pycom14g17190

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006789
Physical Location & Seq
Forward (+)
19237656 .. 19242549
4894 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1965 bp
ATGGAGATCACAGAAGAACAAAGAAGACGAGCCGAAGCGAATCGATTAGCAGCACTAGCGAAACGTAAAGCGCTGATCGAATCCGAATCCTCCAATCTTCAACAACAGCAGCAAAACCCGTGGTCACTCTTCAAGTGCCGAAAGATTTCTCCCGACCTCACCCCAAGCTCCACGCATTGCATCCCCGATCCGAGACCCGAAACTTCCCGGGTCAAACCGGTTCCGGATATCCAATTTCCAGCGAGGTTCCGTGTCAGGCTCGAAATCTGCTCCCCGGACTCCTTCTCAGCCACCCCAGTTGCCGTAAAAGGGTTTGCATTTCCGGGGCAGGAGGAGTGCTTAAGGAGACTAAGTGATTGCTTGGCTAATGTCATGCCATCCCATTACACGCAAAATCATGGTGGTGGAAAGGCCGGTGTTTATAAGCTGAGTGATTACAGTGCCGTCTTGAAATGTCTAAGAAGCAGCAAGAGCATTGACACCGAAGAGATACCTTGGGGCACTCTTAATGTTGTAGAGAGACTTTCACATTCTTTTGTTGCGGGAAAATGGATACCATGCAGGCCAGAACACTTGTCGGACGAAATGGTTGATGAGTTCATTGGGAAGCTACCGAAGAAGCTGTTAGATGCACTTTTGCCTTTCCAACATGAGGGTGTTAGATTTGGTTTGCAGAGAGGTGGACGATGTCTTATTGCAGATGAAATGGGCCTTGGAAAGACACTCCAGGCTATTGCTATTGCAAGCTGTTTCATGCATGAAGGTTCCATACTTGTTGTTTGCCCTGCCATTTTGCGTTACTCTTGGGCAGAAGAACTAGAGCGGTGGCTTCCGTTTTGCTTGCCAGCTGACATCCATCTAGTATTTGGTCATGAAAACAATCCTGCCAATTTAAAGAGATGGCCCAGAGTTGTGGTTATTTCTTACACAATGCTCCACCGGTTGCATAAGAGCATGCTTGAACGAGAATGGGCTCTCTTAGTTGTTGATGAATCCCACCACATACGATGTACCAAGAAATCATCAGAACCTAGAGAGATAAAAGCTGTTCTTGACATTGCAACAAGGGTCAAGCGCATAGTTTTGCTATCTGGAACACCTTCTTTGTCAAGGCCGTATGACATTTTTCATCAGATAGATATGTTATGGCCTGGTTTGCTGGGAAAAGACAAGTATGACTTTGCCAAAACTTACTGTGATGTCAAATATATCCACGGTGTTCAAGGGAAAACTTTTCAGGATTTCTCTAAGGGCACTCGTTTGGAGGAGTTGAACATGTTACTGAAGCAAACGGTTATGATAAGACGCTTGAAGGAACATGTCCTGGTTCAGCTACCACCCAAACGCCGGCAGATTATACAAGTAGTACTGAAGAAATCAGATATAGCTTCAGCAATAGCTGCTGTCAGGGTGGGAAGAAGTGCCACTAAGAATGCATATTCAGAAAATTTGGATGAACCAAATGATAGTGAGGGTTGCTGCAGATCAGGGCAACTTTCCTACCAAGAATTAGGTATTGCAAAGCTAGCTGGGTTTCGGGAATGGCTCTCCTTTCATCCGCTCATTGGAGAATCAGATGGTGTTGCAAACTTGGAATCAGATTCCAGTTCTCACAAAATGTTAATATTTGCGCATCACCATAAAGTACTTGACGGATTACAGGAGTTCATATGCGAGAAAGGGATTACTTTTGTCCGAATTGATGGAAATACACTTGCCGCAGATAGGCAGGAAGCTGTACAATCATTCCAATTATCTACCGAGGTCAAGATTGCAATAATTGGTATTACTGCTGGAGGTGTTGGCCTTGATTTCTCATCAGCAAAGCATGTTGTGTTCTTGGAACTGCCTCAGTCTCCATCGTTGATGCTTCAGGCTGAGGATAGAGCTCACAGGAGAGGGCAAACAAATGCAGTCAACATATACATATTTTGCGGAAAGGTGCTTGTTTCTTTTGTTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

655

Amino Acids

73.54

Weight (kDa)

8.76

Isoelectric Point (pI)

53.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 215 - 460 3.4e-31 SNF2-related domain
ResIII PF04851 225 - 367 9.8e-08 Type III restriction enzyme, res subunit
DEAD PF00270 226 - 371 7.7e-07 DEAD/DEAH box helicase
Helicase_C PF00271 533 - 634 1e-17 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 423
Acc16I TGCGCA 1 cut(s) 1632
AccBSI CCGCTC 2 cut(s) 823, 1561
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 5 cut(s) 542, 823, 1559, 1719, 1935
AclWI GGATC 1 cut(s) 182
AcsI RAATTY 1 cut(s) 1447
AcuI CTGAAG 4 cut(s) 1304, 1374, 1391, 1856
AfaI GTAC 4 cut(s) 1012, 1368, 1647, 1740
AfeI AGCGCT 1 cut(s) 72
AfiI CCNNNNNNNGG 5 cut(s) 309, 652, 1347, 1565, 1725
AflII CTTAAG 1 cut(s) 340
AflIII ACRYGT 2 cut(s) 1275, 1318
AgeI ACCGGT 2 cut(s) 217, 939
AgsI TTSAA 7 cut(s) 101, 133, 451, 962, 1223, 1273, 1312
AjnI CCWGG 3 cut(s) 726, 1150, 1323
AjuI GAANNNNNNNTTGG 2 cut(s) 696, 728
Alw21I GWGCWC 1 cut(s) 1891
Alw26I GTCTC 4 cut(s) 187, 340, 514, 1860
AlwI GGATC 1 cut(s) 182
Ama87I CYCGRG 1 cut(s) 207
Aor13HI TCCGGA 1 cut(s) 223
Aor51HI AGCGCT 1 cut(s) 72
AoxI GGCC 7 cut(s) 411, 563, 709, 902, 1112, 1148, 1804
ApeKI GCWGC 5 cut(s) 50, 109, 465, 1400, 1479
ApoI RAATTY 1 cut(s) 1447
AsiGI ACCGGT 2 cut(s) 217, 939
Asp700I GAANNNNTTC 2 cut(s) 145, 1099
AspLEI GCGC 3 cut(s) 73, 1077, 1633
AspS9I GGNCC 2 cut(s) 709, 903
AsuC2I CCSGG 4 cut(s) 208, 209, 275, 324
AsuHPI GGTGA 2 cut(s) 151, 1628
AsuNHI GCTAGC 1 cut(s) 1525
AvaI CYCGRG 1 cut(s) 207
BaeGI GKGCMC 2 cut(s) 503, 1256
BanII GRGCYC 2 cut(s) 976, 1891
BarI GAAGNNNNNNTAC 2 cut(s) 753, 785
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 1891
BbvCI CCTCAGC 1 cut(s) 1878
BbvI GCAGC 5 cut(s) 62, 121, 477, 1387, 1466
BccI CCATC 6 cut(s) 385, 864, 894, 1571, 1697, 1867
BceAI ACGGC 3 cut(s) 287, 428, 1099
BciT130I CCWGG 3 cut(s) 728, 1152, 1325
BciVI GTATCC 1 cut(s) 546
BcnI CCSGG 4 cut(s) 208, 209, 275, 324
BcoDI GTCTC 4 cut(s) 187, 340, 514, 1860
BfaI CTAG 5 cut(s) 56, 818, 860, 1032, 1526
BfmI CTRYAG 1 cut(s) 1480
BfoI RGCGCY 1 cut(s) 74
BfrI CTTAAG 1 cut(s) 340
BfuI GTATCC 1 cut(s) 546
BisI GCNGC 6 cut(s) 51, 110, 466, 1401, 1480, 1719
BlsI GCNGC 6 cut(s) 52, 111, 467, 1402, 1481, 1720
BmcAI AGTACT 2 cut(s) 1368, 1647
Bme1390I CCNGG 7 cut(s) 208, 209, 275, 324, 728, 1152, 1325
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 2 cut(s) 709, 903
BmiI GGNNCC 3 cut(s) 222, 248, 766
BmrFI CCNGG 7 cut(s) 208, 209, 275, 324, 728, 1152, 1325
BmrI ACTGGG 1 cut(s) 290
BmsI GCATC 4 cut(s) 189, 619, 1642, 1857
BmtI GCTAGC 1 cut(s) 1529
BmuI ACTGGG 1 cut(s) 290
BpiI GAAGAC 1 cut(s) 31
BpmI CTGGAG 2 cut(s) 710, 1815
Bpu10I CCTNAGC 1 cut(s) 1878
BpuMI CCSGG 4 cut(s) 208, 209, 275, 324
Bsa29I ATCGAT 1 cut(s) 43
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 8 cut(s) 119, 207, 273, 323, 494, 712, 1213, 1761
BsaWI WCCGGW 3 cut(s) 217, 223, 939
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 5 cut(s) 309, 652, 1347, 1565, 1725
Bse118I RCCGGY 4 cut(s) 217, 413, 939, 1347
Bse1I ACTGG 2 cut(s) 296, 1605
Bse3DI GCAATG 2 cut(s) 175, 1056
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 3 cut(s) 728, 1152, 1325
BseCI ATCGAT 1 cut(s) 43
BseDI CCNNGG 8 cut(s) 119, 207, 273, 323, 494, 712, 1213, 1761
BseGI GGATG 5 cut(s) 180, 377, 852, 1459, 1555
BseLI CCNNNNNNNGG 5 cut(s) 309, 652, 1347, 1565, 1725
BseMI GCAATG 2 cut(s) 175, 1056
BseMII CTCAG 4 cut(s) 300, 419, 1865, 1869
BseNI ACTGG 2 cut(s) 296, 1605
BseRI GAGGAG 2 cut(s) 347, 1280
BseSI GKGCMC 2 cut(s) 503, 1256
BseXI GCAGC 5 cut(s) 62, 121, 477, 1387, 1466
BseYI CCCAGC 2 cut(s) 1159, 1529
BshFI GGCC 7 cut(s) 413, 565, 711, 904, 1114, 1150, 1806
BshTI ACCGGT 2 cut(s) 217, 939
BshVI ATCGAT 1 cut(s) 43
BsiHKAI GWGCWC 1 cut(s) 1891
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 8 cut(s) 208, 218, 224, 275, 323, 414, 940, 1348
BslI CCNNNNNNNGG 5 cut(s) 309, 652, 1347, 1565, 1725
BsmAI GTCTC 4 cut(s) 187, 340, 514, 1860
BsmI GAATGC 1 cut(s) 1438
BsnI GGCC 7 cut(s) 413, 565, 711, 904, 1114, 1150, 1806
Bso31I GGTCTC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 207
Bsp1286I GDGCHC 4 cut(s) 503, 976, 1256, 1891
Bsp13I TCCGGA 1 cut(s) 223
Bsp1407I TGTACA 1 cut(s) 1738
Bsp143I GATC 4 cut(s) 6, 75, 187, 1484
BspACI CCGC 5 cut(s) 542, 823, 1559, 1719, 1935
BspANI GGCC 7 cut(s) 413, 565, 711, 904, 1114, 1150, 1806
BspCNI CTCAG 4 cut(s) 299, 420, 1864, 1870
BspDI ATCGAT 1 cut(s) 43
BspEI TCCGGA 1 cut(s) 223
BspHI TCATGA 1 cut(s) 871
BspLI GGNNCC 3 cut(s) 222, 248, 766
BspMAI CTGCAG 1 cut(s) 1484
BspOI GCTAGC 1 cut(s) 1529
BspPI GGATC 1 cut(s) 182
BspTI CTTAAG 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 187
BsrBI CCGCTC 2 cut(s) 823, 1561
BsrDI GCAATG 2 cut(s) 175, 1056
BsrFI RCCGGY 4 cut(s) 217, 413, 939, 1347
BsrGI TGTACA 1 cut(s) 1738
BsrI ACTGG 2 cut(s) 296, 1605
BssAI RCCGGY 4 cut(s) 217, 413, 939, 1347
BssECI CCNNGG 8 cut(s) 119, 207, 273, 323, 494, 712, 1213, 1761
BssMI GATC 4 cut(s) 6, 75, 187, 1484
BssT1I CCWWGG 2 cut(s) 494, 712
Bst2UI CCWGG 3 cut(s) 728, 1152, 1325
Bst4CI ACNGT 4 cut(s) 440, 1196, 1217, 1294
Bst6I CTCTTC 2 cut(s) 134, 480
BstAFI CTTAAG 1 cut(s) 340
BstAPI GCANNNNNTGC 1 cut(s) 1400
BstAUI TGTACA 1 cut(s) 1738
BstC8I GCNNGC 7 cut(s) 563, 745, 842, 846, 956, 1349, 1527
BstDEI CTNAG 9 cut(s) 286, 350, 428, 458, 979, 1248, 1428, 1851, 1878
BstDSI CCRYGG 2 cut(s) 119, 1213
BstF5I GGATG 5 cut(s) 180, 377, 852, 1459, 1555
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 3 cut(s) 73, 1077, 1633
BstKTI GATC 4 cut(s) 9, 78, 190, 1487
BstMAI GTCTC 4 cut(s) 187, 340, 514, 1860
BstMBI GATC 4 cut(s) 6, 75, 187, 1484
BstMWI GCNNNNNNNGC 5 cut(s) 56, 471, 1156, 1400, 1526
BstNI CCWGG 3 cut(s) 728, 1152, 1325
BstNSI RCATGY 4 cut(s) 958, 1279, 1322, 1832
BstSCI CCNGG 7 cut(s) 206, 207, 273, 322, 726, 1150, 1323
BstSFI CTRYAG 1 cut(s) 1480
BstSLI GKGCMC 2 cut(s) 503, 1256
BstV1I GCAGC 5 cut(s) 62, 121, 477, 1387, 1466
BstV2I GAAGAC 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 913
Bsu15I ATCGAT 1 cut(s) 43
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 7 cut(s) 413, 565, 711, 904, 1114, 1150, 1806
BsuTUI ATCGAT 1 cut(s) 43
BtgI CCRYGG 2 cut(s) 119, 1213
BtsCI GGATG 5 cut(s) 180, 377, 852, 1459, 1555
BtsIMutI CAGTG 1 cut(s) 445
Cac8I GCNNGC 7 cut(s) 563, 745, 842, 846, 956, 1349, 1527
CciI TCATGA 1 cut(s) 871
CfoI GCGC 3 cut(s) 73, 1077, 1633
Cfr10I RCCGGY 4 cut(s) 217, 413, 939, 1347
Cfr13I GGNCC 2 cut(s) 709, 903
Cfr9I CCCGGG 1 cut(s) 207
ClaI ATCGAT 1 cut(s) 43
CseI GACGC 1 cut(s) 1314
Csp6I GTAC 4 cut(s) 1011, 1367, 1646, 1739
CspAI ACCGGT 2 cut(s) 217, 939
CspCI CAANNNNNGTGG 4 cut(s) 101, 136, 280, 315
CviQI GTAC 4 cut(s) 1011, 1367, 1646, 1739
DdeI CTNAG 9 cut(s) 286, 350, 428, 458, 979, 1248, 1428, 1851, 1878
DpnI GATC 4 cut(s) 8, 77, 189, 1486
DpnII GATC 4 cut(s) 6, 75, 187, 1484
DraI TTTAAA 1 cut(s) 894
Eam1104I CTCTTC 2 cut(s) 134, 480
EarI CTCTTC 2 cut(s) 134, 480
Ecl136II GAGCTC 1 cut(s) 1889
Eco130I CCWWGG 2 cut(s) 494, 712
Eco24I GRGCYC 2 cut(s) 976, 1891
Eco31I GGTCTC 1 cut(s) 187
Eco32I GATATC 1 cut(s) 229
Eco47III AGCGCT 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 1889
Eco57I CTGAAG 4 cut(s) 1304, 1374, 1391, 1856
Eco88I CYCGRG 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 1889
EcoRII CCWGG 3 cut(s) 726, 1150, 1323
EcoRV GATATC 1 cut(s) 229
EcoT14I CCWWGG 2 cut(s) 494, 712
EcoT22I ATGCAT 2 cut(s) 759, 1438
EcoT38I GRGCYC 2 cut(s) 976, 1891
ErhI CCWWGG 2 cut(s) 494, 712
FalI AAGNNNNNCTT 4 cut(s) 1035, 1067, 1163, 1195
FauI CCCGC 1 cut(s) 535
FauNDI CATATG 1 cut(s) 1670
Fnu4HI GCNGC 6 cut(s) 51, 110, 466, 1401, 1480, 1719
FokI GGATG 5 cut(s) 167, 364, 839, 1466, 1542
FriOI GRGCYC 2 cut(s) 976, 1891
Fsp4HI GCNGC 6 cut(s) 51, 110, 466, 1401, 1480, 1719
FspBI CTAG 5 cut(s) 56, 818, 860, 1032, 1526
FspI TGCGCA 1 cut(s) 1632
GlaI GCGC 3 cut(s) 72, 1076, 1632
GluI GCNGC 6 cut(s) 51, 110, 466, 1401, 1480, 1719
GsaI CCCAGC 2 cut(s) 1163, 1533
GsuI CTGGAG 2 cut(s) 710, 1815
HaeII RGCGCY 1 cut(s) 74
HaeIII GGCC 7 cut(s) 413, 565, 711, 904, 1114, 1150, 1806
HapII CCGG 8 cut(s) 208, 218, 224, 275, 323, 414, 940, 1348
HgaI GACGC 1 cut(s) 1314
HhaI GCGC 3 cut(s) 73, 1077, 1633
Hin6I GCGC 3 cut(s) 71, 1075, 1631
HinP1I GCGC 3 cut(s) 71, 1075, 1631
HincII GTYRAC 1 cut(s) 1918
HindII GTYRAC 1 cut(s) 1918
HinfI GANTC 8 cut(s) 40, 80, 86, 278, 992, 1571, 1595, 1601
HpaII CCGG 8 cut(s) 208, 218, 224, 275, 323, 414, 940, 1348
HphI GGTGA 2 cut(s) 151, 1628
Hpy166II GTNNAC 2 cut(s) 683, 1918
Hpy188III TCNNGA 9 cut(s) 152, 224, 448, 872, 1052, 1092, 1238, 1538, 1768
Hpy8I GTNNAC 2 cut(s) 683, 1918
HpyAV CCTTC 4 cut(s) 292, 755, 1110, 1306
HpyCH4III ACNGT 4 cut(s) 440, 1196, 1217, 1294
HpyCH4IV ACGT 1 cut(s) 64
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 471, 1156, 1400, 1526
HpyF3I CTNAG 9 cut(s) 286, 350, 428, 458, 979, 1248, 1428, 1851, 1878
HpySE526I ACGT 1 cut(s) 64
HspAI GCGC 3 cut(s) 71, 1075, 1631
Kpn2I TCCGGA 1 cut(s) 223
KroI GCCGGC 1 cut(s) 1347
KroNI GCCGGC 1 cut(s) 1349
Kzo9I GATC 4 cut(s) 6, 75, 187, 1484
LmnI GCTCC 3 cut(s) 173, 275, 939
Lsp1109I GCAGC 5 cut(s) 62, 121, 477, 1387, 1466
LweI GCATC 4 cut(s) 189, 619, 1642, 1857
MaeI CTAG 5 cut(s) 56, 818, 860, 1032, 1526
MaeII ACGT 1 cut(s) 64
MaeIII GTNAC 3 cut(s) 123, 797, 1278
MalI GATC 4 cut(s) 8, 77, 189, 1486
MbiI CCGCTC 2 cut(s) 823, 1561
MboI GATC 4 cut(s) 6, 75, 187, 1484
MboII GAAGA 9 cut(s) 26, 36, 89, 121, 497, 628, 824, 1384, 1428
MhlI GDGCHC 4 cut(s) 503, 976, 1256, 1891
MluCI AATT 7 cut(s) 233, 889, 1447, 1508, 1698, 1751, 1779
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 2 cut(s) 558, 670
Mph1103I ATGCAT 2 cut(s) 759, 1438
MroI TCCGGA 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 1347
MroXI GAANNNNTTC 2 cut(s) 145, 1099
MseI TTAA 4 cut(s) 341, 507, 893, 1622
MslI CAYNNNNRTG 2 cut(s) 402, 654
MspA1I CMGCKG 1 cut(s) 848
MspCI CTTAAG 1 cut(s) 340
MspI CCGG 8 cut(s) 208, 218, 224, 275, 323, 414, 940, 1348
MspR9I CCNGG 7 cut(s) 208, 209, 275, 324, 728, 1152, 1325
Mva1269I GAATGC 1 cut(s) 1438
MvaI CCWGG 3 cut(s) 728, 1152, 1325
MwoI GCNNNNNNNGC 5 cut(s) 56, 471, 1156, 1400, 1526
NaeI GCCGGC 1 cut(s) 1349
NciI CCSGG 4 cut(s) 208, 209, 275, 324
NdeI CATATG 1 cut(s) 1670
NdeII GATC 4 cut(s) 6, 75, 187, 1484
NgoMIV GCCGGC 1 cut(s) 1347
NheI GCTAGC 1 cut(s) 1525
NlaIV GGNNCC 3 cut(s) 222, 248, 766
NmuCI GTSAC 1 cut(s) 123
NsbI TGCGCA 1 cut(s) 1632
NsiI ATGCAT 2 cut(s) 759, 1438
NspI RCATGY 4 cut(s) 958, 1279, 1322, 1832
PaeI GCATGC 1 cut(s) 958
PagI TCATGA 1 cut(s) 871
PciI ACATGT 2 cut(s) 1275, 1318
PctI GAATGC 1 cut(s) 1438
PdiI GCCGGC 1 cut(s) 1349
PdmI GAANNNNTTC 2 cut(s) 145, 1099
PfeI GAWTC 7 cut(s) 40, 80, 86, 992, 1571, 1595, 1601
PflFI GACNNNGTC 1 cut(s) 687
PinAI ACCGGT 2 cut(s) 217, 939
PkrI GCNGC 6 cut(s) 52, 111, 467, 1402, 1481, 1720
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PscI ACATGT 2 cut(s) 1275, 1318
PsiI TTATAA 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 1891
Psp6I CCWGG 3 cut(s) 726, 1150, 1323
PspFI CCCAGC 2 cut(s) 1159, 1529
PspGI CCWGG 3 cut(s) 726, 1150, 1323
PspN4I GGNNCC 3 cut(s) 222, 248, 766
PspPI GGNCC 2 cut(s) 709, 903
PstI CTGCAG 1 cut(s) 1484
PsyI GACNNNGTC 1 cut(s) 687
PvuII CAGCTG 1 cut(s) 848
RsaI GTAC 4 cut(s) 1012, 1368, 1647, 1740
RsaNI GTAC 4 cut(s) 1011, 1367, 1646, 1739
RseI CAYNNNNRTG 2 cut(s) 402, 654
SacI GAGCTC 1 cut(s) 1891
SaqAI TTAA 4 cut(s) 341, 507, 893, 1622
SatI GCNGC 6 cut(s) 51, 110, 466, 1401, 1480, 1719
Sau3AI GATC 4 cut(s) 6, 75, 187, 1484
Sau96I GGNCC 2 cut(s) 709, 903
ScaI AGTACT 2 cut(s) 1368, 1647
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 7 cut(s) 208, 209, 275, 324, 728, 1152, 1325
SduI GDGCHC 4 cut(s) 503, 976, 1256, 1891
SfaNI GCATC 4 cut(s) 189, 619, 1642, 1857
SfcI CTRYAG 1 cut(s) 1480
SmaI CCCGGG 1 cut(s) 209
SmiMI CAYNNNNRTG 2 cut(s) 402, 654
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
SphI GCATGC 1 cut(s) 958
Sse9I AATT 7 cut(s) 233, 889, 1447, 1508, 1698, 1751, 1779
SsiI CCGC 5 cut(s) 542, 823, 1559, 1719, 1935
SspI AATATT 1 cut(s) 1626
SspMI CTAG 5 cut(s) 56, 818, 860, 1032, 1526
SstI GAGCTC 1 cut(s) 1891
StyD4I CCNGG 7 cut(s) 206, 207, 273, 322, 726, 1150, 1323
StyI CCWWGG 2 cut(s) 494, 712
TaaI ACNGT 4 cut(s) 440, 1196, 1217, 1294
TaiI ACGT 1 cut(s) 67
TaqI TCGA 3 cut(s) 43, 78, 261
TasI AATT 7 cut(s) 233, 889, 1447, 1508, 1698, 1751, 1779
TatI WGTACW 3 cut(s) 1366, 1645, 1738
TauI GCSGC 1 cut(s) 1721
TfiI GAWTC 7 cut(s) 40, 80, 86, 992, 1571, 1595, 1601
Tru1I TTAA 4 cut(s) 341, 507, 893, 1622
Tru9I TTAA 4 cut(s) 341, 507, 893, 1622
TscAI CASTG 1 cut(s) 445
TseFI GTSAC 1 cut(s) 123
TseI GCWGC 5 cut(s) 50, 109, 465, 1400, 1479
Tsp45I GTSAC 1 cut(s) 123
TspGWI ACGGA 3 cut(s) 239, 822, 1668
TspMI CCCGGG 1 cut(s) 207
TspRI CASTG 1 cut(s) 445
Tth111I GACNNNGTC 1 cut(s) 687
Vha464I CTTAAG 1 cut(s) 340
XapI RAATTY 1 cut(s) 1447
XceI RCATGY 4 cut(s) 958, 1279, 1322, 1832
XcmI CCANNNNNNNNNTGG 1 cut(s) 863
XmaI CCCGGG 1 cut(s) 207
XmnI GAANNNNTTC 2 cut(s) 145, 1099
XspI CTAG 5 cut(s) 56, 818, 860, 1032, 1526
ZrmI AGTACT 2 cut(s) 1368, 1647
Zsp2I ATGCAT 2 cut(s) 759, 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.