pycom15g36570

RIBOSOMAL protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004086
Physical Location & Seq
Reverse (-)
36557535 .. 36559651
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
ATGGTGACAATAATATCCTGCTCAGCATCTCCTCCATTAAGTGTCTGTGGGTCGCTCAGCCTCTCCTCCTCCGAGTTACTTCCAACCCGGTCGACTCAGCAGCTTTCATGGGTTTCTTCCTTTCCTCCGATTAACATCTCCATTGCAGACCCGCCCACTCCCACACTGAGTAGGAATTCTGTTGTTCGAGCTGCATGGACCCGGAGATCTCGAGGTGAAGCTGCAAAGAAGCCTAACAGGAAATCCTGGAAACAGAAGACTGATATGTATATGAGGCCATTCTTACTAAATGTTTTCTTTTCGAAAAAGTTTATCCACGCAAAAGTGATGCATCGGGGAACAAGCAAAGTGATATCAGTTGCTACCACCAACGCCAAGGATCTTCGGAGCAACTTACCTTCACTCACAGATCACAACGCGTGCAGAGTTATAGGGAGGCTTATTGCTGAGCGATCGAAGGAAGCCGATGTATATGCTTTGTCTTATGAGGCCCGAAAAGGTGAACGGATTGAAGGTAAGCTTGGGATTGTTCTTGATACCATTAAAGAGAACGGGATCATGTTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

190

Amino Acids

20.96

Weight (kDa)

10.25

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 90 - 188 6.8e-10 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 92
AccII CGCG 1 cut(s) 419
AciI CCGC 1 cut(s) 152
AclWI GGATC 2 cut(s) 387, 563
AcsI RAATTY 1 cut(s) 175
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 1 cut(s) 512
AjnI CCWGG 1 cut(s) 245
AjuI GAANNNNNNNTTGG 2 cut(s) 504, 536
AluBI AGCT 4 cut(s) 103, 191, 221, 520
AluI AGCT 4 cut(s) 103, 191, 221, 520
AlwI GGATC 2 cut(s) 387, 563
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 2 cut(s) 275, 489
ApeKI GCWGC 3 cut(s) 100, 191, 221
ApoI RAATTY 1 cut(s) 175
AspS9I GGNCC 2 cut(s) 198, 490
AsuC2I CCSGG 2 cut(s) 88, 202
AsuHPI GGTGA 3 cut(s) 16, 227, 512
AsuII TTCGAA 1 cut(s) 302
AvaI CYCGRG 1 cut(s) 210
AvaII GGWCC 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 263
BbvI GCAGC 3 cut(s) 112, 178, 208
BcgI CGANNNNNNTGC 2 cut(s) 455, 489
BciT130I CCWGG 1 cut(s) 247
BcnI CCSGG 2 cut(s) 88, 202
BglII AGATCT 1 cut(s) 206
BisI GCNGC 3 cut(s) 101, 192, 222
BlpI GCTNAGC 3 cut(s) 22, 56, 447
BlsI GCNGC 3 cut(s) 102, 193, 223
Bme1390I CCNGG 3 cut(s) 88, 202, 247
Bme18I GGWCC 1 cut(s) 198
BmeT110I CYCGRG 1 cut(s) 210
BmgT120I GGNCC 2 cut(s) 198, 490
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 3 cut(s) 88, 202, 247
BmsI GCATC 3 cut(s) 35, 318, 340
BpiI GAAGAC 1 cut(s) 263
Bpu1102I GCTNAGC 3 cut(s) 22, 56, 447
Bpu14I TTCGAA 1 cut(s) 302
BpuMI CCSGG 2 cut(s) 88, 202
BsaBI GATNNNNATC 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 375
Bse3DI GCAATG 1 cut(s) 141
Bse8I GATNNNNATC 1 cut(s) 134
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 1 cut(s) 375
BseJI GATNNNNATC 1 cut(s) 134
BseMI GCAATG 1 cut(s) 141
BseMII CTCAG 5 cut(s) 36, 70, 110, 158, 438
BseRI GAGGAG 3 cut(s) 21, 55, 58
BseXI GCAGC 3 cut(s) 112, 178, 208
BsgI GTGCAG 1 cut(s) 442
Bsh1236I CGCG 1 cut(s) 419
Bsh1285I CGRYCG 2 cut(s) 92, 455
BshFI GGCC 2 cut(s) 277, 491
BsiEI CGRYCG 2 cut(s) 92, 455
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 2 cut(s) 88, 202
BsnI GGCC 2 cut(s) 277, 491
BsoBI CYCGRG 1 cut(s) 210
Bsp119I TTCGAA 1 cut(s) 302
Bsp143I GATC 5 cut(s) 206, 379, 409, 452, 555
Bsp1720I GCTNAGC 3 cut(s) 22, 56, 447
BspACI CCGC 1 cut(s) 152
BspANI GGCC 2 cut(s) 277, 491
BspCNI CTCAG 5 cut(s) 35, 69, 109, 159, 439
BspFNI CGCG 1 cut(s) 419
BspLI GGNNCC 1 cut(s) 200
BspPI GGATC 2 cut(s) 387, 563
BspT104I TTCGAA 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 141
BssECI CCNNGG 1 cut(s) 375
BssMI GATC 5 cut(s) 206, 379, 409, 452, 555
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 247
BstBI TTCGAA 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 421
BstDEI CTNAG 5 cut(s) 22, 56, 96, 167, 447
BstFNI CGCG 1 cut(s) 419
BstKTI GATC 5 cut(s) 209, 382, 412, 455, 558
BstMBI GATC 5 cut(s) 206, 379, 409, 452, 555
BstMCI CGRYCG 2 cut(s) 92, 455
BstNI CCWGG 1 cut(s) 247
BstSCI CCNGG 3 cut(s) 86, 200, 245
BstUI CGCG 1 cut(s) 419
BstV1I GCAGC 3 cut(s) 112, 178, 208
BstV2I GAAGAC 1 cut(s) 263
BstX2I RGATCY 2 cut(s) 206, 379
BstYI RGATCY 2 cut(s) 206, 379
BsuRI GGCC 2 cut(s) 277, 491
BtsIMutI CAGTG 1 cut(s) 164
Cac8I GCNNGC 1 cut(s) 421
Cfr13I GGNCC 2 cut(s) 198, 490
CviAII CATG 3 cut(s) 108, 195, 559
DdeI CTNAG 5 cut(s) 22, 56, 96, 167, 447
DpnI GATC 5 cut(s) 208, 381, 411, 454, 557
DpnII GATC 5 cut(s) 206, 379, 409, 452, 555
Eco130I CCWWGG 1 cut(s) 375
Eco32I GATATC 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 198
Eco88I CYCGRG 1 cut(s) 210
EcoRI GAATTC 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 245
EcoRV GATATC 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 3 cut(s) 111, 198, 562
FalI AAGNNNNNCTT 2 cut(s) 504, 536
FatI CATG 3 cut(s) 107, 194, 558
FauI CCCGC 1 cut(s) 159
FblI GTMKAC 1 cut(s) 92
Fnu4HI GCNGC 3 cut(s) 101, 192, 222
Fsp4HI GCNGC 3 cut(s) 101, 192, 222
GluI GCNGC 3 cut(s) 101, 192, 222
HaeIII GGCC 2 cut(s) 277, 491
HapII CCGG 2 cut(s) 88, 202
Hin1II CATG 3 cut(s) 111, 198, 562
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 1 cut(s) 94
HpaII CCGG 2 cut(s) 88, 202
HphI GGTGA 3 cut(s) 16, 227, 512
Hpy166II GTNNAC 2 cut(s) 93, 503
Hpy188I TCNGA 3 cut(s) 73, 129, 387
Hpy188III TCNNGA 2 cut(s) 210, 533
Hpy8I GTNNAC 2 cut(s) 93, 503
HpyAV CCTTC 3 cut(s) 408, 451, 506
HpyCH4V TGCA 5 cut(s) 146, 194, 224, 331, 423
HpyF3I CTNAG 5 cut(s) 22, 56, 96, 167, 447
Hsp92II CATG 3 cut(s) 111, 198, 562
Kzo9I GATC 5 cut(s) 206, 379, 409, 452, 555
LmnI GCTCC 1 cut(s) 387
LpnPI CCDG 6 cut(s) 31, 101, 215, 223, 232, 259
Lsp1109I GCAGC 3 cut(s) 112, 178, 208
LweI GCATC 3 cut(s) 35, 318, 340
MaeIII GTNAC 2 cut(s) 4, 75
MalI GATC 5 cut(s) 208, 381, 411, 454, 557
MboI GATC 5 cut(s) 206, 379, 409, 452, 555
MboII GAAGA 3 cut(s) 108, 268, 374
MflI RGATCY 2 cut(s) 206, 379
MluCI AATT 1 cut(s) 175
MluI ACGCGT 1 cut(s) 417
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 9 cut(s) 42, 71, 76, 79, 135, 206, 267, 429, 481
Mph1103I ATGCAT 1 cut(s) 333
MseI TTAA 4 cut(s) 38, 132, 543, 568
MspI CCGG 2 cut(s) 88, 202
MspR9I CCNGG 3 cut(s) 88, 202, 247
MvaI CCWGG 1 cut(s) 247
MvnI CGCG 1 cut(s) 419
NciI CCSGG 2 cut(s) 88, 202
NdeII GATC 5 cut(s) 206, 379, 409, 452, 555
NlaIII CATG 3 cut(s) 111, 198, 562
NlaIV GGNNCC 1 cut(s) 200
NmuCI GTSAC 1 cut(s) 4
NsiI ATGCAT 1 cut(s) 333
NspV TTCGAA 1 cut(s) 302
PaeR7I CTCGAG 1 cut(s) 210
PfoI TCCNGGA 1 cut(s) 245
PkrI GCNGC 3 cut(s) 102, 193, 223
Ple19I CGATCG 1 cut(s) 455
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
Psp6I CCWGG 1 cut(s) 245
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 2 cut(s) 198, 490
PsuI RGATCY 2 cut(s) 206, 379
PvuI CGATCG 1 cut(s) 455
SalI GTCGAC 1 cut(s) 91
SaqAI TTAA 4 cut(s) 38, 132, 543, 568
SatI GCNGC 3 cut(s) 101, 192, 222
Sau3AI GATC 5 cut(s) 206, 379, 409, 452, 555
Sau96I GGNCC 2 cut(s) 198, 490
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 3 cut(s) 88, 202, 247
SetI ASST 8 cut(s) 105, 193, 217, 223, 400, 502, 517, 522
SfaNI GCATC 3 cut(s) 35, 318, 340
Sfr274I CTCGAG 1 cut(s) 210
SfuI TTCGAA 1 cut(s) 302
SinI GGWCC 1 cut(s) 198
SlaI CTCGAG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
Sse9I AATT 1 cut(s) 175
SsiI CCGC 1 cut(s) 152
StyD4I CCNGG 3 cut(s) 86, 200, 245
StyI CCWWGG 1 cut(s) 375
TaqI TCGA 5 cut(s) 92, 187, 211, 302, 455
TasI AATT 1 cut(s) 175
Tru1I TTAA 4 cut(s) 38, 132, 543, 568
Tru9I TTAA 4 cut(s) 38, 132, 543, 568
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 3 cut(s) 100, 191, 221
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 96
TspGWI ACGGA 1 cut(s) 520
TspRI CASTG 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 198
XapI RAATTY 1 cut(s) 175
XhoI CTCGAG 1 cut(s) 210
XmiI GTMKAC 1 cut(s) 92
Zsp2I ATGCAT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.