pycom14g19440

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007511
Physical Location & Seq
Reverse (-)
20741550 .. 20750549
9000 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGGATCAAGAATCGTTTCCTATGACAGGAGGAGACGGCCAATACAGCTACGGGAAAAATTCCGGTTCACAGCGGAAGGCAGCTGATACGGCCGAGGGAATGCTTTTTGAGGCCATACTTGAAAACCTACAAATCGAAAATCATCTGTCTCAAGGAAATGCTGTTTCAAACTCGTCATTTTCGATTGCTGATCTAGGTTGTTCTGTTGGTCCCAACACATTCATCGCAGTCAAAAACATCATGGACACCGTGACACAAAAATTCCAGATCGAAGGCCAATCTTCTTCGCTCCCCGAATTCCATGTCTACTTCAACGATCATGTTTCGAACGATTTCAACACTCTTTTCGCCAATCTTTCCCCAGATAGGCCAGGCGATACTATGCTGCGGGTGTGCCTGGTTCTTTCTACGGTCGGTTATTTCCTAATGCTTCTATTCATTTTTTTTATTCAACTTTTGCCCTTCAGTGGCTTTCTAGAGTACCAAAAGAGGTGGCGGACAAAAACAGTCCAGCATGGAACAAAGGACGAATCCATTACTCAAACTCCACTGATGAAGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

188

Amino Acids

20.88

Weight (kDa)

5.1

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 55 - 124 1.3e-24 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 306
AciI CCGC 3 cut(s) 73, 388, 496
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 37, 90
AcsI RAATTY 3 cut(s) 58, 260, 296
AcuI CTGAAG 1 cut(s) 448
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 3 cut(s) 26, 366, 467
AgsI TTSAA 5 cut(s) 122, 168, 313, 337, 452
AjnI CCWGG 2 cut(s) 370, 396
AluBI AGCT 2 cut(s) 48, 83
AluI AGCT 2 cut(s) 48, 83
Alw26I GTCTC 2 cut(s) 27, 153
AlwI GGATC 1 cut(s) 12
AoxI GGCC 5 cut(s) 37, 90, 111, 274, 368
ApeKI GCWGC 2 cut(s) 80, 385
ApoI RAATTY 3 cut(s) 58, 260, 296
ArsI GACNNNNNNTTYG 2 cut(s) 288, 320
Asp700I GAANNNNTTC 2 cut(s) 15, 332
AspS9I GGNCC 1 cut(s) 209
AsuII TTCGAA 1 cut(s) 326
AvaII GGWCC 1 cut(s) 209
BbvI GCAGC 2 cut(s) 92, 372
BceAI ACGGC 2 cut(s) 52, 105
BciT130I CCWGG 2 cut(s) 372, 398
BcoDI GTCTC 2 cut(s) 27, 153
BfaI CTAG 2 cut(s) 194, 476
BisI GCNGC 2 cut(s) 81, 386
BlsI GCNGC 2 cut(s) 82, 387
Bme1390I CCNGG 2 cut(s) 372, 398
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 2 cut(s) 372, 398
Bpu14I TTCGAA 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 62
BsaXI ACNNNNNCTCC 2 cut(s) 529, 559
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 366, 467
BseBI CCWGG 2 cut(s) 372, 398
BseDI CCNNGG 1 cut(s) 93
BseLI CCNNNNNNNGG 3 cut(s) 26, 366, 467
BseRI GAGGAG 1 cut(s) 45
BseX3I CGGCCG 1 cut(s) 90
BseXI GCAGC 2 cut(s) 92, 372
Bsh1285I CGRYCG 2 cut(s) 93, 414
BshFI GGCC 5 cut(s) 39, 92, 113, 276, 370
BsiEI CGRYCG 2 cut(s) 93, 414
BsiSI CCGG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 3 cut(s) 26, 366, 467
BsmAI GTCTC 2 cut(s) 27, 153
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 1 cut(s) 195
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 5 cut(s) 39, 92, 113, 276, 370
Bsp119I TTCGAA 1 cut(s) 326
Bsp143I GATC 4 cut(s) 4, 190, 267, 316
BspACI CCGC 3 cut(s) 73, 388, 496
BspANI GGCC 5 cut(s) 39, 92, 113, 276, 370
BspLI GGNNCC 1 cut(s) 211
BspPI GGATC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 326
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 4 cut(s) 4, 190, 267, 316
Bst2UI CCWGG 2 cut(s) 372, 398
Bst4CI ACNGT 3 cut(s) 250, 412, 508
BstBI TTCGAA 1 cut(s) 326
BstENI CCTNNNNNAGG 1 cut(s) 24
BstKTI GATC 4 cut(s) 7, 193, 270, 319
BstMAI GTCTC 2 cut(s) 27, 153
BstMBI GATC 4 cut(s) 4, 190, 267, 316
BstMCI CGRYCG 2 cut(s) 93, 414
BstMWI GCNNNNNNNGC 2 cut(s) 45, 89
BstNI CCWGG 2 cut(s) 372, 398
BstSCI CCNGG 2 cut(s) 370, 396
BstV1I GCAGC 2 cut(s) 92, 372
BstZI CGGCCG 1 cut(s) 90
BsuRI GGCC 5 cut(s) 39, 92, 113, 276, 370
BtgZI GCGATG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 472, 548
Cfr13I GGNCC 1 cut(s) 209
Csp6I GTAC 1 cut(s) 481
CspCI CAANNNNNGTGG 2 cut(s) 473, 508
CviAII CATG 4 cut(s) 241, 302, 320, 515
CviJI RGCY 8 cut(s) 39, 48, 83, 92, 113, 276, 370, 471
CviKI_1 RGCY 8 cut(s) 39, 48, 83, 92, 113, 276, 370, 471
CviQI GTAC 1 cut(s) 481
DpnI GATC 4 cut(s) 6, 192, 269, 318
DpnII GATC 4 cut(s) 4, 190, 267, 316
EaeI YGGCCR 2 cut(s) 37, 90
EagI CGGCCG 1 cut(s) 90
EciI GGCGGA 1 cut(s) 511
EclXI CGGCCG 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 209
Eco52I CGGCCG 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 448
EcoNI CCTNNNNNAGG 1 cut(s) 24
EcoRI GAATTC 1 cut(s) 296
EcoRII CCWGG 2 cut(s) 370, 396
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 4 cut(s) 244, 305, 323, 518
FaiI YATR 7 cut(s) 23, 116, 242, 303, 321, 383, 516
FaqI GGGAC 1 cut(s) 195
FatI CATG 4 cut(s) 240, 301, 319, 514
FauI CCCGC 1 cut(s) 381
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 2 cut(s) 81, 386
Fsp4HI GCNGC 2 cut(s) 81, 386
FspBI CTAG 2 cut(s) 194, 476
GluI GCNGC 2 cut(s) 81, 386
HaeIII GGCC 5 cut(s) 39, 92, 113, 276, 370
HapII CCGG 1 cut(s) 63
Hin1II CATG 4 cut(s) 244, 305, 323, 518
HinfI GANTC 2 cut(s) 11, 530
HpaII CCGG 1 cut(s) 63
Hpy166II GTNNAC 2 cut(s) 68, 307
Hpy188III TCNNGA 3 cut(s) 8, 265, 476
Hpy8I GTNNAC 2 cut(s) 68, 307
HpyAV CCTTC 3 cut(s) 70, 266, 472
HpyCH4III ACNGT 3 cut(s) 250, 412, 508
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 89
Hsp92II CATG 4 cut(s) 244, 305, 323, 518
Kzo9I GATC 4 cut(s) 4, 190, 267, 316
LmnI GCTCC 1 cut(s) 294
LpnPI CCDG 9 cut(s) 12, 76, 278, 357, 375, 383, 384, 410, 524
Lsp1109I GCAGC 2 cut(s) 92, 372
MaeI CTAG 2 cut(s) 194, 476
MaeIII GTNAC 1 cut(s) 250
MalI GATC 4 cut(s) 6, 192, 269, 318
MboI GATC 4 cut(s) 4, 190, 267, 316
MboII GAAGA 2 cut(s) 273, 276
MluCI AATT 3 cut(s) 58, 260, 296
MnlI CCTC 4 cut(s) 23, 88, 103, 483
MroXI GAANNNNTTC 2 cut(s) 15, 332
MspA1I CMGCKG 2 cut(s) 73, 83
MspI CCGG 1 cut(s) 63
MspR9I CCNGG 2 cut(s) 372, 398
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 2 cut(s) 372, 398
MwoI GCNNNNNNNGC 2 cut(s) 45, 89
NdeII GATC 4 cut(s) 4, 190, 267, 316
NlaIII CATG 4 cut(s) 244, 305, 323, 518
NlaIV GGNNCC 1 cut(s) 211
NmeAIII GCCGAG 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 250
NspV TTCGAA 1 cut(s) 326
PcsI WCGNNNNNNNCGW 1 cut(s) 179
PctI GAATGC 1 cut(s) 105
PdmI GAANNNNTTC 2 cut(s) 15, 332
PfeI GAWTC 2 cut(s) 11, 530
PkrI GCNGC 2 cut(s) 82, 387
Psp6I CCWGG 2 cut(s) 370, 396
PspGI CCWGG 2 cut(s) 370, 396
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
SatI GCNGC 2 cut(s) 81, 386
Sau3AI GATC 4 cut(s) 4, 190, 267, 316
Sau96I GGNCC 1 cut(s) 209
ScrFI CCNGG 2 cut(s) 372, 398
SetI ASST 5 cut(s) 50, 85, 129, 199, 494
SfuI TTCGAA 1 cut(s) 326
SinI GGWCC 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 150
SmoI CTYRAG 1 cut(s) 150
Sse9I AATT 3 cut(s) 58, 260, 296
SsiI CCGC 3 cut(s) 73, 388, 496
SspMI CTAG 2 cut(s) 194, 476
StyD4I CCNGG 2 cut(s) 370, 396
TaaI ACNGT 3 cut(s) 250, 412, 508
TaqI TCGA 4 cut(s) 135, 182, 270, 326
TasI AATT 3 cut(s) 58, 260, 296
TfiI GAWTC 2 cut(s) 11, 530
TscAI CASTG 2 cut(s) 472, 555
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 80, 385
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 211, 427
TspRI CASTG 2 cut(s) 472, 555
VpaK11BI GGWCC 1 cut(s) 209
XagI CCTNNNNNAGG 1 cut(s) 24
XapI RAATTY 3 cut(s) 58, 260, 296
XbaI TCTAGA 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 306
XmnI GAANNNNTTC 2 cut(s) 15, 332
XspI CTAG 2 cut(s) 194, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.