
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 485
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 2 cut(s) 433, 906
Bst4CI ACNGT 9 cut(s) 181, 302, 1075, 1217, 1398, 1417, 1636, 1703, 1749
Bst6I CTCTTC 4 cut(s) 381, 510, 1023, 1590
BstACI GRCGYC 1 cut(s) 1459
BstAFI CTTAAG 2 cut(s) 1463, 1519
BstBAI YACGTR 1 cut(s) 778
BstC8I GCNNGC 3 cut(s) 295, 334, 1364
BstDEI CTNAG 8 cut(s) 198, 447, 475, 497, 657, 1381, 1391, 1740
BstF5I GGATG 5 cut(s) 162, 311, 640, 817, 1095
BstH2I RGCGCY 2 cut(s) 1233, 1462
BstHHI GCGC 3 cut(s) 159, 1232, 1461
BstKTI GATC 5 cut(s) 665, 1173, 1200, 1354, 1799
BstMAI GTCTC 4 cut(s) 36, 363, 486, 858
BstMBI GATC 5 cut(s) 662, 1170, 1197, 1351, 1796
BstMWI GCNNNNNNNGC 7 cut(s) 168, 329, 575, 943, 1229, 1340, 1736
BstNI CCWGG 2 cut(s) 433, 906
BstSCI CCNGG 4 cut(s) 431, 564, 904, 1273
BstV1I GCAGC 8 cut(s) 89, 92, 412, 415, 447, 450, 568, 1438
BstV2I GAAGAC 1 cut(s) 1743
BstX2I RGATCY 2 cut(s) 662, 1197
BstXI CCANNNNNNTGG 1 cut(s) 72
BstYI RGATCY 2 cut(s) 662, 1197
Bsu15I ATCGAT 1 cut(s) 69
BsuRI GGCC 5 cut(s) 326, 782, 916, 1144, 1720
BsuTUI ATCGAT 1 cut(s) 69
BtrI CACGTC 1 cut(s) 846
BtsCI GGATG 5 cut(s) 162, 311, 640, 817, 1095
BtsI GCAGTG 2 cut(s) 318, 891
BtsIMutI CAGTG 7 cut(s) 112, 318, 408, 483, 891, 917, 1745
BveI ACCTGC 3 cut(s) 624, 688, 1562
Cac8I GCNNGC 3 cut(s) 295, 334, 1364
CaiI CAGNNNCTG 3 cut(s) 686, 890, 1640
CciI TCATGA 1 cut(s) 1068
CfoI GCGC 3 cut(s) 159, 1232, 1461
Cfr10I RCCGGY 2 cut(s) 222, 293
Cfr13I GGNCC 2 cut(s) 613, 1213
ClaI ATCGAT 1 cut(s) 69
CviAII CATG 5 cut(s) 575, 1024, 1069, 1114, 1765
DdeI CTNAG 8 cut(s) 198, 447, 475, 497, 657, 1381, 1391, 1740
DinI GGCGCC 1 cut(s) 1460
DpnI GATC 5 cut(s) 664, 1172, 1199, 1353, 1798
DpnII GATC 5 cut(s) 662, 1170, 1197, 1351, 1796
DraIII CACNNNGTG 2 cut(s) 1703, 1705
EaeI YGGCCR 2 cut(s) 780, 914
Eam1104I CTCTTC 4 cut(s) 381, 510, 1023, 1590
EarI CTCTTC 4 cut(s) 381, 510, 1023, 1590
EciI GGCGGA 1 cut(s) 1498
Ecl136II GAGCTC 1 cut(s) 937
Eco130I CCWWGG 1 cut(s) 485
Eco147I AGGCCT 1 cut(s) 1720
Eco24I GRGCYC 2 cut(s) 939, 1664
Eco31I GGTCTC 2 cut(s) 36, 363
Eco47I GGWCC 2 cut(s) 613, 1213
Eco53kI GAGCTC 1 cut(s) 937
Eco72I CACGTG 1 cut(s) 778
EcoICRI GAGCTC 1 cut(s) 937
EcoO109I RGGNCCY 1 cut(s) 613
EcoRI GAATTC 1 cut(s) 1290
EcoRII CCWGG 2 cut(s) 431, 904
EcoT14I CCWWGG 1 cut(s) 485
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 2 cut(s) 939, 1664
EgeI GGCGCC 1 cut(s) 1460
EheI GGCGCC 1 cut(s) 1460
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 5 cut(s) 578, 1027, 1072, 1117, 1768
FaqI GGGAC 3 cut(s) 200, 285, 626
FatI CATG 5 cut(s) 574, 1023, 1068, 1113, 1764
FbaI TGATCA 1 cut(s) 1170
FokI GGATG 5 cut(s) 149, 318, 647, 824, 1082
FriOI GRGCYC 2 cut(s) 939, 1664
FspBI CTAG 4 cut(s) 585, 1124, 1509, 1804
GlaI GCGC 3 cut(s) 158, 1231, 1460
GsaI CCCAGC 1 cut(s) 365
GsuI CTGGAG 1 cut(s) 1212
HaeII RGCGCY 2 cut(s) 1233, 1462
HaeIII GGCC 5 cut(s) 326, 782, 916, 1144, 1720
HapII CCGG 6 cut(s) 39, 223, 294, 565, 913, 1274
HhaI GCGC 3 cut(s) 159, 1232, 1461
Hin1I GRCGYC 1 cut(s) 1459
Hin1II CATG 5 cut(s) 578, 1027, 1072, 1117, 1768
Hin6I GCGC 3 cut(s) 157, 1230, 1459
HinP1I GCGC 3 cut(s) 157, 1230, 1459
HincII GTYRAC 2 cut(s) 840, 1786
HindII GTYRAC 2 cut(s) 840, 1786
HinfI GANTC 7 cut(s) 405, 1103, 1505, 1532, 1541, 1708, 1742
HpaI GTTAAC 1 cut(s) 840
HpaII CCGG 6 cut(s) 39, 223, 294, 565, 913, 1274
HphI GGTGA 6 cut(s) 54, 110, 539, 971, 1397, 1398
Hpy166II GTNNAC 3 cut(s) 840, 1420, 1786
Hpy188I TCNGA 9 cut(s) 290, 448, 541, 899, 1162, 1170, 1289, 1339, 1659
Hpy188III TCNNGA 4 cut(s) 1069, 1124, 1211, 1295
Hpy8I GTNNAC 3 cut(s) 840, 1420, 1786
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 3 cut(s) 386, 1134, 1771
HpyCH4III ACNGT 9 cut(s) 181, 302, 1075, 1217, 1398, 1417, 1636, 1703, 1749
HpyCH4IV ACGT 3 cut(s) 777, 845, 981
HpyF10VI GCNNNNNNNGC 7 cut(s) 168, 329, 575, 943, 1229, 1340, 1736
HpyF3I CTNAG 8 cut(s) 198, 447, 475, 497, 657, 1381, 1391, 1740
HpySE526I ACGT 3 cut(s) 777, 845, 981
Hsp92I GRCGYC 1 cut(s) 1459
Hsp92II CATG 5 cut(s) 578, 1027, 1072, 1117, 1768
HspAI GCGC 3 cut(s) 157, 1230, 1459
KasI GGCGCC 1 cut(s) 1458
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 1170
KspAI GTTAAC 1 cut(s) 840
Kzo9I GATC 5 cut(s) 662, 1170, 1197, 1351, 1796
LguI GCTCTTC 1 cut(s) 1023
LmnI GCTCC 3 cut(s) 249, 435, 1227
Lsp1109I GCAGC 8 cut(s) 89, 92, 412, 415, 447, 450, 568, 1438
LweI GCATC 6 cut(s) 68, 171, 643, 836, 1288, 1330
MaeI CTAG 4 cut(s) 585, 1124, 1509, 1804
MaeII ACGT 3 cut(s) 777, 845, 981
MaeIII GTNAC 7 cut(s) 225, 406, 532, 794, 1100, 1441, 1743
MalI GATC 5 cut(s) 664, 1172, 1199, 1353, 1798
MboI GATC 5 cut(s) 662, 1170, 1197, 1351, 1796
MfeI CAATTG 2 cut(s) 1083, 1345
MflI RGATCY 2 cut(s) 662, 1197
MhlI GDGCHC 2 cut(s) 939, 1664
Mly113I GGCGCC 1 cut(s) 1459
MlyI GAGTC 4 cut(s) 414, 1097, 1550, 1751
Mph1103I ATGCAT 1 cut(s) 61
MroNI GCCGGC 1 cut(s) 293
MseI TTAA 8 cut(s) 23, 839, 942, 987, 993, 1134, 1464, 1520
MslI CAYNNNNRTG 1 cut(s) 1673
MspA1I CMGCKG 3 cut(s) 581, 1190, 1739
MspCI CTTAAG 2 cut(s) 1463, 1519
MspI CCGG 6 cut(s) 39, 223, 294, 565, 913, 1274
MspR9I CCNGG 4 cut(s) 433, 566, 906, 1275
MunI CAATTG 2 cut(s) 1083, 1345
Mva1269I GAATGC 2 cut(s) 725, 1257
MvaI CCWGG 2 cut(s) 433, 906
MwoI GCNNNNNNNGC 7 cut(s) 168, 329, 575, 943, 1229, 1340, 1736
NaeI GCCGGC 1 cut(s) 295
NarI GGCGCC 1 cut(s) 1459
NciI CCSGG 2 cut(s) 566, 1275
NdeII GATC 5 cut(s) 662, 1170, 1197, 1351, 1796
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 5 cut(s) 578, 1027, 1072, 1117, 1768
NlaIV GGNNCC 4 cut(s) 245, 614, 1279, 1460
NmeAIII GCCGAG 1 cut(s) 308
NmuCI GTSAC 5 cut(s) 225, 406, 794, 1100, 1743
NsiI ATGCAT 1 cut(s) 61
PagI TCATGA 1 cut(s) 1068
PaqCI CACCTGC 1 cut(s) 1562
PceI AGGCCT 1 cut(s) 1720
PciSI GCTCTTC 1 cut(s) 1023
PcsI WCGNNNNNNNCGW 2 cut(s) 842, 1213
PctI GAATGC 2 cut(s) 725, 1257
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 3 cut(s) 1505, 1532, 1708
PfoI TCCNGGA 1 cut(s) 1273
PleI GAGTC 4 cut(s) 413, 1097, 1549, 1750
PluTI GGCGCC 1 cut(s) 1462
PmaCI CACGTG 1 cut(s) 778
PmlI CACGTG 1 cut(s) 778
PpsI GAGTC 4 cut(s) 413, 1097, 1549, 1750
Ppu21I YACGTR 1 cut(s) 778
PpuMI RGGWCCY 1 cut(s) 613
Psp124BI GAGCTC 1 cut(s) 939
Psp5II RGGWCCY 1 cut(s) 613
Psp6I CCWGG 2 cut(s) 431, 904
PspCI CACGTG 1 cut(s) 778
PspFI CCCAGC 1 cut(s) 361
PspGI CCWGG 2 cut(s) 431, 904
PspN4I GGNNCC 4 cut(s) 245, 614, 1279, 1460
PspPI GGNCC 2 cut(s) 613, 1213
PspPPI RGGWCCY 1 cut(s) 613
PstNI CAGNNNCTG 3 cut(s) 686, 890, 1640
PsuI RGATCY 2 cut(s) 662, 1197
PvuII CAGCTG 2 cut(s) 1190, 1739
RseI CAYNNNNRTG 1 cut(s) 1673
SacI GAGCTC 1 cut(s) 939
SapI GCTCTTC 1 cut(s) 1023
SaqAI TTAA 8 cut(s) 23, 839, 942, 987, 993, 1134, 1464, 1520
Sau3AI GATC 5 cut(s) 662, 1170, 1197, 1351, 1796
Sau96I GGNCC 2 cut(s) 613, 1213
SchI GAGTC 4 cut(s) 414, 1097, 1550, 1751
ScrFI CCNGG 4 cut(s) 433, 566, 906, 1275
SduI GDGCHC 2 cut(s) 939, 1664
SfaNI GCATC 6 cut(s) 68, 171, 643, 836, 1288, 1330
SfoI GGCGCC 1 cut(s) 1460
SinI GGWCC 2 cut(s) 613, 1213
SmiMI CAYNNNNRTG 1 cut(s) 1673
SmlI CTYRAG 2 cut(s) 1463, 1519
SmoI CTYRAG 2 cut(s) 1463, 1519
SseBI AGGCCT 1 cut(s) 1720
SsiI CCGC 8 cut(s) 160, 169, 323, 569, 579, 783, 1110, 1483
SspDI GGCGCC 1 cut(s) 1458
SspMI CTAG 4 cut(s) 585, 1124, 1509, 1804
SstI GAGCTC 1 cut(s) 939
StuI AGGCCT 1 cut(s) 1720
StyD4I CCNGG 4 cut(s) 431, 564, 904, 1273
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 9 cut(s) 181, 302, 1075, 1217, 1398, 1417, 1636, 1703, 1749
TaiI ACGT 3 cut(s) 780, 848, 984
TaqI TCGA 3 cut(s) 69, 204, 732
TauI GCSGC 5 cut(s) 162, 326, 581, 785, 1113
TfiI GAWTC 3 cut(s) 1505, 1532, 1708
Tru1I TTAA 8 cut(s) 23, 839, 942, 987, 993, 1134, 1464, 1520
Tru9I TTAA 8 cut(s) 23, 839, 942, 987, 993, 1134, 1464, 1520
TscAI CASTG 7 cut(s) 112, 325, 415, 483, 891, 924, 1752
TseFI GTSAC 5 cut(s) 225, 406, 794, 1100, 1743
TseI GCWGC 8 cut(s) 77, 80, 425, 428, 460, 463, 581, 1451
Tsp45I GTSAC 5 cut(s) 225, 406, 794, 1100, 1743
TspDTI ATGAA 4 cut(s) 563, 575, 1691, 1753
TspGWI ACGGA 1 cut(s) 1088
TspRI CASTG 7 cut(s) 112, 325, 415, 483, 891, 924, 1752
Vha464I CTTAAG 2 cut(s) 1463, 1519
VpaK11BI GGWCC 2 cut(s) 613, 1213
XapI RAATTY 2 cut(s) 1290, 1679
XbaI TCTAGA 1 cut(s) 1123
XspI CTAG 4 cut(s) 585, 1124, 1509, 1804
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.