RchiOBHm_Chr2g0092811

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008176
Physical Location & Seq
Reverse (-)
6207990 .. 6211098
3109 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1005 bp
ATGGCTTTTGCCCCTGACCCGGGCTATCAGGCCCTCAGCAAAGGCTTGGACATTATACAACAGCCATCTGAAGCCGAAGCCGGGCATCAGGAGTCTTTGGAGAAACGTATTGCTGAAGAATGGATGCCTTCTTCACGGAACATGACATATATGCTTGCGCAGTTCAAGCAGAAGGTTTCTATGCTTGATAACTCTGGGAAGCCAGTCCTTGCATCAACTGACAGTTCAAACCCCTGGTGCGCCCTGCTAGAAGAAGTGGTCAGTAAAGCTTACGGAACACTTGGTAAGCCTGAGATTTGTCCTGCTTCAACAGATGCTCACTATTTCCAGCTACAAGGCTTGCCAGCAATTGGATTCTCTCCTATGGCCAACACTCATTTTCTGCTCCATGACCATAACGAGGTCGTGGCTGTTGGAAGATTCCAAAATGGTGGTGCATTCAACGTTATTCCAGATTCTGCTGAACAAAGGGTTGAAGAGGTTGAACATGTCGTAAGGGAAGAGAAGTTGAGGGAAGCATATGAGCTTATCGAGATATATTGCGAACTTATTGCAGCACGATTGCCAATGTTTGAGTCGCAGAAGAACTGCCTAATTGATCTGGAGGAAGCAGTATCAAGTGTAATTTTTGCGAGGGGACCTACTAATGTCCAAGTTCCTCCTAATAGTAGCATGGCCAATAAAGCTCATGTGGAAGCTCCCCCTGGTCCGCTTCCACCTGATAAGCCTCCTAATGCTGAAGTTCCACGCAGAAATTATGAGTTTCAGGATGGACCCGTGAACTCTAATGAGCAGATTGCAAGATCATCACCTCGCTTGGAGGATTCTGCTTCTACAAATGTTTCTGCCAACAAAGCAACCACATCTACCACATTTCATCCAGAAACGATATCCTCAGGAAGTGATCGAATGGAAATGCATGGGGATGATAATTCTTCGGGCAGGCAGAACTGGAATATGCAATTTAAGGGTACTGCCTCTGCAGCACAGGCAGCTGCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

334

Amino Acids

36.54

Weight (kDa)

4.93

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 154 - 212 2.1e-15 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 159
AccB7I CCANNNNNTGG 1 cut(s) 350
AciI CCGC 1 cut(s) 710
AclI AACGTT 1 cut(s) 444
AcoI YGGCCR 2 cut(s) 366, 675
AcuI CTGAAG 3 cut(s) 90, 135, 759
AfaI GTAC 1 cut(s) 973
AfiI CCNNNNNNNGG 5 cut(s) 19, 20, 81, 350, 400
AflIII ACRYGT 1 cut(s) 487
AgsI TTSAA 6 cut(s) 166, 228, 309, 442, 476, 485
AjnI CCWGG 2 cut(s) 233, 703
AluBI AGCT 6 cut(s) 269, 331, 526, 686, 698, 995
AluI AGCT 6 cut(s) 269, 331, 526, 686, 698, 995
AlwNI CAGNNNCTG 2 cut(s) 458, 998
Ama87I CYCGRG 1 cut(s) 19
AoxI GGCC 3 cut(s) 30, 366, 675
ApeKI GCWGC 4 cut(s) 554, 983, 992, 995
AspLEI GCGC 2 cut(s) 160, 242
AspS9I GGNCC 4 cut(s) 31, 638, 707, 773
AsuC2I CCSGG 3 cut(s) 20, 21, 82
AsuHPI GGTGA 1 cut(s) 801
AvaI CYCGRG 1 cut(s) 19
AvaII GGWCC 3 cut(s) 638, 707, 773
AxyI CCTNAGG 1 cut(s) 895
BalI TGGCCA 2 cut(s) 368, 677
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 566, 982, 995
BccI CCATC 2 cut(s) 73, 764
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 2 cut(s) 235, 705
BcnI CCSGG 3 cut(s) 20, 21, 82
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 981
BisI GCNGC 4 cut(s) 555, 984, 993, 996
BlsI GCNGC 4 cut(s) 556, 985, 994, 997
Bme1390I CCNGG 5 cut(s) 20, 21, 82, 235, 705
Bme18I GGWCC 3 cut(s) 638, 707, 773
BmeT110I CYCGRG 1 cut(s) 19
BmgT120I GGNCC 4 cut(s) 31, 638, 707, 773
BmiI GGNNCC 2 cut(s) 639, 775
BmrFI CCNGG 5 cut(s) 20, 21, 82, 235, 705
BmsI GCATC 4 cut(s) 94, 114, 221, 304
BpmI CTGGAG 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 35
BpuMI CCSGG 3 cut(s) 20, 21, 82
BsaJI CCNNGG 3 cut(s) 19, 233, 703
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 20, 81, 350, 400
Bse1I ACTGG 2 cut(s) 203, 956
Bse21I CCTNAGG 1 cut(s) 895
BseBI CCWGG 2 cut(s) 235, 705
BseDI CCNNGG 3 cut(s) 19, 233, 703
BseGI GGATG 4 cut(s) 129, 775, 877, 931
BseLI CCNNNNNNNGG 5 cut(s) 19, 20, 81, 350, 400
BseMII CTCAG 3 cut(s) 49, 282, 909
BseNI ACTGG 2 cut(s) 203, 956
BseXI GCAGC 3 cut(s) 566, 982, 995
BshFI GGCC 3 cut(s) 32, 368, 677
BsiHKCI CYCGRG 1 cut(s) 19
BsiSI CCGG 2 cut(s) 20, 81
BslFI GGGAC 1 cut(s) 651
BslI CCNNNNNNNGG 5 cut(s) 19, 20, 81, 350, 400
BsmFI GGGAC 1 cut(s) 651
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 3 cut(s) 32, 368, 677
BsoBI CYCGRG 1 cut(s) 19
Bsp143I GATC 3 cut(s) 598, 803, 904
BspACI CCGC 1 cut(s) 710
BspANI GGCC 3 cut(s) 32, 368, 677
BspCNI CTCAG 3 cut(s) 48, 283, 908
BspLI GGNNCC 2 cut(s) 639, 775
BspMAI CTGCAG 1 cut(s) 985
BsrI ACTGG 2 cut(s) 203, 956
BssECI CCNNGG 3 cut(s) 19, 233, 703
BssMI GATC 3 cut(s) 598, 803, 904
Bst2UI CCWGG 2 cut(s) 235, 705
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 2 cut(s) 471, 495
BstC8I GCNNGC 4 cut(s) 156, 341, 345, 944
BstDEI CTNAG 3 cut(s) 35, 291, 895
BstF5I GGATG 4 cut(s) 129, 775, 877, 931
BstHHI GCGC 2 cut(s) 160, 242
BstKTI GATC 3 cut(s) 601, 806, 907
BstMBI GATC 3 cut(s) 598, 803, 904
BstMWI GCNNNNNNNGC 6 cut(s) 166, 683, 854, 983, 989, 992
BstNI CCWGG 2 cut(s) 235, 705
BstNSI RCATGY 1 cut(s) 491
BstSCI CCNGG 5 cut(s) 18, 19, 80, 233, 703
BstSFI CTRYAG 1 cut(s) 981
BstV1I GCAGC 3 cut(s) 566, 982, 995
BstXI CCANNNNNNTGG 1 cut(s) 431
Bsu36I CCTNAGG 1 cut(s) 895
BsuRI GGCC 3 cut(s) 32, 368, 677
BtsCI GGATG 4 cut(s) 129, 775, 877, 931
Cac8I GCNNGC 4 cut(s) 156, 341, 345, 944
CaiI CAGNNNCTG 2 cut(s) 458, 998
CfoI GCGC 2 cut(s) 160, 242
Cfr13I GGNCC 4 cut(s) 31, 638, 707, 773
Cfr9I CCCGGG 1 cut(s) 19
Csp6I GTAC 1 cut(s) 972
CviAII CATG 6 cut(s) 142, 389, 488, 673, 689, 920
CviQI GTAC 1 cut(s) 972
DdeI CTNAG 3 cut(s) 35, 291, 895
DpnI GATC 3 cut(s) 600, 805, 906
DpnII GATC 3 cut(s) 598, 803, 904
EaeI YGGCCR 2 cut(s) 366, 675
Eam1104I CTCTTC 2 cut(s) 471, 495
EarI CTCTTC 2 cut(s) 471, 495
Eco32I GATATC 1 cut(s) 891
Eco47I GGWCC 3 cut(s) 638, 707, 773
Eco57I CTGAAG 3 cut(s) 90, 135, 759
Eco81I CCTNAGG 1 cut(s) 895
Eco88I CYCGRG 1 cut(s) 19
EcoO109I RGGNCCY 2 cut(s) 31, 638
EcoRII CCWGG 2 cut(s) 233, 703
EcoRV GATATC 1 cut(s) 891
EcoT22I ATGCAT 1 cut(s) 921
FaeI CATG 6 cut(s) 145, 392, 491, 676, 692, 923
FaqI GGGAC 1 cut(s) 651
FatI CATG 6 cut(s) 141, 388, 487, 672, 688, 919
FauNDI CATATG 1 cut(s) 520
Fnu4HI GCNGC 4 cut(s) 555, 984, 993, 996
FokI GGATG 4 cut(s) 136, 782, 864, 938
Fsp4HI GCNGC 4 cut(s) 555, 984, 993, 996
FspBI CTAG 1 cut(s) 248
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 2 cut(s) 159, 241
GluI GCNGC 4 cut(s) 555, 984, 993, 996
GsuI CTGGAG 1 cut(s) 623
HaeIII GGCC 3 cut(s) 32, 368, 677
HapII CCGG 2 cut(s) 20, 81
HhaI GCGC 2 cut(s) 160, 242
Hin1II CATG 6 cut(s) 145, 392, 491, 676, 692, 923
Hin6I GCGC 2 cut(s) 158, 240
HinP1I GCGC 2 cut(s) 158, 240
HindIII AAGCTT 1 cut(s) 267
HinfI GANTC 6 cut(s) 92, 354, 420, 455, 575, 824
HpaII CCGG 2 cut(s) 20, 81
HphI GGTGA 1 cut(s) 801
Hpy166II GTNNAC 1 cut(s) 781
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 7 cut(s) 89, 452, 532, 602, 767, 881, 897
Hpy8I GTNNAC 1 cut(s) 781
HpyAV CCTTC 2 cut(s) 138, 166
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 2 cut(s) 106, 444
HpyCH4V TGCA 7 cut(s) 212, 437, 554, 800, 919, 961, 983
HpyF10VI GCNNNNNNNGC 6 cut(s) 166, 683, 854, 983, 989, 992
HpyF3I CTNAG 3 cut(s) 35, 291, 895
HpySE526I ACGT 2 cut(s) 106, 444
Hsp92II CATG 6 cut(s) 145, 392, 491, 676, 692, 923
HspAI GCGC 2 cut(s) 158, 240
Kzo9I GATC 3 cut(s) 598, 803, 904
LmnI GCTCC 2 cut(s) 390, 703
Lsp1109I GCAGC 3 cut(s) 566, 982, 995
LweI GCATC 4 cut(s) 94, 114, 221, 304
MaeI CTAG 1 cut(s) 248
MaeII ACGT 2 cut(s) 106, 444
MalI GATC 3 cut(s) 600, 805, 906
MboI GATC 3 cut(s) 598, 803, 904
MboII GAAGA 8 cut(s) 123, 128, 263, 429, 488, 512, 595, 927
MfeI CAATTG 1 cut(s) 348
MlsI TGGCCA 2 cut(s) 368, 677
MluCI AATT 6 cut(s) 348, 594, 624, 754, 931, 962
MluNI TGGCCA 2 cut(s) 368, 677
MlyI GAGTC 2 cut(s) 101, 584
MmeI TCCRAC 1 cut(s) 394
Mox20I TGGCCA 2 cut(s) 368, 677
Mph1103I ATGCAT 1 cut(s) 921
MscI TGGCCA 2 cut(s) 368, 677
MseI TTAA 1 cut(s) 966
MslI CAYNNNNRTG 1 cut(s) 924
Msp20I TGGCCA 2 cut(s) 368, 677
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 2 cut(s) 20, 81
MspR9I CCNGG 5 cut(s) 20, 21, 82, 235, 705
MunI CAATTG 1 cut(s) 348
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 2 cut(s) 235, 705
MwoI GCNNNNNNNGC 6 cut(s) 166, 683, 854, 983, 989, 992
NciI CCSGG 3 cut(s) 20, 21, 82
NdeI CATATG 1 cut(s) 520
NdeII GATC 3 cut(s) 598, 803, 904
NlaIII CATG 6 cut(s) 145, 392, 491, 676, 692, 923
NlaIV GGNNCC 2 cut(s) 639, 775
NsbI TGCGCA 1 cut(s) 159
NsiI ATGCAT 1 cut(s) 921
NspI RCATGY 1 cut(s) 491
PciI ACATGT 1 cut(s) 487
PctI GAATGC 1 cut(s) 437
PfeI GAWTC 4 cut(s) 354, 420, 455, 824
PflMI CCANNNNNTGG 1 cut(s) 350
PkrI GCNGC 4 cut(s) 556, 985, 994, 997
PleI GAGTC 2 cut(s) 100, 583
PpsI GAGTC 2 cut(s) 100, 583
PpuMI RGGWCCY 1 cut(s) 638
PscI ACATGT 1 cut(s) 487
Psp1406I AACGTT 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 2 cut(s) 233, 703
PspGI CCWGG 2 cut(s) 233, 703
PspN4I GGNNCC 2 cut(s) 639, 775
PspPI GGNCC 4 cut(s) 31, 638, 707, 773
PspPPI RGGWCCY 1 cut(s) 638
PstI CTGCAG 1 cut(s) 985
PstNI CAGNNNCTG 2 cut(s) 458, 998
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 1 cut(s) 973
RsaNI GTAC 1 cut(s) 972
RseI CAYNNNNRTG 1 cut(s) 924
SaqAI TTAA 1 cut(s) 966
SatI GCNGC 4 cut(s) 555, 984, 993, 996
Sau3AI GATC 3 cut(s) 598, 803, 904
Sau96I GGNCC 4 cut(s) 31, 638, 707, 773
SchI GAGTC 2 cut(s) 101, 584
ScrFI CCNGG 5 cut(s) 20, 21, 82, 235, 705
SfaNI GCATC 4 cut(s) 94, 114, 221, 304
SfcI CTRYAG 1 cut(s) 981
SinI GGWCC 3 cut(s) 638, 707, 773
SmaI CCCGGG 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 924
Sse9I AATT 6 cut(s) 348, 594, 624, 754, 931, 962
SsiI CCGC 1 cut(s) 710
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 5 cut(s) 18, 19, 80, 233, 703
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 2 cut(s) 109, 447
TaqI TCGA 2 cut(s) 531, 907
TasI AATT 6 cut(s) 348, 594, 624, 754, 931, 962
TfiI GAWTC 4 cut(s) 354, 420, 455, 824
Tru1I TTAA 1 cut(s) 966
Tru9I TTAA 1 cut(s) 966
TseI GCWGC 4 cut(s) 554, 983, 992, 995
TspDTI ATGAA 1 cut(s) 866
TspGWI ACGGA 2 cut(s) 151, 288
TspMI CCCGGG 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 350
VpaK11BI GGWCC 3 cut(s) 638, 707, 773
XceI RCATGY 1 cut(s) 491
XmaI CCCGGG 1 cut(s) 19
XspI CTAG 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.