pycom17g00380

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005343
Physical Location & Seq
Forward (+)
275120 .. 275584
465 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 465 bp
ATGGCTGAAACTAAGGACCAAGGCGAACCCACATTTCCAGCGAAGCGCAAACCCGATCTCAGCTGCGGTAACCAGCCCGACTGCCCCAACAAAACCCAGAAGCTTGAAGTTCCCGATGACAGTCCGTCGGCTGCCGAAGCTAAAAGTCAAACCCCAGAAAATTTCAAAAACAATTCCTCCGCTGGCGAAGAGAAAACCTGTAAGTTTGAAGCCAATGCTGACCTCGAAGACGAAGACGAAGAGGAAGATTACGAAGAAGAAGAAGAAGAAGATGAAAAGTCTAATGGGAAGGCGGAGATGGATCGCAAGGGGAAGGGGATTATGAGAGATGACAAGGGCAAAGGGAAACTGGTAGAAGAAGATGACGACGACAGCGACGATGATTCGAGCGACGGCGGAAGCGAATTCGAAGACGGAGACAGCGATTTGTCGGATCGATCCTCTTGCGGAGGTCGATTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

16.88

Weight (kDa)

4.11

Isoelectric Point (pI)

57.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 66, 180, 293, 396, 447
AclWI GGATC 3 cut(s) 309, 432, 441
AcsI RAATTY 2 cut(s) 160, 404
AgsI TTSAA 3 cut(s) 107, 166, 209
AhdI GACNNNNNGTC 1 cut(s) 124
AluBI AGCT 3 cut(s) 63, 103, 140
AluI AGCT 3 cut(s) 63, 103, 140
Alw26I GTCTC 1 cut(s) 411
AlwI GGATC 3 cut(s) 309, 432, 441
ApeKI GCWGC 2 cut(s) 63, 131
ApoI RAATTY 2 cut(s) 160, 404
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 408
AvaII GGWCC 1 cut(s) 16
BbsI GAAGAC 3 cut(s) 234, 240, 417
BbvI GCAGC 2 cut(s) 50, 118
BccI CCATC 1 cut(s) 292
BceAI ACGGC 1 cut(s) 409
BcgI CGANNNNNNTGC 2 cut(s) 426, 460
BcoDI GTCTC 1 cut(s) 411
BisI GCNGC 2 cut(s) 64, 132
BlsI GCNGC 2 cut(s) 65, 133
Bme18I GGWCC 1 cut(s) 16
BmeRI GACNNNNNGTC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 16
BpiI GAAGAC 3 cut(s) 234, 240, 417
Bpu14I TTCGAA 1 cut(s) 408
Bsa29I ATCGAT 1 cut(s) 436
BsaJI CCNNGG 1 cut(s) 19
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bse1I ACTGG 1 cut(s) 354
BseCI ATCGAT 1 cut(s) 436
BseDI CCNNGG 1 cut(s) 19
BseMII CTCAG 1 cut(s) 73
BseNI ACTGG 1 cut(s) 354
BseXI GCAGC 2 cut(s) 50, 118
BshVI ATCGAT 1 cut(s) 436
BsmAI GTCTC 1 cut(s) 411
Bsp119I TTCGAA 1 cut(s) 408
Bsp143I GATC 4 cut(s) 55, 301, 433, 437
BspACI CCGC 5 cut(s) 66, 180, 293, 396, 447
BspCNI CTCAG 1 cut(s) 72
BspDI ATCGAT 1 cut(s) 436
BspPI GGATC 3 cut(s) 309, 432, 441
BspT104I TTCGAA 1 cut(s) 408
BsrI ACTGG 1 cut(s) 354
BssECI CCNNGG 1 cut(s) 19
BssMI GATC 4 cut(s) 55, 301, 433, 437
BssT1I CCWWGG 1 cut(s) 19
Bst4CI ACNGT 1 cut(s) 122
Bst6I CTCTTC 2 cut(s) 183, 234
BstBI TTCGAA 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 2 cut(s) 12, 59
BstEII GGTNACC 1 cut(s) 68
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 4 cut(s) 58, 304, 436, 440
BstMAI GTCTC 1 cut(s) 411
BstMBI GATC 4 cut(s) 55, 301, 433, 437
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstPI GGTNACC 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 50, 118
BstV2I GAAGAC 3 cut(s) 234, 240, 417
Bsu15I ATCGAT 1 cut(s) 436
BsuTUI ATCGAT 1 cut(s) 436
Cac8I GCNNGC 1 cut(s) 184
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 16
ClaI ATCGAT 1 cut(s) 436
CviJI RGCY 7 cut(s) 5, 63, 76, 103, 131, 140, 212
CviKI_1 RGCY 7 cut(s) 5, 63, 76, 103, 131, 140, 212
DdeI CTNAG 2 cut(s) 12, 59
DpnI GATC 4 cut(s) 57, 303, 435, 439
DpnII GATC 4 cut(s) 55, 301, 433, 437
DriI GACNNNNNGTC 1 cut(s) 124
Eam1104I CTCTTC 2 cut(s) 183, 234
Eam1105I GACNNNNNGTC 1 cut(s) 124
EarI CTCTTC 2 cut(s) 183, 234
EciI GGCGGA 2 cut(s) 308, 411
Eco130I CCWWGG 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 16
Eco91I GGTNACC 1 cut(s) 68
EcoO65I GGTNACC 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 19
FaiI YATR 1 cut(s) 323
Fnu4HI GCNGC 2 cut(s) 64, 132
Fsp4HI GCNGC 2 cut(s) 64, 132
GlaI GCGC 1 cut(s) 47
GluI GCNGC 2 cut(s) 64, 132
HhaI GCGC 1 cut(s) 48
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 1 cut(s) 383
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 1 cut(s) 113
Hpy99I CGWCG 4 cut(s) 130, 371, 380, 395
HpyAV CCTTC 2 cut(s) 283, 307
HpyCH4III ACNGT 1 cut(s) 122
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HpyF3I CTNAG 2 cut(s) 12, 59
HspAI GCGC 1 cut(s) 46
Kzo9I GATC 4 cut(s) 55, 301, 433, 437
LpnPI CCDG 7 cut(s) 51, 86, 110, 168, 168, 211, 335
Lsp1109I GCAGC 2 cut(s) 50, 118
MaeIII GTNAC 1 cut(s) 68
MalI GATC 4 cut(s) 57, 303, 435, 439
MboI GATC 4 cut(s) 55, 301, 433, 437
MluCI AATT 3 cut(s) 160, 172, 404
MmeI TCCRAC 1 cut(s) 411
MnlI CCTC 5 cut(s) 187, 233, 235, 443, 451
MspA1I CMGCKG 2 cut(s) 63, 182
MwoI GCNNNNNNNGC 1 cut(s) 137
NdeII GATC 4 cut(s) 55, 301, 433, 437
NspV TTCGAA 1 cut(s) 408
PcsI WCGNNNNNNNCGW 4 cut(s) 372, 375, 399, 420
PfeI GAWTC 1 cut(s) 383
PkrI GCNGC 2 cut(s) 65, 133
PspEI GGTNACC 1 cut(s) 68
PspPI GGNCC 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 63
SatI GCNGC 2 cut(s) 64, 132
Sau3AI GATC 4 cut(s) 55, 301, 433, 437
Sau96I GGNCC 1 cut(s) 16
SetI ASST 6 cut(s) 65, 105, 142, 200, 225, 454
SfuI TTCGAA 1 cut(s) 408
SinI GGWCC 1 cut(s) 16
Sse9I AATT 3 cut(s) 160, 172, 404
SsiI CCGC 5 cut(s) 66, 180, 293, 396, 447
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 122
TaqI TCGA 5 cut(s) 225, 386, 408, 436, 454
TasI AATT 3 cut(s) 160, 172, 404
TfiI GAWTC 1 cut(s) 383
TseI GCWGC 2 cut(s) 63, 131
TspDTI ATGAA 1 cut(s) 288
TspGWI ACGGA 2 cut(s) 114, 429
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 2 cut(s) 160, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.