pycom17g25630

Transcriptional activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002428
Physical Location & Seq
Forward (+)
23737409 .. 23739986
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1338 bp
ATGGACTCTCTGGACTATGAAGCACTCATAAATGCTAATGTCAAAGAAATATCTGATGCAATCAAGGAGCGAGGGATGAACAACTTGCTAGCGGAACGAATGCAGGAATTCCTAAAGCGACTGGTTAGAGAACATGAAAGCATCAATCTGGAATGGTTAAGAGATGTTCCCCCTGATAAAGCAAAGGATTATCTATTAAGCATACGAGGATTGGGCTTGAAAAGTGTGGAGTGCATCCGGCTTTTAACACTTCACCACCTTGCTTTCCCGGTTGACACAAATGTTGGAAGGATAGCAGTAAGACTGGGATGGGTCCCCCTTCAACCATTACCCGAATCACTGCAGTTGCACCTCTTAGAACTGTACCCAATGCTGGAGTCGATTCAAAAGTATTTATGGCCAAGATTGTGCAAACTTGATCAACTAACCCTTTACGAGCTGCACTACCAGATGATTACATTTGGAAAGGTATTTTGTACAAAGAGTAAACCGAATTGCAATGCATGTCCAATGAGAGGAGAATGTAGACACTTCGCAAGTGCTTTTGCAAGTGCAAGACTTGCCCTGCCAGGGCCAGAAGAGAAGAGAATTGTCAGTTCAAATGTTTCCATTGAACCCGAGATAAATCCCGCCATAGCTGTCACTCCTATGTCACTGCCTCCGCCTGAGAACAACTCACTCCAGATAGCAGGTACTGTAACTAAAGAGTGTGAACCAATAATCGAAGAGCCAGCAACGCCAGAACAAGAGTTGACAGAGCTATCAGAAAGTGAAATTGAGGACTTCTTCTATGAAGATCCTGATGAGATTCCTACCATCAAACTCAACATGGAAGAGTTCACATCGACATTACAAAACTATATGCAAGAGAAGAAGCTCCAAGAAGGTGACATGTCCAAAGCCTTAGTTGCATTGAATTCAGAAGCTACTTCTATCCCTACGCCCAAGCTGAAGAATGTCAGTAGACTACGAACGGAGCACCAAGTGTATGAACTACCTGATTCGCATCCACTCTTGGAAGGGATGGATAGACGGGAACCAGATGATCCAAGCCCATACCTTCTAGCTATATGGACACCAGGTAAGCTCAAGAAATCACAGAGAACTGAGATGCATTGTTTAGAAGTGAGTATCTTGTACATTTTGCCAAGTGATGCATTGATTCTGCATCACGGTATACCAGGGGAAACTGCAAATTCAATTCAACAACCAGAAAGCAGGTGTGGCTCCCAAGATCAAAACAAACTGTGCAATGAGAAGACATGCTTTTCATGCAATAGTATAAGAGAAGAAAATTTGCAAACAGTCAGAGGGACGATTTTGGTGAGGAAAATTTAA

Protein Analysis

446

Amino Acids

50.58

Weight (kDa)

5.24

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Perm-CXXC PF15629 404 - 435 1.8e-13 Permuted single zf-CXXC unit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1209
Acc36I ACCTGC 2 cut(s) 680, 1209
AccI GTMKAC 3 cut(s) 526, 964, 1177
AciI CCGC 3 cut(s) 92, 630, 662
AclWI GGATC 2 cut(s) 791, 1040
AcoI YGGCCR 1 cut(s) 398
AcsI RAATTY 5 cut(s) 107, 916, 1195, 1294, 1332
AcuI CTGAAG 1 cut(s) 971
AdeI CACNNNGTG 1 cut(s) 985
AfaI GTAC 4 cut(s) 365, 478, 694, 1139
AfiI CCNNNNNNNGG 3 cut(s) 373, 515, 570
AflIII ACRYGT 1 cut(s) 891
AgsI TTSAA 8 cut(s) 220, 323, 386, 600, 614, 916, 1200, 1205
AjnI CCWGG 3 cut(s) 568, 1078, 1180
AluBI AGCT 8 cut(s) 439, 638, 760, 877, 926, 949, 1067, 1087
AluI AGCT 8 cut(s) 439, 638, 760, 877, 926, 949, 1067, 1087
Alw21I GWGCWC 1 cut(s) 981
AlwI GGATC 2 cut(s) 791, 1040
AlwNI CAGNNNCTG 1 cut(s) 695
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 2 cut(s) 398, 572
ApeKI GCWGC 1 cut(s) 439
ApoI RAATTY 5 cut(s) 107, 916, 1195, 1294, 1332
AspS9I GGNCC 2 cut(s) 313, 572
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 3 cut(s) 245, 899, 1336
AsuNHI GCTAGC 1 cut(s) 88
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 1 cut(s) 313
BalI TGGCCA 1 cut(s) 400
BbsI GAAGAC 1 cut(s) 1265
Bbv12I GWGCWC 1 cut(s) 981
BbvI GCAGC 1 cut(s) 426
BccI CCATC 3 cut(s) 303, 824, 1018
BciT130I CCWGG 3 cut(s) 570, 1080, 1182
BclI TGATCA 1 cut(s) 418
BcnI CCSGG 1 cut(s) 269
BfaI CTAG 2 cut(s) 89, 1064
BfmI CTRYAG 1 cut(s) 341
BfuAI ACCTGC 2 cut(s) 680, 1209
BisI GCNGC 1 cut(s) 440
BlsI GCNGC 1 cut(s) 441
Bme1390I CCNGG 4 cut(s) 269, 570, 1080, 1182
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 2 cut(s) 313, 572
BmiI GGNNCC 4 cut(s) 314, 315, 1038, 1228
BmrFI CCNGG 4 cut(s) 269, 570, 1080, 1182
BmrI ACTGGG 1 cut(s) 314
BmsI GCATC 7 cut(s) 46, 150, 243, 1015, 1101, 1144, 1177
BmtI GCTAGC 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 1265
BplI GAGNNNNNCTC 2 cut(s) 659, 691
BpmI CTGGAG 2 cut(s) 395, 665
BpuEI CTTGAG 1 cut(s) 1073
BpuMI CCSGG 1 cut(s) 269
BsaBI GATNNNNATC 1 cut(s) 1005
BsaJI CCNNGG 2 cut(s) 569, 1181
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 515, 570
Bse1I ACTGG 2 cut(s) 126, 309
Bse3DI GCAATG 2 cut(s) 505, 1258
Bse8I GATNNNNATC 1 cut(s) 1005
BseBI CCWGG 3 cut(s) 570, 1080, 1182
BseDI CCNNGG 2 cut(s) 569, 1181
BseGI GGATG 5 cut(s) 81, 234, 314, 1006, 1029
BseJI GATNNNNATC 1 cut(s) 1005
BseLI CCNNNNNNNGG 3 cut(s) 373, 515, 570
BseMI GCAATG 2 cut(s) 505, 1258
BseMII CTCAG 2 cut(s) 657, 1098
BseNI ACTGG 2 cut(s) 126, 309
BseRI GAGGAG 1 cut(s) 531
BseXI GCAGC 1 cut(s) 426
BsgI GTGCAG 1 cut(s) 425
BshFI GGCC 2 cut(s) 400, 574
BsiHKAI GWGCWC 1 cut(s) 981
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 2 cut(s) 238, 269
BslFI GGGAC 2 cut(s) 299, 1327
BslI CCNNNNNNNGG 3 cut(s) 373, 515, 570
BsmFI GGGAC 2 cut(s) 299, 1327
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 2 cut(s) 400, 574
BsoBI CYCGRG 1 cut(s) 617
Bsp1286I GDGCHC 1 cut(s) 981
Bsp1407I TGTACA 2 cut(s) 476, 1137
Bsp143I GATC 4 cut(s) 418, 796, 1045, 1234
BspACI CCGC 3 cut(s) 92, 630, 662
BspANI GGCC 2 cut(s) 400, 574
BspCNI CTCAG 2 cut(s) 658, 1099
BspLI GGNNCC 4 cut(s) 314, 315, 1038, 1228
BspMAI CTGCAG 1 cut(s) 345
BspMI ACCTGC 2 cut(s) 680, 1209
BspOI GCTAGC 1 cut(s) 92
BspPI GGATC 2 cut(s) 791, 1040
BspQI GCTCTTC 1 cut(s) 720
BsrDI GCAATG 2 cut(s) 505, 1258
BsrGI TGTACA 2 cut(s) 476, 1137
BsrI ACTGG 2 cut(s) 126, 309
BssECI CCNNGG 2 cut(s) 569, 1181
BssMI GATC 4 cut(s) 418, 796, 1045, 1234
BssNAI GTATAC 1 cut(s) 1178
Bst1107I GTATAC 1 cut(s) 1178
Bst2UI CCWGG 3 cut(s) 570, 1080, 1182
Bst4CI ACNGT 5 cut(s) 363, 697, 1175, 1248, 1306
Bst6I CTCTTC 4 cut(s) 573, 578, 720, 828
BstAPI GCANNNNNTGC 1 cut(s) 560
BstAUI TGTACA 2 cut(s) 476, 1137
BstC8I GCNNGC 2 cut(s) 90, 732
BstDEI CTNAG 4 cut(s) 355, 666, 904, 1107
BstF5I GGATG 5 cut(s) 81, 234, 314, 1006, 1029
BstKTI GATC 4 cut(s) 421, 799, 1048, 1237
BstMBI GATC 4 cut(s) 418, 796, 1045, 1234
BstMWI GCNNNNNNNGC 5 cut(s) 560, 736, 908, 1224, 1272
BstNI CCWGG 3 cut(s) 570, 1080, 1182
BstNSI RCATGY 3 cut(s) 507, 895, 1266
BstSCI CCNGG 4 cut(s) 267, 568, 1078, 1180
BstSFI CTRYAG 1 cut(s) 341
BstV1I GCAGC 1 cut(s) 426
BstV2I GAAGAC 1 cut(s) 1265
BstX2I RGATCY 1 cut(s) 796
BstYI RGATCY 1 cut(s) 796
BstZ17I GTATAC 1 cut(s) 1178
BsuRI GGCC 2 cut(s) 400, 574
BtsCI GGATG 5 cut(s) 81, 234, 314, 1006, 1029
BtsI GCAGTG 2 cut(s) 338, 653
BtsIMutI CAGTG 2 cut(s) 338, 653
BveI ACCTGC 2 cut(s) 680, 1209
Cac8I GCNNGC 2 cut(s) 90, 732
CaiI CAGNNNCTG 1 cut(s) 695
Cfr13I GGNCC 2 cut(s) 313, 572
CsiI ACCWGGT 1 cut(s) 1078
Csp6I GTAC 4 cut(s) 364, 477, 693, 1138
CviAII CATG 6 cut(s) 134, 504, 829, 892, 1263, 1272
CviQI GTAC 4 cut(s) 364, 477, 693, 1138
DdeI CTNAG 4 cut(s) 355, 666, 904, 1107
DpnI GATC 4 cut(s) 420, 798, 1047, 1236
DpnII GATC 4 cut(s) 418, 796, 1045, 1234
DraIII CACNNNGTG 1 cut(s) 985
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 4 cut(s) 573, 578, 720, 828
EarI CTCTTC 4 cut(s) 573, 578, 720, 828
EciI GGCGGA 1 cut(s) 651
Eco47I GGWCC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 971
Eco88I CYCGRG 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 313
EcoRI GAATTC 2 cut(s) 107, 916
EcoRII CCWGG 3 cut(s) 568, 1078, 1180
EcoT22I ATGCAT 3 cut(s) 505, 1116, 1159
FaeI CATG 6 cut(s) 137, 507, 832, 895, 1266, 1275
FalI AAGNNNNNCTT 2 cut(s) 1250, 1282
FaqI GGGAC 2 cut(s) 299, 1327
FatI CATG 6 cut(s) 133, 503, 828, 891, 1262, 1271
FauI CCCGC 1 cut(s) 637
FbaI TGATCA 1 cut(s) 418
FblI GTMKAC 3 cut(s) 526, 964, 1177
Fnu4HI GCNGC 1 cut(s) 440
FokI GGATG 5 cut(s) 88, 221, 321, 993, 1036
Fsp4HI GCNGC 1 cut(s) 440
FspBI CTAG 2 cut(s) 89, 1064
GluI GCNGC 1 cut(s) 440
GsuI CTGGAG 2 cut(s) 395, 665
HaeIII GGCC 2 cut(s) 400, 574
HapII CCGG 2 cut(s) 238, 269
Hin1II CATG 6 cut(s) 137, 507, 832, 895, 1266, 1275
HincII GTYRAC 2 cut(s) 274, 753
HindII GTYRAC 2 cut(s) 274, 753
HinfI GANTC 7 cut(s) 5, 335, 377, 382, 808, 1001, 1162
HpaII CCGG 2 cut(s) 238, 269
HphI GGTGA 3 cut(s) 245, 899, 1336
Hpy166II GTNNAC 8 cut(s) 274, 488, 527, 713, 753, 840, 965, 1178
Hpy188I TCNGA 4 cut(s) 55, 766, 922, 1310
Hpy188III TCNNGA 5 cut(s) 11, 149, 682, 800, 1090
Hpy8I GTNNAC 8 cut(s) 274, 488, 527, 713, 753, 840, 965, 1178
HpyAV CCTTC 5 cut(s) 282, 329, 878, 1013, 1070
HpyCH4III ACNGT 5 cut(s) 363, 697, 1175, 1248, 1306
HpyF10VI GCNNNNNNNGC 5 cut(s) 560, 736, 908, 1224, 1272
HpyF3I CTNAG 4 cut(s) 355, 666, 904, 1107
Hsp92II CATG 6 cut(s) 137, 507, 832, 895, 1266, 1275
KflI GGGWCCC 1 cut(s) 313
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 4 cut(s) 418, 796, 1045, 1234
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 4 cut(s) 67, 882, 976, 1232
Lsp1109I GCAGC 1 cut(s) 426
LweI GCATC 7 cut(s) 46, 150, 243, 1015, 1101, 1144, 1177
MabI ACCWGGT 1 cut(s) 1078
MaeI CTAG 2 cut(s) 89, 1064
MaeIII GTNAC 4 cut(s) 640, 651, 697, 887
MalI GATC 4 cut(s) 420, 798, 1047, 1236
MboI GATC 4 cut(s) 418, 796, 1045, 1234
MflI RGATCY 1 cut(s) 796
MhlI GDGCHC 1 cut(s) 981
MlsI TGGCCA 1 cut(s) 400
MluCI AATT 9 cut(s) 107, 493, 588, 774, 916, 1195, 1200, 1294, 1332
MluNI TGGCCA 1 cut(s) 400
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 1 cut(s) 265
MnlI CCTC 8 cut(s) 65, 200, 362, 509, 669, 772, 1304, 1320
Mox20I TGGCCA 1 cut(s) 400
Mph1103I ATGCAT 3 cut(s) 505, 1116, 1159
MscI TGGCCA 1 cut(s) 400
MseI TTAA 4 cut(s) 158, 197, 245, 1336
MslI CAYNNNNRTG 1 cut(s) 647
Msp20I TGGCCA 1 cut(s) 400
MspI CCGG 2 cut(s) 238, 269
MspR9I CCNGG 4 cut(s) 269, 570, 1080, 1182
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 3 cut(s) 570, 1080, 1182
MwoI GCNNNNNNNGC 5 cut(s) 560, 736, 908, 1224, 1272
NciI CCSGG 1 cut(s) 269
NdeII GATC 4 cut(s) 418, 796, 1045, 1234
NheI GCTAGC 1 cut(s) 88
NlaIII CATG 6 cut(s) 137, 507, 832, 895, 1266, 1275
NlaIV GGNNCC 4 cut(s) 314, 315, 1038, 1228
NmuCI GTSAC 3 cut(s) 640, 651, 887
NsiI ATGCAT 3 cut(s) 505, 1116, 1159
NspI RCATGY 3 cut(s) 507, 895, 1266
PaqCI CACCTGC 1 cut(s) 1209
PciI ACATGT 1 cut(s) 891
PciSI GCTCTTC 1 cut(s) 720
PctI GAATGC 1 cut(s) 105
PfeI GAWTC 5 cut(s) 335, 382, 808, 1001, 1162
PkrI GCNGC 1 cut(s) 441
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
PpuMI RGGWCCY 1 cut(s) 313
PscI ACATGT 1 cut(s) 891
Psp5II RGGWCCY 1 cut(s) 313
Psp6I CCWGG 3 cut(s) 568, 1078, 1180
PspGI CCWGG 3 cut(s) 568, 1078, 1180
PspN4I GGNNCC 4 cut(s) 314, 315, 1038, 1228
PspPI GGNCC 2 cut(s) 313, 572
PspPPI RGGWCCY 1 cut(s) 313
PstI CTGCAG 1 cut(s) 345
PstNI CAGNNNCTG 1 cut(s) 695
PsuI RGATCY 1 cut(s) 796
RsaI GTAC 4 cut(s) 365, 478, 694, 1139
RsaNI GTAC 4 cut(s) 364, 477, 693, 1138
RseI CAYNNNNRTG 1 cut(s) 647
SapI GCTCTTC 1 cut(s) 720
SaqAI TTAA 4 cut(s) 158, 197, 245, 1336
SatI GCNGC 1 cut(s) 440
Sau3AI GATC 4 cut(s) 418, 796, 1045, 1234
Sau96I GGNCC 2 cut(s) 313, 572
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 4 cut(s) 269, 570, 1080, 1182
SduI GDGCHC 1 cut(s) 981
SexAI ACCWGGT 1 cut(s) 1078
SfaNI GCATC 7 cut(s) 46, 150, 243, 1015, 1101, 1144, 1177
SfcI CTRYAG 1 cut(s) 341
SinI GGWCC 1 cut(s) 313
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 1088
SmoI CTYRAG 1 cut(s) 1088
Sse9I AATT 9 cut(s) 107, 493, 588, 774, 916, 1195, 1200, 1294, 1332
SsiI CCGC 3 cut(s) 92, 630, 662
SspMI CTAG 2 cut(s) 89, 1064
StyD4I CCNGG 4 cut(s) 267, 568, 1078, 1180
TaaI ACNGT 5 cut(s) 363, 697, 1175, 1248, 1306
TaqI TCGA 3 cut(s) 380, 723, 845
TasI AATT 9 cut(s) 107, 493, 588, 774, 916, 1195, 1200, 1294, 1332
TatI WGTACW 2 cut(s) 476, 1137
TfiI GAWTC 5 cut(s) 335, 382, 808, 1001, 1162
Tru1I TTAA 4 cut(s) 158, 197, 245, 1336
Tru9I TTAA 4 cut(s) 158, 197, 245, 1336
TscAI CASTG 2 cut(s) 345, 660
TseFI GTSAC 3 cut(s) 640, 651, 887
TseI GCWGC 1 cut(s) 439
Tsp45I GTSAC 3 cut(s) 640, 651, 887
TspDTI ATGAA 6 cut(s) 33, 92, 150, 807, 1005, 1260
TspGWI ACGGA 1 cut(s) 989
TspRI CASTG 2 cut(s) 345, 660
VpaK11BI GGWCC 1 cut(s) 313
XapI RAATTY 5 cut(s) 107, 916, 1195, 1294, 1332
XceI RCATGY 3 cut(s) 507, 895, 1266
XmiI GTMKAC 3 cut(s) 526, 964, 1177
XspI CTAG 2 cut(s) 89, 1064
Zsp2I ATGCAT 3 cut(s) 505, 1116, 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.