RchiOBHm_Chr5g0056871

Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004109
Physical Location & Seq
Reverse (-)
60659595 .. 60661176
1582 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 987 bp
ATGGATGGGATGGGTGCATCCGGAAACCATGATCTTAGAGGTCACAGCGTCATTAATGACTTTGAGTTACAAGATTTCATAGATGATGCAAACTTCGACCAGTTCATCGATCTTATTCGTGGAGAAAATGAAGATCCAGCAGCTCAGTTTAATCTTGACCTTACCAGCGGGTCATTCTTTGATAACCAACTTTGTCCAACCACTGATACGGTCAGTTTTTTTGGTTTTGATCATAATATTACAATCCTATGCGATCCACCTCCTTTTCTTAGTACATTACCGAGTTTTGGTGGAGAAATGAACGACATTGGCGAAGAAGATAATGATGGGGGGGATTCGTGTGGGACAACGACAACAACAACAACAACAACCACCACAACCACAGCCACAACGACGAAGAAGCCAAAGGTTGATCGGTCGAGAACCTTGGTTTCGGAAAGAAGGAGAAGGGGAAGGATGAAGGAAAAACTCTATGCATTGCGTTCCTTGGTTCCTAACATCACTAAGATGGATAAGGCGTCCATTATTGGAGATGCTGTATTGTATGTGCAAGACCTGGAAAAGCAGGCTAAGAAGCTGAAAGCTGAGATTTTGGGACTTGAAGCATCCTTAGAAGGATCAGAGGAAAATCAAGGATCAACCCAAAATCCAACCAAGAATAAATTAGTTACAGACAATTACCATTTAATTCCCAAGGGAGCCATTCAGATAGATGTGTCTCAAGTAAAGGAGAAAGGCTTTCATGTGAAAGTGGGATGCAACAGAGGAGTAGGGGTGGCCACAGCACTTTACAAGGCTATAGAATCCCTTACAAGCTTCACTGTTCAGAGTTCAAACTTGAACATGGTTAATTCCAAGATATTCGAAATAACACTCGCTTTGAACGTAAAAGAGTGCGAGCACGAGATCATGAACTTACCAAATTTGAAACTTTGGGTGACTGGAGCTTTTCTGAACCAGGGTTTTGAATTAGCTACAGGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

328

Amino Acids

36.02

Weight (kDa)

4.97

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 143 - 187 6.6e-09 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 20
AciI CCGC 1 cut(s) 168
AclWI GGATC 4 cut(s) 128, 248, 625, 643
AcoI YGGCCR 1 cut(s) 777
AcsI RAATTY 1 cut(s) 922
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 1 cut(s) 274
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 6 cut(s) 602, 834, 841, 883, 928, 968
AjnI CCWGG 2 cut(s) 555, 957
AluBI AGCT 6 cut(s) 143, 577, 584, 816, 947, 974
AluI AGCT 6 cut(s) 143, 577, 584, 816, 947, 974
Alw21I GWGCWC 1 cut(s) 903
Alw26I GTCTC 1 cut(s) 723
AlwI GGATC 4 cut(s) 128, 248, 625, 643
Aor13HI TCCGGA 1 cut(s) 20
AoxI GGCC 1 cut(s) 777
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 1 cut(s) 922
AseI ATTAAT 1 cut(s) 54
AsuHPI GGTGA 1 cut(s) 949
AsuII TTCGAA 1 cut(s) 864
BalI TGGCCA 1 cut(s) 779
BauI CACGAG 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 903
BbvI GCAGC 1 cut(s) 152
BccI CCATC 3 cut(s) 4, 320, 502
BciT130I CCWGG 2 cut(s) 557, 959
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 723
BfmI CTRYAG 2 cut(s) 798, 975
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 2 cut(s) 557, 959
BmiI GGNNCC 2 cut(s) 492, 700
BmrFI CCNGG 2 cut(s) 557, 959
BmsI GCATC 5 cut(s) 26, 76, 523, 614, 746
BpmI CTGGAG 1 cut(s) 963
Bpu14I TTCGAA 1 cut(s) 864
BpuEI CTTGAG 1 cut(s) 705
Bsa29I ATCGAT 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 518
BsaJI CCNNGG 4 cut(s) 426, 486, 693, 958
BsaWI WCCGGW 1 cut(s) 20
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 759, 789
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 100, 946
Bse3DI GCAATG 1 cut(s) 476
BseAI TCCGGA 1 cut(s) 20
BseBI CCWGG 2 cut(s) 557, 959
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 4 cut(s) 426, 486, 693, 958
BseGI GGATG 6 cut(s) 10, 15, 17, 462, 605, 761
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 1 cut(s) 476
BseMII CTCAG 2 cut(s) 158, 576
BseNI ACTGG 2 cut(s) 100, 946
BseRI GAGGAG 1 cut(s) 780
BseXI GCAGC 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 1 cut(s) 779
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 419
BsiHKAI GWGCWC 1 cut(s) 903
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 2 cut(s) 358, 609
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 723
BsmFI GGGAC 2 cut(s) 358, 609
BsnI GGCC 1 cut(s) 779
Bsp119I TTCGAA 1 cut(s) 864
Bsp1286I GDGCHC 1 cut(s) 903
Bsp13I TCCGGA 1 cut(s) 20
Bsp143I GATC 9 cut(s) 31, 109, 133, 229, 253, 412, 617, 635, 906
BspACI CCGC 1 cut(s) 168
BspANI GGCC 1 cut(s) 779
BspCNI CTCAG 2 cut(s) 157, 577
BspDI ATCGAT 1 cut(s) 108
BspEI TCCGGA 1 cut(s) 20
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 2 cut(s) 492, 700
BspPI GGATC 4 cut(s) 128, 248, 625, 643
BspT104I TTCGAA 1 cut(s) 864
BsrDI GCAATG 1 cut(s) 476
BsrI ACTGG 2 cut(s) 100, 946
BssECI CCNNGG 4 cut(s) 426, 486, 693, 958
BssMI GATC 9 cut(s) 31, 109, 133, 229, 253, 412, 617, 635, 906
BssNI GRCGYC 1 cut(s) 518
BssSI CACGAG 1 cut(s) 902
BssT1I CCWWGG 3 cut(s) 426, 486, 693
Bst2BI CACGAG 1 cut(s) 902
Bst2UI CCWGG 2 cut(s) 557, 959
Bst4CI ACNGT 2 cut(s) 211, 823
BstACI GRCGYC 1 cut(s) 518
BstBI TTCGAA 1 cut(s) 864
BstC8I GCNNGC 2 cut(s) 567, 899
BstDEI CTNAG 7 cut(s) 35, 144, 269, 504, 570, 585, 610
BstF5I GGATG 6 cut(s) 10, 15, 17, 462, 605, 761
BstKTI GATC 9 cut(s) 34, 112, 136, 232, 256, 415, 620, 638, 909
BstMAI GTCTC 1 cut(s) 723
BstMBI GATC 9 cut(s) 31, 109, 133, 229, 253, 412, 617, 635, 906
BstMCI CGRYCG 1 cut(s) 419
BstNI CCWGG 2 cut(s) 557, 959
BstSCI CCNGG 2 cut(s) 555, 957
BstSFI CTRYAG 2 cut(s) 798, 975
BstV1I GCAGC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 1 cut(s) 779
BsuTUI ATCGAT 1 cut(s) 108
BtsCI GGATG 6 cut(s) 10, 15, 17, 462, 605, 761
BtsIMutI CAGTG 2 cut(s) 201, 819
Cac8I GCNNGC 2 cut(s) 567, 899
CciI TCATGA 1 cut(s) 909
ClaI ATCGAT 1 cut(s) 108
CseI GACGC 2 cut(s) 37, 507
Csp6I GTAC 1 cut(s) 273
CviAII CATG 4 cut(s) 29, 743, 844, 910
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 7 cut(s) 35, 144, 269, 504, 570, 585, 610
DpnI GATC 9 cut(s) 33, 111, 135, 231, 255, 414, 619, 637, 908
DpnII GATC 9 cut(s) 31, 109, 133, 229, 253, 412, 617, 635, 906
EaeI YGGCCR 1 cut(s) 777
Eco130I CCWWGG 3 cut(s) 426, 486, 693
EcoRII CCWGG 2 cut(s) 555, 957
EcoT14I CCWWGG 3 cut(s) 426, 486, 693
EcoT22I ATGCAT 1 cut(s) 478
ErhI CCWWGG 3 cut(s) 426, 486, 693
FaeI CATG 4 cut(s) 32, 746, 847, 913
FaqI GGGAC 2 cut(s) 358, 609
FatI CATG 4 cut(s) 28, 742, 843, 909
FauI CCCGC 1 cut(s) 161
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 6 cut(s) 4, 17, 22, 469, 592, 768
Fsp4HI GCNGC 1 cut(s) 141
GluI GCNGC 1 cut(s) 141
GsuI CTGGAG 1 cut(s) 963
HaeIII GGCC 1 cut(s) 779
HapII CCGG 1 cut(s) 21
HgaI GACGC 2 cut(s) 37, 507
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 4 cut(s) 32, 746, 847, 913
HindIII AAGCTT 1 cut(s) 814
HinfI GANTC 2 cut(s) 335, 803
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 949
Hpy188I TCNGA 5 cut(s) 436, 622, 708, 828, 954
Hpy188III TCNNGA 4 cut(s) 21, 155, 420, 910
Hpy99I CGWCG 1 cut(s) 397
HpyAV CCTTC 5 cut(s) 435, 441, 447, 454, 608
HpyCH4III ACNGT 2 cut(s) 211, 823
HpyCH4IV ACGT 1 cut(s) 885
HpyCH4V TGCA 5 cut(s) 17, 89, 476, 550, 759
HpyF3I CTNAG 7 cut(s) 35, 144, 269, 504, 570, 585, 610
HpySE526I ACGT 1 cut(s) 885
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 4 cut(s) 32, 746, 847, 913
Kpn2I TCCGGA 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 9 cut(s) 31, 109, 133, 229, 253, 412, 617, 635, 906
LmnI GCTCC 2 cut(s) 698, 944
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 5 cut(s) 26, 76, 523, 614, 746
MaeII ACGT 1 cut(s) 885
MaeIII GTNAC 4 cut(s) 41, 66, 667, 937
MalI GATC 9 cut(s) 33, 111, 135, 231, 255, 414, 619, 637, 908
MboI GATC 9 cut(s) 31, 109, 133, 229, 253, 412, 617, 635, 906
MboII GAAGA 4 cut(s) 143, 326, 329, 409
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 903
MlsI TGGCCA 1 cut(s) 779
MluCI AATT 6 cut(s) 662, 676, 687, 850, 922, 968
MluNI TGGCCA 1 cut(s) 779
MmeI TCCRAC 2 cut(s) 221, 674
MnlI CCTC 4 cut(s) 32, 270, 616, 758
Mox20I TGGCCA 1 cut(s) 779
Mph1103I ATGCAT 1 cut(s) 478
MroI TCCGGA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 779
MseI TTAA 4 cut(s) 54, 150, 686, 849
MslI CAYNNNNRTG 1 cut(s) 506
Msp20I TGGCCA 1 cut(s) 779
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 2 cut(s) 557, 959
MvaI CCWGG 2 cut(s) 557, 959
NdeII GATC 9 cut(s) 31, 109, 133, 229, 253, 412, 617, 635, 906
NlaIII CATG 4 cut(s) 32, 746, 847, 913
NlaIV GGNNCC 2 cut(s) 492, 700
NmuCI GTSAC 2 cut(s) 41, 937
NsiI ATGCAT 1 cut(s) 478
NspV TTCGAA 1 cut(s) 864
PagI TCATGA 1 cut(s) 909
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 882
PfeI GAWTC 2 cut(s) 335, 803
PkrI GCNGC 1 cut(s) 142
PshBI ATTAAT 1 cut(s) 54
Psp6I CCWGG 2 cut(s) 555, 957
PspGI CCWGG 2 cut(s) 555, 957
PspN4I GGNNCC 2 cut(s) 492, 700
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 506
SaqAI TTAA 4 cut(s) 54, 150, 686, 849
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 9 cut(s) 31, 109, 133, 229, 253, 412, 617, 635, 906
ScrFI CCNGG 2 cut(s) 557, 959
SduI GDGCHC 1 cut(s) 903
SfaNI GCATC 5 cut(s) 26, 76, 523, 614, 746
SfcI CTRYAG 2 cut(s) 798, 975
SfuI TTCGAA 1 cut(s) 864
SmiMI CAYNNNNRTG 1 cut(s) 506
SmlI CTYRAG 1 cut(s) 720
SmoI CTYRAG 1 cut(s) 720
Sse9I AATT 6 cut(s) 662, 676, 687, 850, 922, 968
SsiI CCGC 1 cut(s) 168
SspI AATATT 1 cut(s) 238
StyD4I CCNGG 2 cut(s) 555, 957
StyI CCWWGG 3 cut(s) 426, 486, 693
TaaI ACNGT 2 cut(s) 211, 823
TaiI ACGT 1 cut(s) 888
TaqI TCGA 4 cut(s) 96, 108, 419, 864
TaqII GACCGA 1 cut(s) 405
TasI AATT 6 cut(s) 662, 676, 687, 850, 922, 968
TatI WGTACW 1 cut(s) 272
TfiI GAWTC 2 cut(s) 335, 803
Tru1I TTAA 4 cut(s) 54, 150, 686, 849
Tru9I TTAA 4 cut(s) 54, 150, 686, 849
TscAI CASTG 2 cut(s) 208, 826
TseFI GTSAC 2 cut(s) 41, 937
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 2 cut(s) 41, 937
TspDTI ATGAA 7 cut(s) 67, 94, 144, 314, 473, 731, 926
TspRI CASTG 2 cut(s) 208, 826
VspI ATTAAT 1 cut(s) 54
XapI RAATTY 1 cut(s) 922
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.