pycom15g32090

PHD-zinc-finger like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004120
Physical Location & Seq
Forward (+)
30963648 .. 30973027
9380 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g32090.18

Sequence Viewer

Length: 4407 bp
ATGACTGGAGGCCGATGTCACGAGAAGAAGATGATGGGTAGGGGTGCGGACGGAGGTTGCGGCACCGAGGAGAGGCCTTGCCCAATTCCTAGGGTTCCGCCCAAAATTCCGGCGACTCAGCCGGAAATTCTCGAGAAGTCGTCGAGCTCTTTGAGAATTGATTTTTTCTCACAGGCTAAGAAGGCGTTGTGTGAGCGCTCGCCTTTTGATGTTGCCGAGGACGGCTCAGCTTCGAGTGTGCCCACCACCTTGCCTAGGGGCTTGGCTAGCTTTCTGTCGAGGCAGTCGGACAGTCGGAAGCGGCATAAGAAGTCGCATGCGGGTGCGGAGAAGAAGAGTTCTCGGCAGAGCGAGAGGTCCCGGTGTAACAACATTTGGTTGGAGACTGAGGATTACTTTAGACCCTTGACATTGCCTGATATCGACGCTTTATTTCAGGTATCCGAGCTTAGTAATTTAGCTTATCGCAAGTGTTTTTCGATTCCGGTTGTTGGAAATGTTCCAAGGCGGAATGCAAATGAGAACGTTAATGCAAATGGAGTTGCTGTCAATGAGGACGATGCAAGTGGTGGCAAGTTAAATAGTGTTGTTGTCAAGGATGAGAGTATAAATGGGGGGAATGCAAATGATTCCCATGTTAAGTATGAGAATGCAAACGATGGGGATGCTAATGATGGTGTCGTCAAGGCCGAGTGTGCGAATGATGGGAACGCCAAATCAGTTTCAGTTGAAAACGTGAATGAGAATGGTGGAAATGAAAATGGGGTTGTTGAAGATGAGGCCAAGACAGAGAGGGATGAGCATTCCATGGAAATTGATTGTGTGAGAGCTTCTGGGTTGCCTGTGGGGGAAAAGGGTTTTAATTCATATTCTCCCATTGGTGTTGAGTGGCTTTTAGGATACAGGAATAAGATTTCTTTAACATCAGAGCGCCCGTCAAAGAAGCGGAAGGTTCTGGGTGCTGATGCAGGGTTGGAGAAAGTGTTAATAGCCTCTCCATGTGATGGGAGTTCATCTTTATGCCATTTCTGCTGCATGGGTGATGCAGGCAAAGAGTCAAACCGATTGATTGTCTGTAGTTCTTGTAAGGTTGGAGTTCATCAGAAGTGCTATGGAGTGCTAGAGGATGTAGATGCATCATGGTTATGCTCTTGGTGTAAGCAGAAGATGGATACTAGTGATTCAGTCAAGCCTTGTGTGCTTTGCTCAAAGAAGGGTGGCGCTTTAAAGCCGGTTCTTAAGAGTGTTGAAAATGGTGGATCTCTGGATTTTGCTCACTTGTTTTGTTGTCAGTGGATGCCTGAGGTTTATATAGAGGACATAGCAAAGATGGAGCCTATCATAAATGTTGGAGGAATAACTGAAACTCGCAGAAAATTAATATGTAACATATGCAAGGTGAAGTGGGGCGCTTGTGTTCGATGCAGTCATGGAACTTGCAGAACTTCGTTTCATCCATTATGTGCAAGGGAGGCAAGACAGAGAATGGAAATTTGGGGAAAATTTGGGTGCGATAATGTTGAGCTGAGGGCATTTTGTTCAAAGCACTCAGAAGTACCAGATAACAGCAACAGTCAATTGGTATCTCCGTCCATCTCAGCTGACAAGAACTCAAATGTCTCCAACCATCTTCCGATGGCATTGTCGGAAAACAAATTAAACAAGTTAAGGATTGGCCCTAGAAATGCTGATAAAACTGCAGTCCCTCCAGATATTGTTTCTGACAACTCAGGTGATAGTGAATCACAGGAGATAGTGTTTCCTGTTCCAAGTTTAAATTCTGGGCTGACATCAGAGGGTGGTGAAGCACAGCCGATGATTGATGCAGGGGCATTTGAGAGAAGCAAAGAGGATGTTAATGCATCTAATTCCCTTAAATTTTCTCTGATTTTGAAGAAGCTGATTGATTGTGGAAAAGTTAATGTGAAGGATGTGGCATTGGACATTGGCCTCTCACCTGATTCCTTATCTGCTTCACTTGCTGATCATACTGTGGCGACTGATGTGCAAAACAGAATAGTTAAATGGCTCAGAGACCATTCTAACTTGGATTTAAAGCAGAAAAATGGGAAAATGAAATTGAGATCAGCAATTTCATCCATGACCGAGTTTGGGGGTTCTGATGGTTCTGAAGCTGCTTCATTATCAGAGGCTGATATGGCAGATGTAGCTGTTAAGTCAGTACCCCCACGGAGAAGAACCAAAAGTAGTATTAGGATTTTGAAGGATAACAAAGTAATAAGCTCATCCGAGGGGACCTGTAATGACAATGGCACATTGGACAAGATTAAGGTTGATCAACCCATCATTGACGAACAAGAAAATTTAAGTAAAGCGTCTGTTCCTGATCCTGTTGAGAAGAATCTGACGGAGGCCGATGTAGTTCAAAATTCTTTGCTGACACATTCACCCAGATCAGAAGGTCTTTCATCTAAACCCTTGAATTGCAGCCCTTTGCAAATGGGTCAAGAGCAGTTGGCTACTATTCCTTTGCAGGGCACCTCAGTTGTTCCTGAATTGAATAAATCTGTGACTGAAGAAGTTCCTGGTTGTTATGTCCATCCATATATTGAAAATAAGTTGTTGGAGATGCAGACCGGGGTGTCCTTAGAAAATCCAATATACGGTTCAAGTGATGGAGTAATCTCACGTTTGGAGGCATCTTCTCATGCCAGTGTTTGCTGTAATCACCAAAATAAGCATCCAAAATGCTGTGATATGATATGTAAGTCTGATGAGATGAATCTAGAACAGTTGGTAAAGGCTCAAGAAATGGGAGTTCTAGAAATGTCTCTAGAGGATGAAGTGGAAGGTGAACTCATTTATTACCAGCATAGGTTGCTCAACAATGCAATTGCAAGAAAGCATTTTACTGATAATTTAGTATCTTATGTTGCTAAGAGTCTTCCATTGGAGATTGATGCAGCACGGAAGCAAAAATGGGATGCTGTACTTGTTAACCAGTATCTTTGTGAGCTTAGGGAGGCAAAGAAGCAGGGTAGGAAGGAGAGACGGCATAAAGAAGCACAGGCTGTGCTAGCAGCTGCAACCGCTGCTGCTGCAGCCTCTTCTAGGATTTCATCATTTAGGAAAGATGCCCTTGACGAATCTTCCCATCAGGAGAATGTGACGAAGTTGAATACCTCTAGTGGGAGGTCCAGTTTTTCCTCACAGATGATGCCGCGGCCGAAAGAGACATTTCCGAGAGTGGCTGTTCCCAGGATTTCATTGGAAAAACACTCTGGTTTTGTTCAATTAGTCACTGATTTTTCCAAAGAACATCCTAGATCTCTCTGTGACATCTGCAGACGTTCGGAAACGTTGTTAAACCCGATCTTAGTCTGTTCTAGTTGCAAGGTTGCCATTCACTTGGATTGCTATCGTAGAACTAGAGAATCCGCAGGTCCATGGTATTGTGAATTATGTGAAGAACTGTCATCATCTTGTGGCGCTGGTGCACCTGTCAATTTTTGGGAGAAAGATCATTTTGTTGCAGAATGTGGTTTATGTGGTGGAAAAACAGGTGCATTTAGGAAGTGTTCTGATGGTAAATGGGTTCATGCCTTCTGTGCCGAGTGGATCTTCGAGTCAACATTCCGAAGGGGGCAAGTATCTCCCATTGAGGGAATGTGTAACTATGGCAATTGTCAGTCAACATTTCATCCCTCATGTGCTAGAAGTTCTGGTTTTTACATGCATGTGAAAACTGTCGGTGGAAAGATCCAACACAAGGCATACTGTGAAAAGCATAGTGTTGAGCAGAGGACAAAGGCTGAAACACAAAAACATGGAACTGAGGAGTTAGAGAACCTCAGGAAAATCAGAGTTGAACTAGAGAGGGTACGCCTACTTTGTGAAAGAATCATCAAACGAGAAAAAGTAAAGAGGGAACTGCTCATTTGTTCACATGACCTTCTTGCTGTGAGGAGAGATCACGTTGCTCGGTCCGTGCTTGTTAATAGTCGTTTCTTGCCACCTAATGTTAGTTCTGAATCAGCTACAACATCTCTTAATGATGTGACTGTGGATAGTACAATTTCTGTGAAGCGGCAAAGAGTTCCTATAACTATTGACAACGACCCGAAAACAGATGATGACAGCTCCACATCGCAAAACCATTCTACCAAAAGCCTTCCGGAGAGGCCACAACTTTCTGAGAAACAGATTCCATGCAGAGCTTCAAGTGCTGCCACCTGTAATCTTTTGGAAGATGGAGGATGGAGGTCAAAATCTAGGAAGCATGCCGAGACATTTGAGAAAGAACTGGTTATGACATCGGATCAAGCATCGATGAAAAATATGAAATTACCGAAAGGATATGCATATGTTCCTGCTGATTGCATTCCCAACGAGAAGCAGATCAACCAGGATTCTTGTTCTGGTGAACAACCGCAAGGAGATGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000785 GO:0001067 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005886 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008289 GO:0008757 GO:0009653 GO:0009791 GO:0009889 GO:0009908 GO:0009909 GO:0009987 GO:0010093 GO:0010314 GO:0010468 GO:0010556 GO:0016020 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031326 GO:0032259 GO:0032501 GO:0032502 GO:0034968 GO:0035091 GO:0036211 GO:0042054 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043414 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048580 GO:0048608 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0051276 GO:0051568 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0097159 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901981 GO:1903506 GO:1905392 GO:1905393 GO:2000026 GO:2000112 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHD_2 zf-HC5HC2H_2

Protein Analysis

1469

Amino Acids

161.3

Weight (kDa)

7.04

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2602
Acc36I ACCTGC 1 cut(s) 3392
AccB1I GGYRCC 2 cut(s) 62, 2498
AccB7I CCANNNNNTGG 1 cut(s) 1004
AccII CGCG 1 cut(s) 3184
AccIII TCCGGA 1 cut(s) 4135
AclI AACGTT 2 cut(s) 525, 3320
AclWI GGATC 5 cut(s) 1267, 2342, 3587, 3715, 4287
AcoI YGGCCR 1 cut(s) 3185
AcsI RAATTY 8 cut(s) 105, 126, 1491, 1502, 1777, 1877, 2323, 2389
AcuI CTGAAG 2 cut(s) 2151, 2556
AfaI GTAC 5 cut(s) 1557, 2184, 2952, 3843, 4033
AfeI AGCGCT 1 cut(s) 197
AflII CTTAAG 1 cut(s) 1238
AhlI ACTAGT 1 cut(s) 1175
AjnI CCWGG 3 cut(s) 2545, 3218, 4365
AjuI GAANNNNNNNTTGG 6 cut(s) 1477, 1509, 1762, 1794, 2611, 2643
Alw21I GWGCWC 2 cut(s) 149, 3460
Alw26I GTCTC 7 cut(s) 377, 1624, 2028, 2796, 3004, 3188, 4241
Alw44I GTGCAC 1 cut(s) 3456
AlwI GGATC 5 cut(s) 1267, 2342, 3587, 3715, 4287
AlwNI CAGNNNCTG 1 cut(s) 2153
Ama87I CYCGRG 1 cut(s) 131
Aor13HI TCCGGA 1 cut(s) 4135
Aor51HI AGCGCT 1 cut(s) 197
AoxI GGCC 9 cut(s) 10, 74, 687, 780, 1675, 1948, 2373, 3185, 4142
ApaLI GTGCAC 1 cut(s) 3456
ApoI RAATTY 8 cut(s) 105, 126, 1491, 1502, 1777, 1877, 2323, 2389
AseI ATTAAT 1 cut(s) 1379
Asp700I GAANNNNTTC 1 cut(s) 2541
AspA2I CCTAGG 2 cut(s) 89, 254
AspLEI GCGC 5 cut(s) 198, 933, 1223, 1412, 3452
AspS9I GGNCC 6 cut(s) 357, 1676, 2256, 3156, 3404, 3945
AsuC2I CCSGG 2 cut(s) 361, 2599
AsuHPI GGTGA 9 cut(s) 1052, 1411, 1745, 1814, 1947, 2400, 2681, 2825, 4394
AsuNHI GCTAGC 2 cut(s) 266, 3037
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 5 cut(s) 357, 2256, 3156, 3404, 3945
AvrII CCTAGG 2 cut(s) 89, 254
AxyI CCTNAGG 2 cut(s) 1302, 3812
BaeGI GKGCMC 3 cut(s) 243, 2501, 3460
BanI GGYRCC 2 cut(s) 62, 2498
BanII GRGCYC 1 cut(s) 149
BauI CACGAG 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 2897
Bbv12I GWGCWC 2 cut(s) 149, 3460
BbvCI CCTCAGC 1 cut(s) 1526
BceAI ACGGC 2 cut(s) 238, 3029
BcgI CGANNNNNNTGC 4 cut(s) 647, 681, 1987, 2021
BciT130I CCWGG 3 cut(s) 2547, 3220, 4367
BciVI GTATCC 3 cut(s) 451, 893, 1165
BclI TGATCA 2 cut(s) 1984, 2296
BcnI CCSGG 2 cut(s) 361, 2599
BcoDI GTCTC 7 cut(s) 377, 1624, 2028, 2796, 3004, 3188, 4241
BcuI ACTAGT 1 cut(s) 1175
BfmI CTRYAG 4 cut(s) 1075, 1698, 3060, 3304
BfoI RGCGCY 5 cut(s) 199, 934, 1224, 1413, 3453
BfrI CTTAAG 1 cut(s) 1238
BfuAI ACCTGC 1 cut(s) 3392
BfuI GTATCC 3 cut(s) 451, 893, 1165
BglII AGATCT 1 cut(s) 3287
BlnI CCTAGG 2 cut(s) 89, 254
BlpI GCTNAGC 1 cut(s) 226
Bme1390I CCNGG 5 cut(s) 361, 2547, 2599, 3220, 4367
Bme18I GGWCC 5 cut(s) 357, 2256, 3156, 3404, 3945
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 6 cut(s) 357, 1676, 2256, 3156, 3404, 3945
BmiI GGNNCC 6 cut(s) 64, 96, 359, 1335, 2257, 2500
BmrFI CCNGG 5 cut(s) 361, 2547, 2599, 3220, 4367
BmtI GCTAGC 2 cut(s) 270, 3041
BpiI GAAGAC 1 cut(s) 2897
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpmI CTGGAG 2 cut(s) 27, 1692
Bpu10I CCTNAGC 2 cut(s) 1526, 2978
Bpu1102I GCTNAGC 1 cut(s) 226
BpuEI CTTGAG 1 cut(s) 2751
BpuMI CCSGG 2 cut(s) 361, 2599
Bsa29I ATCGAT 1 cut(s) 4289
BsaBI GATNNNNATC 1 cut(s) 3378
BsaI GGTCTC 1 cut(s) 2028
BsaWI WCCGGW 2 cut(s) 484, 4135
BsaXI ACNNNNNCTCC 8 cut(s) 531, 561, 968, 998, 1742, 1772, 3145, 3175
Bse118I RCCGGY 1 cut(s) 1231
Bse1I ACTGG 5 cut(s) 10, 2673, 2962, 3159, 4269
Bse21I CCTNAGG 2 cut(s) 1302, 3812
Bse3DI GCAATG 1 cut(s) 410
Bse8I GATNNNNATC 1 cut(s) 3378
BseAI TCCGGA 1 cut(s) 4135
BseBI CCWGG 3 cut(s) 2547, 3220, 4367
BseCI ATCGAT 1 cut(s) 4289
BseJI GATNNNNATC 1 cut(s) 3378
BseMI GCAATG 1 cut(s) 410
BseNI ACTGG 5 cut(s) 10, 2673, 2962, 3159, 4269
BseRI GAGGAG 3 cut(s) 83, 3812, 3940
BseSI GKGCMC 3 cut(s) 243, 2501, 3460
BseX3I CGGCCG 1 cut(s) 3185
Bsh1236I CGCG 1 cut(s) 3184
Bsh1285I CGRYCG 1 cut(s) 3188
BshFI GGCC 9 cut(s) 12, 76, 689, 782, 1677, 1950, 2375, 3187, 4144
BshNI GGYRCC 2 cut(s) 62, 2498
BshVI ATCGAT 1 cut(s) 4289
BsiEI CGRYCG 1 cut(s) 3188
BsiHKAI GWGCWC 2 cut(s) 149, 3460
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 7 cut(s) 110, 122, 361, 485, 1232, 2598, 4136
BslFI GGGAC 3 cut(s) 343, 1688, 2269
BsmAI GTCTC 7 cut(s) 377, 1624, 2028, 2796, 3004, 3188, 4241
BsmBI CGTCTC 1 cut(s) 3004
BsmFI GGGAC 3 cut(s) 343, 1688, 2269
BsmI GAATGC 5 cut(s) 517, 625, 655, 802, 4341
BsnI GGCC 9 cut(s) 12, 76, 689, 782, 1677, 1950, 2375, 3187, 4144
Bso31I GGTCTC 1 cut(s) 2028
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 4 cut(s) 149, 243, 2501, 3460
Bsp13I TCCGGA 1 cut(s) 4135
Bsp1720I GCTNAGC 1 cut(s) 226
Bsp19I CCATGG 2 cut(s) 807, 3407
BspANI GGCC 9 cut(s) 12, 76, 689, 782, 1677, 1950, 2375, 3187, 4144
BspDI ATCGAT 1 cut(s) 4289
BspEI TCCGGA 1 cut(s) 4135
BspFNI CGCG 1 cut(s) 3184
BspLI GGNNCC 6 cut(s) 64, 96, 359, 1335, 2257, 2500
BspMAI CTGCAG 3 cut(s) 1702, 3064, 3308
BspMI ACCTGC 1 cut(s) 3392
BspOI GCTAGC 2 cut(s) 270, 3041
BspPI GGATC 5 cut(s) 1267, 2342, 3587, 3715, 4287
BspT107I GGYRCC 2 cut(s) 62, 2498
BspTI CTTAAG 1 cut(s) 1238
BspTNI GGTCTC 1 cut(s) 2028
BsrDI GCAATG 1 cut(s) 410
BsrFI RCCGGY 1 cut(s) 1231
BsrI ACTGG 5 cut(s) 10, 2673, 2962, 3159, 4269
BssAI RCCGGY 1 cut(s) 1231
BssSI CACGAG 1 cut(s) 20
BssT1I CCWWGG 5 cut(s) 89, 254, 503, 807, 3407
Bst2BI CACGAG 1 cut(s) 20
Bst2UI CCWGG 3 cut(s) 2547, 3220, 4367
Bst4CI ACNGT 9 cut(s) 293, 1574, 1993, 2627, 2754, 3435, 3709, 3740, 4024
Bst6I CTCTTC 2 cut(s) 329, 3073
BstAFI CTTAAG 1 cut(s) 1238
BstAPI GCANNNNNTGC 2 cut(s) 2839, 3053
BstC8I GCNNGC 6 cut(s) 200, 268, 318, 1048, 3039, 4242
BstDSI CCRYGG 4 cut(s) 807, 2189, 3182, 3407
BstENI CCTNNNNNAGG 2 cut(s) 253, 3070
BstFNI CGCG 1 cut(s) 3184
BstH2I RGCGCY 5 cut(s) 199, 934, 1224, 1413, 3453
BstHHI GCGC 5 cut(s) 198, 933, 1223, 1412, 3452
BstMAI GTCTC 7 cut(s) 377, 1624, 2028, 2796, 3004, 3188, 4241
BstMCI CGRYCG 1 cut(s) 3188
BstNI CCWGG 3 cut(s) 2547, 3220, 4367
BstNSI RCATGY 4 cut(s) 320, 3697, 3701, 4244
BstSCI CCNGG 5 cut(s) 359, 2545, 2597, 3218, 4365
BstSFI CTRYAG 4 cut(s) 1075, 1698, 3060, 3304
BstSLI GKGCMC 3 cut(s) 243, 2501, 3460
BstUI CGCG 1 cut(s) 3184
BstV2I GAAGAC 1 cut(s) 2897
BstX2I RGATCY 4 cut(s) 1259, 3287, 3579, 3720
BstXI CCANNNNNNTGG 1 cut(s) 3370
BstYI RGATCY 4 cut(s) 1259, 3287, 3579, 3720
BstZI CGGCCG 1 cut(s) 3185
Bsu15I ATCGAT 1 cut(s) 4289
Bsu36I CCTNAGG 2 cut(s) 1302, 3812
BsuI GTATCC 3 cut(s) 451, 893, 1165
BsuRI GGCC 9 cut(s) 12, 76, 689, 782, 1677, 1950, 2375, 3187, 4144
BsuTUI ATCGAT 1 cut(s) 4289
BtgI CCRYGG 4 cut(s) 807, 2189, 3182, 3407
BtgZI GCGATG 1 cut(s) 4092
BtsIMutI CAGTG 3 cut(s) 1298, 2680, 3261
BveI ACCTGC 1 cut(s) 3392
Cac8I GCNNGC 6 cut(s) 200, 268, 318, 1048, 3039, 4242
CaiI CAGNNNCTG 1 cut(s) 2153
CfoI GCGC 5 cut(s) 198, 933, 1223, 1412, 3452
Cfr10I RCCGGY 1 cut(s) 1231
Cfr13I GGNCC 6 cut(s) 357, 1676, 2256, 3156, 3404, 3945
Cfr42I CCGCGG 1 cut(s) 3185
ClaI ATCGAT 1 cut(s) 4289
CpoI CGGWCCG 1 cut(s) 3945
CseI GACGC 2 cut(s) 434, 2325
Csp6I GTAC 5 cut(s) 1556, 2183, 2951, 3842, 4032
CspI CGGWCCG 1 cut(s) 3945
CviQI GTAC 5 cut(s) 1556, 2183, 2951, 3842, 4032
DraI TTTAAA 3 cut(s) 1227, 1776, 2055
DrdI GACNNNNNNGTC 1 cut(s) 2602
DseDI GACNNNNNNGTC 1 cut(s) 2602
EaeI YGGCCR 1 cut(s) 3185
EagI CGGCCG 1 cut(s) 3185
Eam1104I CTCTTC 2 cut(s) 329, 3073
EarI CTCTTC 2 cut(s) 329, 3073
EciI GGCGGA 2 cut(s) 87, 523
Ecl136II GAGCTC 1 cut(s) 147
EclXI CGGCCG 1 cut(s) 3185
Eco130I CCWWGG 5 cut(s) 89, 254, 503, 807, 3407
Eco147I AGGCCT 1 cut(s) 76
Eco24I GRGCYC 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 2028
Eco32I GATATC 1 cut(s) 421
Eco47I GGWCC 5 cut(s) 357, 2256, 3156, 3404, 3945
Eco47III AGCGCT 1 cut(s) 197
Eco52I CGGCCG 1 cut(s) 3185
Eco53kI GAGCTC 1 cut(s) 147
Eco57I CTGAAG 2 cut(s) 2151, 2556
Eco81I CCTNAGG 2 cut(s) 1302, 3812
Eco88I CYCGRG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 147
EcoNI CCTNNNNNAGG 2 cut(s) 253, 3070
EcoO109I RGGNCCY 2 cut(s) 357, 2256
EcoRII CCWGG 3 cut(s) 2545, 3218, 4365
EcoRV GATATC 1 cut(s) 421
EcoT14I CCWWGG 5 cut(s) 89, 254, 503, 807, 3407
EcoT22I ATGCAT 4 cut(s) 1138, 1864, 3699, 4324
EcoT38I GRGCYC 1 cut(s) 149
ErhI CCWWGG 5 cut(s) 89, 254, 503, 807, 3407
Esp3I CGTCTC 1 cut(s) 3004
FalI AAGNNNNNCTT 2 cut(s) 3084, 3116
FaqI GGGAC 3 cut(s) 343, 1688, 2269
FauI CCCGC 1 cut(s) 313
FauNDI CATATG 2 cut(s) 1391, 4324
FbaI TGATCA 2 cut(s) 1984, 2296
FriOI GRGCYC 1 cut(s) 149
GlaI GCGC 5 cut(s) 197, 932, 1222, 1411, 3451
GsuI CTGGAG 2 cut(s) 27, 1692
HaeII RGCGCY 5 cut(s) 199, 934, 1224, 1413, 3453
HaeIII GGCC 9 cut(s) 12, 76, 689, 782, 1677, 1950, 2375, 3187, 4144
HapII CCGG 7 cut(s) 110, 122, 361, 485, 1232, 2598, 4136
HgaI GACGC 2 cut(s) 434, 2325
HhaI GCGC 5 cut(s) 198, 933, 1223, 1412, 3452
Hin6I GCGC 5 cut(s) 196, 931, 1221, 1410, 3450
HinP1I GCGC 5 cut(s) 196, 931, 1221, 1410, 3450
HincII GTYRAC 3 cut(s) 2959, 3591, 3654
HindII GTYRAC 3 cut(s) 2959, 3591, 3654
HpaI GTTAAC 1 cut(s) 2959
HpaII CCGG 7 cut(s) 110, 122, 361, 485, 1232, 2598, 4136
HphI GGTGA 9 cut(s) 1052, 1411, 1745, 1814, 1947, 2400, 2681, 2825, 4394
Hpy166II GTNNAC 7 cut(s) 2816, 2959, 3458, 3591, 3654, 3905, 4385
Hpy8I GTNNAC 7 cut(s) 2816, 2959, 3458, 3591, 3654, 3905, 4385
Hpy99I CGWCG 2 cut(s) 145, 428
HpyCH4III ACNGT 9 cut(s) 293, 1574, 1993, 2627, 2754, 3435, 3709, 3740, 4024
HpyCH4IV ACGT 6 cut(s) 525, 735, 2650, 3310, 3320, 3936
HpySE526I ACGT 6 cut(s) 525, 735, 2650, 3310, 3320, 3936
HspAI GCGC 5 cut(s) 196, 931, 1221, 1410, 3450
Kpn2I TCCGGA 1 cut(s) 4135
Ksp22I TGATCA 2 cut(s) 1984, 2296
KspAI GTTAAC 1 cut(s) 2959
KspI CCGCGG 1 cut(s) 3185
LmnI GCTCC 2 cut(s) 1333, 4106
MaeII ACGT 6 cut(s) 525, 735, 2650, 3310, 3320, 3936
MaeIII GTNAC 9 cut(s) 17, 365, 1385, 2530, 3127, 3259, 3296, 3632, 4018
MfeI CAATTG 3 cut(s) 1577, 2853, 3643
MflI RGATCY 4 cut(s) 1259, 3287, 3579, 3720
MhlI GDGCHC 4 cut(s) 149, 243, 2501, 3460
MlyI GAGTC 4 cut(s) 109, 1064, 2911, 3596
Mph1103I ATGCAT 4 cut(s) 1138, 1864, 3699, 4324
MroI TCCGGA 1 cut(s) 4135
MroXI GAANNNNTTC 1 cut(s) 2541
MslI CAYNNNNRTG 5 cut(s) 321, 1018, 1144, 2673, 3698
MspA1I CMGCKG 4 cut(s) 1601, 3044, 3053, 3184
MspCI CTTAAG 1 cut(s) 1238
MspI CCGG 7 cut(s) 110, 122, 361, 485, 1232, 2598, 4136
MspR9I CCNGG 5 cut(s) 361, 2547, 2599, 3220, 4367
MunI CAATTG 3 cut(s) 1577, 2853, 3643
Mva1269I GAATGC 5 cut(s) 517, 625, 655, 802, 4341
MvaI CCWGG 3 cut(s) 2547, 3220, 4367
MvnI CGCG 1 cut(s) 3184
NciI CCSGG 2 cut(s) 361, 2599
NcoI CCATGG 2 cut(s) 807, 3407
NdeI CATATG 2 cut(s) 1391, 4324
NheI GCTAGC 2 cut(s) 266, 3037
NlaIV GGNNCC 6 cut(s) 64, 96, 359, 1335, 2257, 2500
NmeAIII GCCGAG 5 cut(s) 241, 322, 715, 3598, 4270
NmuCI GTSAC 6 cut(s) 17, 2530, 3127, 3259, 3296, 4018
NsiI ATGCAT 4 cut(s) 1138, 1864, 3699, 4324
NspI RCATGY 4 cut(s) 320, 3697, 3701, 4244
PaeI GCATGC 2 cut(s) 320, 4244
PaeR7I CTCGAG 1 cut(s) 131
PceI AGGCCT 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 687
PctI GAATGC 5 cut(s) 517, 625, 655, 802, 4341
PdmI GAANNNNTTC 1 cut(s) 2541
PflMI CCANNNNNTGG 1 cut(s) 1004
PleI GAGTC 4 cut(s) 109, 1063, 2910, 3595
PpsI GAGTC 4 cut(s) 109, 1063, 2910, 3595
PpuMI RGGWCCY 2 cut(s) 357, 2256
PshBI ATTAAT 1 cut(s) 1379
Psp124BI GAGCTC 1 cut(s) 149
Psp1406I AACGTT 2 cut(s) 525, 3320
Psp5II RGGWCCY 2 cut(s) 357, 2256
Psp6I CCWGG 3 cut(s) 2545, 3218, 4365
PspGI CCWGG 3 cut(s) 2545, 3218, 4365
PspN4I GGNNCC 6 cut(s) 64, 96, 359, 1335, 2257, 2500
PspPI GGNCC 6 cut(s) 357, 1676, 2256, 3156, 3404, 3945
PspPPI RGGWCCY 2 cut(s) 357, 2256
PstI CTGCAG 3 cut(s) 1702, 3064, 3308
PstNI CAGNNNCTG 1 cut(s) 2153
PsuI RGATCY 4 cut(s) 1259, 3287, 3579, 3720
PvuII CAGCTG 2 cut(s) 1601, 3044
RsaI GTAC 5 cut(s) 1557, 2184, 2952, 3843, 4033
RsaNI GTAC 5 cut(s) 1556, 2183, 2951, 3842, 4032
RseI CAYNNNNRTG 5 cut(s) 321, 1018, 1144, 2673, 3698
Rsr2I CGGWCCG 1 cut(s) 3945
RsrII CGGWCCG 1 cut(s) 3945
SacI GAGCTC 1 cut(s) 149
SacII CCGCGG 1 cut(s) 3185
Sau96I GGNCC 6 cut(s) 357, 1676, 2256, 3156, 3404, 3945
SchI GAGTC 4 cut(s) 109, 1064, 2911, 3596
ScrFI CCNGG 5 cut(s) 361, 2547, 2599, 3220, 4367
SduI GDGCHC 4 cut(s) 149, 243, 2501, 3460
SfcI CTRYAG 4 cut(s) 1075, 1698, 3060, 3304
Sfr274I CTCGAG 1 cut(s) 131
Sfr303I CCGCGG 1 cut(s) 3185
SgrBI CCGCGG 1 cut(s) 3185
SinI GGWCC 5 cut(s) 357, 2256, 3156, 3404, 3945
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 5 cut(s) 321, 1018, 1144, 2673, 3698
SmlI CTYRAG 3 cut(s) 131, 1238, 2766
SmoI CTYRAG 3 cut(s) 131, 1238, 2766
SpeI ACTAGT 1 cut(s) 1175
SphI GCATGC 2 cut(s) 320, 4244
SseBI AGGCCT 1 cut(s) 76
SstI GAGCTC 1 cut(s) 149
StuI AGGCCT 1 cut(s) 76
StyD4I CCNGG 5 cut(s) 359, 2545, 2597, 3218, 4365
StyI CCWWGG 5 cut(s) 89, 254, 503, 807, 3407
TaaI ACNGT 9 cut(s) 293, 1574, 1993, 2627, 2754, 3435, 3709, 3740, 4024
TaiI ACGT 6 cut(s) 528, 738, 2653, 3313, 3323, 3939
TaqI TCGA 9 cut(s) 132, 143, 233, 278, 423, 479, 1420, 3585, 4289
TaqII GACCGA 2 cut(s) 2120, 3933
TatI WGTACW 2 cut(s) 2950, 4031
TauI GCSGC 5 cut(s) 63, 304, 3184, 3187, 4051
TscAI CASTG 3 cut(s) 1298, 2680, 3268
TseFI GTSAC 6 cut(s) 17, 2530, 3127, 3259, 3296, 4018
Tsp45I GTSAC 6 cut(s) 17, 2530, 3127, 3259, 3296, 4018
TspGWI ACGGA 6 cut(s) 66, 1578, 2206, 2384, 2944, 3937
TspRI CASTG 3 cut(s) 1298, 2680, 3268
Van91I CCANNNNNTGG 1 cut(s) 1004
Vha464I CTTAAG 1 cut(s) 1238
VneI GTGCAC 1 cut(s) 3456
VpaK11BI GGWCC 5 cut(s) 357, 2256, 3156, 3404, 3945
VspI ATTAAT 1 cut(s) 1379
XagI CCTNNNNNAGG 2 cut(s) 253, 3070
XapI RAATTY 8 cut(s) 105, 126, 1491, 1502, 1777, 1877, 2323, 2389
XbaI TCTAGA 3 cut(s) 2746, 2782, 2794
XceI RCATGY 4 cut(s) 320, 3697, 3701, 4244
XcmI CCANNNNNNNNNTGG 1 cut(s) 3226
XhoI CTCGAG 1 cut(s) 131
XmaJI CCTAGG 2 cut(s) 89, 254
XmnI GAANNNNTTC 1 cut(s) 2541
Zsp2I ATGCAT 4 cut(s) 1138, 1864, 3699, 4324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.