pycom17g01380

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002201
Physical Location & Seq
Reverse (-)
940209 .. 942697
2489 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
ATGCCTCCCACATATTTTCCTCTGCGGTGGGAGAGCACTGGAGACCAATGGTGGTACGCGTCTCCGATCGACTACGCAGCCGCTAACGGCCACTACGACTTGGTTCGAGAGCTCCTCCGCATAGACGGCAACCACCTCATCAAGCTCACATCTCTCCGCCGCATCCGCCGCCTCGAGACAGTGTGGGACGACGAAGCACAGTATGATGATGTAGCCAGGTGTCGTTCGCGCGTTGCGCGGAAGCTCTTCCACGAATGCGAGTCCAAGAAAGAAAAAACAAACTCTCTCATAAGGGCTGGATACGGCGGTTGGCTTATGTACACCGCCGCCTCCGCCGGGGAGCTTGGTTTTGTCCAAGAACTTCTCGAGAGAAACCCTCTGCTTGTTTTCGGGGAAGGAGAATATGGGGTGACTGATATTTTCTATGCTGCTGCTAGGAGCAAGAATGTTGAGGTTTTCAGGCTGCTTTTTGACTTTGCGGTTTCGCCGAGGTTTATGACGGGGAGCCGCGGAGAGCTGGAGGAGCACATTGGGGAGATTCCAAGTGTTTATAAACGGGAAATGGTTAACAGAGCGGTTCATGCAGCGGCGAGAGGCGGAAATTTGAGTATTTTGAAGGACCTTCTTTCGGATTGTGAGGATGTTTTGGCTTATAGGGATATTCAGGGATCAACTGTGCTCCATGCAGCGGCTGGGAAAGGAAGGGTAGAGGTAGTGAAGTACCTTGTGGCATCCTTTGATATCATAAACTCCACTGACCGCCAGGGCAACACAGCATTGCATGTTGCAGCTTCAAGGGGCCAACTTGCCGCAGCCGAAGCGCTTATATCTGCATCCCCGTCATCCATTATTGCACGAAACAACTCTGGAGAAACGTTTCTCCACAAGGCTATATCCGGCTTCCAAAGTCCTGCCTTCCGAAGACTGGACAGACAAATTCAGCTTCTGAAGCAACTGGTGAGTAAGAAAGCTTTCAATATCGAACACATCATCAATGCGAAAAACAATGAAGGACGGACTGCCCTTCACACGGCAATCATTGGGGATGTGCATTCCGATCTAGTCCAACTTCTGATGATTGCACAATCAATTGATGTGAATGCGCGCGACATGGATGGCATGACAGCGCTTGATTACCTTAGACAATGGCCTCGTTCAGCATCATCTGATATACTAATCAGGCAGCTGCTTTCAGCTGGGGGAATATTTGGTTGTGAAGATTACAATGCAAGAAAAGTGATTGCTTCGCGCTTGAAGATGCAAGGCGATGGAGGTAGTCCCGGAACATCATTTAGAATCTCTGACACGGAAATCTTCTTGTATACTGGCATTGAAAATGTATCAGATGCTACTGCTGATCGATCCGTAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5

Protein Analysis

458

Amino Acids

50.72

Weight (kDa)

6.62

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_4 PF13637 193 - 243 3e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 194 - 277 1.5e-15 Ankyrin repeats (3 copies)
Ank_4 PF13637 227 - 276 1e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 249 - 297 6.1e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 250 - 300 2.1e-06 Ankyrin repeats (3 copies)
Ank_5 PF13857 331 - 379 6.5e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AccBSI CCGCTC 1 cut(s) 575
AccI GTMKAC 1 cut(s) 1322
AccII CGCG 8 cut(s) 59, 229, 231, 238, 510, 1105, 1107, 1249
AclI AACGTT 1 cut(s) 875
AclWI GGATC 2 cut(s) 676, 1356
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 2 cut(s) 601, 936
AcuI CTGAAG 1 cut(s) 968
AfaI GTAC 3 cut(s) 56, 320, 722
AfeI AGCGCT 2 cut(s) 822, 1128
AfiI CCNNNNNNNGG 5 cut(s) 336, 628, 688, 925, 1030
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 5 cut(s) 616, 795, 976, 1255, 1334
AjnI CCWGG 2 cut(s) 215, 762
Alw21I GWGCWC 4 cut(s) 38, 114, 528, 681
Alw26I GTCTC 3 cut(s) 36, 66, 170
AlwI GGATC 2 cut(s) 676, 1356
AlwNI CAGNNNCTG 2 cut(s) 692, 946
Ama87I CYCGRG 2 cut(s) 173, 365
Aor51HI AGCGCT 2 cut(s) 822, 1128
AoxI GGCC 3 cut(s) 88, 799, 1148
ApoI RAATTY 2 cut(s) 601, 936
Asp700I GAANNNNTTC 3 cut(s) 245, 876, 971
AspLEI GCGC 7 cut(s) 231, 238, 823, 1105, 1107, 1129, 1251
AspS9I GGNCC 2 cut(s) 619, 799
AsuC2I CCSGG 2 cut(s) 337, 1281
AsuHPI GGTGA 2 cut(s) 421, 970
AvaI CYCGRG 2 cut(s) 173, 365
AvaII GGWCC 1 cut(s) 619
BaeI ACNNNNGTAYC 2 cut(s) 292, 325
BanII GRGCYC 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 928
Bbv12I GWGCWC 4 cut(s) 38, 114, 528, 681
BccI CCATC 2 cut(s) 1109, 1262
BceAI ACGGC 4 cut(s) 103, 142, 319, 1047
BciT130I CCWGG 2 cut(s) 217, 764
BciVI GTATCC 1 cut(s) 293
BcnI CCSGG 2 cut(s) 337, 1281
BcoDI GTCTC 3 cut(s) 36, 66, 170
BfaI CTAG 2 cut(s) 435, 1061
BfoI RGCGCY 2 cut(s) 824, 1130
BfuI GTATCC 1 cut(s) 293
Bme1390I CCNGG 4 cut(s) 217, 337, 764, 1281
Bme18I GGWCC 1 cut(s) 619
BmeT110I CYCGRG 2 cut(s) 173, 365
BmgT120I GGNCC 2 cut(s) 619, 799
BmiI GGNNCC 2 cut(s) 506, 800
BmrFI CCNGG 4 cut(s) 217, 337, 764, 1281
BmsI GCATC 6 cut(s) 171, 740, 842, 1169, 1248, 1336
BpiI GAAGAC 1 cut(s) 928
BplI GAGNNNNNCTC 4 cut(s) 99, 131, 361, 393
BpmI CTGGAG 3 cut(s) 60, 539, 888
BpuMI CCSGG 2 cut(s) 337, 1281
Bsa29I ATCGAT 1 cut(s) 1360
BsaI GGTCTC 1 cut(s) 36
BsaJI CCNNGG 4 cut(s) 336, 488, 508, 763
Bsc4I CCNNNNNNNGG 5 cut(s) 336, 628, 688, 925, 1030
Bse1I ACTGG 4 cut(s) 43, 930, 960, 1330
Bse3DI GCAATG 1 cut(s) 776
BseBI CCWGG 2 cut(s) 217, 764
BseCI ATCGAT 1 cut(s) 1360
BseDI CCNNGG 4 cut(s) 336, 488, 508, 763
BseGI GGATG 7 cut(s) 162, 646, 731, 833, 842, 1051, 1120
BseLI CCNNNNNNNGG 5 cut(s) 336, 628, 688, 925, 1030
BseMI GCAATG 1 cut(s) 776
BseNI ACTGG 4 cut(s) 43, 930, 960, 1330
BsePI GCGCGC 1 cut(s) 1103
BseRI GAGGAG 2 cut(s) 104, 536
BseYI CCCAGC 2 cut(s) 692, 1196
Bsh1236I CGCG 8 cut(s) 59, 229, 231, 238, 510, 1105, 1107, 1249
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 3 cut(s) 90, 801, 1150
BshVI ATCGAT 1 cut(s) 1360
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 4 cut(s) 38, 114, 528, 681
BsiHKCI CYCGRG 2 cut(s) 173, 365
BsiSI CCGG 3 cut(s) 336, 897, 1281
BslFI GGGAC 2 cut(s) 200, 1263
BslI CCNNNNNNNGG 5 cut(s) 336, 628, 688, 925, 1030
BsmAI GTCTC 3 cut(s) 36, 66, 170
BsmBI CGTCTC 1 cut(s) 66
BsmFI GGGAC 2 cut(s) 200, 1263
BsmI GAATGC 3 cut(s) 260, 1051, 1105
BsnI GGCC 3 cut(s) 90, 801, 1150
Bso31I GGTCTC 1 cut(s) 36
BsoBI CYCGRG 2 cut(s) 173, 365
Bsp1286I GDGCHC 4 cut(s) 38, 114, 528, 681
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 5 cut(s) 66, 668, 1057, 1357, 1361
BspANI GGCC 3 cut(s) 90, 801, 1150
BspDI ATCGAT 1 cut(s) 1360
BspFNI CGCG 8 cut(s) 59, 229, 231, 238, 510, 1105, 1107, 1249
BspLI GGNNCC 2 cut(s) 506, 800
BspPI GGATC 2 cut(s) 676, 1356
BspQI GCTCTTC 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 36
BsrBI CCGCTC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 776
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 4 cut(s) 43, 930, 960, 1330
BssECI CCNNGG 4 cut(s) 336, 488, 508, 763
BssHII GCGCGC 1 cut(s) 1103
BssMI GATC 5 cut(s) 66, 668, 1057, 1357, 1361
BssNAI GTATAC 1 cut(s) 1323
Bst1107I GTATAC 1 cut(s) 1323
Bst2UI CCWGG 2 cut(s) 217, 764
Bst4CI ACNGT 3 cut(s) 181, 201, 676
Bst6I CTCTTC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 318
BstC8I GCNNGC 1 cut(s) 1105
BstDEI CTNAG 1 cut(s) 1139
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 7 cut(s) 162, 646, 731, 833, 842, 1051, 1120
BstFNI CGCG 8 cut(s) 59, 229, 231, 238, 510, 1105, 1107, 1249
BstH2I RGCGCY 2 cut(s) 824, 1130
BstHHI GCGC 7 cut(s) 231, 238, 823, 1105, 1107, 1129, 1251
BstKTI GATC 5 cut(s) 69, 671, 1060, 1360, 1364
BstMAI GTCTC 3 cut(s) 36, 66, 170
BstMBI GATC 5 cut(s) 66, 668, 1057, 1357, 1361
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 9 cut(s) 126, 165, 168, 235, 332, 523, 581, 818, 949
BstNI CCWGG 2 cut(s) 217, 764
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 4 cut(s) 215, 335, 762, 1279
BstUI CGCG 8 cut(s) 59, 229, 231, 238, 510, 1105, 1107, 1249
BstV2I GAAGAC 1 cut(s) 928
BstZ17I GTATAC 1 cut(s) 1323
Bsu15I ATCGAT 1 cut(s) 1360
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 3 cut(s) 90, 801, 1150
BsuTUI ATCGAT 1 cut(s) 1360
BtgI CCRYGG 1 cut(s) 508
BtgZI GCGATG 1 cut(s) 1281
BtsCI GGATG 7 cut(s) 162, 646, 731, 833, 842, 1051, 1120
BtsIMutI CAGTG 3 cut(s) 36, 186, 753
Cac8I GCNNGC 1 cut(s) 1105
CaiI CAGNNNCTG 2 cut(s) 692, 946
CfoI GCGC 7 cut(s) 231, 238, 823, 1105, 1107, 1129, 1251
Cfr13I GGNCC 2 cut(s) 619, 799
Cfr42I CCGCGG 1 cut(s) 511
ClaI ATCGAT 1 cut(s) 1360
CseI GACGC 1 cut(s) 48
Csp6I GTAC 3 cut(s) 55, 319, 721
CviAII CATG 5 cut(s) 581, 683, 782, 1111, 1120
CviQI GTAC 3 cut(s) 55, 319, 721
DdeI CTNAG 1 cut(s) 1139
DpnI GATC 5 cut(s) 68, 670, 1059, 1359, 1363
DpnII GATC 5 cut(s) 66, 668, 1057, 1357, 1361
EaeI YGGCCR 1 cut(s) 88
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 4 cut(s) 146, 155, 322, 612
Ecl136II GAGCTC 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 114
Eco31I GGTCTC 1 cut(s) 36
Eco32I GATATC 1 cut(s) 742
Eco47I GGWCC 1 cut(s) 619
Eco47III AGCGCT 2 cut(s) 822, 1128
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 968
Eco88I CYCGRG 2 cut(s) 173, 365
EcoICRI GAGCTC 1 cut(s) 112
EcoO109I RGGNCCY 1 cut(s) 619
EcoRII CCWGG 2 cut(s) 215, 762
EcoRV GATATC 1 cut(s) 742
EcoT38I GRGCYC 1 cut(s) 114
Esp3I CGTCTC 1 cut(s) 66
FaeI CATG 5 cut(s) 584, 686, 785, 1114, 1123
FaqI GGGAC 2 cut(s) 200, 1263
FatI CATG 5 cut(s) 580, 682, 781, 1110, 1119
FblI GTMKAC 1 cut(s) 1322
FokI GGATG 7 cut(s) 149, 653, 718, 820, 829, 1058, 1127
FriOI GRGCYC 1 cut(s) 114
FspBI CTAG 2 cut(s) 435, 1061
GlaI GCGC 7 cut(s) 230, 237, 822, 1104, 1106, 1128, 1250
GsaI CCCAGC 2 cut(s) 696, 1200
GsuI CTGGAG 3 cut(s) 60, 539, 888
HaeII RGCGCY 2 cut(s) 824, 1130
HaeIII GGCC 3 cut(s) 90, 801, 1150
HapII CCGG 3 cut(s) 336, 897, 1281
HgaI GACGC 1 cut(s) 48
HhaI GCGC 7 cut(s) 231, 238, 823, 1105, 1107, 1129, 1251
Hin1II CATG 5 cut(s) 584, 686, 785, 1114, 1123
Hin6I GCGC 7 cut(s) 229, 236, 821, 1103, 1105, 1127, 1249
HinP1I GCGC 7 cut(s) 229, 236, 821, 1103, 1105, 1127, 1249
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 1 cut(s) 969
HinfI GANTC 3 cut(s) 260, 538, 1296
HpaI GTTAAC 1 cut(s) 568
HpaII CCGG 3 cut(s) 336, 897, 1281
HphI GGTGA 2 cut(s) 421, 970
Hpy166II GTNNAC 3 cut(s) 321, 568, 1323
Hpy188I TCNGA 9 cut(s) 66, 631, 920, 948, 1057, 1074, 1168, 1303, 1345
Hpy188III TCNNGA 5 cut(s) 107, 175, 365, 367, 867
Hpy8I GTNNAC 3 cut(s) 321, 568, 1323
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 7 cut(s) 389, 610, 632, 696, 925, 1004, 1034
HpyCH4III ACNGT 3 cut(s) 181, 201, 676
HpyCH4IV ACGT 1 cut(s) 875
HpyF10VI GCNNNNNNNGC 9 cut(s) 126, 165, 168, 235, 332, 523, 581, 818, 949
HpyF3I CTNAG 1 cut(s) 1139
HpySE526I ACGT 1 cut(s) 875
Hsp92II CATG 5 cut(s) 584, 686, 785, 1114, 1123
HspAI GCGC 7 cut(s) 229, 236, 821, 1103, 1105, 1127, 1249
KspAI GTTAAC 1 cut(s) 568
KspI CCGCGG 1 cut(s) 511
Kzo9I GATC 5 cut(s) 66, 668, 1057, 1357, 1361
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 6 cut(s) 117, 340, 438, 504, 523, 684
LweI GCATC 6 cut(s) 171, 740, 842, 1169, 1248, 1336
MaeI CTAG 2 cut(s) 435, 1061
MaeII ACGT 1 cut(s) 875
MaeIII GTNAC 1 cut(s) 409
MalI GATC 5 cut(s) 68, 670, 1059, 1359, 1363
MbiI CCGCTC 1 cut(s) 575
MboI GATC 5 cut(s) 66, 668, 1057, 1357, 1361
MboII GAAGA 5 cut(s) 238, 933, 1229, 1267, 1306
MfeI CAATTG 1 cut(s) 1089
MhlI GDGCHC 4 cut(s) 38, 114, 528, 681
MluCI AATT 3 cut(s) 601, 936, 1089
MluI ACGCGT 1 cut(s) 57
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 1090
MroXI GAANNNNTTC 3 cut(s) 245, 876, 971
MseI TTAA 1 cut(s) 567
MspA1I CMGCKG 5 cut(s) 510, 587, 689, 1186, 1196
MspI CCGG 3 cut(s) 336, 897, 1281
MspR9I CCNGG 4 cut(s) 217, 337, 764, 1281
MunI CAATTG 1 cut(s) 1089
Mva1269I GAATGC 3 cut(s) 260, 1051, 1105
MvaI CCWGG 2 cut(s) 217, 764
MvnI CGCG 8 cut(s) 59, 229, 231, 238, 510, 1105, 1107, 1249
MwoI GCNNNNNNNGC 9 cut(s) 126, 165, 168, 235, 332, 523, 581, 818, 949
NciI CCSGG 2 cut(s) 337, 1281
NdeII GATC 5 cut(s) 66, 668, 1057, 1357, 1361
NlaIII CATG 5 cut(s) 584, 686, 785, 1114, 1123
NlaIV GGNNCC 2 cut(s) 506, 800
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 1 cut(s) 785
PaeR7I CTCGAG 2 cut(s) 173, 365
PauI GCGCGC 1 cut(s) 1103
PciSI GCTCTTC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 3 cut(s) 260, 1051, 1105
PdmI GAANNNNTTC 3 cut(s) 245, 876, 971
PfeI GAWTC 2 cut(s) 538, 1296
PfoI TCCNGGA 1 cut(s) 1279
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 619
PsiI TTATAA 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 114
Psp1406I AACGTT 1 cut(s) 875
Psp5II RGGWCCY 1 cut(s) 619
Psp6I CCWGG 2 cut(s) 215, 762
PspFI CCCAGC 2 cut(s) 692, 1196
PspGI CCWGG 2 cut(s) 215, 762
PspN4I GGNNCC 2 cut(s) 506, 800
PspPI GGNCC 2 cut(s) 619, 799
PspPPI RGGWCCY 1 cut(s) 619
PstNI CAGNNNCTG 2 cut(s) 692, 946
PteI GCGCGC 1 cut(s) 1103
PvuI CGATCG 1 cut(s) 69
PvuII CAGCTG 2 cut(s) 1186, 1196
RsaI GTAC 3 cut(s) 56, 320, 722
RsaNI GTAC 3 cut(s) 55, 319, 721
SacI GAGCTC 1 cut(s) 114
SacII CCGCGG 1 cut(s) 511
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 567
Sau3AI GATC 5 cut(s) 66, 668, 1057, 1357, 1361
Sau96I GGNCC 2 cut(s) 619, 799
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 4 cut(s) 217, 337, 764, 1281
SduI GDGCHC 4 cut(s) 38, 114, 528, 681
SfaNI GCATC 6 cut(s) 171, 740, 842, 1169, 1248, 1336
Sfr274I CTCGAG 2 cut(s) 173, 365
Sfr303I CCGCGG 1 cut(s) 511
SgrBI CCGCGG 1 cut(s) 511
SinI GGWCC 1 cut(s) 619
SlaI CTCGAG 2 cut(s) 173, 365
SmlI CTYRAG 2 cut(s) 173, 365
SmoI CTYRAG 2 cut(s) 173, 365
Sse9I AATT 3 cut(s) 601, 936, 1089
SspI AATATT 1 cut(s) 1206
SspMI CTAG 2 cut(s) 435, 1061
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 4 cut(s) 215, 335, 762, 1279
TaaI ACNGT 3 cut(s) 181, 201, 676
TaiI ACGT 1 cut(s) 878
TaqI TCGA 6 cut(s) 69, 106, 174, 366, 981, 1360
TasI AATT 3 cut(s) 601, 936, 1089
TatI WGTACW 1 cut(s) 318
TauI GCSGC 8 cut(s) 83, 162, 171, 329, 510, 590, 692, 812
TfiI GAWTC 2 cut(s) 538, 1296
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TscAI CASTG 3 cut(s) 43, 186, 760
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 2 cut(s) 569, 1023
TspGWI ACGGA 3 cut(s) 1030, 1322, 1354
TspRI CASTG 3 cut(s) 43, 186, 760
VpaK11BI GGWCC 1 cut(s) 619
XapI RAATTY 2 cut(s) 601, 936
XceI RCATGY 1 cut(s) 785
XcmI CCANNNNNNNNNTGG 1 cut(s) 689
XhoI CTCGAG 2 cut(s) 173, 365
XmiI GTMKAC 1 cut(s) 1322
XmnI GAANNNNTTC 3 cut(s) 245, 876, 971
XspI CTAG 2 cut(s) 435, 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.