Rh2CG221400

Alcohol dehydrogenase GroES-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0005946
Physical Location & Seq
Forward (+)
22198124 .. 22199910
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG221400.1

Sequence Viewer

Length: 369 bp
ATGCAGGCTGCTAAGGGCACATTTGATGGCATCCTTGATACAGTCTCTGCTGTGCATGCCATTTCGCCCTTGATTGACCTTTTGAAGTCCGATGGGAAGCTTATTATGCTGGGAGCACCAGACAAGCCACTAGAACTAGACGTCTATCCTCTACTCTTGGGAAGGAAGTTGGTGGCTGGTAGTGCAATCGGTGGCATGAAGGAGACTCAAGAGATGATTGATTTCGCCAGAGAGCAAAACATAATTGCGGATATTGAGGTTATTCTGGTGGACTATTTGAACACGGCAATGGAGCGTCTTGCTAAGAACGATGTTAGATACCGCTTTGTGGTCGACGTCGGAAACACTCTGGCTGCTACCGAGCCCTAA

Protein Analysis

122

Amino Acids

13.24

Weight (kDa)

4.64

Isoelectric Point (pI)

16.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 5 - 76 1.4e-09 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 144, 339
AccI GTMKAC 1 cut(s) 333
AciI CCGC 2 cut(s) 248, 322
AcyI GRCGYC 2 cut(s) 141, 336
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 2 cut(s) 85, 280
AluBI AGCT 1 cut(s) 100
AluI AGCT 1 cut(s) 100
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 2 cut(s) 49, 197
AlwNI CAGNNNCTG 1 cut(s) 47
ApeKI GCWGC 2 cut(s) 8, 353
BaeGI GKGCMC 1 cut(s) 20
BanII GRGCYC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 1 cut(s) 340
BccI CCATC 2 cut(s) 20, 86
BceAI ACGGC 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 49, 197
BfaI CTAG 2 cut(s) 131, 137
BisI GCNGC 2 cut(s) 9, 354
BlsI GCNGC 2 cut(s) 10, 355
BmsI GCATC 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 192
BsaHI GRCGYC 2 cut(s) 141, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse3DI GCAATG 1 cut(s) 294
BseGI GGATG 1 cut(s) 30
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 1 cut(s) 294
BseSI GKGCMC 1 cut(s) 20
BseXI GCAGC 1 cut(s) 340
BseYI CCCAGC 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 118
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 2 cut(s) 49, 197
Bsp1286I GDGCHC 3 cut(s) 20, 118, 366
BspACI CCGC 2 cut(s) 248, 322
BsrDI GCAATG 1 cut(s) 294
BssNI GRCGYC 2 cut(s) 141, 336
Bst4CI ACNGT 1 cut(s) 43
BstACI GRCGYC 2 cut(s) 141, 336
BstC8I GCNNGC 2 cut(s) 6, 57
BstDEI CTNAG 2 cut(s) 12, 303
BstF5I GGATG 1 cut(s) 30
BstMAI GTCTC 2 cut(s) 49, 197
BstMWI GCNNNNNNNGC 3 cut(s) 56, 106, 182
BstNSI RCATGY 1 cut(s) 59
BstSLI GKGCMC 1 cut(s) 20
BstV1I GCAGC 1 cut(s) 340
BtsCI GGATG 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 6, 57
CaiI CAGNNNCTG 1 cut(s) 47
CseI GACGC 1 cut(s) 284
CviAII CATG 2 cut(s) 56, 196
CviJI RGCY 6 cut(s) 8, 100, 127, 176, 353, 364
CviKI_1 RGCY 6 cut(s) 8, 100, 127, 176, 353, 364
DdeI CTNAG 2 cut(s) 12, 303
Eco24I GRGCYC 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 366
FaeI CATG 2 cut(s) 59, 199
FaiI YATR 4 cut(s) 57, 107, 197, 242
FatI CATG 2 cut(s) 55, 195
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 2 cut(s) 9, 354
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 2 cut(s) 9, 354
FspBI CTAG 2 cut(s) 131, 137
GluI GCNGC 2 cut(s) 9, 354
GsaI CCCAGC 1 cut(s) 113
HgaI GACGC 1 cut(s) 284
Hin1I GRCGYC 2 cut(s) 141, 336
Hin1II CATG 2 cut(s) 59, 199
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 98
HinfI GANTC 1 cut(s) 205
Hpy166II GTNNAC 2 cut(s) 271, 334
Hpy188I TCNGA 2 cut(s) 91, 341
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 2 cut(s) 271, 334
Hpy99I CGWCG 2 cut(s) 338, 341
HpyAV CCTTC 2 cut(s) 156, 193
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 2 cut(s) 141, 336
HpyCH4V TGCA 3 cut(s) 4, 55, 185
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 106, 182
HpyF3I CTNAG 2 cut(s) 12, 303
HpySE526I ACGT 2 cut(s) 141, 336
Hsp92I GRCGYC 2 cut(s) 141, 336
Hsp92II CATG 2 cut(s) 59, 199
LmnI GCTCC 2 cut(s) 113, 292
LpnPI CCDG 6 cut(s) 95, 132, 162, 241, 251, 335
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 1 cut(s) 39
MaeI CTAG 2 cut(s) 131, 137
MaeII ACGT 2 cut(s) 141, 336
MhlI GDGCHC 3 cut(s) 20, 118, 366
MluCI AATT 1 cut(s) 243
MlyI GAGTC 1 cut(s) 199
MmeI TCCRAC 1 cut(s) 319
MnlI CCTC 2 cut(s) 159, 250
MslI CAYNNNNRTG 1 cut(s) 287
MwoI GCNNNNNNNGC 3 cut(s) 56, 106, 182
NlaIII CATG 2 cut(s) 59, 199
NspI RCATGY 1 cut(s) 59
PaeI GCATGC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 10, 355
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
PspFI CCCAGC 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 287
SalI GTCGAC 1 cut(s) 332
SatI GCNGC 2 cut(s) 9, 354
SchI GAGTC 1 cut(s) 199
SduI GDGCHC 3 cut(s) 20, 118, 366
SetI ASST 5 cut(s) 81, 102, 144, 261, 339
SfaNI GCATC 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
SphI GCATGC 1 cut(s) 59
Sse9I AATT 1 cut(s) 243
SsiI CCGC 2 cut(s) 248, 322
SspMI CTAG 2 cut(s) 131, 137
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 2 cut(s) 144, 339
TaqI TCGA 1 cut(s) 333
TasI AATT 1 cut(s) 243
TseI GCWGC 2 cut(s) 8, 353
TspDTI ATGAA 1 cut(s) 212
XceI RCATGY 1 cut(s) 59
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 2 cut(s) 131, 137
ZraI GACGTC 2 cut(s) 142, 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.