Rroxscaffold_2G00148880

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008176
Physical Location & Seq
Forward (+)
86509810 .. 86520650
10841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148880.1

Sequence Viewer

Length: 1287 bp
ATGCCGGCAAATGTGGAGAACCGCCTCCGGCGGTCGCGGGCATCCTGGGGCCCATACCTGGCCACGCAGCAACACCACAGGCCATCTGAAGCCGAAGCCGGGCGTCTGGAGTCTTTGGAGAAACGTATTGCTGAAGAATGGACGCCTTCTTCACGGAACATGACATATATGCTTGGGCAGTTCAAGCAGAAGGTCTCTATGCTTGATAACTCTGGGAAGCCAGTCCTTGCAGCAACTGACAGTTCAAACCCCTGGTGCGCCCTGCTAGAAGAAGTGGTCAGTAAAGCTTACGGAACACTTGGTAAGCCTGAGATTTGTCCTGCTTCAACAGATGCTCACTATTTCCAGCTACAAGGCTTGCCAGCAATTGGATTCTCTCCTATGGCCAACACTCCTATTCTGCTCCATGACCAAAACGAGGTTGTGGCTGTTGGAAGATTCCAAAATGGTGGTGCATTCAATGTTATTCCAGATTCTGCTGAACAAAGGGTTGAAGAGGTTGAACATGTGGTAAGGGAAGAGAAGTCGAGGGAAGCATATGAGCTTATCGAGATATATTGCGAACTTATTGCAGCACGATTGCCAATGTTTGAGTCGCAGAAGAACTGCCTAATTGATCTGGAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGTACGTACCAGAACTCATGGATATCCACAAGCATTTCACAGCAAAATATGGAAAGGAGTTCGTCTCTGCAGCTGTTGAACTGCGCCCAGATAGTGGTGTAAGCCGCATGGTAATAGAAAAATTATCCACCAAATCACCTGATGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTGCTGAGGACCTGCTGAACGGACCAAATACTTTTGGCATGGCTTCTGCTACACCATCACCTACTCATGATGTCAGGGGACCTACTAATGTCCAAGTTCCTCCTAATAGTAGCATGGCCAATAAAGCTCATGTGGAAGCTCCCCCTGGTCCGCTTCCACCTGATAAACCTCCTAATGCTGAAGTTCCACGCAGAAATAATGAGTTTCAGGATGGACCCGTGAACTCTAATGACCAGAATGCAAGATCATCACCTCGCTTGAAGGATTCTGCTTCTACAAATGTTTCTGCCAACAAAGCAACCACATCTACCACATTTCATCCAGAAACGATATCCTCAGGAAGTGATCGAATGGAAATGCATGGGGATGATAATTCTTTGGGCAGGCAGAACTGGAATATGCAATTTAAGGGTACTGCCTCTGCAGCACAGGCAGCTGCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

428

Amino Acids

47.0

Weight (kDa)

5.37

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 160 - 271 2.3e-34 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 861
AccB7I CCANNNNNTGG 2 cut(s) 368, 758
AccII CGCG 1 cut(s) 37
AciI CCGC 5 cut(s) 22, 31, 37, 769, 992
AclWI GGATC 2 cut(s) 812, 825
AcoI YGGCCR 3 cut(s) 60, 384, 957
AcuI CTGAAG 3 cut(s) 108, 153, 1041
AcyI GRCGYC 2 cut(s) 103, 143
AfaI GTAC 3 cut(s) 668, 672, 1255
AfiI CCNNNNNNNGG 5 cut(s) 58, 99, 368, 418, 758
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 9 cut(s) 184, 246, 327, 460, 494, 503, 743, 833, 1102
AjnI CCWGG 4 cut(s) 44, 57, 251, 985
AluBI AGCT 7 cut(s) 287, 349, 544, 737, 968, 980, 1277
AluI AGCT 7 cut(s) 287, 349, 544, 737, 968, 980, 1277
Alw26I GTCTC 2 cut(s) 199, 733
AlwI GGATC 2 cut(s) 812, 825
AlwNI CAGNNNCTG 3 cut(s) 236, 476, 1280
Ama87I CYCGRG 1 cut(s) 821
AoxI GGCC 5 cut(s) 49, 60, 80, 384, 957
ApaI GGGCCC 1 cut(s) 53
ApeKI GCWGC 7 cut(s) 67, 230, 572, 734, 1265, 1274, 1277
AspLEI GCGC 2 cut(s) 260, 750
AspS9I GGNCC 7 cut(s) 49, 50, 850, 863, 920, 989, 1055
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 3 cut(s) 792, 891, 1083
AvaI CYCGRG 1 cut(s) 821
AvaII GGWCC 5 cut(s) 850, 863, 920, 989, 1055
AxyI CCTNAGG 1 cut(s) 1177
BaeGI GKGCMC 1 cut(s) 53
BalI TGGCCA 3 cut(s) 62, 386, 959
BamHI GGATCC 1 cut(s) 817
BanII GRGCYC 1 cut(s) 53
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 6 cut(s) 79, 242, 584, 746, 1264, 1277
BccI CCATC 3 cut(s) 91, 904, 1046
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 4 cut(s) 46, 59, 253, 987
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 2 cut(s) 199, 733
BfaI CTAG 2 cut(s) 266, 657
BfmI CTRYAG 2 cut(s) 732, 1263
BfuAI ACCTGC 1 cut(s) 861
BisI GCNGC 8 cut(s) 68, 231, 573, 735, 769, 1266, 1275, 1278
BlsI GCNGC 8 cut(s) 69, 232, 574, 736, 770, 1267, 1276, 1279
Bme1390I CCNGG 5 cut(s) 46, 59, 100, 253, 987
Bme18I GGWCC 5 cut(s) 850, 863, 920, 989, 1055
BmeT110I CYCGRG 1 cut(s) 821
BmgT120I GGNCC 7 cut(s) 49, 50, 850, 863, 920, 989, 1055
BmiI GGNNCC 5 cut(s) 50, 51, 819, 921, 1057
BmrFI CCNGG 5 cut(s) 46, 59, 100, 253, 987
BmsI GCATC 3 cut(s) 50, 322, 659
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BpmI CTGGAG 2 cut(s) 128, 641
Bpu10I CCTNAGC 1 cut(s) 846
BpuMI CCSGG 1 cut(s) 100
BsaAI YACGTR 1 cut(s) 670
BsaHI GRCGYC 2 cut(s) 103, 143
BsaI GGTCTC 1 cut(s) 199
BsaJI CCNNGG 4 cut(s) 45, 251, 822, 985
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 99, 368, 418, 758
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 2 cut(s) 221, 1238
Bse21I CCTNAGG 1 cut(s) 1177
BseBI CCWGG 4 cut(s) 46, 59, 253, 987
BseDI CCNNGG 4 cut(s) 45, 251, 822, 985
BseGI GGATG 4 cut(s) 41, 1057, 1159, 1213
BseLI CCNNNNNNNGG 5 cut(s) 58, 99, 368, 418, 758
BseMII CTCAG 3 cut(s) 300, 837, 1191
BseNI ACTGG 2 cut(s) 221, 1238
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 6 cut(s) 79, 242, 584, 746, 1264, 1277
Bsh1236I CGCG 1 cut(s) 37
Bsh1285I CGRYCG 1 cut(s) 35
BshFI GGCC 5 cut(s) 51, 62, 82, 386, 959
BsiEI CGRYCG 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 821
BsiSI CCGG 3 cut(s) 5, 28, 99
BsiWI CGTACG 1 cut(s) 666
BslFI GGGAC 1 cut(s) 933
BslI CCNNNNNNNGG 5 cut(s) 58, 99, 368, 418, 758
BsmAI GTCTC 2 cut(s) 199, 733
BsmBI CGTCTC 1 cut(s) 733
BsmFI GGGAC 1 cut(s) 933
BsmI GAATGC 2 cut(s) 455, 1084
BsnI GGCC 5 cut(s) 51, 62, 82, 386, 959
Bso31I GGTCTC 1 cut(s) 199
BsoBI CYCGRG 1 cut(s) 821
Bsp120I GGGCCC 1 cut(s) 49
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 4 cut(s) 616, 817, 1085, 1186
BspACI CCGC 5 cut(s) 22, 31, 37, 769, 992
BspANI GGCC 5 cut(s) 51, 62, 82, 386, 959
BspCNI CTCAG 3 cut(s) 301, 838, 1190
BspFNI CGCG 1 cut(s) 37
BspHI TCATGA 1 cut(s) 907
BspLI GGNNCC 5 cut(s) 50, 51, 819, 921, 1057
BspMAI CTGCAG 2 cut(s) 736, 1267
BspMI ACCTGC 1 cut(s) 861
BspPI GGATC 2 cut(s) 812, 825
BspTNI GGTCTC 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 2 cut(s) 221, 1238
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 4 cut(s) 45, 251, 822, 985
BssMI GATC 4 cut(s) 616, 817, 1085, 1186
BssNI GRCGYC 2 cut(s) 103, 143
Bst2UI CCWGG 4 cut(s) 46, 59, 253, 987
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 3 cut(s) 489, 513, 828
BstACI GRCGYC 2 cut(s) 103, 143
BstBAI YACGTR 1 cut(s) 670
BstC8I GCNNGC 6 cut(s) 6, 39, 359, 363, 843, 1226
BstDEI CTNAG 3 cut(s) 309, 846, 1177
BstF5I GGATG 4 cut(s) 41, 1057, 1159, 1213
BstFNI CGCG 1 cut(s) 37
BstHHI GCGC 2 cut(s) 260, 750
BstKTI GATC 4 cut(s) 619, 820, 1088, 1189
BstMAI GTCTC 2 cut(s) 199, 733
BstMBI GATC 4 cut(s) 616, 817, 1085, 1186
BstMCI CGRYCG 1 cut(s) 35
BstMWI GCNNNNNNNGC 6 cut(s) 184, 965, 1136, 1265, 1271, 1274
BstNI CCWGG 4 cut(s) 46, 59, 253, 987
BstNSI RCATGY 1 cut(s) 509
BstSCI CCNGG 5 cut(s) 44, 57, 98, 251, 985
BstSFI CTRYAG 2 cut(s) 732, 1263
BstSLI GKGCMC 1 cut(s) 53
BstSNI TACGTA 1 cut(s) 670
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 6 cut(s) 79, 242, 584, 746, 1264, 1277
BstX2I RGATCY 1 cut(s) 817
BstXI CCANNNNNNTGG 1 cut(s) 449
BstYI RGATCY 1 cut(s) 817
Bsu36I CCTNAGG 1 cut(s) 1177
BsuRI GGCC 5 cut(s) 51, 62, 82, 386, 959
BtsCI GGATG 4 cut(s) 41, 1057, 1159, 1213
BveI ACCTGC 1 cut(s) 861
Cac8I GCNNGC 6 cut(s) 6, 39, 359, 363, 843, 1226
CaiI CAGNNNCTG 3 cut(s) 236, 476, 1280
CciI TCATGA 1 cut(s) 907
CfoI GCGC 2 cut(s) 260, 750
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 7 cut(s) 49, 50, 850, 863, 920, 989, 1055
CseI GACGC 2 cut(s) 92, 151
Csp6I GTAC 3 cut(s) 667, 671, 1254
CviQI GTAC 3 cut(s) 667, 671, 1254
DdeI CTNAG 3 cut(s) 309, 846, 1177
DpnI GATC 4 cut(s) 618, 819, 1087, 1188
DpnII GATC 4 cut(s) 616, 817, 1085, 1186
EaeI YGGCCR 3 cut(s) 60, 384, 957
Eam1104I CTCTTC 3 cut(s) 489, 513, 828
EarI CTCTTC 3 cut(s) 489, 513, 828
Eco105I TACGTA 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 199
Eco32I GATATC 2 cut(s) 688, 1173
Eco47I GGWCC 5 cut(s) 850, 863, 920, 989, 1055
Eco57I CTGAAG 3 cut(s) 108, 153, 1041
Eco81I CCTNAGG 1 cut(s) 1177
Eco88I CYCGRG 1 cut(s) 821
EcoO109I RGGNCCY 3 cut(s) 49, 850, 920
EcoRII CCWGG 4 cut(s) 44, 57, 251, 985
EcoRV GATATC 2 cut(s) 688, 1173
EcoT22I ATGCAT 1 cut(s) 1203
EcoT38I GRGCYC 1 cut(s) 53
Esp3I CGTCTC 1 cut(s) 733
FaqI GGGAC 1 cut(s) 933
FauI CCCGC 1 cut(s) 30
FauNDI CATATG 1 cut(s) 538
Fnu4HI GCNGC 8 cut(s) 68, 231, 573, 735, 769, 1266, 1275, 1278
FokI GGATG 4 cut(s) 28, 1064, 1146, 1220
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 8 cut(s) 68, 231, 573, 735, 769, 1266, 1275, 1278
FspBI CTAG 2 cut(s) 266, 657
GlaI GCGC 2 cut(s) 259, 749
GluI GCNGC 8 cut(s) 68, 231, 573, 735, 769, 1266, 1275, 1278
GsuI CTGGAG 2 cut(s) 128, 641
HaeIII GGCC 5 cut(s) 51, 62, 82, 386, 959
HapII CCGG 3 cut(s) 5, 28, 99
HgaI GACGC 2 cut(s) 92, 151
HhaI GCGC 2 cut(s) 260, 750
Hin1I GRCGYC 2 cut(s) 103, 143
Hin6I GCGC 2 cut(s) 258, 748
HinP1I GCGC 2 cut(s) 258, 748
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 6 cut(s) 110, 372, 438, 473, 593, 1106
HpaII CCGG 3 cut(s) 5, 28, 99
HphI GGTGA 3 cut(s) 792, 891, 1083
Hpy166II GTNNAC 1 cut(s) 1063
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 9 cut(s) 107, 470, 550, 620, 821, 908, 1049, 1163, 1179
Hpy8I GTNNAC 1 cut(s) 1063
HpyAV CCTTC 3 cut(s) 156, 184, 1096
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 2 cut(s) 124, 669
HpyCH4V TGCA 9 cut(s) 230, 455, 572, 650, 734, 1082, 1201, 1243, 1265
HpyF10VI GCNNNNNNNGC 6 cut(s) 184, 965, 1136, 1265, 1271, 1274
HpyF3I CTNAG 3 cut(s) 309, 846, 1177
HpySE526I ACGT 2 cut(s) 124, 669
Hsp92I GRCGYC 2 cut(s) 103, 143
HspAI GCGC 2 cut(s) 258, 748
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 4 cut(s) 616, 817, 1085, 1186
LmnI GCTCC 2 cut(s) 408, 985
Lsp1109I GCAGC 6 cut(s) 79, 242, 584, 746, 1264, 1277
LweI GCATC 3 cut(s) 50, 322, 659
MaeI CTAG 2 cut(s) 266, 657
MaeII ACGT 2 cut(s) 124, 669
MalI GATC 4 cut(s) 618, 819, 1087, 1188
MboI GATC 4 cut(s) 616, 817, 1085, 1186
MboII GAAGA 8 cut(s) 141, 146, 281, 447, 506, 530, 613, 845
MfeI CAATTG 1 cut(s) 366
MflI RGATCY 1 cut(s) 817
MhlI GDGCHC 1 cut(s) 53
MlsI TGGCCA 3 cut(s) 62, 386, 959
MluCI AATT 6 cut(s) 366, 612, 642, 785, 1213, 1244
MluNI TGGCCA 3 cut(s) 62, 386, 959
MlyI GAGTC 2 cut(s) 119, 602
MmeI TCCRAC 1 cut(s) 412
Mox20I TGGCCA 3 cut(s) 62, 386, 959
Mph1103I ATGCAT 1 cut(s) 1203
MroNI GCCGGC 1 cut(s) 4
MscI TGGCCA 3 cut(s) 62, 386, 959
MseI TTAA 1 cut(s) 1248
MslI CAYNNNNRTG 1 cut(s) 1206
Msp20I TGGCCA 3 cut(s) 62, 386, 959
MspA1I CMGCKG 2 cut(s) 737, 1277
MspI CCGG 3 cut(s) 5, 28, 99
MspR9I CCNGG 5 cut(s) 46, 59, 100, 253, 987
MunI CAATTG 1 cut(s) 366
Mva1269I GAATGC 2 cut(s) 455, 1084
MvaI CCWGG 4 cut(s) 46, 59, 253, 987
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 6 cut(s) 184, 965, 1136, 1265, 1271, 1274
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 1 cut(s) 100
NdeI CATATG 1 cut(s) 538
NdeII GATC 4 cut(s) 616, 817, 1085, 1186
NgoMIV GCCGGC 1 cut(s) 4
NlaIV GGNNCC 5 cut(s) 50, 51, 819, 921, 1057
NsiI ATGCAT 1 cut(s) 1203
NspI RCATGY 1 cut(s) 509
PagI TCATGA 1 cut(s) 907
PciI ACATGT 1 cut(s) 505
PctI GAATGC 2 cut(s) 455, 1084
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 4 cut(s) 372, 438, 473, 1106
Pfl23II CGTACG 1 cut(s) 666
PflMI CCANNNNNTGG 2 cut(s) 368, 758
PkrI GCNGC 8 cut(s) 69, 232, 574, 736, 770, 1267, 1276, 1279
PleI GAGTC 2 cut(s) 118, 601
PpsI GAGTC 2 cut(s) 118, 601
Ppu21I YACGTR 1 cut(s) 670
PpuMI RGGWCCY 2 cut(s) 850, 920
PscI ACATGT 1 cut(s) 505
Psp5II RGGWCCY 2 cut(s) 850, 920
Psp6I CCWGG 4 cut(s) 44, 57, 251, 985
PspGI CCWGG 4 cut(s) 44, 57, 251, 985
PspLI CGTACG 1 cut(s) 666
PspN4I GGNNCC 5 cut(s) 50, 51, 819, 921, 1057
PspOMI GGGCCC 1 cut(s) 49
PspPI GGNCC 7 cut(s) 49, 50, 850, 863, 920, 989, 1055
PspPPI RGGWCCY 2 cut(s) 850, 920
PstI CTGCAG 2 cut(s) 736, 1267
PstNI CAGNNNCTG 3 cut(s) 236, 476, 1280
PsuI RGATCY 1 cut(s) 817
PvuII CAGCTG 2 cut(s) 737, 1277
RsaI GTAC 3 cut(s) 668, 672, 1255
RsaNI GTAC 3 cut(s) 667, 671, 1254
RseI CAYNNNNRTG 1 cut(s) 1206
SaqAI TTAA 1 cut(s) 1248
SatI GCNGC 8 cut(s) 68, 231, 573, 735, 769, 1266, 1275, 1278
Sau3AI GATC 4 cut(s) 616, 817, 1085, 1186
Sau96I GGNCC 7 cut(s) 49, 50, 850, 863, 920, 989, 1055
SchI GAGTC 2 cut(s) 119, 602
ScrFI CCNGG 5 cut(s) 46, 59, 100, 253, 987
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 3 cut(s) 50, 322, 659
SfcI CTRYAG 2 cut(s) 732, 1263
SinI GGWCC 5 cut(s) 850, 863, 920, 989, 1055
SmiMI CAYNNNNRTG 1 cut(s) 1206
SnaBI TACGTA 1 cut(s) 670
Sse9I AATT 6 cut(s) 366, 612, 642, 785, 1213, 1244
SsiI CCGC 5 cut(s) 22, 31, 37, 769, 992
SspMI CTAG 2 cut(s) 266, 657
StyD4I CCNGG 5 cut(s) 44, 57, 98, 251, 985
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 2 cut(s) 127, 672
TaqI TCGA 3 cut(s) 527, 549, 1189
TasI AATT 6 cut(s) 366, 612, 642, 785, 1213, 1244
TauI GCSGC 1 cut(s) 771
TfiI GAWTC 4 cut(s) 372, 438, 473, 1106
Tru1I TTAA 1 cut(s) 1248
Tru9I TTAA 1 cut(s) 1248
TseI GCWGC 7 cut(s) 67, 230, 572, 734, 1265, 1274, 1277
TspDTI ATGAA 1 cut(s) 1148
TspGWI ACGGA 3 cut(s) 169, 306, 876
Van91I CCANNNNNTGG 2 cut(s) 368, 758
VpaK11BI GGWCC 5 cut(s) 850, 863, 920, 989, 1055
XceI RCATGY 1 cut(s) 509
XspI CTAG 2 cut(s) 266, 657
Zsp2I ATGCAT 1 cut(s) 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.