pycom15g09890

glyoxalase I family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003849
Physical Location & Seq
Reverse (-)
6431474 .. 6433000
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 555 bp
ATGTCTTGCCAAATACTATTTGCTGGCAATTGCGTCATGGGCATTTCATATGTAATAAATTTCTCATATTTATTACATAGAAAGGGCAAAAAAACTTGTCATGGTTTCAAAAAGAGGAGAGGAGATGCCAAAACAAATATAGACAAAGTTTTAATAGTCAGTTTCCCTGGGTCAGCACACAGCCTAGAAAGCGAGGGAAAAATGGCAGCGGCGTCGTTTAGGTGGCTACTGCAGCTGCACAAGGACGTGCCAAAAGCCGCTCGGTTCTACGCCGAAGGGTTGGACTTCACCGTCAATGTCTGCACTCTTCGATGGGCGGAGCTCCAGTCCGGCCCGCTCAAGCTTTCCCTCATGCAATCCCCCAAGGATCATGTCATGCAGAGCGGAAACTCTTCACTGTTGTCCTTCACGGTGCCAGACATTAATCAAACGGTGACCAAGTTGATGGCGTTGGGAGCTGAACTCGATGGCCCTATCAAATATGAAGTTCACGGCAAGGTTGCAGCCATGCGATGTATTGACGGGCACATGCTAGGCCTCTATGAACCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

185

Amino Acids

20.29

Weight (kDa)

9.22

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 78 - 169 1e-05 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 290
AccB1I GGYRCC 1 cut(s) 412
AccBSI CCGCTC 3 cut(s) 260, 337, 384
AciI CCGC 5 cut(s) 209, 258, 317, 335, 384
AclWI GGATC 1 cut(s) 375
AcsI RAATTY 1 cut(s) 58
AcyI GRCGYC 1 cut(s) 212
AgsI TTSAA 1 cut(s) 109
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 4 cut(s) 235, 322, 343, 458
AluI AGCT 4 cut(s) 235, 322, 343, 458
Alw21I GWGCWC 1 cut(s) 324
AlwI GGATC 1 cut(s) 375
AoxI GGCC 3 cut(s) 331, 469, 535
ApeKI GCWGC 4 cut(s) 206, 232, 235, 503
ApoI RAATTY 1 cut(s) 58
AseI ATTAAT 1 cut(s) 423
Asp700I GAANNNNTTC 1 cut(s) 391
AspS9I GGNCC 2 cut(s) 332, 470
AsuHPI GGTGA 2 cut(s) 280, 445
BaeGI GKGCMC 1 cut(s) 528
BanI GGYRCC 1 cut(s) 412
BanII GRGCYC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 4 cut(s) 218, 222, 244, 515
BccI CCATC 3 cut(s) 306, 439, 461
BceAI ACGGC 1 cut(s) 508
BcgI CGANNNNNNTGC 2 cut(s) 195, 229
BciT130I CCWGG 1 cut(s) 168
BfaI CTAG 3 cut(s) 185, 533, 553
BfmI CTRYAG 1 cut(s) 230
BisI GCNGC 6 cut(s) 207, 210, 233, 236, 258, 504
BlsI GCNGC 6 cut(s) 208, 211, 234, 237, 259, 505
Bme1390I CCNGG 1 cut(s) 168
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 2 cut(s) 332, 470
BmiI GGNNCC 1 cut(s) 414
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 115
BplI GAGNNNNNCTC 2 cut(s) 447, 479
BpmI CTGGAG 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 323
BsaHI GRCGYC 1 cut(s) 212
BsaJI CCNNGG 3 cut(s) 166, 167, 363
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bse1I ACTGG 1 cut(s) 325
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 3 cut(s) 166, 167, 363
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 2 cut(s) 130, 135
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 4 cut(s) 218, 222, 244, 515
BsgI GTGCAG 2 cut(s) 221, 286
BshFI GGCC 3 cut(s) 333, 471, 537
BshNI GGYRCC 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 324
BsiSI CCGG 1 cut(s) 330
BsnI GGCC 3 cut(s) 333, 471, 537
Bsp1286I GDGCHC 2 cut(s) 324, 528
Bsp143I GATC 1 cut(s) 367
BspACI CCGC 5 cut(s) 209, 258, 317, 335, 384
BspANI GGCC 3 cut(s) 333, 471, 537
BspLI GGNNCC 1 cut(s) 414
BspMAI CTGCAG 1 cut(s) 234
BspPI GGATC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 412
BsrBI CCGCTC 3 cut(s) 260, 337, 384
BsrI ACTGG 1 cut(s) 325
BssECI CCNNGG 3 cut(s) 166, 167, 363
BssMI GATC 1 cut(s) 367
BssNI GRCGYC 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 4 cut(s) 292, 399, 412, 433
Bst6I CTCTTC 2 cut(s) 312, 397
BstACI GRCGYC 1 cut(s) 212
BstC8I GCNNGC 2 cut(s) 25, 335
BstEII GGTNACC 1 cut(s) 433
BstKTI GATC 1 cut(s) 370
BstMBI GATC 1 cut(s) 367
BstMWI GCNNNNNNNGC 4 cut(s) 39, 189, 232, 455
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 532
BstPI GGTNACC 1 cut(s) 433
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 230
BstSLI GKGCMC 1 cut(s) 528
BstV1I GCAGC 4 cut(s) 218, 222, 244, 515
BstXI CCANNNNNNTGG 1 cut(s) 445
BsuRI GGCC 3 cut(s) 333, 471, 537
BtgZI GCGATG 1 cut(s) 526
BtrI CACGTC 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 395
Cac8I GCNNGC 2 cut(s) 25, 335
Cfr13I GGNCC 2 cut(s) 332, 470
CseI GACGC 2 cut(s) 22, 201
CviAII CATG 7 cut(s) 37, 101, 352, 371, 376, 508, 529
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
DrdI GACNNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 290
Eam1104I CTCTTC 2 cut(s) 312, 397
EarI CTCTTC 2 cut(s) 312, 397
EciI GGCGGA 1 cut(s) 332
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 363
Eco147I AGGCCT 1 cut(s) 537
Eco24I GRGCYC 1 cut(s) 324
Eco53kI GAGCTC 1 cut(s) 322
Eco91I GGTNACC 1 cut(s) 433
EcoICRI GAGCTC 1 cut(s) 322
EcoO65I GGTNACC 1 cut(s) 433
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 7 cut(s) 40, 104, 355, 374, 379, 511, 532
FatI CATG 7 cut(s) 36, 100, 351, 370, 375, 507, 528
FauI CCCGC 1 cut(s) 342
FauNDI CATATG 1 cut(s) 49
Fnu4HI GCNGC 6 cut(s) 207, 210, 233, 236, 258, 504
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 6 cut(s) 207, 210, 233, 236, 258, 504
FspBI CTAG 3 cut(s) 185, 533, 553
GluI GCNGC 6 cut(s) 207, 210, 233, 236, 258, 504
GsuI CTGGAG 1 cut(s) 308
HaeIII GGCC 3 cut(s) 333, 471, 537
HapII CCGG 1 cut(s) 330
HgaI GACGC 2 cut(s) 22, 201
Hin1I GRCGYC 1 cut(s) 212
Hin1II CATG 7 cut(s) 40, 104, 355, 374, 379, 511, 532
HindIII AAGCTT 1 cut(s) 341
HpaII CCGG 1 cut(s) 330
HphI GGTGA 2 cut(s) 280, 445
Hpy166II GTNNAC 1 cut(s) 490
Hpy8I GTNNAC 1 cut(s) 490
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 2 cut(s) 269, 415
HpyCH4III ACNGT 4 cut(s) 292, 399, 412, 433
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 6 cut(s) 232, 238, 303, 355, 379, 503
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 189, 232, 455
HpySE526I ACGT 1 cut(s) 246
Hsp92I GRCGYC 1 cut(s) 212
Hsp92II CATG 7 cut(s) 40, 104, 355, 374, 379, 511, 532
Kzo9I GATC 1 cut(s) 367
LmnI GCTCC 3 cut(s) 319, 327, 455
LpnPI CCDG 6 cut(s) 9, 153, 180, 338, 343, 429
Lsp1109I GCAGC 4 cut(s) 218, 222, 244, 515
LweI GCATC 1 cut(s) 115
MaeI CTAG 3 cut(s) 185, 533, 553
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 1 cut(s) 433
MalI GATC 1 cut(s) 369
MbiI CCGCTC 3 cut(s) 260, 337, 384
MboI GATC 1 cut(s) 367
MboII GAAGA 2 cut(s) 299, 384
MfeI CAATTG 1 cut(s) 28
MhlI GDGCHC 2 cut(s) 324, 528
MluCI AATT 2 cut(s) 28, 58
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 5 cut(s) 108, 113, 187, 359, 548
MroXI GAANNNNTTC 1 cut(s) 391
MseI TTAA 2 cut(s) 152, 423
MspA1I CMGCKG 2 cut(s) 209, 235
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 1 cut(s) 168
MunI CAATTG 1 cut(s) 28
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 4 cut(s) 39, 189, 232, 455
NdeI CATATG 1 cut(s) 49
NdeII GATC 1 cut(s) 367
NlaIII CATG 7 cut(s) 40, 104, 355, 374, 379, 511, 532
NlaIV GGNNCC 1 cut(s) 414
NmuCI GTSAC 1 cut(s) 433
NspI RCATGY 1 cut(s) 532
PasI CCCWGGG 1 cut(s) 167
PceI AGGCCT 1 cut(s) 537
PdmI GAANNNNTTC 1 cut(s) 391
PkrI GCNGC 6 cut(s) 208, 211, 234, 237, 259, 505
PshBI ATTAAT 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 166
PspEI GGTNACC 1 cut(s) 433
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 2 cut(s) 332, 470
PstI CTGCAG 1 cut(s) 234
PvuII CAGCTG 1 cut(s) 235
SacI GAGCTC 1 cut(s) 324
SaqAI TTAA 2 cut(s) 152, 423
SatI GCNGC 6 cut(s) 207, 210, 233, 236, 258, 504
Sau3AI GATC 1 cut(s) 367
Sau96I GGNCC 2 cut(s) 332, 470
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 2 cut(s) 324, 528
SetI ASST 8 cut(s) 224, 237, 249, 324, 345, 460, 501, 550
SfaNI GCATC 1 cut(s) 115
SfcI CTRYAG 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 2 cut(s) 28, 58
SseBI AGGCCT 1 cut(s) 537
SsiI CCGC 5 cut(s) 209, 258, 317, 335, 384
SspMI CTAG 3 cut(s) 185, 533, 553
SstI GAGCTC 1 cut(s) 324
StuI AGGCCT 1 cut(s) 537
StyD4I CCNGG 1 cut(s) 166
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 4 cut(s) 292, 399, 412, 433
TaiI ACGT 1 cut(s) 249
TaqI TCGA 2 cut(s) 310, 465
TasI AATT 2 cut(s) 28, 58
TauI GCSGC 2 cut(s) 212, 260
Tru1I TTAA 2 cut(s) 152, 423
Tru9I TTAA 2 cut(s) 152, 423
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 4 cut(s) 206, 232, 235, 503
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 2 cut(s) 36, 498
TspRI CASTG 1 cut(s) 402
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 1 cut(s) 58
XceI RCATGY 1 cut(s) 532
XmnI GAANNNNTTC 1 cut(s) 391
XspI CTAG 3 cut(s) 185, 533, 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.