pycom14g17200

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006789
Physical Location & Seq
Forward (+)
19243058 .. 19246085
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1491 bp
ATGATACCGGCGTATGATAAATTAGTTGCAAACATTCTACATGGATCTGCACAGCACAATGATAGTGATGGAATTTCATCCCAAACGCACAGTAGTACCAAGGCTCAAGTGGTTTCTGATTGCAATGGGGACAAGGGATGTTCAGAAGAGAACTTGGAAGGAGATGTTTCTACGAGCAGTGAAGACAAAGATGGACTTGAAAAGGAACACCAAAACAACTGTCAAGCAACCAAATCAAAGGACGCCGAACCAGTTCAGCCAACGGAAGCTAAAGAAAGTAGTACCAACCAAGTAGATGCTTTGCGATTTGAGGTGAGCCAATACACTGGTAGAATCCACTTGTATTCTTGCATCTCGGGAGAGGATTCAAGACCAAGGCCACTTTTTGAGAATTTTAGACCAGAAGAAATCGAGGCATTGAATTCTACTGCTGCTGACAATATTAAAGGAACTTCTTTCAAATTTTTGAAGGAGAATCCAGGTTATTTGCATGCTTTTCTTGCATTCATGGAAGAATGGAAGAAACTGAGGCCCATCGAACAAAAAAAATTACTTGGAAAGCCTTTGCAGCTTCCTTTGACTATTGAGTTGTGCTACCTGTGCGAAGGCATTAACCACGACATCAGGGGACTACTGAAAGGTGGAAGCAAGCGCCGCTCTACCCCTTTTGTTGAGATCAGCAAACCTTTACCATCAAACGCTGAGTGGAAAAAAGTCCGCCTACGTAGTGGCTATGGAAAAAAGGAGAAGGAATACACTCAGGGTTTTACTCTGACTGATGATCCTCTGTGCAAACTCTGTCAATCACCATGCACGGGCCATAATGCCAAGACACCTGAATATTTTGAAGATCTGTTCTGCCAGCTTAGCTGCTACGAAGAATACCGCATCCGAACAAGTAATAGATCTCTTCGTCATGAGCTTTTTCAACTGGAACGTGGTGTCTGCACAATTTGTCAATTAGATTGTCATAAACTTGTTGAGCACCTAAGACCGTTATCATTGGCAATGAGGAGGCAGTATATTGAAAAATTTGCGCCACGAGTGGCAATTCTGAAGAAACCGCTTGATAGGCTTGTTAAGGATCCGAAAGAGGGCAATGCATGGCATGCAGACCACTTTGTTCCTGTCTATCGAGGTGGAGGTGAATGCAGGCTCGAGAACATGAGAACACTTTGTGTGGCCTGTCATCGCGACGTTACTAAAGCACAATGTGCTGAACGGCTGTCAACAAGGATCCGGGCTAAGAAAAAACTGAAAGCAATCATGAACGACATTAAAAAGAAAGCTACGGAGACTAATCTAGCGGGCCCGGAACATATAGAGATTATGCAAGAGGATGCAATTGAGGATGAGCTTTTCGTCAAGGTTCCCGGGAGCGCCTACTCATTGGAAAATCGCTTGGATGCTACAACTACAGGCGAAGACTTGGACAAGCCCTCCAGTAGCAACAAGGTTCCAACGGTAGATAGCCATCTGTTAGGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

497

Amino Acids

55.96

Weight (kDa)

6.96

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HNH PF01844 365 - 401 3.1e-06 HNH endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 657
AccII CGCG 1 cut(s) 1194
AciI CCGC 5 cut(s) 655, 718, 886, 1064, 1307
AclWI GGATC 6 cut(s) 52, 776, 1079, 1092, 1231, 1244
AcsI RAATTY 5 cut(s) 72, 391, 421, 461, 1031
AcuI CTGAAG 1 cut(s) 1076
AcyI GRCGYC 1 cut(s) 243
AdeI CACNNNGTG 2 cut(s) 1178, 1214
AfaI GTAC 2 cut(s) 97, 283
AfiI CCNNNNNNNGG 2 cut(s) 815, 1094
AgsI TTSAA 8 cut(s) 200, 369, 421, 460, 469, 848, 929, 1028
AjnI CCWGG 1 cut(s) 478
AluBI AGCT 7 cut(s) 269, 571, 865, 870, 922, 1289, 1357
AluI AGCT 7 cut(s) 269, 571, 865, 870, 922, 1289, 1357
Alw21I GWGCWC 1 cut(s) 987
Alw26I GTCTC 1 cut(s) 1289
AlwI GGATC 6 cut(s) 52, 776, 1079, 1092, 1231, 1244
Ama87I CYCGRG 3 cut(s) 355, 1157, 1373
AoxI GGCC 5 cut(s) 377, 530, 817, 1182, 1309
ApaI GGGCCC 1 cut(s) 1313
ApeKI GCWGC 3 cut(s) 431, 568, 870
ApoI RAATTY 5 cut(s) 72, 391, 421, 461, 1031
ArsI GACNNNNNNTTYG 2 cut(s) 205, 237
Asp700I GAANNNNTTC 1 cut(s) 252
AspLEI GCGC 3 cut(s) 654, 1039, 1382
AspS9I GGNCC 4 cut(s) 531, 817, 1309, 1310
AsuC2I CCSGG 4 cut(s) 1241, 1313, 1374, 1375
AsuHPI GGTGA 3 cut(s) 325, 798, 1157
AvaI CYCGRG 3 cut(s) 355, 1157, 1373
BaeGI GKGCMC 1 cut(s) 1313
BaeI ACNNNNGTAYC 3 cut(s) 29, 79, 112
BamHI GGATCC 2 cut(s) 1084, 1236
BanII GRGCYC 1 cut(s) 1313
BauI CACGAG 1 cut(s) 1041
BbsI GAAGAC 2 cut(s) 189, 1431
Bbv12I GWGCWC 1 cut(s) 987
BbvI GCAGC 3 cut(s) 418, 580, 857
BccI CCATC 5 cut(s) 62, 185, 542, 700, 1482
BceAI ACGGC 1 cut(s) 1238
BciT130I CCWGG 1 cut(s) 480
BcnI CCSGG 4 cut(s) 1241, 1313, 1374, 1375
BcoDI GTCTC 1 cut(s) 1289
BfaI CTAG 1 cut(s) 1304
BfmI CTRYAG 1 cut(s) 1416
BfoI RGCGCY 2 cut(s) 655, 1383
BglII AGATCT 2 cut(s) 850, 905
BisI GCNGC 4 cut(s) 432, 569, 655, 871
BlpI GCTNAGC 1 cut(s) 866
BlsI GCNGC 4 cut(s) 433, 570, 656, 872
Bme1390I CCNGG 5 cut(s) 480, 1241, 1313, 1374, 1375
BmeT110I CYCGRG 3 cut(s) 355, 1157, 1373
BmgT120I GGNCC 4 cut(s) 531, 817, 1309, 1310
BmiI GGNNCC 5 cut(s) 1086, 1238, 1311, 1371, 1458
BmrFI CCNGG 5 cut(s) 480, 1241, 1313, 1374, 1375
BmsI GCATC 5 cut(s) 286, 360, 897, 1330, 1396
BpiI GAAGAC 2 cut(s) 189, 1431
BpmI CTGGAG 1 cut(s) 1426
Bpu1102I GCTNAGC 1 cut(s) 866
BpuEI CTTGAG 1 cut(s) 90
BpuMI CCSGG 4 cut(s) 1241, 1313, 1374, 1375
BsaAI YACGTR 1 cut(s) 725
BsaBI GATNNNNATC 1 cut(s) 1473
BsaHI GRCGYC 1 cut(s) 243
BsaJI CCNNGG 3 cut(s) 99, 374, 1373
BsaXI ACNNNNNCTCC 2 cut(s) 1424, 1454
Bsc4I CCNNNNNNNGG 2 cut(s) 815, 1094
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 4 cut(s) 251, 331, 936, 1443
Bse3DI GCAATG 3 cut(s) 130, 1014, 1105
Bse8I GATNNNNATC 1 cut(s) 1473
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 3 cut(s) 99, 374, 1373
BseGI GGATG 6 cut(s) 77, 143, 888, 1345, 1357, 1411
BseJI GATNNNNATC 1 cut(s) 1473
BseLI CCNNNNNNNGG 2 cut(s) 815, 1094
BseMI GCAATG 3 cut(s) 130, 1014, 1105
BseMII CTCAG 3 cut(s) 518, 693, 773
BseNI ACTGG 4 cut(s) 251, 331, 936, 1443
BseRI GAGGAG 1 cut(s) 1027
BseSI GKGCMC 1 cut(s) 1313
BseXI GCAGC 3 cut(s) 418, 580, 857
BsgI GTGCAG 2 cut(s) 33, 931
Bsh1236I CGCG 1 cut(s) 1194
BshFI GGCC 5 cut(s) 379, 532, 819, 1184, 1311
BsiHKAI GWGCWC 1 cut(s) 987
BsiHKCI CYCGRG 3 cut(s) 355, 1157, 1373
BsiSI CCGG 4 cut(s) 8, 1240, 1313, 1374
BslFI GGGAC 2 cut(s) 143, 642
BslI CCNNNNNNNGG 2 cut(s) 815, 1094
BsmAI GTCTC 1 cut(s) 1289
BsmFI GGGAC 2 cut(s) 143, 642
BsmI GAATGC 2 cut(s) 503, 1154
BsnI GGCC 5 cut(s) 379, 532, 819, 1184, 1311
BsoBI CYCGRG 3 cut(s) 355, 1157, 1373
Bsp120I GGGCCC 1 cut(s) 1309
Bsp1286I GDGCHC 2 cut(s) 987, 1313
Bsp143I GATC 7 cut(s) 44, 675, 781, 850, 905, 1084, 1236
Bsp1720I GCTNAGC 1 cut(s) 866
Bsp68I TCGCGA 1 cut(s) 1194
BspACI CCGC 5 cut(s) 655, 718, 886, 1064, 1307
BspANI GGCC 5 cut(s) 379, 532, 819, 1184, 1311
BspCNI CTCAG 3 cut(s) 519, 694, 772
BspFNI CGCG 1 cut(s) 1194
BspHI TCATGA 2 cut(s) 916, 1266
BspLI GGNNCC 5 cut(s) 1086, 1238, 1311, 1371, 1458
BspPI GGATC 6 cut(s) 52, 776, 1079, 1092, 1231, 1244
BsrBI CCGCTC 1 cut(s) 657
BsrDI GCAATG 3 cut(s) 130, 1014, 1105
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 4 cut(s) 251, 331, 936, 1443
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 3 cut(s) 99, 374, 1373
BssMI GATC 7 cut(s) 44, 675, 781, 850, 905, 1084, 1236
BssNI GRCGYC 1 cut(s) 243
BssSI CACGAG 1 cut(s) 1041
BssT1I CCWWGG 2 cut(s) 99, 374
Bst2BI CACGAG 1 cut(s) 1041
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 4 cut(s) 92, 221, 996, 1465
Bst6I CTCTTC 2 cut(s) 141, 915
BstACI GRCGYC 1 cut(s) 243
BstAPI GCANNNNNTGC 2 cut(s) 1109, 1214
BstBAI YACGTR 1 cut(s) 725
BstC8I GCNNGC 6 cut(s) 492, 650, 863, 1110, 1154, 1309
BstDEI CTNAG 6 cut(s) 527, 702, 759, 866, 989, 1245
BstF5I GGATG 6 cut(s) 77, 143, 888, 1345, 1357, 1411
BstFNI CGCG 1 cut(s) 1194
BstH2I RGCGCY 2 cut(s) 655, 1383
BstHHI GCGC 3 cut(s) 654, 1039, 1382
BstKTI GATC 7 cut(s) 47, 678, 784, 853, 908, 1087, 1239
BstMAI GTCTC 1 cut(s) 1289
BstMBI GATC 7 cut(s) 44, 675, 781, 850, 905, 1084, 1236
BstMWI GCNNNNNNNGC 8 cut(s) 500, 568, 600, 654, 867, 1072, 1109, 1214
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 2 cut(s) 494, 1112
BstSCI CCNGG 5 cut(s) 478, 1239, 1311, 1372, 1373
BstSFI CTRYAG 1 cut(s) 1416
BstSLI GKGCMC 1 cut(s) 1313
BstSNI TACGTA 1 cut(s) 725
BstUI CGCG 1 cut(s) 1194
BstV1I GCAGC 3 cut(s) 418, 580, 857
BstV2I GAAGAC 2 cut(s) 189, 1431
BstX2I RGATCY 5 cut(s) 44, 850, 905, 1084, 1236
BstXI CCANNNNNNTGG 1 cut(s) 326
BstYI RGATCY 5 cut(s) 44, 850, 905, 1084, 1236
BsuRI GGCC 5 cut(s) 379, 532, 819, 1184, 1311
BtgZI GCGATG 1 cut(s) 1175
BtsCI GGATG 6 cut(s) 77, 143, 888, 1345, 1357, 1411
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 2 cut(s) 184, 324
BtuMI TCGCGA 1 cut(s) 1194
Cac8I GCNNGC 6 cut(s) 492, 650, 863, 1110, 1154, 1309
CciI TCATGA 2 cut(s) 916, 1266
CfoI GCGC 3 cut(s) 654, 1039, 1382
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 4 cut(s) 531, 817, 1309, 1310
Cfr9I CCCGGG 1 cut(s) 1373
CseI GACGC 1 cut(s) 251
Csp6I GTAC 2 cut(s) 96, 282
CviAII CATG 9 cut(s) 41, 491, 508, 810, 917, 1104, 1109, 1165, 1267
CviQI GTAC 2 cut(s) 96, 282
DdeI CTNAG 6 cut(s) 527, 702, 759, 866, 989, 1245
DpnI GATC 7 cut(s) 46, 677, 783, 852, 907, 1086, 1238
DpnII GATC 7 cut(s) 44, 675, 781, 850, 905, 1084, 1236
DraIII CACNNNGTG 2 cut(s) 1178, 1214
Eam1104I CTCTTC 2 cut(s) 141, 915
EarI CTCTTC 2 cut(s) 141, 915
EciI GGCGGA 1 cut(s) 707
Eco105I TACGTA 1 cut(s) 725
Eco130I CCWWGG 2 cut(s) 99, 374
Eco24I GRGCYC 1 cut(s) 1313
Eco57I CTGAAG 1 cut(s) 1076
Eco88I CYCGRG 3 cut(s) 355, 1157, 1373
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 2 cut(s) 99, 374
EcoT22I ATGCAT 1 cut(s) 1105
EcoT38I GRGCYC 1 cut(s) 1313
ErhI CCWWGG 2 cut(s) 99, 374
FaeI CATG 9 cut(s) 44, 494, 511, 813, 920, 1107, 1112, 1168, 1270
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 2 cut(s) 143, 642
FatI CATG 9 cut(s) 40, 490, 507, 809, 916, 1103, 1108, 1164, 1266
FauI CCCGC 1 cut(s) 1300
Fnu4HI GCNGC 4 cut(s) 432, 569, 655, 871
FokI GGATG 6 cut(s) 64, 150, 875, 1352, 1364, 1418
FriOI GRGCYC 1 cut(s) 1313
Fsp4HI GCNGC 4 cut(s) 432, 569, 655, 871
FspBI CTAG 1 cut(s) 1304
GlaI GCGC 3 cut(s) 653, 1038, 1381
GluI GCNGC 4 cut(s) 432, 569, 655, 871
GsuI CTGGAG 1 cut(s) 1426
HaeII RGCGCY 2 cut(s) 655, 1383
HaeIII GGCC 5 cut(s) 379, 532, 819, 1184, 1311
HapII CCGG 4 cut(s) 8, 1240, 1313, 1374
HgaI GACGC 1 cut(s) 251
HhaI GCGC 3 cut(s) 654, 1039, 1382
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 9 cut(s) 44, 494, 511, 813, 920, 1107, 1112, 1168, 1270
Hin6I GCGC 3 cut(s) 652, 1037, 1380
HinP1I GCGC 3 cut(s) 652, 1037, 1380
HincII GTYRAC 1 cut(s) 1230
HindII GTYRAC 1 cut(s) 1230
HinfI GANTC 3 cut(s) 333, 365, 475
HpaII CCGG 4 cut(s) 8, 1240, 1313, 1374
HphI GGTGA 3 cut(s) 325, 798, 1157
Hpy166II GTNNAC 1 cut(s) 1230
Hpy188I TCNGA 6 cut(s) 118, 145, 774, 893, 1056, 1089
Hpy188III TCNNGA 6 cut(s) 357, 369, 917, 1159, 1193, 1267
Hpy8I GTNNAC 1 cut(s) 1230
Hpy99I CGWCG 1 cut(s) 1199
HpyAV CCTTC 4 cut(s) 152, 463, 599, 742
HpyCH4III ACNGT 4 cut(s) 92, 221, 996, 1465
HpyCH4IV ACGT 3 cut(s) 724, 937, 1197
HpyF10VI GCNNNNNNNGC 8 cut(s) 500, 568, 600, 654, 867, 1072, 1109, 1214
HpyF3I CTNAG 6 cut(s) 527, 702, 759, 866, 989, 1245
HpySE526I ACGT 3 cut(s) 724, 937, 1197
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 9 cut(s) 44, 494, 511, 813, 920, 1107, 1112, 1168, 1270
HspAI GCGC 3 cut(s) 652, 1037, 1380
Kzo9I GATC 7 cut(s) 44, 675, 781, 850, 905, 1084, 1236
LmnI GCTCC 1 cut(s) 1377
Lsp1109I GCAGC 3 cut(s) 418, 580, 857
LweI GCATC 5 cut(s) 286, 360, 897, 1330, 1396
MaeI CTAG 1 cut(s) 1304
MaeII ACGT 3 cut(s) 724, 937, 1197
MaeIII GTNAC 1 cut(s) 1198
MalI GATC 7 cut(s) 46, 677, 783, 852, 907, 1086, 1238
MbiI CCGCTC 1 cut(s) 657
MboI GATC 7 cut(s) 44, 675, 781, 850, 905, 1084, 1236
MfeI CAATTG 1 cut(s) 1344
MflI RGATCY 5 cut(s) 44, 850, 905, 1084, 1236
MhlI GDGCHC 2 cut(s) 987, 1313
MmeI TCCRAC 1 cut(s) 1484
Mph1103I ATGCAT 1 cut(s) 1105
MroXI GAANNNNTTC 1 cut(s) 252
MseI TTAA 4 cut(s) 444, 612, 1080, 1278
MspI CCGG 4 cut(s) 8, 1240, 1313, 1374
MspR9I CCNGG 5 cut(s) 480, 1241, 1313, 1374, 1375
MunI CAATTG 1 cut(s) 1344
Mva1269I GAATGC 2 cut(s) 503, 1154
MvaI CCWGG 1 cut(s) 480
MvnI CGCG 1 cut(s) 1194
MwoI GCNNNNNNNGC 8 cut(s) 500, 568, 600, 654, 867, 1072, 1109, 1214
NciI CCSGG 4 cut(s) 1241, 1313, 1374, 1375
NdeII GATC 7 cut(s) 44, 675, 781, 850, 905, 1084, 1236
NlaIII CATG 9 cut(s) 44, 494, 511, 813, 920, 1107, 1112, 1168, 1270
NlaIV GGNNCC 5 cut(s) 1086, 1238, 1311, 1371, 1458
NruI TCGCGA 1 cut(s) 1194
NsiI ATGCAT 1 cut(s) 1105
NspI RCATGY 2 cut(s) 494, 1112
PaeI GCATGC 2 cut(s) 494, 1112
PaeR7I CTCGAG 1 cut(s) 1157
PagI TCATGA 2 cut(s) 916, 1266
PctI GAATGC 2 cut(s) 503, 1154
PdmI GAANNNNTTC 1 cut(s) 252
PfeI GAWTC 3 cut(s) 333, 365, 475
PkrI GCNGC 4 cut(s) 433, 570, 656, 872
Ppu21I YACGTR 1 cut(s) 725
Psp6I CCWGG 1 cut(s) 478
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 5 cut(s) 1086, 1238, 1311, 1371, 1458
PspOMI GGGCCC 1 cut(s) 1309
PspPI GGNCC 4 cut(s) 531, 817, 1309, 1310
PsuI RGATCY 5 cut(s) 44, 850, 905, 1084, 1236
RruI TCGCGA 1 cut(s) 1194
RsaI GTAC 2 cut(s) 97, 283
RsaNI GTAC 2 cut(s) 96, 282
SaqAI TTAA 4 cut(s) 444, 612, 1080, 1278
SatI GCNGC 4 cut(s) 432, 569, 655, 871
Sau3AI GATC 7 cut(s) 44, 675, 781, 850, 905, 1084, 1236
Sau96I GGNCC 4 cut(s) 531, 817, 1309, 1310
ScrFI CCNGG 5 cut(s) 480, 1241, 1313, 1374, 1375
SduI GDGCHC 2 cut(s) 987, 1313
SfaNI GCATC 5 cut(s) 286, 360, 897, 1330, 1396
SfcI CTRYAG 1 cut(s) 1416
Sfr274I CTCGAG 1 cut(s) 1157
SlaI CTCGAG 1 cut(s) 1157
SmaI CCCGGG 1 cut(s) 1375
SmlI CTYRAG 2 cut(s) 105, 1157
SmoI CTYRAG 2 cut(s) 105, 1157
SnaBI TACGTA 1 cut(s) 725
SphI GCATGC 2 cut(s) 494, 1112
SsiI CCGC 5 cut(s) 655, 718, 886, 1064, 1307
SspI AATATT 2 cut(s) 442, 842
SspMI CTAG 1 cut(s) 1304
StyD4I CCNGG 5 cut(s) 478, 1239, 1311, 1372, 1373
StyI CCWWGG 2 cut(s) 99, 374
TaaI ACNGT 4 cut(s) 92, 221, 996, 1465
TaiI ACGT 3 cut(s) 727, 940, 1200
TaqI TCGA 4 cut(s) 411, 537, 1135, 1158
TauI GCSGC 1 cut(s) 657
TfiI GAWTC 3 cut(s) 333, 365, 475
Tru1I TTAA 4 cut(s) 444, 612, 1080, 1278
Tru9I TTAA 4 cut(s) 444, 612, 1080, 1278
TscAI CASTG 2 cut(s) 184, 331
TseI GCWGC 3 cut(s) 431, 568, 870
TspDTI ATGAA 3 cut(s) 66, 496, 1283
TspGWI ACGGA 2 cut(s) 278, 1307
TspMI CCCGGG 1 cut(s) 1373
TspRI CASTG 2 cut(s) 184, 331
XapI RAATTY 5 cut(s) 72, 391, 421, 461, 1031
XceI RCATGY 2 cut(s) 494, 1112
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XhoI CTCGAG 1 cut(s) 1157
XmaI CCCGGG 1 cut(s) 1373
XmnI GAANNNNTTC 1 cut(s) 252
XspI CTAG 1 cut(s) 1304
Zsp2I ATGCAT 1 cut(s) 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.