Rh2AG074800

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008176
Physical Location & Seq
Reverse (-)
6259372 .. 6262570
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG074800.1

Sequence Viewer

Length: 729 bp
ATGGCCGCCAACCGCCTTGCCGTATCTTCTCTGCCGCAGCGAAGAAGGACCATGTCCGCTCTCGAAGCCCGAATCTCCCTCGTTGCCGCTCTCACCACCCAAGCCTCCTCCGTCTCCTATTTTACTCTATTTGGCCACCAAGACAGCCAAATTACAATATGGGTTAGCAGTATATCGTTTGATACATATGCTGATATTGCATCTAAGGGCACAACTGATATTGAAAATGAGTATGGACCCATGGAATACGAAATTGGTGCACCAGGTAAAGCCTACACTGACTGGACAGCTCAAATGGTTGTGGGATTAAACACTAGTTTCCCCTGGATCCTGTGCAAACAAGATGATGCCCCTGTCCTGTTAAACTGCCCAATTGATCTGAAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGACGTACCAGAACTCATGGATATTCGCAAACACTTCACAGCAAAATATGGAAAGGAGTTCGTCTCTATAGCTGTTGAACTACGGCCGGATAGTGGTGTAAGCCGCATGTTAATAGAGAAATTATCTACCAAATCACCTGATGGCCCTACCAAGATGAAAATTTTGGCTGCATTTGCAGAGGAACATAATATCAAATGGGATCCCGAGACATTTGAAGAGAAGCCTCCTAAGGACCTGCTGGTTAAAATGGTTCAGCTCTTATTCATTTATTCATTGCTTGATATAGATGACATAAAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

242

Amino Acids

26.96

Weight (kDa)

5.08

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 122 - 207 2e-25 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 669
AccBSI CCGCTC 2 cut(s) 59, 89
AciI CCGC 7 cut(s) 6, 13, 35, 57, 87, 425, 529
AclWI GGATC 4 cut(s) 322, 335, 620, 633
AcoI YGGCCR 3 cut(s) 3, 133, 509
AcsI RAATTY 1 cut(s) 585
AcuI CTGAAG 1 cut(s) 401
AfaI GTAC 1 cut(s) 432
AfiI CCNNNNNNNGG 1 cut(s) 518
AgsI TTSAA 3 cut(s) 224, 503, 641
AhlI ACTAGT 1 cut(s) 314
AjnI CCWGG 2 cut(s) 262, 323
AjuI GAANNNNNNNTTGG 2 cut(s) 237, 269
AluBI AGCT 3 cut(s) 290, 497, 682
AluI AGCT 3 cut(s) 290, 497, 682
Alw21I GWGCWC 1 cut(s) 262
Alw26I GTCTC 3 cut(s) 118, 493, 626
Alw44I GTGCAC 1 cut(s) 258
AlwI GGATC 4 cut(s) 322, 335, 620, 633
Ama87I CYCGRG 1 cut(s) 629
AoxI GGCC 4 cut(s) 3, 133, 509, 568
ApaLI GTGCAC 1 cut(s) 258
ApeKI GCWGC 2 cut(s) 37, 593
ApoI RAATTY 1 cut(s) 585
AspS9I GGNCC 4 cut(s) 48, 236, 569, 658
AsuHPI GGTGA 2 cut(s) 85, 552
AvaI CYCGRG 1 cut(s) 629
AvaII GGWCC 3 cut(s) 48, 236, 658
AxyI CCTNAGG 1 cut(s) 654
BaeGI GKGCMC 2 cut(s) 212, 262
BalI TGGCCA 1 cut(s) 135
BamHI GGATCC 2 cut(s) 327, 625
Bbv12I GWGCWC 1 cut(s) 262
BbvI GCAGC 2 cut(s) 49, 580
BccI CCATC 1 cut(s) 560
BceAI ACGGC 2 cut(s) 5, 524
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 2 cut(s) 264, 325
BcoDI GTCTC 3 cut(s) 118, 493, 626
BcuI ACTAGT 1 cut(s) 314
BfaI CTAG 2 cut(s) 315, 417
BfmI CTRYAG 2 cut(s) 492, 725
BfuAI ACCTGC 1 cut(s) 669
BisI GCNGC 6 cut(s) 6, 35, 38, 87, 529, 594
BlsI GCNGC 6 cut(s) 7, 36, 39, 88, 530, 595
Bme1390I CCNGG 2 cut(s) 264, 325
Bme18I GGWCC 3 cut(s) 48, 236, 658
BmeT110I CYCGRG 1 cut(s) 629
BmgT120I GGNCC 4 cut(s) 48, 236, 569, 658
BmiI GGNNCC 3 cut(s) 238, 329, 627
BmrFI CCNGG 2 cut(s) 264, 325
BmsI GCATC 3 cut(s) 209, 337, 419
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BsaJI CCNNGG 2 cut(s) 240, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 518
Bse1I ACTGG 1 cut(s) 287
Bse21I CCTNAGG 1 cut(s) 654
Bse3DI GCAATG 1 cut(s) 698
BseBI CCWGG 2 cut(s) 264, 325
BseDI CCNNGG 2 cut(s) 240, 323
BseLI CCNNNNNNNGG 1 cut(s) 518
BseMI GCAATG 1 cut(s) 698
BseNI ACTGG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 2 cut(s) 212, 262
BseX3I CGGCCG 1 cut(s) 509
BseXI GCAGC 2 cut(s) 49, 580
Bsh1285I CGRYCG 1 cut(s) 512
BshFI GGCC 4 cut(s) 5, 135, 511, 570
BsiEI CGRYCG 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 262
BsiHKCI CYCGRG 1 cut(s) 629
BsiSI CCGG 1 cut(s) 512
BslI CCNNNNNNNGG 1 cut(s) 518
BsmAI GTCTC 3 cut(s) 118, 493, 626
BsmBI CGTCTC 2 cut(s) 118, 493
BsnI GGCC 4 cut(s) 5, 135, 511, 570
BsoBI CYCGRG 1 cut(s) 629
Bsp1286I GDGCHC 2 cut(s) 212, 262
Bsp143I GATC 3 cut(s) 327, 376, 625
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 7 cut(s) 6, 13, 35, 57, 87, 425, 529
BspANI GGCC 4 cut(s) 5, 135, 511, 570
BspLI GGNNCC 3 cut(s) 238, 329, 627
BspMI ACCTGC 1 cut(s) 669
BspPI GGATC 4 cut(s) 322, 335, 620, 633
BsrBI CCGCTC 2 cut(s) 59, 89
BsrDI GCAATG 1 cut(s) 698
BsrI ACTGG 1 cut(s) 287
BssECI CCNNGG 2 cut(s) 240, 323
BssMI GATC 3 cut(s) 327, 376, 625
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 2 cut(s) 264, 325
Bst6I CTCTTC 1 cut(s) 636
BstDEI CTNAG 2 cut(s) 204, 654
BstDSI CCRYGG 1 cut(s) 240
BstKTI GATC 3 cut(s) 330, 379, 628
BstMAI GTCTC 3 cut(s) 118, 493, 626
BstMBI GATC 3 cut(s) 327, 376, 625
BstMCI CGRYCG 1 cut(s) 512
BstMWI GCNNNNNNNGC 3 cut(s) 65, 197, 599
BstNI CCWGG 2 cut(s) 264, 325
BstNSI RCATGY 1 cut(s) 535
BstSCI CCNGG 2 cut(s) 262, 323
BstSFI CTRYAG 2 cut(s) 492, 725
BstSLI GKGCMC 2 cut(s) 212, 262
BstV1I GCAGC 2 cut(s) 49, 580
BstX2I RGATCY 2 cut(s) 327, 625
BstYI RGATCY 2 cut(s) 327, 625
BstZI CGGCCG 1 cut(s) 509
Bsu36I CCTNAGG 1 cut(s) 654
BsuRI GGCC 4 cut(s) 5, 135, 511, 570
BtgI CCRYGG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 276
BveI ACCTGC 1 cut(s) 669
Cfr13I GGNCC 4 cut(s) 48, 236, 569, 658
CsiI ACCWGGT 1 cut(s) 262
Csp6I GTAC 1 cut(s) 431
CviAII CATG 4 cut(s) 52, 241, 442, 532
CviQI GTAC 1 cut(s) 431
DdeI CTNAG 2 cut(s) 204, 654
DpnI GATC 3 cut(s) 329, 378, 627
DpnII GATC 3 cut(s) 327, 376, 625
EaeI YGGCCR 3 cut(s) 3, 133, 509
EagI CGGCCG 1 cut(s) 509
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
EclXI CGGCCG 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 240
Eco47I GGWCC 3 cut(s) 48, 236, 658
Eco52I CGGCCG 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 401
Eco81I CCTNAGG 1 cut(s) 654
Eco88I CYCGRG 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 658
EcoRII CCWGG 2 cut(s) 262, 323
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
Esp3I CGTCTC 2 cut(s) 118, 493
FaeI CATG 4 cut(s) 55, 244, 445, 535
FatI CATG 4 cut(s) 51, 240, 441, 531
FauNDI CATATG 1 cut(s) 187
Fnu4HI GCNGC 6 cut(s) 6, 35, 38, 87, 529, 594
Fsp4HI GCNGC 6 cut(s) 6, 35, 38, 87, 529, 594
FspBI CTAG 2 cut(s) 315, 417
GluI GCNGC 6 cut(s) 6, 35, 38, 87, 529, 594
HaeIII GGCC 4 cut(s) 5, 135, 511, 570
HapII CCGG 1 cut(s) 512
Hin1II CATG 4 cut(s) 55, 244, 445, 535
HinfI GANTC 1 cut(s) 72
HpaII CCGG 1 cut(s) 512
HphI GGTGA 2 cut(s) 85, 552
Hpy166II GTNNAC 1 cut(s) 260
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 2 cut(s) 62, 629
Hpy8I GTNNAC 1 cut(s) 260
HpyAV CCTTC 2 cut(s) 39, 376
HpyCH4IV ACGT 1 cut(s) 429
HpyCH4V TGCA 6 cut(s) 200, 260, 336, 410, 596, 602
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 197, 599
HpyF3I CTNAG 2 cut(s) 204, 654
HpySE526I ACGT 1 cut(s) 429
Hsp92II CATG 4 cut(s) 55, 244, 445, 535
Kzo9I GATC 3 cut(s) 327, 376, 625
Lsp1109I GCAGC 2 cut(s) 49, 580
LweI GCATC 3 cut(s) 209, 337, 419
MabI ACCWGGT 1 cut(s) 262
MaeI CTAG 2 cut(s) 315, 417
MaeII ACGT 1 cut(s) 429
MalI GATC 3 cut(s) 329, 378, 627
MbiI CCGCTC 2 cut(s) 59, 89
MboI GATC 3 cut(s) 327, 376, 625
MboII GAAGA 3 cut(s) 18, 54, 653
MfeI CAATTG 1 cut(s) 372
MflI RGATCY 2 cut(s) 327, 625
MhlI GDGCHC 2 cut(s) 212, 262
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 6 cut(s) 150, 252, 372, 402, 545, 585
MluNI TGGCCA 1 cut(s) 135
MnlI CCTC 5 cut(s) 89, 115, 118, 598, 660
Mox20I TGGCCA 1 cut(s) 135
MscI TGGCCA 1 cut(s) 135
MseI TTAA 4 cut(s) 308, 362, 536, 669
Msp20I TGGCCA 1 cut(s) 135
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 2 cut(s) 264, 325
MunI CAATTG 1 cut(s) 372
MvaI CCWGG 2 cut(s) 264, 325
MwoI GCNNNNNNNGC 3 cut(s) 65, 197, 599
NcoI CCATGG 1 cut(s) 240
NdeI CATATG 1 cut(s) 187
NdeII GATC 3 cut(s) 327, 376, 625
NlaIII CATG 4 cut(s) 55, 244, 445, 535
NlaIV GGNNCC 3 cut(s) 238, 329, 627
NspI RCATGY 1 cut(s) 535
PfeI GAWTC 1 cut(s) 72
PflFI GACNNNGTC 1 cut(s) 52
PkrI GCNGC 6 cut(s) 7, 36, 39, 88, 530, 595
PpuMI RGGWCCY 1 cut(s) 658
Psp5II RGGWCCY 1 cut(s) 658
Psp6I CCWGG 2 cut(s) 262, 323
PspGI CCWGG 2 cut(s) 262, 323
PspN4I GGNNCC 3 cut(s) 238, 329, 627
PspPI GGNCC 4 cut(s) 48, 236, 569, 658
PspPPI RGGWCCY 1 cut(s) 658
PsuI RGATCY 2 cut(s) 327, 625
PsyI GACNNNGTC 1 cut(s) 52
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
SaqAI TTAA 4 cut(s) 308, 362, 536, 669
SatI GCNGC 6 cut(s) 6, 35, 38, 87, 529, 594
Sau3AI GATC 3 cut(s) 327, 376, 625
Sau96I GGNCC 4 cut(s) 48, 236, 569, 658
ScrFI CCNGG 2 cut(s) 264, 325
SduI GDGCHC 2 cut(s) 212, 262
SetI ASST 7 cut(s) 268, 292, 432, 499, 565, 663, 684
SexAI ACCWGGT 1 cut(s) 262
SfaNI GCATC 3 cut(s) 209, 337, 419
SfcI CTRYAG 2 cut(s) 492, 725
SinI GGWCC 3 cut(s) 48, 236, 658
SpeI ACTAGT 1 cut(s) 314
Sse9I AATT 6 cut(s) 150, 252, 372, 402, 545, 585
SsiI CCGC 7 cut(s) 6, 13, 35, 57, 87, 425, 529
SspMI CTAG 2 cut(s) 315, 417
StyD4I CCNGG 2 cut(s) 262, 323
StyI CCWWGG 1 cut(s) 240
TaiI ACGT 1 cut(s) 432
TaqI TCGA 1 cut(s) 63
TasI AATT 6 cut(s) 150, 252, 372, 402, 545, 585
TauI GCSGC 4 cut(s) 8, 37, 89, 531
TfiI GAWTC 1 cut(s) 72
Tru1I TTAA 4 cut(s) 308, 362, 536, 669
Tru9I TTAA 4 cut(s) 308, 362, 536, 669
TscAI CASTG 1 cut(s) 283
TseI GCWGC 2 cut(s) 37, 593
TspDTI ATGAA 3 cut(s) 596, 679, 687
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 1 cut(s) 283
Tth111I GACNNNGTC 1 cut(s) 52
VneI GTGCAC 1 cut(s) 258
VpaK11BI GGWCC 3 cut(s) 48, 236, 658
XapI RAATTY 1 cut(s) 585
XceI RCATGY 1 cut(s) 535
XspI CTAG 2 cut(s) 315, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.