Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Forward (+)

9844869 .. 9848902

Gene Structure 4034 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2781 bp

ATGGATAACCAGGTTGTCCTTGCTGAGGACAAAAAGTATTATCCAACCGCTGAAGAGGTCTACGGTGAAGATGTTGAGACATTGGTTATGGATGAAGATGCACAGCCTCTGGAACAACCCATTATTAAGCCTGTTAGGAATGTCAAGTTTGAGGTTGGTGTCAAGGATTCTTCCACCTACGTGTCAAATCAGTTCCTTTTGGGACTCATGTCGAACCCGTCTCTGGTTCGGAACGTTGCACTTGTTGGGCATTTGCAGCATGGGAAGACGGTGTTCATGGATATGTTGGTAGAGCAGACTCACCATATGTCCACTTTTGATTCAAATAGCGAGAAGCACATGAGGTACACTGATACAAGGATTGATGAGCAAGAGAGGAGGATATCCATTAAAGCCGTTCCTATGTCCCTAGTACTCGAGGATAGCAGGTCTAAATCGTATTTGTGCAACATCATGGATACGCCTGGTCATGTCAATTTCTCTGATGAAATGACTGCAGCTCTCAGGCTTGCTGACGGTGCTGTCTTGATTGTGGACGCTGCAGAAGGAATGATGGTAAATACCGAGAGGGCTATACGCCATGCAATCCAAGAGCGACTACCAATTGTGGTTGTAATCAACAAGGTTGACAGATTGATAACAGAACTCAAATTGCCCCCAAGAGATGCATACTTCAAGATAAGGCATACTTTGGACGTCATTAATACCCACATAACTGCGGCCTCGTCAACTGCTGGCAATGTACAAACTATTGACCCTGTTGCAGGAAATGTTTGCTTTGCAAGTGCTACTGCAGGATGGTCATTCACGTTGCAATCGTTTGCAAAGCTATATCTTAAACTCCATGGGGCTGATAATACGGAGGTAGATAAGTTTGCTTCTCGTCTCTGGGGAGATATGTACTATCATCCGGATGACAGGAGTTTCAGGAGAAAACCCCCTGCCAGTGGAGGAGAAAGGTCATTTGTCCAGTTTGTGCTTGAACCCCTGTATAAAATATATAGCCAGGTCATTGGAGAACATAAAAAGAGTGTGGAGGGAACTCTTGCCGAATTTGGTGTCACGCTCAGCAATGCAGCTTACAAGTTGAATGTTAGACCTCTGCTGAGGTTGGCTTGTAGCTCAGTCTTTGGATATGCCTCAGGCTTTACTGATATGCTGGTTCAACATATTCCTTCCCCTAAAGGTGCCGCAACTAGGAAAGTAGACCATATATATACTGGACCAAAAGACTCTTCGATTTACAAGGCCATGAAAGATTGTGATCCTGATGGTCCTTTGATGGTCAATGTGACAAAATTGTATCCCAAGTCCGACTGTAGTGTGTTTGATGCATTTGGTAGGGTTTATAGTGGCAAGATTCAGACAGGGCAGACTGTACGGGTATTGGGAGAAGGTTATTCACCAGAGGATGAGGAGGACATGACAGTCAAAGAGGTGACAAAGTTATGGCTTTATCAAGCTCGAGACAGGATACCAATAGCCGAGGCTCCTCCTGGTTCTTGGGTTCTTATTGAAGGTGTGGATGCTTCTATTATGAAAACTGCCACCATCTGTAATGAATATTATGACGAGGATGTGTACATCTTCCGTCCTCTTCAGTTCAATACTCTGCCAGTTGTGAAAACAGCAACCGAGCCACTAAATCCAAGTGAGCTGCCAAAGATGGTGGAGGGACTGAGAAAGATAAGCAAGAGTTATCCTCTTGCAATCACTAAAGTTGAGGAGTCGGGGGAGCACACGATATTAGGGACAGGAGAACTCTACTTGGATTCAATAATGAAGGACCTAAGAGAGCTTTATTCGGAAGTAGAAGTCAAGGTTGCGGATCCCGTTGTTTCGTTCTGTGAAACAGTGGTAGAGTCTTCATCAATGAAATGTTTTGCTGAAACACCCAATAAGAAGAATAAAATAACTATGATTGCAGAGCCATTGGAGAGAGGACTTGCTGAGGACATTGAGAATGGTGTTGTAAGCATTGATTGGTCTCGGAAGGATATAGGCAATTTTTTCCAGACTAGATATGAGTGGGATGTTCTTGCTGCACGGTCCATATGGGCATTTGGCCCTGATAAGCAGGGACCTAACATTCTATTAGATGACACACTTTCCACTGAAGTTGACAAAAGCTTGCTAAATGCTGTGAAGGATTCGATTGTTCAAGGGTTTCAGTGGGGTGCTCGAGAAGGACCCCTTTGTGATGAACCAATCAGAAATGTAAAGTTCAAAATTGTGGATGCAAGGATTGCCCAAGAGCCATTACATCGTGGATCTGGCCAGATCATTCCCACATCTCGACGTGTGGCCTATTCATCTTTCCTGATGGCAACACCACGGCTTATGGAGCCAGTATATTATGTGGAGATACAAACACCCATAGACTGTATTTCTGCAATCTACACTGTTCTATCTCGCAGGCGTGGACATGTTACAGCTGATGTTCCTCAACCTGGGACCCCTGCTTATATCGTCAAGGCGTTCTTACCAGTGATTGAATCTTTTGGCTTTGAAACTGACTTGAGGTACCATACCCAAGGACAGGCTTTCTGCCTCTCAGTGTTCGATCACTGGGCTATAGTTCCTGGGGATCCACTTGATAAAAGCATCGTTCTGCGACCCCTTGAACCTGCCCCGATTCAGCATCTGGCCCGTGAGTTTATGGTCAAGACAAGACGTAGAAAGGGAATGAGCGAGGATGTGAGCATCAACAAATTCTTCGACGAGGCTATGATGGTGGAGCTGGCTCAGCAGGCAGCTGATCTTCATCTCCAAATGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

927

Amino Acids

103.85

Weight (kDa)

5.5

Isoelectric Point (pI)

39.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFTUD2	PF16004	2 - 56	2.3e-23	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
GTP_EFTU	PF00009	76 - 270	4.2e-42	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	445 - 519	8.5e-11	Elongation factor Tu domain 2
EFG_IV	PF03764	662 - 776	2.4e-19	Elongation factor G, domain IV
EFG_C	PF00679	779 - 867	3.4e-22	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	521, 1427
AatII	GACGTC	1 cut(s)	699
Acc36I	ACCTGC	2 cut(s)	417, 2635
Acc65I	GGTACC	1 cut(s)	2523
AccB1I	GGYRCC	2 cut(s)	1187, 2523
AccI	GTMKAC	2 cut(s)	60, 1206
AccIII	TCCGGA	1 cut(s)	910
AciI	CCGC	4 cut(s)	48, 719, 1191, 1826
AclI	AACGTT	1 cut(s)	234
AclWI	GGATC	6 cut(s)	1259, 1823, 1836, 2278, 2582, 2595
AcoI	YGGCCR	1 cut(s)	2275
AcsI	RAATTY	2 cut(s)	1052, 2711
AcuI	CTGAAG	3 cut(s)	72, 1583, 2136
AcyI	GRCGYC	1 cut(s)	696
AfaI	GTAC	7 cut(s)	347, 414, 744, 902, 1380, 1583, 2525
AfiI	CCNNNNNNNGG	7 cut(s)	25, 223, 764, 947, 1197, 2450, 2539
AflIII	ACRYGT	3 cut(s)	180, 2299, 2425
AjiI	CACGTC	1 cut(s)	2300
AjnI	CCWGG	6 cut(s)	9, 463, 1005, 1495, 2449, 2581
AjuI	GAANNNNNNNTTGG	2 cut(s)	1219, 1251
AleI	CACNNNNGTG	1 cut(s)	179
Alw21I	GWGCWC	2 cut(s)	1740, 2182
Alw26I	GTCTC	5 cut(s)	71, 225, 890, 1461, 1992
AlwI	GGATC	6 cut(s)	1259, 1823, 1836, 2278, 2582, 2595
AlwNI	CAGNNNCTG	2 cut(s)	109, 2644
Ama87I	CYCGRG	3 cut(s)	416, 1464, 2181
Aor13HI	TCCGGA	1 cut(s)	910
AoxI	GGCC	6 cut(s)	720, 1248, 2065, 2275, 2304, 2646
ApeKI	GCWGC	7 cut(s)	256, 497, 539, 1076, 1657, 2042, 2753
ApoI	RAATTY	2 cut(s)	1052, 2711
ArsI	GACNNNNNNTTYG	2 cut(s)	1044, 1076
AseI	ATTAAT	1 cut(s)	702
Asp718I	GGTACC	1 cut(s)	2523
AspS9I	GGNCC	9 cut(s)	1223, 1274, 1786, 2049, 2066, 2081, 2189, 2454, 2647
AsuHPI	GGTGA	4 cut(s)	77, 293, 1395, 1450
AvaI	CYCGRG	3 cut(s)	416, 1464, 2181
AvaII	GGWCC	7 cut(s)	1223, 1274, 1786, 2049, 2081, 2189, 2454
AxyI	CCTNAGG	1 cut(s)	1141
BalI	TGGCCA	1 cut(s)	2277
BamHI	GGATCC	2 cut(s)	1828, 2587
BanI	GGYRCC	2 cut(s)	1187, 2523
BbsI	GAAGAC	2 cut(s)	272, 1857
Bbv12I	GWGCWC	2 cut(s)	1740, 2182
BbvCI	CCTCAGC	3 cut(s)	24, 1106, 1950
BbvI	GCAGC	7 cut(s)	268, 509, 526, 1088, 1644, 2029, 2765
BccI	CCATC	8 cut(s)	547, 792, 1265, 1276, 1559, 1660, 2317, 2725
BceAI	ACGGC	2 cut(s)	380, 2351
BciT130I	CCWGG	6 cut(s)	11, 465, 1007, 1497, 2451, 2583
BciVI	GTATCC	3 cut(s)	451, 1314, 1467
BcoDI	GTCTC	5 cut(s)	71, 225, 890, 1461, 1992
BfaI	CTAG	3 cut(s)	410, 1197, 2019
BfmI	CTRYAG	5 cut(s)	495, 540, 792, 1318, 2574
BfuAI	ACCTGC	2 cut(s)	417, 2635
BfuI	GTATCC	3 cut(s)	451, 1314, 1467
BisI	GCNGC	9 cut(s)	257, 498, 540, 720, 1077, 1191, 1658, 2043, 2754
BlpI	GCTNAGC	2 cut(s)	1067, 2745
BlsI	GCNGC	9 cut(s)	258, 499, 541, 721, 1078, 1192, 1659, 2044, 2755
BmcAI	AGTACT	1 cut(s)	414
Bme1390I	CCNGG	6 cut(s)	11, 465, 1007, 1497, 2451, 2583
Bme18I	GGWCC	7 cut(s)	1223, 1274, 1786, 2049, 2081, 2189, 2454
BmeT110I	CYCGRG	3 cut(s)	416, 1464, 2181
BmgBI	CACGTC	1 cut(s)	2300
BmgT120I	GGNCC	9 cut(s)	1223, 1274, 1786, 2049, 2066, 2081, 2189, 2454, 2647
BmrFI	CCNGG	6 cut(s)	11, 465, 1007, 1497, 2451, 2583
BmrI	ACTGGG	1 cut(s)	2578
BmsI	GCATC	8 cut(s)	88, 655, 1321, 1516, 2227, 2613, 2650, 2712
BmuI	ACTGGG	1 cut(s)	2578
BoxI	GACNNNNGTC	1 cut(s)	208
BpiI	GAAGAC	2 cut(s)	272, 1857
BplI	GAGNNNNNCTC	2 cut(s)	1687, 1719
Bpu10I	CCTNAGC	3 cut(s)	24, 1106, 1950
Bpu1102I	GCTNAGC	2 cut(s)	1067, 2745
BpuEI	CTTGAG	1 cut(s)	2539
BsaAI	YACGTR	1 cut(s)	181
BsaBI	GATNNNNATC	2 cut(s)	1263, 2763
BsaHI	GRCGYC	1 cut(s)	696
BsaI	GGTCTC	1 cut(s)	1992
BsaJI	CCNNGG	6 cut(s)	844, 1485, 2333, 2450, 2533, 2582
BsaWI	WCCGGW	1 cut(s)	910
Bsc4I	CCNNNNNNNGG	7 cut(s)	25, 223, 764, 947, 1197, 2450, 2539
Bse1I	ACTGG	7 cut(s)	945, 970, 1225, 1616, 2348, 2486, 2573
Bse21I	CCTNAGG	1 cut(s)	1141
Bse3DI	GCAATG	2 cut(s)	745, 1078
Bse8I	GATNNNNATC	2 cut(s)	1263, 2763
BseAI	TCCGGA	1 cut(s)	910
BseBI	CCWGG	6 cut(s)	11, 465, 1007, 1497, 2451, 2583
BseDI	CCNNGG	6 cut(s)	844, 1485, 2333, 2450, 2533, 2582
BseJI	GATNNNNATC	2 cut(s)	1263, 2763
BseLI	CCNNNNNNNGG	7 cut(s)	25, 223, 764, 947, 1197, 2450, 2539
BseMI	GCAATG	2 cut(s)	745, 1078
BseNI	ACTGG	7 cut(s)	945, 970, 1225, 1616, 2348, 2486, 2573
BseRI	GAGGAG	5 cut(s)	391, 966, 1430, 1482, 1739
BseXI	GCAGC	7 cut(s)	268, 509, 526, 1088, 1644, 2029, 2765
BsgI	GTGCAG	1 cut(s)	2028
BshFI	GGCC	6 cut(s)	722, 1250, 2067, 2277, 2306, 2648
BshNI	GGYRCC	2 cut(s)	1187, 2523
BsiHKAI	GWGCWC	2 cut(s)	1740, 2182
BsiHKCI	CYCGRG	3 cut(s)	416, 1464, 2181
BsiSI	CCGG	1 cut(s)	911
BslFI	GGGAC	6 cut(s)	216, 391, 1689, 1765, 2094, 2467
BslI	CCNNNNNNNGG	7 cut(s)	25, 223, 764, 947, 1197, 2450, 2539
BsmAI	GTCTC	5 cut(s)	71, 225, 890, 1461, 1992
BsmBI	CGTCTC	2 cut(s)	225, 890
BsmFI	GGGAC	6 cut(s)	216, 391, 1689, 1765, 2094, 2467
BsnI	GGCC	6 cut(s)	722, 1250, 2067, 2277, 2306, 2648
Bso31I	GGTCTC	1 cut(s)	1992
BsoBI	CYCGRG	3 cut(s)	416, 1464, 2181
Bsp1286I	GDGCHC	2 cut(s)	1740, 2182
Bsp13I	TCCGGA	1 cut(s)	910
Bsp1407I	TGTACA	2 cut(s)	742, 1581
Bsp143I	GATC	7 cut(s)	1264, 1828, 2270, 2280, 2563, 2587, 2758
Bsp1720I	GCTNAGC	2 cut(s)	1067, 2745
Bsp19I	CCATGG	1 cut(s)	844
BspACI	CCGC	4 cut(s)	48, 719, 1191, 1826
BspANI	GGCC	6 cut(s)	722, 1250, 2067, 2277, 2306, 2648
BspEI	TCCGGA	1 cut(s)	910
BspMAI	CTGCAG	3 cut(s)	499, 544, 796
BspMI	ACCTGC	2 cut(s)	417, 2635
BspPI	GGATC	6 cut(s)	1259, 1823, 1836, 2278, 2582, 2595
BspT107I	GGYRCC	2 cut(s)	1187, 2523
BspTNI	GGTCTC	1 cut(s)	1992
BsrDI	GCAATG	2 cut(s)	745, 1078
BsrGI	TGTACA	2 cut(s)	742, 1581
BsrI	ACTGG	7 cut(s)	945, 970, 1225, 1616, 2348, 2486, 2573
BssECI	CCNNGG	6 cut(s)	844, 1485, 2333, 2450, 2533, 2582
BssMI	GATC	7 cut(s)	1264, 1828, 2270, 2280, 2563, 2587, 2758
BssNI	GRCGYC	1 cut(s)	696
BssT1I	CCWWGG	2 cut(s)	844, 2533
Bst2UI	CCWGG	6 cut(s)	11, 465, 1007, 1497, 2451, 2583
Bst6I	CTCTTC	3 cut(s)	48, 1240, 1602
BstACI	GRCGYC	1 cut(s)	696
BstAPI	GCANNNNNTGC	1 cut(s)	2246
BstAUI	TGTACA	2 cut(s)	742, 1581
BstBAI	YACGTR	1 cut(s)	181
BstC8I	GCNNGC	6 cut(s)	510, 736, 2132, 2417, 2742, 2751
BstDSI	CCRYGG	2 cut(s)	844, 2333
BstENI	CCTNNNNNAGG	2 cut(s)	23, 762
BstKTI	GATC	7 cut(s)	1267, 1831, 2273, 2283, 2566, 2590, 2761
BstMAI	GTCTC	5 cut(s)	71, 225, 890, 1461, 1992
BstMBI	GATC	7 cut(s)	1264, 1828, 2270, 2280, 2563, 2587, 2758
BstMWI	GCNNNNNNNGC	6 cut(s)	256, 518, 2246, 2344, 2746, 2750
BstNI	CCWGG	6 cut(s)	11, 465, 1007, 1497, 2451, 2583
BstNSI	RCATGY	1 cut(s)	2429
BstPAI	GACNNNNGTC	1 cut(s)	208
BstSCI	CCNGG	6 cut(s)	9, 463, 1005, 1495, 2449, 2581
BstSFI	CTRYAG	5 cut(s)	495, 540, 792, 1318, 2574
BstV1I	GCAGC	7 cut(s)	268, 509, 526, 1088, 1644, 2029, 2765
BstV2I	GAAGAC	2 cut(s)	272, 1857
BstX2I	RGATCY	3 cut(s)	1828, 2270, 2587
BstXI	CCANNNNNNTGG	1 cut(s)	1013
BstYI	RGATCY	3 cut(s)	1828, 2270, 2587
Bsu36I	CCTNAGG	1 cut(s)	1141
BsuI	GTATCC	3 cut(s)	451, 1314, 1467
BsuRI	GGCC	6 cut(s)	722, 1250, 2067, 2277, 2306, 2648
BtgI	CCRYGG	2 cut(s)	844, 2333
BtrI	CACGTC	1 cut(s)	2300
BtsIMutI	CAGTG	9 cut(s)	348, 952, 1860, 2112, 2177, 2400, 2493, 2562, 2566
BveI	ACCTGC	2 cut(s)	417, 2635
Cac8I	GCNNGC	6 cut(s)	510, 736, 2132, 2417, 2742, 2751
CaiI	CAGNNNCTG	2 cut(s)	109, 2644
Cfr13I	GGNCC	9 cut(s)	1223, 1274, 1786, 2049, 2066, 2081, 2189, 2454, 2647
CseI	GACGC	1 cut(s)	545
CsiI	ACCWGGT	1 cut(s)	9
Csp6I	GTAC	7 cut(s)	346, 413, 743, 901, 1379, 1582, 2524
CspCI	CAANNNNNGTGG	4 cut(s)	1650, 1685, 2101, 2136
CviQI	GTAC	7 cut(s)	346, 413, 743, 901, 1379, 1582, 2524
DpnI	GATC	7 cut(s)	1266, 1830, 2272, 2282, 2565, 2589, 2760
DpnII	GATC	7 cut(s)	1264, 1828, 2270, 2280, 2563, 2587, 2758
DrdI	GACNNNNNNGTC	2 cut(s)	521, 1427
DseDI	GACNNNNNNGTC	2 cut(s)	521, 1427
EaeI	YGGCCR	1 cut(s)	2275
Eam1104I	CTCTTC	3 cut(s)	48, 1240, 1602
EarI	CTCTTC	3 cut(s)	48, 1240, 1602
Eco130I	CCWWGG	2 cut(s)	844, 2533
Eco31I	GGTCTC	1 cut(s)	1992
Eco32I	GATATC	1 cut(s)	384
Eco47I	GGWCC	7 cut(s)	1223, 1274, 1786, 2049, 2081, 2189, 2454
Eco57I	CTGAAG	3 cut(s)	72, 1583, 2136
Eco81I	CCTNAGG	1 cut(s)	1141
Eco88I	CYCGRG	3 cut(s)	416, 1464, 2181
EcoNI	CCTNNNNNAGG	2 cut(s)	23, 762
EcoO109I	RGGNCCY	4 cut(s)	1786, 2081, 2189, 2454
EcoRII	CCWGG	6 cut(s)	9, 463, 1005, 1495, 2449, 2581
EcoRV	GATATC	1 cut(s)	384
EcoT14I	CCWWGG	2 cut(s)	844, 2533
EcoT22I	ATGCAT	2 cut(s)	670, 1336
ErhI	CCWWGG	2 cut(s)	844, 2533
Esp3I	CGTCTC	2 cut(s)	225, 890
FalI	AAGNNNNNCTT	6 cut(s)	673, 705, 2178, 2210, 2465, 2497
FaqI	GGGAC	6 cut(s)	216, 391, 1689, 1765, 2094, 2467
FauNDI	CATATG	2 cut(s)	306, 2054
FblI	GTMKAC	2 cut(s)	60, 1206
Fnu4HI	GCNGC	9 cut(s)	257, 498, 540, 720, 1077, 1191, 1658, 2043, 2754
Fsp4HI	GCNGC	9 cut(s)	257, 498, 540, 720, 1077, 1191, 1658, 2043, 2754
FspBI	CTAG	3 cut(s)	410, 1197, 2019
GluI	GCNGC	9 cut(s)	257, 498, 540, 720, 1077, 1191, 1658, 2043, 2754
HaeIII	GGCC	6 cut(s)	722, 1250, 2067, 2277, 2306, 2648
HapII	CCGG	1 cut(s)	911
HgaI	GACGC	1 cut(s)	545
Hin1I	GRCGYC	1 cut(s)	696
HincII	GTYRAC	3 cut(s)	628, 729, 2122
HindII	GTYRAC	3 cut(s)	628, 729, 2122
HindIII	AAGCTT	1 cut(s)	2128
HpaII	CCGG	1 cut(s)	911
HphI	GGTGA	4 cut(s)	77, 293, 1395, 1450
Hpy188I	TCNGA	7 cut(s)	231, 484, 1315, 1365, 1807, 1992, 2213
Hpy99I	CGWCG	2 cut(s)	2301, 2723
HpyAV	CCTTC	8 cut(s)	539, 1185, 1388, 1511, 1777, 1987, 2140, 2180
HpyCH4IV	ACGT	6 cut(s)	180, 234, 696, 809, 2299, 2674
HpyF10VI	GCNNNNNNNGC	6 cut(s)	256, 518, 2246, 2344, 2746, 2750
HpySE526I	ACGT	6 cut(s)	180, 234, 696, 809, 2299, 2674
Hsp92I	GRCGYC	1 cut(s)	696
KflI	GGGWCCC	1 cut(s)	2454
Kpn2I	TCCGGA	1 cut(s)	910
KpnI	GGTACC	1 cut(s)	2527
Kzo9I	GATC	7 cut(s)	1264, 1828, 2270, 2280, 2563, 2587, 2758
LmnI	GCTCC	4 cut(s)	1495, 1735, 2344, 2737
Lsp1109I	GCAGC	7 cut(s)	268, 509, 526, 1088, 1644, 2029, 2765
LweI	GCATC	8 cut(s)	88, 655, 1321, 1516, 2227, 2613, 2650, 2712
MabI	ACCWGGT	1 cut(s)	9
MaeI	CTAG	3 cut(s)	410, 1197, 2019
MaeII	ACGT	6 cut(s)	180, 234, 696, 809, 2299, 2674
MaeIII	GTNAC	4 cut(s)	1060, 1291, 1438, 2428
MalI	GATC	7 cut(s)	1266, 1830, 2272, 2282, 2565, 2589, 2760
MboI	GATC	7 cut(s)	1264, 1828, 2270, 2280, 2563, 2587, 2758
MfeI	CAATTG	1 cut(s)	603
MflI	RGATCY	3 cut(s)	1828, 2270, 2587
MhlI	GDGCHC	2 cut(s)	1740, 2182
MlsI	TGGCCA	1 cut(s)	2277
MluCI	AATT	8 cut(s)	475, 603, 650, 1052, 1298, 2005, 2229, 2711
MluNI	TGGCCA	1 cut(s)	2277
MlyI	GAGTC	5 cut(s)	198, 292, 1226, 1736, 1871
MmeI	TCCRAC	2 cut(s)	68, 1338
Mox20I	TGGCCA	1 cut(s)	2277
Mph1103I	ATGCAT	2 cut(s)	670, 1336
MroI	TCCGGA	1 cut(s)	910
MscI	TGGCCA	1 cut(s)	2277
MseI	TTAA	4 cut(s)	126, 390, 702, 837
MslI	CAYNNNNRTG	3 cut(s)	179, 281, 912
Msp20I	TGGCCA	1 cut(s)	2277
MspA1I	CMGCKG	3 cut(s)	50, 2435, 2756
MspI	CCGG	1 cut(s)	911
MspR9I	CCNGG	6 cut(s)	11, 465, 1007, 1497, 2451, 2583
MunI	CAATTG	1 cut(s)	603
MvaI	CCWGG	6 cut(s)	11, 465, 1007, 1497, 2451, 2583
MwoI	GCNNNNNNNGC	6 cut(s)	256, 518, 2246, 2344, 2746, 2750
NcoI	CCATGG	1 cut(s)	844
NdeI	CATATG	2 cut(s)	306, 2054
NdeII	GATC	7 cut(s)	1264, 1828, 2270, 2280, 2563, 2587, 2758
NmeAIII	GCCGAG	1 cut(s)	1510
NmuCI	GTSAC	3 cut(s)	1060, 1291, 1438
NsiI	ATGCAT	2 cut(s)	670, 1336
NspI	RCATGY	1 cut(s)	2429
OliI	CACNNNNGTG	1 cut(s)	179
PaeR7I	CTCGAG	3 cut(s)	416, 1464, 2181
PciI	ACATGT	1 cut(s)	2425
PcsI	WCGNNNNNNNCGW	1 cut(s)	815
PfeI	GAWTC	7 cut(s)	167, 320, 1360, 1772, 2150, 2495, 2635
PkrI	GCNGC	9 cut(s)	258, 499, 541, 721, 1078, 1192, 1659, 2044, 2755
PleI	GAGTC	5 cut(s)	198, 292, 1226, 1735, 1870
PpsI	GAGTC	5 cut(s)	198, 292, 1226, 1735, 1870
Ppu21I	YACGTR	1 cut(s)	181
PpuMI	RGGWCCY	4 cut(s)	1786, 2081, 2189, 2454
PscI	ACATGT	1 cut(s)	2425
PshAI	GACNNNNGTC	1 cut(s)	208
PshBI	ATTAAT	1 cut(s)	702
Psp1406I	AACGTT	1 cut(s)	234
Psp5II	RGGWCCY	4 cut(s)	1786, 2081, 2189, 2454
Psp6I	CCWGG	6 cut(s)	9, 463, 1005, 1495, 2449, 2581
PspGI	CCWGG	6 cut(s)	9, 463, 1005, 1495, 2449, 2581
PspPI	GGNCC	9 cut(s)	1223, 1274, 1786, 2049, 2066, 2081, 2189, 2454, 2647
PspPPI	RGGWCCY	4 cut(s)	1786, 2081, 2189, 2454
PspXI	VCTCGAGB	1 cut(s)	416
PstI	CTGCAG	3 cut(s)	499, 544, 796
PstNI	CAGNNNCTG	2 cut(s)	109, 2644
PsuI	RGATCY	3 cut(s)	1828, 2270, 2587
PvuII	CAGCTG	2 cut(s)	2435, 2756
RsaI	GTAC	7 cut(s)	347, 414, 744, 902, 1380, 1583, 2525
RsaNI	GTAC	7 cut(s)	346, 413, 743, 901, 1379, 1582, 2524
RseI	CAYNNNNRTG	3 cut(s)	179, 281, 912
SaqAI	TTAA	4 cut(s)	126, 390, 702, 837
SatI	GCNGC	9 cut(s)	257, 498, 540, 720, 1077, 1191, 1658, 2043, 2754
Sau3AI	GATC	7 cut(s)	1264, 1828, 2270, 2280, 2563, 2587, 2758
Sau96I	GGNCC	9 cut(s)	1223, 1274, 1786, 2049, 2066, 2081, 2189, 2454, 2647
ScaI	AGTACT	1 cut(s)	414
SchI	GAGTC	5 cut(s)	198, 292, 1226, 1736, 1871
ScrFI	CCNGG	6 cut(s)	11, 465, 1007, 1497, 2451, 2583
SduI	GDGCHC	2 cut(s)	1740, 2182
SexAI	ACCWGGT	1 cut(s)	9
SfaNI	GCATC	8 cut(s)	88, 655, 1321, 1516, 2227, 2613, 2650, 2712
SfcI	CTRYAG	5 cut(s)	495, 540, 792, 1318, 2574
Sfr274I	CTCGAG	3 cut(s)	416, 1464, 2181
SinI	GGWCC	7 cut(s)	1223, 1274, 1786, 2049, 2081, 2189, 2454
SlaI	CTCGAG	3 cut(s)	416, 1464, 2181
SmiMI	CAYNNNNRTG	3 cut(s)	179, 281, 912
SmlI	CTYRAG	4 cut(s)	416, 1464, 2181, 2518
SmoI	CTYRAG	4 cut(s)	416, 1464, 2181, 2518
Sse9I	AATT	8 cut(s)	475, 603, 650, 1052, 1298, 2005, 2229, 2711
SsiI	CCGC	4 cut(s)	48, 719, 1191, 1826
SspI	AATATT	1 cut(s)	1565
SspMI	CTAG	3 cut(s)	410, 1197, 2019
StyD4I	CCNGG	6 cut(s)	9, 463, 1005, 1495, 2449, 2581
StyI	CCWWGG	2 cut(s)	844, 2533
TaiI	ACGT	6 cut(s)	183, 237, 699, 812, 2302, 2677
TaqI	TCGA	9 cut(s)	212, 417, 1238, 1465, 2153, 2182, 2296, 2562, 2718
TasI	AATT	8 cut(s)	475, 603, 650, 1052, 1298, 2005, 2229, 2711
TatI	WGTACW	4 cut(s)	412, 742, 900, 1581
TauI	GCSGC	2 cut(s)	722, 1193
TfiI	GAWTC	7 cut(s)	167, 320, 1360, 1772, 2150, 2495, 2635
Tru1I	TTAA	4 cut(s)	126, 390, 702, 837
Tru9I	TTAA	4 cut(s)	126, 390, 702, 837
TscAI	CASTG	9 cut(s)	355, 952, 1860, 2119, 2177, 2407, 2493, 2562, 2573
TseFI	GTSAC	3 cut(s)	1060, 1291, 1438
TseI	GCWGC	7 cut(s)	256, 497, 539, 1076, 1657, 2042, 2753
Tsp45I	GTSAC	3 cut(s)	1060, 1291, 1438
TspGWI	ACGGA	2 cut(s)	875, 1580
TspRI	CASTG	9 cut(s)	355, 952, 1860, 2119, 2177, 2407, 2493, 2562, 2573
VpaK11BI	GGWCC	7 cut(s)	1223, 1274, 1786, 2049, 2081, 2189, 2454
VspI	ATTAAT	1 cut(s)	702
XagI	CCTNNNNNAGG	2 cut(s)	23, 762
XapI	RAATTY	2 cut(s)	1052, 2711
XceI	RCATGY	1 cut(s)	2429
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1217
XhoI	CTCGAG	3 cut(s)	416, 1464, 2181
XmiI	GTMKAC	2 cut(s)	60, 1206
XspI	CTAG	3 cut(s)	410, 1197, 2019
ZraI	GACGTC	1 cut(s)	697
ZrmI	AGTACT	1 cut(s)	414
Zsp2I	ATGCAT	2 cut(s)	670, 1336

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.