RchiOBHm_Chr3g0482861

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0003463
Physical Location & Seq
Forward (+)
28954491 .. 28955778
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 867 bp
ATGCAAAGGGTATACGCACCAAAATATGATGGCAAGTATTTATACAAGCTTATCAAGGAAAAATTAGGAGACAGACGATTGAACCAGACATTGACCAATGTTGTGATCCCAACTTTTGATATAAGTTGCCTCCAACCAGCTATCTTCTCCACCTACGAGATCCGGCTCTACTGTCGGATATATGCATCGATCGGAACTTCAGCAGCACCAACTTATCTTCCCTCGCATTATTTCAAAACAGCAACAAGAGAATTCCATCTCGTTGATGGGGGTGTCGCTGCTAAGAACCCGGGTTTGACTGCTATTACCCAAGTGACAAAAGAAATCAAGAAGGGAAATTCTGACTTCTCTCCGGTTGAGGCAGCTGATCAGGGTAATAGGTTCCTCCTCATATCTTTGGGTACTGGTTCATCAGCAAAATCCAAGTACGATGCCAGAGTAGCAGCTAAATGGGGTATTATGCCATGGTTGATGCAGGCCAACTTTCAATGTCCATTGTTAGATGTACTCACTGAAGCAAGTAGTGACATGGTAGATTGCCACTTTTCTACCCTTTTCCAAGCTCTTTGGTCTGACGATAACTACCTTCGAATTCAGGATGACCAATTGAGCACCGACTTGAATTCCATGGATAATGCTACGCCCGAAAACTTGAAAAAACTTGTAAAAGTGGGAGAGGCACTGTTAAAGAAACAAGCTTCAAAGTTGAATATAGCTACCGGCCTCCATGAGCCTGATGAACGTCATATTACTAATGAAGAGGCTCTCAGAAGGTACTTATTGATTACAAATTACTCTCTATACGTACTGGGATTTGTATCAAAGCATCATAAACACATATTCATTAATAGTACTATTGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

288

Amino Acids

32.45

Weight (kDa)

8.7

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 12
AclWI GGATC 2 cut(s) 100, 154
AcsI RAATTY 4 cut(s) 251, 337, 591, 622
AcuI CTGAAG 2 cut(s) 183, 534
AfaI GTAC 6 cut(s) 403, 428, 507, 776, 807, 853
AgsI TTSAA 7 cut(s) 82, 235, 488, 622, 655, 702, 709
AluBI AGCT 7 cut(s) 49, 140, 365, 446, 563, 698, 716
AluI AGCT 7 cut(s) 49, 140, 365, 446, 563, 698, 716
Alw21I GWGCWC 1 cut(s) 614
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 2 cut(s) 100, 154
Ama87I CYCGRG 1 cut(s) 289
AoxI GGCC 2 cut(s) 477, 721
ApeKI GCWGC 4 cut(s) 203, 278, 362, 443
ApoI RAATTY 4 cut(s) 251, 337, 591, 622
AseI ATTAAT 1 cut(s) 846
AsuC2I CCSGG 2 cut(s) 290, 291
AsuII TTCGAA 1 cut(s) 589
AvaI CYCGRG 1 cut(s) 289
Bbv12I GWGCWC 1 cut(s) 614
BbvI GCAGC 4 cut(s) 215, 265, 374, 455
BccI CCATC 3 cut(s) 23, 260, 264
BclI TGATCA 1 cut(s) 367
BcnI CCSGG 2 cut(s) 290, 291
BcoDI GTCTC 1 cut(s) 63
BisI GCNGC 4 cut(s) 204, 279, 363, 444
BlsI GCNGC 4 cut(s) 205, 280, 364, 445
BmcAI AGTACT 1 cut(s) 853
Bme1390I CCNGG 2 cut(s) 290, 291
BmeT110I CYCGRG 1 cut(s) 289
BmiI GGNNCC 1 cut(s) 383
BmrFI CCNGG 2 cut(s) 290, 291
BmrI ACTGGG 1 cut(s) 818
BmsI GCATC 4 cut(s) 194, 421, 462, 835
BmuI ACTGGG 1 cut(s) 818
Bpu14I TTCGAA 1 cut(s) 589
BpuMI CCSGG 2 cut(s) 290, 291
Bsa29I ATCGAT 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 805
BsaBI GATNNNNATC 1 cut(s) 817
BsaJI CCNNGG 3 cut(s) 289, 464, 627
BsaWI WCCGGW 1 cut(s) 352
Bse118I RCCGGY 1 cut(s) 719
Bse1I ACTGG 2 cut(s) 409, 813
Bse8I GATNNNNATC 1 cut(s) 817
BseCI ATCGAT 1 cut(s) 188
BseDI CCNNGG 3 cut(s) 289, 464, 627
BseGI GGATG 1 cut(s) 604
BseJI GATNNNNATC 1 cut(s) 817
BseMII CTCAG 1 cut(s) 781
BseNI ACTGG 2 cut(s) 409, 813
BseRI GAGGAG 1 cut(s) 377
BseXI GCAGC 4 cut(s) 215, 265, 374, 455
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 2 cut(s) 479, 723
BshVI ATCGAT 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 289
BsiSI CCGG 4 cut(s) 163, 290, 353, 720
BsmAI GTCTC 1 cut(s) 63
BsnI GGCC 2 cut(s) 479, 723
BsoBI CYCGRG 1 cut(s) 289
Bsp119I TTCGAA 1 cut(s) 589
Bsp1286I GDGCHC 1 cut(s) 614
Bsp143I GATC 4 cut(s) 105, 159, 189, 367
Bsp19I CCATGG 2 cut(s) 464, 627
BspANI GGCC 2 cut(s) 479, 723
BspCNI CTCAG 1 cut(s) 780
BspDI ATCGAT 1 cut(s) 188
BspLI GGNNCC 1 cut(s) 383
BspPI GGATC 2 cut(s) 100, 154
BspT104I TTCGAA 1 cut(s) 589
BsrFI RCCGGY 1 cut(s) 719
BsrI ACTGG 2 cut(s) 409, 813
BssAI RCCGGY 1 cut(s) 719
BssECI CCNNGG 3 cut(s) 289, 464, 627
BssMI GATC 4 cut(s) 105, 159, 189, 367
BssNAI GTATAC 1 cut(s) 13
BssT1I CCWWGG 2 cut(s) 464, 627
Bst1107I GTATAC 1 cut(s) 13
Bst4CI ACNGT 2 cut(s) 173, 684
Bst6I CTCTTC 1 cut(s) 753
BstBAI YACGTR 1 cut(s) 805
BstBI TTCGAA 1 cut(s) 589
BstC8I GCNNGC 1 cut(s) 477
BstDEI CTNAG 2 cut(s) 282, 767
BstDSI CCRYGG 2 cut(s) 464, 627
BstF5I GGATG 1 cut(s) 604
BstKTI GATC 4 cut(s) 108, 162, 192, 370
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 4 cut(s) 105, 159, 189, 367
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstSCI CCNGG 2 cut(s) 288, 289
BstSNI TACGTA 1 cut(s) 805
BstV1I GCAGC 4 cut(s) 215, 265, 374, 455
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BstZ17I GTATAC 1 cut(s) 13
Bsu15I ATCGAT 1 cut(s) 188
BsuRI GGCC 2 cut(s) 479, 723
BsuTUI ATCGAT 1 cut(s) 188
BtgI CCRYGG 2 cut(s) 464, 627
BtsCI GGATG 1 cut(s) 604
BtsIMutI CAGTG 2 cut(s) 510, 680
Cac8I GCNNGC 1 cut(s) 477
Cfr10I RCCGGY 1 cut(s) 719
Cfr9I CCCGGG 1 cut(s) 289
ClaI ATCGAT 1 cut(s) 188
Csp6I GTAC 6 cut(s) 402, 427, 506, 775, 806, 852
CviAII CATG 4 cut(s) 465, 529, 628, 728
CviQI GTAC 6 cut(s) 402, 427, 506, 775, 806, 852
DdeI CTNAG 2 cut(s) 282, 767
DpnI GATC 4 cut(s) 107, 161, 191, 369
DpnII GATC 4 cut(s) 105, 159, 189, 367
Eam1104I CTCTTC 1 cut(s) 753
EarI CTCTTC 1 cut(s) 753
Eco105I TACGTA 1 cut(s) 805
Eco130I CCWWGG 2 cut(s) 464, 627
Eco57I CTGAAG 2 cut(s) 183, 534
Eco88I CYCGRG 1 cut(s) 289
EcoRI GAATTC 3 cut(s) 251, 591, 622
EcoT14I CCWWGG 2 cut(s) 464, 627
EcoT22I ATGCAT 1 cut(s) 187
ErhI CCWWGG 2 cut(s) 464, 627
FaeI CATG 4 cut(s) 468, 532, 631, 731
FatI CATG 4 cut(s) 464, 528, 627, 727
FbaI TGATCA 1 cut(s) 367
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 4 cut(s) 204, 279, 363, 444
FokI GGATG 1 cut(s) 611
Fsp4HI GCNGC 4 cut(s) 204, 279, 363, 444
GluI GCNGC 4 cut(s) 204, 279, 363, 444
HaeIII GGCC 2 cut(s) 479, 723
HapII CCGG 4 cut(s) 163, 290, 353, 720
Hin1II CATG 4 cut(s) 468, 532, 631, 731
HindIII AAGCTT 2 cut(s) 47, 696
HpaII CCGG 4 cut(s) 163, 290, 353, 720
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 5 cut(s) 177, 194, 343, 574, 770
Hpy188III TCNNGA 2 cut(s) 328, 596
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 325, 596, 765
HpyCH4III ACNGT 2 cut(s) 173, 684
HpyCH4IV ACGT 2 cut(s) 742, 804
HpyCH4V TGCA 3 cut(s) 4, 185, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpyF3I CTNAG 2 cut(s) 282, 767
HpySE526I ACGT 2 cut(s) 742, 804
Hsp92II CATG 4 cut(s) 468, 532, 631, 731
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 4 cut(s) 105, 159, 189, 367
Lsp1109I GCAGC 4 cut(s) 215, 265, 374, 455
LweI GCATC 4 cut(s) 194, 421, 462, 835
MaeII ACGT 2 cut(s) 742, 804
MaeIII GTNAC 2 cut(s) 313, 524
MalI GATC 4 cut(s) 107, 161, 191, 369
MboI GATC 4 cut(s) 105, 159, 189, 367
MboII GAAGA 3 cut(s) 136, 209, 770
MfeI CAATTG 1 cut(s) 605
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 1 cut(s) 614
MluCI AATT 8 cut(s) 62, 251, 337, 591, 605, 622, 790, 862
MmeI TCCRAC 2 cut(s) 155, 157
MnlI CCTC 8 cut(s) 140, 232, 352, 395, 398, 670, 734, 754
Mph1103I ATGCAT 1 cut(s) 187
MseI TTAA 3 cut(s) 686, 846, 861
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 4 cut(s) 163, 290, 353, 720
MspR9I CCNGG 2 cut(s) 290, 291
MunI CAATTG 1 cut(s) 605
MwoI GCNNNNNNNGC 1 cut(s) 440
NciI CCSGG 2 cut(s) 290, 291
NcoI CCATGG 2 cut(s) 464, 627
NdeII GATC 4 cut(s) 105, 159, 189, 367
NlaIII CATG 4 cut(s) 468, 532, 631, 731
NlaIV GGNNCC 1 cut(s) 383
NmuCI GTSAC 2 cut(s) 313, 524
NsiI ATGCAT 1 cut(s) 187
NspV TTCGAA 1 cut(s) 589
PkrI GCNGC 4 cut(s) 205, 280, 364, 445
Ple19I CGATCG 1 cut(s) 192
Ppu21I YACGTR 1 cut(s) 805
PshBI ATTAAT 1 cut(s) 846
PspN4I GGNNCC 1 cut(s) 383
PsuI RGATCY 1 cut(s) 159
PvuI CGATCG 1 cut(s) 192
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 6 cut(s) 403, 428, 507, 776, 807, 853
RsaNI GTAC 6 cut(s) 402, 427, 506, 775, 806, 852
SaqAI TTAA 3 cut(s) 686, 846, 861
SatI GCNGC 4 cut(s) 204, 279, 363, 444
Sau3AI GATC 4 cut(s) 105, 159, 189, 367
ScaI AGTACT 1 cut(s) 853
ScrFI CCNGG 2 cut(s) 290, 291
SduI GDGCHC 1 cut(s) 614
SfaNI GCATC 4 cut(s) 194, 421, 462, 835
SfuI TTCGAA 1 cut(s) 589
SmaI CCCGGG 1 cut(s) 291
SnaBI TACGTA 1 cut(s) 805
Sse9I AATT 8 cut(s) 62, 251, 337, 591, 605, 622, 790, 862
StyD4I CCNGG 2 cut(s) 288, 289
StyI CCWWGG 2 cut(s) 464, 627
TaaI ACNGT 2 cut(s) 173, 684
TaiI ACGT 2 cut(s) 745, 807
TaqI TCGA 2 cut(s) 188, 589
TasI AATT 8 cut(s) 62, 251, 337, 591, 605, 622, 790, 862
TatI WGTACW 2 cut(s) 505, 851
Tru1I TTAA 3 cut(s) 686, 846, 861
Tru9I TTAA 3 cut(s) 686, 846, 861
TscAI CASTG 2 cut(s) 517, 687
TseFI GTSAC 2 cut(s) 313, 524
TseI GCWGC 4 cut(s) 203, 278, 362, 443
Tsp45I GTSAC 2 cut(s) 313, 524
TspDTI ATGAA 4 cut(s) 399, 753, 771, 832
TspMI CCCGGG 1 cut(s) 289
TspRI CASTG 2 cut(s) 517, 687
VspI ATTAAT 1 cut(s) 846
XapI RAATTY 4 cut(s) 251, 337, 591, 622
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XmaI CCCGGG 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 12
ZrmI AGTACT 1 cut(s) 853
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.