pycom16g12140

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001572
Physical Location & Seq
Forward (+)
8478920 .. 8482058
3139 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1518 bp
ATGGAGGCTTTCGGAGGCGGATCTCGGAAGTTCTATAGTCCGACGGTGTCGGATTTGAAGGCGGTTGGGAAAAAGAGTTTGGAATGGGATTTAAATGATTGGCAATGGGATGGTGATCTTTTTACTGCTAGTCCTTTGAACGCTGCACCATCTGATTGTAGGAGTAGGCAGTTGTTTCCGCTTGGGCCGCCGGAAACTCCCTCCACTGCTGGTTTGTCCAACACTTCTTCTTCTGGTTCGGATGACATCTGTCCGGGGAATGAGAAAGGTAAGAGAGAGCTGGAGAAAAGAAGAAGGGATAGTATTGTAGAAAATGAAGAGTTAAACAATGAAGTTGGGTGTCTTAATCTCAAGCTTGGCGAGCAAGCTTACCCAATCATGGAAGGAGACGTACAAACTGGGAAGAAGACGAAGATAGTTGGGACTACTTTAAACCGGGCAGTTTGTCAGGTGGAGGACTGTAAGGCTGATCTTAGCGATGCCAAGGATTACCACCGACGACATAAGGTCTGTGATATGCATTCTAAGGCAACTAAAGCGCTGGTGGGAAATGTTTTGCAGCGGTTCTGTCAGCAGTGTAGCAGGTTTCATGTTCTTCAAGAGTTTGATGAAGGAAGGAGAAGTTGTCGTAGGCGTTTGGCTGGCCACAATAGGAGGAGAAGAAAAACAAATCCTGATGCTGTAGTTAATGAAGGCTCATGGAATGATGAAAGAGGAAGCAGTTATCTATTGATTAGTTTGCTGAGAATACTCTCCAACATCCACTCTAATAGTTCAGATCAAACAAAGGATCAGGATTTGCTTTCTCATCTTTTGAAAAGTCTAGCCAATCTTTCGGGTACAGTTGATGGAAGGAACATGTCTGCATTGCTTACGGCATCTCAAGGTTTACTAAATGGTGGGACATCAACTCAGACTGCACAAAAGGTCCTAGATACAGTTTCCAATGGCTGTGAACCAAGTAAGCCTTCTGTTTCAGCCTCTAAGATGAATGACTATGTTAATCGTGAGGACCCTTCAAGACCTATGCAACAGTGTTCTACAGTACCTGCATCAGATTTCAGGAGAATATCTTCAGTTGATGCCGATCATGGAGGTCTGCAAGTTGTTTCGGGTGTAAATGCCACCAAGCCATTTCCCTCAATAGACAGAGTTCCATCCACATCAGTTGCACCAGAAGCTACAACAGGGAGGATCCAGTTAAATGGCATTGATTTAAATAATACATATGACGATTCCCAGGACTATTTGGCCAGCCTAGGGAAATCTCAATCCCCTGTAAATTCAGGGACAGTGCCTCATGGTTTTCCCTTATGGATGCGGCAGGATTTGCAAAAGTCTAGCCCACCTCAGACAAGTGGGACTTCTTACTCAACTTCATCAAGTTCTAGTGGAGAGGTTCAGAGTCGCACGGATCGTATTGTTTTTAAACTCTTTGGAAAAGATCCAAATGATCTTCCAATTGTTCTATCGATCACAGATTCTCGACTGGTTGTCCCACAGTCCCACAGACATTGA

Protein Analysis

506

Amino Acids

55.26

Weight (kDa)

8.22

Isoelectric Point (pI)

57.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 149 - 222 4.4e-30 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 573, 1057
AciI CCGC 6 cut(s) 18, 62, 179, 188, 562, 1321
AclWI GGATC 6 cut(s) 28, 798, 1189, 1202, 1422, 1439
AcoI YGGCCR 2 cut(s) 643, 1251
AcsI RAATTY 1 cut(s) 1282
AcuI CTGAAG 1 cut(s) 1059
AfaI GTAC 3 cut(s) 393, 841, 1047
AfeI AGCGCT 1 cut(s) 540
AfiI CCNNNNNNNGG 2 cut(s) 379, 1260
AflIII ACRYGT 1 cut(s) 858
AgsI TTSAA 5 cut(s) 58, 139, 599, 817, 1020
AhdI GACNNNNNGTC 2 cut(s) 506, 1493
AjnI CCWGG 1 cut(s) 1239
AjuI GAANNNNNNNTTGG 4 cut(s) 62, 94, 952, 984
AluBI AGCT 4 cut(s) 280, 355, 368, 1181
AluI AGCT 4 cut(s) 280, 355, 368, 1181
Alw26I GTCTC 1 cut(s) 381
AlwI GGATC 6 cut(s) 28, 798, 1189, 1202, 1422, 1439
AlwNI CAGNNNCTG 1 cut(s) 1049
Aor51HI AGCGCT 1 cut(s) 540
AoxI GGCC 3 cut(s) 185, 643, 1251
ApeKI GCWGC 2 cut(s) 143, 559
ApoI RAATTY 1 cut(s) 1282
Asp700I GAANNNNTTC 1 cut(s) 1072
AspA2I CCTAGG 1 cut(s) 1258
AspLEI GCGC 1 cut(s) 541
AspS9I GGNCC 3 cut(s) 185, 928, 1012
AsuC2I CCSGG 2 cut(s) 255, 437
AsuHPI GGTGA 1 cut(s) 125
AvaII GGWCC 2 cut(s) 928, 1012
AvrII CCTAGG 1 cut(s) 1258
BalI TGGCCA 2 cut(s) 645, 1253
BamHI GGATCC 1 cut(s) 1194
BarI GAAGNNNNNNTAC 2 cut(s) 375, 407
BbsI GAAGAC 1 cut(s) 413
BbvI GCAGC 2 cut(s) 130, 571
BccI CCATC 4 cut(s) 104, 157, 842, 1165
BceAI ACGGC 1 cut(s) 891
BciT130I CCWGG 1 cut(s) 1241
BcnI CCSGG 2 cut(s) 255, 437
BcoDI GTCTC 1 cut(s) 381
BfaI CTAG 6 cut(s) 129, 824, 932, 1259, 1341, 1389
BfmI CTRYAG 3 cut(s) 34, 681, 1041
BfoI RGCGCY 1 cut(s) 542
BfuAI ACCTGC 2 cut(s) 573, 1057
BisI GCNGC 4 cut(s) 144, 188, 560, 1322
BlnI CCTAGG 1 cut(s) 1258
BlsI GCNGC 4 cut(s) 145, 189, 561, 1323
Bme1390I CCNGG 3 cut(s) 255, 437, 1241
Bme18I GGWCC 2 cut(s) 928, 1012
BmeRI GACNNNNNGTC 2 cut(s) 506, 1493
BmgT120I GGNCC 3 cut(s) 185, 928, 1012
BmiI GGNNCC 2 cut(s) 1014, 1196
BmrFI CCNGG 3 cut(s) 255, 437, 1241
BmrI ACTGGG 1 cut(s) 408
BmsI GCATC 6 cut(s) 469, 667, 887, 1061, 1072, 1308
BmuI ACTGGG 1 cut(s) 408
BoxI GACNNNNGTC 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 413
BpmI CTGGAG 1 cut(s) 302
BpuEI CTTGAG 2 cut(s) 335, 867
BpuMI CCSGG 2 cut(s) 255, 437
Bsa29I ATCGAT 1 cut(s) 1472
BsaBI GATNNNNATC 2 cut(s) 114, 1086
BsaJI CCNNGG 4 cut(s) 254, 483, 1239, 1258
BsaXI ACNNNNNCTCC 2 cut(s) 610, 640
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 1260
Bse1I ACTGG 3 cut(s) 403, 1198, 1494
Bse3DI GCAATG 2 cut(s) 110, 866
Bse8I GATNNNNATC 2 cut(s) 114, 1086
BseBI CCWGG 1 cut(s) 1241
BseCI ATCGAT 1 cut(s) 1472
BseDI CCNNGG 4 cut(s) 254, 483, 1239, 1258
BseGI GGATG 5 cut(s) 115, 247, 759, 1157, 1323
BseJI GATNNNNATC 2 cut(s) 114, 1086
BseLI CCNNNNNNNGG 2 cut(s) 379, 1260
BseMI GCAATG 2 cut(s) 110, 866
BseMII CTCAG 3 cut(s) 734, 926, 1364
BseNI ACTGG 3 cut(s) 403, 1198, 1494
BseRI GAGGAG 1 cut(s) 670
BseXI GCAGC 2 cut(s) 130, 571
BsgI GTGCAG 2 cut(s) 129, 903
BshFI GGCC 3 cut(s) 187, 645, 1253
BshVI ATCGAT 1 cut(s) 1472
BsiSI CCGG 3 cut(s) 191, 254, 436
BslFI GGGAC 6 cut(s) 436, 916, 1303, 1375, 1481, 1489
BslI CCNNNNNNNGG 2 cut(s) 379, 1260
BsmAI GTCTC 1 cut(s) 381
BsmBI CGTCTC 1 cut(s) 381
BsmFI GGGAC 6 cut(s) 436, 916, 1303, 1375, 1481, 1489
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 3 cut(s) 187, 645, 1253
BspACI CCGC 6 cut(s) 18, 62, 179, 188, 562, 1321
BspANI GGCC 3 cut(s) 187, 645, 1253
BspCNI CTCAG 3 cut(s) 735, 925, 1363
BspDI ATCGAT 1 cut(s) 1472
BspLI GGNNCC 2 cut(s) 1014, 1196
BspMI ACCTGC 2 cut(s) 573, 1057
BspPI GGATC 6 cut(s) 28, 798, 1189, 1202, 1422, 1439
BsrDI GCAATG 2 cut(s) 110, 866
BsrI ACTGG 3 cut(s) 403, 1198, 1494
BssECI CCNNGG 4 cut(s) 254, 483, 1239, 1258
BssT1I CCWWGG 2 cut(s) 483, 1258
Bst2UI CCWGG 1 cut(s) 1241
Bst4CI ACNGT 8 cut(s) 46, 461, 844, 940, 1035, 1045, 1294, 1503
Bst6I CTCTTC 1 cut(s) 312
BstAPI GCANNNNNTGC 1 cut(s) 1330
BstC8I GCNNGC 4 cut(s) 362, 366, 643, 1255
BstDEI CTNAG 6 cut(s) 473, 525, 743, 912, 984, 1350
BstF5I GGATG 5 cut(s) 115, 247, 759, 1157, 1323
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 1 cut(s) 541
BstMAI GTCTC 1 cut(s) 381
BstMWI GCNNNNNNNGC 5 cut(s) 187, 361, 536, 1178, 1330
BstNI CCWGG 1 cut(s) 1241
BstNSI RCATGY 1 cut(s) 862
BstPAI GACNNNNGTC 1 cut(s) 249
BstSCI CCNGG 3 cut(s) 253, 435, 1239
BstSFI CTRYAG 3 cut(s) 34, 681, 1041
BstV1I GCAGC 2 cut(s) 130, 571
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 3 cut(s) 20, 1194, 1444
BstXI CCANNNNNNTGG 1 cut(s) 1205
BstYI RGATCY 3 cut(s) 20, 1194, 1444
Bsu15I ATCGAT 1 cut(s) 1472
BsuRI GGCC 3 cut(s) 187, 645, 1253
BsuTUI ATCGAT 1 cut(s) 1472
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 5 cut(s) 115, 247, 759, 1157, 1323
BtsI GCAGTG 2 cut(s) 204, 581
BtsIMutI CAGTG 4 cut(s) 204, 581, 1040, 1299
BveI ACCTGC 2 cut(s) 573, 1057
Cac8I GCNNGC 4 cut(s) 362, 366, 643, 1255
CaiI CAGNNNCTG 1 cut(s) 1049
CfoI GCGC 1 cut(s) 541
Cfr13I GGNCC 3 cut(s) 185, 928, 1012
ClaI ATCGAT 1 cut(s) 1472
Csp6I GTAC 3 cut(s) 392, 840, 1046
CspCI CAANNNNNGTGG 3 cut(s) 1150, 1185, 1496
CviAII CATG 6 cut(s) 379, 590, 699, 859, 1091, 1301
CviQI GTAC 3 cut(s) 392, 840, 1046
DdeI CTNAG 6 cut(s) 473, 525, 743, 912, 984, 1350
DraI TTTAAA 4 cut(s) 93, 432, 1218, 1429
DriI GACNNNNNGTC 2 cut(s) 506, 1493
EaeI YGGCCR 2 cut(s) 643, 1251
Eam1104I CTCTTC 1 cut(s) 312
Eam1105I GACNNNNNGTC 2 cut(s) 506, 1493
EarI CTCTTC 1 cut(s) 312
EciI GGCGGA 1 cut(s) 33
Eco130I CCWWGG 2 cut(s) 483, 1258
Eco47I GGWCC 2 cut(s) 928, 1012
Eco47III AGCGCT 1 cut(s) 540
Eco57I CTGAAG 1 cut(s) 1059
EcoO109I RGGNCCY 2 cut(s) 928, 1012
EcoRII CCWGG 1 cut(s) 1239
EcoT14I CCWWGG 2 cut(s) 483, 1258
EcoT22I ATGCAT 1 cut(s) 522
ErhI CCWWGG 2 cut(s) 483, 1258
Esp3I CGTCTC 1 cut(s) 381
FaeI CATG 6 cut(s) 382, 593, 702, 862, 1094, 1304
FalI AAGNNNNNCTT 4 cut(s) 952, 984, 1348, 1380
FaqI GGGAC 6 cut(s) 436, 916, 1303, 1375, 1481, 1489
FatI CATG 6 cut(s) 378, 589, 698, 858, 1090, 1300
FauNDI CATATG 1 cut(s) 1228
Fnu4HI GCNGC 4 cut(s) 144, 188, 560, 1322
FokI GGATG 5 cut(s) 122, 254, 746, 1144, 1330
Fsp4HI GCNGC 4 cut(s) 144, 188, 560, 1322
FspBI CTAG 6 cut(s) 129, 824, 932, 1259, 1341, 1389
GlaI GCGC 1 cut(s) 540
GluI GCNGC 4 cut(s) 144, 188, 560, 1322
GsuI CTGGAG 1 cut(s) 302
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 3 cut(s) 187, 645, 1253
HapII CCGG 3 cut(s) 191, 254, 436
HhaI GCGC 1 cut(s) 541
Hin1II CATG 6 cut(s) 382, 593, 702, 862, 1094, 1304
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HindIII AAGCTT 2 cut(s) 353, 366
HinfI GANTC 3 cut(s) 1235, 1405, 1481
HpaII CCGG 3 cut(s) 191, 254, 436
HphI GGTGA 1 cut(s) 125
Hpy166II GTNNAC 2 cut(s) 890, 956
Hpy188III TCNNGA 7 cut(s) 599, 674, 794, 1007, 1020, 1063, 1485
Hpy8I GTNNAC 2 cut(s) 890, 956
Hpy99I CGWCG 2 cut(s) 46, 501
HpyAV CCTTC 9 cut(s) 52, 288, 377, 605, 609, 686, 846, 978, 1026
HpyCH4III ACNGT 8 cut(s) 46, 461, 844, 940, 1035, 1045, 1294, 1503
HpyCH4IV ACGT 1 cut(s) 390
HpyF10VI GCNNNNNNNGC 5 cut(s) 187, 361, 536, 1178, 1330
HpyF3I CTNAG 6 cut(s) 473, 525, 743, 912, 984, 1350
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 6 cut(s) 382, 593, 702, 862, 1094, 1304
HspAI GCGC 1 cut(s) 539
Lsp1109I GCAGC 2 cut(s) 130, 571
LweI GCATC 6 cut(s) 469, 667, 887, 1061, 1072, 1308
MaeI CTAG 6 cut(s) 129, 824, 932, 1259, 1341, 1389
MaeII ACGT 1 cut(s) 390
MfeI CAATTG 1 cut(s) 1461
MflI RGATCY 3 cut(s) 20, 1194, 1444
MlsI TGGCCA 2 cut(s) 645, 1253
MluCI AATT 2 cut(s) 1282, 1461
MluNI TGGCCA 2 cut(s) 645, 1253
MlyI GAGTC 1 cut(s) 1414
MmeI TCCRAC 4 cut(s) 30, 65, 243, 780
Mox20I TGGCCA 2 cut(s) 645, 1253
Mph1103I ATGCAT 1 cut(s) 522
MroXI GAANNNNTTC 1 cut(s) 1072
MscI TGGCCA 2 cut(s) 645, 1253
MseI TTAA 9 cut(s) 92, 323, 345, 431, 687, 1002, 1202, 1217, 1428
Msp20I TGGCCA 2 cut(s) 645, 1253
MspA1I CMGCKG 1 cut(s) 562
MspI CCGG 3 cut(s) 191, 254, 436
MspR9I CCNGG 3 cut(s) 255, 437, 1241
MunI CAATTG 1 cut(s) 1461
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 1 cut(s) 1241
MwoI GCNNNNNNNGC 5 cut(s) 187, 361, 536, 1178, 1330
NciI CCSGG 2 cut(s) 255, 437
NdeI CATATG 1 cut(s) 1228
NlaIII CATG 6 cut(s) 382, 593, 702, 862, 1094, 1304
NlaIV GGNNCC 2 cut(s) 1014, 1196
NsiI ATGCAT 1 cut(s) 522
NspI RCATGY 1 cut(s) 862
PciI ACATGT 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 1414
PctI GAATGC 1 cut(s) 520
PdmI GAANNNNTTC 1 cut(s) 1072
PfeI GAWTC 2 cut(s) 1235, 1481
PflFI GACNNNGTC 1 cut(s) 46
PkrI GCNGC 4 cut(s) 145, 189, 561, 1323
PleI GAGTC 1 cut(s) 1413
PpsI GAGTC 1 cut(s) 1413
PpuMI RGGWCCY 2 cut(s) 928, 1012
PscI ACATGT 1 cut(s) 858
PshAI GACNNNNGTC 1 cut(s) 249
Psp5II RGGWCCY 2 cut(s) 928, 1012
Psp6I CCWGG 1 cut(s) 1239
PspGI CCWGG 1 cut(s) 1239
PspN4I GGNNCC 2 cut(s) 1014, 1196
PspPI GGNCC 3 cut(s) 185, 928, 1012
PspPPI RGGWCCY 2 cut(s) 928, 1012
PstNI CAGNNNCTG 1 cut(s) 1049
PsuI RGATCY 3 cut(s) 20, 1194, 1444
PsyI GACNNNGTC 1 cut(s) 46
RsaI GTAC 3 cut(s) 393, 841, 1047
RsaNI GTAC 3 cut(s) 392, 840, 1046
SaqAI TTAA 9 cut(s) 92, 323, 345, 431, 687, 1002, 1202, 1217, 1428
SatI GCNGC 4 cut(s) 144, 188, 560, 1322
Sau96I GGNCC 3 cut(s) 185, 928, 1012
SchI GAGTC 1 cut(s) 1414
ScrFI CCNGG 3 cut(s) 255, 437, 1241
SfaNI GCATC 6 cut(s) 469, 667, 887, 1061, 1072, 1308
SfcI CTRYAG 3 cut(s) 34, 681, 1041
SinI GGWCC 2 cut(s) 928, 1012
SmiI ATTTAAAT 2 cut(s) 93, 1218
SmlI CTYRAG 2 cut(s) 350, 882
SmoI CTYRAG 2 cut(s) 350, 882
Sse9I AATT 2 cut(s) 1282, 1461
SsiI CCGC 6 cut(s) 18, 62, 179, 188, 562, 1321
SspMI CTAG 6 cut(s) 129, 824, 932, 1259, 1341, 1389
StyD4I CCNGG 3 cut(s) 253, 435, 1239
StyI CCWWGG 2 cut(s) 483, 1258
SwaI ATTTAAAT 2 cut(s) 93, 1218
TaaI ACNGT 8 cut(s) 46, 461, 844, 940, 1035, 1045, 1294, 1503
TaiI ACGT 1 cut(s) 393
TaqI TCGA 2 cut(s) 1472, 1486
TasI AATT 2 cut(s) 1282, 1461
TauI GCSGC 2 cut(s) 190, 1324
TfiI GAWTC 2 cut(s) 1235, 1481
Tru1I TTAA 9 cut(s) 92, 323, 345, 431, 687, 1002, 1202, 1217, 1428
Tru9I TTAA 9 cut(s) 92, 323, 345, 431, 687, 1002, 1202, 1217, 1428
TscAI CASTG 4 cut(s) 211, 581, 1040, 1299
TseI GCWGC 2 cut(s) 143, 559
TspDTI ATGAA 8 cut(s) 330, 345, 578, 624, 705, 723, 1004, 1368
TspGWI ACGGA 1 cut(s) 1427
TspRI CASTG 4 cut(s) 211, 581, 1040, 1299
Tth111I GACNNNGTC 1 cut(s) 46
VpaK11BI GGWCC 2 cut(s) 928, 1012
XapI RAATTY 1 cut(s) 1282
XceI RCATGY 1 cut(s) 862
XmaJI CCTAGG 1 cut(s) 1258
XmnI GAANNNNTTC 1 cut(s) 1072
XspI CTAG 6 cut(s) 129, 824, 932, 1259, 1341, 1389
Zsp2I ATGCAT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.