pycom16g12160

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001572
Physical Location & Seq
Forward (+)
8487109 .. 8489412
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1389 bp
ATGGTGCCGGATTTGACGGGAGTCGGGAAAAGGAGTATGGAATGGGATTTGAATGGTTGGAAATGGGATGGTGATCTTTTTACTGCTAGTCCATTGAATTCTACACCATCTGATGGTAGGAGTAGGCAGTTTTTTCCGGTCAGGCCAGAAACTCTGTTTGATGCTGGTTTGTCCAACAGTTCTTCTGGTTCGGATAATATCGGTCCGGGGAATGAGAAAGGTACAAGAGAATTGGAGAAACGGAGAAGGGATGTTTTTGTGGAAAACGGAGAGTTGAATGATGAAGCTGCATCTCTTAATCTTAAGCTCGGTGGGCAAACTTACCCCGTTATGGAAGAAGAAGTACAAACTGGGAAGAAAACAAGGTTAGTTGGGACTACTTCAAACCGCGCAGTTTGTCAGGTGGAGGACTGTAAGGCTGATCTTAGCAGTGCCAAGGATTACCACCGGCGGCATAAGGTCTGTGATATGCATTCAAAGGCAACTAAAGCACTGGTTGGAAATGTTATGCAGCGATTCTGTCAACAGTGTAGCAGGTTTCATGCTCTTCAAGAGTTTGATGAAGGGAAGAGAAGTTGCCGTAGGCGTTTGGCTGGCCATAATAGGAGGAGAAGAAAAACAAATCCTGATACTGCAGTTAATGGAGGCTCTTTAAATAATGAAAGTGGAAGCAGTTATCTATTGATTAGCTTGCTGAGAATACTCTCGAATATGCACTCTAGTAGTTCTGATCAAACAAAGGACCAGGATGTCGTATCTAATTTGTTGAGGAGCTTAGCCAATGTTGCTGGTATGGCTGATGGAAGAAACGTATCTAAATTGCTGCAGGGATCTCAAGGTTTATTTAACAGTGGGACATCTGTCCAGACTGCACGAAAGGTGCTAGATATGGATGATGGTGTTAATCCTGAGGACCCATTAAGGCCTAAAGGACATTGTTCAATATTACCAGCATCAAGAGACAGTTCGGAATCCAAATCAGTTACACCCGAACCTACAAGCAGAAGGTTCCAGTTGAATGACATTGATTTAAATAGTACATATGATGATTCACAGGACTTTGTAGAGAACCTAGGGAATACTCATGTTCCTGCAAGTCCAGGCACTGCATCTCCTGGTTTTCCTTCATGGATGCAGCGCGATTCTCACAAGTCAAGCCCACCTCAGACAAGCGGAAATTCAGACTTAACTTCTACTCAGTCACCATCAAGTTCTAGTGGAGAGGCTCAGGGCCAGTATGTAGAATGTATCTGCAATTTTACGTTTCTTAACCGCTCTTTTTCTGCCACATATCAGAGTCGCACAGACCGAATTGTTTTTAAACTATTTGGAAAAGACCCAAGTGAACTTCCGTTTGCTCTGCGATCACAGGTATTATTTTTCCCTTGA

Protein Analysis

463

Amino Acids

50.89

Weight (kDa)

8.03

Isoelectric Point (pI)

65.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 133 - 206 1.2e-30 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 749
Acc36I ACCTGC 1 cut(s) 525
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 113
AccBSI CCGCTC 1 cut(s) 1275
AccII CGCG 2 cut(s) 390, 1140
AciI CCGC 4 cut(s) 388, 451, 1173, 1273
AclWI GGATC 1 cut(s) 838
AcoI YGGCCR 1 cut(s) 595
AcsI RAATTY 2 cut(s) 97, 1177
AfaI GTAC 3 cut(s) 223, 345, 1039
AfiI CCNNNNNNNGG 2 cut(s) 113, 331
AflII CTTAAG 1 cut(s) 302
AgsI TTSAA 8 cut(s) 52, 97, 277, 384, 477, 551, 942, 1018
AjnI CCWGG 3 cut(s) 744, 1099, 1114
AluBI AGCT 4 cut(s) 287, 307, 690, 774
AluI AGCT 4 cut(s) 287, 307, 690, 774
Alw26I GTCTC 1 cut(s) 954
AlwI GGATC 1 cut(s) 838
AlwNI CAGNNNCTG 1 cut(s) 1106
AoxI GGCC 4 cut(s) 143, 595, 923, 1231
ApeKI GCWGC 4 cut(s) 287, 511, 823, 1135
ApoI RAATTY 2 cut(s) 97, 1177
AspA2I CCTAGG 1 cut(s) 1072
AspLEI GCGC 2 cut(s) 392, 1140
AspS9I GGNCC 4 cut(s) 203, 742, 913, 1231
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 2 cut(s) 83, 1194
AvaII GGWCC 3 cut(s) 203, 742, 913
AvrII CCTAGG 1 cut(s) 1072
AxyI CCTNAGG 1 cut(s) 909
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BalI TGGCCA 1 cut(s) 597
BanI GGYRCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 327, 359
BbvI GCAGC 4 cut(s) 274, 523, 810, 1147
BccI CCATC 6 cut(s) 62, 107, 115, 794, 890, 1213
BceAI ACGGC 1 cut(s) 564
BciT130I CCWGG 3 cut(s) 746, 1101, 1116
BclI TGATCA 1 cut(s) 730
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 954
BfaI CTAG 5 cut(s) 87, 720, 884, 1073, 1215
BfmI CTRYAG 2 cut(s) 633, 824
BfrI CTTAAG 1 cut(s) 302
BfuAI ACCTGC 1 cut(s) 525
BisI GCNGC 5 cut(s) 288, 452, 512, 824, 1136
BlnI CCTAGG 1 cut(s) 1072
BlpI GCTNAGC 1 cut(s) 775
BlsI GCNGC 5 cut(s) 289, 453, 513, 825, 1137
Bme1390I CCNGG 4 cut(s) 207, 746, 1101, 1116
Bme18I GGWCC 3 cut(s) 203, 742, 913
BmgT120I GGNCC 4 cut(s) 203, 742, 913, 1231
BmiI GGNNCC 3 cut(s) 6, 915, 1010
BmrFI CCNGG 4 cut(s) 207, 746, 1101, 1116
BmrI ACTGGG 1 cut(s) 360
BmsI GCATC 5 cut(s) 151, 299, 962, 1118, 1122
BmuI ACTGGG 1 cut(s) 360
BoxI GACNNNNGTC 2 cut(s) 20, 860
Bpu10I CCTNAGC 1 cut(s) 1227
Bpu1102I GCTNAGC 1 cut(s) 775
BpuEI CTTGAG 1 cut(s) 819
BpuMI CCSGG 1 cut(s) 207
BsaBI GATNNNNATC 1 cut(s) 72
BsaJI CCNNGG 3 cut(s) 206, 435, 1072
BsaWI WCCGGW 1 cut(s) 136
BsaXI ACNNNNNCTCC 2 cut(s) 1096, 1126
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 331
Bse118I RCCGGY 1 cut(s) 447
Bse1I ACTGG 4 cut(s) 355, 498, 1012, 1234
Bse21I CCTNAGG 1 cut(s) 909
Bse8I GATNNNNATC 1 cut(s) 72
BseBI CCWGG 3 cut(s) 746, 1101, 1116
BseDI CCNNGG 3 cut(s) 206, 435, 1072
BseGI GGATG 5 cut(s) 73, 256, 754, 898, 1137
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 2 cut(s) 113, 331
BseMII CTCAG 5 cut(s) 686, 900, 1178, 1211, 1241
BseNI ACTGG 4 cut(s) 355, 498, 1012, 1234
BseRI GAGGAG 2 cut(s) 622, 784
BseXI GCAGC 4 cut(s) 274, 523, 810, 1147
BsgI GTGCAG 1 cut(s) 855
Bsh1236I CGCG 2 cut(s) 390, 1140
BshFI GGCC 4 cut(s) 145, 597, 925, 1233
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 4 cut(s) 8, 137, 206, 448
BslFI GGGAC 2 cut(s) 388, 868
BslI CCNNNNNNNGG 2 cut(s) 113, 331
BsmAI GTCTC 1 cut(s) 954
BsmFI GGGAC 2 cut(s) 388, 868
BsmI GAATGC 1 cut(s) 472
BsnI GGCC 4 cut(s) 145, 597, 925, 1233
Bsp143I GATC 5 cut(s) 73, 421, 730, 830, 1364
Bsp1720I GCTNAGC 1 cut(s) 775
BspACI CCGC 4 cut(s) 388, 451, 1173, 1273
BspANI GGCC 4 cut(s) 145, 597, 925, 1233
BspCNI CTCAG 5 cut(s) 687, 901, 1177, 1210, 1240
BspFNI CGCG 2 cut(s) 390, 1140
BspLI GGNNCC 3 cut(s) 6, 915, 1010
BspMAI CTGCAG 2 cut(s) 637, 828
BspMI ACCTGC 1 cut(s) 525
BspPI GGATC 1 cut(s) 838
BspQI GCTCTTC 1 cut(s) 552
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 1275
BsrFI RCCGGY 1 cut(s) 447
BsrI ACTGG 4 cut(s) 355, 498, 1012, 1234
BssAI RCCGGY 1 cut(s) 447
BssECI CCNNGG 3 cut(s) 206, 435, 1072
BssMI GATC 5 cut(s) 73, 421, 730, 830, 1364
BssT1I CCWWGG 2 cut(s) 435, 1072
Bst2UI CCWGG 3 cut(s) 746, 1101, 1116
Bst4CI ACNGT 5 cut(s) 179, 413, 528, 851, 965
Bst6I CTCTTC 2 cut(s) 552, 563
BstAFI CTTAAG 1 cut(s) 302
BstC8I GCNNGC 2 cut(s) 595, 692
BstDEI CTNAG 7 cut(s) 425, 695, 775, 909, 1164, 1197, 1227
BstF5I GGATG 5 cut(s) 73, 256, 754, 898, 1137
BstFNI CGCG 2 cut(s) 390, 1140
BstHHI GCGC 2 cut(s) 392, 1140
BstKTI GATC 5 cut(s) 76, 424, 733, 833, 1367
BstMAI GTCTC 1 cut(s) 954
BstMBI GATC 5 cut(s) 73, 421, 730, 830, 1364
BstMWI GCNNNNNNNGC 4 cut(s) 313, 488, 785, 794
BstNI CCWGG 3 cut(s) 746, 1101, 1116
BstPAI GACNNNNGTC 2 cut(s) 20, 860
BstSCI CCNGG 4 cut(s) 205, 744, 1099, 1114
BstSFI CTRYAG 2 cut(s) 633, 824
BstUI CGCG 2 cut(s) 390, 1140
BstV1I GCAGC 4 cut(s) 274, 523, 810, 1147
BstX2I RGATCY 1 cut(s) 830
BstYI RGATCY 1 cut(s) 830
Bsu36I CCTNAGG 1 cut(s) 909
BsuRI GGCC 4 cut(s) 145, 597, 925, 1233
BtsCI GGATG 5 cut(s) 73, 256, 754, 898, 1137
BtsI GCAGTG 2 cut(s) 436, 1104
BtsIMutI CAGTG 5 cut(s) 436, 491, 533, 856, 1104
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 2 cut(s) 595, 692
CaiI CAGNNNCTG 1 cut(s) 1106
CfoI GCGC 2 cut(s) 392, 1140
Cfr10I RCCGGY 1 cut(s) 447
Cfr13I GGNCC 4 cut(s) 203, 742, 913, 1231
CpoI CGGWCCG 1 cut(s) 203
Csp6I GTAC 3 cut(s) 222, 344, 1038
CspI CGGWCCG 1 cut(s) 203
CviAII CATG 3 cut(s) 542, 1085, 1128
CviQI GTAC 3 cut(s) 222, 344, 1038
DdeI CTNAG 7 cut(s) 425, 695, 775, 909, 1164, 1197, 1227
DpnI GATC 5 cut(s) 75, 423, 732, 832, 1366
DpnII GATC 5 cut(s) 73, 421, 730, 830, 1364
DraI TTTAAA 3 cut(s) 654, 1032, 1321
DrdI GACNNNNNNGTC 1 cut(s) 749
DseDI GACNNNNNNGTC 1 cut(s) 749
EaeI YGGCCR 1 cut(s) 595
Eam1104I CTCTTC 2 cut(s) 552, 563
EarI CTCTTC 2 cut(s) 552, 563
Eco130I CCWWGG 2 cut(s) 435, 1072
Eco147I AGGCCT 1 cut(s) 925
Eco47I GGWCC 3 cut(s) 203, 742, 913
Eco81I CCTNAGG 1 cut(s) 909
EcoO109I RGGNCCY 1 cut(s) 913
EcoRI GAATTC 1 cut(s) 97
EcoRII CCWGG 3 cut(s) 744, 1099, 1114
EcoT14I CCWWGG 2 cut(s) 435, 1072
EcoT22I ATGCAT 1 cut(s) 474
ErhI CCWWGG 2 cut(s) 435, 1072
FaeI CATG 3 cut(s) 545, 1088, 1131
FaqI GGGAC 2 cut(s) 388, 868
FatI CATG 3 cut(s) 541, 1084, 1127
FauNDI CATATG 1 cut(s) 1042
FbaI TGATCA 1 cut(s) 730
Fnu4HI GCNGC 5 cut(s) 288, 452, 512, 824, 1136
FokI GGATG 5 cut(s) 80, 263, 761, 905, 1144
Fsp4HI GCNGC 5 cut(s) 288, 452, 512, 824, 1136
FspBI CTAG 5 cut(s) 87, 720, 884, 1073, 1215
GlaI GCGC 2 cut(s) 391, 1139
GluI GCNGC 5 cut(s) 288, 452, 512, 824, 1136
HaeIII GGCC 4 cut(s) 145, 597, 925, 1233
HapII CCGG 4 cut(s) 8, 137, 206, 448
HhaI GCGC 2 cut(s) 392, 1140
Hin1II CATG 3 cut(s) 545, 1088, 1131
Hin6I GCGC 2 cut(s) 390, 1138
HinP1I GCGC 2 cut(s) 390, 1138
HincII GTYRAC 1 cut(s) 524
HindII GTYRAC 1 cut(s) 524
HinfI GANTC 6 cut(s) 21, 516, 971, 1049, 1142, 1297
HpaII CCGG 4 cut(s) 8, 137, 206, 448
HphI GGTGA 2 cut(s) 83, 1194
Hpy166II GTNNAC 2 cut(s) 524, 1346
Hpy188I TCNGA 7 cut(s) 112, 193, 730, 970, 1167, 1183, 1296
Hpy188III TCNNGA 7 cut(s) 25, 551, 626, 706, 865, 908, 957
Hpy8I GTNNAC 2 cut(s) 524, 1346
HpyAV CCTTC 4 cut(s) 240, 557, 999, 1134
HpyCH4III ACNGT 5 cut(s) 179, 413, 528, 851, 965
HpyCH4IV ACGT 2 cut(s) 810, 1262
HpyF10VI GCNNNNNNNGC 4 cut(s) 313, 488, 785, 794
HpyF3I CTNAG 7 cut(s) 425, 695, 775, 909, 1164, 1197, 1227
HpySE526I ACGT 2 cut(s) 810, 1262
Hsp92II CATG 3 cut(s) 545, 1088, 1131
HspAI GCGC 2 cut(s) 390, 1138
Ksp22I TGATCA 1 cut(s) 730
Kzo9I GATC 5 cut(s) 73, 421, 730, 830, 1364
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 1 cut(s) 771
Lsp1109I GCAGC 4 cut(s) 274, 523, 810, 1147
LweI GCATC 5 cut(s) 151, 299, 962, 1118, 1122
MaeI CTAG 5 cut(s) 87, 720, 884, 1073, 1215
MaeII ACGT 2 cut(s) 810, 1262
MaeIII GTNAC 2 cut(s) 982, 1200
MalI GATC 5 cut(s) 75, 423, 732, 832, 1366
MbiI CCGCTC 1 cut(s) 1275
MboI GATC 5 cut(s) 73, 421, 730, 830, 1364
MboII GAAGA 8 cut(s) 174, 347, 350, 367, 539, 580, 624, 816
MflI RGATCY 1 cut(s) 830
MlsI TGGCCA 1 cut(s) 597
MluCI AATT 7 cut(s) 97, 230, 760, 818, 1177, 1255, 1311
MluNI TGGCCA 1 cut(s) 597
MlyI GAGTC 2 cut(s) 30, 1306
MmeI TCCRAC 3 cut(s) 38, 198, 478
MnlI CCTC 7 cut(s) 400, 600, 638, 762, 904, 1173, 1216
Mox20I TGGCCA 1 cut(s) 597
Mph1103I ATGCAT 1 cut(s) 474
MscI TGGCCA 1 cut(s) 597
Msp20I TGGCCA 1 cut(s) 597
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 4 cut(s) 8, 137, 206, 448
MspR9I CCNGG 4 cut(s) 207, 746, 1101, 1116
Mva1269I GAATGC 1 cut(s) 472
MvaI CCWGG 3 cut(s) 746, 1101, 1116
MvnI CGCG 2 cut(s) 390, 1140
MwoI GCNNNNNNNGC 4 cut(s) 313, 488, 785, 794
NciI CCSGG 1 cut(s) 207
NdeI CATATG 1 cut(s) 1042
NdeII GATC 5 cut(s) 73, 421, 730, 830, 1364
NlaIII CATG 3 cut(s) 545, 1088, 1131
NlaIV GGNNCC 3 cut(s) 6, 915, 1010
NmuCI GTSAC 1 cut(s) 1200
NsiI ATGCAT 1 cut(s) 474
PceI AGGCCT 1 cut(s) 925
PciSI GCTCTTC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 1306
PctI GAATGC 1 cut(s) 472
PfeI GAWTC 4 cut(s) 516, 971, 1049, 1142
PflMI CCANNNNNTGG 1 cut(s) 113
PkrI GCNGC 5 cut(s) 289, 453, 513, 825, 1137
PleI GAGTC 2 cut(s) 29, 1305
PpsI GAGTC 2 cut(s) 29, 1305
PpuMI RGGWCCY 1 cut(s) 913
PshAI GACNNNNGTC 2 cut(s) 20, 860
Psp5II RGGWCCY 1 cut(s) 913
Psp6I CCWGG 3 cut(s) 744, 1099, 1114
PspGI CCWGG 3 cut(s) 744, 1099, 1114
PspN4I GGNNCC 3 cut(s) 6, 915, 1010
PspPI GGNCC 4 cut(s) 203, 742, 913, 1231
PspPPI RGGWCCY 1 cut(s) 913
PstI CTGCAG 2 cut(s) 637, 828
PstNI CAGNNNCTG 1 cut(s) 1106
PsuI RGATCY 1 cut(s) 830
RsaI GTAC 3 cut(s) 223, 345, 1039
RsaNI GTAC 3 cut(s) 222, 344, 1038
Rsr2I CGGWCCG 1 cut(s) 203
RsrII CGGWCCG 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 552
SatI GCNGC 5 cut(s) 288, 452, 512, 824, 1136
Sau3AI GATC 5 cut(s) 73, 421, 730, 830, 1364
Sau96I GGNCC 4 cut(s) 203, 742, 913, 1231
SchI GAGTC 2 cut(s) 30, 1306
ScrFI CCNGG 4 cut(s) 207, 746, 1101, 1116
SfaNI GCATC 5 cut(s) 151, 299, 962, 1118, 1122
SfcI CTRYAG 2 cut(s) 633, 824
SgrAI CRCCGGYG 1 cut(s) 447
SinI GGWCC 3 cut(s) 203, 742, 913
SmiI ATTTAAAT 1 cut(s) 1032
SmlI CTYRAG 2 cut(s) 302, 834
SmoI CTYRAG 2 cut(s) 302, 834
Sse9I AATT 7 cut(s) 97, 230, 760, 818, 1177, 1255, 1311
SseBI AGGCCT 1 cut(s) 925
SsiI CCGC 4 cut(s) 388, 451, 1173, 1273
SspI AATATT 1 cut(s) 945
SspMI CTAG 5 cut(s) 87, 720, 884, 1073, 1215
StuI AGGCCT 1 cut(s) 925
StyD4I CCNGG 4 cut(s) 205, 744, 1099, 1114
StyI CCWWGG 2 cut(s) 435, 1072
SwaI ATTTAAAT 1 cut(s) 1032
TaaI ACNGT 5 cut(s) 179, 413, 528, 851, 965
TaiI ACGT 2 cut(s) 813, 1265
TaqI TCGA 1 cut(s) 707
TaqII GACCGA 2 cut(s) 191, 1323
TasI AATT 7 cut(s) 97, 230, 760, 818, 1177, 1255, 1311
TatI WGTACW 2 cut(s) 343, 1037
TauI GCSGC 1 cut(s) 454
TfiI GAWTC 4 cut(s) 516, 971, 1049, 1142
TscAI CASTG 5 cut(s) 436, 498, 533, 856, 1111
TseFI GTSAC 1 cut(s) 1200
TseI GCWGC 4 cut(s) 287, 511, 823, 1135
Tsp45I GTSAC 1 cut(s) 1200
TspDTI ATGAA 5 cut(s) 297, 530, 576, 675, 1116
TspGWI ACGGA 3 cut(s) 256, 282, 1341
TspRI CASTG 5 cut(s) 436, 498, 533, 856, 1111
Van91I CCANNNNNTGG 1 cut(s) 113
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 3 cut(s) 203, 742, 913
XapI RAATTY 2 cut(s) 97, 1177
XmaJI CCTAGG 1 cut(s) 1072
XspI CTAG 5 cut(s) 87, 720, 884, 1073, 1215
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.