Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0004570

Physical Location & Seq

Genomic Coordinates Reverse (-)

8064063 .. 8067522

Gene Structure 3460 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2454 bp

ATGGCTCCGCCGCCACCGCTGGTTGCCCCCATGAAGGCAACATCCAATGGATCGTTTCAAGGCGAAAATCCCCTGGATTTTGCTATTCCCTTACTCATCGTCCAGATTGTCTTGGTCGTTGTTTTCACCAGAACACTTGCATTCCTTCTTAAACCTCTGCGGCAACCTCGAGTTATAGCCGAAGTCATTGGAGGGATATTGCTTGGGCCGTCGGCATTTGGGAGAAGCCAGAAGTTTCTACACACCGTTTTCCCGGCAAAAGCCATGACTGTACTGGACACAGTGGCAAACATCGGACTCCTCTTCTTCTTGTTCCTCGTCGGCCTCGAACTCGACATCCGCGCCATTCGCCGCACCGGTAAGAAATCCCTAGGCATCGCCGTCGCAGGAATCACCCTCCCTTTCGTTCTCGGCGTCTTCACCTCCTTCGCACTCCGCGCCACCGTCTCCAAAGGAGTCAGCCAAGGCCCCTTCCTCGTCTTCATCGGCGTCGCCCTCTCCATCACCGCCTTTCCCGTCCTCGCCCGCATCCTCGCCGAGCTCAAGCTCCTCACCACTGACGTCGGCCGCATCGCCATGTCGGCCGCCGCAGTGAACGACGTCGCAGCCTGGATCCTCCTCGCCCTGGCCATCGCCCTTACCGGAACCAACACCTCCCCGCTCGTCTCCGTGTGGGTCCTAGTATCCGGGGCCGGCTTCGTTGGCTTCTGCGTCATCGCCCTAAGACCCCTCCTAGCCATGATGGCGAGGCGGTCCCCCGATGGGGAGCCGGTGAGGGAGCTCTACATATGCATCACGTTGTCCCTCGTCCTGTCGGCGAGTTTCGTGACGGACGTAATTGGGATACACGCGCTGTTCGGCGCGTTCGTGGTTGGGATAATAGTCCCGAAGGAGGGGCCGTTCGCCGGGGTGCTGATAGAGAAGCTAGAGGATCTGGTGTCGGGGCTTTTCCTGCCGCTATATTTCGTGTCGAGTGGGCTGAAGACCAACGTGGCGACCATCAGCGGCGGGCAGTCGTGGGCGCTGCTGCTGCTGGTTATATTCACGGCGTCTTTCGGGAAGATTGTGGGGACGGTGACGGTGTCGATGGTGTGTAAGGTGCCGTTCAGGGAGGCGTTGGCACTTGGGTTTCTGATGAACACCAAGGGACTGGTGGAGCTCATTGTCCTCAATATTGGAAAGGATAGAAAGGTGCTAAACGACCAAACCTTCGCCATCTTCGTCCTAATGGCGTTATTCACCACCTTCATCACCACCCCACTAGTAATGGCAGTCTACAAGCCAGCACGCCGAGGCGCCCCCTACAAGCACCGCAGCGTCTTCCGCAAGAACCCTGACACGGAGCTCCGAATGCTGGCCCGCTGCCACAGCACCCGCAACATCCCCACCGTGATCAACCTCATCGAGTCCTCCCGCGGAACCCGCAAGCGCGGCCGCCTCACCGTCTACGCCATGCATCTCATGGAGCTGTCAGAGCGCTCCTCCGCCATCTCCATGGTCCACAAGGCCCGCAACAACGGCCTCCCCTTCTGGAACAAAAAAAACGACGAAAACAACAAGGACCAAATGGTCATTGCATTCGAGGCTTACGAAAAGCTCAGCTCCGTCAAGGTCCGCCCCATGACCGCCATCTCCTCCCTCGACAACATCCATGAGGACATCTGCGCCAGCGCCCACCAAAAGAATGCCGCCGTGATTTTACTCCCGTTTCACAAACACCAGAGGCTCGACGGCAACATGGAGTCTCTAGGAAACGCGTTTAGATCGGTCAACGAGCGTGTGCTTAAACACGCTCCCTGCTCCGTTGGGATTTTGGTGGACAGGGGTCTTGGCGGAACAGCCCAGGTTTCGGCCAGCGAGGTGTCGTATAATATTATGGTGGCGTTTTTCGGAGGGCGGGACGACAGGGAGGCGTTGGCGTACGGAATGAGGCTGGCAGAGCATCCCGGGATTGTGATGACATTGATTCGGTTTGTGGCCCCACAAGGAAAGACGTTGAATTTTGGCGCGAAATTGGTCGGGATAACTTCTGACAAGAACAAGGGGATTTTGAAAGAGGAAGACTGCGGCGGCGACCAGAAGGGGGACGACGAGTCGCTTTTAGCGGAGTACATGAATGTGATGAAGAATAGTGGGAATAAGGAGGGCGGCGGGGAGCCGTCGATGTTGTACGAGGAGAAGGTGGTGGACAGCAAGGCGGAGATTTGTGTCGTGTTGAAATCGATGGCTAAAAATGTCAATTTGTTTATTGTGGGGAGAATGCCTCCGACGGCGCCGTTGGTGGACAGTTCGAGTGACTGCGCGGAGTTGGGGCCGGTGGGAAGCTTCTTGGCGTCGTCAGACTTTTCGTCCACAGCGTCCGTTGTGGTGTTTCAACAGTATAATCCCACGTCGTCGCAGCCGCTTGTGGTGGAGGAAGCTGACTATGAGATGCCGGATACACCAATGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0031410 GO:0031982 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0097708 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

818

Amino Acids

88.12

Weight (kDa)

9.16

Isoelectric Point (pI)

34.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	41 - 422	3.6e-63	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	477 - 619	1.3e-55	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	623 - 662	1e-08	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17630 AT3G17630
fragaria_vesca	FvH4_4g21880
malus_domestica	MD13G1136000.v1.1 MD16G1131400.v1.1
prunus_persica	Prupe.1G209400_v2.0.a1
pyrus_communis	pycom16g11580
rosa_chinensis	RchiOBHm_Chr3g0483861
rosa_laevigata	RLG00000007178 RLG00000023257
rosa_multiflora	Rmu_co8183874.1_g000001 Rmu_co8462775.1_g000001 Rmu_sc0004940.1_g000012 Rmu_sc0005901.1_g000001 Rmu_sc0009599.1_g000012
rosa_roxburghii	Rroxscaffold_5G00369850 Rroxscaffold_6G00398090
rosa_rugosa	Rorug03G0208200.1 Rorug03G0208300 Rorug04G0222700 Rorug04G0222800
rosa_samantha	Rh3AG257000 Rh3BG293400 Rh3CG291700 Rh3DG286700 Rh4AG279900 Rh4AG280100 Rh4BG285300 Rh4BG285500 Rh4CG300900
rosa_wichuraiana	Rw3G023230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1568
AatII	GACGTC	2 cut(s)	564, 603
AccB1I	GGYRCC	3 cut(s)	1099, 1295, 2272
AccBSI	CCGCTC	1 cut(s)	661
AccI	GTMKAC	2 cut(s)	1275, 1446
AccII	CGCG	9 cut(s)	342, 438, 851, 863, 1416, 1431, 1757, 2009, 2303
AclWI	GGATC	4 cut(s)	58, 607, 620, 939
AcoI	YGGCCR	5 cut(s)	565, 582, 627, 1432, 1851
AcsI	RAATTY	1 cut(s)	1999
AcuI	CTGAAG	1 cut(s)	1001
AcyI	GRCGYC	8 cut(s)	414, 489, 561, 600, 1049, 1296, 2273, 2333
AfaI	GTAC	4 cut(s)	273, 1922, 2111, 2171
AfeI	AGCGCT	1 cut(s)	1478
AflIII	ACRYGT	1 cut(s)	1755
AgeI	ACCGGT	1 cut(s)	356
AgsI	TTSAA	5 cut(s)	59, 1999, 2053, 2218, 2375
AhdI	GACNNNNNGTC	2 cut(s)	2092, 2347
AhlI	ACTAGT	1 cut(s)	1261
AjiI	CACGTC	1 cut(s)	2391
AjnI	CCWGG	4 cut(s)	72, 608, 624, 1842
AloI	GAACNNNNNNTCC	4 cut(s)	321, 353, 884, 916
Alw21I	GWGCWC	4 cut(s)	543, 783, 1161, 1347
Alw26I	GTCTC	3 cut(s)	451, 670, 1749
AlwI	GGATC	4 cut(s)	58, 607, 620, 939
Ama87I	CYCGRG	2 cut(s)	168, 1946
Aor51HI	AGCGCT	1 cut(s)	1478
ApeKI	GCWGC	7 cut(s)	605, 1024, 1027, 1030, 1314, 1362, 2398
ApoI	RAATTY	1 cut(s)	1999
ArsI	GACNNNNNNTTYG	2 cut(s)	1193, 1225
AsiGI	ACCGGT	1 cut(s)	356
AspA2I	CCTAGG	1 cut(s)	370
AsuC2I	CCSGG	5 cut(s)	254, 688, 907, 1947, 1948
AvaI	CYCGRG	2 cut(s)	168, 1946
AvaII	GGWCC	5 cut(s)	676, 753, 1498, 1561, 1612
AvrII	CCTAGG	1 cut(s)	370
BalI	TGGCCA	1 cut(s)	629
BamHI	GGATCC	1 cut(s)	612
BanI	GGYRCC	3 cut(s)	1099, 1295, 2272
BanII	GRGCYC	4 cut(s)	543, 783, 1161, 1347
BbsI	GAAGAC	5 cut(s)	409, 472, 989, 1312, 2067
Bbv12I	GWGCWC	4 cut(s)	543, 783, 1161, 1347
BbvI	GCAGC	7 cut(s)	617, 1011, 1014, 1017, 1326, 1349, 2410
BcgI	CGANNNNNNTGC	2 cut(s)	364, 398
BciT130I	CCWGG	4 cut(s)	74, 610, 626, 1844
BciVI	GTATCC	3 cut(s)	694, 837, 2431
BclI	TGATCA	1 cut(s)	1392
BcnI	CCSGG	5 cut(s)	254, 688, 907, 1947, 1948
BcoDI	GTCTC	3 cut(s)	451, 670, 1749
BcuI	ACTAGT	1 cut(s)	1261
BfaI	CTAG	6 cut(s)	371, 680, 734, 926, 1262, 1748
BfoI	RGCGCY	5 cut(s)	1025, 1299, 1480, 1674, 2276
BfuI	GTATCC	3 cut(s)	694, 837, 2431
BglI	GCCNNNNNGGC	2 cut(s)	581, 743
BlnI	CCTAGG	1 cut(s)	370
BlpI	GCTNAGC	1 cut(s)	1598
Bme1390I	CCNGG	9 cut(s)	74, 254, 610, 626, 688, 907, 1844, 1947, 1948
Bme18I	GGWCC	5 cut(s)	676, 753, 1498, 1561, 1612
BmeRI	GACNNNNNGTC	2 cut(s)	2092, 2347
BmeT110I	CYCGRG	2 cut(s)	168, 1946
BmgBI	CACGTC	1 cut(s)	2391
BmrFI	CCNGG	9 cut(s)	74, 254, 610, 626, 688, 907, 1844, 1947, 1948
BmsI	GCATC	7 cut(s)	384, 537, 579, 801, 1465, 1951, 2421
BoxI	GACNNNNGTC	1 cut(s)	1824
BpiI	GAAGAC	5 cut(s)	409, 472, 989, 1312, 2067
BplI	GAGNNNNNCTC	4 cut(s)	1466, 1498, 2248, 2280
Bpu1102I	GCTNAGC	1 cut(s)	1598
BpuEI	CTTGAG	1 cut(s)	527
BpuMI	CCSGG	5 cut(s)	254, 688, 907, 1947, 1948
Bsa29I	ATCGAT	1 cut(s)	2222
BsaHI	GRCGYC	8 cut(s)	414, 489, 561, 600, 1049, 1296, 2273, 2333
BsaWI	WCCGGW	2 cut(s)	356, 641
BsaXI	ACNNNNNCTCC	2 cut(s)	884, 914
Bse118I	RCCGGY	4 cut(s)	356, 692, 769, 2314
Bse1I	ACTGG	2 cut(s)	279, 1155
Bse3DI	GCAATG	1 cut(s)	1572
BseBI	CCWGG	4 cut(s)	74, 610, 626, 1844
BseCI	ATCGAT	1 cut(s)	2222
BseGI	GGATG	6 cut(s)	41, 336, 528, 1380, 1647, 1942
BseMI	GCAATG	1 cut(s)	1572
BseMII	CTCAG	1 cut(s)	1612
BseNI	ACTGG	2 cut(s)	279, 1155
BseRI	GAGGAG	6 cut(s)	290, 539, 608, 1471, 1624, 2189
BseX3I	CGGCCG	3 cut(s)	565, 582, 1432
BseXI	GCAGC	7 cut(s)	617, 1011, 1014, 1017, 1326, 1349, 2410
Bsh1236I	CGCG	9 cut(s)	342, 438, 851, 863, 1416, 1431, 1757, 2009, 2303
Bsh1285I	CGRYCG	3 cut(s)	568, 585, 1435
BshNI	GGYRCC	3 cut(s)	1099, 1295, 2272
BshTI	ACCGGT	1 cut(s)	356
BshVI	ATCGAT	1 cut(s)	2222
BsiEI	CGRYCG	3 cut(s)	568, 585, 1435
BsiHKAI	GWGCWC	4 cut(s)	543, 783, 1161, 1347
BsiHKCI	CYCGRG	2 cut(s)	168, 1946
BsiWI	CGTACG	1 cut(s)	1920
BslFI	GGGAC	7 cut(s)	739, 787, 869, 1084, 1161, 1913, 2099
BsmAI	GTCTC	3 cut(s)	451, 670, 1749
BsmBI	CGTCTC	2 cut(s)	451, 670
BsmFI	GGGAC	7 cut(s)	739, 787, 869, 1084, 1161, 1913, 2099
BsmI	GAATGC	5 cut(s)	140, 1356, 1577, 1690, 2265
BsoBI	CYCGRG	2 cut(s)	168, 1946
Bsp1286I	GDGCHC	4 cut(s)	543, 783, 1161, 1347
Bsp143I	GATC	5 cut(s)	50, 612, 931, 1392, 1763
Bsp1720I	GCTNAGC	1 cut(s)	1598
Bsp19I	CCATGG	1 cut(s)	1494
BspCNI	CTCAG	1 cut(s)	1611
BspDI	ATCGAT	1 cut(s)	2222
BspFNI	CGCG	9 cut(s)	342, 438, 851, 863, 1416, 1431, 1757, 2009, 2303
BspPI	GGATC	4 cut(s)	58, 607, 620, 939
BspT107I	GGYRCC	3 cut(s)	1099, 1295, 2272
BsrBI	CCGCTC	1 cut(s)	661
BsrDI	GCAATG	1 cut(s)	1572
BsrFI	RCCGGY	4 cut(s)	356, 692, 769, 2314
BsrI	ACTGG	2 cut(s)	279, 1155
BssAI	RCCGGY	4 cut(s)	356, 692, 769, 2314
BssMI	GATC	5 cut(s)	50, 612, 931, 1392, 1763
BssNI	GRCGYC	8 cut(s)	414, 489, 561, 600, 1049, 1296, 2273, 2333
BssT1I	CCWWGG	4 cut(s)	370, 463, 1143, 1494
Bst2UI	CCWGG	4 cut(s)	74, 610, 626, 1844
Bst6I	CTCTTC	1 cut(s)	308
BstACI	GRCGYC	8 cut(s)	414, 489, 561, 600, 1049, 1296, 2273, 2333
BstDEI	CTNAG	2 cut(s)	722, 1598
BstDSI	CCRYGG	2 cut(s)	1414, 1494
BstF5I	GGATG	6 cut(s)	41, 336, 528, 1380, 1647, 1942
BstFNI	CGCG	9 cut(s)	342, 438, 851, 863, 1416, 1431, 1757, 2009, 2303
BstH2I	RGCGCY	5 cut(s)	1025, 1299, 1480, 1674, 2276
BstKTI	GATC	5 cut(s)	53, 615, 934, 1395, 1766
BstMAI	GTCTC	3 cut(s)	451, 670, 1749
BstMBI	GATC	5 cut(s)	50, 612, 931, 1392, 1763
BstMCI	CGRYCG	3 cut(s)	568, 585, 1435
BstNI	CCWGG	4 cut(s)	74, 610, 626, 1844
BstPAI	GACNNNNGTC	1 cut(s)	1824
BstSCI	CCNGG	9 cut(s)	72, 252, 608, 624, 686, 905, 1842, 1945, 1946
BstUI	CGCG	9 cut(s)	342, 438, 851, 863, 1416, 1431, 1757, 2009, 2303
BstV1I	GCAGC	7 cut(s)	617, 1011, 1014, 1017, 1326, 1349, 2410
BstV2I	GAAGAC	5 cut(s)	409, 472, 989, 1312, 2067
BstX2I	RGATCY	2 cut(s)	612, 931
BstXI	CCANNNNNNTGG	2 cut(s)	1150, 1495
BstYI	RGATCY	2 cut(s)	612, 931
BstZI	CGGCCG	3 cut(s)	565, 582, 1432
Bsu15I	ATCGAT	1 cut(s)	2222
BsuI	GTATCC	3 cut(s)	694, 837, 2431
BsuTUI	ATCGAT	1 cut(s)	2222
BtgI	CCRYGG	2 cut(s)	1414, 1494
BtgZI	GCGATG	4 cut(s)	361, 556, 616, 700
BtrI	CACGTC	1 cut(s)	2391
BtsCI	GGATG	6 cut(s)	41, 336, 528, 1380, 1647, 1942
BtsI	GCAGTG	1 cut(s)	597
BtsIMutI	CAGTG	3 cut(s)	288, 555, 597
CciNI	GCGGCCGC	1 cut(s)	1432
Cfr10I	RCCGGY	4 cut(s)	356, 692, 769, 2314
Cfr42I	CCGCGG	1 cut(s)	1417
Cfr9I	CCCGGG	1 cut(s)	1946
ClaI	ATCGAT	1 cut(s)	2222
CseI	GACGC	7 cut(s)	403, 478, 700, 1038, 1306, 2322, 2346
Csp6I	GTAC	4 cut(s)	272, 1921, 2110, 2170
CspAI	ACCGGT	1 cut(s)	356
CviQI	GTAC	4 cut(s)	272, 1921, 2110, 2170
DdeI	CTNAG	2 cut(s)	722, 1598
DinI	GGCGCC	2 cut(s)	1297, 2274
DpnI	GATC	5 cut(s)	52, 614, 933, 1394, 1765
DpnII	GATC	5 cut(s)	50, 612, 931, 1392, 1763
DrdI	GACNNNNNNGTC	1 cut(s)	1568
DriI	GACNNNNNGTC	2 cut(s)	2092, 2347
DseDI	GACNNNNNNGTC	1 cut(s)	1568
EaeI	YGGCCR	5 cut(s)	565, 582, 627, 1432, 1851
EagI	CGGCCG	3 cut(s)	565, 582, 1432
Eam1104I	CTCTTC	1 cut(s)	308
Eam1105I	GACNNNNNGTC	2 cut(s)	2092, 2347
EarI	CTCTTC	1 cut(s)	308
EciI	GGCGGA	4 cut(s)	1474, 1604, 1848, 2213
Ecl136II	GAGCTC	4 cut(s)	541, 781, 1159, 1345
EclXI	CGGCCG	3 cut(s)	565, 582, 1432
Eco130I	CCWWGG	4 cut(s)	370, 463, 1143, 1494
Eco24I	GRGCYC	4 cut(s)	543, 783, 1161, 1347
Eco47I	GGWCC	5 cut(s)	676, 753, 1498, 1561, 1612
Eco47III	AGCGCT	1 cut(s)	1478
Eco52I	CGGCCG	3 cut(s)	565, 582, 1432
Eco53kI	GAGCTC	4 cut(s)	541, 781, 1159, 1345
Eco57I	CTGAAG	1 cut(s)	1001
Eco88I	CYCGRG	2 cut(s)	168, 1946
EcoICRI	GAGCTC	4 cut(s)	541, 781, 1159, 1345
EcoO109I	RGGNCCY	2 cut(s)	467, 676
EcoRII	CCWGG	4 cut(s)	72, 608, 624, 1842
EcoT14I	CCWWGG	4 cut(s)	370, 463, 1143, 1494
EcoT22I	ATGCAT	2 cut(s)	794, 1458
EcoT38I	GRGCYC	4 cut(s)	543, 783, 1161, 1347
EgeI	GGCGCC	2 cut(s)	1297, 2274
EheI	GGCGCC	2 cut(s)	1297, 2274
ErhI	CCWWGG	4 cut(s)	370, 463, 1143, 1494
Esp3I	CGTCTC	2 cut(s)	451, 670
FaqI	GGGAC	7 cut(s)	739, 787, 869, 1084, 1161, 1913, 2099
FauNDI	CATATG	1 cut(s)	788
FbaI	TGATCA	1 cut(s)	1392
FblI	GTMKAC	2 cut(s)	1275, 1446
FokI	GGATG	6 cut(s)	28, 323, 515, 1367, 1634, 1929
FriOI	GRGCYC	4 cut(s)	543, 783, 1161, 1347
FspBI	CTAG	6 cut(s)	371, 680, 734, 926, 1262, 1748
HaeII	RGCGCY	5 cut(s)	1025, 1299, 1480, 1674, 2276
HgaI	GACGC	7 cut(s)	403, 478, 700, 1038, 1306, 2322, 2346
Hin1I	GRCGYC	8 cut(s)	414, 489, 561, 600, 1049, 1296, 2273, 2333
HincII	GTYRAC	1 cut(s)	1771
HindII	GTYRAC	1 cut(s)	1771
HindIII	AAGCTT	1 cut(s)	2323
HinfI	GANTC	7 cut(s)	297, 390, 456, 1406, 1742, 1966, 2093
Hpy166II	GTNNAC	9 cut(s)	595, 1276, 1447, 1501, 1771, 1819, 2188, 2284, 2352
Hpy188I	TCNGA	8 cut(s)	296, 1134, 1349, 1474, 1892, 2032, 2268, 2341
Hpy188III	TCNNGA	6 cut(s)	103, 826, 886, 1057, 1531, 2020
Hpy8I	GTNNAC	9 cut(s)	595, 1276, 1447, 1501, 1771, 1819, 2188, 2284, 2352
HpyCH4IV	ACGT	7 cut(s)	561, 600, 797, 834, 990, 1994, 2390
HpyCH4V	TGCA	4 cut(s)	140, 792, 1456, 1577
HpyF3I	CTNAG	2 cut(s)	722, 1598
HpySE526I	ACGT	7 cut(s)	561, 600, 797, 834, 990, 1994, 2390
Hsp92I	GRCGYC	8 cut(s)	414, 489, 561, 600, 1049, 1296, 2273, 2333
KasI	GGCGCC	2 cut(s)	1295, 2272
KroI	GCCGGC	1 cut(s)	692
KroNI	GCCGGC	1 cut(s)	694
Ksp22I	TGATCA	1 cut(s)	1392
KspI	CCGCGG	1 cut(s)	1417
Kzo9I	GATC	5 cut(s)	50, 612, 931, 1392, 1763
Lsp1109I	GCAGC	7 cut(s)	617, 1011, 1014, 1017, 1326, 1349, 2410
LweI	GCATC	7 cut(s)	384, 537, 579, 801, 1465, 1951, 2421
MaeI	CTAG	6 cut(s)	371, 680, 734, 926, 1262, 1748
MaeII	ACGT	7 cut(s)	561, 600, 797, 834, 990, 1994, 2390
MaeIII	GTNAC	3 cut(s)	826, 1075, 2294
MalI	GATC	5 cut(s)	52, 614, 933, 1394, 1765
MbiI	CCGCTC	1 cut(s)	661
MboI	GATC	5 cut(s)	50, 612, 931, 1392, 1763
MflI	RGATCY	2 cut(s)	612, 931
MhlI	GDGCHC	4 cut(s)	543, 783, 1161, 1347
MlsI	TGGCCA	1 cut(s)	629
MluCI	AATT	4 cut(s)	837, 1999, 2012, 2239
MluI	ACGCGT	1 cut(s)	1755
MluNI	TGGCCA	1 cut(s)	629
Mly113I	GGCGCC	2 cut(s)	1296, 2273
MlyI	GAGTC	5 cut(s)	291, 465, 1415, 1751, 2102
MmeI	TCCRAC	1 cut(s)	2291
Mox20I	TGGCCA	1 cut(s)	629
Mph1103I	ATGCAT	2 cut(s)	794, 1458
MroNI	GCCGGC	1 cut(s)	692
MscI	TGGCCA	1 cut(s)	629
MseI	TTAA	2 cut(s)	150, 1785
MslI	CAYNNNNRTG	3 cut(s)	575, 1493, 2117
Msp20I	TGGCCA	1 cut(s)	629
MspA1I	CMGCKG	4 cut(s)	19, 1005, 1362, 1416
MspR9I	CCNGG	9 cut(s)	74, 254, 610, 626, 688, 907, 1844, 1947, 1948
Mva1269I	GAATGC	5 cut(s)	140, 1356, 1577, 1690, 2265
MvaI	CCWGG	4 cut(s)	74, 610, 626, 1844
MvnI	CGCG	9 cut(s)	342, 438, 851, 863, 1416, 1431, 1757, 2009, 2303
NaeI	GCCGGC	1 cut(s)	694
NarI	GGCGCC	2 cut(s)	1296, 2273
NciI	CCSGG	5 cut(s)	254, 688, 907, 1947, 1948
NcoI	CCATGG	1 cut(s)	1494
NdeI	CATATG	1 cut(s)	788
NdeII	GATC	5 cut(s)	50, 612, 931, 1392, 1763
NgoMIV	GCCGGC	1 cut(s)	692
NmeAIII	GCCGAG	3 cut(s)	390, 562, 1316
NmuCI	GTSAC	3 cut(s)	826, 1075, 2294
NotI	GCGGCCGC	1 cut(s)	1432
NsiI	ATGCAT	2 cut(s)	794, 1458
PaeR7I	CTCGAG	1 cut(s)	168
PcsI	WCGNNNNNNNCGW	7 cut(s)	324, 411, 831, 864, 1218, 1587, 2354
PctI	GAATGC	5 cut(s)	140, 1356, 1577, 1690, 2265
PdiI	GCCGGC	1 cut(s)	694
PfeI	GAWTC	2 cut(s)	390, 1966
Pfl23II	CGTACG	1 cut(s)	1920
PflFI	GACNNNGTC	1 cut(s)	1081
PinAI	ACCGGT	1 cut(s)	356
PleI	GAGTC	5 cut(s)	291, 464, 1414, 1750, 2101
PluTI	GGCGCC	2 cut(s)	1299, 2276
PpsI	GAGTC	5 cut(s)	291, 464, 1414, 1750, 2101
PpuMI	RGGWCCY	1 cut(s)	676
PshAI	GACNNNNGTC	1 cut(s)	1824
Psp124BI	GAGCTC	4 cut(s)	543, 783, 1161, 1347
Psp5II	RGGWCCY	1 cut(s)	676
Psp6I	CCWGG	4 cut(s)	72, 608, 624, 1842
PspGI	CCWGG	4 cut(s)	72, 608, 624, 1842
PspLI	CGTACG	1 cut(s)	1920
PspPPI	RGGWCCY	1 cut(s)	676
PspXI	VCTCGAGB	1 cut(s)	168
PsuI	RGATCY	2 cut(s)	612, 931
PsyI	GACNNNGTC	1 cut(s)	1081
RsaI	GTAC	4 cut(s)	273, 1922, 2111, 2171
RsaNI	GTAC	4 cut(s)	272, 1921, 2110, 2170
RseI	CAYNNNNRTG	3 cut(s)	575, 1493, 2117
SacI	GAGCTC	4 cut(s)	543, 783, 1161, 1347
SacII	CCGCGG	1 cut(s)	1417
SaqAI	TTAA	2 cut(s)	150, 1785
Sau3AI	GATC	5 cut(s)	50, 612, 931, 1392, 1763
SchI	GAGTC	5 cut(s)	291, 465, 1415, 1751, 2102
ScrFI	CCNGG	9 cut(s)	74, 254, 610, 626, 688, 907, 1844, 1947, 1948
SduI	GDGCHC	4 cut(s)	543, 783, 1161, 1347
SfaNI	GCATC	7 cut(s)	384, 537, 579, 801, 1465, 1951, 2421
SfoI	GGCGCC	2 cut(s)	1297, 2274
Sfr274I	CTCGAG	1 cut(s)	168
Sfr303I	CCGCGG	1 cut(s)	1417
SgrBI	CCGCGG	1 cut(s)	1417
SinI	GGWCC	5 cut(s)	676, 753, 1498, 1561, 1612
SlaI	CTCGAG	1 cut(s)	168
SmaI	CCCGGG	1 cut(s)	1948
SmiMI	CAYNNNNRTG	3 cut(s)	575, 1493, 2117
SmlI	CTYRAG	2 cut(s)	168, 542
SmoI	CTYRAG	2 cut(s)	168, 542
SpeI	ACTAGT	1 cut(s)	1261
Sse9I	AATT	4 cut(s)	837, 1999, 2012, 2239
SspDI	GGCGCC	2 cut(s)	1295, 2272
SspI	AATATT	2 cut(s)	1174, 1873
SspMI	CTAG	6 cut(s)	371, 680, 734, 926, 1262, 1748
SstI	GAGCTC	4 cut(s)	543, 783, 1161, 1347
StyD4I	CCNGG	9 cut(s)	72, 252, 608, 624, 686, 905, 1842, 1945, 1946
StyI	CCWWGG	4 cut(s)	370, 463, 1143, 1494
TaiI	ACGT	7 cut(s)	564, 603, 800, 837, 993, 1997, 2393
TaqII	GACCGA	1 cut(s)	1756
TasI	AATT	4 cut(s)	837, 1999, 2012, 2239
TatI	WGTACW	2 cut(s)	271, 2109
TfiI	GAWTC	2 cut(s)	390, 1966
Tru1I	TTAA	2 cut(s)	150, 1785
Tru9I	TTAA	2 cut(s)	150, 1785
TscAI	CASTG	3 cut(s)	288, 562, 597
TseFI	GTSAC	3 cut(s)	826, 1075, 2294
TseI	GCWGC	7 cut(s)	605, 1024, 1027, 1030, 1314, 1362, 2398
Tsp45I	GTSAC	3 cut(s)	826, 1075, 2294
TspDTI	ATGAA	6 cut(s)	47, 472, 1151, 1237, 2129, 2138
TspGWI	ACGGA	7 cut(s)	658, 845, 1355, 1594, 1792, 1938, 2350
TspMI	CCCGGG	1 cut(s)	1946
TspRI	CASTG	3 cut(s)	288, 562, 597
Tth111I	GACNNNGTC	1 cut(s)	1081
VpaK11BI	GGWCC	5 cut(s)	676, 753, 1498, 1561, 1612
XapI	RAATTY	1 cut(s)	1999
XcmI	CCANNNNNNNNNTGG	3 cut(s)	271, 1150, 1459
XhoI	CTCGAG	1 cut(s)	168
XmaI	CCCGGG	1 cut(s)	1946
XmaJI	CCTAGG	1 cut(s)	370
XmiI	GTMKAC	2 cut(s)	1275, 1446
XspI	CTAG	6 cut(s)	371, 680, 734, 926, 1262, 1748
ZraI	GACGTC	2 cut(s)	562, 601
Zsp2I	ATGCAT	2 cut(s)	794, 1458

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.