pycom15g27280

Alcohol dehydrogenase GroES-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005946
Physical Location & Seq
Forward (+)
22598343 .. 22599239
897 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
ATGGTCGCCAATGAGCGCTACATTGTTCGTTTCCCCGAGAACCTGCCACTTGATGCTGGTGCTCCTCTACTATGTGCTGGGATCACAGTGTACAGTCCCCTGAAATATTTTGGCTTATCTGAGCCTGGTAAGCATGTTGGCATTGTAAGCCTTGGCGGGCTTGGTCACACCGGTGTGAAATTCGCTAAGGCGTTCGGGTCAAAAGTGTCTGTGATTAGTACCTCCCCTGGCAAGAAAGATGAAGCTCTGAAGAAACTCGGGGTTGATTCATTCGTGGTTAGCTGCGACCAGGAGCAAATGAAGGCTGCCAGAGGCACATTGGATGGCATCATCGACACAGTCTCAGCTGCGCATCCCATTGTGCCCTTACTTGGTCTTTTGAAGCCCCATGGGAAGCTTATTCTGGTGGGTGTACCAGTAAAGCCACTAAACCTCCATGTGTTTCCTTTAATCACGGGAAGAAAGTCTGTGGCCGGTGGAATCGGAGGCATGAAGGAGACCCAGGAAATGATTGATTTTGCAGCAAAACACAACATAACGGCGGAGGTTGAAGTTATCTCAATGGATTACGTGAACACAGCAATGGAGCGTCTTGCTAAGAATGATGTAAGATACCGATTTGTCATTGATGTTGCAAACACACTGGCTGCTAGCGAAGCTTGA

Protein Analysis

221

Amino Acids

23.36

Weight (kDa)

8.79

Isoelectric Point (pI)

19.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 53 - 175 1e-18 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 351
Acc36I ACCTGC 1 cut(s) 51
AciI CCGC 2 cut(s) 156, 542
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 1 cut(s) 179
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 3 cut(s) 92, 220, 414
AfeI AGCGCT 1 cut(s) 17
AfiI CCNNNNNNNGG 1 cut(s) 371
AgeI ACCGGT 1 cut(s) 170
AgsI TTSAA 2 cut(s) 382, 551
AjnI CCWGG 4 cut(s) 124, 226, 288, 501
AleI CACNNNNGTG 2 cut(s) 171, 173
AluBI AGCT 5 cut(s) 245, 282, 347, 397, 659
AluI AGCT 5 cut(s) 245, 282, 347, 397, 659
Alw21I GWGCWC 1 cut(s) 64
Alw26I GTCTC 2 cut(s) 346, 491
AlwI GGATC 1 cut(s) 89
Ama87I CYCGRG 2 cut(s) 35, 257
Aor51HI AGCGCT 1 cut(s) 17
AoxI GGCC 1 cut(s) 471
ApeKI GCWGC 5 cut(s) 282, 305, 347, 521, 647
ApoI RAATTY 1 cut(s) 179
AsiGI ACCGGT 1 cut(s) 170
AspLEI GCGC 2 cut(s) 18, 352
AsuNHI GCTAGC 1 cut(s) 650
AvaI CYCGRG 2 cut(s) 35, 257
BaeGI GKGCMC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 64
BbvI GCAGC 5 cut(s) 269, 292, 334, 533, 634
BccI CCATC 1 cut(s) 317
BceAI ACGGC 1 cut(s) 555
BciT130I CCWGG 4 cut(s) 126, 228, 290, 503
BcoDI GTCTC 2 cut(s) 346, 491
BfaI CTAG 1 cut(s) 651
BfoI RGCGCY 1 cut(s) 19
BfuAI ACCTGC 1 cut(s) 51
BisI GCNGC 5 cut(s) 283, 306, 348, 522, 648
BlsI GCNGC 5 cut(s) 284, 307, 349, 523, 649
Bme1390I CCNGG 4 cut(s) 126, 228, 290, 503
BmeT110I CYCGRG 2 cut(s) 35, 257
BmrFI CCNGG 4 cut(s) 126, 228, 290, 503
BmsI GCATC 3 cut(s) 43, 336, 361
BmtI GCTAGC 1 cut(s) 654
Bpu10I CCTNAGC 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 571
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 4 cut(s) 151, 226, 388, 501
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse118I RCCGGY 2 cut(s) 170, 473
Bse1I ACTGG 2 cut(s) 416, 648
Bse3DI GCAATG 1 cut(s) 588
BseBI CCWGG 4 cut(s) 126, 228, 290, 503
BseDI CCNNGG 4 cut(s) 151, 226, 388, 501
BseGI GGATG 2 cut(s) 328, 352
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 1 cut(s) 588
BseMII CTCAG 2 cut(s) 111, 357
BseNI ACTGG 2 cut(s) 416, 648
BseRI GAGGAG 1 cut(s) 54
BseSI GKGCMC 1 cut(s) 366
BseXI GCAGC 5 cut(s) 269, 292, 334, 533, 634
BseYI CCCAGC 1 cut(s) 77
BshFI GGCC 1 cut(s) 473
BshTI ACCGGT 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 64
BsiHKCI CYCGRG 2 cut(s) 35, 257
BsiSI CCGG 2 cut(s) 171, 474
BslFI GGGAC 1 cut(s) 81
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 2 cut(s) 346, 491
BsmFI GGGAC 1 cut(s) 81
BsnI GGCC 1 cut(s) 473
Bso31I GGTCTC 1 cut(s) 491
BsoBI CYCGRG 2 cut(s) 35, 257
Bsp1286I GDGCHC 2 cut(s) 64, 366
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 1 cut(s) 81
Bsp19I CCATGG 1 cut(s) 388
BspACI CCGC 2 cut(s) 156, 542
BspANI GGCC 1 cut(s) 473
BspCNI CTCAG 2 cut(s) 112, 356
BspMI ACCTGC 1 cut(s) 51
BspOI GCTAGC 1 cut(s) 654
BspPI GGATC 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 588
BsrFI RCCGGY 2 cut(s) 170, 473
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 2 cut(s) 416, 648
BssAI RCCGGY 2 cut(s) 170, 473
BssECI CCNNGG 4 cut(s) 151, 226, 388, 501
BssMI GATC 1 cut(s) 81
BssT1I CCWWGG 2 cut(s) 151, 388
Bst2UI CCWGG 4 cut(s) 126, 228, 290, 503
Bst4CI ACNGT 3 cut(s) 88, 95, 340
BstAUI TGTACA 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 571
BstC8I GCNNGC 2 cut(s) 158, 652
BstDEI CTNAG 4 cut(s) 120, 186, 343, 597
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 2 cut(s) 328, 352
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 2 cut(s) 18, 352
BstKTI GATC 1 cut(s) 84
BstMAI GTCTC 2 cut(s) 346, 491
BstMBI GATC 1 cut(s) 81
BstMWI GCNNNNNNNGC 3 cut(s) 130, 147, 656
BstNI CCWGG 4 cut(s) 126, 228, 290, 503
BstNSI RCATGY 1 cut(s) 137
BstSCI CCNGG 4 cut(s) 124, 226, 288, 501
BstSLI GKGCMC 1 cut(s) 366
BstV1I GCAGC 5 cut(s) 269, 292, 334, 533, 634
BsuRI GGCC 1 cut(s) 473
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 2 cut(s) 328, 352
BtsIMutI CAGTG 2 cut(s) 93, 641
BveI ACCTGC 1 cut(s) 51
Cac8I GCNNGC 2 cut(s) 158, 652
CfoI GCGC 2 cut(s) 18, 352
Cfr10I RCCGGY 2 cut(s) 170, 473
CseI GACGC 1 cut(s) 578
Csp6I GTAC 3 cut(s) 91, 219, 413
CspAI ACCGGT 1 cut(s) 170
CviAII CATG 4 cut(s) 134, 389, 437, 490
CviQI GTAC 3 cut(s) 91, 219, 413
DdeI CTNAG 4 cut(s) 120, 186, 343, 597
DpnI GATC 1 cut(s) 83
DpnII GATC 1 cut(s) 81
EaeI YGGCCR 1 cut(s) 471
EciI GGCGGA 1 cut(s) 557
Eco130I CCWWGG 2 cut(s) 151, 388
Eco31I GGTCTC 1 cut(s) 491
Eco47III AGCGCT 1 cut(s) 17
Eco57I CTGAAG 1 cut(s) 269
Eco88I CYCGRG 2 cut(s) 35, 257
EcoRII CCWGG 4 cut(s) 124, 226, 288, 501
EcoT14I CCWWGG 2 cut(s) 151, 388
ErhI CCWWGG 2 cut(s) 151, 388
FaeI CATG 4 cut(s) 137, 392, 440, 493
FaiI YATR 6 cut(s) 73, 135, 390, 438, 491, 536
FaqI GGGAC 1 cut(s) 81
FatI CATG 4 cut(s) 133, 388, 436, 489
FauI CCCGC 1 cut(s) 149
Fnu4HI GCNGC 5 cut(s) 283, 306, 348, 522, 648
FokI GGATG 2 cut(s) 335, 339
Fsp4HI GCNGC 5 cut(s) 283, 306, 348, 522, 648
FspBI CTAG 1 cut(s) 651
FspI TGCGCA 1 cut(s) 351
GlaI GCGC 2 cut(s) 17, 351
GluI GCNGC 5 cut(s) 283, 306, 348, 522, 648
GsaI CCCAGC 1 cut(s) 81
HaeII RGCGCY 1 cut(s) 19
HaeIII GGCC 1 cut(s) 473
HapII CCGG 2 cut(s) 171, 474
HgaI GACGC 1 cut(s) 578
HhaI GCGC 2 cut(s) 18, 352
Hin1II CATG 4 cut(s) 137, 392, 440, 493
Hin6I GCGC 2 cut(s) 16, 350
HinP1I GCGC 2 cut(s) 16, 350
HindIII AAGCTT 2 cut(s) 395, 657
HinfI GANTC 2 cut(s) 266, 480
HpaII CCGG 2 cut(s) 171, 474
Hpy166II GTNNAC 3 cut(s) 91, 413, 574
Hpy188I TCNGA 3 cut(s) 121, 249, 485
Hpy8I GTNNAC 3 cut(s) 91, 413, 574
HpyAV CCTTC 2 cut(s) 295, 487
HpyCH4III ACNGT 3 cut(s) 88, 95, 340
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 2 cut(s) 521, 635
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 147, 656
HpyF3I CTNAG 4 cut(s) 120, 186, 343, 597
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 4 cut(s) 137, 392, 440, 493
HspAI GCGC 2 cut(s) 16, 350
Kzo9I GATC 1 cut(s) 81
LmnI GCTCC 3 cut(s) 67, 292, 586
Lsp1109I GCAGC 5 cut(s) 269, 292, 334, 533, 634
LweI GCATC 3 cut(s) 43, 336, 361
MaeI CTAG 1 cut(s) 651
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 1 cut(s) 164
MalI GATC 1 cut(s) 83
MboI GATC 1 cut(s) 81
MboII GAAGA 2 cut(s) 262, 471
MhlI GDGCHC 2 cut(s) 64, 366
MluCI AATT 1 cut(s) 179
MnlI CCTC 6 cut(s) 75, 232, 305, 443, 479, 538
MseI TTAA 1 cut(s) 449
MslI CAYNNNNRTG 3 cut(s) 171, 173, 581
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 2 cut(s) 171, 474
MspR9I CCNGG 4 cut(s) 126, 228, 290, 503
MvaI CCWGG 4 cut(s) 126, 228, 290, 503
MwoI GCNNNNNNNGC 3 cut(s) 130, 147, 656
NcoI CCATGG 1 cut(s) 388
NdeII GATC 1 cut(s) 81
NheI GCTAGC 1 cut(s) 650
NlaIII CATG 4 cut(s) 137, 392, 440, 493
NmuCI GTSAC 1 cut(s) 164
NsbI TGCGCA 1 cut(s) 351
NspI RCATGY 1 cut(s) 137
OliI CACNNNNGTG 2 cut(s) 171, 173
PfeI GAWTC 2 cut(s) 266, 480
PflFI GACNNNGTC 1 cut(s) 338
PinAI ACCGGT 1 cut(s) 170
PkrI GCNGC 5 cut(s) 284, 307, 349, 523, 649
Ppu21I YACGTR 1 cut(s) 571
Psp6I CCWGG 4 cut(s) 124, 226, 288, 501
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 4 cut(s) 124, 226, 288, 501
PsyI GACNNNGTC 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 3 cut(s) 92, 220, 414
RsaNI GTAC 3 cut(s) 91, 219, 413
RseI CAYNNNNRTG 3 cut(s) 171, 173, 581
SaqAI TTAA 1 cut(s) 449
SatI GCNGC 5 cut(s) 283, 306, 348, 522, 648
Sau3AI GATC 1 cut(s) 81
ScrFI CCNGG 4 cut(s) 126, 228, 290, 503
SduI GDGCHC 2 cut(s) 64, 366
SfaNI GCATC 3 cut(s) 43, 336, 361
SgrAI CRCCGGYG 1 cut(s) 170
SmiMI CAYNNNNRTG 3 cut(s) 171, 173, 581
Sse9I AATT 1 cut(s) 179
SsiI CCGC 2 cut(s) 156, 542
SspI AATATT 1 cut(s) 107
SspMI CTAG 1 cut(s) 651
StyD4I CCNGG 4 cut(s) 124, 226, 288, 501
StyI CCWWGG 2 cut(s) 151, 388
TaaI ACNGT 3 cut(s) 88, 95, 340
TaiI ACGT 1 cut(s) 573
TaqI TCGA 1 cut(s) 333
TasI AATT 1 cut(s) 179
TatI WGTACW 1 cut(s) 90
TfiI GAWTC 2 cut(s) 266, 480
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TscAI CASTG 2 cut(s) 93, 648
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 5 cut(s) 282, 305, 347, 521, 647
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 4 cut(s) 255, 258, 314, 506
TspRI CASTG 2 cut(s) 93, 648
Tth111I GACNNNGTC 1 cut(s) 338
XapI RAATTY 1 cut(s) 179
XceI RCATGY 1 cut(s) 137
XcmI CCANNNNNNNNNTGG 1 cut(s) 316
XspI CTAG 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.