pycom15g11290

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004715
Physical Location & Seq
Reverse (-)
7618681 .. 7621430
2750 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1290 bp
ATGAATGGGACCTTCAGCGAGAATACTATGACGGATACTGCAGCATCAAACGGGAAAATTGAAAATGTTGATAAGCAGGATGACGGTGTTAATAATAGCTCATCTATTTGGAAAGCCAAAGAGGCCAATGTCAACCCTGAAAGCAATGGTTTGACTGTTGCTTTGACTATTGCTAAGGAAGACGAAGTGAAAGGTTCTACTCACCCAAAACCCGCCAAAGTGCATAAAGGTCCAGGGAAGAGCAAGAATGAGAAGGCCCCTGGTGCCAAGAGCGTCTCCCCAATTTCGATGAAGAAAAGCAAAGATGGAAATGGCGCAGAGGCTAAGGCTTCTGTTTCGAATGGTTCAGCTGCTCCAAATTCACGCCCCAAACAGCCTAACAAGAGTGAATCATTTAATGGGCGACAGGTTCAGTCATCCAATCAGCAACCTGAAAAACCTGATGCAGAATTGTCTGAAAGCAGCGTGGAGAAGGCAAAGCTGAAGCCTTTGAAAAAGGGATCCCAGAATCAGGCTGAAGGAGAATCTCAATCCTCTTTAAGTCCTACAGAAGGAGATAAACATCCTAGGGTTGGTACACTTCCAAACTATGGTTTCAGTTTTAGGTGCGATGAACGAGCTGAGAAAAGGAGAGAGTTCTATACGAAAATGGAGGAAAAGATACATGCAAAGGAGATGGAAAAGAATAATTTGCAAGCCAAGTCTAAGGAAACTCTAGAAGCTGAGATTAGGATGCTCAGGAAGAAACTGACTTTCAAAGCAACTCCAATGCCAAGCTTCTATCAGGAGCCTCCACCTCCTAAGGTGGAGCTGAAGAAGATACCAACAACAAGAGCCAAGTCTCCCAAGCTCGGACGTAGGAAGAGCTTACCTCCTGCAGTTTCTGAAGCAAATGGTAATACAAATGATCAATCTAGCAGGCTTAGTCTGGATCAGAAAGTCCCTCAGAACACTTCTAAAGGGCCCTCTCCTGTACATCCAAAGAAGCCACAACGGAAATCTCTTCCTAGACTCCCGTCTGAGAAGACTACTTTACCTAATGCAGTGAATGAACGAAAAACGACTTTTAAGGCAGTAAACAAGGAAAAGACCAACTTAATTGCTGCAATGAATGAGAATGCCACCGTGCCCACTGCAGAAAGTGAAGCTGGATCTCACACCCAAGATCAGGAGGCAGCAGTTCCCAAAGCCAAGGTAAGCGAAGAGCAGTCCCATACAGATGACGAGACAGCCGATGAAGAGCAACATCAGCCCACCTACATGCAAGAACCTATAGCCTCGAAGCATTGA

Protein Analysis

430

Amino Acids

46.89

Weight (kDa)

9.42

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 199 - 274 6.1e-27 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 263
AccB7I CCANNNNNTGG 1 cut(s) 590
AciI CCGC 1 cut(s) 213
AclWI GGATC 4 cut(s) 495, 508, 939, 1159
AcsI RAATTY 1 cut(s) 358
AcuI CTGAAG 4 cut(s) 503, 537, 833, 906
AfaI GTAC 2 cut(s) 577, 975
AfiI CCNNNNNNNGG 6 cut(s) 511, 551, 572, 590, 851, 1168
AgsI TTSAA 3 cut(s) 62, 493, 757
AjnI CCWGG 2 cut(s) 232, 259
Alw26I GTCTC 3 cut(s) 280, 846, 1220
AlwI GGATC 4 cut(s) 495, 508, 939, 1159
AlwNI CAGNNNCTG 1 cut(s) 884
AoxI GGCC 3 cut(s) 123, 255, 962
ApaI GGGCCC 1 cut(s) 966
ApeKI GCWGC 5 cut(s) 41, 350, 462, 1103, 1175
ApoI RAATTY 1 cut(s) 358
ArsI GACNNNNNNTTYG 2 cut(s) 145, 177
AspA2I CCTAGG 1 cut(s) 566
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 5 cut(s) 9, 230, 256, 962, 963
AsuHPI GGTGA 1 cut(s) 194
AsuII TTCGAA 1 cut(s) 338
AvaII GGWCC 2 cut(s) 9, 230
AvrII CCTAGG 1 cut(s) 566
AxyI CCTNAGG 1 cut(s) 801
BaeGI GKGCMC 2 cut(s) 966, 1131
BamHI GGATCC 1 cut(s) 500
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 966
BbsI GAAGAC 2 cut(s) 186, 1031
BbvI GCAGC 5 cut(s) 53, 337, 474, 1090, 1187
BccI CCATC 2 cut(s) 299, 670
BciT130I CCWGG 2 cut(s) 234, 261
BciVI GTATCC 1 cut(s) 28
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 3 cut(s) 280, 846, 1220
BfaI CTAG 4 cut(s) 567, 716, 915, 1008
BfmI CTRYAG 5 cut(s) 39, 546, 876, 1134, 1272
BfuI GTATCC 1 cut(s) 28
BglI GCCNNNNNGGC 1 cut(s) 122
BisI GCNGC 5 cut(s) 42, 351, 463, 1104, 1176
BlnI CCTAGG 1 cut(s) 566
BlsI GCNGC 5 cut(s) 43, 352, 464, 1105, 1177
Bme1390I CCNGG 2 cut(s) 234, 261
Bme18I GGWCC 2 cut(s) 9, 230
BmgT120I GGNCC 5 cut(s) 9, 230, 256, 962, 963
BmiI GGNNCC 6 cut(s) 10, 258, 265, 502, 789, 964
BmrFI CCNGG 2 cut(s) 234, 261
BmsI GCATC 3 cut(s) 53, 433, 723
BoxI GACNNNNGTC 1 cut(s) 1015
BpiI GAAGAC 2 cut(s) 186, 1031
BplI GAGNNNNNCTC 2 cut(s) 856, 888
Bpu10I CCTNAGC 3 cut(s) 174, 324, 737
Bpu14I TTCGAA 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 561
BsaJI CCNNGG 4 cut(s) 233, 259, 566, 1191
Bsc4I CCNNNNNNNGG 6 cut(s) 511, 551, 572, 590, 851, 1168
Bse21I CCTNAGG 1 cut(s) 801
Bse3DI GCAATG 2 cut(s) 151, 1113
Bse8I GATNNNNATC 1 cut(s) 561
BseBI CCWGG 2 cut(s) 234, 261
BseDI CCNNGG 4 cut(s) 233, 259, 566, 1191
BseGI GGATG 5 cut(s) 85, 416, 562, 738, 976
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 6 cut(s) 511, 551, 572, 590, 851, 1168
BseMI GCAATG 2 cut(s) 151, 1113
BseMII CTCAG 5 cut(s) 612, 714, 751, 959, 1011
BseSI GKGCMC 2 cut(s) 966, 1131
BseXI GCAGC 5 cut(s) 53, 337, 474, 1090, 1187
BshFI GGCC 3 cut(s) 125, 257, 964
BshNI GGYRCC 1 cut(s) 263
BslFI GGGAC 3 cut(s) 22, 926, 1195
BslI CCNNNNNNNGG 6 cut(s) 511, 551, 572, 590, 851, 1168
BsmAI GTCTC 3 cut(s) 280, 846, 1220
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 3 cut(s) 22, 926, 1195
BsmI GAATGC 1 cut(s) 1123
BsnI GGCC 3 cut(s) 125, 257, 964
Bsp119I TTCGAA 1 cut(s) 338
Bsp120I GGGCCC 1 cut(s) 962
Bsp1286I GDGCHC 2 cut(s) 966, 1131
Bsp1407I TGTACA 1 cut(s) 973
Bsp143I GATC 5 cut(s) 500, 907, 931, 1151, 1165
BspACI CCGC 1 cut(s) 213
BspANI GGCC 3 cut(s) 125, 257, 964
BspCNI CTCAG 5 cut(s) 613, 715, 750, 958, 1012
BspLI GGNNCC 6 cut(s) 10, 258, 265, 502, 789, 964
BspMAI CTGCAG 3 cut(s) 43, 880, 1138
BspPI GGATC 4 cut(s) 495, 508, 939, 1159
BspQI GCTCTTC 4 cut(s) 233, 857, 1197, 1233
BspT104I TTCGAA 1 cut(s) 338
BspT107I GGYRCC 1 cut(s) 263
BsrDI GCAATG 2 cut(s) 151, 1113
BsrGI TGTACA 1 cut(s) 973
BssECI CCNNGG 4 cut(s) 233, 259, 566, 1191
BssMI GATC 5 cut(s) 500, 907, 931, 1151, 1165
BssT1I CCWWGG 2 cut(s) 566, 1191
Bst2UI CCWGG 2 cut(s) 234, 261
Bst4CI ACNGT 3 cut(s) 86, 157, 1126
Bst6I CTCTTC 5 cut(s) 233, 857, 1008, 1197, 1233
BstAUI TGTACA 1 cut(s) 973
BstBI TTCGAA 1 cut(s) 338
BstC8I GCNNGC 2 cut(s) 696, 920
BstENI CCTNNNNNAGG 1 cut(s) 549
BstF5I GGATG 5 cut(s) 85, 416, 562, 738, 976
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 5 cut(s) 503, 910, 934, 1154, 1168
BstMAI GTCTC 3 cut(s) 280, 846, 1220
BstMBI GATC 5 cut(s) 500, 907, 931, 1151, 1165
BstMWI GCNNNNNNNGC 3 cut(s) 122, 263, 1249
BstNI CCWGG 2 cut(s) 234, 261
BstNSI RCATGY 2 cut(s) 668, 1264
BstPAI GACNNNNGTC 1 cut(s) 1015
BstSCI CCNGG 2 cut(s) 232, 259
BstSFI CTRYAG 5 cut(s) 39, 546, 876, 1134, 1272
BstSLI GKGCMC 2 cut(s) 966, 1131
BstV1I GCAGC 5 cut(s) 53, 337, 474, 1090, 1187
BstV2I GAAGAC 2 cut(s) 186, 1031
BstX2I RGATCY 2 cut(s) 500, 1151
BstYI RGATCY 2 cut(s) 500, 1151
Bsu36I CCTNAGG 1 cut(s) 801
BsuI GTATCC 1 cut(s) 28
BsuRI GGCC 3 cut(s) 125, 257, 964
BtgZI GCGATG 1 cut(s) 624
BtsCI GGATG 5 cut(s) 85, 416, 562, 738, 976
BtsI GCAGTG 2 cut(s) 1050, 1131
BtsIMutI CAGTG 2 cut(s) 1050, 1131
Cac8I GCNNGC 2 cut(s) 696, 920
CaiI CAGNNNCTG 1 cut(s) 884
CfoI GCGC 1 cut(s) 317
Cfr13I GGNCC 5 cut(s) 9, 230, 256, 962, 963
CseI GACGC 1 cut(s) 262
Csp6I GTAC 2 cut(s) 576, 974
CviAII CATG 2 cut(s) 665, 1261
CviQI GTAC 2 cut(s) 576, 974
DpnI GATC 5 cut(s) 502, 909, 933, 1153, 1167
DpnII GATC 5 cut(s) 500, 907, 931, 1151, 1165
Eam1104I CTCTTC 5 cut(s) 233, 857, 1008, 1197, 1233
EarI CTCTTC 5 cut(s) 233, 857, 1008, 1197, 1233
Eco130I CCWWGG 2 cut(s) 566, 1191
Eco24I GRGCYC 1 cut(s) 966
Eco47I GGWCC 2 cut(s) 9, 230
Eco57I CTGAAG 4 cut(s) 503, 537, 833, 906
Eco81I CCTNAGG 1 cut(s) 801
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoO109I RGGNCCY 4 cut(s) 9, 256, 962, 963
EcoRII CCWGG 2 cut(s) 232, 259
EcoT14I CCWWGG 2 cut(s) 566, 1191
EcoT38I GRGCYC 1 cut(s) 966
ErhI CCWWGG 2 cut(s) 566, 1191
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 2 cut(s) 668, 1264
FaiI YATR 8 cut(s) 29, 225, 591, 642, 666, 1215, 1262, 1274
FalI AAGNNNNNCTT 2 cut(s) 1079, 1111
FaqI GGGAC 3 cut(s) 22, 926, 1195
FatI CATG 2 cut(s) 664, 1260
FauI CCCGC 1 cut(s) 220
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 5 cut(s) 42, 351, 463, 1104, 1176
FokI GGATG 5 cut(s) 92, 403, 549, 745, 963
FriOI GRGCYC 1 cut(s) 966
Fsp4HI GCNGC 5 cut(s) 42, 351, 463, 1104, 1176
FspBI CTAG 4 cut(s) 567, 716, 915, 1008
GlaI GCGC 1 cut(s) 316
GluI GCNGC 5 cut(s) 42, 351, 463, 1104, 1176
HaeIII GGCC 3 cut(s) 125, 257, 964
HgaI GACGC 1 cut(s) 262
HhaI GCGC 1 cut(s) 317
Hin1II CATG 2 cut(s) 668, 1264
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 775
HinfI GANTC 4 cut(s) 389, 508, 524, 1011
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 3 cut(s) 133, 578, 1078
Hpy188I TCNGA 6 cut(s) 457, 854, 886, 936, 948, 1021
Hpy188III TCNNGA 5 cut(s) 716, 739, 785, 929, 1169
Hpy8I GTNNAC 3 cut(s) 133, 578, 1078
HpyAV CCTTC 5 cut(s) 22, 247, 466, 512, 545
HpyCH4III ACNGT 3 cut(s) 86, 157, 1126
HpyCH4IV ACGT 1 cut(s) 856
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 263, 1249
HpySE526I ACGT 1 cut(s) 856
Hsp92II CATG 2 cut(s) 668, 1264
HspAI GCGC 1 cut(s) 315
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 5 cut(s) 500, 907, 931, 1151, 1165
LguI GCTCTTC 4 cut(s) 233, 857, 1197, 1233
LmnI GCTCC 3 cut(s) 358, 787, 808
Lsp1109I GCAGC 5 cut(s) 53, 337, 474, 1090, 1187
LweI GCATC 3 cut(s) 53, 433, 723
MaeI CTAG 4 cut(s) 567, 716, 915, 1008
MaeII ACGT 1 cut(s) 856
MalI GATC 5 cut(s) 502, 909, 933, 1153, 1167
MboI GATC 5 cut(s) 500, 907, 931, 1151, 1165
MflI RGATCY 2 cut(s) 500, 1151
MhlI GDGCHC 2 cut(s) 966, 1131
MluCI AATT 6 cut(s) 57, 282, 358, 449, 688, 1098
MlyI GAGTC 1 cut(s) 1005
MseI TTAA 5 cut(s) 90, 396, 539, 1068, 1097
MslI CAYNNNNRTG 2 cut(s) 1218, 1259
MspA1I CMGCKG 1 cut(s) 350
MspR9I CCNGG 2 cut(s) 234, 261
Mva1269I GAATGC 1 cut(s) 1123
MvaI CCWGG 2 cut(s) 234, 261
MwoI GCNNNNNNNGC 3 cut(s) 122, 263, 1249
NdeII GATC 5 cut(s) 500, 907, 931, 1151, 1165
NlaIII CATG 2 cut(s) 668, 1264
NlaIV GGNNCC 6 cut(s) 10, 258, 265, 502, 789, 964
NspI RCATGY 2 cut(s) 668, 1264
NspV TTCGAA 1 cut(s) 338
PciSI GCTCTTC 4 cut(s) 233, 857, 1197, 1233
PcsI WCGNNNNNNNCGW 1 cut(s) 1230
PctI GAATGC 1 cut(s) 1123
PfeI GAWTC 3 cut(s) 389, 508, 524
PflMI CCANNNNNTGG 1 cut(s) 590
PkrI GCNGC 5 cut(s) 43, 352, 464, 1105, 1177
PleI GAGTC 1 cut(s) 1005
PpsI GAGTC 1 cut(s) 1005
PpuMI RGGWCCY 1 cut(s) 9
PshAI GACNNNNGTC 1 cut(s) 1015
Psp5II RGGWCCY 1 cut(s) 9
Psp6I CCWGG 2 cut(s) 232, 259
PspGI CCWGG 2 cut(s) 232, 259
PspN4I GGNNCC 6 cut(s) 10, 258, 265, 502, 789, 964
PspOMI GGGCCC 1 cut(s) 962
PspPI GGNCC 5 cut(s) 9, 230, 256, 962, 963
PspPPI RGGWCCY 1 cut(s) 9
PstI CTGCAG 3 cut(s) 43, 880, 1138
PstNI CAGNNNCTG 1 cut(s) 884
PsuI RGATCY 2 cut(s) 500, 1151
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 2 cut(s) 577, 975
RsaNI GTAC 2 cut(s) 576, 974
RseI CAYNNNNRTG 2 cut(s) 1218, 1259
SapI GCTCTTC 4 cut(s) 233, 857, 1197, 1233
SaqAI TTAA 5 cut(s) 90, 396, 539, 1068, 1097
SatI GCNGC 5 cut(s) 42, 351, 463, 1104, 1176
Sau3AI GATC 5 cut(s) 500, 907, 931, 1151, 1165
Sau96I GGNCC 5 cut(s) 9, 230, 256, 962, 963
SchI GAGTC 1 cut(s) 1005
ScrFI CCNGG 2 cut(s) 234, 261
SduI GDGCHC 2 cut(s) 966, 1131
SfaNI GCATC 3 cut(s) 53, 433, 723
SfcI CTRYAG 5 cut(s) 39, 546, 876, 1134, 1272
SfuI TTCGAA 1 cut(s) 338
SinI GGWCC 2 cut(s) 9, 230
SmiMI CAYNNNNRTG 2 cut(s) 1218, 1259
Sse9I AATT 6 cut(s) 57, 282, 358, 449, 688, 1098
SsiI CCGC 1 cut(s) 213
SspMI CTAG 4 cut(s) 567, 716, 915, 1008
StyD4I CCNGG 2 cut(s) 232, 259
StyI CCWWGG 2 cut(s) 566, 1191
TaaI ACNGT 3 cut(s) 86, 157, 1126
TaiI ACGT 1 cut(s) 859
TaqI TCGA 3 cut(s) 287, 338, 1280
TasI AATT 6 cut(s) 57, 282, 358, 449, 688, 1098
TatI WGTACW 1 cut(s) 973
TfiI GAWTC 3 cut(s) 389, 508, 524
Tru1I TTAA 5 cut(s) 90, 396, 539, 1068, 1097
Tru9I TTAA 5 cut(s) 90, 396, 539, 1068, 1097
TscAI CASTG 2 cut(s) 1050, 1138
TseI GCWGC 5 cut(s) 41, 350, 462, 1103, 1175
TspDTI ATGAA 6 cut(s) 17, 305, 627, 1065, 1124, 1251
TspGWI ACGGA 2 cut(s) 47, 1009
TspRI CASTG 2 cut(s) 1050, 1138
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 2 cut(s) 9, 230
XagI CCTNNNNNAGG 1 cut(s) 549
XapI RAATTY 1 cut(s) 358
XbaI TCTAGA 1 cut(s) 715
XceI RCATGY 2 cut(s) 668, 1264
XmaJI CCTAGG 1 cut(s) 566
XspI CTAG 4 cut(s) 567, 716, 915, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.