pycom15g10870
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003990
Physical Location & Seq
Reverse (-)
7304908 .. 7306679
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
ATGATGTTATTTGTGGAAAATGTTGTGGGATTTGAGACATCTGATACGCATACAAAAATGATAGAGATATTGGGAAGAACAAATTTTGTTATGCAGGAGTTCATATTGAGTCCGTTACAGTTTGGTGTACCCTATTCCAGGCCACGCTATTTTTGCCTGGCAAAGAGGAAACCTTCAACCTTTCACAATCAAGTTTTCGATAATCAGCTTCTTCACTCGCTGGGGCCATTATTAGGGGATACAAATGCCGATGAGACAGTGCTCTGTGAACTTGATGAACCACAAAAGAGCCAGGATAAGTTACTTGAATCTTGTGAGCCTATAGAAAGCTTTCTTGAATTCAAGAATTGCAGTGACCAATCGGAGTCCAATTTTGTGGACACCACTACTAGTTCTACAGATACTGATGGAATATTGGAGAAAGATGAAGGGAATGGATATTGTACCGGCATCGTAGATCAGTACATTGTTCCATTAAGCTTGATAGAGAGGTGGGGAAGTGCCATGGATATAGTCTATCCTGATTCGAAACGATGTTGTTGTTTTACAAAAAGTTACTATCGATATGTGAAGGGTACAGGCTCCCTTTTGGCAACTCTGCAGCCAAAGAATAAGGATAAAACATCTTCATTGAAGGAGCATCACCTTCGATACTTTTCCCCAAGGGAGGTTGCTAATTTGCATTCTCTCCCGGAAGATTTTCAGTTTCCACAGCAGATAAGCCTCAGGCAGCGTTATGCATTACTGGGGAACAGTTTGAGTATAGCAGTGGTTGCTCCTTTACTCAAATACTTATTTGCCGGATCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

271

Amino Acids

30.61

Weight (kDa)

5.3

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 2 - 263 6.5e-16 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 811
AcsI RAATTY 2 cut(s) 82, 338
AfaI GTAC 4 cut(s) 129, 445, 464, 577
AfiI CCNNNNNNNGG 3 cut(s) 138, 233, 667
AgsI TTSAA 5 cut(s) 177, 308, 338, 343, 634
AhlI ACTAGT 1 cut(s) 389
AjnI CCWGG 3 cut(s) 137, 156, 291
AluBI AGCT 3 cut(s) 208, 330, 480
AluI AGCT 3 cut(s) 208, 330, 480
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 2 cut(s) 29, 248
AlwI GGATC 1 cut(s) 811
AlwNI CAGNNNCTG 1 cut(s) 404
AoxI GGCC 2 cut(s) 140, 224
ApeKI GCWGC 2 cut(s) 601, 730
ApoI RAATTY 2 cut(s) 82, 338
Asp700I GAANNNNTTC 2 cut(s) 330, 699
AspS9I GGNCC 1 cut(s) 224
AsuC2I CCSGG 1 cut(s) 692
AsuHPI GGTGA 1 cut(s) 635
AsuII TTCGAA 1 cut(s) 527
AxyI CCTNAGG 1 cut(s) 725
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 613, 742
BccI CCATC 1 cut(s) 401
BcgI CGANNNNNNTGC 2 cut(s) 629, 663
BciT130I CCWGG 3 cut(s) 139, 158, 293
BciVI GTATCC 1 cut(s) 232
BcnI CCSGG 1 cut(s) 692
BcoDI GTCTC 2 cut(s) 29, 248
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 3 cut(s) 321, 396, 599
BfuI GTATCC 1 cut(s) 232
BisI GCNGC 2 cut(s) 602, 731
BlsI GCNGC 2 cut(s) 603, 732
Bme1390I CCNGG 4 cut(s) 139, 158, 293, 692
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 2 cut(s) 225, 583
BmrFI CCNGG 4 cut(s) 139, 158, 293, 692
BmrI ACTGGG 1 cut(s) 755
BmsI GCATC 2 cut(s) 459, 649
BmuI ACTGGG 1 cut(s) 755
Bpu14I TTCGAA 1 cut(s) 527
BpuMI CCSGG 1 cut(s) 692
Bsa29I ATCGAT 1 cut(s) 562
BsaJI CCNNGG 2 cut(s) 504, 662
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 233, 667
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 1 cut(s) 750
Bse21I CCTNAGG 1 cut(s) 725
BseBI CCWGG 3 cut(s) 139, 158, 293
BseCI ATCGAT 1 cut(s) 562
BseDI CCNNGG 2 cut(s) 504, 662
BseLI CCNNNNNNNGG 3 cut(s) 138, 233, 667
BseMII CTCAG 1 cut(s) 739
BseNI ACTGG 1 cut(s) 750
BseXI GCAGC 2 cut(s) 613, 742
BseYI CCCAGC 1 cut(s) 220
BshFI GGCC 2 cut(s) 142, 226
BshVI ATCGAT 1 cut(s) 562
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 3 cut(s) 447, 692, 801
BslI CCNNNNNNNGG 3 cut(s) 138, 233, 667
BsmAI GTCTC 2 cut(s) 29, 248
BsmI GAATGC 1 cut(s) 682
BsnI GGCC 2 cut(s) 142, 226
Bsp119I TTCGAA 1 cut(s) 527
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 2 cut(s) 457, 803
Bsp19I CCATGG 1 cut(s) 504
BspANI GGCC 2 cut(s) 142, 226
BspCNI CTCAG 1 cut(s) 738
BspDI ATCGAT 1 cut(s) 562
BspHI TCATGA 1 cut(s) 809
BspLI GGNNCC 2 cut(s) 225, 583
BspMAI CTGCAG 1 cut(s) 603
BspPI GGATC 1 cut(s) 811
BspT104I TTCGAA 1 cut(s) 527
BsrFI RCCGGY 1 cut(s) 446
BsrI ACTGG 1 cut(s) 750
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 2 cut(s) 504, 662
BssMI GATC 2 cut(s) 457, 803
BssT1I CCWWGG 2 cut(s) 504, 662
Bst2UI CCWGG 3 cut(s) 139, 158, 293
Bst4CI ACNGT 3 cut(s) 120, 259, 755
BstAPI GCANNNNNTGC 1 cut(s) 773
BstBI TTCGAA 1 cut(s) 527
BstDEI CTNAG 1 cut(s) 725
BstDSI CCRYGG 1 cut(s) 504
BstENI CCTNNNNNAGG 1 cut(s) 136
BstKTI GATC 2 cut(s) 460, 806
BstMAI GTCTC 2 cut(s) 29, 248
BstMBI GATC 2 cut(s) 457, 803
BstMWI GCNNNNNNNGC 2 cut(s) 153, 773
BstNI CCWGG 3 cut(s) 139, 158, 293
BstSCI CCNGG 4 cut(s) 137, 156, 291, 690
BstSFI CTRYAG 3 cut(s) 321, 396, 599
BstV1I GCAGC 2 cut(s) 613, 742
BstXI CCANNNNNNTGG 1 cut(s) 376
Bsu15I ATCGAT 1 cut(s) 562
Bsu36I CCTNAGG 1 cut(s) 725
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 2 cut(s) 142, 226
BsuTUI ATCGAT 1 cut(s) 562
BtgI CCRYGG 1 cut(s) 504
BtsI GCAGTG 2 cut(s) 358, 774
BtsIMutI CAGTG 3 cut(s) 264, 358, 774
CaiI CAGNNNCTG 1 cut(s) 404
CciI TCATGA 1 cut(s) 809
Cfr10I RCCGGY 1 cut(s) 446
Cfr13I GGNCC 1 cut(s) 224
ClaI ATCGAT 1 cut(s) 562
Csp6I GTAC 4 cut(s) 128, 444, 463, 576
CviAII CATG 2 cut(s) 505, 810
CviQI GTAC 4 cut(s) 128, 444, 463, 576
DdeI CTNAG 1 cut(s) 725
DpnI GATC 2 cut(s) 459, 805
DpnII GATC 2 cut(s) 457, 803
Eco130I CCWWGG 2 cut(s) 504, 662
Eco81I CCTNAGG 1 cut(s) 725
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 338
EcoRII CCWGG 3 cut(s) 137, 156, 291
EcoT14I CCWWGG 2 cut(s) 504, 662
EcoT22I ATGCAT 1 cut(s) 742
ErhI CCWWGG 2 cut(s) 504, 662
FaeI CATG 2 cut(s) 508, 813
FatI CATG 2 cut(s) 504, 809
Fnu4HI GCNGC 2 cut(s) 602, 731
Fsp4HI GCNGC 2 cut(s) 602, 731
FspBI CTAG 1 cut(s) 390
GluI GCNGC 2 cut(s) 602, 731
GsaI CCCAGC 1 cut(s) 224
HaeIII GGCC 2 cut(s) 142, 226
HapII CCGG 3 cut(s) 447, 692, 801
Hin1II CATG 2 cut(s) 508, 813
HindIII AAGCTT 2 cut(s) 328, 478
HinfI GANTC 4 cut(s) 109, 308, 365, 524
HpaII CCGG 3 cut(s) 447, 692, 801
HphI GGTGA 1 cut(s) 635
Hpy166II GTNNAC 3 cut(s) 128, 269, 379
Hpy188I TCNGA 2 cut(s) 43, 364
Hpy188III TCNNGA 4 cut(s) 335, 343, 521, 810
Hpy8I GTNNAC 3 cut(s) 128, 269, 379
HpyAV CCTTC 5 cut(s) 183, 422, 565, 628, 656
HpyCH4III ACNGT 3 cut(s) 120, 259, 755
HpyCH4V TGCA 5 cut(s) 94, 351, 601, 682, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 773
HpyF3I CTNAG 1 cut(s) 725
Hsp92II CATG 2 cut(s) 508, 813
Kzo9I GATC 2 cut(s) 457, 803
LmnI GCTCC 3 cut(s) 587, 637, 781
Lsp1109I GCAGC 2 cut(s) 613, 742
LweI GCATC 2 cut(s) 459, 649
MaeI CTAG 1 cut(s) 390
MaeIII GTNAC 4 cut(s) 114, 300, 353, 554
MalI GATC 2 cut(s) 459, 805
MboI GATC 2 cut(s) 457, 803
MboII GAAGA 4 cut(s) 87, 203, 618, 707
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 5 cut(s) 82, 338, 346, 370, 676
MlyI GAGTC 2 cut(s) 118, 374
MnlI CCTC 4 cut(s) 159, 483, 661, 734
Mph1103I ATGCAT 1 cut(s) 742
MroXI GAANNNNTTC 2 cut(s) 330, 699
MseI TTAA 1 cut(s) 476
MspI CCGG 3 cut(s) 447, 692, 801
MspR9I CCNGG 4 cut(s) 139, 158, 293, 692
Mva1269I GAATGC 1 cut(s) 682
MvaI CCWGG 3 cut(s) 139, 158, 293
MwoI GCNNNNNNNGC 2 cut(s) 153, 773
NciI CCSGG 1 cut(s) 692
NcoI CCATGG 1 cut(s) 504
NdeII GATC 2 cut(s) 457, 803
NlaIII CATG 2 cut(s) 508, 813
NlaIV GGNNCC 2 cut(s) 225, 583
NmuCI GTSAC 1 cut(s) 353
NsiI ATGCAT 1 cut(s) 742
NspV TTCGAA 1 cut(s) 527
PagI TCATGA 1 cut(s) 809
PctI GAATGC 1 cut(s) 682
PdmI GAANNNNTTC 2 cut(s) 330, 699
PfeI GAWTC 2 cut(s) 308, 524
PfoI TCCNGGA 1 cut(s) 690
PkrI GCNGC 2 cut(s) 603, 732
PleI GAGTC 2 cut(s) 117, 373
PpsI GAGTC 2 cut(s) 117, 373
Psp6I CCWGG 3 cut(s) 137, 156, 291
PspFI CCCAGC 1 cut(s) 220
PspGI CCWGG 3 cut(s) 137, 156, 291
PspN4I GGNNCC 2 cut(s) 225, 583
PspPI GGNCC 1 cut(s) 224
PstI CTGCAG 1 cut(s) 603
PstNI CAGNNNCTG 1 cut(s) 404
RsaI GTAC 4 cut(s) 129, 445, 464, 577
RsaNI GTAC 4 cut(s) 128, 444, 463, 576
SaqAI TTAA 1 cut(s) 476
SatI GCNGC 2 cut(s) 602, 731
Sau3AI GATC 2 cut(s) 457, 803
Sau96I GGNCC 1 cut(s) 224
SchI GAGTC 2 cut(s) 118, 374
ScrFI CCNGG 4 cut(s) 139, 158, 293, 692
SduI GDGCHC 1 cut(s) 264
SetI ASST 8 cut(s) 175, 182, 210, 332, 482, 494, 648, 672
SfaNI GCATC 2 cut(s) 459, 649
SfcI CTRYAG 3 cut(s) 321, 396, 599
SfuI TTCGAA 1 cut(s) 527
SpeI ACTAGT 1 cut(s) 389
Sse9I AATT 5 cut(s) 82, 338, 346, 370, 676
SspI AATATT 1 cut(s) 414
SspMI CTAG 1 cut(s) 390
StyD4I CCNGG 4 cut(s) 137, 156, 291, 690
StyI CCWWGG 2 cut(s) 504, 662
TaaI ACNGT 3 cut(s) 120, 259, 755
TaqI TCGA 4 cut(s) 198, 527, 562, 649
TasI AATT 5 cut(s) 82, 338, 346, 370, 676
TatI WGTACW 1 cut(s) 462
TfiI GAWTC 2 cut(s) 308, 524
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TscAI CASTG 3 cut(s) 264, 358, 774
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 2 cut(s) 601, 730
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 4 cut(s) 91, 291, 441, 618
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 3 cut(s) 264, 358, 774
XagI CCTNNNNNAGG 1 cut(s) 136
XapI RAATTY 2 cut(s) 82, 338
XmnI GAANNNNTTC 2 cut(s) 330, 699
XspI CTAG 1 cut(s) 390
Zsp2I ATGCAT 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.