pycom15g18320

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002889
Physical Location & Seq
Forward (+)
13005707 .. 13011001
5295 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2514 bp
ATGAAATGCAGTGGAAAGTGGGCCTCTATTTCCCTTTGCACATTTGCAATATTCAAAAGCTTATATAAAGCAGTTGCGGGAGGTTCCCGTTCTCTGCCCAACCAAACCGAAAACTCTCTCACCCATCAAACCCATTGCACTTCTCAACTCTGCCACTCTCTCTCGCTGTCTCTTTCCAAAATATCAAAAGCACCAAACAAAAGAAACCAAATTCAAATCCCAATACAAAATATATATTTCAAGTTTTTGGTACAAACAAAGCAAGTATATTTATATATAAAGGCCATTGCTTTTGAGCGTTTCAGAAGCAGGGGAAAAATGGGAGTTGGAATTCAAACAATGTGGAGCATTCTGCTATTGTTTTCCTGCATTTTTTCTGCAGCTTCGGCTTCTGTGAGTTACGACCACAAGGCTATAATAATTAATGGGCAGAAAAGGATTTTAATTTCTGGCTCCATTCACTATCCCAGAAGCACTCCTGAGATGTGGCCGGATTTAATTCAGAAGGCCAAGGATGGAGGCTTGGATGTTATACAGACCTATGTGTTTTGGAATGGCCACGAACCTTCTCCGGGAAAATATTATTTCGAGGACAGATATGATTTGGTCAAGTTCATCAAGCTGGTGCAACAAGCAGGCCTATTTGTTAATCTCCGGATTGGCCCTTATGTTTGCGCTGAATGGAACTTCGGGGGATTCCCAGTTTGGCTGAAATATGTCCCTGGAATCGCTTTTCGAACGGACAATGAGCCTTTCAAGGCGGCAATGCAAAAATTTACAGAGAAGATTGTCAGCATGATGAAGGCAGAGAAGCTGTTTCAAACTCAAGGAGGTCCTATAATTCTCTCTCAGATAGAAAATGAATTTGGACCTGTGGAATGGGAAATTGGTGCACCTGGAAAAGCTTACACCAAATGGGCAGCTCAGATGGCTGTAGGTCTAGACACTGGAGTTCCATGGATTATGTGCAAGCAAGAGGATGCCCCCGATCCCGTTATTGACACTTGCAATGGTTTCTACTGTGAGAATTTCAAGCCAAATAAGGACTATAAGCCCAAAATGTGGACAGAAGTCTGGACTGGTTGGTATACAGAATTCGGTGGGGCAGTTCCCACTAGACCTGCAGAAGATGTGGCATTTTCAGTTGCTAGGTTCATACAAAGCGGCGGTTCGTTTTTGAACTATTACATGTACCACGGAGGAACGAATTTTGGCCGAACAGCTGGAGGTCCCTTCATGGCCACTAGCTATGACTATGACGCCCCCTTAGACGAATATGGACTACTCCGGGAACCAAAGTGGGGACATTTGAGAGATCTGCACAAAGCCATTAAATCATGCGAGTCTGCTTTAGTGTCCGTTGATCCTTCAGTGACTAAACTCGGAAGTAATCAAGAGGCTCATGTATTCAAATCAGAGTCGGATTGCGCTGCATTCCTCGCAAATTATGACGCAAAATACTCTGTTAAAGTGAGCTTTGGAGGCGGGCAGTATGACCTGCCGCCATGGTCCATCAGCATTCTTCCGGACTGCAAAACCGAAGTTTACAACACTGCAAAGGTTGCTTCGCAAAGCTCGCAAGTTCAGATGACACCAGTACATAGTGGATTTCCTTGGCAGTCATTCATCGAAGAAACCACCTCTTCTGATGAGGCCGATACAACTACATTGGACGGGTTGTATGAGCAAATAAATATCACTAGGGATACTACAGACTACTTGTGGTACATGACAGATATCACAATAGGTTCTGATGAAGCATTTCTAAAGAACGGAAAGTCCCCACTTCTTACGATCTCTTCAGCAGGTCATGCCTTGAATGTTTTCATCAATGGTCAGCTCTCAGGAACCGTGTATGGGTCGTTGGAGAATCCTAAATTATCATTCAGTCAAAACGTGAACCTGAGATCTGGCATCAACAAGCTTGCATTGCTTAGCATTTCCGTTGGTCTGCCGAATGTTGGTACTCACTTTGAGACATGGAACACGGGAGTTCTTGGCCCGATCACATTGAAAGGTCTGAATTCAGGAACATGGGACATGTCAGGGTGGAAATGGACATACAAGACTGGTCTGAAAGGTGAAGCTTTAGGCCTCCATACTGTTACTGGGAGTTCTTCTGTTGAATGGGTAGAAGGACCATCGATGGCTAAAAAACAACCCCTTACGTGGTACAAGGCTACTTTTAATGCACCACCAGGTGATGCTCCATTAGCTTTAGATATGGGAAGCATGGGAAAAGGTCAGATATGGATAAATGGACAGAGTGTGGGACGCCACTGGCCTGGATACACTGCACGCGGCAGCTGTGGCGATTGTTCTTATGCCGGAACTTATGATGATAAGAAATGCAGAACTCATTGCGGCGAGCCCTCTCAGAGATGGTACCACATTCCTCGATCGTGGTTGACCCCGACTGGAAATCTTTTGGTAGTGTTCGAAGAATGGGGCGGTGATCCGTCAGGGATTTCGTTGGTTGAAAGAGGTACAGCCCTCGACGCGAAGAAGCTCTAG

Protein Analysis

838

Amino Acids

92.81

Weight (kDa)

8.14

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 139 - 443 1e-119 Glycosyl hydrolases family 35
GHD PF17834 466 - 521 1.3e-22 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 573 - 640 2.5e-07 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 722 - 808 5.6e-25 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1129, 1506, 1796
Acc65I GGTACC 1 cut(s) 2385
AccB1I GGYRCC 1 cut(s) 2385
AccB7I CCANNNNNTGG 1 cut(s) 1062
AccI GTMKAC 1 cut(s) 1088
AccII CGCG 2 cut(s) 2301, 2501
AccIII TCCGGA 2 cut(s) 654, 1525
AciI CCGC 9 cut(s) 77, 761, 1164, 1167, 1485, 1502, 2301, 2364, 2451
AclWI GGATC 3 cut(s) 983, 1358, 2450
AcoI YGGCCR 4 cut(s) 488, 556, 1213, 1239
AcsI RAATTY 8 cut(s) 210, 330, 773, 863, 1027, 1094, 1207, 2023
AcuI CTGAAG 2 cut(s) 1353, 1785
AcyI GRCGYC 2 cut(s) 1260, 2275
AdeI CACNNNGTG 1 cut(s) 2201
AfaI GTAC 8 cut(s) 252, 1193, 1599, 1727, 1966, 2174, 2387, 2488
AfiI CCNNNNNNNGG 6 cut(s) 572, 1062, 1301, 1857, 1961, 2169
AflIII ACRYGT 2 cut(s) 1188, 2040
AjnI CCWGG 4 cut(s) 721, 895, 2197, 2284
AjuI GAANNNNNNNTTGG 4 cut(s) 849, 870, 881, 902
AloI GAACNNNNNNTCC 2 cut(s) 1763, 1795
Alw21I GWGCWC 1 cut(s) 895
Alw26I GTCTC 2 cut(s) 174, 1970
Alw44I GTGCAC 1 cut(s) 891
AlwI GGATC 3 cut(s) 983, 1358, 2450
Aor13HI TCCGGA 2 cut(s) 654, 1525
ApaLI GTGCAC 1 cut(s) 891
ApeKI GCWGC 4 cut(s) 380, 920, 1430, 2304
ApoI RAATTY 8 cut(s) 210, 330, 773, 863, 1027, 1094, 1207, 2023
AseI ATTAAT 1 cut(s) 423
Asp700I GAANNNNTTC 2 cut(s) 1761, 1823
Asp718I GGTACC 1 cut(s) 2385
AspLEI GCGC 2 cut(s) 677, 1430
AspS9I GGNCC 8 cut(s) 21, 662, 833, 869, 1229, 1509, 2000, 2138
AsuC2I CCSGG 2 cut(s) 573, 1289
AsuHPI GGTGA 4 cut(s) 112, 2093, 2213, 2465
AsuII TTCGAA 2 cut(s) 736, 2439
AvaII GGWCC 5 cut(s) 833, 869, 1229, 1509, 2138
BaeGI GKGCMC 1 cut(s) 895
BalI TGGCCA 2 cut(s) 558, 1241
BanI GGYRCC 1 cut(s) 2385
BanII GRGCYC 1 cut(s) 2373
Bbv12I GWGCWC 1 cut(s) 895
BbvI GCAGC 4 cut(s) 392, 932, 1417, 2316
BccI CCATC 7 cut(s) 132, 509, 922, 1520, 2140, 2149, 2376
BciT130I CCWGG 4 cut(s) 723, 897, 2199, 2286
BciVI GTATCC 2 cut(s) 1699, 2282
BcnI CCSGG 2 cut(s) 573, 1289
BcoDI GTCTC 2 cut(s) 174, 1970
BfaI CTAG 6 cut(s) 941, 1116, 1149, 1245, 1701, 2512
BfmI CTRYAG 4 cut(s) 378, 933, 1122, 1710
BfuAI ACCTGC 3 cut(s) 1129, 1506, 1796
BfuI GTATCC 2 cut(s) 1699, 2282
BglII AGATCT 2 cut(s) 1315, 1907
BisI GCNGC 9 cut(s) 381, 762, 921, 1165, 1431, 1502, 2302, 2305, 2365
BlpI GCTNAGC 1 cut(s) 1934
BlsI GCNGC 9 cut(s) 382, 763, 922, 1166, 1432, 1503, 2303, 2306, 2366
Bme1390I CCNGG 6 cut(s) 573, 723, 897, 1289, 2199, 2286
Bme18I GGWCC 5 cut(s) 833, 869, 1229, 1509, 2138
BmgT120I GGNCC 8 cut(s) 21, 662, 833, 869, 1229, 1509, 2000, 2138
BmiI GGNNCC 6 cut(s) 85, 454, 1231, 1293, 1849, 2387
BmrFI CCNGG 6 cut(s) 573, 723, 897, 1289, 2199, 2286
BmrI ACTGGG 2 cut(s) 695, 2118
BmsI GCATC 3 cut(s) 970, 1923, 2194
BmuI ACTGGG 2 cut(s) 695, 2118
BoxI GACNNNNGTC 1 cut(s) 1070
BpmI CTGGAG 2 cut(s) 969, 1245
Bpu1102I GCTNAGC 1 cut(s) 1934
Bpu14I TTCGAA 2 cut(s) 736, 2439
BpuEI CTTGAG 1 cut(s) 810
BpuMI CCSGG 2 cut(s) 573, 1289
Bsa29I ATCGAT 1 cut(s) 2144
BsaAI YACGTR 1 cut(s) 2169
BsaHI GRCGYC 2 cut(s) 1260, 2275
BsaJI CCNNGG 6 cut(s) 510, 721, 956, 1195, 1505, 1613
BsaWI WCCGGW 2 cut(s) 654, 1525
Bsc4I CCNNNNNNNGG 6 cut(s) 572, 1062, 1301, 1857, 1961, 2169
Bse1I ACTGG 8 cut(s) 701, 952, 1084, 1595, 2074, 2113, 2285, 2422
Bse3DI GCAATG 6 cut(s) 133, 285, 771, 1015, 1928, 2359
BseAI TCCGGA 2 cut(s) 654, 1525
BseBI CCWGG 4 cut(s) 723, 897, 2199, 2286
BseCI ATCGAT 1 cut(s) 2144
BseDI CCNNGG 6 cut(s) 510, 721, 956, 1195, 1505, 1613
BseGI GGATG 3 cut(s) 520, 532, 985
BseLI CCNNNNNNNGG 6 cut(s) 572, 1062, 1301, 1857, 1961, 2169
BseMI GCAATG 6 cut(s) 133, 285, 771, 1015, 1928, 2359
BseMII CTCAG 6 cut(s) 471, 863, 938, 1857, 1895, 2390
BseNI ACTGG 8 cut(s) 701, 952, 1084, 1595, 2074, 2113, 2285, 2422
BseSI GKGCMC 1 cut(s) 895
BseXI GCAGC 4 cut(s) 392, 932, 1417, 2316
BsgI GTGCAG 2 cut(s) 1304, 2280
Bsh1236I CGCG 2 cut(s) 2301, 2501
Bsh1285I CGRYCG 1 cut(s) 2402
BshNI GGYRCC 1 cut(s) 2385
BshVI ATCGAT 1 cut(s) 2144
BsiEI CGRYCG 1 cut(s) 2402
BsiHKAI GWGCWC 1 cut(s) 895
BsiSI CCGG 6 cut(s) 491, 572, 655, 1288, 1526, 2328
BslFI GGGAC 6 cut(s) 704, 1215, 1317, 1765, 2051, 2286
BslI CCNNNNNNNGG 6 cut(s) 572, 1062, 1301, 1857, 1961, 2169
BsmAI GTCTC 2 cut(s) 174, 1970
BsmFI GGGAC 6 cut(s) 704, 1215, 1317, 1765, 2051, 2286
BsmI GAATGC 3 cut(s) 348, 1433, 1518
Bsp119I TTCGAA 2 cut(s) 736, 2439
Bsp1286I GDGCHC 2 cut(s) 895, 2373
Bsp13I TCCGGA 2 cut(s) 654, 1525
Bsp143I GATC 8 cut(s) 988, 1315, 1363, 1794, 1907, 2004, 2399, 2455
Bsp1720I GCTNAGC 1 cut(s) 1934
Bsp19I CCATGG 2 cut(s) 956, 1505
BspACI CCGC 9 cut(s) 77, 761, 1164, 1167, 1485, 1502, 2301, 2364, 2451
BspCNI CTCAG 6 cut(s) 472, 862, 937, 1856, 1896, 2389
BspDI ATCGAT 1 cut(s) 2144
BspEI TCCGGA 2 cut(s) 654, 1525
BspFNI CGCG 2 cut(s) 2301, 2501
BspLI GGNNCC 6 cut(s) 85, 454, 1231, 1293, 1849, 2387
BspMAI CTGCAG 2 cut(s) 382, 1126
BspMI ACCTGC 3 cut(s) 1129, 1506, 1796
BspPI GGATC 3 cut(s) 983, 1358, 2450
BspT104I TTCGAA 2 cut(s) 736, 2439
BspT107I GGYRCC 1 cut(s) 2385
BsrDI GCAATG 6 cut(s) 133, 285, 771, 1015, 1928, 2359
BsrI ACTGG 8 cut(s) 701, 952, 1084, 1595, 2074, 2113, 2285, 2422
BssECI CCNNGG 6 cut(s) 510, 721, 956, 1195, 1505, 1613
BssMI GATC 8 cut(s) 988, 1315, 1363, 1794, 1907, 2004, 2399, 2455
BssNAI GTATAC 1 cut(s) 1089
BssNI GRCGYC 2 cut(s) 1260, 2275
BssT1I CCWWGG 4 cut(s) 510, 956, 1505, 1613
Bst1107I GTATAC 1 cut(s) 1089
Bst2UI CCWGG 4 cut(s) 723, 897, 2199, 2286
Bst4CI ACNGT 3 cut(s) 1022, 1852, 2104
Bst6I CTCTTC 2 cut(s) 1648, 1804
BstACI GRCGYC 2 cut(s) 1260, 2275
BstAPI GCANNNNNTGC 2 cut(s) 1562, 1811
BstBAI YACGTR 1 cut(s) 2169
BstBI TTCGAA 2 cut(s) 736, 2439
BstC8I GCNNGC 7 cut(s) 637, 971, 1487, 1577, 1926, 2299, 2369
BstDEI CTNAG 8 cut(s) 480, 849, 924, 1267, 1843, 1904, 1934, 2376
BstDSI CCRYGG 3 cut(s) 956, 1195, 1505
BstF5I GGATG 3 cut(s) 520, 532, 985
BstFNI CGCG 2 cut(s) 2301, 2501
BstHHI GCGC 2 cut(s) 677, 1430
BstKTI GATC 8 cut(s) 991, 1318, 1366, 1797, 1910, 2007, 2402, 2458
BstMAI GTCTC 2 cut(s) 174, 1970
BstMBI GATC 8 cut(s) 988, 1315, 1363, 1794, 1907, 2004, 2399, 2455
BstMCI CGRYCG 1 cut(s) 2402
BstNI CCWGG 4 cut(s) 723, 897, 2199, 2286
BstNSI RCATGY 2 cut(s) 1192, 2044
BstPAI GACNNNNGTC 1 cut(s) 1070
BstSCI CCNGG 6 cut(s) 571, 721, 895, 1287, 2197, 2284
BstSFI CTRYAG 4 cut(s) 378, 933, 1122, 1710
BstSLI GKGCMC 1 cut(s) 895
BstUI CGCG 2 cut(s) 2301, 2501
BstV1I GCAGC 4 cut(s) 392, 932, 1417, 2316
BstX2I RGATCY 2 cut(s) 1315, 1907
BstXI CCANNNNNNTGG 1 cut(s) 2285
BstYI RGATCY 2 cut(s) 1315, 1907
BstZ17I GTATAC 1 cut(s) 1089
Bsu15I ATCGAT 1 cut(s) 2144
BsuI GTATCC 2 cut(s) 1699, 2282
BsuTUI ATCGAT 1 cut(s) 2144
BtgI CCRYGG 3 cut(s) 956, 1195, 1505
BtsCI GGATG 3 cut(s) 520, 532, 985
BtsI GCAGTG 3 cut(s) 16, 1551, 2292
BtsIMutI CAGTG 6 cut(s) 16, 945, 1377, 1551, 2278, 2292
BveI ACCTGC 3 cut(s) 1129, 1506, 1796
Cac8I GCNNGC 7 cut(s) 637, 971, 1487, 1577, 1926, 2299, 2369
CfoI GCGC 2 cut(s) 677, 1430
Cfr13I GGNCC 8 cut(s) 21, 662, 833, 869, 1229, 1509, 2000, 2138
ClaI ATCGAT 1 cut(s) 2144
CseI GACGC 4 cut(s) 1268, 1460, 2283, 2507
CsiI ACCWGGT 1 cut(s) 2197
Csp6I GTAC 8 cut(s) 251, 1192, 1598, 1726, 1965, 2173, 2386, 2487
CspCI CAANNNNNGTGG 2 cut(s) 323, 358
CviQI GTAC 8 cut(s) 251, 1192, 1598, 1726, 1965, 2173, 2386, 2487
DdeI CTNAG 8 cut(s) 480, 849, 924, 1267, 1843, 1904, 1934, 2376
DpnI GATC 8 cut(s) 990, 1317, 1365, 1796, 1909, 2006, 2401, 2457
DpnII GATC 8 cut(s) 988, 1315, 1363, 1794, 1907, 2004, 2399, 2455
DraIII CACNNNGTG 1 cut(s) 2201
EaeI YGGCCR 4 cut(s) 488, 556, 1213, 1239
Eam1104I CTCTTC 2 cut(s) 1648, 1804
EarI CTCTTC 2 cut(s) 1648, 1804
Eco130I CCWWGG 4 cut(s) 510, 956, 1505, 1613
Eco147I AGGCCT 2 cut(s) 639, 2094
Eco24I GRGCYC 1 cut(s) 2373
Eco32I GATATC 1 cut(s) 1738
Eco47I GGWCC 5 cut(s) 833, 869, 1229, 1509, 2138
Eco57I CTGAAG 2 cut(s) 1353, 1785
EcoO109I RGGNCCY 2 cut(s) 833, 1229
EcoRI GAATTC 3 cut(s) 330, 1094, 2023
EcoRII CCWGG 4 cut(s) 721, 895, 2197, 2284
EcoRV GATATC 1 cut(s) 1738
EcoT14I CCWWGG 4 cut(s) 510, 956, 1505, 1613
EcoT38I GRGCYC 1 cut(s) 2373
ErhI CCWWGG 4 cut(s) 510, 956, 1505, 1613
FaqI GGGAC 6 cut(s) 704, 1215, 1317, 1765, 2051, 2286
FauI CCCGC 2 cut(s) 70, 1478
FblI GTMKAC 1 cut(s) 1088
Fnu4HI GCNGC 9 cut(s) 381, 762, 921, 1165, 1431, 1502, 2302, 2305, 2365
FokI GGATG 3 cut(s) 527, 539, 992
FriOI GRGCYC 1 cut(s) 2373
Fsp4HI GCNGC 9 cut(s) 381, 762, 921, 1165, 1431, 1502, 2302, 2305, 2365
FspBI CTAG 6 cut(s) 941, 1116, 1149, 1245, 1701, 2512
GlaI GCGC 2 cut(s) 676, 1429
GluI GCNGC 9 cut(s) 381, 762, 921, 1165, 1431, 1502, 2302, 2305, 2365
GsuI CTGGAG 2 cut(s) 969, 1245
HapII CCGG 6 cut(s) 491, 572, 655, 1288, 1526, 2328
HgaI GACGC 4 cut(s) 1268, 1460, 2283, 2507
HhaI GCGC 2 cut(s) 677, 1430
Hin1I GRCGYC 2 cut(s) 1260, 2275
Hin6I GCGC 2 cut(s) 675, 1428
HinP1I GCGC 2 cut(s) 675, 1428
HincII GTYRAC 1 cut(s) 2409
HindII GTYRAC 1 cut(s) 2409
HindIII AAGCTT 4 cut(s) 58, 903, 1922, 2085
HinfI GANTC 5 cut(s) 696, 726, 1343, 1418, 1870
HpaII CCGG 6 cut(s) 491, 572, 655, 1288, 1526, 2328
HphI GGTGA 4 cut(s) 112, 2093, 2213, 2465
Hpy166II GTNNAC 6 cut(s) 893, 1065, 1089, 1546, 1900, 2409
Hpy188III TCNNGA 8 cut(s) 479, 655, 941, 1075, 1394, 1526, 1845, 2028
Hpy8I GTNNAC 6 cut(s) 893, 1065, 1089, 1546, 1900, 2409
Hpy99I CGWCG 1 cut(s) 2501
HpyAV CCTTC 6 cut(s) 499, 576, 796, 1243, 1377, 2129
HpyCH4III ACNGT 3 cut(s) 1022, 1852, 2104
HpyCH4IV ACGT 2 cut(s) 1896, 2168
HpyF3I CTNAG 8 cut(s) 480, 849, 924, 1267, 1843, 1904, 1934, 2376
HpySE526I ACGT 2 cut(s) 1896, 2168
Hsp92I GRCGYC 2 cut(s) 1260, 2275
HspAI GCGC 2 cut(s) 675, 1428
Kpn2I TCCGGA 2 cut(s) 654, 1525
KpnI GGTACC 1 cut(s) 2389
Kzo9I GATC 8 cut(s) 988, 1315, 1363, 1794, 1907, 2004, 2399, 2455
LmnI GCTCC 3 cut(s) 345, 458, 2212
Lsp1109I GCAGC 4 cut(s) 392, 932, 1417, 2316
LweI GCATC 3 cut(s) 970, 1923, 2194
MabI ACCWGGT 1 cut(s) 2197
MaeI CTAG 6 cut(s) 941, 1116, 1149, 1245, 1701, 2512
MaeII ACGT 2 cut(s) 1896, 2168
MaeIII GTNAC 3 cut(s) 398, 1372, 2104
MalI GATC 8 cut(s) 990, 1317, 1365, 1796, 1909, 2006, 2401, 2457
MboI GATC 8 cut(s) 988, 1315, 1363, 1794, 1907, 2004, 2399, 2455
MboII GAAGA 8 cut(s) 796, 1139, 1514, 1635, 1643, 1791, 2109, 2453
MflI RGATCY 2 cut(s) 1315, 1907
MhlI GDGCHC 2 cut(s) 895, 2373
MlsI TGGCCA 2 cut(s) 558, 1241
MluNI TGGCCA 2 cut(s) 558, 1241
MlyI GAGTC 2 cut(s) 1352, 1427
MmeI TCCRAC 3 cut(s) 307, 1401, 1845
Mox20I TGGCCA 2 cut(s) 558, 1241
MroI TCCGGA 2 cut(s) 654, 1525
MroXI GAANNNNTTC 2 cut(s) 1761, 1823
MscI TGGCCA 2 cut(s) 558, 1241
MseI TTAA 7 cut(s) 423, 443, 497, 648, 1332, 1467, 2187
Msp20I TGGCCA 2 cut(s) 558, 1241
MspA1I CMGCKG 2 cut(s) 1223, 2307
MspI CCGG 6 cut(s) 491, 572, 655, 1288, 1526, 2328
MspR9I CCNGG 6 cut(s) 573, 723, 897, 1289, 2199, 2286
Mva1269I GAATGC 3 cut(s) 348, 1433, 1518
MvaI CCWGG 4 cut(s) 723, 897, 2199, 2286
MvnI CGCG 2 cut(s) 2301, 2501
NciI CCSGG 2 cut(s) 573, 1289
NcoI CCATGG 2 cut(s) 956, 1505
NdeII GATC 8 cut(s) 988, 1315, 1363, 1794, 1907, 2004, 2399, 2455
NlaIV GGNNCC 6 cut(s) 85, 454, 1231, 1293, 1849, 2387
NmuCI GTSAC 1 cut(s) 1372
NspI RCATGY 2 cut(s) 1192, 2044
NspV TTCGAA 2 cut(s) 736, 2439
PceI AGGCCT 2 cut(s) 639, 2094
PciI ACATGT 2 cut(s) 1188, 2040
PctI GAATGC 3 cut(s) 348, 1433, 1518
PdmI GAANNNNTTC 2 cut(s) 1761, 1823
PfeI GAWTC 3 cut(s) 696, 726, 1870
PflMI CCANNNNNTGG 1 cut(s) 1062
PfoI TCCNGGA 2 cut(s) 571, 1287
PkrI GCNGC 9 cut(s) 382, 763, 922, 1166, 1432, 1503, 2303, 2306, 2366
Ple19I CGATCG 1 cut(s) 2402
PleI GAGTC 2 cut(s) 1351, 1426
PpsI GAGTC 2 cut(s) 1351, 1426
Ppu21I YACGTR 1 cut(s) 2169
PpuMI RGGWCCY 2 cut(s) 833, 1229
PscI ACATGT 2 cut(s) 1188, 2040
PshAI GACNNNNGTC 1 cut(s) 1070
PshBI ATTAAT 1 cut(s) 423
Psp5II RGGWCCY 2 cut(s) 833, 1229
Psp6I CCWGG 4 cut(s) 721, 895, 2197, 2284
PspGI CCWGG 4 cut(s) 721, 895, 2197, 2284
PspN4I GGNNCC 6 cut(s) 85, 454, 1231, 1293, 1849, 2387
PspPI GGNCC 8 cut(s) 21, 662, 833, 869, 1229, 1509, 2000, 2138
PspPPI RGGWCCY 2 cut(s) 833, 1229
PstI CTGCAG 2 cut(s) 382, 1126
PsuI RGATCY 2 cut(s) 1315, 1907
PvuI CGATCG 1 cut(s) 2402
PvuII CAGCTG 2 cut(s) 1223, 2307
RsaI GTAC 8 cut(s) 252, 1193, 1599, 1727, 1966, 2174, 2387, 2488
RsaNI GTAC 8 cut(s) 251, 1192, 1598, 1726, 1965, 2173, 2386, 2487
SaqAI TTAA 7 cut(s) 423, 443, 497, 648, 1332, 1467, 2187
SatI GCNGC 9 cut(s) 381, 762, 921, 1165, 1431, 1502, 2302, 2305, 2365
Sau3AI GATC 8 cut(s) 988, 1315, 1363, 1794, 1907, 2004, 2399, 2455
Sau96I GGNCC 8 cut(s) 21, 662, 833, 869, 1229, 1509, 2000, 2138
SchI GAGTC 2 cut(s) 1352, 1427
ScrFI CCNGG 6 cut(s) 573, 723, 897, 1289, 2199, 2286
SduI GDGCHC 2 cut(s) 895, 2373
SexAI ACCWGGT 1 cut(s) 2197
SfaNI GCATC 3 cut(s) 970, 1923, 2194
SfcI CTRYAG 4 cut(s) 378, 933, 1122, 1710
SfuI TTCGAA 2 cut(s) 736, 2439
SinI GGWCC 5 cut(s) 833, 869, 1229, 1509, 2138
SmlI CTYRAG 1 cut(s) 825
SmoI CTYRAG 1 cut(s) 825
SseBI AGGCCT 2 cut(s) 639, 2094
SsiI CCGC 9 cut(s) 77, 761, 1164, 1167, 1485, 1502, 2301, 2364, 2451
SspI AATATT 2 cut(s) 51, 581
SspMI CTAG 6 cut(s) 941, 1116, 1149, 1245, 1701, 2512
StuI AGGCCT 2 cut(s) 639, 2094
StyD4I CCNGG 6 cut(s) 571, 721, 895, 1287, 2197, 2284
StyI CCWWGG 4 cut(s) 510, 956, 1505, 1613
TaaI ACNGT 3 cut(s) 1022, 1852, 2104
TaiI ACGT 2 cut(s) 1899, 2171
TaqI TCGA 7 cut(s) 588, 736, 1629, 2144, 2398, 2439, 2496
TatI WGTACW 1 cut(s) 1597
TauI GCSGC 5 cut(s) 764, 1167, 1504, 2304, 2367
TfiI GAWTC 3 cut(s) 696, 726, 1870
Tru1I TTAA 7 cut(s) 423, 443, 497, 648, 1332, 1467, 2187
Tru9I TTAA 7 cut(s) 423, 443, 497, 648, 1332, 1467, 2187
TscAI CASTG 6 cut(s) 16, 952, 1377, 1558, 2285, 2299
TseFI GTSAC 1 cut(s) 1372
TseI GCWGC 4 cut(s) 380, 920, 1430, 2304
Tsp45I GTSAC 1 cut(s) 1372
TspDTI ATGAA 9 cut(s) 17, 604, 815, 876, 1144, 1225, 1615, 1770, 1816
TspGWI ACGGA 6 cut(s) 755, 1212, 1348, 1788, 1933, 2448
TspRI CASTG 6 cut(s) 16, 952, 1377, 1558, 2285, 2299
Van91I CCANNNNNTGG 1 cut(s) 1062
VneI GTGCAC 1 cut(s) 891
VpaK11BI GGWCC 5 cut(s) 833, 869, 1229, 1509, 2138
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 8 cut(s) 210, 330, 773, 863, 1027, 1094, 1207, 2023
XbaI TCTAGA 1 cut(s) 940
XceI RCATGY 2 cut(s) 1192, 2044
XcmI CCANNNNNNNNNTGG 1 cut(s) 2105
XmiI GTMKAC 1 cut(s) 1088
XmnI GAANNNNTTC 2 cut(s) 1761, 1823
XspI CTAG 6 cut(s) 941, 1116, 1149, 1245, 1701, 2512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.