pycom15g04670

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001909
Physical Location & Seq
Reverse (-)
2767094 .. 2771462
4369 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2499 bp
ATGTTCCGAGCTGCTCCTGTGTGGTACGTTTTGACCTGCCAAAGACAGTGCGCAGTTATGTCCAGTCGAGGGGACGGGCTCGGCAAGATAACTCTCAGTTCATCACAATGCTGGAGAGGGGAAACAAGAAACAAAGGGATCGAACTATATGAGATTGTCAGGAGTGAGTTTTTGATGACAGATACAGCTATCAAAAGAGATCCTGAAGCATGCATTTTGAAAGCATGTCCTTTGGAGGAAACAAATATATATACTGTGGACTCCACTGGAGCATCAGTCACTGCAGATTCTAGTGTCAGTCTTGTACATAAATATTGTGAAACGCTGCCTAGGGATAAGTACTTCACTCCAAAGCCAACTTTTCATATCTCATACCTTGGAGAGTCTTATGAGTGTAAGATCACGTTGCCTCCAAATGCGGCTTTCCAAACGTTAGTTGGCCCTGTATGCAAGAAATCTTCTTTAGCAAAGCAGCTTGTCTGCTTGGAAGCGTGTCAGCAGCTACATCAAATGGGTGCCCTGAATGACCATCTTCTCCCAGTCATTGATGTGCCTGATGTGAAAAGAAAAGAGCAAGCTTCTGGTGCAGGAACTACTAAAAGGAAGGAACTACATGGTACAACTTGCATTCGTATGCTATCTGGAACTTGGGGGGAGAAACTTGATGCTGTCATTTTTCAGGGATATAAGTTTGACTTCTCCTGTAACATTGCCAGTGAGTTGTATTCTGGATTTGTTCTTCTCATTGAGTCGAAGCTTGCTGATGATGTGGGGAATCTCAAATTGGACCTTTACCTGATTTCGAAAACAGTCAAGGCTTCTGTTTCTTTCTGTGGGGAGGTTCATTTAGACGGGAAACAGATGGAAAAAGCAAAGTGCTTTCAGGAATTTTTCTTTAATGGATTGTTTGGGAAATTGTATGGAACCAAATCAGCTAGAAAACAGAGAGAACTTTCACTGGAGAAAGAAACAAGAAAACTGTGGACCCCATATTGCTTATATTTGCTTCTTCCAGTTGAGACATTGAATGATTCAAAAGATGAATCTTGGAGAATAAATTGGGCAGGGATCAATTCTTGTGTTTCTGTGGTAGATTTCTTAAAGAGAAACTCGTGTTTGAGCAGCCAGTGTAACGTTGACACAGGGAACTCAGTTCCAAGAACCGTCTCATCTGAGACTGAGTGCAACAGTGCAGGGATAGTTCAGTTTGCTAATTGCTCTGTTGATGTAAAAAATCTCAAAGATACGGTGGTGGTGGCTATCCATACTGGAAGAATCTACTCTGTTGATGAGGTTATGAGTAACACTTCTGCTGAGAGTCCTTTTGATGTAAAGAAAGACAATGTATCATCACAATATACTTCATTTTCTGAGTACTTCAACAAAAAATACGGGATTGTGCTCCGTTATCCTGGTCAGCCTCTGTTGCAACTGAAACAAAGTCATAATCCGCACAACCTTCTTGTGAACTTTAATGGTGAAGGTGGTGGTGGTAAGACATTGCAATCTGGCGTTGTTATTGGAAAGCAGATTATGTATGCTCACATGCCACCTGAGCTTCTAGTCGCTATTGGTATCCCGGGAGACGTTCTAAAGTCATGTTATTTACTACCATCGTTGATTCATCGCTTGGAGTCCTTGTTGCTAGCCAGTCAACTCAGAGAAGAGATTAATTATCATTCTAGCAGCTTTCGGATATCAAGCTTATTGATTCTAGAAGCACTTACAACACTAAGATGCAATGATGAATTTTCTATGGAGCGCTTGGAATTGCTCGGTGATTCAGTTTTAAAATATACTGTGAGCTGCCACCTCTTTCTCAAATATCCCAAAAAGCATGAAGGACAATTAACTAGTTGCCGCACCCTGGCTATTTGTAACTCAAACCTACATAGGTTGGGCATAAATCGCAAGTTACAGGGATACATCCGTGATTGTGCGTTTGACCCATGTCGTTGGGTTTCTCCAGGGCAGATTTCTCTACGACCCATTCCTTGTAATTGTGGAGTGGAGACTTTAGAAGTACCCGTAGATGGAAAATTCCAAACTGAAGACCCTAAAGTTGTGGTTGGAAAGAGCTGTGATAAGGGCCATAGATGGATGTGTTCCAAAACAATCTCTGATTGTGTTGAAGCCCTTATAGGAGCATACTATGTTGCTGGTGGATTGCCTGCTGCAATTCATATGATGAAGTGGCTCGATATTGATGTAGAACTTGAGCCTTCCTTGGTTGCTGATGCAATTACTATGTCGTCTCTTCATTCGTACACCCTGAAAGCTAATGAGATTGCAATCATGGAGTCAAAGCTTCAATACGAATTTTCTACCAAGGGTCTCTTACTCGAGGCCATAACACATGCATCTGATCAAGAATCAGGAGATGGCTACTGTTACGAGCGACTAGAATTTCTAGGTGACTCTGTGTTGGACCTGCTAATCACAAGGCATCTCTATCATAGCCACACAGATATCGATCCAAATCTGGGGCGAGTTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0031974 GO:0031981 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

833

Amino Acids

92.71

Weight (kDa)

6.53

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dicer_dimer PF03368 98 - 181 5.4e-26 Dicer dimerisation domain
PAZ PF02170 410 - 530 1.4e-13 PAZ domain
Ribonucleas_3_3 PF14622 586 - 644 5.7e-06 Ribonuclease-III-like
Ribonuclease_3 PF00636 587 - 722 7e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 778 - 822 3.5e-07 Ribonuclease-III-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 52
Acc36I ACCTGC 2 cut(s) 44, 2439
AccB1I GGYRCC 1 cut(s) 515
AciI CCGC 3 cut(s) 419, 1451, 1861
AclI AACGTT 2 cut(s) 431, 1134
AclWI GGATC 4 cut(s) 146, 194, 1076, 2468
AcsI RAATTY 5 cut(s) 887, 1748, 2039, 2318, 2405
AcuI CTGAAG 2 cut(s) 225, 2070
AfaI GTAC 7 cut(s) 26, 306, 341, 619, 1376, 2025, 2267
AfeI AGCGCT 1 cut(s) 1763
AfiI CCNNNNNNNGG 4 cut(s) 69, 1867, 2033, 2483
AgsI TTSAA 6 cut(s) 220, 1027, 1035, 1381, 2132, 2312
AhlI ACTAGT 1 cut(s) 1853
AjnI CCWGG 3 cut(s) 1411, 1866, 1966
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
Alw21I GWGCWC 1 cut(s) 1404
Alw26I GTCTC 7 cut(s) 1013, 1169, 1171, 1578, 2006, 2259, 2339
AlwI GGATC 4 cut(s) 146, 194, 1076, 2468
AlwNI CAGNNNCTG 2 cut(s) 281, 1423
Ama87I CYCGRG 2 cut(s) 1579, 2342
Aor51HI AGCGCT 1 cut(s) 1763
AoxI GGCC 3 cut(s) 439, 2089, 2346
ApeKI GCWGC 8 cut(s) 11, 325, 472, 499, 1122, 1686, 1806, 2174
ApoI RAATTY 5 cut(s) 887, 1748, 2039, 2318, 2405
AseI ATTAAT 1 cut(s) 1671
AspA2I CCTAGG 1 cut(s) 329
AspLEI GCGC 2 cut(s) 53, 1764
AspS9I GGNCC 5 cut(s) 440, 787, 984, 2089, 2428
AsuC2I CCSGG 2 cut(s) 1580, 1581
AsuHPI GGTGA 3 cut(s) 1490, 1790, 2426
AsuII TTCGAA 1 cut(s) 803
AsuNHI GCTAGC 1 cut(s) 1645
AvaI CYCGRG 2 cut(s) 1579, 2342
AvaII GGWCC 3 cut(s) 787, 984, 2428
AvrII CCTAGG 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 520
BaeI ACNNNNGTAYC 4 cut(s) 1236, 1269, 1915, 1948
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 81
BauI CACGAG 1 cut(s) 1111
BbsI GAAGAC 1 cut(s) 2058
Bbv12I GWGCWC 1 cut(s) 1404
BbvI GCAGC 7 cut(s) 312, 484, 511, 1134, 1698, 1793, 2161
BccI CCATC 6 cut(s) 537, 856, 1621, 2027, 2091, 2375
BciT130I CCWGG 3 cut(s) 1413, 1868, 1968
BciVI GTATCC 2 cut(s) 1586, 1916
BclI TGATCA 1 cut(s) 2365
BcnI CCSGG 2 cut(s) 1580, 1581
BcoDI GTCTC 7 cut(s) 1013, 1169, 1171, 1578, 2006, 2259, 2339
BcuI ACTAGT 1 cut(s) 1853
BfmI CTRYAG 1 cut(s) 282
BfoI RGCGCY 1 cut(s) 1765
BfuAI ACCTGC 2 cut(s) 44, 2439
BfuI GTATCC 2 cut(s) 1586, 1916
BlnI CCTAGG 1 cut(s) 329
BmcAI AGTACT 2 cut(s) 341, 1376
Bme1390I CCNGG 5 cut(s) 1413, 1580, 1581, 1868, 1968
Bme18I GGWCC 3 cut(s) 787, 984, 2428
BmeT110I CYCGRG 2 cut(s) 1579, 2342
BmgT120I GGNCC 5 cut(s) 440, 787, 984, 2089, 2428
BmiI GGNNCC 3 cut(s) 517, 925, 986
BmrFI CCNGG 5 cut(s) 1413, 1580, 1581, 1868, 1968
BmrI ACTGGG 1 cut(s) 533
BmsI GCATC 6 cut(s) 281, 655, 1727, 2227, 2369, 2455
BmtI GCTAGC 1 cut(s) 1649
BmuI ACTGGG 1 cut(s) 533
BoxI GACNNNNGTC 1 cut(s) 1950
BpiI GAAGAC 1 cut(s) 2058
BpmI CTGGAG 4 cut(s) 133, 288, 980, 1950
Bpu10I CCTNAGC 1 cut(s) 1554
Bpu14I TTCGAA 1 cut(s) 803
BpuEI CTTGAG 1 cut(s) 2237
BpuMI CCSGG 2 cut(s) 1580, 1581
Bsa29I ATCGAT 1 cut(s) 2472
BsaBI GATNNNNATC 3 cut(s) 2291, 2472, 2478
BsaI GGTCTC 1 cut(s) 2339
BsaJI CCNNGG 7 cut(s) 329, 376, 1579, 1866, 1967, 2226, 2328
BsaXI ACNNNNNCTCC 6 cut(s) 261, 291, 394, 424, 1625, 1655
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 1867, 2033, 2483
Bse1I ACTGG 9 cut(s) 63, 271, 539, 714, 963, 1013, 1126, 1273, 1650
Bse3DI GCAATG 3 cut(s) 708, 1499, 1747
Bse8I GATNNNNATC 3 cut(s) 2291, 2472, 2478
BseBI CCWGG 3 cut(s) 1413, 1868, 1968
BseCI ATCGAT 1 cut(s) 2472
BseDI CCNNGG 7 cut(s) 329, 376, 1579, 1866, 1967, 2226, 2328
BseGI GGATG 2 cut(s) 1926, 2106
BseJI GATNNNNATC 3 cut(s) 2291, 2472, 2478
BseLI CCNNNNNNNGG 4 cut(s) 69, 1867, 2033, 2483
BseMI GCAATG 3 cut(s) 708, 1499, 1747
BseMII CTCAG 8 cut(s) 109, 1164, 1164, 1170, 1305, 1362, 1545, 1672
BseNI ACTGG 9 cut(s) 63, 271, 539, 714, 963, 1013, 1126, 1273, 1650
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 7 cut(s) 312, 484, 511, 1134, 1698, 1793, 2161
BsgI GTGCAG 2 cut(s) 606, 1212
BshFI GGCC 3 cut(s) 441, 2091, 2348
BshNI GGYRCC 1 cut(s) 515
BshVI ATCGAT 1 cut(s) 2472
BsiHKAI GWGCWC 1 cut(s) 1404
BsiHKCI CYCGRG 2 cut(s) 1579, 2342
BsiSI CCGG 1 cut(s) 1580
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 4 cut(s) 69, 1867, 2033, 2483
BsmAI GTCTC 7 cut(s) 1013, 1169, 1171, 1578, 2006, 2259, 2339
BsmBI CGTCTC 3 cut(s) 1171, 1578, 2259
BsmFI GGGAC 1 cut(s) 86
BsmI GAATGC 1 cut(s) 627
BsnI GGCC 3 cut(s) 441, 2091, 2348
Bso31I GGTCTC 1 cut(s) 2339
BsoBI CYCGRG 2 cut(s) 1579, 2342
Bsp119I TTCGAA 1 cut(s) 803
Bsp1286I GDGCHC 3 cut(s) 81, 520, 1404
Bsp1407I TGTACA 1 cut(s) 304
Bsp143I GATC 6 cut(s) 138, 199, 399, 1068, 2365, 2473
BspACI CCGC 3 cut(s) 419, 1451, 1861
BspANI GGCC 3 cut(s) 441, 2091, 2348
BspCNI CTCAG 8 cut(s) 108, 1163, 1165, 1171, 1306, 1363, 1546, 1671
BspDI ATCGAT 1 cut(s) 2472
BspLI GGNNCC 3 cut(s) 517, 925, 986
BspMAI CTGCAG 1 cut(s) 286
BspMI ACCTGC 2 cut(s) 44, 2439
BspOI GCTAGC 1 cut(s) 1649
BspPI GGATC 4 cut(s) 146, 194, 1076, 2468
BspT104I TTCGAA 1 cut(s) 803
BspT107I GGYRCC 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 2339
BsrDI GCAATG 3 cut(s) 708, 1499, 1747
BsrGI TGTACA 1 cut(s) 304
BsrI ACTGG 9 cut(s) 63, 271, 539, 714, 963, 1013, 1126, 1273, 1650
BssECI CCNNGG 7 cut(s) 329, 376, 1579, 1866, 1967, 2226, 2328
BssMI GATC 6 cut(s) 138, 199, 399, 1068, 2365, 2473
BssSI CACGAG 1 cut(s) 1111
BssT1I CCWWGG 4 cut(s) 329, 376, 2226, 2328
Bst2BI CACGAG 1 cut(s) 1111
Bst2UI CCWGG 3 cut(s) 1413, 1868, 1968
Bst4CI ACNGT 9 cut(s) 48, 256, 811, 981, 1165, 1190, 1249, 1801, 2390
Bst6I CTCTTC 2 cut(s) 1659, 2262
BstAUI TGTACA 1 cut(s) 304
BstBI TTCGAA 1 cut(s) 803
BstC8I GCNNGC 5 cut(s) 211, 576, 759, 1647, 2172
BstDEI CTNAG 9 cut(s) 95, 1150, 1173, 1179, 1314, 1371, 1554, 1658, 1733
BstF5I GGATG 2 cut(s) 1926, 2106
BstH2I RGCGCY 1 cut(s) 1765
BstHHI GCGC 2 cut(s) 53, 1764
BstKTI GATC 6 cut(s) 141, 202, 402, 1071, 2368, 2476
BstMAI GTCTC 7 cut(s) 1013, 1169, 1171, 1578, 2006, 2259, 2339
BstMBI GATC 6 cut(s) 138, 199, 399, 1068, 2365, 2473
BstMWI GCNNNNNNNGC 5 cut(s) 447, 584, 1426, 1555, 1908
BstNI CCWGG 3 cut(s) 1413, 1868, 1968
BstNSI RCATGY 4 cut(s) 213, 228, 1549, 2360
BstPAI GACNNNNGTC 1 cut(s) 1950
BstSCI CCNGG 5 cut(s) 1411, 1578, 1579, 1866, 1966
BstSFI CTRYAG 1 cut(s) 282
BstSLI GKGCMC 1 cut(s) 520
BstV1I GCAGC 7 cut(s) 312, 484, 511, 1134, 1698, 1793, 2161
BstV2I GAAGAC 1 cut(s) 2058
BstX2I RGATCY 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 1956
BstYI RGATCY 1 cut(s) 199
Bsu15I ATCGAT 1 cut(s) 2472
BsuI GTATCC 2 cut(s) 1586, 1916
BsuRI GGCC 3 cut(s) 441, 2091, 2348
BsuTUI ATCGAT 1 cut(s) 2472
BtgZI GCGATG 1 cut(s) 1610
BtsCI GGATG 2 cut(s) 1926, 2106
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 7 cut(s) 53, 264, 279, 721, 956, 1133, 1195
BveI ACCTGC 2 cut(s) 44, 2439
Cac8I GCNNGC 5 cut(s) 211, 576, 759, 1647, 2172
CaiI CAGNNNCTG 2 cut(s) 281, 1423
CfoI GCGC 2 cut(s) 53, 1764
Cfr13I GGNCC 5 cut(s) 440, 787, 984, 2089, 2428
Cfr9I CCCGGG 1 cut(s) 1579
ClaI ATCGAT 1 cut(s) 2472
Csp6I GTAC 7 cut(s) 25, 305, 340, 618, 1375, 2024, 2266
CviAII CATG 9 cut(s) 210, 225, 614, 1546, 1599, 1838, 1950, 2296, 2357
CviQI GTAC 7 cut(s) 25, 305, 340, 618, 1375, 2024, 2266
DdeI CTNAG 9 cut(s) 95, 1150, 1173, 1179, 1314, 1371, 1554, 1658, 1733
DpnI GATC 6 cut(s) 140, 201, 401, 1070, 2367, 2475
DpnII GATC 6 cut(s) 138, 199, 399, 1068, 2365, 2473
DraI TTTAAA 1 cut(s) 1791
Eam1104I CTCTTC 2 cut(s) 1659, 2262
EarI CTCTTC 2 cut(s) 1659, 2262
Eco130I CCWWGG 4 cut(s) 329, 376, 2226, 2328
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 2339
Eco32I GATATC 2 cut(s) 1698, 2470
Eco47I GGWCC 3 cut(s) 787, 984, 2428
Eco47III AGCGCT 1 cut(s) 1763
Eco57I CTGAAG 2 cut(s) 225, 2070
Eco88I CYCGRG 2 cut(s) 1579, 2342
EcoRII CCWGG 3 cut(s) 1411, 1866, 1966
EcoRV GATATC 2 cut(s) 1698, 2470
EcoT14I CCWWGG 4 cut(s) 329, 376, 2226, 2328
EcoT22I ATGCAT 2 cut(s) 215, 2362
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 4 cut(s) 329, 376, 2226, 2328
Esp3I CGTCTC 3 cut(s) 1171, 1578, 2259
FaeI CATG 9 cut(s) 213, 228, 617, 1549, 1602, 1841, 1953, 2299, 2360
FalI AAGNNNNNCTT 4 cut(s) 680, 712, 2321, 2353
FaqI GGGAC 1 cut(s) 86
FatI CATG 9 cut(s) 209, 224, 613, 1545, 1598, 1837, 1949, 2295, 2356
FauNDI CATATG 1 cut(s) 2184
FbaI TGATCA 1 cut(s) 2365
FokI GGATG 2 cut(s) 1913, 2113
FriOI GRGCYC 1 cut(s) 81
FspI TGCGCA 1 cut(s) 52
GlaI GCGC 2 cut(s) 52, 1763
GsuI CTGGAG 4 cut(s) 133, 288, 980, 1950
HaeII RGCGCY 1 cut(s) 1765
HaeIII GGCC 3 cut(s) 441, 2091, 2348
HapII CCGG 1 cut(s) 1580
HhaI GCGC 2 cut(s) 53, 1764
Hin1II CATG 9 cut(s) 213, 228, 617, 1549, 1602, 1841, 1953, 2299, 2360
Hin6I GCGC 2 cut(s) 51, 1762
HinP1I GCGC 2 cut(s) 51, 1762
HincII GTYRAC 3 cut(s) 1138, 1655, 2494
HindII GTYRAC 3 cut(s) 1138, 1655, 2494
HindIII AAGCTT 4 cut(s) 576, 755, 1702, 2306
HpaI GTTAAC 1 cut(s) 2494
HpaII CCGG 1 cut(s) 1580
HphI GGTGA 3 cut(s) 1490, 1790, 2426
Hpy166II GTNNAC 7 cut(s) 259, 984, 1138, 1468, 1655, 2268, 2494
Hpy188I TCNGA 7 cut(s) 8, 1174, 1372, 1661, 1695, 2122, 2365
Hpy188III TCNNGA 8 cut(s) 160, 203, 642, 729, 884, 1715, 2369, 2376
Hpy8I GTNNAC 7 cut(s) 259, 984, 1138, 1468, 1655, 2268, 2494
HpyAV CCTTC 5 cut(s) 598, 1469, 1475, 1835, 2232
HpyCH4III ACNGT 9 cut(s) 48, 256, 811, 981, 1165, 1190, 1249, 1801, 2390
HpyCH4IV ACGT 5 cut(s) 27, 404, 431, 1134, 1587
HpyF10VI GCNNNNNNNGC 5 cut(s) 447, 584, 1426, 1555, 1908
HpyF3I CTNAG 9 cut(s) 95, 1150, 1173, 1179, 1314, 1371, 1554, 1658, 1733
HpySE526I ACGT 5 cut(s) 27, 404, 431, 1134, 1587
Hsp92II CATG 9 cut(s) 213, 228, 617, 1549, 1602, 1841, 1953, 2299, 2360
HspAI GCGC 2 cut(s) 51, 1762
Ksp22I TGATCA 1 cut(s) 2365
KspAI GTTAAC 1 cut(s) 2494
Kzo9I GATC 6 cut(s) 138, 199, 399, 1068, 2365, 2473
LmnI GCTCC 5 cut(s) 19, 269, 1407, 1759, 2144
Lsp1109I GCAGC 7 cut(s) 312, 484, 511, 1134, 1698, 1793, 2161
LweI GCATC 6 cut(s) 281, 655, 1727, 2227, 2369, 2455
MaeII ACGT 5 cut(s) 27, 404, 431, 1134, 1587
MaeIII GTNAC 8 cut(s) 277, 704, 1130, 1301, 1877, 1914, 2390, 2414
MalI GATC 6 cut(s) 140, 201, 401, 1070, 2367, 2475
MboI GATC 6 cut(s) 138, 199, 399, 1068, 2365, 2473
MboII GAAGA 8 cut(s) 450, 524, 731, 1001, 1284, 1676, 2063, 2249
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 3 cut(s) 81, 520, 1404
MlyI GAGTC 7 cut(s) 254, 392, 758, 1327, 1643, 2309, 2411
MmeI TCCRAC 2 cut(s) 2050, 2406
MnlI CCTC 9 cut(s) 62, 110, 229, 420, 832, 1285, 1431, 1823, 2338
Mph1103I ATGCAT 2 cut(s) 215, 2362
MseI TTAA 7 cut(s) 897, 1100, 1473, 1671, 1790, 1850, 2493
MslI CAYNNNNRTG 4 cut(s) 106, 548, 632, 1735
MspI CCGG 1 cut(s) 1580
MspR9I CCNGG 5 cut(s) 1413, 1580, 1581, 1868, 1968
Mva1269I GAATGC 1 cut(s) 627
MvaI CCWGG 3 cut(s) 1413, 1868, 1968
MwoI GCNNNNNNNGC 5 cut(s) 447, 584, 1426, 1555, 1908
NciI CCSGG 2 cut(s) 1580, 1581
NdeI CATATG 1 cut(s) 2184
NdeII GATC 6 cut(s) 138, 199, 399, 1068, 2365, 2473
NheI GCTAGC 1 cut(s) 1645
NlaIII CATG 9 cut(s) 213, 228, 617, 1549, 1602, 1841, 1953, 2299, 2360
NlaIV GGNNCC 3 cut(s) 517, 925, 986
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 2 cut(s) 277, 2414
NsbI TGCGCA 1 cut(s) 52
NsiI ATGCAT 2 cut(s) 215, 2362
NspI RCATGY 4 cut(s) 213, 228, 1549, 2360
NspV TTCGAA 1 cut(s) 803
PaeI GCATGC 1 cut(s) 213
PaeR7I CTCGAG 1 cut(s) 2342
PctI GAATGC 1 cut(s) 627
PfeI GAWTC 9 cut(s) 287, 775, 1031, 1043, 1275, 1621, 1711, 1781, 2372
PleI GAGTC 7 cut(s) 254, 391, 757, 1326, 1642, 2308, 2411
PpsI GAGTC 7 cut(s) 254, 391, 757, 1326, 1642, 2308, 2411
PshAI GACNNNNGTC 1 cut(s) 1950
PshBI ATTAAT 1 cut(s) 1671
Psp1406I AACGTT 2 cut(s) 431, 1134
Psp6I CCWGG 3 cut(s) 1411, 1866, 1966
PspGI CCWGG 3 cut(s) 1411, 1866, 1966
PspN4I GGNNCC 3 cut(s) 517, 925, 986
PspPI GGNCC 5 cut(s) 440, 787, 984, 2089, 2428
PspXI VCTCGAGB 1 cut(s) 2342
PstI CTGCAG 1 cut(s) 286
PstNI CAGNNNCTG 2 cut(s) 281, 1423
PsuI RGATCY 1 cut(s) 199
RsaI GTAC 7 cut(s) 26, 306, 341, 619, 1376, 2025, 2267
RsaNI GTAC 7 cut(s) 25, 305, 340, 618, 1375, 2024, 2266
RseI CAYNNNNRTG 4 cut(s) 106, 548, 632, 1735
SaqAI TTAA 7 cut(s) 897, 1100, 1473, 1671, 1790, 1850, 2493
Sau3AI GATC 6 cut(s) 138, 199, 399, 1068, 2365, 2473
Sau96I GGNCC 5 cut(s) 440, 787, 984, 2089, 2428
ScaI AGTACT 2 cut(s) 341, 1376
SchI GAGTC 7 cut(s) 254, 392, 758, 1327, 1643, 2309, 2411
ScrFI CCNGG 5 cut(s) 1413, 1580, 1581, 1868, 1968
SduI GDGCHC 3 cut(s) 81, 520, 1404
SfaNI GCATC 6 cut(s) 281, 655, 1727, 2227, 2369, 2455
SfcI CTRYAG 1 cut(s) 282
Sfr274I CTCGAG 1 cut(s) 2342
SfuI TTCGAA 1 cut(s) 803
SinI GGWCC 3 cut(s) 787, 984, 2428
SlaI CTCGAG 1 cut(s) 2342
SmaI CCCGGG 1 cut(s) 1581
SmiMI CAYNNNNRTG 4 cut(s) 106, 548, 632, 1735
SmlI CTYRAG 2 cut(s) 2216, 2342
SmoI CTYRAG 2 cut(s) 2216, 2342
SpeI ACTAGT 1 cut(s) 1853
SphI GCATGC 1 cut(s) 213
SsiI CCGC 3 cut(s) 419, 1451, 1861
SspI AATATT 1 cut(s) 314
StyD4I CCNGG 5 cut(s) 1411, 1578, 1579, 1866, 1966
StyI CCWWGG 4 cut(s) 329, 376, 2226, 2328
TaaI ACNGT 9 cut(s) 48, 256, 811, 981, 1165, 1190, 1249, 1801, 2390
TaiI ACGT 5 cut(s) 30, 407, 434, 1137, 1590
TaqI TCGA 7 cut(s) 67, 141, 752, 803, 2199, 2343, 2472
TatI WGTACW 3 cut(s) 304, 339, 1374
TauI GCSGC 2 cut(s) 422, 1863
TfiI GAWTC 9 cut(s) 287, 775, 1031, 1043, 1275, 1621, 1711, 1781, 2372
Tru1I TTAA 7 cut(s) 897, 1100, 1473, 1671, 1790, 1850, 2493
Tru9I TTAA 7 cut(s) 897, 1100, 1473, 1671, 1790, 1850, 2493
TscAI CASTG 7 cut(s) 53, 271, 286, 721, 963, 1133, 1195
TseFI GTSAC 2 cut(s) 277, 2414
TseI GCWGC 8 cut(s) 11, 325, 472, 499, 1122, 1686, 1806, 2174
Tsp45I GTSAC 2 cut(s) 277, 2414
TspGWI ACGGA 2 cut(s) 1394, 1919
TspMI CCCGGG 1 cut(s) 1579
TspRI CASTG 7 cut(s) 53, 271, 286, 721, 963, 1133, 1195
VpaK11BI GGWCC 3 cut(s) 787, 984, 2428
VspI ATTAAT 1 cut(s) 1671
XapI RAATTY 5 cut(s) 887, 1748, 2039, 2318, 2405
XbaI TCTAGA 1 cut(s) 1714
XceI RCATGY 4 cut(s) 213, 228, 1549, 2360
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XhoI CTCGAG 1 cut(s) 2342
XmaI CCCGGG 1 cut(s) 1579
XmaJI CCTAGG 1 cut(s) 329
ZrmI AGTACT 2 cut(s) 341, 1376
Zsp2I ATGCAT 2 cut(s) 215, 2362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.