RchiOBHm_Chr6g0257341

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008176
Physical Location & Seq
Forward (+)
12653556 .. 12654662
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23083

Sequence Viewer

Length: 789 bp
ATGACTTATTATCTGGTCCTAATTATGGTATGGAGTTTGGAGCCTAATCTTGTCTTTAGCTTGATTCATGTTGGTTTGTTTAATGTTCTTTACAGAAACTGCCCAATTGATCTGAAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCCGACGTACCAGAACTCATGGATATCCGCAAGCACTTCACAGAAAAATACCGAAAGGAGTTCGTCTCTGTAGCTGTTGAACTGCGGCCAGATAGTGGTGTAAGCCGCATGTTAATAGAGAAATTATCTACCAAATCACCTGATGGCCCTACCAAGATGAAAATTTTGGCTGCAATTGCAGAACATAATGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTCCTGAGGACCTGCTGAATGGACCAAATACGTTTGGAATGTCCAGTATGGATTCTGCTCCACCTCCACCTACTCATGATGTCAGGGGACCTCCTAATGTCCAAGTTCCGCCTAATAGCATGGCCAGTAAAGCTCATGTGGAACCTCCCTCTGGTCCACCTCTACCTGATCAACCTCCAATTGCTCAAGTTCCACGCAGAAATTATGAGTATCACGATGGACCTATGAATTCTAATGAGCAGAATGCAAGATCATCACGTCACTTGGAGGATTCTGCTTCTACAAATGTTTCTGCCAACAAAGCAATCACATCTGCCAAATTTCATCCAGAAACGACATCTTCAGGTACATCAATTTGTCACATAAAGATTGTGGTTATTGAATTCAACTATTTTGTGGTTGTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

262

Amino Acids

29.12

Weight (kDa)

5.99

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 31 - 114 2.5e-24 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 399
AccB7I CCANNNNNTGG 1 cut(s) 251
AciI CCGC 4 cut(s) 184, 241, 262, 488
AclWI GGATC 2 cut(s) 350, 363
AcoI YGGCCR 2 cut(s) 242, 501
AcsI RAATTY 4 cut(s) 318, 607, 698, 761
AcuI CTGAAG 2 cut(s) 134, 705
AfaI GTAC 2 cut(s) 165, 727
AfiI CCNNNNNNNGG 3 cut(s) 25, 251, 530
AgsI TTSAA 4 cut(s) 236, 371, 761, 766
AjiI CACGTC 1 cut(s) 638
AluBI AGCT 3 cut(s) 60, 230, 512
AluI AGCT 3 cut(s) 60, 230, 512
Alw26I GTCTC 1 cut(s) 226
AlwI GGATC 2 cut(s) 350, 363
AlwNI CAGNNNCTG 1 cut(s) 99
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 3 cut(s) 242, 301, 501
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 4 cut(s) 318, 607, 698, 761
AspS9I GGNCC 7 cut(s) 16, 302, 388, 401, 467, 533, 599
AsuHPI GGTGA 1 cut(s) 285
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 6 cut(s) 16, 388, 401, 467, 533, 599
AxyI CCTNAGG 1 cut(s) 384
BalI TGGCCA 1 cut(s) 503
BamHI GGATCC 1 cut(s) 355
BbvI GCAGC 1 cut(s) 313
BccI CCATC 2 cut(s) 293, 590
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 1 cut(s) 226
BfaI CTAG 1 cut(s) 150
BfmI CTRYAG 1 cut(s) 225
BfuAI ACCTGC 1 cut(s) 399
BisI GCNGC 3 cut(s) 242, 262, 327
BlsI GCNGC 3 cut(s) 243, 263, 328
Bme18I GGWCC 6 cut(s) 16, 388, 401, 467, 533, 599
BmeT110I CYCGRG 1 cut(s) 359
BmgBI CACGTC 1 cut(s) 638
BmgT120I GGNCC 7 cut(s) 16, 302, 388, 401, 467, 533, 599
BmiI GGNNCC 4 cut(s) 42, 357, 468, 522
BmsI GCATC 1 cut(s) 152
BplI GAGNNNNNCTC 2 cut(s) 206, 238
BpuEI CTTGAG 1 cut(s) 549
BsaJI CCNNGG 1 cut(s) 360
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 251, 530
Bse1I ACTGG 2 cut(s) 423, 504
Bse21I CCTNAGG 1 cut(s) 384
BseDI CCNNGG 1 cut(s) 360
BseGI GGATG 1 cut(s) 703
BseLI CCNNNNNNNGG 3 cut(s) 25, 251, 530
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 2 cut(s) 423, 504
BseXI GCAGC 1 cut(s) 313
BshFI GGCC 3 cut(s) 244, 303, 503
BsiHKCI CYCGRG 1 cut(s) 359
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 3 cut(s) 25, 251, 530
BsmAI GTCTC 1 cut(s) 226
BsmBI CGTCTC 1 cut(s) 226
BsmFI GGGAC 1 cut(s) 480
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 3 cut(s) 244, 303, 503
BsoBI CYCGRG 1 cut(s) 359
Bsp143I GATC 4 cut(s) 109, 355, 547, 629
BspACI CCGC 4 cut(s) 184, 241, 262, 488
BspANI GGCC 3 cut(s) 244, 303, 503
BspCNI CTCAG 1 cut(s) 376
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 4 cut(s) 42, 357, 468, 522
BspMI ACCTGC 1 cut(s) 399
BspPI GGATC 2 cut(s) 350, 363
BsrI ACTGG 2 cut(s) 423, 504
BssECI CCNNGG 1 cut(s) 360
BssMI GATC 4 cut(s) 109, 355, 547, 629
Bst6I CTCTTC 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 188
BstDEI CTNAG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 703
BstKTI GATC 4 cut(s) 112, 358, 550, 632
BstMAI GTCTC 1 cut(s) 226
BstMBI GATC 4 cut(s) 109, 355, 547, 629
BstMWI GCNNNNNNNGC 3 cut(s) 332, 509, 680
BstNSI RCATGY 1 cut(s) 268
BstSFI CTRYAG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 313
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 3 cut(s) 244, 303, 503
BtrI CACGTC 1 cut(s) 638
BtsCI GGATG 1 cut(s) 703
BveI ACCTGC 1 cut(s) 399
Cac8I GCNNGC 1 cut(s) 188
CaiI CAGNNNCTG 1 cut(s) 99
CciI TCATGA 1 cut(s) 454
Cfr13I GGNCC 7 cut(s) 16, 302, 388, 401, 467, 533, 599
Csp6I GTAC 2 cut(s) 164, 726
CviAII CATG 6 cut(s) 68, 175, 265, 455, 499, 515
CviQI GTAC 2 cut(s) 164, 726
DdeI CTNAG 1 cut(s) 384
DpnI GATC 4 cut(s) 111, 357, 549, 631
DpnII GATC 4 cut(s) 109, 355, 547, 629
EaeI YGGCCR 2 cut(s) 242, 501
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
EciI GGCGGA 1 cut(s) 477
Eco32I GATATC 1 cut(s) 181
Eco47I GGWCC 6 cut(s) 16, 388, 401, 467, 533, 599
Eco57I CTGAAG 2 cut(s) 134, 705
Eco81I CCTNAGG 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 359
EcoO109I RGGNCCY 2 cut(s) 388, 467
EcoRI GAATTC 2 cut(s) 607, 761
EcoRV GATATC 1 cut(s) 181
Esp3I CGTCTC 1 cut(s) 226
FaeI CATG 6 cut(s) 71, 178, 268, 458, 502, 518
FaqI GGGAC 1 cut(s) 480
FatI CATG 6 cut(s) 67, 174, 264, 454, 498, 514
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 242, 262, 327
FokI GGATG 1 cut(s) 690
Fsp4HI GCNGC 3 cut(s) 242, 262, 327
FspBI CTAG 1 cut(s) 150
GluI GCNGC 3 cut(s) 242, 262, 327
HaeIII GGCC 3 cut(s) 244, 303, 503
Hin1II CATG 6 cut(s) 71, 178, 268, 458, 502, 518
HinfI GANTC 3 cut(s) 64, 431, 650
HphI GGTGA 1 cut(s) 285
Hpy166II GTNNAC 1 cut(s) 536
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 5 cut(s) 359, 383, 455, 593, 707
Hpy8I GTNNAC 1 cut(s) 536
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 109
HpyCH4IV ACGT 3 cut(s) 162, 410, 637
HpyCH4V TGCA 4 cut(s) 143, 329, 335, 626
HpyF10VI GCNNNNNNNGC 3 cut(s) 332, 509, 680
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 3 cut(s) 162, 410, 637
Hsp92II CATG 6 cut(s) 71, 178, 268, 458, 502, 518
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 4 cut(s) 109, 355, 547, 629
LmnI GCTCC 2 cut(s) 40, 442
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 1 cut(s) 152
MaeI CTAG 1 cut(s) 150
MaeII ACGT 3 cut(s) 162, 410, 637
MaeIII GTNAC 2 cut(s) 638, 737
MalI GATC 4 cut(s) 111, 357, 549, 631
MboI GATC 4 cut(s) 109, 355, 547, 629
MboII GAAGA 2 cut(s) 383, 711
MfeI CAATTG 3 cut(s) 105, 330, 558
MflI RGATCY 1 cut(s) 355
MlsI TGGCCA 1 cut(s) 503
MluNI TGGCCA 1 cut(s) 503
Mox20I TGGCCA 1 cut(s) 503
MscI TGGCCA 1 cut(s) 503
MseI TTAA 2 cut(s) 81, 269
Msp20I TGGCCA 1 cut(s) 503
MunI CAATTG 3 cut(s) 105, 330, 558
Mva1269I GAATGC 1 cut(s) 628
MwoI GCNNNNNNNGC 3 cut(s) 332, 509, 680
NdeII GATC 4 cut(s) 109, 355, 547, 629
NlaIII CATG 6 cut(s) 71, 178, 268, 458, 502, 518
NlaIV GGNNCC 4 cut(s) 42, 357, 468, 522
NmuCI GTSAC 2 cut(s) 638, 737
NspI RCATGY 1 cut(s) 268
PagI TCATGA 1 cut(s) 454
PctI GAATGC 1 cut(s) 628
PfeI GAWTC 3 cut(s) 64, 431, 650
PflMI CCANNNNNTGG 1 cut(s) 251
PkrI GCNGC 3 cut(s) 243, 263, 328
PpuMI RGGWCCY 2 cut(s) 388, 467
Psp5II RGGWCCY 2 cut(s) 388, 467
PspN4I GGNNCC 4 cut(s) 42, 357, 468, 522
PspPI GGNCC 7 cut(s) 16, 302, 388, 401, 467, 533, 599
PspPPI RGGWCCY 2 cut(s) 388, 467
PstNI CAGNNNCTG 1 cut(s) 99
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 2 cut(s) 165, 727
RsaNI GTAC 2 cut(s) 164, 726
SaqAI TTAA 2 cut(s) 81, 269
SatI GCNGC 3 cut(s) 242, 262, 327
Sau3AI GATC 4 cut(s) 109, 355, 547, 629
Sau96I GGNCC 7 cut(s) 16, 302, 388, 401, 467, 533, 599
SfaNI GCATC 1 cut(s) 152
SfcI CTRYAG 1 cut(s) 225
SinI GGWCC 6 cut(s) 16, 388, 401, 467, 533, 599
SmlI CTYRAG 1 cut(s) 564
SmoI CTYRAG 1 cut(s) 564
SsiI CCGC 4 cut(s) 184, 241, 262, 488
SspMI CTAG 1 cut(s) 150
TaiI ACGT 3 cut(s) 165, 413, 640
TauI GCSGC 2 cut(s) 244, 264
TfiI GAWTC 3 cut(s) 64, 431, 650
Tru1I TTAA 2 cut(s) 81, 269
Tru9I TTAA 2 cut(s) 81, 269
TseFI GTSAC 2 cut(s) 638, 737
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 2 cut(s) 638, 737
TspDTI ATGAA 4 cut(s) 56, 329, 620, 692
Van91I CCANNNNNTGG 1 cut(s) 251
VpaK11BI GGWCC 6 cut(s) 16, 388, 401, 467, 533, 599
XapI RAATTY 4 cut(s) 318, 607, 698, 761
XceI RCATGY 1 cut(s) 268
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.