Rh2CG077800

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008176
Physical Location & Seq
Reverse (-)
6295717 .. 6301764
6048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG077800.1

Sequence Viewer

Length: 2832 bp
ATGCCATCGCCGCCTCAACAGGGTGCTCTTTATCCCTTCGATGAGGAATGCTTCATTTGCCTCCAGCCTTTTCGGTTCGGGGAGGTGGTGTCTTTTCCGTGCTCGGAAAACCACCACATGCATGGTAATTGTTACATCTTCGGTTGGGAGGTCCAGCTTTCAGAGAAAGGCCTTGACCAGTCGCATTGTCCTTTGTGCCGACAACAGATTGACTTCGGACGCTCTCACTGCTTCATCCATAAAGAGAGCGGGCTCATACCGGCGGTGACCATACCTTCAAATCCTCCGCCTCCACCGGCGGTTCCTCGCCACGTGTTCCCTCCCCCCGCGGTTCCTCCCCCAGCGGTTCCTCCCCCTCCCCCTCCCCGTCTTCCCCGCCGCGCTCTCGGTATCCCGCGGCCGCCGGCGTCCCCGACGCCCGCCAATGTCGAGAACCACATCCGGCGGTCGCGGGCATCGTGGCGCCAATACGTGGCCGAGCAGCACTACCGGAGGGTTTGGTTTGTGACGATGGCTTTTGCCCCTGACCCGGGCTATCAGGCCCTCAGCAAAGGCTTGGACATTATACAACAGCCATCTGAAGCCGAAGCCGGGCATCAGGAGTCTTTGGAGAAACGTATTGCTGAAGAATGGATGCCTTCTTCACGGAACATGACATATATGCTTGCGCAGTTCAAGCAGAAGGTTTCTATGCTTGATAACTCTGGGAAGCCAGTCCTTGCATCAACTGACAGTTCAAACCCCTGGTGTGCCCTGCTAGAAGAAGTGGTCAGTAAAGCTTACGGAACACTTGGTAAGCCTGAGATTTGTCCTGCTTCAACAGATGCTCACTATTTCCAGCTACAAGGCTTGCCAGCAATTGGATTCTCTCCTATGGTCAACACTCCTATTCTGCTCCATGACCATAACGAGGTCGTGGCTGTTGGAAGATTCCAAAATGGTGGTGCATTCAATGTTATTCCAGATTCTGCTGAACAAAGGGTTGAAGAGAGCTTGATTCGTGTTGGTTTGTTTTATTTTCTTTACAGGAACTGCCTAATTGATCTGGAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGACGTACCAGAACTCATGGATATCCACAAGCATTTCACAGCAAAATATGGAAAGGAGTTCGTCTCTGCAGCTGTTGAACTGCGCCCAGATAGTGGTGTAAGCCGTATGGTAATAGAAAAATTATCCACCAAATCACCTGATGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTGCTGAGGACCTGCTGAACGGACCAAATACTTTTGGCATGGCTTCTGCTACACCATCACCTACTCATGATGTCAGGGGACCTACTAATGTCCAAGTTCCTCCTAATAGTAGCATGGCCAATAAAGCTCATGTGGAAGCTCCCCCTGGTCCGCTTCCACCTGATAAACCTCCTAATGCTGAAGTTCCACGCAGAAATTATGAGTTTCAGGATGGACCCGTGAACTCTAATGAGCAGATTGCAAGATCATCACCTCGCTTGGAGGATTCTGCTTCTACAAATGTTTCTGCCAACAAAGCAACCACATCTACCACATTTCATCCAGAAACGATATCCTCAGCTAGTTTAGATCCTTGGAGTTTTGAGCAAAAGAGAGATGAGGTACCATTCAAGTCCGTTTCACCATTACGTTCTTCATCAGTGCAGCACTCCCTCCCCATCTTTTCTGAAGGTTTGACAGGTTCTACAGTCCCTTCGGAACCAAATGACTTCGTGCCTGTTACTTTTGATGCTTCAGATGGCCCAAGTTCAGACAGTGAGGAGGAGTTGGACACGTCTATGCTTGTCCTGAGCAAAGATTCTAAATTTTCTCATGAGAAGACTGTTCAATCCATAAATTCGGGAAAGAGCCAGAGTGCAAGCCACAGGTCATTAGCATCTTCATCCTCTGATGAGGTACATCCTGAGAGAACCCAGGGAATTGAATATAGTGTTTTCTCAGATAAAAGGTTTGATGATGATGAATTGCCCGGTAGTGAACCATCTCCAAGGCTGAAAAAATCTGGATTAGATACAAGTGCCAAGGATGATTTATCTCAAACAGTGAAAGACAGTGAGGAATGTAGTTGTGTAAGTGATACTGACAATGAGTTGTTGGCCTTTGGGGTCTTGAAAGGTGGCCTTCGGAACAAGGGTTATAGGCATCCACCATATAATCGGAATCCATCAGGCAATGCTTTTTTGGGCAAACAAATTACTCAAGATACTCCAACAGTCAGGACTTCCGATGTTTCTCATACGTCTATTGAAGAGCCATATAGCCGGAAAGGGAGCACTAAGCTAAATAAGGAACAAAGCACTAAAACCCCAGTCACACATGTCACTCCTGATGATGATTCTTCAGAGGATGAACTCTCACATGAAACTCTAAGCAGTAGTCGCCAAGACCTGTACAATCAGAAATCGGTCTCTAGAGAAGTACATGATGATTCTTCAGAGGATGAACTCTCACATGAAACACTTAGCAATAGTCGCCAAGACCTGTCCCATCATAAATCGGTATCCCAAGGAAAGACGAGCTCAACGTCTGCATTTTTCTTTAATTCAGAGGATAGTGAGGCTGAGGAAGATGTTCCTATAAAGATTTCCACCACCATTGCTGTCCAAGTTCAAAATTTTCTAGAAGGACGCAACCCTCTTCTTCAAATTCTGTTAGAATTAAGTTCTAGCATAAGGACAACAGTTGTTCCTGATGCACCAAGGACTTCAGATCCTCTTCAAGAAGTTCCAAAGACGCTGAAACTCTACCAGAGCCCTCAAGAAGTCAGGATTGGCAGAGACCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

943

Amino Acids

103.94

Weight (kDa)

5.26

Isoelectric Point (pI)

64.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 343 - 413 4e-20 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 669
Acc36I ACCTGC 1 cut(s) 1287
Acc65I GGTACC 1 cut(s) 1648
AccB1I GGYRCC 2 cut(s) 462, 1648
AccB7I CCANNNNNTGG 3 cut(s) 472, 860, 1184
AccBSI CCGCTC 1 cut(s) 249
AccII CGCG 4 cut(s) 329, 381, 397, 451
AclWI GGATC 4 cut(s) 1238, 1251, 1610, 2750
AcoI YGGCCR 3 cut(s) 398, 474, 1383
AcsI RAATTY 4 cut(s) 1850, 1882, 2659, 2691
AcuI CTGAAG 8 cut(s) 600, 645, 1467, 1734, 1764, 2370, 2463, 2736
AcvI CACGTG 1 cut(s) 313
AcyI GRCGYC 3 cut(s) 407, 416, 463
AfaI GTAC 5 cut(s) 1098, 1650, 1944, 2438, 2466
AfiI CCNNNNNNNGG 8 cut(s) 20, 472, 529, 530, 591, 860, 910, 1184
AflIII ACRYGT 3 cut(s) 312, 1818, 2362
AjiI CACGTC 1 cut(s) 1821
AjnI CCWGG 3 cut(s) 743, 1411, 1959
AjuI GAANNNNNNNTTGG 2 cut(s) 2799, 2831
Alw21I GWGCWC 4 cut(s) 28, 104, 2321, 2567
Alw26I GTCTC 3 cut(s) 1159, 2458, 2817
AlwI GGATC 4 cut(s) 1238, 1251, 1610, 2750
AlwNI CAGNNNCTG 2 cut(s) 968, 1032
Ama87I CYCGRG 2 cut(s) 529, 1247
AoxI GGCC 8 cut(s) 169, 398, 474, 540, 1383, 1786, 2142, 2164
ApeKI GCWGC 3 cut(s) 481, 1160, 1690
ApoI RAATTY 4 cut(s) 1850, 1882, 2659, 2691
Asp700I GAANNNNTTC 1 cut(s) 2616
Asp718I GGTACC 1 cut(s) 1648
AspLEI GCGC 4 cut(s) 383, 465, 670, 1176
AspS9I GGNCC 8 cut(s) 151, 541, 1276, 1289, 1346, 1415, 1481, 1787
AsuC2I CCSGG 4 cut(s) 530, 531, 592, 2016
AsuHPI GGTGA 5 cut(s) 277, 1218, 1317, 1509, 1659
AvaI CYCGRG 2 cut(s) 529, 1247
AvaII GGWCC 6 cut(s) 151, 1276, 1289, 1346, 1415, 1481
BaeGI GKGCMC 1 cut(s) 754
BalI TGGCCA 1 cut(s) 1385
BamHI GGATCC 1 cut(s) 1243
BanI GGYRCC 2 cut(s) 462, 1648
BanII GRGCYC 3 cut(s) 255, 2567, 2801
BbrPI CACGTG 1 cut(s) 313
BbsI GAAGAC 2 cut(s) 362, 1871
Bbv12I GWGCWC 4 cut(s) 28, 104, 2321, 2567
BbvCI CCTCAGC 4 cut(s) 545, 1272, 1603, 2607
BbvI GCAGC 3 cut(s) 493, 1172, 1702
BceAI ACGGC 1 cut(s) 1179
BciT130I CCWGG 3 cut(s) 745, 1413, 1961
BciVI GTATCC 2 cut(s) 401, 2557
BcnI CCSGG 4 cut(s) 530, 531, 592, 2016
BcoDI GTCTC 3 cut(s) 1159, 2458, 2817
BfaI CTAG 6 cut(s) 758, 1083, 1608, 2457, 2666, 2712
BfmI CTRYAG 3 cut(s) 1158, 1731, 2828
BfoI RGCGCY 1 cut(s) 466
BfuAI ACCTGC 1 cut(s) 1287
BfuI GTATCC 2 cut(s) 401, 2557
BisI GCNGC 7 cut(s) 11, 379, 398, 401, 482, 1161, 1691
BlsI GCNGC 7 cut(s) 12, 380, 399, 402, 483, 1162, 1692
Bme1390I CCNGG 7 cut(s) 530, 531, 592, 745, 1413, 1961, 2016
Bme18I GGWCC 6 cut(s) 151, 1276, 1289, 1346, 1415, 1481
BmeT110I CYCGRG 2 cut(s) 529, 1247
BmgBI CACGTC 1 cut(s) 1821
BmgT120I GGNCC 8 cut(s) 151, 541, 1276, 1289, 1346, 1415, 1481, 1787
BmiI GGNNCC 9 cut(s) 303, 333, 348, 464, 1245, 1347, 1483, 1650, 1746
BmrFI CCNGG 7 cut(s) 530, 531, 592, 745, 1413, 1961, 2016
BmrI ACTGGG 1 cut(s) 2348
BmuI ACTGGG 1 cut(s) 2348
BpiI GAAGAC 2 cut(s) 362, 1871
BplI GAGNNNNNCTC 2 cut(s) 1139, 1171
BpmI CTGGAG 2 cut(s) 47, 1067
Bpu10I CCTNAGC 5 cut(s) 545, 1272, 1603, 1835, 2607
BpuEI CTTGAG 2 cut(s) 2229, 2787
BpuMI CCSGG 4 cut(s) 530, 531, 592, 2016
BsaAI YACGTR 2 cut(s) 313, 472
BsaHI GRCGYC 3 cut(s) 407, 416, 463
BsaI GGTCTC 2 cut(s) 2458, 2817
BsaWI WCCGGW 1 cut(s) 489
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 8 cut(s) 20, 472, 529, 530, 591, 860, 910, 1184
Bse118I RCCGGY 3 cut(s) 259, 295, 403
Bse1I ACTGG 3 cut(s) 178, 713, 2354
Bse3DI GCAATG 2 cut(s) 2224, 2640
BseBI CCWGG 3 cut(s) 745, 1413, 1961
BseLI CCNNNNNNNGG 8 cut(s) 20, 472, 529, 530, 591, 860, 910, 1184
BseMI GCAATG 2 cut(s) 2224, 2640
BseMII CTCAG 8 cut(s) 559, 792, 1263, 1617, 1826, 1941, 1998, 2598
BseNI ACTGG 3 cut(s) 178, 713, 2354
BseRI GAGGAG 2 cut(s) 1820, 1823
BseSI GKGCMC 1 cut(s) 754
BseX3I CGGCCG 1 cut(s) 398
BseXI GCAGC 3 cut(s) 493, 1172, 1702
BseYI CCCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 1709
Bsh1236I CGCG 4 cut(s) 329, 381, 397, 451
Bsh1285I CGRYCG 2 cut(s) 401, 449
BshFI GGCC 8 cut(s) 171, 400, 476, 542, 1385, 1788, 2144, 2166
BshNI GGYRCC 2 cut(s) 462, 1648
BsiEI CGRYCG 2 cut(s) 401, 449
BsiHKAI GWGCWC 4 cut(s) 28, 104, 2321, 2567
BsiHKCI CYCGRG 2 cut(s) 529, 1247
BsiSI CCGG 9 cut(s) 260, 296, 404, 442, 490, 530, 591, 2016, 2308
BslFI GGGAC 4 cut(s) 394, 1359, 1721, 2515
BslI CCNNNNNNNGG 8 cut(s) 20, 472, 529, 530, 591, 860, 910, 1184
BsmAI GTCTC 3 cut(s) 1159, 2458, 2817
BsmBI CGTCTC 1 cut(s) 1159
BsmFI GGGAC 4 cut(s) 394, 1359, 1721, 2515
BsmI GAATGC 2 cut(s) 53, 947
BsnI GGCC 8 cut(s) 171, 400, 476, 542, 1385, 1788, 2144, 2166
Bso31I GGTCTC 2 cut(s) 2458, 2817
BsoBI CYCGRG 2 cut(s) 529, 1247
Bsp1286I GDGCHC 7 cut(s) 28, 104, 255, 754, 2321, 2567, 2801
Bsp1407I TGTACA 1 cut(s) 2436
Bsp143I GATC 5 cut(s) 1042, 1243, 1511, 1615, 2755
BspANI GGCC 8 cut(s) 171, 400, 476, 542, 1385, 1788, 2144, 2166
BspCNI CTCAG 8 cut(s) 558, 793, 1264, 1616, 1827, 1942, 1997, 2599
BspFNI CGCG 4 cut(s) 329, 381, 397, 451
BspHI TCATGA 2 cut(s) 1333, 1858
BspLI GGNNCC 9 cut(s) 303, 333, 348, 464, 1245, 1347, 1483, 1650, 1746
BspMAI CTGCAG 1 cut(s) 1162
BspMI ACCTGC 1 cut(s) 1287
BspPI GGATC 4 cut(s) 1238, 1251, 1610, 2750
BspQI GCTCTTC 1 cut(s) 2289
BspT107I GGYRCC 2 cut(s) 462, 1648
BspTNI GGTCTC 2 cut(s) 2458, 2817
BsrBI CCGCTC 1 cut(s) 249
BsrDI GCAATG 2 cut(s) 2224, 2640
BsrFI RCCGGY 3 cut(s) 259, 295, 403
BsrGI TGTACA 1 cut(s) 2436
BsrI ACTGG 3 cut(s) 178, 713, 2354
BssAI RCCGGY 3 cut(s) 259, 295, 403
BssMI GATC 5 cut(s) 1042, 1243, 1511, 1615, 2755
BssNI GRCGYC 3 cut(s) 407, 416, 463
BssT1I CCWWGG 5 cut(s) 1619, 2033, 2067, 2551, 2744
Bst2UI CCWGG 3 cut(s) 745, 1413, 1961
Bst4CI ACNGT 8 cut(s) 734, 1735, 1802, 1870, 2089, 2099, 2260, 2728
Bst6I CTCTTC 5 cut(s) 981, 1254, 2289, 2688, 2766
BstACI GRCGYC 3 cut(s) 407, 416, 463
BstAUI TGTACA 1 cut(s) 2436
BstBAI YACGTR 2 cut(s) 313, 472
BstC8I GCNNGC 9 cut(s) 251, 405, 420, 453, 666, 851, 855, 1269, 1906
BstDSI CCRYGG 2 cut(s) 327, 395
BstEII GGTNACC 1 cut(s) 265
BstFNI CGCG 4 cut(s) 329, 381, 397, 451
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 4 cut(s) 383, 465, 670, 1176
BstKTI GATC 5 cut(s) 1045, 1246, 1514, 1618, 2758
BstMAI GTCTC 3 cut(s) 1159, 2458, 2817
BstMBI GATC 5 cut(s) 1042, 1243, 1511, 1615, 2755
BstMCI CGRYCG 2 cut(s) 401, 449
BstMWI GCNNNNNNNGC 8 cut(s) 10, 57, 228, 676, 1391, 1562, 2424, 2517
BstNI CCWGG 3 cut(s) 745, 1413, 1961
BstNSI RCATGY 2 cut(s) 121, 2366
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 7 cut(s) 528, 529, 590, 743, 1411, 1959, 2014
BstSFI CTRYAG 3 cut(s) 1158, 1731, 2828
BstSLI GKGCMC 1 cut(s) 754
BstUI CGCG 4 cut(s) 329, 381, 397, 451
BstV1I GCAGC 3 cut(s) 493, 1172, 1702
BstV2I GAAGAC 2 cut(s) 362, 1871
BstX2I RGATCY 3 cut(s) 1243, 1615, 2755
BstXI CCANNNNNNTGG 2 cut(s) 122, 941
BstYI RGATCY 3 cut(s) 1243, 1615, 2755
BstZI CGGCCG 1 cut(s) 398
BsuI GTATCC 2 cut(s) 401, 2557
BsuRI GGCC 8 cut(s) 171, 400, 476, 542, 1385, 1788, 2144, 2166
BtgI CCRYGG 2 cut(s) 327, 395
BtrI CACGTC 1 cut(s) 1821
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 5 cut(s) 226, 1692, 1807, 2094, 2104
BveI ACCTGC 1 cut(s) 1287
Cac8I GCNNGC 9 cut(s) 251, 405, 420, 453, 666, 851, 855, 1269, 1906
CaiI CAGNNNCTG 2 cut(s) 968, 1032
CciI TCATGA 2 cut(s) 1333, 1858
CciNI GCGGCCGC 1 cut(s) 398
CfoI GCGC 4 cut(s) 383, 465, 670, 1176
Cfr10I RCCGGY 3 cut(s) 259, 295, 403
Cfr13I GGNCC 8 cut(s) 151, 541, 1276, 1289, 1346, 1415, 1481, 1787
Cfr42I CCGCGG 2 cut(s) 330, 398
Cfr9I CCCGGG 1 cut(s) 529
CseI GACGC 5 cut(s) 228, 396, 424, 2682, 2788
Csp6I GTAC 5 cut(s) 1097, 1649, 1943, 2437, 2465
CspCI CAANNNNNGTGG 2 cut(s) 2623, 2658
CviQI GTAC 5 cut(s) 1097, 1649, 1943, 2437, 2465
DinI GGCGCC 1 cut(s) 464
DpnI GATC 5 cut(s) 1044, 1245, 1513, 1617, 2757
DpnII GATC 5 cut(s) 1042, 1243, 1511, 1615, 2755
EaeI YGGCCR 3 cut(s) 398, 474, 1383
EagI CGGCCG 1 cut(s) 398
Eam1104I CTCTTC 5 cut(s) 981, 1254, 2289, 2688, 2766
EarI CTCTTC 5 cut(s) 981, 1254, 2289, 2688, 2766
EciI GGCGGA 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 2565
EclXI CGGCCG 1 cut(s) 398
Eco130I CCWWGG 5 cut(s) 1619, 2033, 2067, 2551, 2744
Eco147I AGGCCT 1 cut(s) 171
Eco24I GRGCYC 3 cut(s) 255, 2567, 2801
Eco31I GGTCTC 2 cut(s) 2458, 2817
Eco32I GATATC 2 cut(s) 1114, 1599
Eco47I GGWCC 6 cut(s) 151, 1276, 1289, 1346, 1415, 1481
Eco52I CGGCCG 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 2565
Eco57I CTGAAG 8 cut(s) 600, 645, 1467, 1734, 1764, 2370, 2463, 2736
Eco72I CACGTG 1 cut(s) 313
Eco88I CYCGRG 2 cut(s) 529, 1247
Eco91I GGTNACC 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 2565
EcoO109I RGGNCCY 3 cut(s) 541, 1276, 1346
EcoO65I GGTNACC 1 cut(s) 265
EcoRII CCWGG 3 cut(s) 743, 1411, 1959
EcoRV GATATC 2 cut(s) 1114, 1599
EcoT14I CCWWGG 5 cut(s) 1619, 2033, 2067, 2551, 2744
EcoT22I ATGCAT 1 cut(s) 123
EcoT38I GRGCYC 3 cut(s) 255, 2567, 2801
EgeI GGCGCC 1 cut(s) 464
EheI GGCGCC 1 cut(s) 464
ErhI CCWWGG 5 cut(s) 1619, 2033, 2067, 2551, 2744
Esp3I CGTCTC 1 cut(s) 1159
FalI AAGNNNNNCTT 2 cut(s) 2151, 2183
FaqI GGGAC 4 cut(s) 394, 1359, 1721, 2515
FauI CCCGC 6 cut(s) 242, 334, 383, 402, 427, 444
Fnu4HI GCNGC 7 cut(s) 11, 379, 398, 401, 482, 1161, 1691
FriOI GRGCYC 3 cut(s) 255, 2567, 2801
Fsp4HI GCNGC 7 cut(s) 11, 379, 398, 401, 482, 1161, 1691
FspBI CTAG 6 cut(s) 758, 1083, 1608, 2457, 2666, 2712
FspI TGCGCA 1 cut(s) 669
GlaI GCGC 4 cut(s) 382, 464, 669, 1175
GluI GCNGC 7 cut(s) 11, 379, 398, 401, 482, 1161, 1691
GsaI CCCAGC 1 cut(s) 344
GsuI CTGGAG 2 cut(s) 47, 1067
HaeII RGCGCY 1 cut(s) 466
HaeIII GGCC 8 cut(s) 171, 400, 476, 542, 1385, 1788, 2144, 2166
HapII CCGG 9 cut(s) 260, 296, 404, 442, 490, 530, 591, 2016, 2308
HgaI GACGC 5 cut(s) 228, 396, 424, 2682, 2788
HhaI GCGC 4 cut(s) 383, 465, 670, 1176
Hin1I GRCGYC 3 cut(s) 407, 416, 463
Hin6I GCGC 4 cut(s) 381, 463, 668, 1174
HinP1I GCGC 4 cut(s) 381, 463, 668, 1174
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 1 cut(s) 777
HpaII CCGG 9 cut(s) 260, 296, 404, 442, 490, 530, 591, 2016, 2308
HphI GGTGA 5 cut(s) 277, 1218, 1317, 1509, 1659
Hpy166II GTNNAC 3 cut(s) 880, 1489, 2024
Hpy8I GTNNAC 3 cut(s) 880, 1489, 2024
Hpy99I CGWCG 1 cut(s) 418
HpyAV CCTTC 8 cut(s) 46, 285, 648, 676, 1709, 1749, 2177, 2663
HpyCH4III ACNGT 8 cut(s) 734, 1735, 1802, 1870, 2089, 2099, 2260, 2728
HpyCH4IV ACGT 8 cut(s) 312, 471, 616, 1095, 1675, 1820, 2285, 2570
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 57, 228, 676, 1391, 1562, 2424, 2517
HpySE526I ACGT 8 cut(s) 312, 471, 616, 1095, 1675, 1820, 2285, 2570
Hsp92I GRCGYC 3 cut(s) 407, 416, 463
HspAI GCGC 4 cut(s) 381, 463, 668, 1174
KasI GGCGCC 1 cut(s) 462
KpnI GGTACC 1 cut(s) 1652
KroI GCCGGC 1 cut(s) 403
KroNI GCCGGC 1 cut(s) 405
KspI CCGCGG 2 cut(s) 330, 398
Kzo9I GATC 5 cut(s) 1042, 1243, 1511, 1615, 2755
LguI GCTCTTC 1 cut(s) 2289
LmnI GCTCC 3 cut(s) 900, 1411, 2316
Lsp1109I GCAGC 3 cut(s) 493, 1172, 1702
MaeI CTAG 6 cut(s) 758, 1083, 1608, 2457, 2666, 2712
MaeII ACGT 8 cut(s) 312, 471, 616, 1095, 1675, 1820, 2285, 2570
MaeIII GTNAC 6 cut(s) 131, 265, 505, 1765, 2356, 2365
MalI GATC 5 cut(s) 1044, 1245, 1513, 1617, 2757
MbiI CCGCTC 1 cut(s) 249
MboI GATC 5 cut(s) 1042, 1243, 1511, 1615, 2755
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 3 cut(s) 1243, 1615, 2755
MhlI GDGCHC 7 cut(s) 28, 104, 255, 754, 2321, 2567, 2801
MlsI TGGCCA 1 cut(s) 1385
MluNI TGGCCA 1 cut(s) 1385
Mly113I GGCGCC 1 cut(s) 463
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 3 cut(s) 904, 1794, 2279
Mox20I TGGCCA 1 cut(s) 1385
Mph1103I ATGCAT 1 cut(s) 123
MreI CGCCGGCG 1 cut(s) 403
MroNI GCCGGC 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 2616
MscI TGGCCA 1 cut(s) 1385
MseI TTAA 2 cut(s) 2586, 2705
MslI CAYNNNNRTG 2 cut(s) 120, 1823
Msp20I TGGCCA 1 cut(s) 1385
MspA1I CMGCKG 4 cut(s) 329, 344, 397, 1163
MspI CCGG 9 cut(s) 260, 296, 404, 442, 490, 530, 591, 2016, 2308
MspR9I CCNGG 7 cut(s) 530, 531, 592, 745, 1413, 1961, 2016
MunI CAATTG 1 cut(s) 858
Mva1269I GAATGC 2 cut(s) 53, 947
MvaI CCWGG 3 cut(s) 745, 1413, 1961
MvnI CGCG 4 cut(s) 329, 381, 397, 451
MwoI GCNNNNNNNGC 8 cut(s) 10, 57, 228, 676, 1391, 1562, 2424, 2517
NaeI GCCGGC 1 cut(s) 405
NarI GGCGCC 1 cut(s) 463
NciI CCSGG 4 cut(s) 530, 531, 592, 2016
NdeII GATC 5 cut(s) 1042, 1243, 1511, 1615, 2755
NgoMIV GCCGGC 1 cut(s) 403
NlaIV GGNNCC 9 cut(s) 303, 333, 348, 464, 1245, 1347, 1483, 1650, 1746
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 4 cut(s) 265, 505, 2356, 2365
NotI GCGGCCGC 1 cut(s) 398
NsbI TGCGCA 1 cut(s) 669
NsiI ATGCAT 1 cut(s) 123
NspI RCATGY 2 cut(s) 121, 2366
PagI TCATGA 2 cut(s) 1333, 1858
PasI CCCWGGG 1 cut(s) 1960
PceI AGGCCT 1 cut(s) 171
PciI ACATGT 1 cut(s) 2362
PciSI GCTCTTC 1 cut(s) 2289
PcsI WCGNNNNNNNCGW 2 cut(s) 455, 2567
PctI GAATGC 2 cut(s) 53, 947
PdiI GCCGGC 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 2616
PfeI GAWTC 9 cut(s) 864, 930, 965, 997, 1532, 1844, 2206, 2381, 2474
PflMI CCANNNNNTGG 3 cut(s) 472, 860, 1184
PkrI GCNGC 7 cut(s) 12, 380, 399, 402, 483, 1162, 1692
PleI GAGTC 1 cut(s) 610
PluTI GGCGCC 1 cut(s) 466
PmaCI CACGTG 1 cut(s) 313
PmlI CACGTG 1 cut(s) 313
PpsI GAGTC 1 cut(s) 610
Ppu21I YACGTR 2 cut(s) 313, 472
PpuMI RGGWCCY 2 cut(s) 1276, 1346
PscI ACATGT 1 cut(s) 2362
Psp124BI GAGCTC 1 cut(s) 2567
Psp5II RGGWCCY 2 cut(s) 1276, 1346
Psp6I CCWGG 3 cut(s) 743, 1411, 1959
PspCI CACGTG 1 cut(s) 313
PspEI GGTNACC 1 cut(s) 265
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 3 cut(s) 743, 1411, 1959
PspN4I GGNNCC 9 cut(s) 303, 333, 348, 464, 1245, 1347, 1483, 1650, 1746
PspPI GGNCC 8 cut(s) 151, 541, 1276, 1289, 1346, 1415, 1481, 1787
PspPPI RGGWCCY 2 cut(s) 1276, 1346
PstI CTGCAG 1 cut(s) 1162
PstNI CAGNNNCTG 2 cut(s) 968, 1032
PsuI RGATCY 3 cut(s) 1243, 1615, 2755
PvuII CAGCTG 1 cut(s) 1163
RsaI GTAC 5 cut(s) 1098, 1650, 1944, 2438, 2466
RsaNI GTAC 5 cut(s) 1097, 1649, 1943, 2437, 2465
RseI CAYNNNNRTG 2 cut(s) 120, 1823
SacI GAGCTC 1 cut(s) 2567
SacII CCGCGG 2 cut(s) 330, 398
SapI GCTCTTC 1 cut(s) 2289
SaqAI TTAA 2 cut(s) 2586, 2705
SatI GCNGC 7 cut(s) 11, 379, 398, 401, 482, 1161, 1691
Sau3AI GATC 5 cut(s) 1042, 1243, 1511, 1615, 2755
Sau96I GGNCC 8 cut(s) 151, 541, 1276, 1289, 1346, 1415, 1481, 1787
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 7 cut(s) 530, 531, 592, 745, 1413, 1961, 2016
SduI GDGCHC 7 cut(s) 28, 104, 255, 754, 2321, 2567, 2801
SfcI CTRYAG 3 cut(s) 1158, 1731, 2828
SfoI GGCGCC 1 cut(s) 464
Sfr303I CCGCGG 2 cut(s) 330, 398
SgrAI CRCCGGYG 2 cut(s) 295, 403
SgrBI CCGCGG 2 cut(s) 330, 398
SinI GGWCC 6 cut(s) 151, 1276, 1289, 1346, 1415, 1481
SmaI CCCGGG 1 cut(s) 531
SmiMI CAYNNNNRTG 2 cut(s) 120, 1823
SmlI CTYRAG 2 cut(s) 2244, 2802
SmoI CTYRAG 2 cut(s) 2244, 2802
SseBI AGGCCT 1 cut(s) 171
SspDI GGCGCC 1 cut(s) 462
SspMI CTAG 6 cut(s) 758, 1083, 1608, 2457, 2666, 2712
SstI GAGCTC 1 cut(s) 2567
StuI AGGCCT 1 cut(s) 171
StyD4I CCNGG 7 cut(s) 528, 529, 590, 743, 1411, 1959, 2014
StyI CCWWGG 5 cut(s) 1619, 2033, 2067, 2551, 2744
TaaI ACNGT 8 cut(s) 734, 1735, 1802, 1870, 2089, 2099, 2260, 2728
TaiI ACGT 8 cut(s) 315, 474, 619, 1098, 1678, 1823, 2288, 2573
TaqI TCGA 2 cut(s) 39, 429
TaqII GACCGA 1 cut(s) 2440
TatI WGTACW 2 cut(s) 2436, 2464
TauI GCSGC 4 cut(s) 13, 381, 400, 403
TfiI GAWTC 9 cut(s) 864, 930, 965, 997, 1532, 1844, 2206, 2381, 2474
Tru1I TTAA 2 cut(s) 2586, 2705
Tru9I TTAA 2 cut(s) 2586, 2705
TscAI CASTG 5 cut(s) 233, 1692, 1807, 2094, 2104
TseFI GTSAC 4 cut(s) 265, 505, 2356, 2365
TseI GCWGC 3 cut(s) 481, 1160, 1690
Tsp45I GTSAC 4 cut(s) 265, 505, 2356, 2365
TspGWI ACGGA 5 cut(s) 87, 661, 798, 1302, 1651
TspMI CCCGGG 1 cut(s) 529
TspRI CASTG 5 cut(s) 233, 1692, 1807, 2094, 2104
Van91I CCANNNNNTGG 3 cut(s) 472, 860, 1184
VpaK11BI GGWCC 6 cut(s) 151, 1276, 1289, 1346, 1415, 1481
XapI RAATTY 4 cut(s) 1850, 1882, 2659, 2691
XbaI TCTAGA 2 cut(s) 2456, 2665
XceI RCATGY 2 cut(s) 121, 2366
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XmaI CCCGGG 1 cut(s) 529
XmnI GAANNNNTTC 1 cut(s) 2616
XspI CTAG 6 cut(s) 758, 1083, 1608, 2457, 2666, 2712
Zsp2I ATGCAT 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.