pycom15g06430

NADH dehydrogenase ubiquinone iron-sulfur protein 4, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002607
Physical Location & Seq
Reverse (-)
3938265 .. 3939786
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGGCGCGACCTCTACGACACGGCTTCAACATCGCCCGAGCGCACGTGCTTTCGCGGCCGCGATGGGCGTCGTCGCAGTCAGCGGAGGCACTGGTGGAGGTCAAAGTCCCCGACATAGGCATGATATCCGGAATCCCCGAACAGCATCTCCGACGAAGGGTTATAATCTATTCGCCTGCTCGAACTGCGACCCAACAAGGTTCTGGGAAAGTCGGGAAATGGAAGATTAATTTTCTTTCGACGCAAAAGTGGGAAAACCCATTGATGGGCTGGACATCTACAGGTGATCCGTACGCTAATGTTGGTGAGGCAGGACTGACTTTTGAGAGTGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGCTGGGATTATACGGTCAGGAAGCGTCACACACCACTTTTGAAGGCTAAGGCATATGCAGACAACTTCAAGTGGAAGGGCCCTGATAAGAGCGAGAAGACTGCAGCTGATGCTGCAAACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
ETC_C1_NDUFA4

Protein Analysis

163

Amino Acids

18.06

Weight (kDa)

9.89

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUS4 PF04800 54 - 147 8.3e-32 NADH dehydrogenase ubiquinone Fe-S protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 164
AccII CGCG 3 cut(s) 7, 55, 61
AccIII TCCGGA 1 cut(s) 128
AciI CCGC 3 cut(s) 55, 59, 83
AclWI GGATC 1 cut(s) 281
AcoI YGGCCR 1 cut(s) 56
AcvI CACGTG 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 68
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 4 cut(s) 116, 157, 265, 266
AgsI TTSAA 3 cut(s) 28, 406, 433
AloI GAACNNNNNNTCC 2 cut(s) 132, 164
AluBI AGCT 2 cut(s) 338, 470
AluI AGCT 2 cut(s) 338, 470
AlwI GGATC 1 cut(s) 281
Ama87I CYCGRG 1 cut(s) 36
Aor13HI TCCGGA 1 cut(s) 128
AoxI GGCC 2 cut(s) 56, 442
ApaI GGGCCC 1 cut(s) 446
ApeKI GCWGC 3 cut(s) 338, 467, 476
AseI ATTAAT 1 cut(s) 228
AspLEI GCGC 2 cut(s) 7, 43
AspS9I GGNCC 2 cut(s) 442, 443
AsuHPI GGTGA 2 cut(s) 296, 317
AvaI CYCGRG 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 446
BbrPI CACGTG 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 467
BbvI GCAGC 3 cut(s) 325, 463, 479
BccI CCATC 2 cut(s) 57, 259
BceAI ACGGC 1 cut(s) 37
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BfmI CTRYAG 2 cut(s) 279, 465
BisI GCNGC 5 cut(s) 56, 59, 339, 468, 477
BlsI GCNGC 5 cut(s) 57, 60, 340, 469, 478
BmeT110I CYCGRG 1 cut(s) 36
BmgT120I GGNCC 2 cut(s) 442, 443
BmiI GGNNCC 1 cut(s) 444
BmsI GCATC 2 cut(s) 154, 463
BpiI GAAGAC 1 cut(s) 467
Bpu10I CCTNAGC 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 46
BsaHI GRCGYC 1 cut(s) 68
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 4 cut(s) 116, 157, 265, 266
Bse1I ACTGG 1 cut(s) 96
BseAI TCCGGA 1 cut(s) 128
BseLI CCNNNNNNNGG 4 cut(s) 116, 157, 265, 266
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 446
BseX3I CGGCCG 1 cut(s) 56
BseXI GCAGC 3 cut(s) 325, 463, 479
BseYI CCCAGC 1 cut(s) 366
Bsh1236I CGCG 3 cut(s) 7, 55, 61
Bsh1285I CGRYCG 1 cut(s) 59
BshFI GGCC 2 cut(s) 58, 444
BsiEI CGRYCG 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 1 cut(s) 129
BsiWI CGTACG 1 cut(s) 291
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 4 cut(s) 116, 157, 265, 266
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 2 cut(s) 58, 444
BsoBI CYCGRG 1 cut(s) 36
Bsp120I GGGCCC 1 cut(s) 442
Bsp1286I GDGCHC 1 cut(s) 446
Bsp13I TCCGGA 1 cut(s) 128
Bsp143I GATC 1 cut(s) 286
BspACI CCGC 3 cut(s) 55, 59, 83
BspANI GGCC 2 cut(s) 58, 444
BspCNI CTCAG 1 cut(s) 346
BspEI TCCGGA 1 cut(s) 128
BspFNI CGCG 3 cut(s) 7, 55, 61
BspLI GGNNCC 1 cut(s) 444
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 1 cut(s) 281
BsrI ACTGG 1 cut(s) 96
BssMI GATC 1 cut(s) 286
BssNI GRCGYC 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 379
BstACI GRCGYC 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 473
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 2 cut(s) 354, 411
BstFNI CGCG 3 cut(s) 7, 55, 61
BstHHI GCGC 2 cut(s) 7, 43
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstMCI CGRYCG 1 cut(s) 59
BstMWI GCNNNNNNNGC 5 cut(s) 55, 185, 344, 473, 476
BstSFI CTRYAG 2 cut(s) 279, 465
BstSLI GKGCMC 1 cut(s) 446
BstUI CGCG 3 cut(s) 7, 55, 61
BstV1I GCAGC 3 cut(s) 325, 463, 479
BstV2I GAAGAC 1 cut(s) 467
BstZI CGGCCG 1 cut(s) 56
BsuRI GGCC 2 cut(s) 58, 444
BtgZI GCGATG 2 cut(s) 16, 76
BtsIMutI CAGTG 1 cut(s) 89
Cac8I GCNNGC 1 cut(s) 177
CciNI GCGGCCGC 1 cut(s) 56
CfoI GCGC 2 cut(s) 7, 43
Cfr13I GGNCC 2 cut(s) 442, 443
CseI GACGC 3 cut(s) 57, 250, 377
Csp6I GTAC 1 cut(s) 292
CviAII CATG 2 cut(s) 121, 362
CviJI RGCY 8 cut(s) 24, 58, 270, 338, 366, 410, 444, 470
CviKI_1 RGCY 8 cut(s) 24, 58, 270, 338, 366, 410, 444, 470
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 2 cut(s) 354, 411
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 56
EagI CGGCCG 1 cut(s) 56
EclXI CGGCCG 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 446
Eco32I GATATC 1 cut(s) 126
Eco52I CGGCCG 1 cut(s) 56
Eco72I CACGTG 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 36
EcoO109I RGGNCCY 2 cut(s) 442, 443
EcoRV GATATC 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 446
FaeI CATG 2 cut(s) 124, 365
FaiI YATR 7 cut(s) 116, 122, 164, 363, 375, 418, 420
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 120, 361
FauNDI CATATG 1 cut(s) 418
Fnu4HI GCNGC 5 cut(s) 56, 59, 339, 468, 477
FriOI GRGCYC 1 cut(s) 446
Fsp4HI GCNGC 5 cut(s) 56, 59, 339, 468, 477
GlaI GCGC 2 cut(s) 6, 42
GluI GCNGC 5 cut(s) 56, 59, 339, 468, 477
GsaI CCCAGC 1 cut(s) 370
HaeIII GGCC 2 cut(s) 58, 444
HapII CCGG 1 cut(s) 129
HgaI GACGC 3 cut(s) 57, 250, 377
HhaI GCGC 2 cut(s) 7, 43
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 2 cut(s) 124, 365
Hin6I GCGC 2 cut(s) 5, 41
HinP1I GCGC 2 cut(s) 5, 41
HinfI GANTC 1 cut(s) 132
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 296, 317
Hpy188I TCNGA 2 cut(s) 152, 488
Hpy188III TCNNGA 3 cut(s) 129, 214, 382
Hpy99I CGWCG 4 cut(s) 73, 76, 156, 244
HpyAV CCTTC 3 cut(s) 150, 400, 433
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 4 cut(s) 341, 422, 467, 479
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 185, 344, 473, 476
HpyF3I CTNAG 2 cut(s) 354, 411
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 2 cut(s) 124, 365
HspAI GCGC 2 cut(s) 5, 41
Kpn2I TCCGGA 1 cut(s) 128
Kzo9I GATC 1 cut(s) 286
Lsp1109I GCAGC 3 cut(s) 325, 463, 479
LweI GCATC 2 cut(s) 154, 463
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 389
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 3 cut(s) 235, 344, 472
MhlI GDGCHC 1 cut(s) 446
MluCI AATT 1 cut(s) 229
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 4 cut(s) 21, 79, 91, 301
MroI TCCGGA 1 cut(s) 128
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 2 cut(s) 83, 470
MspI CCGG 1 cut(s) 129
MvnI CGCG 3 cut(s) 7, 55, 61
MwoI GCNNNNNNNGC 5 cut(s) 55, 185, 344, 473, 476
NdeI CATATG 1 cut(s) 418
NdeII GATC 1 cut(s) 286
NlaIII CATG 2 cut(s) 124, 365
NlaIV GGNNCC 1 cut(s) 444
NmuCI GTSAC 1 cut(s) 389
NotI GCGGCCGC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 132
Pfl23II CGTACG 1 cut(s) 291
PkrI GCNGC 5 cut(s) 57, 60, 340, 469, 478
PmaCI CACGTG 1 cut(s) 46
PmlI CACGTG 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 46
PshBI ATTAAT 1 cut(s) 228
PsiI TTATAA 1 cut(s) 164
PspCI CACGTG 1 cut(s) 46
PspFI CCCAGC 1 cut(s) 366
PspLI CGTACG 1 cut(s) 291
PspN4I GGNNCC 1 cut(s) 444
PspOMI GGGCCC 1 cut(s) 442
PspPI GGNCC 2 cut(s) 442, 443
PstI CTGCAG 1 cut(s) 469
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 5 cut(s) 56, 59, 339, 468, 477
Sau3AI GATC 1 cut(s) 286
Sau96I GGNCC 2 cut(s) 442, 443
SduI GDGCHC 1 cut(s) 446
SetI ASST 7 cut(s) 13, 48, 102, 202, 286, 340, 472
SfaNI GCATC 2 cut(s) 154, 463
SfcI CTRYAG 2 cut(s) 279, 465
SmiMI CAYNNNNRTG 1 cut(s) 119
Sse9I AATT 1 cut(s) 229
SsiI CCGC 3 cut(s) 55, 59, 83
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 1 cut(s) 48
TaqI TCGA 2 cut(s) 181, 239
TasI AATT 1 cut(s) 229
TauI GCSGC 2 cut(s) 58, 61
TfiI GAWTC 1 cut(s) 132
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 1 cut(s) 96
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 3 cut(s) 338, 467, 476
Tsp45I GTSAC 1 cut(s) 389
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 1 cut(s) 96
VspI ATTAAT 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 2 cut(s) 200, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.